LOC111683565 cDNA ORF clone, Lucilia cuprina(Australian sheep blowfly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC111683565
Entrez Gene ID	111683565
Full Name	E3 ubiquitin-protein ligase RING1
Gene Type	protein-coding
Organism	Lucilia cuprina(Australian sheep blowfly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023445649.1	XP_023301417.1	E3 ubiquitin-protein ligase RING1

Pathways from BioSystems
Gene Ontology

The following LOC111683565 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC111683565 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLu55064	XM_023445649.1 Latest version!	Lucilia cuprina E3 ubiquitin-protein ligase RING1 (LOC111683565), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC111683565 ( XM_023445649.1 ) cDNA ORF clone, Lucilia cuprina(Australian sheep blowfly) -> XP_023301417.1 Lucilia cuprina E3 ubiquitin-protein ligase RING1 (LOC111683565), mRNA.

Price & Availability↑

CloneID	OLu55064
Clone ID Related Accession (Same CDS sequence)	XM_023445649.1
Accession Version	XM_023445649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-04
Organism	Lucilia cuprina(Australian sheep blowfly)
Product	E3 ubiquitin-protein ligase RING1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019410947.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Lucilia cuprina Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTT TGGAACCAGC ACAAAATAAG ACATGGGATT TGTCTTTGTA CGAACTACAG 
CGCAAACCGC AAGAAATTAT TACAGACAAT ACAGAAATAG CAGTTTCACC CAGAAGTTTA 
CACAGTGAAC TAATGTGTCC CATATGTTTG GATATGCTGA AAAAAACAAT GACTACTAAG 
GAGTGTCTGC ATCGATTTTG CCAAGATTGT ATTATAACTG CATTACGTTC GGGCAACAAA 
GAGTGCCCGA CTTGTCGTAA GAAATTGGTA TCGAAAAGAT CTTTGAGACC AGATCCAAAT 
TTCGATTTAT TAATCTCAAA AATTTATCCC AGCCGAGAAG AATATGAAGC CATTCAGGAA 
AAAGTAATGG CGAAATTCAA TCAAACACAG TCGCAGCAAG CTTTGGTAAA TTCTATAAAC 
GAAGGCATTA AATTGCAAAG CCAAAACAGG CCTCAGCGTT TTAGAACCAA GGGAAGTGGT 
GGCGGAGGTG GTGCTGCTGG TGGCGGTGGT CGTGCAGCAG GTGCTAATCA TAATTCTAAC 
AATGCACATA ATGTATCAAA TGAAAGTAAT ACCAATGGCA ATGAAAGTGA TACAAATACA 
AGAGAAAGTA CCACAACAGC TCCACTAGCG GCTACTGTAG CTATAAATGC TCCAACAACT 
ACTGCTACTA CACATTCCAA TACTACAATT GCTGCTGCTG TTTCGTCTGT AACCGGTAGC 
ATTGCAGCAA GTACTTCTTC CACCCGCATG GATGATGGCG GCTCTAATCC ACCCTCTGTA 
CGTTCCACTC CCTCCCCAGT ACCTTCCAAT TCAAGTAGCT CAAAACCTAA ACGAGCGATG 
TCCGTTTTAA CATCTGAACG GTCAGAAGAA TCAGAATCTG ATTCTCAGAT GGACTGTCGC 
ACTGAGGGCG ACTCAAATAT AGACACAGAA GGTGAAGGTA ACGGTGAATT GGGTATTAAT 
GATGAAATTG AATTGGTTTT CAAGCCTCAT CCCACAGAAA TGGCTGGTGA TAATCAGTTA 
ATGAAGGCTT TGAAAGAAAA TTGCATACGT TATATAAAGA CGACGGCAAA TGCCACAGTT 
GACCACTTGA GCAAGTATTT GGCTTTACGT TTAACCCTTG ATTTGGGTTC TGAGCTACCC 
GAAGCTTATC GTATGCTTAA CTTTTGCATC TATGTTGCCC CCCAACCCTC TCAATTGGTT 
ATTTTAAATG GCAATCAAAC ATTACATCAA GTTAATGAGA AATTCTGGAA AGTTAATAAA 
CCCATGGAAA TGTTTTATTC CTGGAAGAAA TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023301417.1
CDS	121..1416
Translation	MASLEPAQNKTWDLSLYELQRKPQEIITDNTEIAVSPRSLHSELMCPICLDMLKKTMTTKECLHRFCQDCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDLLISKIYPSREEYEAIQEKVMAKFNQTQSQQALVNSINEGIKLQSQNRPQRFRTKGSGGGGGAAGGGGRAAGANHNSNNAHNVSNESNTNGNESDTNTRESTTTAPLAATVAINAPTTTATTHSNTTIAAAVSSVTGSIAASTSSTRMDDGGSNPPSVRSTPSPVPSNSSSSKPKRAMSVLTSERSEESESDSQMDCRTEGDSNIDTEGEGNGELGINDEIELVFKPHPTEMAGDNQLMKALKENCIRYIKTTANATVDHLSKYLALRLTLDLGSELPEAYRMLNFCIYVAPQPSQLVILNGNQTLHQVNEKFWKVNKPMEMFYSWKKS

Target ORF information:

RefSeq Version	XM_023445649.1
Organism	Lucilia cuprina(Australian sheep blowfly)
Definition	Lucilia cuprina E3 ubiquitin-protein ligase RING1 (LOC111683565), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023445649.1

ORF Insert Sequence:

ATGGCTTCTT TGGAACCAGC ACAAAATAAG ACATGGGATT TGTCTTTGTA CGAACTACAG 
CGCAAACCGC AAGAAATTAT TACAGACAAT ACAGAAATAG CAGTTTCACC CAGAAGTTTA 
CACAGTGAAC TAATGTGTCC CATATGTTTG GATATGCTGA AAAAAACAAT GACTACTAAG 
GAGTGTCTGC ATCGATTTTG CCAAGATTGT ATTATAACTG CATTACGTTC GGGCAACAAA 
GAGTGCCCGA CTTGTCGTAA GAAATTGGTA TCGAAAAGAT CTTTGAGACC AGATCCAAAT 
TTCGATTTAT TAATCTCAAA AATTTATCCC AGCCGAGAAG AATATGAAGC CATTCAGGAA 
AAAGTAATGG CGAAATTCAA TCAAACACAG TCGCAGCAAG CTTTGGTAAA TTCTATAAAC 
GAAGGCATTA AATTGCAAAG CCAAAACAGG CCTCAGCGTT TTAGAACCAA GGGAAGTGGT 
GGCGGAGGTG GTGCTGCTGG TGGCGGTGGT CGTGCAGCAG GTGCTAATCA TAATTCTAAC 
AATGCACATA ATGTATCAAA TGAAAGTAAT ACCAATGGCA ATGAAAGTGA TACAAATACA 
AGAGAAAGTA CCACAACAGC TCCACTAGCG GCTACTGTAG CTATAAATGC TCCAACAACT 
ACTGCTACTA CACATTCCAA TACTACAATT GCTGCTGCTG TTTCGTCTGT AACCGGTAGC 
ATTGCAGCAA GTACTTCTTC CACCCGCATG GATGATGGCG GCTCTAATCC ACCCTCTGTA 
CGTTCCACTC CCTCCCCAGT ACCTTCCAAT TCAAGTAGCT CAAAACCTAA ACGAGCGATG 
TCCGTTTTAA CATCTGAACG GTCAGAAGAA TCAGAATCTG ATTCTCAGAT GGACTGTCGC 
ACTGAGGGCG ACTCAAATAT AGACACAGAA GGTGAAGGTA ACGGTGAATT GGGTATTAAT 
GATGAAATTG AATTGGTTTT CAAGCCTCAT CCCACAGAAA TGGCTGGTGA TAATCAGTTA 
ATGAAGGCTT TGAAAGAAAA TTGCATACGT TATATAAAGA CGACGGCAAA TGCCACAGTT 
GACCACTTGA GCAAGTATTT GGCTTTACGT TTAACCCTTG ATTTGGGTTC TGAGCTACCC 
GAAGCTTATC GTATGCTTAA CTTTTGCATC TATGTTGCCC CCCAACCCTC TCAATTGGTT 
ATTTTAAATG GCAATCAAAC ATTACATCAA GTTAATGAGA AATTCTGGAA AGTTAATAAA 
CCCATGGAAA TGTTTTATTC CTGGAAGAAA TCATAA