RFWD2 cDNA ORF clone, Latimeria chalumnae(coelacanth)

Gene
Clones
gRNAs

Summary

Gene Symbol	RFWD2
Entrez Gene ID	102363850
Full Name	COP1, E3 ubiquitin ligase
Synonyms	RFWD2
Gene Type	protein-coding
Organism	Latimeria chalumnae(coelacanth)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005996835.2	XP_005996897.1	E3 ubiquitin-protein ligase RFWD2

Pathways from BioSystems
Gene Ontology

The following RFWD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFWD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa27715	XM_005996835.2 Latest version!	Latimeria chalumnae ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RFWD2 ( XM_005996835.2 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_005996897.1 Latimeria chalumnae ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), mRNA.

Price & Availability↑

CloneID	OLa27715
Clone ID Related Accession (Same CDS sequence)	XM_005996835.2
Accession Version	XM_005996835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	E3 ubiquitin-protein ligase RFWD2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005819347.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_005996835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCGA GCCGCCAGCA GCAGCAGTGT CCCGGGCAGG GTAGCAGCGG GACAGCGGTC 
CCCGCGGCCG CGCCATCCTC CAGCTCGGGG GCCGGCCGGC CGGGGACCTC AGCTGGACAG 
GCAGCAGTAA CCGCGGCCGC CGCAGCCTCA GGTTCGGGTG TGGGGCTGGC CCGGGTGGGA 
GCGGGCCGGG CCGGTAGCAG TAGCAGTAGT AGCTCCTCAT CCTCGCAGTC GGGTAGCAGG 
AAGCGGCCGC TGCTGACTCC GCTCTGTAAC GGGCTGCTGA ACTCGTATGA GGACAAGAGC 
AACGATTTCG TCTGCCCAAT CTGTTTTGAG ATGATCGAGG AAGCGTACAT GACAAAATGT 
GGGCACAGCT TCTGTTCAAA GTGTATTCGA CAGAGCCTGG AAGACAGTAA CAGATGTCCC 
AAATGTAATT ATGTGGTAGA CAATATAGAT CAGCTCTTCC CCAACTTCTT AGTGAATGAG 
CTAATCTTGA AACAGAAGCA AAGGTTTGAA GACAAACGAC TCAAACTGGA TCACTCTAAT 
GGGCACAAGT GGCAGATCTT CCAGGATGTC CTGGGCACTG ACCAGGAAAG TTTGGACCTG 
GCCAAGATAA ACCAGATACT GGAACTGCTA GTGCAGAAAA AGAAGCAGCT GGAGGCGGAA 
TCCCAAGCTG CCCAACTTCA GATCTTGATG GAGTTCCTCA AAGAGGCTAG AAGAAACAAG 
AGAGAGCAAC TAGAGCAGAT CCAGAAGGAG CTGAGTGTTT TGGAAGAGGA TATCAAGCGG 
GTGGAGGAAA TGAGTGGTTT GTATTCCCCT GTGAGTGAGA TAGATCAGTC CCAGAACTTG 
GACAGCACTG TGCCTCAGTT TGAAGCTCCA TCTCCCTCAC ACAGTATTAT TGACTCTACA 
GAATACACAC AGCATCCTGG ATTCAGTGGC ACATCACAGG GTAAGAGGCA GCCGTGGTAC 
AACAGCACAT TGGCATCTAG ACGGAAACGG CTAACAGCAC ACTTTGAAGA CCTGGAGCAG 
TGCTATTTCT CCACCCGGAT GTCTCGTATA TCAGATGACA GCAGGATAGT GAACCAGCTG 
GATGAGTTTC AGGAGTGCTT GGCAAAATTC ACGCGTTACA ATTCCGTTCG ACCTCTCGCC 
ACGCTGTCCT ACGCCAGTGA CCTATACAAT GGCTCCAGCA TAGTCTCTAG TATTGAGTTT 
GACCGGGATT GCGATTACTT TGCTATTGCT GGAGTGACCA AGAAGATCAA AGTGTACGAA 
TACGGTACAG TGATCCAGGA TGCTGTGGAC ATTCACTACC CTGTCATTGA GATGACCTGC 
AACTCCAAAA TCAGCTGTAT CAGCTGGAGT AGCTACCACA AGAACTTGCT GGCCAGCAGT 
GACTATGAGG GCACAGTCAT CCTATGGGAT GGTTTCACGG GTCAGAGGTC AAAGGTCTAT 
CAGGAGCATG AGAAAAGGTG CTGGAGTGTC GATTTTAACC TGATGGACCC CAAACTTCTA 
GCTTCAGGCT CTGATGATGC TAAAGTGAAG TTATGGTCAA CCAACCTGGA CAACTCTGTA 
GCCAGTATTG AAGCCAAGGC AAATGTTTGC TGTGTCAAGT TCAGCCCTAC GTCCAGGTAC 
CATCTTGCAT TCGGCTGTGC AGATCACTGC GTTCACTACT ATGACCTTCG CAACACTAAA 
CAGCCTATCA TGGTTTTCAA AGGCCATCGG AAGGCCGTCT CCTATGCAAA GTTTGTCAGT 
GGAGAAGAAA TCGTTTCTGC CTCCACAGAC AGCCAACTAA AGCTGTGGAA CGTGAACAAA 
CCTCACTGCC TACGGTCTTT CAAGGGTCAC ATCAACGAGA AGAATTTTGT GGGGCTGGCA 
TCCAACGGAG ATTACATTGC CTGTGGCTCT GAAAACAACT CCCTTTACTT GTATTACAAG 
GGTCTTTCCA AGACGCTGCT GACATTTAAA TTTGACACAG TGAAGAGTGT CCTGGACAAA 
GACAAGAAAG AAGATGACAC AAATGAGTTT GTGAGCGCTG TGTGCTGGAG GGCCCGGCCG 
GATGGGGAGT CCAATGTGCT AATAGCTGCC AACAGTCAGG GTACAATCAA GGTACTGGAG 
CTGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005996897.1
CDS	236..2344
Translation	MSASRQQQQCPGQGSSGTAVPAAAPSSSSGAGRPGTSAGQAAVTAAAAASGSGVGLARVGAGRAGSSSSSSSSSSQSGSRKRPLLTPLCNGLLNSYEDKSNDFVCPICFEMIEEAYMTKCGHSFCSKCIRQSLEDSNRCPKCNYVVDNIDQLFPNFLVNELILKQKQRFEDKRLKLDHSNGHKWQIFQDVLGTDQESLDLAKINQILELLVQKKKQLEAESQAAQLQILMEFLKEARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEIDQSQNLDSTVPQFEAPSPSHSIIDSTEYTQHPGFSGTSQGKRQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRIVNQLDEFQECLAKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPVIEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPTSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVNKPHCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDKKEDDTNEFVSAVCWRARPDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_005996835.2
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005996835.2

ORF Insert Sequence:

ATGTCAGCGA GCCGCCAGCA GCAGCAGTGT CCCGGGCAGG GTAGCAGCGG GACAGCGGTC 
CCCGCGGCCG CGCCATCCTC CAGCTCGGGG GCCGGCCGGC CGGGGACCTC AGCTGGACAG 
GCAGCAGTAA CCGCGGCCGC CGCAGCCTCA GGTTCGGGTG TGGGGCTGGC CCGGGTGGGA 
GCGGGCCGGG CCGGTAGCAG TAGCAGTAGT AGCTCCTCAT CCTCGCAGTC GGGTAGCAGG 
AAGCGGCCGC TGCTGACTCC GCTCTGTAAC GGGCTGCTGA ACTCGTATGA GGACAAGAGC 
AACGATTTCG TCTGCCCAAT CTGTTTTGAG ATGATCGAGG AAGCGTACAT GACAAAATGT 
GGGCACAGCT TCTGTTCAAA GTGTATTCGA CAGAGCCTGG AAGACAGTAA CAGATGTCCC 
AAATGTAATT ATGTGGTAGA CAATATAGAT CAGCTCTTCC CCAACTTCTT AGTGAATGAG 
CTAATCTTGA AACAGAAGCA AAGGTTTGAA GACAAACGAC TCAAACTGGA TCACTCTAAT 
GGGCACAAGT GGCAGATCTT CCAGGATGTC CTGGGCACTG ACCAGGAAAG TTTGGACCTG 
GCCAAGATAA ACCAGATACT GGAACTGCTA GTGCAGAAAA AGAAGCAGCT GGAGGCGGAA 
TCCCAAGCTG CCCAACTTCA GATCTTGATG GAGTTCCTCA AAGAGGCTAG AAGAAACAAG 
AGAGAGCAAC TAGAGCAGAT CCAGAAGGAG CTGAGTGTTT TGGAAGAGGA TATCAAGCGG 
GTGGAGGAAA TGAGTGGTTT GTATTCCCCT GTGAGTGAGA TAGATCAGTC CCAGAACTTG 
GACAGCACTG TGCCTCAGTT TGAAGCTCCA TCTCCCTCAC ACAGTATTAT TGACTCTACA 
GAATACACAC AGCATCCTGG ATTCAGTGGC ACATCACAGG GTAAGAGGCA GCCGTGGTAC 
AACAGCACAT TGGCATCTAG ACGGAAACGG CTAACAGCAC ACTTTGAAGA CCTGGAGCAG 
TGCTATTTCT CCACCCGGAT GTCTCGTATA TCAGATGACA GCAGGATAGT GAACCAGCTG 
GATGAGTTTC AGGAGTGCTT GGCAAAATTC ACGCGTTACA ATTCCGTTCG ACCTCTCGCC 
ACGCTGTCCT ACGCCAGTGA CCTATACAAT GGCTCCAGCA TAGTCTCTAG TATTGAGTTT 
GACCGGGATT GCGATTACTT TGCTATTGCT GGAGTGACCA AGAAGATCAA AGTGTACGAA 
TACGGTACAG TGATCCAGGA TGCTGTGGAC ATTCACTACC CTGTCATTGA GATGACCTGC 
AACTCCAAAA TCAGCTGTAT CAGCTGGAGT AGCTACCACA AGAACTTGCT GGCCAGCAGT 
GACTATGAGG GCACAGTCAT CCTATGGGAT GGTTTCACGG GTCAGAGGTC AAAGGTCTAT 
CAGGAGCATG AGAAAAGGTG CTGGAGTGTC GATTTTAACC TGATGGACCC CAAACTTCTA 
GCTTCAGGCT CTGATGATGC TAAAGTGAAG TTATGGTCAA CCAACCTGGA CAACTCTGTA 
GCCAGTATTG AAGCCAAGGC AAATGTTTGC TGTGTCAAGT TCAGCCCTAC GTCCAGGTAC 
CATCTTGCAT TCGGCTGTGC AGATCACTGC GTTCACTACT ATGACCTTCG CAACACTAAA 
CAGCCTATCA TGGTTTTCAA AGGCCATCGG AAGGCCGTCT CCTATGCAAA GTTTGTCAGT 
GGAGAAGAAA TCGTTTCTGC CTCCACAGAC AGCCAACTAA AGCTGTGGAA CGTGAACAAA 
CCTCACTGCC TACGGTCTTT CAAGGGTCAC ATCAACGAGA AGAATTTTGT GGGGCTGGCA 
TCCAACGGAG ATTACATTGC CTGTGGCTCT GAAAACAACT CCCTTTACTT GTATTACAAG 
GGTCTTTCCA AGACGCTGCT GACATTTAAA TTTGACACAG TGAAGAGTGT CCTGGACAAA 
GACAAGAAAG AAGATGACAC AAATGAGTTT GTGAGCGCTG TGTGCTGGAG GGCCCGGCCG 
GATGGGGAGT CCAATGTGCT AATAGCTGCC AACAGTCAGG GTACAATCAA GGTACTGGAG 
CTGGTATGA