IL12B cDNA ORF clone, Latimeria chalumnae(coelacanth)

Gene
Clones
gRNAs

Summary

Gene Symbol	IL12B
Entrez Gene ID	102348110
Full Name	interleukin 12B
Gene Type	protein-coding
Organism	Latimeria chalumnae(coelacanth)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006011399.2	XP_006011461.2	interleukin-12 subunit beta

Pathways from BioSystems
Gene Ontology

The following IL12B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL12B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa42496	XM_006011399.2 Latest version!	Latimeria chalumnae interleukin 12B (IL12B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL12B ( XM_006011399.2 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_006011461.2 Latimeria chalumnae interleukin 12B (IL12B), mRNA.

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CloneID	OLa42496
Clone ID Related Accession (Same CDS sequence)	XM_006011399.2
Accession Version	XM_006011399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	interleukin-12 subunit beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005821736.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_006011399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTCC TATGGATTAT ATTTTCCTTG TTATGCTGGT CTGTCAACCC ACTGGAAGGA 
AAATGGCTGT TCAAACCAAA CATTTATATT GTAGATTCAG AGATGACGCC TGATACACCA 
GCTGAACCTA TTACTTTGCA CTGTGATGCA CCCGAGGACC AAAATCCATC TGGAATTGTC 
TGGAAGAGAA ATTTTGGAGA AGGGCCATAC AAAATACCGG AGAAAGGCAA CACAATTACA 
ATCAATGTCA AGGACTATCC AGACGCAGGC AACTACACCT GCCATCTTGC GTCCAAAGAT 
GAAATAATAA ACTACACCGT CATTGCAATT CATGTGGTAA ACAAAGGCAA AAAAGAAGAG 
AATTCCAAGA AAATCCTCAA AAAAATTGAC GGCCCAAAGC AGACACGCTT TGTTGACTGT 
GAAGCACGAT CATACTGCGG GGCCTTCAAT TGCTCCTGGA CACTGGACAA GAAAACAGGA 
AAGGAGAATG TAATGTTTTC CATCAAATTT CTGGAAGGAT TCAAGTCAAA CAGAAGTGTA 
ATCTGTGATG AGCCCACAAC AACTGATGAC CTAACTTACA CAGCAGAGTG CCGAGATCAA 
AACTTCTGTC CATATGCTGA AGAGGATACG CCCATCGATA TTTCCCTCCA GGTTATACAA 
GGGATTCAGT ATGAAAATTA CACTGAAACG TTCTTCATCC GAGATATTGT AAAACCTGAT 
CCACCACAGC ATCTCACCAT GAAGAAGGAA AATAATAATT TAACCATTTC CTGGCAGTAC 
CCAGAATTCT GGTCAAAACC ACATTCTTAC TTCCCACTGA CCTTCGAAGT CAAAGTCACA 
GAAGAACAGA AAGGCAGAAG GAACAGAAAA AACAATGAGA AAAGGGACTG TAGTCACCTT 
GCAAGAGCCA AACGGTTCAC GGAGCACAAT CATGCTGAGA AAGTAAACAG CTATTTTACT 
GATGAAGAGG AAAAGGTCAT CACAGTGAAT CCTGGAAAGA ACTATTGTGT ATGTGTTCGA 
GCAAAGGACA GATACTTCAA TTCAAGATGG AGTTCTTGGA ACTGTGAAAA CCTGGTGAAT 
AAAAGGAAGA AAAGAAGCGG AAACAAGAGA AAGAACAGAG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006011461.2
CDS	23..1147
Translation	MHFLWIIFSLLCWSVNPLEGKWLFKPNIYIVDSEMTPDTPAEPITLHCDAPEDQNPSGIVWKRNFGEGPYKIPEKGNTITINVKDYPDAGNYTCHLASKDEIINYTVIAIHVVNKGKKEENSKKILKKIDGPKQTRFVDCEARSYCGAFNCSWTLDKKTGKENVMFSIKFLEGFKSNRSVICDEPTTTDDLTYTAECRDQNFCPYAEEDTPIDISLQVIQGIQYENYTETFFIRDIVKPDPPQHLTMKKENNNLTISWQYPEFWSKPHSYFPLTFEVKVTEEQKGRRNRKNNEKRDCSHLARAKRFTEHNHAEKVNSYFTDEEEKVITVNPGKNYCVCVRAKDRYFNSRWSSWNCENLVNKRKKRSGNKRKNRV

Target ORF information:

RefSeq Version	XM_006011399.2
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae interleukin 12B (IL12B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006011399.2

ORF Insert Sequence:

ATGCATTTCC TATGGATTAT ATTTTCCTTG TTATGCTGGT CTGTCAACCC ACTGGAAGGA 
AAATGGCTGT TCAAACCAAA CATTTATATT GTAGATTCAG AGATGACGCC TGATACACCA 
GCTGAACCTA TTACTTTGCA CTGTGATGCA CCCGAGGACC AAAATCCATC TGGAATTGTC 
TGGAAGAGAA ATTTTGGAGA AGGGCCATAC AAAATACCGG AGAAAGGCAA CACAATTACA 
ATCAATGTCA AGGACTATCC AGACGCAGGC AACTACACCT GCCATCTTGC GTCCAAAGAT 
GAAATAATAA ACTACACCGT CATTGCAATT CATGTGGTAA ACAAAGGCAA AAAAGAAGAG 
AATTCCAAGA AAATCCTCAA AAAAATTGAC GGCCCAAAGC AGACACGCTT TGTTGACTGT 
GAAGCACGAT CATACTGCGG GGCCTTCAAT TGCTCCTGGA CACTGGACAA GAAAACAGGA 
AAGGAGAATG TAATGTTTTC CATCAAATTT CTGGAAGGAT TCAAGTCAAA CAGAAGTGTA 
ATCTGTGATG AGCCCACAAC AACTGATGAC CTAACTTACA CAGCAGAGTG CCGAGATCAA 
AACTTCTGTC CATATGCTGA AGAGGATACG CCCATCGATA TTTCCCTCCA GGTTATACAA 
GGGATTCAGT ATGAAAATTA CACTGAAACG TTCTTCATCC GAGATATTGT AAAACCTGAT 
CCACCACAGC ATCTCACCAT GAAGAAGGAA AATAATAATT TAACCATTTC CTGGCAGTAC 
CCAGAATTCT GGTCAAAACC ACATTCTTAC TTCCCACTGA CCTTCGAAGT CAAAGTCACA 
GAAGAACAGA AAGGCAGAAG GAACAGAAAA AACAATGAGA AAAGGGACTG TAGTCACCTT 
GCAAGAGCCA AACGGTTCAC GGAGCACAAT CATGCTGAGA AAGTAAACAG CTATTTTACT 
GATGAAGAGG AAAAGGTCAT CACAGTGAAT CCTGGAAAGA ACTATTGTGT ATGTGTTCGA 
GCAAAGGACA GATACTTCAA TTCAAGATGG AGTTCTTGGA ACTGTGAAAA CCTGGTGAAT 
AAAAGGAAGA AAAGAAGCGG AAACAAGAGA AAGAACAGAG TTTAG