pof1b cDNA ORF clone, Labrus bergylta(ballan wrasse)

Gene
Clones
gRNAs

Summary

Gene Symbol	pof1b
Entrez Gene ID	110002937
Full Name	POF1B, actin binding protein
Gene Type	protein-coding
Organism	Labrus bergylta(ballan wrasse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020658622.1	XP_020514278.1	protein POF1B
XM_020658622.2	XP_020514278.1	protein POF1B

Pathways from BioSystems
Gene Ontology

The following pof1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pof1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa230097	XM_020658622.2 Latest version!	Labrus bergylta POF1B actin binding protein (pof1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OLa146730	XM_020658622.1 Latest version!	Labrus bergylta premature ovarian failure, 1B (pof1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pof1b ( XM_020658622.2 ) cDNA ORF clone, Labrus bergylta(ballan wrasse) -> XP_020514278.1 Labrus bergylta POF1B actin binding protein (pof1b), mRNA.

Price & Availability↑

CloneID	OLa230097
Clone ID Related Accession (Same CDS sequence)	XM_020658622.2
Accession Version	XM_020658622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-22
Organism	Labrus bergylta(ballan wrasse)
Product	protein POF1B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018115494.1) and transcript sequence (GFFM01026165.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 23, 2019 this sequence version replaced XM_020658622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Labrus bergylta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 701 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCTGTTA ATTCGCGCCA CAAGACGACT TCTACGACCT ACCGCACCGT CAAAGTGGCC 
TCTCCGGAGC CGGTGACCAG CACGGTGACC AGCACGGTGA TGTCCGCCGG GTCGGTGTCC 
CCGTTGCCGC ACGCGCTGAA CGGTAACTAC GAGACGGTGA GGTACCTGGT CCCGGTGCAG 
CAGGCCATGC AGCAGCAGCA GCAGTCCTTC GTGCTCATGC AGCAGCCCAT GATGCAGCAG 
CATATTATCC AGCAGCCCAT GATGCAGCAG CCCATGATCC AGCAGCCCAT GATGCAGCAG 
ATGGTCTCTC CGGTGTACCT GCAGGGTATG ACGCACCTGA GCGTCAGCAG CCAGGACACC 
GACATGTACC TGCAGCGCAG CACCCTGGAG AGCGTCAGCG GACAGTCGTC AGTGTTCAGC 
CAGTCGTCCA GGAGCCCTGA GCCCGAGGAG GTGGAGGAAG AGGAGGAGGA GGAGGAAGTG 
GAGGAGGAAG AAGAGGAGGT CGTGGAAGTG GAAGAGACGG AGATCGTCAA CATCGTGCAG 
TCGAAGCCCC CGCCGCCGAT CGAGAGGATC TCCAGGACCG AGGTGAGGAC CGAGATGAAG 
GAGATCCCTC AGCCGACCAA GATGGACACG CGGTACTTCG GCGAGCTGCT GGCCGACGTC 
TACAGGAAGA ACTGCGACAT CCACTCCTGC ATCTTCGAGC ACGTGTCCAA GATCAGAGGA 
CAGAAACACC TTCTGGATCC CAGCAATGAC TACAAGGTGG AGAAAGAGGA GGTGGAGTCT 
TTGCTTCCCA AAGGAGCCAC TGAACTGACC AAACAACAGA TCCGCTACCT GCTGCAGACT 
CGTCTGACTG CAGATAAAAG CATGCGTCTG CTGCTGTCCA CCTTCAGCAG CCTGAGAGAG 
GAGCTGCTGC ACATGTCTGA GGACCTGAGG CGTCTGGAGA GCGAGAAGGA GGCTCTGGAG 
CGAGATCTGA GCTTCAAGGC GGATCAGGCT CGTCAGTATG ACTGTCTCCT GGAGGCTGTG 
CGAGAAAACA ACAGACAGCT GCAGATGTCT CTTAAAGAAA GCAGCTCTGC TCAGCGCTCT 
CTGGAGACTC AGCTCATGAG CAGCAGAAGC ACCGACTCCA GCAGAGACTT CAAAGTGAAA 
GAACAGGACG GGAGGCTGAG AGCGCTGGAG AAGGAGAACG AGATGCTGAG ACAGAAGCTG 
TCAGGTCAAG GCAGCAGCAG CACGCTGCAC ATCAAGACGG AGGAGCTGTC CCGTCAGTAC 
AACGAGCAGC TGGACAGCAT GAGGAAAGAG AAGGACCAGG AGATCCAGAG ACTGAGGACC 
CAGATAACAA GGATCCAGAC CGAGACCACC ACAACCACCT CGTCCGACAA CAGTCTGCAG 
CTGAAGATCT CAGAGCTGCT CGCCATGTTG CAGCAGAGAC AGACCACCAT CACCCGACAG 
GAAGAGGAGA TCAGGAAGCT GATGCAGGAA AGGAGTGACG GCTCCAAGAA CGTCACCAAG 
ACCGTCATCA CCAAGAGGTA CAGGAACCAG TACCCGTTGC TGGGTCTGCT GAGCGACGAC 
TACAGTACCA CCTCGCAAGT CAGAGAAGAC AGCACCATCT TAGTCGAGAG AAGCGGAGAG 
ATCATCAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020514278.1
CDS	220..1854
Translation	MSVNSRHKTTSTTYRTVKVASPEPVTSTVTSTVMSAGSVSPLPHALNGNYETVRYLVPVQQAMQQQQQSFVLMQQPMMQQHIIQQPMMQQPMIQQPMMQQMVSPVYLQGMTHLSVSSQDTDMYLQRSTLESVSGQSSVFSQSSRSPEPEEVEEEEEEEEVEEEEEEVVEVEETEIVNIVQSKPPPPIERISRTEVRTEMKEIPQPTKMDTRYFGELLADVYRKNCDIHSCIFEHVSKIRGQKHLLDPSNDYKVEKEEVESLLPKGATELTKQQIRYLLQTRLTADKSMRLLLSTFSSLREELLHMSEDLRRLESEKEALERDLSFKADQARQYDCLLEAVRENNRQLQMSLKESSSAQRSLETQLMSSRSTDSSRDFKVKEQDGRLRALEKENEMLRQKLSGQGSSSTLHIKTEELSRQYNEQLDSMRKEKDQEIQRLRTQITRIQTETTTTTSSDNSLQLKISELLAMLQQRQTTITRQEEEIRKLMQERSDGSKNVTKTVITKRYRNQYPLLGLLSDDYSTTSQVREDSTILVERSGEIIKK

Target ORF information:

RefSeq Version	XM_020658622.2
Organism	Labrus bergylta(ballan wrasse)
Definition	Labrus bergylta POF1B actin binding protein (pof1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020658622.2

ORF Insert Sequence:

ATGTCTGTTA ATTCGCGCCA CAAGACGACT TCTACGACCT ACCGCACCGT CAAAGTGGCC 
TCTCCGGAGC CGGTGACCAG CACGGTGACC AGCACGGTGA TGTCCGCCGG GTCGGTGTCC 
CCGTTGCCGC ACGCGCTGAA CGGTAACTAC GAGACGGTGA GGTACCTGGT CCCGGTGCAG 
CAGGCCATGC AGCAGCAGCA GCAGTCCTTC GTGCTCATGC AGCAGCCCAT GATGCAGCAG 
CATATTATCC AGCAGCCCAT GATGCAGCAG CCCATGATCC AGCAGCCCAT GATGCAGCAG 
ATGGTCTCTC CGGTGTACCT GCAGGGTATG ACGCACCTGA GCGTCAGCAG CCAGGACACC 
GACATGTACC TGCAGCGCAG CACCCTGGAG AGCGTCAGCG GACAGTCGTC AGTGTTCAGC 
CAGTCGTCCA GGAGCCCTGA GCCCGAGGAG GTGGAGGAAG AGGAGGAGGA GGAGGAAGTG 
GAGGAGGAAG AAGAGGAGGT CGTGGAAGTG GAAGAGACGG AGATCGTCAA CATCGTGCAG 
TCGAAGCCCC CGCCGCCGAT CGAGAGGATC TCCAGGACCG AGGTGAGGAC CGAGATGAAG 
GAGATCCCTC AGCCGACCAA GATGGACACG CGGTACTTCG GCGAGCTGCT GGCCGACGTC 
TACAGGAAGA ACTGCGACAT CCACTCCTGC ATCTTCGAGC ACGTGTCCAA GATCAGAGGA 
CAGAAACACC TTCTGGATCC CAGCAATGAC TACAAGGTGG AGAAAGAGGA GGTGGAGTCT 
TTGCTTCCCA AAGGAGCCAC TGAACTGACC AAACAACAGA TCCGCTACCT GCTGCAGACT 
CGTCTGACTG CAGATAAAAG CATGCGTCTG CTGCTGTCCA CCTTCAGCAG CCTGAGAGAG 
GAGCTGCTGC ACATGTCTGA GGACCTGAGG CGTCTGGAGA GCGAGAAGGA GGCTCTGGAG 
CGAGATCTGA GCTTCAAGGC GGATCAGGCT CGTCAGTATG ACTGTCTCCT GGAGGCTGTG 
CGAGAAAACA ACAGACAGCT GCAGATGTCT CTTAAAGAAA GCAGCTCTGC TCAGCGCTCT 
CTGGAGACTC AGCTCATGAG CAGCAGAAGC ACCGACTCCA GCAGAGACTT CAAAGTGAAA 
GAACAGGACG GGAGGCTGAG AGCGCTGGAG AAGGAGAACG AGATGCTGAG ACAGAAGCTG 
TCAGGTCAAG GCAGCAGCAG CACGCTGCAC ATCAAGACGG AGGAGCTGTC CCGTCAGTAC 
AACGAGCAGC TGGACAGCAT GAGGAAAGAG AAGGACCAGG AGATCCAGAG ACTGAGGACC 
CAGATAACAA GGATCCAGAC CGAGACCACC ACAACCACCT CGTCCGACAA CAGTCTGCAG 
CTGAAGATCT CAGAGCTGCT CGCCATGTTG CAGCAGAGAC AGACCACCAT CACCCGACAG 
GAAGAGGAGA TCAGGAAGCT GATGCAGGAA AGGAGTGACG GCTCCAAGAA CGTCACCAAG 
ACCGTCATCA CCAAGAGGTA CAGGAACCAG TACCCGTTGC TGGGTCTGCT GAGCGACGAC 
TACAGTACCA CCTCGCAAGT CAGAGAAGAC AGCACCATCT TAGTCGAGAG AAGCGGAGAG 
ATCATCAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pof1b ( XM_020658622.1 ) cDNA ORF clone, Labrus bergylta(ballan wrasse) -> XP_020514278.1 Labrus bergylta premature ovarian failure, 1B (pof1b), mRNA.

Price & Availability↑

CloneID	OLa146730
Clone ID Related Accession (Same CDS sequence)	XM_020658622.1
Accession Version	XM_020658622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-26
Organism	Labrus bergylta(ballan wrasse)
Product	protein POF1B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018115494.1) and transcript sequence (GFDP01009250.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Labrus bergylta Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 257 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCTGTTA ATTCGCGCCA CAAGACGACT TCTACGACCT ACCGCACCGT CAAAGTGGCC 
TCTCCGGAGC CGGTGACCAG CACGGTGACC AGCACGGTGA TGTCCGCCGG GTCGGTGTCC 
CCGTTGCCGC ACGCGCTGAA CGGTAACTAC GAGACGGTGA GGTACCTGGT CCCGGTGCAG 
CAGGCCATGC AGCAGCAGCA GCAGTCCTTC GTGCTCATGC AGCAGCCCAT GATGCAGCAG 
CATATTATCC AGCAGCCCAT GATGCAGCAG CCCATGATCC AGCAGCCCAT GATGCAGCAG 
ATGGTCTCTC CGGTGTACCT GCAGGGTATG ACGCACCTGA GCGTCAGCAG CCAGGACACC 
GACATGTACC TGCAGCGCAG CACCCTGGAG AGCGTCAGCG GACAGTCGTC AGTGTTCAGC 
CAGTCGTCCA GGAGCCCTGA GCCCGAGGAG GTGGAGGAAG AGGAGGAGGA GGAGGAAGTG 
GAGGAGGAAG AAGAGGAGGT CGTGGAAGTG GAAGAGACGG AGATCGTCAA CATCGTGCAG 
TCGAAGCCCC CGCCGCCGAT CGAGAGGATC TCCAGGACCG AGGTGAGGAC CGAGATGAAG 
GAGATCCCTC AGCCGACCAA GATGGACACG CGGTACTTCG GCGAGCTGCT GGCCGACGTC 
TACAGGAAGA ACTGCGACAT CCACTCCTGC ATCTTCGAGC ACGTGTCCAA GATCAGAGGA 
CAGAAACACC TTCTGGATCC CAGCAATGAC TACAAGGTGG AGAAAGAGGA GGTGGAGTCT 
TTGCTTCCCA AAGGAGCCAC TGAACTGACC AAACAACAGA TCCGCTACCT GCTGCAGACT 
CGTCTGACTG CAGATAAAAG CATGCGTCTG CTGCTGTCCA CCTTCAGCAG CCTGAGAGAG 
GAGCTGCTGC ACATGTCTGA GGACCTGAGG CGTCTGGAGA GCGAGAAGGA GGCTCTGGAG 
CGAGATCTGA GCTTCAAGGC GGATCAGGCT CGTCAGTATG ACTGTCTCCT GGAGGCTGTG 
CGAGAAAACA ACAGACAGCT GCAGATGTCT CTTAAAGAAA GCAGCTCTGC TCAGCGCTCT 
CTGGAGACTC AGCTCATGAG CAGCAGAAGC ACCGACTCCA GCAGAGACTT CAAAGTGAAA 
GAACAGGACG GGAGGCTGAG AGCGCTGGAG AAGGAGAACG AGATGCTGAG ACAGAAGCTG 
TCAGGTCAAG GCAGCAGCAG CACGCTGCAC ATCAAGACGG AGGAGCTGTC CCGTCAGTAC 
AACGAGCAGC TGGACAGCAT GAGGAAAGAG AAGGACCAGG AGATCCAGAG ACTGAGGACC 
CAGATAACAA GGATCCAGAC CGAGACCACC ACAACCACCT CGTCCGACAA CAGTCTGCAG 
CTGAAGATCT CAGAGCTGCT CGCCATGTTG CAGCAGAGAC AGACCACCAT CACCCGACAG 
GAAGAGGAGA TCAGGAAGCT GATGCAGGAA AGGAGTGACG GCTCCAAGAA CGTCACAAAG 
ACCGTCATCA CCAAGAGGTA CAGGAACCAG TACCCGTTGC TGGGTCTGCT GAGCGACGAC 
TACAGTACCA CCTCGCAAGT CAGAGAAGAC AGCACCATCT TAGTCGAGAG AAGCGGAGAG 
ATCATCAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020514278.1
CDS	226..1860
Translation	MSVNSRHKTTSTTYRTVKVASPEPVTSTVTSTVMSAGSVSPLPHALNGNYETVRYLVPVQQAMQQQQQSFVLMQQPMMQQHIIQQPMMQQPMIQQPMMQQMVSPVYLQGMTHLSVSSQDTDMYLQRSTLESVSGQSSVFSQSSRSPEPEEVEEEEEEEEVEEEEEEVVEVEETEIVNIVQSKPPPPIERISRTEVRTEMKEIPQPTKMDTRYFGELLADVYRKNCDIHSCIFEHVSKIRGQKHLLDPSNDYKVEKEEVESLLPKGATELTKQQIRYLLQTRLTADKSMRLLLSTFSSLREELLHMSEDLRRLESEKEALERDLSFKADQARQYDCLLEAVRENNRQLQMSLKESSSAQRSLETQLMSSRSTDSSRDFKVKEQDGRLRALEKENEMLRQKLSGQGSSSTLHIKTEELSRQYNEQLDSMRKEKDQEIQRLRTQITRIQTETTTTTSSDNSLQLKISELLAMLQQRQTTITRQEEEIRKLMQERSDGSKNVTKTVITKRYRNQYPLLGLLSDDYSTTSQVREDSTILVERSGEIIKK

Target ORF information:

RefSeq Version	XM_020658622.1
Organism	Labrus bergylta(ballan wrasse)
Definition	Labrus bergylta premature ovarian failure, 1B (pof1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020658622.1

ORF Insert Sequence:

ATGTCTGTTA ATTCGCGCCA CAAGACGACT TCTACGACCT ACCGCACCGT CAAAGTGGCC 
TCTCCGGAGC CGGTGACCAG CACGGTGACC AGCACGGTGA TGTCCGCCGG GTCGGTGTCC 
CCGTTGCCGC ACGCGCTGAA CGGTAACTAC GAGACGGTGA GGTACCTGGT CCCGGTGCAG 
CAGGCCATGC AGCAGCAGCA GCAGTCCTTC GTGCTCATGC AGCAGCCCAT GATGCAGCAG 
CATATTATCC AGCAGCCCAT GATGCAGCAG CCCATGATCC AGCAGCCCAT GATGCAGCAG 
ATGGTCTCTC CGGTGTACCT GCAGGGTATG ACGCACCTGA GCGTCAGCAG CCAGGACACC 
GACATGTACC TGCAGCGCAG CACCCTGGAG AGCGTCAGCG GACAGTCGTC AGTGTTCAGC 
CAGTCGTCCA GGAGCCCTGA GCCCGAGGAG GTGGAGGAAG AGGAGGAGGA GGAGGAAGTG 
GAGGAGGAAG AAGAGGAGGT CGTGGAAGTG GAAGAGACGG AGATCGTCAA CATCGTGCAG 
TCGAAGCCCC CGCCGCCGAT CGAGAGGATC TCCAGGACCG AGGTGAGGAC CGAGATGAAG 
GAGATCCCTC AGCCGACCAA GATGGACACG CGGTACTTCG GCGAGCTGCT GGCCGACGTC 
TACAGGAAGA ACTGCGACAT CCACTCCTGC ATCTTCGAGC ACGTGTCCAA GATCAGAGGA 
CAGAAACACC TTCTGGATCC CAGCAATGAC TACAAGGTGG AGAAAGAGGA GGTGGAGTCT 
TTGCTTCCCA AAGGAGCCAC TGAACTGACC AAACAACAGA TCCGCTACCT GCTGCAGACT 
CGTCTGACTG CAGATAAAAG CATGCGTCTG CTGCTGTCCA CCTTCAGCAG CCTGAGAGAG 
GAGCTGCTGC ACATGTCTGA GGACCTGAGG CGTCTGGAGA GCGAGAAGGA GGCTCTGGAG 
CGAGATCTGA GCTTCAAGGC GGATCAGGCT CGTCAGTATG ACTGTCTCCT GGAGGCTGTG 
CGAGAAAACA ACAGACAGCT GCAGATGTCT CTTAAAGAAA GCAGCTCTGC TCAGCGCTCT 
CTGGAGACTC AGCTCATGAG CAGCAGAAGC ACCGACTCCA GCAGAGACTT CAAAGTGAAA 
GAACAGGACG GGAGGCTGAG AGCGCTGGAG AAGGAGAACG AGATGCTGAG ACAGAAGCTG 
TCAGGTCAAG GCAGCAGCAG CACGCTGCAC ATCAAGACGG AGGAGCTGTC CCGTCAGTAC 
AACGAGCAGC TGGACAGCAT GAGGAAAGAG AAGGACCAGG AGATCCAGAG ACTGAGGACC 
CAGATAACAA GGATCCAGAC CGAGACCACC ACAACCACCT CGTCCGACAA CAGTCTGCAG 
CTGAAGATCT CAGAGCTGCT CGCCATGTTG CAGCAGAGAC AGACCACCAT CACCCGACAG 
GAAGAGGAGA TCAGGAAGCT GATGCAGGAA AGGAGTGACG GCTCCAAGAA CGTCACAAAG 
ACCGTCATCA CCAAGAGGTA CAGGAACCAG TACCCGTTGC TGGGTCTGCT GAGCGACGAC 
TACAGTACCA CCTCGCAAGT CAGAGAAGAC AGCACCATCT TAGTCGAGAG AAGCGGAGAG 
ATCATCAAGA AATGA