cep295 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	cep295
Entrez Gene ID	104938720
Full Name	centrosomal protein 295
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010755163.2	XP_010753465.2	centrosomal protein of 295 kDa isoform X1
XM_027279900.1	XP_027135701.1	centrosomal protein of 295 kDa isoform X1
XM_027279902.1	XP_027135703.1	centrosomal protein of 295 kDa isoform X3
XM_027279901.1	XP_027135702.1	centrosomal protein of 295 kDa isoform X2
XM_019255443.1	XP_019110988.1	centrosomal protein of 295 kDa isoform X2
XM_019255444.1	XP_019110989.1	centrosomal protein of 295 kDa isoform X3

Pathways from BioSystems
Gene Ontology

The following cep295 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cep295 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa221268	XM_027279900.1 Latest version!	Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa221269	XM_027279901.1 Latest version!	Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa221270	XM_027279902.1 Latest version!	Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OLa117478	XM_010755163.2 Latest version!	Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OLa117479	XM_019255443.1 Latest version!	Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OLa117480	XM_019255444.1 Latest version!	Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cep295 ( XM_027279900.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027135701.1 Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X1, mRNA.

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CloneID	OLa221268
Clone ID Related Accession (Same CDS sequence)	XM_027279900.1
Accession Version	XM_027279900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	centrosomal protein of 295 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040017.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA AAGTTTCCCA GCTCAGACTG AGCCCCAACG AGGAGGCCAG GCTGATCCGA 
GAGGAGCATG AGAGGAGGAG GAAGCTGCGA ATACAGCAGG TGCGGGAGCA GCAGCGGTAC 
ATCGCGCTGC AGATCCGCCG AGAGGTCGAG TTGAGGCGGC GGCGTGAGCT CGAGCAGCTG 
AAGGAGGAGC TGAGGGACGA CTGGGAGCGA CAGCAGAGGG AGAAACTCAA CACACTGCAG 
AAACTTTACC AGGAGAGCCT CCAGCTTCTC GGCCAAGGAC ACAGGAGCGC AAAAGAAAAC 
GAACCCGACC TGGCAGCCAT AGCTCAGAGG GAGGAGGAAA ATCACAGCAA AGCAGAGGAG 
CGTTATCGAG AAGCTCTGAA TGAGCTCAAA TCACAGAGGC TTAAAGACCA AGAGAGGCAG 
AGCCGTTCTA TCAATGCCAG GAAGAAGGCG CTACAGGCAG AAAAGGAGAG AGCGGCAAAA 
GTGGCCAGCC TCCCGCCGCC GCCGCCAAAC CCCATTCAGA ACATCGATTC AAAGAAGCCT 
CATGTGGTAA AGAAGTCTGA TGTGAGCGCC TTTGCTGCTA CACATTACCA CATGCCTGAG 
AGCACAGTGG ACAGAGAGGC AGACCCAACA CAGCCTAACG CTCACGAGGA AGCTGAGCTG 
GAGGCGAGGA GAATGCAGGA CCTCCAGAGG GAGGAGAAGC AGAGGAGAGA GGAGCAGCTG 
GAGAAGGCTC GTCTCAGAGG GAAGCAGGCT CTGAGGAGGG AACACCTCGT GCAGGACCGT 
GAGCGCTTGC TCTTGGAGCT GGAACACATG CAGCAGACAG ACCTGCTGAG GAGGAGACAG 
CAGGTGTCAC ACATGCCTGC TCAGATCTTC CAGCCTCTCT ACAAGAGACA GGAGATGAGG 
GAGGACTTCC AGAGGGAGAT GGAGTTCGCC TTTGAGGACA TGTACACTGG AGAGAGGAGG 
GTTAAAGGTG ATCTGGTGGT CCAGCTGGTA CCGGAGCCTC TGCCAGCTCT GTCCACAGGC 
AGCCAAGACC AGGACCAGGA GCTGGACATC ACGCTGGACG AAATCCCCAC ACCAGCAGCA 
GAAAACACAC ACCATGACAC TGAGCAAGAA GCTGGAAGCC CTGAGCAGGA AGCATCTGCA 
CGAGCAGAGA CCTCCAGACC TGCTCCCAGA CGGGCGTTGA AGAAACTCCT GGATCGTATC 
AGGAGCCAGC GGACTCACTG GAGCGAACAC AACGGTCACG TCCCCGCAGC CGACTCGCCG 
ACCGCCTTCA CCAATCAGAT ACCAGAGCAC GACACAACCA TCGACACGGG ATCTCTGACC 
AGTGAGGAGA AGGACAAACT CCCTGAACCC GCCCGCTCTC CACCCGCAGT TTCAGAGACA 
ACAGAGCCGT CAGCTGAAGC AGAAACTTCG GCTCCTGTCC GACTCCAAGA ATTTACAGAC 
GAACGAAGGA AAAGGGAGTT GGAGCTCGAG CAGGAGAAGC AGCAGCAGGT GGTGTTGATC 
CAGGAGTTAG AGGAGCAGAA GGCCAAACTG GAGCAGATGC TGTTTGAAGC ACAGCAGGAG 
AGAGAGCACC TAAAGGCTGC TGTGACCCAG GAAGTGCCTG TCCTCGACCA GGAAGTCACC 
TCCGTCTCCT CCGGGCTGTC CAGTGAGCTG GTGCCTCCTC CGGGTGAAGA TGAACACAGC 
AGAAGGATTC GAGAATACCA ACAGAGGCTG CTGGAACAGA ACAGAATTCA TCAGAGGTCG 
GTGGAGGTGG CTCGTCAGCG GCTGGAGGAA TACCAGCGAG CTCTACAGAT CCGTTACAGC 
ATGACCACCA AACAGTCGCT GCCCTCCGTC GTGCCTCCAG GACTCGTTCA CCCGCCGCTA 
CACAGTACTG GGCCTGTTCA TCTTCCTGCT CCTCCGTCAC TCCCAACAAC TCCTGCAGTG 
CCTGCATACG TCCCTGCCAG ACCACACCCC TCTGTGGAAG TCACTACAAG AGAGGCTGAC 
ATGGTGGCTT CACCTCCCCA CCTTCCTGGC TCCAGTTTGA GGGTTTGCTC CAGGTTGCTG 
CCAGATGAAG TGGAATCTGT TTGGAACAGA GCGAAGAACC AGAAACCCGA TGTTTCTACC 
TGGCTGACTG ACAACATAAT GGAGAGAGTG ACTGAGCACC TTCCAGACAG CGTGAAACCC 
GTCTCAGTCA CCATGAAGCC ACCGCCCCAC AAACTGTCCA CAACACGTGA GTCTACCAGC 
ATCCTGCTTC AGCCGACCTC TCATCCCATC CAAGTCATCG GCCCCAGCAT TGCAGATAGA 
ACACCTCTGT TTCCAGGCCA TGTAGCGGTT AAGCCGCATG TCTCCCTGCT GACTGGGACC 
CTGAGCTCCA GAGAGGACGA CATGGAGAGG CAGAGACGGG AGCTGCAGAG GCAACAAGAG 
GAGGAGGAGG AGGAGGAGAG GCAGAGATGG GAGCTGCAGG AGGTTCAGAG ACGATTGCTG 
GAGCAGAGGG AGGCAGTGGA AGTGCAACAG AGGCAGAAAG AAGAGGAGAG GCAGAGACAG 
GAGCTGCAGA GACGAATGCA GGAGCAAAGA GCGGCAGTAG AGCTCCAGCA GAAGCAGCAA 
GAAGAGGAGA GGCAGAGACG GTTGCTGGAG CAGAGGGAGG CGATGGAGGT GCAACAAAGG 
CAGACAGAGG AGGAGAAGCA GAGGCGGGAG CTGCAGGAGG TTCAGAGACG GTTGTTGGAG 
CAGAGGGAGG CAGTGGAGCT GCAGAAGAAG CAACAAGAAG AGGAGAGGCA GAGGCGGGAA 
GTGGAGATGG AGGAGATGAG GCGACAGAAG GAGACGTTAC AGGCTCTGAT TCATACTGAC 
GCACAGCCAG GTGCAGAGGT CTCCAGTGAA GTGCCGCTTT CAGAGAGCAT CGGTCCAACT 
CGACGTCAGT TACTTGCATC ACTGCTGCGA GCCATACAGG AAACTAACGG AGGAACTCTA 
TCACATCTAG AAGAGCCGCA GGAGGAAGAT GACTCTCCTC AACAACCATC ATCATCTTAC 
AGCGTCGAGA CTGACCCCGT CTTTCAGAGC AGTGTTCCTG CTGGACCTCT CCCAGCATCA 
GGCCTCCTGC CTCCTCCTCG AGCAGCGAAG CCTCCAGTGA CTCGCGTCAG GCTGGGCGTC 
ATGAAGATGA TGACTGAACA ACATGAGCTC AGTGCTATTC AAGAGGTGGA GACGCCAGTC 
AACAGCAGCC TGGTCACAGG TCTGGAGGAC AGTGTGAAGC TTCCTTCACA GACTGTGGGC 
TGGGATCCAC AGGAGGAGTC CGACTCGTCG GTGTCCTCTG ACAGGACTCT GCAGACGCCC 
TCTTGGTCCA GCAGCGAGCA GCGGCCTGCT GAAAGACGAG AGGCGAGCTC GAGGAGGTAC 
AGCCATCTGG ATCTGCTGAT GGAGGCTGGA ACATCTCCAG AATCCTCAGA GTCATCTGAT 
TCAGTCGTGA GGAAAACGTC ACGGCTTTCC TCTGACTCTG GCAGAGGAGC TGACTACTCC 
GGACCTGCGA TCACGAGCTA CAGATCCTTC ACAGAGTCTG CACAGAGGCC TCCTGACTCT 
GACTGCCTCT CCTCCACCAC CATCTCCAGC GGCAGCTACA TCACCACAGA CCCTGAACAA 
AACGAGCCAA ACGTAAACAC TGATAAATCT CTGCCCATCA GACGTCATGT GGAACCAGAT 
GGAGGAGCAA ATTCGCTGAA CGTCTCCTCT CCGTCGGGTC AAAACTTCTC CATGAACAGC 
TCGACTGCAG CCCGTCCTGC TCTGGATGTC GACTCTCTGT TTGGTGACAG CAGCATCCAG 
CGAATCATCG ACCGATACAC GAGAGAGCTC AACTTCTCCC TCAGCACCAC CGGGAGAGCC 
GCAGACAGTG AAGGCTCTCA TGTGGACGAT CCCGGCTCAG TCTCTCAGCA GTCTTTGGTT 
CGAGTCTCAG AGAGGAGTCG GTGTCTTCCC TCAGACGCAG CAGCAGCACA GAGGAGTGTC 
CAGGAATGGG ACAACACAGT CGATCCAATC CTGGAGCACT TCTCAGTTGA GGGCACAGAG 
CAGGACTCTT TCAGACCTCT GATTGGCCGG CTGGCAGACC AATCATCCTG CTTGGCTGTC 
GACCACAGGG ACTCGACCAT GGAGCAGCTG GTCGGTCAGC CATCGGCTCA CTCGTCCATG 
ATCGGTCAGC TGCCCGGGCT GCCGGTGGGC CTCGATCAAG GCGGATGGGA TTCCACTCTG 
AGCCGGATGA TTGGTCGACT CTCCCATCAG TCCAGCCCTC ATTGGCTGAG TGGAGGCCAC 
GACTTCTATG CGGGTCAGAT GGTGTCAGAG CGATCAGCCA CGTGGTTGGA TGAAGAGAGC 
CGGATGAGAC CGCTGGTCGG AGAGCTGGAT GAGTCCACTG GACACCACAG TGGGAGCTCA 
GGTGAAAGAA CTCGCGTGGA TCCCGATGTT TCCACTGAGG CCAGTGTGCC GTCATTCCCA 
GCGTTGCCTC CTGAAGCGTC GTCACACAGC GCCTCTGACC CGGCTGTGAA TCCACACCCC 
CAGGACCAGA ACCAAACCAG TCCAATGGAC CTGGGCCCAG AGCGGACTGA AGACTCGTTC 
CACCTGCTCG TGGCTGAAGT CACCCACAAC GAAACAGCCG AGCCCTCCGT CAACTTCCAC 
GCTGTGCCTG ACTCTCCTGA AGGTCGGCCA GCCAGCTGTG AACAGAGCGC CTCTGCACCG 
CCTGAAGAGT TTGAAACCCA CAACGACCCG TCCATCGAGT CTGAACTGTC TCCTGAGCGC 
CTCAGGACAG ACAAGCCGCC CAGTTGCCTG ACGCCGCCCC ACGCCTTACA TGAGTCTTTC 
TCCCAGCTCA TCACCTCCCA GCACCACCCC CATGAGGTGG AGCTAACCGC TCTGAGTCTG 
TCAAATCTCA CCATGTGTGA TGATGCGCTG ACTCTGAAGC TGCAGCCTGA GGAACCGTTC 
AGAGAGCTGG AGTCTGAGCA GACTGTTCGT CCTGCCTCAG AGAAGCTGTC GGAGGCTGGC 
GGTGAGAAAG GCATCCTGGA GCAGTCGGAG ATAACACTCG TGAGCCTGAC AGACACAACT 
CTGCAGGACC AAGAGACGAC CATCACCGAC GAAGACGGAG TCTGGGACGG CAAACATCCA 
GACAGGGTGA TGGAGGAAGG ACAGAAAGAG ACAGAGGAGT CGCACTCAGT GGAACAGACT 
CCAGAGGACG AGAACCAAAC ACCTCCAGTG ACCTTCCTGG AGTTTCAGTG GGGTCCGAGC 
CAAGACCTGC AGCAGGTCTA CCAGCAGAAA CGACAAGCAC TGCTCCAGAG ATCATCGCAC 
AGGGTCGAGG AAATCAAGTC CAGAAGAGAT GGTCCAAAGG TTCAGCCTGA GGTCCAAGCT 
CCACCCAGAG AGCAGTCTAA AGCTAAAGAC TCTCAACCTG TCAGCTGTAA ATCAAAGACT 
AAGACTGAAC CTCAGCCTAA GACGAACACA AAGTCAGAGG GAGGACGGGC TGAGCAACAG 
ACGTCTGACA AGTCTGGTTT CAAGCAGAAA AAGGTCCAGT GTCGACCTTC AGTGAGCGAC 
TGCAGGCTGA AGATCTGCAC ACCGGAGCAG AGGAAGCTGG ATGTTACTGA GATGCATCAG 
AGGACTCAGA GACTGTACGA GCAGCTGGAG GAGGTGAAGC ATCAGAAAGC AGCGAGGAGC 
CGACAGGAGG CGTCTGCAGA GAACAGGCTG AAGGCCAAAG AGTTCCACAA GAAAACGTTA 
CAGAAACTTC GTGCCAAGAC GACTCGGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027135701.1
CDS	283..6015
Translation	MKRKVSQLRLSPNEEARLIREEHERRRKLRIQQVREQQRYIALQIRREVELRRRRELEQLKEELRDDWERQQREKLNTLQKLYQESLQLLGQGHRSAKENEPDLAAIAQREEENHSKAEERYREALNELKSQRLKDQERQSRSINARKKALQAEKERAAKVASLPPPPPNPIQNIDSKKPHVVKKSDVSAFAATHYHMPESTVDREADPTQPNAHEEAELEARRMQDLQREEKQRREEQLEKARLRGKQALRREHLVQDRERLLLELEHMQQTDLLRRRQQVSHMPAQIFQPLYKRQEMREDFQREMEFAFEDMYTGERRVKGDLVVQLVPEPLPALSTGSQDQDQELDITLDEIPTPAAENTHHDTEQEAGSPEQEASARAETSRPAPRRALKKLLDRIRSQRTHWSEHNGHVPAADSPTAFTNQIPEHDTTIDTGSLTSEEKDKLPEPARSPPAVSETTEPSAEAETSAPVRLQEFTDERRKRELELEQEKQQQVVLIQELEEQKAKLEQMLFEAQQEREHLKAAVTQEVPVLDQEVTSVSSGLSSELVPPPGEDEHSRRIREYQQRLLEQNRIHQRSVEVARQRLEEYQRALQIRYSMTTKQSLPSVVPPGLVHPPLHSTGPVHLPAPPSLPTTPAVPAYVPARPHPSVEVTTREADMVASPPHLPGSSLRVCSRLLPDEVESVWNRAKNQKPDVSTWLTDNIMERVTEHLPDSVKPVSVTMKPPPHKLSTTRESTSILLQPTSHPIQVIGPSIADRTPLFPGHVAVKPHVSLLTGTLSSREDDMERQRRELQRQQEEEEEEERQRWELQEVQRRLLEQREAVEVQQRQKEEERQRQELQRRMQEQRAAVELQQKQQEEERQRRLLEQREAMEVQQRQTEEEKQRRELQEVQRRLLEQREAVELQKKQQEEERQRREVEMEEMRRQKETLQALIHTDAQPGAEVSSEVPLSESIGPTRRQLLASLLRAIQETNGGTLSHLEEPQEEDDSPQQPSSSYSVETDPVFQSSVPAGPLPASGLLPPPRAAKPPVTRVRLGVMKMMTEQHELSAIQEVETPVNSSLVTGLEDSVKLPSQTVGWDPQEESDSSVSSDRTLQTPSWSSSEQRPAERREASSRRYSHLDLLMEAGTSPESSESSDSVVRKTSRLSSDSGRGADYSGPAITSYRSFTESAQRPPDSDCLSSTTISSGSYITTDPEQNEPNVNTDKSLPIRRHVEPDGGANSLNVSSPSGQNFSMNSSTAARPALDVDSLFGDSSIQRIIDRYTRELNFSLSTTGRAADSEGSHVDDPGSVSQQSLVRVSERSRCLPSDAAAAQRSVQEWDNTVDPILEHFSVEGTEQDSFRPLIGRLADQSSCLAVDHRDSTMEQLVGQPSAHSSMIGQLPGLPVGLDQGGWDSTLSRMIGRLSHQSSPHWLSGGHDFYAGQMVSERSATWLDEESRMRPLVGELDESTGHHSGSSGERTRVDPDVSTEASVPSFPALPPEASSHSASDPAVNPHPQDQNQTSPMDLGPERTEDSFHLLVAEVTHNETAEPSVNFHAVPDSPEGRPASCEQSASAPPEEFETHNDPSIESELSPERLRTDKPPSCLTPPHALHESFSQLITSQHHPHEVELTALSLSNLTMCDDALTLKLQPEEPFRELESEQTVRPASEKLSEAGGEKGILEQSEITLVSLTDTTLQDQETTITDEDGVWDGKHPDRVMEEGQKETEESHSVEQTPEDENQTPPVTFLEFQWGPSQDLQQVYQQKRQALLQRSSHRVEEIKSRRDGPKVQPEVQAPPREQSKAKDSQPVSCKSKTKTEPQPKTNTKSEGGRAEQQTSDKSGFKQKKVQCRPSVSDCRLKICTPEQRKLDVTEMHQRTQRLYEQLEEVKHQKAARSRQEASAENRLKAKEFHKKTLQKLRAKTTRQ

Target ORF information:

RefSeq Version	XM_027279900.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027279900.1

ORF Insert Sequence:

ATGAAGAGGA AAGTTTCCCA GCTCAGACTG AGCCCCAACG AGGAGGCCAG GCTGATCCGA 
GAGGAGCATG AGAGGAGGAG GAAGCTGCGA ATACAGCAGG TGCGGGAGCA GCAGCGGTAC 
ATCGCGCTGC AGATCCGCCG AGAGGTCGAG TTGAGGCGGC GGCGTGAGCT CGAGCAGCTG 
AAGGAGGAGC TGAGGGACGA CTGGGAGCGA CAGCAGAGGG AGAAACTCAA CACACTGCAG 
AAACTTTACC AGGAGAGCCT CCAGCTTCTC GGCCAAGGAC ACAGGAGCGC AAAAGAAAAC 
GAACCCGACC TGGCAGCCAT AGCTCAGAGG GAGGAGGAAA ATCACAGCAA AGCAGAGGAG 
CGTTATCGAG AAGCTCTGAA TGAGCTCAAA TCACAGAGGC TTAAAGACCA AGAGAGGCAG 
AGCCGTTCTA TCAATGCCAG GAAGAAGGCG CTACAGGCAG AAAAGGAGAG AGCGGCAAAA 
GTGGCCAGCC TCCCGCCGCC GCCGCCAAAC CCCATTCAGA ACATCGATTC AAAGAAGCCT 
CATGTGGTAA AGAAGTCTGA TGTGAGCGCC TTTGCTGCTA CACATTACCA CATGCCTGAG 
AGCACAGTGG ACAGAGAGGC AGACCCAACA CAGCCTAACG CTCACGAGGA AGCTGAGCTG 
GAGGCGAGGA GAATGCAGGA CCTCCAGAGG GAGGAGAAGC AGAGGAGAGA GGAGCAGCTG 
GAGAAGGCTC GTCTCAGAGG GAAGCAGGCT CTGAGGAGGG AACACCTCGT GCAGGACCGT 
GAGCGCTTGC TCTTGGAGCT GGAACACATG CAGCAGACAG ACCTGCTGAG GAGGAGACAG 
CAGGTGTCAC ACATGCCTGC TCAGATCTTC CAGCCTCTCT ACAAGAGACA GGAGATGAGG 
GAGGACTTCC AGAGGGAGAT GGAGTTCGCC TTTGAGGACA TGTACACTGG AGAGAGGAGG 
GTTAAAGGTG ATCTGGTGGT CCAGCTGGTA CCGGAGCCTC TGCCAGCTCT GTCCACAGGC 
AGCCAAGACC AGGACCAGGA GCTGGACATC ACGCTGGACG AAATCCCCAC ACCAGCAGCA 
GAAAACACAC ACCATGACAC TGAGCAAGAA GCTGGAAGCC CTGAGCAGGA AGCATCTGCA 
CGAGCAGAGA CCTCCAGACC TGCTCCCAGA CGGGCGTTGA AGAAACTCCT GGATCGTATC 
AGGAGCCAGC GGACTCACTG GAGCGAACAC AACGGTCACG TCCCCGCAGC CGACTCGCCG 
ACCGCCTTCA CCAATCAGAT ACCAGAGCAC GACACAACCA TCGACACGGG ATCTCTGACC 
AGTGAGGAGA AGGACAAACT CCCTGAACCC GCCCGCTCTC CACCCGCAGT TTCAGAGACA 
ACAGAGCCGT CAGCTGAAGC AGAAACTTCG GCTCCTGTCC GACTCCAAGA ATTTACAGAC 
GAACGAAGGA AAAGGGAGTT GGAGCTCGAG CAGGAGAAGC AGCAGCAGGT GGTGTTGATC 
CAGGAGTTAG AGGAGCAGAA GGCCAAACTG GAGCAGATGC TGTTTGAAGC ACAGCAGGAG 
AGAGAGCACC TAAAGGCTGC TGTGACCCAG GAAGTGCCTG TCCTCGACCA GGAAGTCACC 
TCCGTCTCCT CCGGGCTGTC CAGTGAGCTG GTGCCTCCTC CGGGTGAAGA TGAACACAGC 
AGAAGGATTC GAGAATACCA ACAGAGGCTG CTGGAACAGA ACAGAATTCA TCAGAGGTCG 
GTGGAGGTGG CTCGTCAGCG GCTGGAGGAA TACCAGCGAG CTCTACAGAT CCGTTACAGC 
ATGACCACCA AACAGTCGCT GCCCTCCGTC GTGCCTCCAG GACTCGTTCA CCCGCCGCTA 
CACAGTACTG GGCCTGTTCA TCTTCCTGCT CCTCCGTCAC TCCCAACAAC TCCTGCAGTG 
CCTGCATACG TCCCTGCCAG ACCACACCCC TCTGTGGAAG TCACTACAAG AGAGGCTGAC 
ATGGTGGCTT CACCTCCCCA CCTTCCTGGC TCCAGTTTGA GGGTTTGCTC CAGGTTGCTG 
CCAGATGAAG TGGAATCTGT TTGGAACAGA GCGAAGAACC AGAAACCCGA TGTTTCTACC 
TGGCTGACTG ACAACATAAT GGAGAGAGTG ACTGAGCACC TTCCAGACAG CGTGAAACCC 
GTCTCAGTCA CCATGAAGCC ACCGCCCCAC AAACTGTCCA CAACACGTGA GTCTACCAGC 
ATCCTGCTTC AGCCGACCTC TCATCCCATC CAAGTCATCG GCCCCAGCAT TGCAGATAGA 
ACACCTCTGT TTCCAGGCCA TGTAGCGGTT AAGCCGCATG TCTCCCTGCT GACTGGGACC 
CTGAGCTCCA GAGAGGACGA CATGGAGAGG CAGAGACGGG AGCTGCAGAG GCAACAAGAG 
GAGGAGGAGG AGGAGGAGAG GCAGAGATGG GAGCTGCAGG AGGTTCAGAG ACGATTGCTG 
GAGCAGAGGG AGGCAGTGGA AGTGCAACAG AGGCAGAAAG AAGAGGAGAG GCAGAGACAG 
GAGCTGCAGA GACGAATGCA GGAGCAAAGA GCGGCAGTAG AGCTCCAGCA GAAGCAGCAA 
GAAGAGGAGA GGCAGAGACG GTTGCTGGAG CAGAGGGAGG CGATGGAGGT GCAACAAAGG 
CAGACAGAGG AGGAGAAGCA GAGGCGGGAG CTGCAGGAGG TTCAGAGACG GTTGTTGGAG 
CAGAGGGAGG CAGTGGAGCT GCAGAAGAAG CAACAAGAAG AGGAGAGGCA GAGGCGGGAA 
GTGGAGATGG AGGAGATGAG GCGACAGAAG GAGACGTTAC AGGCTCTGAT TCATACTGAC 
GCACAGCCAG GTGCAGAGGT CTCCAGTGAA GTGCCGCTTT CAGAGAGCAT CGGTCCAACT 
CGACGTCAGT TACTTGCATC ACTGCTGCGA GCCATACAGG AAACTAACGG AGGAACTCTA 
TCACATCTAG AAGAGCCGCA GGAGGAAGAT GACTCTCCTC AACAACCATC ATCATCTTAC 
AGCGTCGAGA CTGACCCCGT CTTTCAGAGC AGTGTTCCTG CTGGACCTCT CCCAGCATCA 
GGCCTCCTGC CTCCTCCTCG AGCAGCGAAG CCTCCAGTGA CTCGCGTCAG GCTGGGCGTC 
ATGAAGATGA TGACTGAACA ACATGAGCTC AGTGCTATTC AAGAGGTGGA GACGCCAGTC 
AACAGCAGCC TGGTCACAGG TCTGGAGGAC AGTGTGAAGC TTCCTTCACA GACTGTGGGC 
TGGGATCCAC AGGAGGAGTC CGACTCGTCG GTGTCCTCTG ACAGGACTCT GCAGACGCCC 
TCTTGGTCCA GCAGCGAGCA GCGGCCTGCT GAAAGACGAG AGGCGAGCTC GAGGAGGTAC 
AGCCATCTGG ATCTGCTGAT GGAGGCTGGA ACATCTCCAG AATCCTCAGA GTCATCTGAT 
TCAGTCGTGA GGAAAACGTC ACGGCTTTCC TCTGACTCTG GCAGAGGAGC TGACTACTCC 
GGACCTGCGA TCACGAGCTA CAGATCCTTC ACAGAGTCTG CACAGAGGCC TCCTGACTCT 
GACTGCCTCT CCTCCACCAC CATCTCCAGC GGCAGCTACA TCACCACAGA CCCTGAACAA 
AACGAGCCAA ACGTAAACAC TGATAAATCT CTGCCCATCA GACGTCATGT GGAACCAGAT 
GGAGGAGCAA ATTCGCTGAA CGTCTCCTCT CCGTCGGGTC AAAACTTCTC CATGAACAGC 
TCGACTGCAG CCCGTCCTGC TCTGGATGTC GACTCTCTGT TTGGTGACAG CAGCATCCAG 
CGAATCATCG ACCGATACAC GAGAGAGCTC AACTTCTCCC TCAGCACCAC CGGGAGAGCC 
GCAGACAGTG AAGGCTCTCA TGTGGACGAT CCCGGCTCAG TCTCTCAGCA GTCTTTGGTT 
CGAGTCTCAG AGAGGAGTCG GTGTCTTCCC TCAGACGCAG CAGCAGCACA GAGGAGTGTC 
CAGGAATGGG ACAACACAGT CGATCCAATC CTGGAGCACT TCTCAGTTGA GGGCACAGAG 
CAGGACTCTT TCAGACCTCT GATTGGCCGG CTGGCAGACC AATCATCCTG CTTGGCTGTC 
GACCACAGGG ACTCGACCAT GGAGCAGCTG GTCGGTCAGC CATCGGCTCA CTCGTCCATG 
ATCGGTCAGC TGCCCGGGCT GCCGGTGGGC CTCGATCAAG GCGGATGGGA TTCCACTCTG 
AGCCGGATGA TTGGTCGACT CTCCCATCAG TCCAGCCCTC ATTGGCTGAG TGGAGGCCAC 
GACTTCTATG CGGGTCAGAT GGTGTCAGAG CGATCAGCCA CGTGGTTGGA TGAAGAGAGC 
CGGATGAGAC CGCTGGTCGG AGAGCTGGAT GAGTCCACTG GACACCACAG TGGGAGCTCA 
GGTGAAAGAA CTCGCGTGGA TCCCGATGTT TCCACTGAGG CCAGTGTGCC GTCATTCCCA 
GCGTTGCCTC CTGAAGCGTC GTCACACAGC GCCTCTGACC CGGCTGTGAA TCCACACCCC 
CAGGACCAGA ACCAAACCAG TCCAATGGAC CTGGGCCCAG AGCGGACTGA AGACTCGTTC 
CACCTGCTCG TGGCTGAAGT CACCCACAAC GAAACAGCCG AGCCCTCCGT CAACTTCCAC 
GCTGTGCCTG ACTCTCCTGA AGGTCGGCCA GCCAGCTGTG AACAGAGCGC CTCTGCACCG 
CCTGAAGAGT TTGAAACCCA CAACGACCCG TCCATCGAGT CTGAACTGTC TCCTGAGCGC 
CTCAGGACAG ACAAGCCGCC CAGTTGCCTG ACGCCGCCCC ACGCCTTACA TGAGTCTTTC 
TCCCAGCTCA TCACCTCCCA GCACCACCCC CATGAGGTGG AGCTAACCGC TCTGAGTCTG 
TCAAATCTCA CCATGTGTGA TGATGCGCTG ACTCTGAAGC TGCAGCCTGA GGAACCGTTC 
AGAGAGCTGG AGTCTGAGCA GACTGTTCGT CCTGCCTCAG AGAAGCTGTC GGAGGCTGGC 
GGTGAGAAAG GCATCCTGGA GCAGTCGGAG ATAACACTCG TGAGCCTGAC AGACACAACT 
CTGCAGGACC AAGAGACGAC CATCACCGAC GAAGACGGAG TCTGGGACGG CAAACATCCA 
GACAGGGTGA TGGAGGAAGG ACAGAAAGAG ACAGAGGAGT CGCACTCAGT GGAACAGACT 
CCAGAGGACG AGAACCAAAC ACCTCCAGTG ACCTTCCTGG AGTTTCAGTG GGGTCCGAGC 
CAAGACCTGC AGCAGGTCTA CCAGCAGAAA CGACAAGCAC TGCTCCAGAG ATCATCGCAC 
AGGGTCGAGG AAATCAAGTC CAGAAGAGAT GGTCCAAAGG TTCAGCCTGA GGTCCAAGCT 
CCACCCAGAG AGCAGTCTAA AGCTAAAGAC TCTCAACCTG TCAGCTGTAA ATCAAAGACT 
AAGACTGAAC CTCAGCCTAA GACGAACACA AAGTCAGAGG GAGGACGGGC TGAGCAACAG 
ACGTCTGACA AGTCTGGTTT CAAGCAGAAA AAGGTCCAGT GTCGACCTTC AGTGAGCGAC 
TGCAGGCTGA AGATCTGCAC ACCGGAGCAG AGGAAGCTGG ATGTTACTGA GATGCATCAG 
AGGACTCAGA GACTGTACGA GCAGCTGGAG GAGGTGAAGC ATCAGAAAGC AGCGAGGAGC 
CGACAGGAGG CGTCTGCAGA GAACAGGCTG AAGGCCAAAG AGTTCCACAA GAAAACGTTA 
CAGAAACTTC GTGCCAAGAC GACTCGGCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep295 ( XM_027279901.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027135702.1 Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X2, mRNA.

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CloneID	OLa221269
Clone ID Related Accession (Same CDS sequence)	XM_027279901.1
Accession Version	XM_027279901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	centrosomal protein of 295 kDa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040017.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA AAGTTTCCCA GCTCAGACTG AGCCCCAACG AGGAGGCCAG GCTGATCCGA 
GAGGAGCATG AGAGGAGGAG GAAGCTGCGA ATACAGCAGG TGCGGGAGCA GCAGCGGTAC 
ATCGCGCTGC AGATCCGCCG AGAGGTCGAG TTGAGGCGGC GGCGTGAGCT CGAGCAGCTG 
AAGGAGGAGC TGAGGGACGA CTGGGAGCGA CAGCAGAGGG AGAAACTCAA CACACTGCAG 
AAACTTTACC AGGAGAGCCT CCAGCTTCTC GGCCAAGGAC ACAGGAGCGC AAAAGAAAAC 
GAACCCGACC TGGCAGCCAT AGCTCAGAGG GAGGAGGAAA ATCACAGCAA AGCAGAGGAG 
CGTTATCGAG AAGCTCTGAA TGAGCTCAAA TCACAGAGGC TTAAAGACCA AGAGAGGCAG 
AGCCGTTCTA TCAATGCCAG GAAGAAGGCG CTACAGGCAG AAAAGGAGAG AGCGGCAAAA 
GTGGCCAGCC TCCCGCCGCC GCCGCCAAAC CCCATTCAGA ACATCGATTC AAAGAAGCCT 
CATGTGGTAA AGAAGTCTGA TGTGAGCGCC TTTGCTGCTA CACATTACCA CATGCCTGAG 
AGCACAGTGG ACAGAGAGGC AGACCCAACA CAGCCTAACG CTCACGAGGA AGCTGAGCTG 
GAGGCGAGGA GAATGCAGGA CCTCCAGAGG GAGGAGAAGC AGAGGAGAGA GGAGCAGCTG 
GAGAAGGCTC GTCTCAGAGG GAAGCAGGCT CTGAGGAGGG AACACCTCGT GCAGGACCGT 
GAGCGCTTGC TCTTGGAGCT GGAACACATG CAGCAGACAG ACCTGCTGAG GAGGAGACAG 
CAGGTGTCAC ACATGCCTGC TCAGATCTTC CAGCCTCTCT ACAAGAGACA GGAGATGAGG 
GAGGACTTCC AGAGGGAGAT GGAGTTCGCC TTTGAGGACA TGTACACTGG AGAGAGGAGG 
GTTAAAGGTG ATCTGGTGGT CCAGCTGGTA CCGGAGCCTC TGCCAGCTCT GTCCACAGGC 
AGCCAAGACC AGGACCAGGA GCTGGACATC ACGCTGGACG AAATCCCCAC ACCAGCAGCA 
GAAAACACAC ACCATGACAC TGAGCAAGAA GCTGGAAGCC CTGAGCAGGA AGCATCTGCA 
CGAGAGACCT CCAGACCTGC TCCCAGACGG GCGTTGAAGA AACTCCTGGA TCGTATCAGG 
AGCCAGCGGA CTCACTGGAG CGAACACAAC GGTCACGTCC CCGCAGCCGA CTCGCCGACC 
GCCTTCACCA ATCAGATACC AGAGCACGAC ACAACCATCG ACACGGGATC TCTGACCAGT 
GAGGAGAAGG ACAAACTCCC TGAACCCGCC CGCTCTCCAC CCGCAGTTTC AGAGACAACA 
GAGCCGTCAG CTGAAGCAGA AACTTCGGCT CCTGTCCGAC TCCAAGAATT TACAGACGAA 
CGAAGGAAAA GGGAGTTGGA GCTCGAGCAG GAGAAGCAGC AGCAGGTGGT GTTGATCCAG 
GAGTTAGAGG AGCAGAAGGC CAAACTGGAG CAGATGCTGT TTGAAGCACA GCAGGAGAGA 
GAGCACCTAA AGGCTGCTGT GACCCAGGAA GTGCCTGTCC TCGACCAGGA AGTCACCTCC 
GTCTCCTCCG GGCTGTCCAG TGAGCTGGTG CCTCCTCCGG GTGAAGATGA ACACAGCAGA 
AGGATTCGAG AATACCAACA GAGGCTGCTG GAACAGAACA GAATTCATCA GAGGTCGGTG 
GAGGTGGCTC GTCAGCGGCT GGAGGAATAC CAGCGAGCTC TACAGATCCG TTACAGCATG 
ACCACCAAAC AGTCGCTGCC CTCCGTCGTG CCTCCAGGAC TCGTTCACCC GCCGCTACAC 
AGTACTGGGC CTGTTCATCT TCCTGCTCCT CCGTCACTCC CAACAACTCC TGCAGTGCCT 
GCATACGTCC CTGCCAGACC ACACCCCTCT GTGGAAGTCA CTACAAGAGA GGCTGACATG 
GTGGCTTCAC CTCCCCACCT TCCTGGCTCC AGTTTGAGGG TTTGCTCCAG GTTGCTGCCA 
GATGAAGTGG AATCTGTTTG GAACAGAGCG AAGAACCAGA AACCCGATGT TTCTACCTGG 
CTGACTGACA ACATAATGGA GAGAGTGACT GAGCACCTTC CAGACAGCGT GAAACCCGTC 
TCAGTCACCA TGAAGCCACC GCCCCACAAA CTGTCCACAA CACGTGAGTC TACCAGCATC 
CTGCTTCAGC CGACCTCTCA TCCCATCCAA GTCATCGGCC CCAGCATTGC AGATAGAACA 
CCTCTGTTTC CAGGCCATGT AGCGGTTAAG CCGCATGTCT CCCTGCTGAC TGGGACCCTG 
AGCTCCAGAG AGGACGACAT GGAGAGGCAG AGACGGGAGC TGCAGAGGCA ACAAGAGGAG 
GAGGAGGAGG AGGAGAGGCA GAGATGGGAG CTGCAGGAGG TTCAGAGACG ATTGCTGGAG 
CAGAGGGAGG CAGTGGAAGT GCAACAGAGG CAGAAAGAAG AGGAGAGGCA GAGACAGGAG 
CTGCAGAGAC GAATGCAGGA GCAAAGAGCG GCAGTAGAGC TCCAGCAGAA GCAGCAAGAA 
GAGGAGAGGC AGAGACGGTT GCTGGAGCAG AGGGAGGCGA TGGAGGTGCA ACAAAGGCAG 
ACAGAGGAGG AGAAGCAGAG GCGGGAGCTG CAGGAGGTTC AGAGACGGTT GTTGGAGCAG 
AGGGAGGCAG TGGAGCTGCA GAAGAAGCAA CAAGAAGAGG AGAGGCAGAG GCGGGAAGTG 
GAGATGGAGG AGATGAGGCG ACAGAAGGAG ACGTTACAGG CTCTGATTCA TACTGACGCA 
CAGCCAGGTG CAGAGGTCTC CAGTGAAGTG CCGCTTTCAG AGAGCATCGG TCCAACTCGA 
CGTCAGTTAC TTGCATCACT GCTGCGAGCC ATACAGGAAA CTAACGGAGG AACTCTATCA 
CATCTAGAAG AGCCGCAGGA GGAAGATGAC TCTCCTCAAC AACCATCATC ATCTTACAGC 
GTCGAGACTG ACCCCGTCTT TCAGAGCAGT GTTCCTGCTG GACCTCTCCC AGCATCAGGC 
CTCCTGCCTC CTCCTCGAGC AGCGAAGCCT CCAGTGACTC GCGTCAGGCT GGGCGTCATG 
AAGATGATGA CTGAACAACA TGAGCTCAGT GCTATTCAAG AGGTGGAGAC GCCAGTCAAC 
AGCAGCCTGG TCACAGGTCT GGAGGACAGT GTGAAGCTTC CTTCACAGAC TGTGGGCTGG 
GATCCACAGG AGGAGTCCGA CTCGTCGGTG TCCTCTGACA GGACTCTGCA GACGCCCTCT 
TGGTCCAGCA GCGAGCAGCG GCCTGCTGAA AGACGAGAGG CGAGCTCGAG GAGGTACAGC 
CATCTGGATC TGCTGATGGA GGCTGGAACA TCTCCAGAAT CCTCAGAGTC ATCTGATTCA 
GTCGTGAGGA AAACGTCACG GCTTTCCTCT GACTCTGGCA GAGGAGCTGA CTACTCCGGA 
CCTGCGATCA CGAGCTACAG ATCCTTCACA GAGTCTGCAC AGAGGCCTCC TGACTCTGAC 
TGCCTCTCCT CCACCACCAT CTCCAGCGGC AGCTACATCA CCACAGACCC TGAACAAAAC 
GAGCCAAACG TAAACACTGA TAAATCTCTG CCCATCAGAC GTCATGTGGA ACCAGATGGA 
GGAGCAAATT CGCTGAACGT CTCCTCTCCG TCGGGTCAAA ACTTCTCCAT GAACAGCTCG 
ACTGCAGCCC GTCCTGCTCT GGATGTCGAC TCTCTGTTTG GTGACAGCAG CATCCAGCGA 
ATCATCGACC GATACACGAG AGAGCTCAAC TTCTCCCTCA GCACCACCGG GAGAGCCGCA 
GACAGTGAAG GCTCTCATGT GGACGATCCC GGCTCAGTCT CTCAGCAGTC TTTGGTTCGA 
GTCTCAGAGA GGAGTCGGTG TCTTCCCTCA GACGCAGCAG CAGCACAGAG GAGTGTCCAG 
GAATGGGACA ACACAGTCGA TCCAATCCTG GAGCACTTCT CAGTTGAGGG CACAGAGCAG 
GACTCTTTCA GACCTCTGAT TGGCCGGCTG GCAGACCAAT CATCCTGCTT GGCTGTCGAC 
CACAGGGACT CGACCATGGA GCAGCTGGTC GGTCAGCCAT CGGCTCACTC GTCCATGATC 
GGTCAGCTGC CCGGGCTGCC GGTGGGCCTC GATCAAGGCG GATGGGATTC CACTCTGAGC 
CGGATGATTG GTCGACTCTC CCATCAGTCC AGCCCTCATT GGCTGAGTGG AGGCCACGAC 
TTCTATGCGG GTCAGATGGT GTCAGAGCGA TCAGCCACGT GGTTGGATGA AGAGAGCCGG 
ATGAGACCGC TGGTCGGAGA GCTGGATGAG TCCACTGGAC ACCACAGTGG GAGCTCAGGT 
GAAAGAACTC GCGTGGATCC CGATGTTTCC ACTGAGGCCA GTGTGCCGTC ATTCCCAGCG 
TTGCCTCCTG AAGCGTCGTC ACACAGCGCC TCTGACCCGG CTGTGAATCC ACACCCCCAG 
GACCAGAACC AAACCAGTCC AATGGACCTG GGCCCAGAGC GGACTGAAGA CTCGTTCCAC 
CTGCTCGTGG CTGAAGTCAC CCACAACGAA ACAGCCGAGC CCTCCGTCAA CTTCCACGCT 
GTGCCTGACT CTCCTGAAGG TCGGCCAGCC AGCTGTGAAC AGAGCGCCTC TGCACCGCCT 
GAAGAGTTTG AAACCCACAA CGACCCGTCC ATCGAGTCTG AACTGTCTCC TGAGCGCCTC 
AGGACAGACA AGCCGCCCAG TTGCCTGACG CCGCCCCACG CCTTACATGA GTCTTTCTCC 
CAGCTCATCA CCTCCCAGCA CCACCCCCAT GAGGTGGAGC TAACCGCTCT GAGTCTGTCA 
AATCTCACCA TGTGTGATGA TGCGCTGACT CTGAAGCTGC AGCCTGAGGA ACCGTTCAGA 
GAGCTGGAGT CTGAGCAGAC TGTTCGTCCT GCCTCAGAGA AGCTGTCGGA GGCTGGCGGT 
GAGAAAGGCA TCCTGGAGCA GTCGGAGATA ACACTCGTGA GCCTGACAGA CACAACTCTG 
CAGGACCAAG AGACGACCAT CACCGACGAA GACGGAGTCT GGGACGGCAA ACATCCAGAC 
AGGGTGATGG AGGAAGGACA GAAAGAGACA GAGGAGTCGC ACTCAGTGGA ACAGACTCCA 
GAGGACGAGA ACCAAACACC TCCAGTGACC TTCCTGGAGT TTCAGTGGGG TCCGAGCCAA 
GACCTGCAGC AGGTCTACCA GCAGAAACGA CAAGCACTGC TCCAGAGATC ATCGCACAGG 
GTCGAGGAAA TCAAGTCCAG AAGAGATGGT CCAAAGGTTC AGCCTGAGGT CCAAGCTCCA 
CCCAGAGAGC AGTCTAAAGC TAAAGACTCT CAACCTGTCA GCTGTAAATC AAAGACTAAG 
ACTGAACCTC AGCCTAAGAC GAACACAAAG TCAGAGGGAG GACGGGCTGA GCAACAGACG 
TCTGACAAGT CTGGTTTCAA GCAGAAAAAG GTCCAGTGTC GACCTTCAGT GAGCGACTGC 
AGGCTGAAGA TCTGCACACC GGAGCAGAGG AAGCTGGATG TTACTGAGAT GCATCAGAGG 
ACTCAGAGAC TGTACGAGCA GCTGGAGGAG GTGAAGCATC AGAAAGCAGC GAGGAGCCGA 
CAGGAGGCGT CTGCAGAGAA CAGGCTGAAG GCCAAAGAGT TCCACAAGAA AACGTTACAG 
AAACTTCGTG CCAAGACGAC TCGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027135702.1
CDS	283..6012
Translation	MKRKVSQLRLSPNEEARLIREEHERRRKLRIQQVREQQRYIALQIRREVELRRRRELEQLKEELRDDWERQQREKLNTLQKLYQESLQLLGQGHRSAKENEPDLAAIAQREEENHSKAEERYREALNELKSQRLKDQERQSRSINARKKALQAEKERAAKVASLPPPPPNPIQNIDSKKPHVVKKSDVSAFAATHYHMPESTVDREADPTQPNAHEEAELEARRMQDLQREEKQRREEQLEKARLRGKQALRREHLVQDRERLLLELEHMQQTDLLRRRQQVSHMPAQIFQPLYKRQEMREDFQREMEFAFEDMYTGERRVKGDLVVQLVPEPLPALSTGSQDQDQELDITLDEIPTPAAENTHHDTEQEAGSPEQEASARETSRPAPRRALKKLLDRIRSQRTHWSEHNGHVPAADSPTAFTNQIPEHDTTIDTGSLTSEEKDKLPEPARSPPAVSETTEPSAEAETSAPVRLQEFTDERRKRELELEQEKQQQVVLIQELEEQKAKLEQMLFEAQQEREHLKAAVTQEVPVLDQEVTSVSSGLSSELVPPPGEDEHSRRIREYQQRLLEQNRIHQRSVEVARQRLEEYQRALQIRYSMTTKQSLPSVVPPGLVHPPLHSTGPVHLPAPPSLPTTPAVPAYVPARPHPSVEVTTREADMVASPPHLPGSSLRVCSRLLPDEVESVWNRAKNQKPDVSTWLTDNIMERVTEHLPDSVKPVSVTMKPPPHKLSTTRESTSILLQPTSHPIQVIGPSIADRTPLFPGHVAVKPHVSLLTGTLSSREDDMERQRRELQRQQEEEEEEERQRWELQEVQRRLLEQREAVEVQQRQKEEERQRQELQRRMQEQRAAVELQQKQQEEERQRRLLEQREAMEVQQRQTEEEKQRRELQEVQRRLLEQREAVELQKKQQEEERQRREVEMEEMRRQKETLQALIHTDAQPGAEVSSEVPLSESIGPTRRQLLASLLRAIQETNGGTLSHLEEPQEEDDSPQQPSSSYSVETDPVFQSSVPAGPLPASGLLPPPRAAKPPVTRVRLGVMKMMTEQHELSAIQEVETPVNSSLVTGLEDSVKLPSQTVGWDPQEESDSSVSSDRTLQTPSWSSSEQRPAERREASSRRYSHLDLLMEAGTSPESSESSDSVVRKTSRLSSDSGRGADYSGPAITSYRSFTESAQRPPDSDCLSSTTISSGSYITTDPEQNEPNVNTDKSLPIRRHVEPDGGANSLNVSSPSGQNFSMNSSTAARPALDVDSLFGDSSIQRIIDRYTRELNFSLSTTGRAADSEGSHVDDPGSVSQQSLVRVSERSRCLPSDAAAAQRSVQEWDNTVDPILEHFSVEGTEQDSFRPLIGRLADQSSCLAVDHRDSTMEQLVGQPSAHSSMIGQLPGLPVGLDQGGWDSTLSRMIGRLSHQSSPHWLSGGHDFYAGQMVSERSATWLDEESRMRPLVGELDESTGHHSGSSGERTRVDPDVSTEASVPSFPALPPEASSHSASDPAVNPHPQDQNQTSPMDLGPERTEDSFHLLVAEVTHNETAEPSVNFHAVPDSPEGRPASCEQSASAPPEEFETHNDPSIESELSPERLRTDKPPSCLTPPHALHESFSQLITSQHHPHEVELTALSLSNLTMCDDALTLKLQPEEPFRELESEQTVRPASEKLSEAGGEKGILEQSEITLVSLTDTTLQDQETTITDEDGVWDGKHPDRVMEEGQKETEESHSVEQTPEDENQTPPVTFLEFQWGPSQDLQQVYQQKRQALLQRSSHRVEEIKSRRDGPKVQPEVQAPPREQSKAKDSQPVSCKSKTKTEPQPKTNTKSEGGRAEQQTSDKSGFKQKKVQCRPSVSDCRLKICTPEQRKLDVTEMHQRTQRLYEQLEEVKHQKAARSRQEASAENRLKAKEFHKKTLQKLRAKTTRQ

Target ORF information:

RefSeq Version	XM_027279901.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027279901.1

ORF Insert Sequence:

ATGAAGAGGA AAGTTTCCCA GCTCAGACTG AGCCCCAACG AGGAGGCCAG GCTGATCCGA 
GAGGAGCATG AGAGGAGGAG GAAGCTGCGA ATACAGCAGG TGCGGGAGCA GCAGCGGTAC 
ATCGCGCTGC AGATCCGCCG AGAGGTCGAG TTGAGGCGGC GGCGTGAGCT CGAGCAGCTG 
AAGGAGGAGC TGAGGGACGA CTGGGAGCGA CAGCAGAGGG AGAAACTCAA CACACTGCAG 
AAACTTTACC AGGAGAGCCT CCAGCTTCTC GGCCAAGGAC ACAGGAGCGC AAAAGAAAAC 
GAACCCGACC TGGCAGCCAT AGCTCAGAGG GAGGAGGAAA ATCACAGCAA AGCAGAGGAG 
CGTTATCGAG AAGCTCTGAA TGAGCTCAAA TCACAGAGGC TTAAAGACCA AGAGAGGCAG 
AGCCGTTCTA TCAATGCCAG GAAGAAGGCG CTACAGGCAG AAAAGGAGAG AGCGGCAAAA 
GTGGCCAGCC TCCCGCCGCC GCCGCCAAAC CCCATTCAGA ACATCGATTC AAAGAAGCCT 
CATGTGGTAA AGAAGTCTGA TGTGAGCGCC TTTGCTGCTA CACATTACCA CATGCCTGAG 
AGCACAGTGG ACAGAGAGGC AGACCCAACA CAGCCTAACG CTCACGAGGA AGCTGAGCTG 
GAGGCGAGGA GAATGCAGGA CCTCCAGAGG GAGGAGAAGC AGAGGAGAGA GGAGCAGCTG 
GAGAAGGCTC GTCTCAGAGG GAAGCAGGCT CTGAGGAGGG AACACCTCGT GCAGGACCGT 
GAGCGCTTGC TCTTGGAGCT GGAACACATG CAGCAGACAG ACCTGCTGAG GAGGAGACAG 
CAGGTGTCAC ACATGCCTGC TCAGATCTTC CAGCCTCTCT ACAAGAGACA GGAGATGAGG 
GAGGACTTCC AGAGGGAGAT GGAGTTCGCC TTTGAGGACA TGTACACTGG AGAGAGGAGG 
GTTAAAGGTG ATCTGGTGGT CCAGCTGGTA CCGGAGCCTC TGCCAGCTCT GTCCACAGGC 
AGCCAAGACC AGGACCAGGA GCTGGACATC ACGCTGGACG AAATCCCCAC ACCAGCAGCA 
GAAAACACAC ACCATGACAC TGAGCAAGAA GCTGGAAGCC CTGAGCAGGA AGCATCTGCA 
CGAGAGACCT CCAGACCTGC TCCCAGACGG GCGTTGAAGA AACTCCTGGA TCGTATCAGG 
AGCCAGCGGA CTCACTGGAG CGAACACAAC GGTCACGTCC CCGCAGCCGA CTCGCCGACC 
GCCTTCACCA ATCAGATACC AGAGCACGAC ACAACCATCG ACACGGGATC TCTGACCAGT 
GAGGAGAAGG ACAAACTCCC TGAACCCGCC CGCTCTCCAC CCGCAGTTTC AGAGACAACA 
GAGCCGTCAG CTGAAGCAGA AACTTCGGCT CCTGTCCGAC TCCAAGAATT TACAGACGAA 
CGAAGGAAAA GGGAGTTGGA GCTCGAGCAG GAGAAGCAGC AGCAGGTGGT GTTGATCCAG 
GAGTTAGAGG AGCAGAAGGC CAAACTGGAG CAGATGCTGT TTGAAGCACA GCAGGAGAGA 
GAGCACCTAA AGGCTGCTGT GACCCAGGAA GTGCCTGTCC TCGACCAGGA AGTCACCTCC 
GTCTCCTCCG GGCTGTCCAG TGAGCTGGTG CCTCCTCCGG GTGAAGATGA ACACAGCAGA 
AGGATTCGAG AATACCAACA GAGGCTGCTG GAACAGAACA GAATTCATCA GAGGTCGGTG 
GAGGTGGCTC GTCAGCGGCT GGAGGAATAC CAGCGAGCTC TACAGATCCG TTACAGCATG 
ACCACCAAAC AGTCGCTGCC CTCCGTCGTG CCTCCAGGAC TCGTTCACCC GCCGCTACAC 
AGTACTGGGC CTGTTCATCT TCCTGCTCCT CCGTCACTCC CAACAACTCC TGCAGTGCCT 
GCATACGTCC CTGCCAGACC ACACCCCTCT GTGGAAGTCA CTACAAGAGA GGCTGACATG 
GTGGCTTCAC CTCCCCACCT TCCTGGCTCC AGTTTGAGGG TTTGCTCCAG GTTGCTGCCA 
GATGAAGTGG AATCTGTTTG GAACAGAGCG AAGAACCAGA AACCCGATGT TTCTACCTGG 
CTGACTGACA ACATAATGGA GAGAGTGACT GAGCACCTTC CAGACAGCGT GAAACCCGTC 
TCAGTCACCA TGAAGCCACC GCCCCACAAA CTGTCCACAA CACGTGAGTC TACCAGCATC 
CTGCTTCAGC CGACCTCTCA TCCCATCCAA GTCATCGGCC CCAGCATTGC AGATAGAACA 
CCTCTGTTTC CAGGCCATGT AGCGGTTAAG CCGCATGTCT CCCTGCTGAC TGGGACCCTG 
AGCTCCAGAG AGGACGACAT GGAGAGGCAG AGACGGGAGC TGCAGAGGCA ACAAGAGGAG 
GAGGAGGAGG AGGAGAGGCA GAGATGGGAG CTGCAGGAGG TTCAGAGACG ATTGCTGGAG 
CAGAGGGAGG CAGTGGAAGT GCAACAGAGG CAGAAAGAAG AGGAGAGGCA GAGACAGGAG 
CTGCAGAGAC GAATGCAGGA GCAAAGAGCG GCAGTAGAGC TCCAGCAGAA GCAGCAAGAA 
GAGGAGAGGC AGAGACGGTT GCTGGAGCAG AGGGAGGCGA TGGAGGTGCA ACAAAGGCAG 
ACAGAGGAGG AGAAGCAGAG GCGGGAGCTG CAGGAGGTTC AGAGACGGTT GTTGGAGCAG 
AGGGAGGCAG TGGAGCTGCA GAAGAAGCAA CAAGAAGAGG AGAGGCAGAG GCGGGAAGTG 
GAGATGGAGG AGATGAGGCG ACAGAAGGAG ACGTTACAGG CTCTGATTCA TACTGACGCA 
CAGCCAGGTG CAGAGGTCTC CAGTGAAGTG CCGCTTTCAG AGAGCATCGG TCCAACTCGA 
CGTCAGTTAC TTGCATCACT GCTGCGAGCC ATACAGGAAA CTAACGGAGG AACTCTATCA 
CATCTAGAAG AGCCGCAGGA GGAAGATGAC TCTCCTCAAC AACCATCATC ATCTTACAGC 
GTCGAGACTG ACCCCGTCTT TCAGAGCAGT GTTCCTGCTG GACCTCTCCC AGCATCAGGC 
CTCCTGCCTC CTCCTCGAGC AGCGAAGCCT CCAGTGACTC GCGTCAGGCT GGGCGTCATG 
AAGATGATGA CTGAACAACA TGAGCTCAGT GCTATTCAAG AGGTGGAGAC GCCAGTCAAC 
AGCAGCCTGG TCACAGGTCT GGAGGACAGT GTGAAGCTTC CTTCACAGAC TGTGGGCTGG 
GATCCACAGG AGGAGTCCGA CTCGTCGGTG TCCTCTGACA GGACTCTGCA GACGCCCTCT 
TGGTCCAGCA GCGAGCAGCG GCCTGCTGAA AGACGAGAGG CGAGCTCGAG GAGGTACAGC 
CATCTGGATC TGCTGATGGA GGCTGGAACA TCTCCAGAAT CCTCAGAGTC ATCTGATTCA 
GTCGTGAGGA AAACGTCACG GCTTTCCTCT GACTCTGGCA GAGGAGCTGA CTACTCCGGA 
CCTGCGATCA CGAGCTACAG ATCCTTCACA GAGTCTGCAC AGAGGCCTCC TGACTCTGAC 
TGCCTCTCCT CCACCACCAT CTCCAGCGGC AGCTACATCA CCACAGACCC TGAACAAAAC 
GAGCCAAACG TAAACACTGA TAAATCTCTG CCCATCAGAC GTCATGTGGA ACCAGATGGA 
GGAGCAAATT CGCTGAACGT CTCCTCTCCG TCGGGTCAAA ACTTCTCCAT GAACAGCTCG 
ACTGCAGCCC GTCCTGCTCT GGATGTCGAC TCTCTGTTTG GTGACAGCAG CATCCAGCGA 
ATCATCGACC GATACACGAG AGAGCTCAAC TTCTCCCTCA GCACCACCGG GAGAGCCGCA 
GACAGTGAAG GCTCTCATGT GGACGATCCC GGCTCAGTCT CTCAGCAGTC TTTGGTTCGA 
GTCTCAGAGA GGAGTCGGTG TCTTCCCTCA GACGCAGCAG CAGCACAGAG GAGTGTCCAG 
GAATGGGACA ACACAGTCGA TCCAATCCTG GAGCACTTCT CAGTTGAGGG CACAGAGCAG 
GACTCTTTCA GACCTCTGAT TGGCCGGCTG GCAGACCAAT CATCCTGCTT GGCTGTCGAC 
CACAGGGACT CGACCATGGA GCAGCTGGTC GGTCAGCCAT CGGCTCACTC GTCCATGATC 
GGTCAGCTGC CCGGGCTGCC GGTGGGCCTC GATCAAGGCG GATGGGATTC CACTCTGAGC 
CGGATGATTG GTCGACTCTC CCATCAGTCC AGCCCTCATT GGCTGAGTGG AGGCCACGAC 
TTCTATGCGG GTCAGATGGT GTCAGAGCGA TCAGCCACGT GGTTGGATGA AGAGAGCCGG 
ATGAGACCGC TGGTCGGAGA GCTGGATGAG TCCACTGGAC ACCACAGTGG GAGCTCAGGT 
GAAAGAACTC GCGTGGATCC CGATGTTTCC ACTGAGGCCA GTGTGCCGTC ATTCCCAGCG 
TTGCCTCCTG AAGCGTCGTC ACACAGCGCC TCTGACCCGG CTGTGAATCC ACACCCCCAG 
GACCAGAACC AAACCAGTCC AATGGACCTG GGCCCAGAGC GGACTGAAGA CTCGTTCCAC 
CTGCTCGTGG CTGAAGTCAC CCACAACGAA ACAGCCGAGC CCTCCGTCAA CTTCCACGCT 
GTGCCTGACT CTCCTGAAGG TCGGCCAGCC AGCTGTGAAC AGAGCGCCTC TGCACCGCCT 
GAAGAGTTTG AAACCCACAA CGACCCGTCC ATCGAGTCTG AACTGTCTCC TGAGCGCCTC 
AGGACAGACA AGCCGCCCAG TTGCCTGACG CCGCCCCACG CCTTACATGA GTCTTTCTCC 
CAGCTCATCA CCTCCCAGCA CCACCCCCAT GAGGTGGAGC TAACCGCTCT GAGTCTGTCA 
AATCTCACCA TGTGTGATGA TGCGCTGACT CTGAAGCTGC AGCCTGAGGA ACCGTTCAGA 
GAGCTGGAGT CTGAGCAGAC TGTTCGTCCT GCCTCAGAGA AGCTGTCGGA GGCTGGCGGT 
GAGAAAGGCA TCCTGGAGCA GTCGGAGATA ACACTCGTGA GCCTGACAGA CACAACTCTG 
CAGGACCAAG AGACGACCAT CACCGACGAA GACGGAGTCT GGGACGGCAA ACATCCAGAC 
AGGGTGATGG AGGAAGGACA GAAAGAGACA GAGGAGTCGC ACTCAGTGGA ACAGACTCCA 
GAGGACGAGA ACCAAACACC TCCAGTGACC TTCCTGGAGT TTCAGTGGGG TCCGAGCCAA 
GACCTGCAGC AGGTCTACCA GCAGAAACGA CAAGCACTGC TCCAGAGATC ATCGCACAGG 
GTCGAGGAAA TCAAGTCCAG AAGAGATGGT CCAAAGGTTC AGCCTGAGGT CCAAGCTCCA 
CCCAGAGAGC AGTCTAAAGC TAAAGACTCT CAACCTGTCA GCTGTAAATC AAAGACTAAG 
ACTGAACCTC AGCCTAAGAC GAACACAAAG TCAGAGGGAG GACGGGCTGA GCAACAGACG 
TCTGACAAGT CTGGTTTCAA GCAGAAAAAG GTCCAGTGTC GACCTTCAGT GAGCGACTGC 
AGGCTGAAGA TCTGCACACC GGAGCAGAGG AAGCTGGATG TTACTGAGAT GCATCAGAGG 
ACTCAGAGAC TGTACGAGCA GCTGGAGGAG GTGAAGCATC AGAAAGCAGC GAGGAGCCGA 
CAGGAGGCGT CTGCAGAGAA CAGGCTGAAG GCCAAAGAGT TCCACAAGAA AACGTTACAG 
AAACTTCGTG CCAAGACGAC TCGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep295 ( XM_027279902.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027135703.1 Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X3, mRNA.

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CloneID	OLa221270
Clone ID Related Accession (Same CDS sequence)	XM_027279902.1
Accession Version	XM_027279902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	centrosomal protein of 295 kDa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040017.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA AAGTTTCCCA GCTCAGACTG AGCCCCAACG AGGAGGCCAG GCTGATCCGA 
GAGGAGCATG AGAGGAGGAG GAAGCTGCGA ATACAGCAGG TGCGGGAGCA GCAGCGGTAC 
ATCGCGCTGC AGATCCGCCG AGAGGTCGAG TTGAGGCGGC GGCGTGAGCT CGAGCAGCTG 
AAGGAGGAGC TGAGGGACGA CTGGGAGCGA CAGCAGAGGG AGAAACTCAA CACACTGCAG 
AAACTTTACC AGGAGAGCCT CCAGCTTCTC GGCCAAGGAC ACAGGAGCGC AAAAGAAAAC 
GAACCCGACC TGGCAGCCAT AGCTCAGAGG GAGGAGGAAA ATCACAGCAA AGCAGAGGAG 
CGTTATCGAG AAGCTCTGAA TGAGCTCAAA TCACAGAGGC TTAAAGACCA AGAGAGGCAG 
AGCCGTTCTA TCAATGCCAG GAAGAAGGCG CTACAGGCAG AAAAGGAGAG AGCGGCAAAA 
GTGGCCAGCC TCCCGCCGCC GCCGCCAAAC CCCATTCAGA ACATCGATTC AAAGAAGCCT 
CATGTGGTAA AGAAGTCTGA TGTGAGCGCC TTTGCTGCTA CACATTACCA CATGCCTGAG 
AGCACAGTGG ACAGAGAGGC AGACCCAACA CAGCCTAACG CTCACGAGGA AGCTGAGCTG 
GAGGCGAGGA GAATGCAGGA CCTCCAGAGG GAGGAGAAGC AGAGGAGAGA GGAGCAGCTG 
GAGAAGGCTC GTCTCAGAGG GAAGCAGGCT CTGAGGAGGG AACACCTCGT GCAGGACCGT 
GAGCGCTTGC TCTTGGAGCT GGAACACATG CAGCAGACAG ACCTGCTGAG GAGGAGACAG 
CAGGTGTCAC ACATGCCTGC TCAGATCTTC CAGCCTCTCT ACAAGAGACA GGAGATGAGG 
GAGGACTTCC AGAGGGAGAT GGAGTTCGCC TTTGAGGACA TGTACACTGG AGAGAGGAGG 
GTTAAAGGTG ATCTGGTGGT CCAGCTGGTA CCGGAGCCTC TGCCAGCTCT GTCCACAGGC 
AGCCAAGACC AGGACCAGGA GCTGGACATC ACGCTGGACG AAATCCCCAC ACCAGCAGCA 
GAAAACACAC ACCATGACAC TGAGCAAGAA GCTGGAAGCC CTGAGCAGGA AGCATCTGCA 
CGAGCAGAGA CCTCCAGACC TGCTCCCAGA CGGGCGTTGA AGAAACTCCT GGATCGTATC 
AGGAGCCAGC GGACTCACTG GAGCGAACAC AACGGTCACG TCCCCGCAGC CGACTCGCCG 
ACCGCCTTCA CCAATCAGAT ACCAGAGCAC GACACAACCA TCGACACGGG ATCTCTGACC 
AGTGAGGAGA AGGACAAACT CCCTGAACCC GCCCGCTCTC CACCCGTTTC AGAGACAACA 
GAGCCGTCAG CTGAAGCAGA AACTTCGGCT CCTGTCCGAC TCCAAGAATT TACAGACGAA 
CGAAGGAAAA GGGAGTTGGA GCTCGAGCAG GAGAAGCAGC AGCAGGTGGT GTTGATCCAG 
GAGTTAGAGG AGCAGAAGGC CAAACTGGAG CAGATGCTGT TTGAAGCACA GCAGGAGAGA 
GAGCACCTAA AGGCTGCTGT GACCCAGGAA GTGCCTGTCC TCGACCAGGA AGTCACCTCC 
GTCTCCTCCG GGCTGTCCAG TGAGCTGGTG CCTCCTCCGG GTGAAGATGA ACACAGCAGA 
AGGATTCGAG AATACCAACA GAGGCTGCTG GAACAGAACA GAATTCATCA GAGGTCGGTG 
GAGGTGGCTC GTCAGCGGCT GGAGGAATAC CAGCGAGCTC TACAGATCCG TTACAGCATG 
ACCACCAAAC AGTCGCTGCC CTCCGTCGTG CCTCCAGGAC TCGTTCACCC GCCGCTACAC 
AGTACTGGGC CTGTTCATCT TCCTGCTCCT CCGTCACTCC CAACAACTCC TGCAGTGCCT 
GCATACGTCC CTGCCAGACC ACACCCCTCT GTGGAAGTCA CTACAAGAGA GGCTGACATG 
GTGGCTTCAC CTCCCCACCT TCCTGGCTCC AGTTTGAGGG TTTGCTCCAG GTTGCTGCCA 
GATGAAGTGG AATCTGTTTG GAACAGAGCG AAGAACCAGA AACCCGATGT TTCTACCTGG 
CTGACTGACA ACATAATGGA GAGAGTGACT GAGCACCTTC CAGACAGCGT GAAACCCGTC 
TCAGTCACCA TGAAGCCACC GCCCCACAAA CTGTCCACAA CACGTGAGTC TACCAGCATC 
CTGCTTCAGC CGACCTCTCA TCCCATCCAA GTCATCGGCC CCAGCATTGC AGATAGAACA 
CCTCTGTTTC CAGGCCATGT AGCGGTTAAG CCGCATGTCT CCCTGCTGAC TGGGACCCTG 
AGCTCCAGAG AGGACGACAT GGAGAGGCAG AGACGGGAGC TGCAGAGGCA ACAAGAGGAG 
GAGGAGGAGG AGGAGAGGCA GAGATGGGAG CTGCAGGAGG TTCAGAGACG ATTGCTGGAG 
CAGAGGGAGG CAGTGGAAGT GCAACAGAGG CAGAAAGAAG AGGAGAGGCA GAGACAGGAG 
CTGCAGAGAC GAATGCAGGA GCAAAGAGCG GCAGTAGAGC TCCAGCAGAA GCAGCAAGAA 
GAGGAGAGGC AGAGACGGTT GCTGGAGCAG AGGGAGGCGA TGGAGGTGCA ACAAAGGCAG 
ACAGAGGAGG AGAAGCAGAG GCGGGAGCTG CAGGAGGTTC AGAGACGGTT GTTGGAGCAG 
AGGGAGGCAG TGGAGCTGCA GAAGAAGCAA CAAGAAGAGG AGAGGCAGAG GCGGGAAGTG 
GAGATGGAGG AGATGAGGCG ACAGAAGGAG ACGTTACAGG CTCTGATTCA TACTGACGCA 
CAGCCAGGTG CAGAGGTCTC CAGTGAAGTG CCGCTTTCAG AGAGCATCGG TCCAACTCGA 
CGTCAGTTAC TTGCATCACT GCTGCGAGCC ATACAGGAAA CTAACGGAGG AACTCTATCA 
CATCTAGAAG AGCCGCAGGA GGAAGATGAC TCTCCTCAAC AACCATCATC ATCTTACAGC 
GTCGAGACTG ACCCCGTCTT TCAGAGCAGT GTTCCTGCTG GACCTCTCCC AGCATCAGGC 
CTCCTGCCTC CTCCTCGAGC AGCGAAGCCT CCAGTGACTC GCGTCAGGCT GGGCGTCATG 
AAGATGATGA CTGAACAACA TGAGCTCAGT GCTATTCAAG AGGTGGAGAC GCCAGTCAAC 
AGCAGCCTGG TCACAGGTCT GGAGGACAGT GTGAAGCTTC CTTCACAGAC TGTGGGCTGG 
GATCCACAGG AGGAGTCCGA CTCGTCGGTG TCCTCTGACA GGACTCTGCA GACGCCCTCT 
TGGTCCAGCA GCGAGCAGCG GCCTGCTGAA AGACGAGAGG CGAGCTCGAG GAGGTACAGC 
CATCTGGATC TGCTGATGGA GGCTGGAACA TCTCCAGAAT CCTCAGAGTC ATCTGATTCA 
GTCGTGAGGA AAACGTCACG GCTTTCCTCT GACTCTGGCA GAGGAGCTGA CTACTCCGGA 
CCTGCGATCA CGAGCTACAG ATCCTTCACA GAGTCTGCAC AGAGGCCTCC TGACTCTGAC 
TGCCTCTCCT CCACCACCAT CTCCAGCGGC AGCTACATCA CCACAGACCC TGAACAAAAC 
GAGCCAAACG TAAACACTGA TAAATCTCTG CCCATCAGAC GTCATGTGGA ACCAGATGGA 
GGAGCAAATT CGCTGAACGT CTCCTCTCCG TCGGGTCAAA ACTTCTCCAT GAACAGCTCG 
ACTGCAGCCC GTCCTGCTCT GGATGTCGAC TCTCTGTTTG GTGACAGCAG CATCCAGCGA 
ATCATCGACC GATACACGAG AGAGCTCAAC TTCTCCCTCA GCACCACCGG GAGAGCCGCA 
GACAGTGAAG GCTCTCATGT GGACGATCCC GGCTCAGTCT CTCAGCAGTC TTTGGTTCGA 
GTCTCAGAGA GGAGTCGGTG TCTTCCCTCA GACGCAGCAG CAGCACAGAG GAGTGTCCAG 
GAATGGGACA ACACAGTCGA TCCAATCCTG GAGCACTTCT CAGTTGAGGG CACAGAGCAG 
GACTCTTTCA GACCTCTGAT TGGCCGGCTG GCAGACCAAT CATCCTGCTT GGCTGTCGAC 
CACAGGGACT CGACCATGGA GCAGCTGGTC GGTCAGCCAT CGGCTCACTC GTCCATGATC 
GGTCAGCTGC CCGGGCTGCC GGTGGGCCTC GATCAAGGCG GATGGGATTC CACTCTGAGC 
CGGATGATTG GTCGACTCTC CCATCAGTCC AGCCCTCATT GGCTGAGTGG AGGCCACGAC 
TTCTATGCGG GTCAGATGGT GTCAGAGCGA TCAGCCACGT GGTTGGATGA AGAGAGCCGG 
ATGAGACCGC TGGTCGGAGA GCTGGATGAG TCCACTGGAC ACCACAGTGG GAGCTCAGGT 
GAAAGAACTC GCGTGGATCC CGATGTTTCC ACTGAGGCCA GTGTGCCGTC ATTCCCAGCG 
TTGCCTCCTG AAGCGTCGTC ACACAGCGCC TCTGACCCGG CTGTGAATCC ACACCCCCAG 
GACCAGAACC AAACCAGTCC AATGGACCTG GGCCCAGAGC GGACTGAAGA CTCGTTCCAC 
CTGCTCGTGG CTGAAGTCAC CCACAACGAA ACAGCCGAGC CCTCCGTCAA CTTCCACGCT 
GTGCCTGACT CTCCTGAAGG TCGGCCAGCC AGCTGTGAAC AGAGCGCCTC TGCACCGCCT 
GAAGAGTTTG AAACCCACAA CGACCCGTCC ATCGAGTCTG AACTGTCTCC TGAGCGCCTC 
AGGACAGACA AGCCGCCCAG TTGCCTGACG CCGCCCCACG CCTTACATGA GTCTTTCTCC 
CAGCTCATCA CCTCCCAGCA CCACCCCCAT GAGGTGGAGC TAACCGCTCT GAGTCTGTCA 
AATCTCACCA TGTGTGATGA TGCGCTGACT CTGAAGCTGC AGCCTGAGGA ACCGTTCAGA 
GAGCTGGAGT CTGAGCAGAC TGTTCGTCCT GCCTCAGAGA AGCTGTCGGA GGCTGGCGGT 
GAGAAAGGCA TCCTGGAGCA GTCGGAGATA ACACTCGTGA GCCTGACAGA CACAACTCTG 
CAGGACCAAG AGACGACCAT CACCGACGAA GACGGAGTCT GGGACGGCAA ACATCCAGAC 
AGGGTGATGG AGGAAGGACA GAAAGAGACA GAGGAGTCGC ACTCAGTGGA ACAGACTCCA 
GAGGACGAGA ACCAAACACC TCCAGTGACC TTCCTGGAGT TTCAGTGGGG TCCGAGCCAA 
GACCTGCAGC AGGTCTACCA GCAGAAACGA CAAGCACTGC TCCAGAGATC ATCGCACAGG 
GTCGAGGAAA TCAAGTCCAG AAGAGATGGT CCAAAGGTTC AGCCTGAGGT CCAAGCTCCA 
CCCAGAGAGC AGTCTAAAGC TAAAGACTCT CAACCTGTCA GCTGTAAATC AAAGACTAAG 
ACTGAACCTC AGCCTAAGAC GAACACAAAG TCAGAGGGAG GACGGGCTGA GCAACAGACG 
TCTGACAAGT CTGGTTTCAA GCAGAAAAAG GTCCAGTGTC GACCTTCAGT GAGCGACTGC 
AGGCTGAAGA TCTGCACACC GGAGCAGAGG AAGCTGGATG TTACTGAGAT GCATCAGAGG 
ACTCAGAGAC TGTACGAGCA GCTGGAGGAG GTGAAGCATC AGAAAGCAGC GAGGAGCCGA 
CAGGAGGCGT CTGCAGAGAA CAGGCTGAAG GCCAAAGAGT TCCACAAGAA AACGTTACAG 
AAACTTCGTG CCAAGACGAC TCGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027135703.1
CDS	283..6012
Translation	MKRKVSQLRLSPNEEARLIREEHERRRKLRIQQVREQQRYIALQIRREVELRRRRELEQLKEELRDDWERQQREKLNTLQKLYQESLQLLGQGHRSAKENEPDLAAIAQREEENHSKAEERYREALNELKSQRLKDQERQSRSINARKKALQAEKERAAKVASLPPPPPNPIQNIDSKKPHVVKKSDVSAFAATHYHMPESTVDREADPTQPNAHEEAELEARRMQDLQREEKQRREEQLEKARLRGKQALRREHLVQDRERLLLELEHMQQTDLLRRRQQVSHMPAQIFQPLYKRQEMREDFQREMEFAFEDMYTGERRVKGDLVVQLVPEPLPALSTGSQDQDQELDITLDEIPTPAAENTHHDTEQEAGSPEQEASARAETSRPAPRRALKKLLDRIRSQRTHWSEHNGHVPAADSPTAFTNQIPEHDTTIDTGSLTSEEKDKLPEPARSPPVSETTEPSAEAETSAPVRLQEFTDERRKRELELEQEKQQQVVLIQELEEQKAKLEQMLFEAQQEREHLKAAVTQEVPVLDQEVTSVSSGLSSELVPPPGEDEHSRRIREYQQRLLEQNRIHQRSVEVARQRLEEYQRALQIRYSMTTKQSLPSVVPPGLVHPPLHSTGPVHLPAPPSLPTTPAVPAYVPARPHPSVEVTTREADMVASPPHLPGSSLRVCSRLLPDEVESVWNRAKNQKPDVSTWLTDNIMERVTEHLPDSVKPVSVTMKPPPHKLSTTRESTSILLQPTSHPIQVIGPSIADRTPLFPGHVAVKPHVSLLTGTLSSREDDMERQRRELQRQQEEEEEEERQRWELQEVQRRLLEQREAVEVQQRQKEEERQRQELQRRMQEQRAAVELQQKQQEEERQRRLLEQREAMEVQQRQTEEEKQRRELQEVQRRLLEQREAVELQKKQQEEERQRREVEMEEMRRQKETLQALIHTDAQPGAEVSSEVPLSESIGPTRRQLLASLLRAIQETNGGTLSHLEEPQEEDDSPQQPSSSYSVETDPVFQSSVPAGPLPASGLLPPPRAAKPPVTRVRLGVMKMMTEQHELSAIQEVETPVNSSLVTGLEDSVKLPSQTVGWDPQEESDSSVSSDRTLQTPSWSSSEQRPAERREASSRRYSHLDLLMEAGTSPESSESSDSVVRKTSRLSSDSGRGADYSGPAITSYRSFTESAQRPPDSDCLSSTTISSGSYITTDPEQNEPNVNTDKSLPIRRHVEPDGGANSLNVSSPSGQNFSMNSSTAARPALDVDSLFGDSSIQRIIDRYTRELNFSLSTTGRAADSEGSHVDDPGSVSQQSLVRVSERSRCLPSDAAAAQRSVQEWDNTVDPILEHFSVEGTEQDSFRPLIGRLADQSSCLAVDHRDSTMEQLVGQPSAHSSMIGQLPGLPVGLDQGGWDSTLSRMIGRLSHQSSPHWLSGGHDFYAGQMVSERSATWLDEESRMRPLVGELDESTGHHSGSSGERTRVDPDVSTEASVPSFPALPPEASSHSASDPAVNPHPQDQNQTSPMDLGPERTEDSFHLLVAEVTHNETAEPSVNFHAVPDSPEGRPASCEQSASAPPEEFETHNDPSIESELSPERLRTDKPPSCLTPPHALHESFSQLITSQHHPHEVELTALSLSNLTMCDDALTLKLQPEEPFRELESEQTVRPASEKLSEAGGEKGILEQSEITLVSLTDTTLQDQETTITDEDGVWDGKHPDRVMEEGQKETEESHSVEQTPEDENQTPPVTFLEFQWGPSQDLQQVYQQKRQALLQRSSHRVEEIKSRRDGPKVQPEVQAPPREQSKAKDSQPVSCKSKTKTEPQPKTNTKSEGGRAEQQTSDKSGFKQKKVQCRPSVSDCRLKICTPEQRKLDVTEMHQRTQRLYEQLEEVKHQKAARSRQEASAENRLKAKEFHKKTLQKLRAKTTRQ

Target ORF information:

RefSeq Version	XM_027279902.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027279902.1

ORF Insert Sequence:

ATGAAGAGGA AAGTTTCCCA GCTCAGACTG AGCCCCAACG AGGAGGCCAG GCTGATCCGA 
GAGGAGCATG AGAGGAGGAG GAAGCTGCGA ATACAGCAGG TGCGGGAGCA GCAGCGGTAC 
ATCGCGCTGC AGATCCGCCG AGAGGTCGAG TTGAGGCGGC GGCGTGAGCT CGAGCAGCTG 
AAGGAGGAGC TGAGGGACGA CTGGGAGCGA CAGCAGAGGG AGAAACTCAA CACACTGCAG 
AAACTTTACC AGGAGAGCCT CCAGCTTCTC GGCCAAGGAC ACAGGAGCGC AAAAGAAAAC 
GAACCCGACC TGGCAGCCAT AGCTCAGAGG GAGGAGGAAA ATCACAGCAA AGCAGAGGAG 
CGTTATCGAG AAGCTCTGAA TGAGCTCAAA TCACAGAGGC TTAAAGACCA AGAGAGGCAG 
AGCCGTTCTA TCAATGCCAG GAAGAAGGCG CTACAGGCAG AAAAGGAGAG AGCGGCAAAA 
GTGGCCAGCC TCCCGCCGCC GCCGCCAAAC CCCATTCAGA ACATCGATTC AAAGAAGCCT 
CATGTGGTAA AGAAGTCTGA TGTGAGCGCC TTTGCTGCTA CACATTACCA CATGCCTGAG 
AGCACAGTGG ACAGAGAGGC AGACCCAACA CAGCCTAACG CTCACGAGGA AGCTGAGCTG 
GAGGCGAGGA GAATGCAGGA CCTCCAGAGG GAGGAGAAGC AGAGGAGAGA GGAGCAGCTG 
GAGAAGGCTC GTCTCAGAGG GAAGCAGGCT CTGAGGAGGG AACACCTCGT GCAGGACCGT 
GAGCGCTTGC TCTTGGAGCT GGAACACATG CAGCAGACAG ACCTGCTGAG GAGGAGACAG 
CAGGTGTCAC ACATGCCTGC TCAGATCTTC CAGCCTCTCT ACAAGAGACA GGAGATGAGG 
GAGGACTTCC AGAGGGAGAT GGAGTTCGCC TTTGAGGACA TGTACACTGG AGAGAGGAGG 
GTTAAAGGTG ATCTGGTGGT CCAGCTGGTA CCGGAGCCTC TGCCAGCTCT GTCCACAGGC 
AGCCAAGACC AGGACCAGGA GCTGGACATC ACGCTGGACG AAATCCCCAC ACCAGCAGCA 
GAAAACACAC ACCATGACAC TGAGCAAGAA GCTGGAAGCC CTGAGCAGGA AGCATCTGCA 
CGAGCAGAGA CCTCCAGACC TGCTCCCAGA CGGGCGTTGA AGAAACTCCT GGATCGTATC 
AGGAGCCAGC GGACTCACTG GAGCGAACAC AACGGTCACG TCCCCGCAGC CGACTCGCCG 
ACCGCCTTCA CCAATCAGAT ACCAGAGCAC GACACAACCA TCGACACGGG ATCTCTGACC 
AGTGAGGAGA AGGACAAACT CCCTGAACCC GCCCGCTCTC CACCCGTTTC AGAGACAACA 
GAGCCGTCAG CTGAAGCAGA AACTTCGGCT CCTGTCCGAC TCCAAGAATT TACAGACGAA 
CGAAGGAAAA GGGAGTTGGA GCTCGAGCAG GAGAAGCAGC AGCAGGTGGT GTTGATCCAG 
GAGTTAGAGG AGCAGAAGGC CAAACTGGAG CAGATGCTGT TTGAAGCACA GCAGGAGAGA 
GAGCACCTAA AGGCTGCTGT GACCCAGGAA GTGCCTGTCC TCGACCAGGA AGTCACCTCC 
GTCTCCTCCG GGCTGTCCAG TGAGCTGGTG CCTCCTCCGG GTGAAGATGA ACACAGCAGA 
AGGATTCGAG AATACCAACA GAGGCTGCTG GAACAGAACA GAATTCATCA GAGGTCGGTG 
GAGGTGGCTC GTCAGCGGCT GGAGGAATAC CAGCGAGCTC TACAGATCCG TTACAGCATG 
ACCACCAAAC AGTCGCTGCC CTCCGTCGTG CCTCCAGGAC TCGTTCACCC GCCGCTACAC 
AGTACTGGGC CTGTTCATCT TCCTGCTCCT CCGTCACTCC CAACAACTCC TGCAGTGCCT 
GCATACGTCC CTGCCAGACC ACACCCCTCT GTGGAAGTCA CTACAAGAGA GGCTGACATG 
GTGGCTTCAC CTCCCCACCT TCCTGGCTCC AGTTTGAGGG TTTGCTCCAG GTTGCTGCCA 
GATGAAGTGG AATCTGTTTG GAACAGAGCG AAGAACCAGA AACCCGATGT TTCTACCTGG 
CTGACTGACA ACATAATGGA GAGAGTGACT GAGCACCTTC CAGACAGCGT GAAACCCGTC 
TCAGTCACCA TGAAGCCACC GCCCCACAAA CTGTCCACAA CACGTGAGTC TACCAGCATC 
CTGCTTCAGC CGACCTCTCA TCCCATCCAA GTCATCGGCC CCAGCATTGC AGATAGAACA 
CCTCTGTTTC CAGGCCATGT AGCGGTTAAG CCGCATGTCT CCCTGCTGAC TGGGACCCTG 
AGCTCCAGAG AGGACGACAT GGAGAGGCAG AGACGGGAGC TGCAGAGGCA ACAAGAGGAG 
GAGGAGGAGG AGGAGAGGCA GAGATGGGAG CTGCAGGAGG TTCAGAGACG ATTGCTGGAG 
CAGAGGGAGG CAGTGGAAGT GCAACAGAGG CAGAAAGAAG AGGAGAGGCA GAGACAGGAG 
CTGCAGAGAC GAATGCAGGA GCAAAGAGCG GCAGTAGAGC TCCAGCAGAA GCAGCAAGAA 
GAGGAGAGGC AGAGACGGTT GCTGGAGCAG AGGGAGGCGA TGGAGGTGCA ACAAAGGCAG 
ACAGAGGAGG AGAAGCAGAG GCGGGAGCTG CAGGAGGTTC AGAGACGGTT GTTGGAGCAG 
AGGGAGGCAG TGGAGCTGCA GAAGAAGCAA CAAGAAGAGG AGAGGCAGAG GCGGGAAGTG 
GAGATGGAGG AGATGAGGCG ACAGAAGGAG ACGTTACAGG CTCTGATTCA TACTGACGCA 
CAGCCAGGTG CAGAGGTCTC CAGTGAAGTG CCGCTTTCAG AGAGCATCGG TCCAACTCGA 
CGTCAGTTAC TTGCATCACT GCTGCGAGCC ATACAGGAAA CTAACGGAGG AACTCTATCA 
CATCTAGAAG AGCCGCAGGA GGAAGATGAC TCTCCTCAAC AACCATCATC ATCTTACAGC 
GTCGAGACTG ACCCCGTCTT TCAGAGCAGT GTTCCTGCTG GACCTCTCCC AGCATCAGGC 
CTCCTGCCTC CTCCTCGAGC AGCGAAGCCT CCAGTGACTC GCGTCAGGCT GGGCGTCATG 
AAGATGATGA CTGAACAACA TGAGCTCAGT GCTATTCAAG AGGTGGAGAC GCCAGTCAAC 
AGCAGCCTGG TCACAGGTCT GGAGGACAGT GTGAAGCTTC CTTCACAGAC TGTGGGCTGG 
GATCCACAGG AGGAGTCCGA CTCGTCGGTG TCCTCTGACA GGACTCTGCA GACGCCCTCT 
TGGTCCAGCA GCGAGCAGCG GCCTGCTGAA AGACGAGAGG CGAGCTCGAG GAGGTACAGC 
CATCTGGATC TGCTGATGGA GGCTGGAACA TCTCCAGAAT CCTCAGAGTC ATCTGATTCA 
GTCGTGAGGA AAACGTCACG GCTTTCCTCT GACTCTGGCA GAGGAGCTGA CTACTCCGGA 
CCTGCGATCA CGAGCTACAG ATCCTTCACA GAGTCTGCAC AGAGGCCTCC TGACTCTGAC 
TGCCTCTCCT CCACCACCAT CTCCAGCGGC AGCTACATCA CCACAGACCC TGAACAAAAC 
GAGCCAAACG TAAACACTGA TAAATCTCTG CCCATCAGAC GTCATGTGGA ACCAGATGGA 
GGAGCAAATT CGCTGAACGT CTCCTCTCCG TCGGGTCAAA ACTTCTCCAT GAACAGCTCG 
ACTGCAGCCC GTCCTGCTCT GGATGTCGAC TCTCTGTTTG GTGACAGCAG CATCCAGCGA 
ATCATCGACC GATACACGAG AGAGCTCAAC TTCTCCCTCA GCACCACCGG GAGAGCCGCA 
GACAGTGAAG GCTCTCATGT GGACGATCCC GGCTCAGTCT CTCAGCAGTC TTTGGTTCGA 
GTCTCAGAGA GGAGTCGGTG TCTTCCCTCA GACGCAGCAG CAGCACAGAG GAGTGTCCAG 
GAATGGGACA ACACAGTCGA TCCAATCCTG GAGCACTTCT CAGTTGAGGG CACAGAGCAG 
GACTCTTTCA GACCTCTGAT TGGCCGGCTG GCAGACCAAT CATCCTGCTT GGCTGTCGAC 
CACAGGGACT CGACCATGGA GCAGCTGGTC GGTCAGCCAT CGGCTCACTC GTCCATGATC 
GGTCAGCTGC CCGGGCTGCC GGTGGGCCTC GATCAAGGCG GATGGGATTC CACTCTGAGC 
CGGATGATTG GTCGACTCTC CCATCAGTCC AGCCCTCATT GGCTGAGTGG AGGCCACGAC 
TTCTATGCGG GTCAGATGGT GTCAGAGCGA TCAGCCACGT GGTTGGATGA AGAGAGCCGG 
ATGAGACCGC TGGTCGGAGA GCTGGATGAG TCCACTGGAC ACCACAGTGG GAGCTCAGGT 
GAAAGAACTC GCGTGGATCC CGATGTTTCC ACTGAGGCCA GTGTGCCGTC ATTCCCAGCG 
TTGCCTCCTG AAGCGTCGTC ACACAGCGCC TCTGACCCGG CTGTGAATCC ACACCCCCAG 
GACCAGAACC AAACCAGTCC AATGGACCTG GGCCCAGAGC GGACTGAAGA CTCGTTCCAC 
CTGCTCGTGG CTGAAGTCAC CCACAACGAA ACAGCCGAGC CCTCCGTCAA CTTCCACGCT 
GTGCCTGACT CTCCTGAAGG TCGGCCAGCC AGCTGTGAAC AGAGCGCCTC TGCACCGCCT 
GAAGAGTTTG AAACCCACAA CGACCCGTCC ATCGAGTCTG AACTGTCTCC TGAGCGCCTC 
AGGACAGACA AGCCGCCCAG TTGCCTGACG CCGCCCCACG CCTTACATGA GTCTTTCTCC 
CAGCTCATCA CCTCCCAGCA CCACCCCCAT GAGGTGGAGC TAACCGCTCT GAGTCTGTCA 
AATCTCACCA TGTGTGATGA TGCGCTGACT CTGAAGCTGC AGCCTGAGGA ACCGTTCAGA 
GAGCTGGAGT CTGAGCAGAC TGTTCGTCCT GCCTCAGAGA AGCTGTCGGA GGCTGGCGGT 
GAGAAAGGCA TCCTGGAGCA GTCGGAGATA ACACTCGTGA GCCTGACAGA CACAACTCTG 
CAGGACCAAG AGACGACCAT CACCGACGAA GACGGAGTCT GGGACGGCAA ACATCCAGAC 
AGGGTGATGG AGGAAGGACA GAAAGAGACA GAGGAGTCGC ACTCAGTGGA ACAGACTCCA 
GAGGACGAGA ACCAAACACC TCCAGTGACC TTCCTGGAGT TTCAGTGGGG TCCGAGCCAA 
GACCTGCAGC AGGTCTACCA GCAGAAACGA CAAGCACTGC TCCAGAGATC ATCGCACAGG 
GTCGAGGAAA TCAAGTCCAG AAGAGATGGT CCAAAGGTTC AGCCTGAGGT CCAAGCTCCA 
CCCAGAGAGC AGTCTAAAGC TAAAGACTCT CAACCTGTCA GCTGTAAATC AAAGACTAAG 
ACTGAACCTC AGCCTAAGAC GAACACAAAG TCAGAGGGAG GACGGGCTGA GCAACAGACG 
TCTGACAAGT CTGGTTTCAA GCAGAAAAAG GTCCAGTGTC GACCTTCAGT GAGCGACTGC 
AGGCTGAAGA TCTGCACACC GGAGCAGAGG AAGCTGGATG TTACTGAGAT GCATCAGAGG 
ACTCAGAGAC TGTACGAGCA GCTGGAGGAG GTGAAGCATC AGAAAGCAGC GAGGAGCCGA 
CAGGAGGCGT CTGCAGAGAA CAGGCTGAAG GCCAAAGAGT TCCACAAGAA AACGTTACAG 
AAACTTCGTG CCAAGACGAC TCGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep295 ( XM_010755163.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010753465.2 Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLa117478
Clone ID Related Accession (Same CDS sequence)	XM_010755163.2
Accession Version	XM_010755163.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	centrosomal protein of 295 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609891.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010755163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA AAGTTTCCCA GCTCAGACTG AGCCCCAACG AGGAGGCCAG GCTGATCCGA 
GAGGAGCATG AGAGGAGGAG GAAGCTGCGA ATACAGCAGG TGCGGGAGCA GCAGCGGTAC 
ATCGCGCTGC AGATCCGCCG AGAGGTCGAG TTGAGGCGGC GGCGTGAGCT CGAGCAGCTG 
AAGGAGGAGC TGAGGGACGA CTGGGAGCGA CAGCAGAGGG AGAAACTCAA CACACTGCAG 
AAACTTTACC AGGAGAGCCT CCAGCTTCTC GGCCAAGGAC ACAGGAGCGC AAAAGAAAAC 
GAACCCGACC TGGCAGCCAT AGCTCAGAGG GAGGAGGAAA ATCACAGCAA AGCAGAGGAG 
CGTTATCGAG AAGCTCTGAA TGAGCTCAAA TCACAGAGGC TTAAAGACCG TGAGAGGCAG 
AGCCGTTCCA TCAATGCCAG GAAGAAGGCG CTACAGGCAG AAAAGGAGAG AGCGGCAAAA 
GTGGCCAGCC TCCCGCCGCC GCCGCCAAAC CCCATTCAGA ACATCGATTC AAAGAAGCCT 
CATGTGGTAA AGAAGTCTGA TGTGAGCGCC TTTGCTGCTA CACATTACCA CATGCCTGAG 
AGCACAGTGG ACAGAGAGGC AGACCCAACA CAGCCTAACG CTCACGAGGA AGCTGAGCTG 
GAGGCGAGGA GACTGCAGGA CCTCCAGAGG GAGGAGAAGC AGAGGAGAGA GGAGCAGCTG 
GAGAAGGCTC GTCTCAGAGG GAAGCAGGCT CTGAGGAGGG AACACCTCGT GCAGGACCGT 
GAGCGCTTGC TCTTGGAGCT GGAACACATG CAGCAGACAG ACCTGCTGAG GAGGAGACAG 
CAGGTGTCAC ACATGCCTGC TCAGATCTTC CAGCCTCTCT ACAAGAGACA GGAGATGAGG 
GAGGACTTCC AGAGGGAGAT GGAGTTCGCC TTTGAGGACA TGTACACTGG AGAGAGGAGG 
GTTAAAGGTG ATCTGGTGGT CCAGCTGGTA CCGGAGCCTC TGCCAGCTCT GTCCACAGGC 
AGCCAAGACC AGGACCAGGA GCTGGACATC ACGCTGGACG AAATCCCCAC ACCAGCAGCA 
GAAAACACAC ACCATGACAC TGAGCAAGAA GCTGGAAGCC CTGAGCAGGA AGCATCTGCA 
CGAGCAGAGA CCTCCAGACC TGCTCCCAGA CGGGCGTTGA AGAAACTCCT GGATCGTATC 
AGGAGCCAGC GGACTCACTG GAGCGAACAC AACGGTCACG TCCCCGCAGC CGACTCGCCG 
ACCGCCTTCA CCAATCAGAT ACCAGAGCAC GACACAACCA TCGACACGGG ATCTCTGACC 
AGTGAGGAGA AGGACAAACT CCCTGAACCC GCCCGCTCTC CACCCGCAGT TTCAGAGACA 
ACAGAGCCGT CAGCTGAAGC AGAAACTTCG GCTCCTGTCC GACTCCAAGA ATTTACAGAC 
GAACGAAGGA AAAGGGAGTT GGAGCTCGAG CAGGAGAAGC AGCAGCAGGT GGTGTTGATC 
CAGGAGTTAG AGGAGCAGAA GGCCAAACTG GAGCAGATGC TGCTCGAAGC ACAGCAGGAG 
AGAGAGCACC TGAAGGCTGC TGTGACCCAG GAAGTTCCTG TCCTCGACCA GGAAGTCACC 
TCCGTCTCCT CCGGGCTGTC CACTGAGCTG GTGCCTCCTC CGGGTGAAGA TGAACACAGC 
AGAAGGATTC GAGAATACCA ACAGAGGCTG CTGGAACAGA ACAGAATTCA CCAGAGGTCG 
GTGGAGGTGG CTCGTCAGCG GCTGGAGGAA TACCAGCGAG CTCTACAGAT TCGTTACAGC 
ATGACCAAAC AGTCGCTGCC CTCCGTCATG CCTCCAGGAC TCGTTCACCC GCCGCTACAC 
AGTACTTGGC CTGTTCATCT TCCTGCTCCT CCGTCACTCC CGACAACTCC TGCAGTGGTT 
GCATACGTCC CTGCCAGACC ACACCCCTCT GTGGAAGTCA CTACAAGAGA GGCTGACATG 
GTGGCTTCAC CTCCCCACCT TCCTGGCTCC AGTTTGAGGG TTTGCTCCAG GTTGCTGCCA 
GATGAAGTGG AATCTGTTTG GAACAGAGCG AAGAACCAGA AACCCGATGT TTCTACCTGG 
CTGACTGACA ACATAATGGA GAGAGTGACT GAGCACCTTC CAGACAGCGT GAAACCCGTC 
TCAGTCACCA TGAAGCCACC GCCCCACAAA CTGTCCACAA CACGTGAGTC TACCAGCATC 
CTGCTTCAGC CGACCTCTCA TCCCATCCAA GTCATCGGCC CCAGCATTGC AGATAGAACA 
CCTCTGTTTC CAGGCCATGT AGCGGTTAAG CCGCATGTCT CCCTGCTGAC TGGGCCCCTG 
AGCTCCAGAG AGGACGACAT GGAGAGGCAG AGGCAACAAG AGGAGGAGGA GGAGGAGGAG 
AGGCAGAGAT GGGAGCTGCA GGAGGTTCAG AGACGATTGC TGGAGCAGAG GGAGGCAGTG 
GAAGTGCAAC AGAGGCAGAA AGAAGAGGAG AGGCAGAGAC AGGAGCTGCA GAGACGAATG 
CAGGAGCAAA GAGCGGCAGT AGAGCTCCAG CAGAAGCAGC AAGAAGAGGA GAGGCAGAGA 
CGGTTGCTGG AGCAGAGGGA GGCGATGGAG GTGCAACAAA GGCAGACAGA GGAGGAGAAG 
CAGAGGCGGG AGCTGCAGGA GGTTCAGAGA CGGTTGTTGG AGCAGAGGGA GGCAGTGGAG 
CTGCAGAAGA AGCAACAAGA AGAGGAGAGG CAGAGGCGGG AAGTGGAGAT GGAGGAGATG 
AGGCGACAGA AGGAGACGTT ACAGGCTCTG ATTCATACTG ACGCACAGCC AGGTGCAGAG 
GTCTCCAGTG AAGTGCCGCT TTCAGAGAGC ATCGGTCCAA CTCGACGTCA GTTACTTGCA 
TCACTGCTGC GAGCCATACA GGAAACTAAC GGAGGAACTC TATCACATCT AGAAGAGCCG 
CAGGAGGAAG ATGACTCTCC TCAACAACCA TCATCATCTT ACAGCGTCGA GACTGACCCC 
GTCTTTCAGA GCAGTGTTCC TGCTGGACCT CTCCCAGCAT CAGGCCTCCT GCCTCCTCCT 
CGAGCAGCGA AGCCTCCAGT GACTCGCGTC AGGCTGGGCG TCATGAAGAT GATGACTGAA 
CAACATGAGC TCAGTGCTAT TCAAGAGGTG GAGACGCCAG TCAACAGCAG CCTGGTCACA 
GGTCTGGAGG ACAGTGTGAA GCTTCCTTCA CAGACTGTGG GCTGGGATCC ACAGGAGGAG 
TCCGACTCGT CGGTGTCCTC TGACAGGACT CTGCAGACGC CCTCTTGGTC CAGCAGCGAG 
CAGCGGCCTG CTGAAAGACG AGAGGCGAGC TCGAGGAGGT ACAGCCATCT GGATCTGCTG 
ATGGAGGCTG GAACATCTCC AGAATCCTCA GAGTCATCTG ATTCAGTCGT GAGGAAAACG 
TCACGGCTTT CCTCTGACTC TGGCAGAGGA GCTGACTACT CCGGACCTGC GATCACGAGC 
TACAGATCCT TCACAGAGTC TGCACAGAGG CCTCCTGACT CTGACTGCCT CTCCTCCACC 
ACCATCTCCA GCGGCAGCTA CATCACCACA GACCCTGAAC AAAACGAGCC AAACGTAAAC 
ACTGATAAAT CTCTGCCCAT CAGACGTCAT GTGGAACAAG ATGGAGGAGC AAATACGCTG 
AACGTCTCCT CTCAGTCGGT TCAAAACTTC TCCATGAACA GCTCGACTGC AGCCCGTCCT 
GCTCCGGATG TCGACTCTCT GTTTAGTGAC AGCAGCATCC AGCGAATCAT CGACCGATAC 
ACGAGAGAGC TCAACTTCTC CCTCAGCACC ACCGGGAGAG CCGCAGACAG TGAAGGCTCT 
CATGTGGACG ATCCCGGCTC AGTCTCTCAG CAGTCTTTGG TTCGAGTCTC AGAGAGGAGT 
CAGTGTCTTC CCTCAGACGC AGCAGCAGCA CAGAGGAGTG TCCAGGAATG GGACAACACA 
GTCGATCCAA TCCTGGAGCA CTTCTCAGTT GAGGGCACAG AGCAGGACTC CTTCAGACCT 
CTGATTGGCC GGCTGGCAGA CCAATCATCC TGCTTGGCTG TCGACCACAG GGACTCGACC 
ATGGAGCAGC TGGTCGGTCA ACCATCGGCT CACTCGTCCA TGATCGGTCA GCTGCCCGGG 
CTGCCGGTGG GCCTCGATCA AGGCGGATGG GATTCCACTC TGAGCCGGAT GATTGGTCGA 
CTCTCCCATC AGTCCAGCCC TCATTGGCTG AGTGGAGGCC ACGACTTCTA TGCGGGTCAG 
ATGGTGTCAG AGCGATCAGC CACGTGGTTG GATGAAGAGA GCCGGATGAG ACCGCTGGTC 
GGAGAGCTGG ATGAGTCCAC TGGACACCAC AGTGGGAGCT CAGGTGAAAG AACTCGCGTG 
GATCCCGATG TTTCCACTGA GGCCAGTGTG CCGTCATTTC CAGCGTTGCC TCCTGAAGCG 
TCGTCACACA GCGCCTCTGA CCCGGCTGTA AATCCACACC CACAGGACCA GAACCAAACC 
AGTCCAATGG ACCTGGGCCC AGAGCGGACT GAAGACTCGT TCCACCTGCT CGTGGCTGAA 
GTCACCCACA ACGAAACAGC CGAGCCCTCC GTCAACTTCC ACGCTGTGCC TGACTCTCCT 
GAAGGTCGGC CAGCCAGCTG TGAACAGAGC GCCTCTGCAC CGCCTGATGA GTTTGAAACC 
CACGACGACC CGTCCATCGA GTCTGAACCG TCTCCTGAGC GCCTCAGGAC AGACAAGCCG 
CCCAGCTGCC TGACGCCGCC CCACGCCTTA CATGAGTCTT TCTCCCAGCT CATCACCTCC 
CAGCACCACC CCCATGAGGT GGAGCTAACC GCTCTGAGTC TGTCAAATCT CACCATGTGT 
GATGATGCAC TGACTCTGAA GCTGCAGCCT GAGGAACCGT TCAGAGAGCT GGAGTCTGAG 
CAGACTGTTC GTCCTGCCTC AGAGAAGCTG TCGGAGGCTG GCGGTGAGAA AGGCATCCTG 
GAGCAGTCGG AGATAACACT CGTGAGCCTG ACAGACACAA CTCTGCAGGA CCAAGAGACG 
ACCATCACCG ACGAAGACGG AGTCTGGGAC GACAAACATC CAGACAGGGT GATGGAGGAA 
GGACAGAAAG AGACAGAGGA GTCGCACTCA GTGGAACAGA CTCCAGAGGA CGAGAACCAA 
ACACCTCCAG TGACCTTCCT GGAGTTTCAG TGGGGTCCGA GCCAAGACCT GCAGCAGGTC 
TACCAGCAGA AACGCCAAGC TCTGCTCCAG AGATCATCGC ACAGGGTCGA GGAAATCAAG 
TCCAGAAGAG ATGGTCCAAA GGTTCAGCCT GAGGTCCAAG CTCCGCCCAG AGAGCAGTCT 
AAAGCTAAAG ACTCTCAACC TGTCAGCTGT AAATCAAAGA CTAAGACTAA GACTGAACCT 
CAGCCTAAGA CGAACACAAA GTCAGAGGGA GGACGGGCTG AGCAACAGAC GTCTGACAAG 
TCTGGTTTCA AGCAGAAAAA GGTCCAGTGT CGACCTTCAG TGAGCGACTG CAGGCTGAAG 
ATCTGCACAC CGGAGCAGAG GAAGCTGGAT GTTACTGAGA TGCATCAGAG GACTCAGAGA 
CTGTACGAGC AGCTGGAGGA GGTGAAGCAT CAGAAAGCAG CGAGGAGCCG ACAGGAGGCG 
TCTGCAGAGA ACAGGCTGAA GGCCAAAGAG TTCCACAAGA AAACGTTACA GAAACTTCGT 
GCCAAGACGA CTCGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010753465.2
CDS	293..6013
Translation	MKRKVSQLRLSPNEEARLIREEHERRRKLRIQQVREQQRYIALQIRREVELRRRRELEQLKEELRDDWERQQREKLNTLQKLYQESLQLLGQGHRSAKENEPDLAAIAQREEENHSKAEERYREALNELKSQRLKDRERQSRSINARKKALQAEKERAAKVASLPPPPPNPIQNIDSKKPHVVKKSDVSAFAATHYHMPESTVDREADPTQPNAHEEAELEARRLQDLQREEKQRREEQLEKARLRGKQALRREHLVQDRERLLLELEHMQQTDLLRRRQQVSHMPAQIFQPLYKRQEMREDFQREMEFAFEDMYTGERRVKGDLVVQLVPEPLPALSTGSQDQDQELDITLDEIPTPAAENTHHDTEQEAGSPEQEASARAETSRPAPRRALKKLLDRIRSQRTHWSEHNGHVPAADSPTAFTNQIPEHDTTIDTGSLTSEEKDKLPEPARSPPAVSETTEPSAEAETSAPVRLQEFTDERRKRELELEQEKQQQVVLIQELEEQKAKLEQMLLEAQQEREHLKAAVTQEVPVLDQEVTSVSSGLSTELVPPPGEDEHSRRIREYQQRLLEQNRIHQRSVEVARQRLEEYQRALQIRYSMTKQSLPSVMPPGLVHPPLHSTWPVHLPAPPSLPTTPAVVAYVPARPHPSVEVTTREADMVASPPHLPGSSLRVCSRLLPDEVESVWNRAKNQKPDVSTWLTDNIMERVTEHLPDSVKPVSVTMKPPPHKLSTTRESTSILLQPTSHPIQVIGPSIADRTPLFPGHVAVKPHVSLLTGPLSSREDDMERQRQQEEEEEEERQRWELQEVQRRLLEQREAVEVQQRQKEEERQRQELQRRMQEQRAAVELQQKQQEEERQRRLLEQREAMEVQQRQTEEEKQRRELQEVQRRLLEQREAVELQKKQQEEERQRREVEMEEMRRQKETLQALIHTDAQPGAEVSSEVPLSESIGPTRRQLLASLLRAIQETNGGTLSHLEEPQEEDDSPQQPSSSYSVETDPVFQSSVPAGPLPASGLLPPPRAAKPPVTRVRLGVMKMMTEQHELSAIQEVETPVNSSLVTGLEDSVKLPSQTVGWDPQEESDSSVSSDRTLQTPSWSSSEQRPAERREASSRRYSHLDLLMEAGTSPESSESSDSVVRKTSRLSSDSGRGADYSGPAITSYRSFTESAQRPPDSDCLSSTTISSGSYITTDPEQNEPNVNTDKSLPIRRHVEQDGGANTLNVSSQSVQNFSMNSSTAARPAPDVDSLFSDSSIQRIIDRYTRELNFSLSTTGRAADSEGSHVDDPGSVSQQSLVRVSERSQCLPSDAAAAQRSVQEWDNTVDPILEHFSVEGTEQDSFRPLIGRLADQSSCLAVDHRDSTMEQLVGQPSAHSSMIGQLPGLPVGLDQGGWDSTLSRMIGRLSHQSSPHWLSGGHDFYAGQMVSERSATWLDEESRMRPLVGELDESTGHHSGSSGERTRVDPDVSTEASVPSFPALPPEASSHSASDPAVNPHPQDQNQTSPMDLGPERTEDSFHLLVAEVTHNETAEPSVNFHAVPDSPEGRPASCEQSASAPPDEFETHDDPSIESEPSPERLRTDKPPSCLTPPHALHESFSQLITSQHHPHEVELTALSLSNLTMCDDALTLKLQPEEPFRELESEQTVRPASEKLSEAGGEKGILEQSEITLVSLTDTTLQDQETTITDEDGVWDDKHPDRVMEEGQKETEESHSVEQTPEDENQTPPVTFLEFQWGPSQDLQQVYQQKRQALLQRSSHRVEEIKSRRDGPKVQPEVQAPPREQSKAKDSQPVSCKSKTKTKTEPQPKTNTKSEGGRAEQQTSDKSGFKQKKVQCRPSVSDCRLKICTPEQRKLDVTEMHQRTQRLYEQLEEVKHQKAARSRQEASAENRLKAKEFHKKTLQKLRAKTTRQ

Target ORF information:

RefSeq Version	XM_010755163.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010755163.2

ORF Insert Sequence:

ATGAAGAGGA AAGTTTCCCA GCTCAGACTG AGCCCCAACG AGGAGGCCAG GCTGATCCGA 
GAGGAGCATG AGAGGAGGAG GAAGCTGCGA ATACAGCAGG TGCGGGAGCA GCAGCGGTAC 
ATCGCGCTGC AGATCCGCCG AGAGGTCGAG TTGAGGCGGC GGCGTGAGCT CGAGCAGCTG 
AAGGAGGAGC TGAGGGACGA CTGGGAGCGA CAGCAGAGGG AGAAACTCAA CACACTGCAG 
AAACTTTACC AGGAGAGCCT CCAGCTTCTC GGCCAAGGAC ACAGGAGCGC AAAAGAAAAC 
GAACCCGACC TGGCAGCCAT AGCTCAGAGG GAGGAGGAAA ATCACAGCAA AGCAGAGGAG 
CGTTATCGAG AAGCTCTGAA TGAGCTCAAA TCACAGAGGC TTAAAGACCG TGAGAGGCAG 
AGCCGTTCCA TCAATGCCAG GAAGAAGGCG CTACAGGCAG AAAAGGAGAG AGCGGCAAAA 
GTGGCCAGCC TCCCGCCGCC GCCGCCAAAC CCCATTCAGA ACATCGATTC AAAGAAGCCT 
CATGTGGTAA AGAAGTCTGA TGTGAGCGCC TTTGCTGCTA CACATTACCA CATGCCTGAG 
AGCACAGTGG ACAGAGAGGC AGACCCAACA CAGCCTAACG CTCACGAGGA AGCTGAGCTG 
GAGGCGAGGA GACTGCAGGA CCTCCAGAGG GAGGAGAAGC AGAGGAGAGA GGAGCAGCTG 
GAGAAGGCTC GTCTCAGAGG GAAGCAGGCT CTGAGGAGGG AACACCTCGT GCAGGACCGT 
GAGCGCTTGC TCTTGGAGCT GGAACACATG CAGCAGACAG ACCTGCTGAG GAGGAGACAG 
CAGGTGTCAC ACATGCCTGC TCAGATCTTC CAGCCTCTCT ACAAGAGACA GGAGATGAGG 
GAGGACTTCC AGAGGGAGAT GGAGTTCGCC TTTGAGGACA TGTACACTGG AGAGAGGAGG 
GTTAAAGGTG ATCTGGTGGT CCAGCTGGTA CCGGAGCCTC TGCCAGCTCT GTCCACAGGC 
AGCCAAGACC AGGACCAGGA GCTGGACATC ACGCTGGACG AAATCCCCAC ACCAGCAGCA 
GAAAACACAC ACCATGACAC TGAGCAAGAA GCTGGAAGCC CTGAGCAGGA AGCATCTGCA 
CGAGCAGAGA CCTCCAGACC TGCTCCCAGA CGGGCGTTGA AGAAACTCCT GGATCGTATC 
AGGAGCCAGC GGACTCACTG GAGCGAACAC AACGGTCACG TCCCCGCAGC CGACTCGCCG 
ACCGCCTTCA CCAATCAGAT ACCAGAGCAC GACACAACCA TCGACACGGG ATCTCTGACC 
AGTGAGGAGA AGGACAAACT CCCTGAACCC GCCCGCTCTC CACCCGCAGT TTCAGAGACA 
ACAGAGCCGT CAGCTGAAGC AGAAACTTCG GCTCCTGTCC GACTCCAAGA ATTTACAGAC 
GAACGAAGGA AAAGGGAGTT GGAGCTCGAG CAGGAGAAGC AGCAGCAGGT GGTGTTGATC 
CAGGAGTTAG AGGAGCAGAA GGCCAAACTG GAGCAGATGC TGCTCGAAGC ACAGCAGGAG 
AGAGAGCACC TGAAGGCTGC TGTGACCCAG GAAGTTCCTG TCCTCGACCA GGAAGTCACC 
TCCGTCTCCT CCGGGCTGTC CACTGAGCTG GTGCCTCCTC CGGGTGAAGA TGAACACAGC 
AGAAGGATTC GAGAATACCA ACAGAGGCTG CTGGAACAGA ACAGAATTCA CCAGAGGTCG 
GTGGAGGTGG CTCGTCAGCG GCTGGAGGAA TACCAGCGAG CTCTACAGAT TCGTTACAGC 
ATGACCAAAC AGTCGCTGCC CTCCGTCATG CCTCCAGGAC TCGTTCACCC GCCGCTACAC 
AGTACTTGGC CTGTTCATCT TCCTGCTCCT CCGTCACTCC CGACAACTCC TGCAGTGGTT 
GCATACGTCC CTGCCAGACC ACACCCCTCT GTGGAAGTCA CTACAAGAGA GGCTGACATG 
GTGGCTTCAC CTCCCCACCT TCCTGGCTCC AGTTTGAGGG TTTGCTCCAG GTTGCTGCCA 
GATGAAGTGG AATCTGTTTG GAACAGAGCG AAGAACCAGA AACCCGATGT TTCTACCTGG 
CTGACTGACA ACATAATGGA GAGAGTGACT GAGCACCTTC CAGACAGCGT GAAACCCGTC 
TCAGTCACCA TGAAGCCACC GCCCCACAAA CTGTCCACAA CACGTGAGTC TACCAGCATC 
CTGCTTCAGC CGACCTCTCA TCCCATCCAA GTCATCGGCC CCAGCATTGC AGATAGAACA 
CCTCTGTTTC CAGGCCATGT AGCGGTTAAG CCGCATGTCT CCCTGCTGAC TGGGCCCCTG 
AGCTCCAGAG AGGACGACAT GGAGAGGCAG AGGCAACAAG AGGAGGAGGA GGAGGAGGAG 
AGGCAGAGAT GGGAGCTGCA GGAGGTTCAG AGACGATTGC TGGAGCAGAG GGAGGCAGTG 
GAAGTGCAAC AGAGGCAGAA AGAAGAGGAG AGGCAGAGAC AGGAGCTGCA GAGACGAATG 
CAGGAGCAAA GAGCGGCAGT AGAGCTCCAG CAGAAGCAGC AAGAAGAGGA GAGGCAGAGA 
CGGTTGCTGG AGCAGAGGGA GGCGATGGAG GTGCAACAAA GGCAGACAGA GGAGGAGAAG 
CAGAGGCGGG AGCTGCAGGA GGTTCAGAGA CGGTTGTTGG AGCAGAGGGA GGCAGTGGAG 
CTGCAGAAGA AGCAACAAGA AGAGGAGAGG CAGAGGCGGG AAGTGGAGAT GGAGGAGATG 
AGGCGACAGA AGGAGACGTT ACAGGCTCTG ATTCATACTG ACGCACAGCC AGGTGCAGAG 
GTCTCCAGTG AAGTGCCGCT TTCAGAGAGC ATCGGTCCAA CTCGACGTCA GTTACTTGCA 
TCACTGCTGC GAGCCATACA GGAAACTAAC GGAGGAACTC TATCACATCT AGAAGAGCCG 
CAGGAGGAAG ATGACTCTCC TCAACAACCA TCATCATCTT ACAGCGTCGA GACTGACCCC 
GTCTTTCAGA GCAGTGTTCC TGCTGGACCT CTCCCAGCAT CAGGCCTCCT GCCTCCTCCT 
CGAGCAGCGA AGCCTCCAGT GACTCGCGTC AGGCTGGGCG TCATGAAGAT GATGACTGAA 
CAACATGAGC TCAGTGCTAT TCAAGAGGTG GAGACGCCAG TCAACAGCAG CCTGGTCACA 
GGTCTGGAGG ACAGTGTGAA GCTTCCTTCA CAGACTGTGG GCTGGGATCC ACAGGAGGAG 
TCCGACTCGT CGGTGTCCTC TGACAGGACT CTGCAGACGC CCTCTTGGTC CAGCAGCGAG 
CAGCGGCCTG CTGAAAGACG AGAGGCGAGC TCGAGGAGGT ACAGCCATCT GGATCTGCTG 
ATGGAGGCTG GAACATCTCC AGAATCCTCA GAGTCATCTG ATTCAGTCGT GAGGAAAACG 
TCACGGCTTT CCTCTGACTC TGGCAGAGGA GCTGACTACT CCGGACCTGC GATCACGAGC 
TACAGATCCT TCACAGAGTC TGCACAGAGG CCTCCTGACT CTGACTGCCT CTCCTCCACC 
ACCATCTCCA GCGGCAGCTA CATCACCACA GACCCTGAAC AAAACGAGCC AAACGTAAAC 
ACTGATAAAT CTCTGCCCAT CAGACGTCAT GTGGAACAAG ATGGAGGAGC AAATACGCTG 
AACGTCTCCT CTCAGTCGGT TCAAAACTTC TCCATGAACA GCTCGACTGC AGCCCGTCCT 
GCTCCGGATG TCGACTCTCT GTTTAGTGAC AGCAGCATCC AGCGAATCAT CGACCGATAC 
ACGAGAGAGC TCAACTTCTC CCTCAGCACC ACCGGGAGAG CCGCAGACAG TGAAGGCTCT 
CATGTGGACG ATCCCGGCTC AGTCTCTCAG CAGTCTTTGG TTCGAGTCTC AGAGAGGAGT 
CAGTGTCTTC CCTCAGACGC AGCAGCAGCA CAGAGGAGTG TCCAGGAATG GGACAACACA 
GTCGATCCAA TCCTGGAGCA CTTCTCAGTT GAGGGCACAG AGCAGGACTC CTTCAGACCT 
CTGATTGGCC GGCTGGCAGA CCAATCATCC TGCTTGGCTG TCGACCACAG GGACTCGACC 
ATGGAGCAGC TGGTCGGTCA ACCATCGGCT CACTCGTCCA TGATCGGTCA GCTGCCCGGG 
CTGCCGGTGG GCCTCGATCA AGGCGGATGG GATTCCACTC TGAGCCGGAT GATTGGTCGA 
CTCTCCCATC AGTCCAGCCC TCATTGGCTG AGTGGAGGCC ACGACTTCTA TGCGGGTCAG 
ATGGTGTCAG AGCGATCAGC CACGTGGTTG GATGAAGAGA GCCGGATGAG ACCGCTGGTC 
GGAGAGCTGG ATGAGTCCAC TGGACACCAC AGTGGGAGCT CAGGTGAAAG AACTCGCGTG 
GATCCCGATG TTTCCACTGA GGCCAGTGTG CCGTCATTTC CAGCGTTGCC TCCTGAAGCG 
TCGTCACACA GCGCCTCTGA CCCGGCTGTA AATCCACACC CACAGGACCA GAACCAAACC 
AGTCCAATGG ACCTGGGCCC AGAGCGGACT GAAGACTCGT TCCACCTGCT CGTGGCTGAA 
GTCACCCACA ACGAAACAGC CGAGCCCTCC GTCAACTTCC ACGCTGTGCC TGACTCTCCT 
GAAGGTCGGC CAGCCAGCTG TGAACAGAGC GCCTCTGCAC CGCCTGATGA GTTTGAAACC 
CACGACGACC CGTCCATCGA GTCTGAACCG TCTCCTGAGC GCCTCAGGAC AGACAAGCCG 
CCCAGCTGCC TGACGCCGCC CCACGCCTTA CATGAGTCTT TCTCCCAGCT CATCACCTCC 
CAGCACCACC CCCATGAGGT GGAGCTAACC GCTCTGAGTC TGTCAAATCT CACCATGTGT 
GATGATGCAC TGACTCTGAA GCTGCAGCCT GAGGAACCGT TCAGAGAGCT GGAGTCTGAG 
CAGACTGTTC GTCCTGCCTC AGAGAAGCTG TCGGAGGCTG GCGGTGAGAA AGGCATCCTG 
GAGCAGTCGG AGATAACACT CGTGAGCCTG ACAGACACAA CTCTGCAGGA CCAAGAGACG 
ACCATCACCG ACGAAGACGG AGTCTGGGAC GACAAACATC CAGACAGGGT GATGGAGGAA 
GGACAGAAAG AGACAGAGGA GTCGCACTCA GTGGAACAGA CTCCAGAGGA CGAGAACCAA 
ACACCTCCAG TGACCTTCCT GGAGTTTCAG TGGGGTCCGA GCCAAGACCT GCAGCAGGTC 
TACCAGCAGA AACGCCAAGC TCTGCTCCAG AGATCATCGC ACAGGGTCGA GGAAATCAAG 
TCCAGAAGAG ATGGTCCAAA GGTTCAGCCT GAGGTCCAAG CTCCGCCCAG AGAGCAGTCT 
AAAGCTAAAG ACTCTCAACC TGTCAGCTGT AAATCAAAGA CTAAGACTAA GACTGAACCT 
CAGCCTAAGA CGAACACAAA GTCAGAGGGA GGACGGGCTG AGCAACAGAC GTCTGACAAG 
TCTGGTTTCA AGCAGAAAAA GGTCCAGTGT CGACCTTCAG TGAGCGACTG CAGGCTGAAG 
ATCTGCACAC CGGAGCAGAG GAAGCTGGAT GTTACTGAGA TGCATCAGAG GACTCAGAGA 
CTGTACGAGC AGCTGGAGGA GGTGAAGCAT CAGAAAGCAG CGAGGAGCCG ACAGGAGGCG 
TCTGCAGAGA ACAGGCTGAA GGCCAAAGAG TTCCACAAGA AAACGTTACA GAAACTTCGT 
GCCAAGACGA CTCGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep295 ( XM_019255443.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019110988.1 Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X2, mRNA.

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CloneID	OLa117479
Clone ID Related Accession (Same CDS sequence)	XM_019255443.1
Accession Version	XM_019255443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	centrosomal protein of 295 kDa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609891.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA AAGTTTCCCA GCTCAGACTG AGCCCCAACG AGGAGGCCAG GCTGATCCGA 
GAGGAGCATG AGAGGAGGAG GAAGCTGCGA ATACAGCAGG TGCGGGAGCA GCAGCGGTAC 
ATCGCGCTGC AGATCCGCCG AGAGGTCGAG TTGAGGCGGC GGCGTGAGCT CGAGCAGCTG 
AAGGAGGAGC TGAGGGACGA CTGGGAGCGA CAGCAGAGGG AGAAACTCAA CACACTGCAG 
AAACTTTACC AGGAGAGCCT CCAGCTTCTC GGCCAAGGAC ACAGGAGCGC AAAAGAAAAC 
GAACCCGACC TGGCAGCCAT AGCTCAGAGG GAGGAGGAAA ATCACAGCAA AGCAGAGGAG 
CGTTATCGAG AAGCTCTGAA TGAGCTCAAA TCACAGAGGC TTAAAGACCG TGAGAGGCAG 
AGCCGTTCCA TCAATGCCAG GAAGAAGGCG CTACAGGCAG AAAAGGAGAG AGCGGCAAAA 
GTGGCCAGCC TCCCGCCGCC GCCGCCAAAC CCCATTCAGA ACATCGATTC AAAGAAGCCT 
CATGTGGTAA AGAAGTCTGA TGTGAGCGCC TTTGCTGCTA CACATTACCA CATGCCTGAG 
AGCACAGTGG ACAGAGAGGC AGACCCAACA CAGCCTAACG CTCACGAGGA AGCTGAGCTG 
GAGGCGAGGA GACTGCAGGA CCTCCAGAGG GAGGAGAAGC AGAGGAGAGA GGAGCAGCTG 
GAGAAGGCTC GTCTCAGAGG GAAGCAGGCT CTGAGGAGGG AACACCTCGT GCAGGACCGT 
GAGCGCTTGC TCTTGGAGCT GGAACACATG CAGCAGACAG ACCTGCTGAG GAGGAGACAG 
CAGGTGTCAC ACATGCCTGC TCAGATCTTC CAGCCTCTCT ACAAGAGACA GGAGATGAGG 
GAGGACTTCC AGAGGGAGAT GGAGTTCGCC TTTGAGGACA TGTACACTGG AGAGAGGAGG 
GTTAAAGGTG ATCTGGTGGT CCAGCTGGTA CCGGAGCCTC TGCCAGCTCT GTCCACAGGC 
AGCCAAGACC AGGACCAGGA GCTGGACATC ACGCTGGACG AAATCCCCAC ACCAGCAGCA 
GAAAACACAC ACCATGACAC TGAGCAAGAA GCTGGAAGCC CTGAGCAGGA AGCATCTGCA 
CGAGCAGAGA CCTCCAGACC TGCTCCCAGA CGGGCGTTGA AGAAACTCCT GGATCGTATC 
AGGAGCCAGC GGACTCACTG GAGCGAACAC AACGGTCACG TCCCCGCAGC CGACTCGCCG 
ACCGCCTTCA CCAATCAGAT ACCAGAGCAC GACACAACCA TCGACACGGG ATCTCTGACC 
AGTGAGGAGA AGGACAAACT CCCTGAACCC GCCCGCTCTC CACCCGTTTC AGAGACAACA 
GAGCCGTCAG CTGAAGCAGA AACTTCGGCT CCTGTCCGAC TCCAAGAATT TACAGACGAA 
CGAAGGAAAA GGGAGTTGGA GCTCGAGCAG GAGAAGCAGC AGCAGGTGGT GTTGATCCAG 
GAGTTAGAGG AGCAGAAGGC CAAACTGGAG CAGATGCTGC TCGAAGCACA GCAGGAGAGA 
GAGCACCTGA AGGCTGCTGT GACCCAGGAA GTTCCTGTCC TCGACCAGGA AGTCACCTCC 
GTCTCCTCCG GGCTGTCCAC TGAGCTGGTG CCTCCTCCGG GTGAAGATGA ACACAGCAGA 
AGGATTCGAG AATACCAACA GAGGCTGCTG GAACAGAACA GAATTCACCA GAGGTCGGTG 
GAGGTGGCTC GTCAGCGGCT GGAGGAATAC CAGCGAGCTC TACAGATTCG TTACAGCATG 
ACCAAACAGT CGCTGCCCTC CGTCATGCCT CCAGGACTCG TTCACCCGCC GCTACACAGT 
ACTTGGCCTG TTCATCTTCC TGCTCCTCCG TCACTCCCGA CAACTCCTGC AGTGGTTGCA 
TACGTCCCTG CCAGACCACA CCCCTCTGTG GAAGTCACTA CAAGAGAGGC TGACATGGTG 
GCTTCACCTC CCCACCTTCC TGGCTCCAGT TTGAGGGTTT GCTCCAGGTT GCTGCCAGAT 
GAAGTGGAAT CTGTTTGGAA CAGAGCGAAG AACCAGAAAC CCGATGTTTC TACCTGGCTG 
ACTGACAACA TAATGGAGAG AGTGACTGAG CACCTTCCAG ACAGCGTGAA ACCCGTCTCA 
GTCACCATGA AGCCACCGCC CCACAAACTG TCCACAACAC GTGAGTCTAC CAGCATCCTG 
CTTCAGCCGA CCTCTCATCC CATCCAAGTC ATCGGCCCCA GCATTGCAGA TAGAACACCT 
CTGTTTCCAG GCCATGTAGC GGTTAAGCCG CATGTCTCCC TGCTGACTGG GCCCCTGAGC 
TCCAGAGAGG ACGACATGGA GAGGCAGAGG CAACAAGAGG AGGAGGAGGA GGAGGAGAGG 
CAGAGATGGG AGCTGCAGGA GGTTCAGAGA CGATTGCTGG AGCAGAGGGA GGCAGTGGAA 
GTGCAACAGA GGCAGAAAGA AGAGGAGAGG CAGAGACAGG AGCTGCAGAG ACGAATGCAG 
GAGCAAAGAG CGGCAGTAGA GCTCCAGCAG AAGCAGCAAG AAGAGGAGAG GCAGAGACGG 
TTGCTGGAGC AGAGGGAGGC GATGGAGGTG CAACAAAGGC AGACAGAGGA GGAGAAGCAG 
AGGCGGGAGC TGCAGGAGGT TCAGAGACGG TTGTTGGAGC AGAGGGAGGC AGTGGAGCTG 
CAGAAGAAGC AACAAGAAGA GGAGAGGCAG AGGCGGGAAG TGGAGATGGA GGAGATGAGG 
CGACAGAAGG AGACGTTACA GGCTCTGATT CATACTGACG CACAGCCAGG TGCAGAGGTC 
TCCAGTGAAG TGCCGCTTTC AGAGAGCATC GGTCCAACTC GACGTCAGTT ACTTGCATCA 
CTGCTGCGAG CCATACAGGA AACTAACGGA GGAACTCTAT CACATCTAGA AGAGCCGCAG 
GAGGAAGATG ACTCTCCTCA ACAACCATCA TCATCTTACA GCGTCGAGAC TGACCCCGTC 
TTTCAGAGCA GTGTTCCTGC TGGACCTCTC CCAGCATCAG GCCTCCTGCC TCCTCCTCGA 
GCAGCGAAGC CTCCAGTGAC TCGCGTCAGG CTGGGCGTCA TGAAGATGAT GACTGAACAA 
CATGAGCTCA GTGCTATTCA AGAGGTGGAG ACGCCAGTCA ACAGCAGCCT GGTCACAGGT 
CTGGAGGACA GTGTGAAGCT TCCTTCACAG ACTGTGGGCT GGGATCCACA GGAGGAGTCC 
GACTCGTCGG TGTCCTCTGA CAGGACTCTG CAGACGCCCT CTTGGTCCAG CAGCGAGCAG 
CGGCCTGCTG AAAGACGAGA GGCGAGCTCG AGGAGGTACA GCCATCTGGA TCTGCTGATG 
GAGGCTGGAA CATCTCCAGA ATCCTCAGAG TCATCTGATT CAGTCGTGAG GAAAACGTCA 
CGGCTTTCCT CTGACTCTGG CAGAGGAGCT GACTACTCCG GACCTGCGAT CACGAGCTAC 
AGATCCTTCA CAGAGTCTGC ACAGAGGCCT CCTGACTCTG ACTGCCTCTC CTCCACCACC 
ATCTCCAGCG GCAGCTACAT CACCACAGAC CCTGAACAAA ACGAGCCAAA CGTAAACACT 
GATAAATCTC TGCCCATCAG ACGTCATGTG GAACAAGATG GAGGAGCAAA TACGCTGAAC 
GTCTCCTCTC AGTCGGTTCA AAACTTCTCC ATGAACAGCT CGACTGCAGC CCGTCCTGCT 
CCGGATGTCG ACTCTCTGTT TAGTGACAGC AGCATCCAGC GAATCATCGA CCGATACACG 
AGAGAGCTCA ACTTCTCCCT CAGCACCACC GGGAGAGCCG CAGACAGTGA AGGCTCTCAT 
GTGGACGATC CCGGCTCAGT CTCTCAGCAG TCTTTGGTTC GAGTCTCAGA GAGGAGTCAG 
TGTCTTCCCT CAGACGCAGC AGCAGCACAG AGGAGTGTCC AGGAATGGGA CAACACAGTC 
GATCCAATCC TGGAGCACTT CTCAGTTGAG GGCACAGAGC AGGACTCCTT CAGACCTCTG 
ATTGGCCGGC TGGCAGACCA ATCATCCTGC TTGGCTGTCG ACCACAGGGA CTCGACCATG 
GAGCAGCTGG TCGGTCAACC ATCGGCTCAC TCGTCCATGA TCGGTCAGCT GCCCGGGCTG 
CCGGTGGGCC TCGATCAAGG CGGATGGGAT TCCACTCTGA GCCGGATGAT TGGTCGACTC 
TCCCATCAGT CCAGCCCTCA TTGGCTGAGT GGAGGCCACG ACTTCTATGC GGGTCAGATG 
GTGTCAGAGC GATCAGCCAC GTGGTTGGAT GAAGAGAGCC GGATGAGACC GCTGGTCGGA 
GAGCTGGATG AGTCCACTGG ACACCACAGT GGGAGCTCAG GTGAAAGAAC TCGCGTGGAT 
CCCGATGTTT CCACTGAGGC CAGTGTGCCG TCATTTCCAG CGTTGCCTCC TGAAGCGTCG 
TCACACAGCG CCTCTGACCC GGCTGTAAAT CCACACCCAC AGGACCAGAA CCAAACCAGT 
CCAATGGACC TGGGCCCAGA GCGGACTGAA GACTCGTTCC ACCTGCTCGT GGCTGAAGTC 
ACCCACAACG AAACAGCCGA GCCCTCCGTC AACTTCCACG CTGTGCCTGA CTCTCCTGAA 
GGTCGGCCAG CCAGCTGTGA ACAGAGCGCC TCTGCACCGC CTGATGAGTT TGAAACCCAC 
GACGACCCGT CCATCGAGTC TGAACCGTCT CCTGAGCGCC TCAGGACAGA CAAGCCGCCC 
AGCTGCCTGA CGCCGCCCCA CGCCTTACAT GAGTCTTTCT CCCAGCTCAT CACCTCCCAG 
CACCACCCCC ATGAGGTGGA GCTAACCGCT CTGAGTCTGT CAAATCTCAC CATGTGTGAT 
GATGCACTGA CTCTGAAGCT GCAGCCTGAG GAACCGTTCA GAGAGCTGGA GTCTGAGCAG 
ACTGTTCGTC CTGCCTCAGA GAAGCTGTCG GAGGCTGGCG GTGAGAAAGG CATCCTGGAG 
CAGTCGGAGA TAACACTCGT GAGCCTGACA GACACAACTC TGCAGGACCA AGAGACGACC 
ATCACCGACG AAGACGGAGT CTGGGACGAC AAACATCCAG ACAGGGTGAT GGAGGAAGGA 
CAGAAAGAGA CAGAGGAGTC GCACTCAGTG GAACAGACTC CAGAGGACGA GAACCAAACA 
CCTCCAGTGA CCTTCCTGGA GTTTCAGTGG GGTCCGAGCC AAGACCTGCA GCAGGTCTAC 
CAGCAGAAAC GCCAAGCTCT GCTCCAGAGA TCATCGCACA GGGTCGAGGA AATCAAGTCC 
AGAAGAGATG GTCCAAAGGT TCAGCCTGAG GTCCAAGCTC CGCCCAGAGA GCAGTCTAAA 
GCTAAAGACT CTCAACCTGT CAGCTGTAAA TCAAAGACTA AGACTAAGAC TGAACCTCAG 
CCTAAGACGA ACACAAAGTC AGAGGGAGGA CGGGCTGAGC AACAGACGTC TGACAAGTCT 
GGTTTCAAGC AGAAAAAGGT CCAGTGTCGA CCTTCAGTGA GCGACTGCAG GCTGAAGATC 
TGCACACCGG AGCAGAGGAA GCTGGATGTT ACTGAGATGC ATCAGAGGAC TCAGAGACTG 
TACGAGCAGC TGGAGGAGGT GAAGCATCAG AAAGCAGCGA GGAGCCGACA GGAGGCGTCT 
GCAGAGAACA GGCTGAAGGC CAAAGAGTTC CACAAGAAAA CGTTACAGAA ACTTCGTGCC 
AAGACGACTC GGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019110988.1
CDS	293..6010
Translation	MKRKVSQLRLSPNEEARLIREEHERRRKLRIQQVREQQRYIALQIRREVELRRRRELEQLKEELRDDWERQQREKLNTLQKLYQESLQLLGQGHRSAKENEPDLAAIAQREEENHSKAEERYREALNELKSQRLKDRERQSRSINARKKALQAEKERAAKVASLPPPPPNPIQNIDSKKPHVVKKSDVSAFAATHYHMPESTVDREADPTQPNAHEEAELEARRLQDLQREEKQRREEQLEKARLRGKQALRREHLVQDRERLLLELEHMQQTDLLRRRQQVSHMPAQIFQPLYKRQEMREDFQREMEFAFEDMYTGERRVKGDLVVQLVPEPLPALSTGSQDQDQELDITLDEIPTPAAENTHHDTEQEAGSPEQEASARAETSRPAPRRALKKLLDRIRSQRTHWSEHNGHVPAADSPTAFTNQIPEHDTTIDTGSLTSEEKDKLPEPARSPPVSETTEPSAEAETSAPVRLQEFTDERRKRELELEQEKQQQVVLIQELEEQKAKLEQMLLEAQQEREHLKAAVTQEVPVLDQEVTSVSSGLSTELVPPPGEDEHSRRIREYQQRLLEQNRIHQRSVEVARQRLEEYQRALQIRYSMTKQSLPSVMPPGLVHPPLHSTWPVHLPAPPSLPTTPAVVAYVPARPHPSVEVTTREADMVASPPHLPGSSLRVCSRLLPDEVESVWNRAKNQKPDVSTWLTDNIMERVTEHLPDSVKPVSVTMKPPPHKLSTTRESTSILLQPTSHPIQVIGPSIADRTPLFPGHVAVKPHVSLLTGPLSSREDDMERQRQQEEEEEEERQRWELQEVQRRLLEQREAVEVQQRQKEEERQRQELQRRMQEQRAAVELQQKQQEEERQRRLLEQREAMEVQQRQTEEEKQRRELQEVQRRLLEQREAVELQKKQQEEERQRREVEMEEMRRQKETLQALIHTDAQPGAEVSSEVPLSESIGPTRRQLLASLLRAIQETNGGTLSHLEEPQEEDDSPQQPSSSYSVETDPVFQSSVPAGPLPASGLLPPPRAAKPPVTRVRLGVMKMMTEQHELSAIQEVETPVNSSLVTGLEDSVKLPSQTVGWDPQEESDSSVSSDRTLQTPSWSSSEQRPAERREASSRRYSHLDLLMEAGTSPESSESSDSVVRKTSRLSSDSGRGADYSGPAITSYRSFTESAQRPPDSDCLSSTTISSGSYITTDPEQNEPNVNTDKSLPIRRHVEQDGGANTLNVSSQSVQNFSMNSSTAARPAPDVDSLFSDSSIQRIIDRYTRELNFSLSTTGRAADSEGSHVDDPGSVSQQSLVRVSERSQCLPSDAAAAQRSVQEWDNTVDPILEHFSVEGTEQDSFRPLIGRLADQSSCLAVDHRDSTMEQLVGQPSAHSSMIGQLPGLPVGLDQGGWDSTLSRMIGRLSHQSSPHWLSGGHDFYAGQMVSERSATWLDEESRMRPLVGELDESTGHHSGSSGERTRVDPDVSTEASVPSFPALPPEASSHSASDPAVNPHPQDQNQTSPMDLGPERTEDSFHLLVAEVTHNETAEPSVNFHAVPDSPEGRPASCEQSASAPPDEFETHDDPSIESEPSPERLRTDKPPSCLTPPHALHESFSQLITSQHHPHEVELTALSLSNLTMCDDALTLKLQPEEPFRELESEQTVRPASEKLSEAGGEKGILEQSEITLVSLTDTTLQDQETTITDEDGVWDDKHPDRVMEEGQKETEESHSVEQTPEDENQTPPVTFLEFQWGPSQDLQQVYQQKRQALLQRSSHRVEEIKSRRDGPKVQPEVQAPPREQSKAKDSQPVSCKSKTKTKTEPQPKTNTKSEGGRAEQQTSDKSGFKQKKVQCRPSVSDCRLKICTPEQRKLDVTEMHQRTQRLYEQLEEVKHQKAARSRQEASAENRLKAKEFHKKTLQKLRAKTTRQ

Target ORF information:

RefSeq Version	XM_019255443.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019255443.1

ORF Insert Sequence:

ATGAAGAGGA AAGTTTCCCA GCTCAGACTG AGCCCCAACG AGGAGGCCAG GCTGATCCGA 
GAGGAGCATG AGAGGAGGAG GAAGCTGCGA ATACAGCAGG TGCGGGAGCA GCAGCGGTAC 
ATCGCGCTGC AGATCCGCCG AGAGGTCGAG TTGAGGCGGC GGCGTGAGCT CGAGCAGCTG 
AAGGAGGAGC TGAGGGACGA CTGGGAGCGA CAGCAGAGGG AGAAACTCAA CACACTGCAG 
AAACTTTACC AGGAGAGCCT CCAGCTTCTC GGCCAAGGAC ACAGGAGCGC AAAAGAAAAC 
GAACCCGACC TGGCAGCCAT AGCTCAGAGG GAGGAGGAAA ATCACAGCAA AGCAGAGGAG 
CGTTATCGAG AAGCTCTGAA TGAGCTCAAA TCACAGAGGC TTAAAGACCG TGAGAGGCAG 
AGCCGTTCCA TCAATGCCAG GAAGAAGGCG CTACAGGCAG AAAAGGAGAG AGCGGCAAAA 
GTGGCCAGCC TCCCGCCGCC GCCGCCAAAC CCCATTCAGA ACATCGATTC AAAGAAGCCT 
CATGTGGTAA AGAAGTCTGA TGTGAGCGCC TTTGCTGCTA CACATTACCA CATGCCTGAG 
AGCACAGTGG ACAGAGAGGC AGACCCAACA CAGCCTAACG CTCACGAGGA AGCTGAGCTG 
GAGGCGAGGA GACTGCAGGA CCTCCAGAGG GAGGAGAAGC AGAGGAGAGA GGAGCAGCTG 
GAGAAGGCTC GTCTCAGAGG GAAGCAGGCT CTGAGGAGGG AACACCTCGT GCAGGACCGT 
GAGCGCTTGC TCTTGGAGCT GGAACACATG CAGCAGACAG ACCTGCTGAG GAGGAGACAG 
CAGGTGTCAC ACATGCCTGC TCAGATCTTC CAGCCTCTCT ACAAGAGACA GGAGATGAGG 
GAGGACTTCC AGAGGGAGAT GGAGTTCGCC TTTGAGGACA TGTACACTGG AGAGAGGAGG 
GTTAAAGGTG ATCTGGTGGT CCAGCTGGTA CCGGAGCCTC TGCCAGCTCT GTCCACAGGC 
AGCCAAGACC AGGACCAGGA GCTGGACATC ACGCTGGACG AAATCCCCAC ACCAGCAGCA 
GAAAACACAC ACCATGACAC TGAGCAAGAA GCTGGAAGCC CTGAGCAGGA AGCATCTGCA 
CGAGCAGAGA CCTCCAGACC TGCTCCCAGA CGGGCGTTGA AGAAACTCCT GGATCGTATC 
AGGAGCCAGC GGACTCACTG GAGCGAACAC AACGGTCACG TCCCCGCAGC CGACTCGCCG 
ACCGCCTTCA CCAATCAGAT ACCAGAGCAC GACACAACCA TCGACACGGG ATCTCTGACC 
AGTGAGGAGA AGGACAAACT CCCTGAACCC GCCCGCTCTC CACCCGTTTC AGAGACAACA 
GAGCCGTCAG CTGAAGCAGA AACTTCGGCT CCTGTCCGAC TCCAAGAATT TACAGACGAA 
CGAAGGAAAA GGGAGTTGGA GCTCGAGCAG GAGAAGCAGC AGCAGGTGGT GTTGATCCAG 
GAGTTAGAGG AGCAGAAGGC CAAACTGGAG CAGATGCTGC TCGAAGCACA GCAGGAGAGA 
GAGCACCTGA AGGCTGCTGT GACCCAGGAA GTTCCTGTCC TCGACCAGGA AGTCACCTCC 
GTCTCCTCCG GGCTGTCCAC TGAGCTGGTG CCTCCTCCGG GTGAAGATGA ACACAGCAGA 
AGGATTCGAG AATACCAACA GAGGCTGCTG GAACAGAACA GAATTCACCA GAGGTCGGTG 
GAGGTGGCTC GTCAGCGGCT GGAGGAATAC CAGCGAGCTC TACAGATTCG TTACAGCATG 
ACCAAACAGT CGCTGCCCTC CGTCATGCCT CCAGGACTCG TTCACCCGCC GCTACACAGT 
ACTTGGCCTG TTCATCTTCC TGCTCCTCCG TCACTCCCGA CAACTCCTGC AGTGGTTGCA 
TACGTCCCTG CCAGACCACA CCCCTCTGTG GAAGTCACTA CAAGAGAGGC TGACATGGTG 
GCTTCACCTC CCCACCTTCC TGGCTCCAGT TTGAGGGTTT GCTCCAGGTT GCTGCCAGAT 
GAAGTGGAAT CTGTTTGGAA CAGAGCGAAG AACCAGAAAC CCGATGTTTC TACCTGGCTG 
ACTGACAACA TAATGGAGAG AGTGACTGAG CACCTTCCAG ACAGCGTGAA ACCCGTCTCA 
GTCACCATGA AGCCACCGCC CCACAAACTG TCCACAACAC GTGAGTCTAC CAGCATCCTG 
CTTCAGCCGA CCTCTCATCC CATCCAAGTC ATCGGCCCCA GCATTGCAGA TAGAACACCT 
CTGTTTCCAG GCCATGTAGC GGTTAAGCCG CATGTCTCCC TGCTGACTGG GCCCCTGAGC 
TCCAGAGAGG ACGACATGGA GAGGCAGAGG CAACAAGAGG AGGAGGAGGA GGAGGAGAGG 
CAGAGATGGG AGCTGCAGGA GGTTCAGAGA CGATTGCTGG AGCAGAGGGA GGCAGTGGAA 
GTGCAACAGA GGCAGAAAGA AGAGGAGAGG CAGAGACAGG AGCTGCAGAG ACGAATGCAG 
GAGCAAAGAG CGGCAGTAGA GCTCCAGCAG AAGCAGCAAG AAGAGGAGAG GCAGAGACGG 
TTGCTGGAGC AGAGGGAGGC GATGGAGGTG CAACAAAGGC AGACAGAGGA GGAGAAGCAG 
AGGCGGGAGC TGCAGGAGGT TCAGAGACGG TTGTTGGAGC AGAGGGAGGC AGTGGAGCTG 
CAGAAGAAGC AACAAGAAGA GGAGAGGCAG AGGCGGGAAG TGGAGATGGA GGAGATGAGG 
CGACAGAAGG AGACGTTACA GGCTCTGATT CATACTGACG CACAGCCAGG TGCAGAGGTC 
TCCAGTGAAG TGCCGCTTTC AGAGAGCATC GGTCCAACTC GACGTCAGTT ACTTGCATCA 
CTGCTGCGAG CCATACAGGA AACTAACGGA GGAACTCTAT CACATCTAGA AGAGCCGCAG 
GAGGAAGATG ACTCTCCTCA ACAACCATCA TCATCTTACA GCGTCGAGAC TGACCCCGTC 
TTTCAGAGCA GTGTTCCTGC TGGACCTCTC CCAGCATCAG GCCTCCTGCC TCCTCCTCGA 
GCAGCGAAGC CTCCAGTGAC TCGCGTCAGG CTGGGCGTCA TGAAGATGAT GACTGAACAA 
CATGAGCTCA GTGCTATTCA AGAGGTGGAG ACGCCAGTCA ACAGCAGCCT GGTCACAGGT 
CTGGAGGACA GTGTGAAGCT TCCTTCACAG ACTGTGGGCT GGGATCCACA GGAGGAGTCC 
GACTCGTCGG TGTCCTCTGA CAGGACTCTG CAGACGCCCT CTTGGTCCAG CAGCGAGCAG 
CGGCCTGCTG AAAGACGAGA GGCGAGCTCG AGGAGGTACA GCCATCTGGA TCTGCTGATG 
GAGGCTGGAA CATCTCCAGA ATCCTCAGAG TCATCTGATT CAGTCGTGAG GAAAACGTCA 
CGGCTTTCCT CTGACTCTGG CAGAGGAGCT GACTACTCCG GACCTGCGAT CACGAGCTAC 
AGATCCTTCA CAGAGTCTGC ACAGAGGCCT CCTGACTCTG ACTGCCTCTC CTCCACCACC 
ATCTCCAGCG GCAGCTACAT CACCACAGAC CCTGAACAAA ACGAGCCAAA CGTAAACACT 
GATAAATCTC TGCCCATCAG ACGTCATGTG GAACAAGATG GAGGAGCAAA TACGCTGAAC 
GTCTCCTCTC AGTCGGTTCA AAACTTCTCC ATGAACAGCT CGACTGCAGC CCGTCCTGCT 
CCGGATGTCG ACTCTCTGTT TAGTGACAGC AGCATCCAGC GAATCATCGA CCGATACACG 
AGAGAGCTCA ACTTCTCCCT CAGCACCACC GGGAGAGCCG CAGACAGTGA AGGCTCTCAT 
GTGGACGATC CCGGCTCAGT CTCTCAGCAG TCTTTGGTTC GAGTCTCAGA GAGGAGTCAG 
TGTCTTCCCT CAGACGCAGC AGCAGCACAG AGGAGTGTCC AGGAATGGGA CAACACAGTC 
GATCCAATCC TGGAGCACTT CTCAGTTGAG GGCACAGAGC AGGACTCCTT CAGACCTCTG 
ATTGGCCGGC TGGCAGACCA ATCATCCTGC TTGGCTGTCG ACCACAGGGA CTCGACCATG 
GAGCAGCTGG TCGGTCAACC ATCGGCTCAC TCGTCCATGA TCGGTCAGCT GCCCGGGCTG 
CCGGTGGGCC TCGATCAAGG CGGATGGGAT TCCACTCTGA GCCGGATGAT TGGTCGACTC 
TCCCATCAGT CCAGCCCTCA TTGGCTGAGT GGAGGCCACG ACTTCTATGC GGGTCAGATG 
GTGTCAGAGC GATCAGCCAC GTGGTTGGAT GAAGAGAGCC GGATGAGACC GCTGGTCGGA 
GAGCTGGATG AGTCCACTGG ACACCACAGT GGGAGCTCAG GTGAAAGAAC TCGCGTGGAT 
CCCGATGTTT CCACTGAGGC CAGTGTGCCG TCATTTCCAG CGTTGCCTCC TGAAGCGTCG 
TCACACAGCG CCTCTGACCC GGCTGTAAAT CCACACCCAC AGGACCAGAA CCAAACCAGT 
CCAATGGACC TGGGCCCAGA GCGGACTGAA GACTCGTTCC ACCTGCTCGT GGCTGAAGTC 
ACCCACAACG AAACAGCCGA GCCCTCCGTC AACTTCCACG CTGTGCCTGA CTCTCCTGAA 
GGTCGGCCAG CCAGCTGTGA ACAGAGCGCC TCTGCACCGC CTGATGAGTT TGAAACCCAC 
GACGACCCGT CCATCGAGTC TGAACCGTCT CCTGAGCGCC TCAGGACAGA CAAGCCGCCC 
AGCTGCCTGA CGCCGCCCCA CGCCTTACAT GAGTCTTTCT CCCAGCTCAT CACCTCCCAG 
CACCACCCCC ATGAGGTGGA GCTAACCGCT CTGAGTCTGT CAAATCTCAC CATGTGTGAT 
GATGCACTGA CTCTGAAGCT GCAGCCTGAG GAACCGTTCA GAGAGCTGGA GTCTGAGCAG 
ACTGTTCGTC CTGCCTCAGA GAAGCTGTCG GAGGCTGGCG GTGAGAAAGG CATCCTGGAG 
CAGTCGGAGA TAACACTCGT GAGCCTGACA GACACAACTC TGCAGGACCA AGAGACGACC 
ATCACCGACG AAGACGGAGT CTGGGACGAC AAACATCCAG ACAGGGTGAT GGAGGAAGGA 
CAGAAAGAGA CAGAGGAGTC GCACTCAGTG GAACAGACTC CAGAGGACGA GAACCAAACA 
CCTCCAGTGA CCTTCCTGGA GTTTCAGTGG GGTCCGAGCC AAGACCTGCA GCAGGTCTAC 
CAGCAGAAAC GCCAAGCTCT GCTCCAGAGA TCATCGCACA GGGTCGAGGA AATCAAGTCC 
AGAAGAGATG GTCCAAAGGT TCAGCCTGAG GTCCAAGCTC CGCCCAGAGA GCAGTCTAAA 
GCTAAAGACT CTCAACCTGT CAGCTGTAAA TCAAAGACTA AGACTAAGAC TGAACCTCAG 
CCTAAGACGA ACACAAAGTC AGAGGGAGGA CGGGCTGAGC AACAGACGTC TGACAAGTCT 
GGTTTCAAGC AGAAAAAGGT CCAGTGTCGA CCTTCAGTGA GCGACTGCAG GCTGAAGATC 
TGCACACCGG AGCAGAGGAA GCTGGATGTT ACTGAGATGC ATCAGAGGAC TCAGAGACTG 
TACGAGCAGC TGGAGGAGGT GAAGCATCAG AAAGCAGCGA GGAGCCGACA GGAGGCGTCT 
GCAGAGAACA GGCTGAAGGC CAAAGAGTTC CACAAGAAAA CGTTACAGAA ACTTCGTGCC 
AAGACGACTC GGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep295 ( XM_019255444.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019110989.1 Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X3, mRNA.

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CloneID	OLa117480
Clone ID Related Accession (Same CDS sequence)	XM_019255444.1
Accession Version	XM_019255444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	centrosomal protein of 295 kDa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609891.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA AAGTTTCCCA GCTCAGACTG AGCCCCAACG AGGAGGCCAG GCTGATCCGA 
GAGGAGCATG AGAGGAGGAG GAAGCTGCGA ATACAGCAGG TGCGGGAGCA GCAGCGGTAC 
ATCGCGCTGC AGATCCGCCG AGAGGTCGAG TTGAGGCGGC GGCGTGAGCT CGAGCAGCTG 
AAGGAGGAGC TGAGGGACGA CTGGGAGCGA CAGCAGAGGG AGAAACTCAA CACACTGCAG 
AAACTTTACC AGGAGAGCCT CCAGCTTCTC GGCCAAGGAC ACAGGAGCGC AAAAGAAAAC 
GAACCCGACC TGGCAGCCAT AGCTCAGAGG GAGGAGGAAA ATCACAGCAA AGCAGAGGAG 
CGTTATCGAG AAGCTCTGAA TGAGCTCAAA TCACAGAGGC TTAAAGACCG TGAGAGGCAG 
AGCCGTTCCA TCAATGCCAG GAAGAAGGCG CTACAGGCAG AAAAGGAGAG AGCGGCAAAA 
GTGGCCAGCC TCCCGCCGCC GCCGCCAAAC CCCATTCAGA ACATCGATTC AAAGAAGCCT 
CATGTGGTAA AGAAGTCTGA TGTGAGCGCC TTTGCTGCTA CACATTACCA CATGCCTGAG 
AGCACAGTGG ACAGAGAGGC AGACCCAACA CAGCCTAACG CTCACGAGGA AGCTGAGCTG 
GAGGCGAGGA GACTGCAGGA CCTCCAGAGG GAGGAGAAGC AGAGGAGAGA GGAGCAGCTG 
GAGAAGGCTC GTCTCAGAGG GAAGCAGGCT CTGAGGAGGG AACACCTCGT GCAGGACCGT 
GAGCGCTTGC TCTTGGAGCT GGAACACATG CAGCAGACAG ACCTGCTGAG GAGGAGACAG 
CAGGTGTCAC ACATGCCTGC TCAGATCTTC CAGCCTCTCT ACAAGAGACA GGAGATGAGG 
GAGGACTTCC AGAGGGAGAT GGAGTTCGCC TTTGAGGACA TGTACACTGG AGAGAGGAGG 
GTTAAAGGTG ATCTGGTGGT CCAGCTGGTA CCGGAGCCTC TGCCAGCTCT GTCCACAGGC 
AGCCAAGACC AGGACCAGGA GCTGGACATC ACGCTGGACG AAATCCCCAC ACCAGCAGCA 
GAAAACACAC ACCATGACAC TGAGCAAGAA GCTGGAAGCC CTGAGCAGGA AGCATCTGCA 
CGAGAGACCT CCAGACCTGC TCCCAGACGG GCGTTGAAGA AACTCCTGGA TCGTATCAGG 
AGCCAGCGGA CTCACTGGAG CGAACACAAC GGTCACGTCC CCGCAGCCGA CTCGCCGACC 
GCCTTCACCA ATCAGATACC AGAGCACGAC ACAACCATCG ACACGGGATC TCTGACCAGT 
GAGGAGAAGG ACAAACTCCC TGAACCCGCC CGCTCTCCAC CCGCAGTTTC AGAGACAACA 
GAGCCGTCAG CTGAAGCAGA AACTTCGGCT CCTGTCCGAC TCCAAGAATT TACAGACGAA 
CGAAGGAAAA GGGAGTTGGA GCTCGAGCAG GAGAAGCAGC AGCAGGTGGT GTTGATCCAG 
GAGTTAGAGG AGCAGAAGGC CAAACTGGAG CAGATGCTGC TCGAAGCACA GCAGGAGAGA 
GAGCACCTGA AGGCTGCTGT GACCCAGGAA GTTCCTGTCC TCGACCAGGA AGTCACCTCC 
GTCTCCTCCG GGCTGTCCAC TGAGCTGGTG CCTCCTCCGG GTGAAGATGA ACACAGCAGA 
AGGATTCGAG AATACCAACA GAGGCTGCTG GAACAGAACA GAATTCACCA GAGGTCGGTG 
GAGGTGGCTC GTCAGCGGCT GGAGGAATAC CAGCGAGCTC TACAGATTCG TTACAGCATG 
ACCAAACAGT CGCTGCCCTC CGTCATGCCT CCAGGACTCG TTCACCCGCC GCTACACAGT 
ACTTGGCCTG TTCATCTTCC TGCTCCTCCG TCACTCCCGA CAACTCCTGC AGTGGTTGCA 
TACGTCCCTG CCAGACCACA CCCCTCTGTG GAAGTCACTA CAAGAGAGGC TGACATGGTG 
GCTTCACCTC CCCACCTTCC TGGCTCCAGT TTGAGGGTTT GCTCCAGGTT GCTGCCAGAT 
GAAGTGGAAT CTGTTTGGAA CAGAGCGAAG AACCAGAAAC CCGATGTTTC TACCTGGCTG 
ACTGACAACA TAATGGAGAG AGTGACTGAG CACCTTCCAG ACAGCGTGAA ACCCGTCTCA 
GTCACCATGA AGCCACCGCC CCACAAACTG TCCACAACAC GTGAGTCTAC CAGCATCCTG 
CTTCAGCCGA CCTCTCATCC CATCCAAGTC ATCGGCCCCA GCATTGCAGA TAGAACACCT 
CTGTTTCCAG GCCATGTAGC GGTTAAGCCG CATGTCTCCC TGCTGACTGG GCCCCTGAGC 
TCCAGAGAGG ACGACATGGA GAGGCAGAGG CAACAAGAGG AGGAGGAGGA GGAGGAGAGG 
CAGAGATGGG AGCTGCAGGA GGTTCAGAGA CGATTGCTGG AGCAGAGGGA GGCAGTGGAA 
GTGCAACAGA GGCAGAAAGA AGAGGAGAGG CAGAGACAGG AGCTGCAGAG ACGAATGCAG 
GAGCAAAGAG CGGCAGTAGA GCTCCAGCAG AAGCAGCAAG AAGAGGAGAG GCAGAGACGG 
TTGCTGGAGC AGAGGGAGGC GATGGAGGTG CAACAAAGGC AGACAGAGGA GGAGAAGCAG 
AGGCGGGAGC TGCAGGAGGT TCAGAGACGG TTGTTGGAGC AGAGGGAGGC AGTGGAGCTG 
CAGAAGAAGC AACAAGAAGA GGAGAGGCAG AGGCGGGAAG TGGAGATGGA GGAGATGAGG 
CGACAGAAGG AGACGTTACA GGCTCTGATT CATACTGACG CACAGCCAGG TGCAGAGGTC 
TCCAGTGAAG TGCCGCTTTC AGAGAGCATC GGTCCAACTC GACGTCAGTT ACTTGCATCA 
CTGCTGCGAG CCATACAGGA AACTAACGGA GGAACTCTAT CACATCTAGA AGAGCCGCAG 
GAGGAAGATG ACTCTCCTCA ACAACCATCA TCATCTTACA GCGTCGAGAC TGACCCCGTC 
TTTCAGAGCA GTGTTCCTGC TGGACCTCTC CCAGCATCAG GCCTCCTGCC TCCTCCTCGA 
GCAGCGAAGC CTCCAGTGAC TCGCGTCAGG CTGGGCGTCA TGAAGATGAT GACTGAACAA 
CATGAGCTCA GTGCTATTCA AGAGGTGGAG ACGCCAGTCA ACAGCAGCCT GGTCACAGGT 
CTGGAGGACA GTGTGAAGCT TCCTTCACAG ACTGTGGGCT GGGATCCACA GGAGGAGTCC 
GACTCGTCGG TGTCCTCTGA CAGGACTCTG CAGACGCCCT CTTGGTCCAG CAGCGAGCAG 
CGGCCTGCTG AAAGACGAGA GGCGAGCTCG AGGAGGTACA GCCATCTGGA TCTGCTGATG 
GAGGCTGGAA CATCTCCAGA ATCCTCAGAG TCATCTGATT CAGTCGTGAG GAAAACGTCA 
CGGCTTTCCT CTGACTCTGG CAGAGGAGCT GACTACTCCG GACCTGCGAT CACGAGCTAC 
AGATCCTTCA CAGAGTCTGC ACAGAGGCCT CCTGACTCTG ACTGCCTCTC CTCCACCACC 
ATCTCCAGCG GCAGCTACAT CACCACAGAC CCTGAACAAA ACGAGCCAAA CGTAAACACT 
GATAAATCTC TGCCCATCAG ACGTCATGTG GAACAAGATG GAGGAGCAAA TACGCTGAAC 
GTCTCCTCTC AGTCGGTTCA AAACTTCTCC ATGAACAGCT CGACTGCAGC CCGTCCTGCT 
CCGGATGTCG ACTCTCTGTT TAGTGACAGC AGCATCCAGC GAATCATCGA CCGATACACG 
AGAGAGCTCA ACTTCTCCCT CAGCACCACC GGGAGAGCCG CAGACAGTGA AGGCTCTCAT 
GTGGACGATC CCGGCTCAGT CTCTCAGCAG TCTTTGGTTC GAGTCTCAGA GAGGAGTCAG 
TGTCTTCCCT CAGACGCAGC AGCAGCACAG AGGAGTGTCC AGGAATGGGA CAACACAGTC 
GATCCAATCC TGGAGCACTT CTCAGTTGAG GGCACAGAGC AGGACTCCTT CAGACCTCTG 
ATTGGCCGGC TGGCAGACCA ATCATCCTGC TTGGCTGTCG ACCACAGGGA CTCGACCATG 
GAGCAGCTGG TCGGTCAACC ATCGGCTCAC TCGTCCATGA TCGGTCAGCT GCCCGGGCTG 
CCGGTGGGCC TCGATCAAGG CGGATGGGAT TCCACTCTGA GCCGGATGAT TGGTCGACTC 
TCCCATCAGT CCAGCCCTCA TTGGCTGAGT GGAGGCCACG ACTTCTATGC GGGTCAGATG 
GTGTCAGAGC GATCAGCCAC GTGGTTGGAT GAAGAGAGCC GGATGAGACC GCTGGTCGGA 
GAGCTGGATG AGTCCACTGG ACACCACAGT GGGAGCTCAG GTGAAAGAAC TCGCGTGGAT 
CCCGATGTTT CCACTGAGGC CAGTGTGCCG TCATTTCCAG CGTTGCCTCC TGAAGCGTCG 
TCACACAGCG CCTCTGACCC GGCTGTAAAT CCACACCCAC AGGACCAGAA CCAAACCAGT 
CCAATGGACC TGGGCCCAGA GCGGACTGAA GACTCGTTCC ACCTGCTCGT GGCTGAAGTC 
ACCCACAACG AAACAGCCGA GCCCTCCGTC AACTTCCACG CTGTGCCTGA CTCTCCTGAA 
GGTCGGCCAG CCAGCTGTGA ACAGAGCGCC TCTGCACCGC CTGATGAGTT TGAAACCCAC 
GACGACCCGT CCATCGAGTC TGAACCGTCT CCTGAGCGCC TCAGGACAGA CAAGCCGCCC 
AGCTGCCTGA CGCCGCCCCA CGCCTTACAT GAGTCTTTCT CCCAGCTCAT CACCTCCCAG 
CACCACCCCC ATGAGGTGGA GCTAACCGCT CTGAGTCTGT CAAATCTCAC CATGTGTGAT 
GATGCACTGA CTCTGAAGCT GCAGCCTGAG GAACCGTTCA GAGAGCTGGA GTCTGAGCAG 
ACTGTTCGTC CTGCCTCAGA GAAGCTGTCG GAGGCTGGCG GTGAGAAAGG CATCCTGGAG 
CAGTCGGAGA TAACACTCGT GAGCCTGACA GACACAACTC TGCAGGACCA AGAGACGACC 
ATCACCGACG AAGACGGAGT CTGGGACGAC AAACATCCAG ACAGGGTGAT GGAGGAAGGA 
CAGAAAGAGA CAGAGGAGTC GCACTCAGTG GAACAGACTC CAGAGGACGA GAACCAAACA 
CCTCCAGTGA CCTTCCTGGA GTTTCAGTGG GGTCCGAGCC AAGACCTGCA GCAGGTCTAC 
CAGCAGAAAC GCCAAGCTCT GCTCCAGAGA TCATCGCACA GGGTCGAGGA AATCAAGTCC 
AGAAGAGATG GTCCAAAGGT TCAGCCTGAG GTCCAAGCTC CGCCCAGAGA GCAGTCTAAA 
GCTAAAGACT CTCAACCTGT CAGCTGTAAA TCAAAGACTA AGACTAAGAC TGAACCTCAG 
CCTAAGACGA ACACAAAGTC AGAGGGAGGA CGGGCTGAGC AACAGACGTC TGACAAGTCT 
GGTTTCAAGC AGAAAAAGGT CCAGTGTCGA CCTTCAGTGA GCGACTGCAG GCTGAAGATC 
TGCACACCGG AGCAGAGGAA GCTGGATGTT ACTGAGATGC ATCAGAGGAC TCAGAGACTG 
TACGAGCAGC TGGAGGAGGT GAAGCATCAG AAAGCAGCGA GGAGCCGACA GGAGGCGTCT 
GCAGAGAACA GGCTGAAGGC CAAAGAGTTC CACAAGAAAA CGTTACAGAA ACTTCGTGCC 
AAGACGACTC GGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019110989.1
CDS	293..6010
Translation	MKRKVSQLRLSPNEEARLIREEHERRRKLRIQQVREQQRYIALQIRREVELRRRRELEQLKEELRDDWERQQREKLNTLQKLYQESLQLLGQGHRSAKENEPDLAAIAQREEENHSKAEERYREALNELKSQRLKDRERQSRSINARKKALQAEKERAAKVASLPPPPPNPIQNIDSKKPHVVKKSDVSAFAATHYHMPESTVDREADPTQPNAHEEAELEARRLQDLQREEKQRREEQLEKARLRGKQALRREHLVQDRERLLLELEHMQQTDLLRRRQQVSHMPAQIFQPLYKRQEMREDFQREMEFAFEDMYTGERRVKGDLVVQLVPEPLPALSTGSQDQDQELDITLDEIPTPAAENTHHDTEQEAGSPEQEASARETSRPAPRRALKKLLDRIRSQRTHWSEHNGHVPAADSPTAFTNQIPEHDTTIDTGSLTSEEKDKLPEPARSPPAVSETTEPSAEAETSAPVRLQEFTDERRKRELELEQEKQQQVVLIQELEEQKAKLEQMLLEAQQEREHLKAAVTQEVPVLDQEVTSVSSGLSTELVPPPGEDEHSRRIREYQQRLLEQNRIHQRSVEVARQRLEEYQRALQIRYSMTKQSLPSVMPPGLVHPPLHSTWPVHLPAPPSLPTTPAVVAYVPARPHPSVEVTTREADMVASPPHLPGSSLRVCSRLLPDEVESVWNRAKNQKPDVSTWLTDNIMERVTEHLPDSVKPVSVTMKPPPHKLSTTRESTSILLQPTSHPIQVIGPSIADRTPLFPGHVAVKPHVSLLTGPLSSREDDMERQRQQEEEEEEERQRWELQEVQRRLLEQREAVEVQQRQKEEERQRQELQRRMQEQRAAVELQQKQQEEERQRRLLEQREAMEVQQRQTEEEKQRRELQEVQRRLLEQREAVELQKKQQEEERQRREVEMEEMRRQKETLQALIHTDAQPGAEVSSEVPLSESIGPTRRQLLASLLRAIQETNGGTLSHLEEPQEEDDSPQQPSSSYSVETDPVFQSSVPAGPLPASGLLPPPRAAKPPVTRVRLGVMKMMTEQHELSAIQEVETPVNSSLVTGLEDSVKLPSQTVGWDPQEESDSSVSSDRTLQTPSWSSSEQRPAERREASSRRYSHLDLLMEAGTSPESSESSDSVVRKTSRLSSDSGRGADYSGPAITSYRSFTESAQRPPDSDCLSSTTISSGSYITTDPEQNEPNVNTDKSLPIRRHVEQDGGANTLNVSSQSVQNFSMNSSTAARPAPDVDSLFSDSSIQRIIDRYTRELNFSLSTTGRAADSEGSHVDDPGSVSQQSLVRVSERSQCLPSDAAAAQRSVQEWDNTVDPILEHFSVEGTEQDSFRPLIGRLADQSSCLAVDHRDSTMEQLVGQPSAHSSMIGQLPGLPVGLDQGGWDSTLSRMIGRLSHQSSPHWLSGGHDFYAGQMVSERSATWLDEESRMRPLVGELDESTGHHSGSSGERTRVDPDVSTEASVPSFPALPPEASSHSASDPAVNPHPQDQNQTSPMDLGPERTEDSFHLLVAEVTHNETAEPSVNFHAVPDSPEGRPASCEQSASAPPDEFETHDDPSIESEPSPERLRTDKPPSCLTPPHALHESFSQLITSQHHPHEVELTALSLSNLTMCDDALTLKLQPEEPFRELESEQTVRPASEKLSEAGGEKGILEQSEITLVSLTDTTLQDQETTITDEDGVWDDKHPDRVMEEGQKETEESHSVEQTPEDENQTPPVTFLEFQWGPSQDLQQVYQQKRQALLQRSSHRVEEIKSRRDGPKVQPEVQAPPREQSKAKDSQPVSCKSKTKTKTEPQPKTNTKSEGGRAEQQTSDKSGFKQKKVQCRPSVSDCRLKICTPEQRKLDVTEMHQRTQRLYEQLEEVKHQKAARSRQEASAENRLKAKEFHKKTLQKLRAKTTRQ

Target ORF information:

RefSeq Version	XM_019255444.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea centrosomal protein 295 (cep295), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019255444.1

ORF Insert Sequence:

ATGAAGAGGA AAGTTTCCCA GCTCAGACTG AGCCCCAACG AGGAGGCCAG GCTGATCCGA 
GAGGAGCATG AGAGGAGGAG GAAGCTGCGA ATACAGCAGG TGCGGGAGCA GCAGCGGTAC 
ATCGCGCTGC AGATCCGCCG AGAGGTCGAG TTGAGGCGGC GGCGTGAGCT CGAGCAGCTG 
AAGGAGGAGC TGAGGGACGA CTGGGAGCGA CAGCAGAGGG AGAAACTCAA CACACTGCAG 
AAACTTTACC AGGAGAGCCT CCAGCTTCTC GGCCAAGGAC ACAGGAGCGC AAAAGAAAAC 
GAACCCGACC TGGCAGCCAT AGCTCAGAGG GAGGAGGAAA ATCACAGCAA AGCAGAGGAG 
CGTTATCGAG AAGCTCTGAA TGAGCTCAAA TCACAGAGGC TTAAAGACCG TGAGAGGCAG 
AGCCGTTCCA TCAATGCCAG GAAGAAGGCG CTACAGGCAG AAAAGGAGAG AGCGGCAAAA 
GTGGCCAGCC TCCCGCCGCC GCCGCCAAAC CCCATTCAGA ACATCGATTC AAAGAAGCCT 
CATGTGGTAA AGAAGTCTGA TGTGAGCGCC TTTGCTGCTA CACATTACCA CATGCCTGAG 
AGCACAGTGG ACAGAGAGGC AGACCCAACA CAGCCTAACG CTCACGAGGA AGCTGAGCTG 
GAGGCGAGGA GACTGCAGGA CCTCCAGAGG GAGGAGAAGC AGAGGAGAGA GGAGCAGCTG 
GAGAAGGCTC GTCTCAGAGG GAAGCAGGCT CTGAGGAGGG AACACCTCGT GCAGGACCGT 
GAGCGCTTGC TCTTGGAGCT GGAACACATG CAGCAGACAG ACCTGCTGAG GAGGAGACAG 
CAGGTGTCAC ACATGCCTGC TCAGATCTTC CAGCCTCTCT ACAAGAGACA GGAGATGAGG 
GAGGACTTCC AGAGGGAGAT GGAGTTCGCC TTTGAGGACA TGTACACTGG AGAGAGGAGG 
GTTAAAGGTG ATCTGGTGGT CCAGCTGGTA CCGGAGCCTC TGCCAGCTCT GTCCACAGGC 
AGCCAAGACC AGGACCAGGA GCTGGACATC ACGCTGGACG AAATCCCCAC ACCAGCAGCA 
GAAAACACAC ACCATGACAC TGAGCAAGAA GCTGGAAGCC CTGAGCAGGA AGCATCTGCA 
CGAGAGACCT CCAGACCTGC TCCCAGACGG GCGTTGAAGA AACTCCTGGA TCGTATCAGG 
AGCCAGCGGA CTCACTGGAG CGAACACAAC GGTCACGTCC CCGCAGCCGA CTCGCCGACC 
GCCTTCACCA ATCAGATACC AGAGCACGAC ACAACCATCG ACACGGGATC TCTGACCAGT 
GAGGAGAAGG ACAAACTCCC TGAACCCGCC CGCTCTCCAC CCGCAGTTTC AGAGACAACA 
GAGCCGTCAG CTGAAGCAGA AACTTCGGCT CCTGTCCGAC TCCAAGAATT TACAGACGAA 
CGAAGGAAAA GGGAGTTGGA GCTCGAGCAG GAGAAGCAGC AGCAGGTGGT GTTGATCCAG 
GAGTTAGAGG AGCAGAAGGC CAAACTGGAG CAGATGCTGC TCGAAGCACA GCAGGAGAGA 
GAGCACCTGA AGGCTGCTGT GACCCAGGAA GTTCCTGTCC TCGACCAGGA AGTCACCTCC 
GTCTCCTCCG GGCTGTCCAC TGAGCTGGTG CCTCCTCCGG GTGAAGATGA ACACAGCAGA 
AGGATTCGAG AATACCAACA GAGGCTGCTG GAACAGAACA GAATTCACCA GAGGTCGGTG 
GAGGTGGCTC GTCAGCGGCT GGAGGAATAC CAGCGAGCTC TACAGATTCG TTACAGCATG 
ACCAAACAGT CGCTGCCCTC CGTCATGCCT CCAGGACTCG TTCACCCGCC GCTACACAGT 
ACTTGGCCTG TTCATCTTCC TGCTCCTCCG TCACTCCCGA CAACTCCTGC AGTGGTTGCA 
TACGTCCCTG CCAGACCACA CCCCTCTGTG GAAGTCACTA CAAGAGAGGC TGACATGGTG 
GCTTCACCTC CCCACCTTCC TGGCTCCAGT TTGAGGGTTT GCTCCAGGTT GCTGCCAGAT 
GAAGTGGAAT CTGTTTGGAA CAGAGCGAAG AACCAGAAAC CCGATGTTTC TACCTGGCTG 
ACTGACAACA TAATGGAGAG AGTGACTGAG CACCTTCCAG ACAGCGTGAA ACCCGTCTCA 
GTCACCATGA AGCCACCGCC CCACAAACTG TCCACAACAC GTGAGTCTAC CAGCATCCTG 
CTTCAGCCGA CCTCTCATCC CATCCAAGTC ATCGGCCCCA GCATTGCAGA TAGAACACCT 
CTGTTTCCAG GCCATGTAGC GGTTAAGCCG CATGTCTCCC TGCTGACTGG GCCCCTGAGC 
TCCAGAGAGG ACGACATGGA GAGGCAGAGG CAACAAGAGG AGGAGGAGGA GGAGGAGAGG 
CAGAGATGGG AGCTGCAGGA GGTTCAGAGA CGATTGCTGG AGCAGAGGGA GGCAGTGGAA 
GTGCAACAGA GGCAGAAAGA AGAGGAGAGG CAGAGACAGG AGCTGCAGAG ACGAATGCAG 
GAGCAAAGAG CGGCAGTAGA GCTCCAGCAG AAGCAGCAAG AAGAGGAGAG GCAGAGACGG 
TTGCTGGAGC AGAGGGAGGC GATGGAGGTG CAACAAAGGC AGACAGAGGA GGAGAAGCAG 
AGGCGGGAGC TGCAGGAGGT TCAGAGACGG TTGTTGGAGC AGAGGGAGGC AGTGGAGCTG 
CAGAAGAAGC AACAAGAAGA GGAGAGGCAG AGGCGGGAAG TGGAGATGGA GGAGATGAGG 
CGACAGAAGG AGACGTTACA GGCTCTGATT CATACTGACG CACAGCCAGG TGCAGAGGTC 
TCCAGTGAAG TGCCGCTTTC AGAGAGCATC GGTCCAACTC GACGTCAGTT ACTTGCATCA 
CTGCTGCGAG CCATACAGGA AACTAACGGA GGAACTCTAT CACATCTAGA AGAGCCGCAG 
GAGGAAGATG ACTCTCCTCA ACAACCATCA TCATCTTACA GCGTCGAGAC TGACCCCGTC 
TTTCAGAGCA GTGTTCCTGC TGGACCTCTC CCAGCATCAG GCCTCCTGCC TCCTCCTCGA 
GCAGCGAAGC CTCCAGTGAC TCGCGTCAGG CTGGGCGTCA TGAAGATGAT GACTGAACAA 
CATGAGCTCA GTGCTATTCA AGAGGTGGAG ACGCCAGTCA ACAGCAGCCT GGTCACAGGT 
CTGGAGGACA GTGTGAAGCT TCCTTCACAG ACTGTGGGCT GGGATCCACA GGAGGAGTCC 
GACTCGTCGG TGTCCTCTGA CAGGACTCTG CAGACGCCCT CTTGGTCCAG CAGCGAGCAG 
CGGCCTGCTG AAAGACGAGA GGCGAGCTCG AGGAGGTACA GCCATCTGGA TCTGCTGATG 
GAGGCTGGAA CATCTCCAGA ATCCTCAGAG TCATCTGATT CAGTCGTGAG GAAAACGTCA 
CGGCTTTCCT CTGACTCTGG CAGAGGAGCT GACTACTCCG GACCTGCGAT CACGAGCTAC 
AGATCCTTCA CAGAGTCTGC ACAGAGGCCT CCTGACTCTG ACTGCCTCTC CTCCACCACC 
ATCTCCAGCG GCAGCTACAT CACCACAGAC CCTGAACAAA ACGAGCCAAA CGTAAACACT 
GATAAATCTC TGCCCATCAG ACGTCATGTG GAACAAGATG GAGGAGCAAA TACGCTGAAC 
GTCTCCTCTC AGTCGGTTCA AAACTTCTCC ATGAACAGCT CGACTGCAGC CCGTCCTGCT 
CCGGATGTCG ACTCTCTGTT TAGTGACAGC AGCATCCAGC GAATCATCGA CCGATACACG 
AGAGAGCTCA ACTTCTCCCT CAGCACCACC GGGAGAGCCG CAGACAGTGA AGGCTCTCAT 
GTGGACGATC CCGGCTCAGT CTCTCAGCAG TCTTTGGTTC GAGTCTCAGA GAGGAGTCAG 
TGTCTTCCCT CAGACGCAGC AGCAGCACAG AGGAGTGTCC AGGAATGGGA CAACACAGTC 
GATCCAATCC TGGAGCACTT CTCAGTTGAG GGCACAGAGC AGGACTCCTT CAGACCTCTG 
ATTGGCCGGC TGGCAGACCA ATCATCCTGC TTGGCTGTCG ACCACAGGGA CTCGACCATG 
GAGCAGCTGG TCGGTCAACC ATCGGCTCAC TCGTCCATGA TCGGTCAGCT GCCCGGGCTG 
CCGGTGGGCC TCGATCAAGG CGGATGGGAT TCCACTCTGA GCCGGATGAT TGGTCGACTC 
TCCCATCAGT CCAGCCCTCA TTGGCTGAGT GGAGGCCACG ACTTCTATGC GGGTCAGATG 
GTGTCAGAGC GATCAGCCAC GTGGTTGGAT GAAGAGAGCC GGATGAGACC GCTGGTCGGA 
GAGCTGGATG AGTCCACTGG ACACCACAGT GGGAGCTCAG GTGAAAGAAC TCGCGTGGAT 
CCCGATGTTT CCACTGAGGC CAGTGTGCCG TCATTTCCAG CGTTGCCTCC TGAAGCGTCG 
TCACACAGCG CCTCTGACCC GGCTGTAAAT CCACACCCAC AGGACCAGAA CCAAACCAGT 
CCAATGGACC TGGGCCCAGA GCGGACTGAA GACTCGTTCC ACCTGCTCGT GGCTGAAGTC 
ACCCACAACG AAACAGCCGA GCCCTCCGTC AACTTCCACG CTGTGCCTGA CTCTCCTGAA 
GGTCGGCCAG CCAGCTGTGA ACAGAGCGCC TCTGCACCGC CTGATGAGTT TGAAACCCAC 
GACGACCCGT CCATCGAGTC TGAACCGTCT CCTGAGCGCC TCAGGACAGA CAAGCCGCCC 
AGCTGCCTGA CGCCGCCCCA CGCCTTACAT GAGTCTTTCT CCCAGCTCAT CACCTCCCAG 
CACCACCCCC ATGAGGTGGA GCTAACCGCT CTGAGTCTGT CAAATCTCAC CATGTGTGAT 
GATGCACTGA CTCTGAAGCT GCAGCCTGAG GAACCGTTCA GAGAGCTGGA GTCTGAGCAG 
ACTGTTCGTC CTGCCTCAGA GAAGCTGTCG GAGGCTGGCG GTGAGAAAGG CATCCTGGAG 
CAGTCGGAGA TAACACTCGT GAGCCTGACA GACACAACTC TGCAGGACCA AGAGACGACC 
ATCACCGACG AAGACGGAGT CTGGGACGAC AAACATCCAG ACAGGGTGAT GGAGGAAGGA 
CAGAAAGAGA CAGAGGAGTC GCACTCAGTG GAACAGACTC CAGAGGACGA GAACCAAACA 
CCTCCAGTGA CCTTCCTGGA GTTTCAGTGG GGTCCGAGCC AAGACCTGCA GCAGGTCTAC 
CAGCAGAAAC GCCAAGCTCT GCTCCAGAGA TCATCGCACA GGGTCGAGGA AATCAAGTCC 
AGAAGAGATG GTCCAAAGGT TCAGCCTGAG GTCCAAGCTC CGCCCAGAGA GCAGTCTAAA 
GCTAAAGACT CTCAACCTGT CAGCTGTAAA TCAAAGACTA AGACTAAGAC TGAACCTCAG 
CCTAAGACGA ACACAAAGTC AGAGGGAGGA CGGGCTGAGC AACAGACGTC TGACAAGTCT 
GGTTTCAAGC AGAAAAAGGT CCAGTGTCGA CCTTCAGTGA GCGACTGCAG GCTGAAGATC 
TGCACACCGG AGCAGAGGAA GCTGGATGTT ACTGAGATGC ATCAGAGGAC TCAGAGACTG 
TACGAGCAGC TGGAGGAGGT GAAGCATCAG AAAGCAGCGA GGAGCCGACA GGAGGCGTCT 
GCAGAGAACA GGCTGAAGGC CAAAGAGTTC CACAAGAAAA CGTTACAGAA ACTTCGTGCC 
AAGACGACTC GGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

cep295 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム