LOC105649917 cDNA ORF clone, Jatropha curcas

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105649917
Entrez Gene ID	105649917
Full Name	ribosomal RNA large subunit methyltransferase I
Gene Type	protein-coding
Organism	Jatropha curcas

mRNA and Protein(s)

mRNA	Protein	Name
XM_012236752.2	XP_012092142.1	uncharacterized protein LOC105649917

Pathways from BioSystems
Gene Ontology

The following LOC105649917 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105649917 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OJa42390	XM_012236752.2 Latest version!	Jatropha curcas ribosomal RNA large subunit methyltransferase I (LOC105649917), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105649917 ( XM_012236752.2 ) cDNA ORF clone, Jatropha curcas -> XP_012092142.1 Jatropha curcas ribosomal RNA large subunit methyltransferase I (LOC105649917), mRNA.

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CloneID	OJa42390
Clone ID Related Accession (Same CDS sequence)	XM_012236752.2
Accession Version	XM_012236752.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-05
Organism	Jatropha curcas
Product	uncharacterized protein LOC105649917
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012124099.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 6, 2017 this sequence version replaced XM_012236752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Jatropha curcas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCAGC AGCTGAAGCT TCCGTCCCGC CTCTCAAAGT CTCTGCCTCT TTGTTCATTG 
TCATCTACCC TTGAAGATAT TGCCTCTTCT CACCCAACTA AAGGCATTGC AAAGGTTGTA 
CTGAAAAAAG GGAAGACACA ACTTTTTAAG GATGGAAGCC CAATGGTGTA CAGTGGAGCA 
ATCGATAGAA TAATAGGTAG GCCTCCACCT AAGACTGGAG ATGTTGTGCT TGTTGCTGAT 
GGCACAGAGA AACCTATTGC TTGGGGCTTG TATAATTCTG TCTCCATGTT CAGTGTTAGG 
CTTATGCAAT TAGAAGAGGA AGCGATGGAA GATCCTTCTT GCGCATTGAA CATGGAGAAA 
TTACTTGAAA CAAGAATTAA CGCAGCCATT CAGTTACGCA GGAGGTTGAG TCTTCCTTCA 
GCCCAGACAA ATGCATATCG CCTTCTCAAT AGTGAAGGAG ATAGACTGTC TGGATTAATT 
GTTGACGTCT TTGGAGATTT AGCTGTAATA GCATCATCCG CTGCATGGGT TGAGAAGTAT 
AAACCAGAAG TAGAGGCTTG CATTGGTAGA ATTGATGAAA TTAATCATGT AATCTGGAGA 
CCATCAGCTG AAATTTTAAA AGAAGAAGGA ATGGATGTGT TGGATCTGAA AGAAATGCAT 
TCATCCATTT GTCAAGAAAG GACAAAGGTT GTAGAAAATG GGATCTATTA TGCAATTTCA 
GTTGCAGGGC AAAAGACTGG ATTTTATGCT GATCAACGCG AAAGCCGACA ATTTATATCA 
AACATTTCAG CTGGTCAGAA AGTTCTTGAT ATTTGTTGCT ACAGTGGTGG TTTTGCTCTA 
AATGCAATCC GTGGAGGTGC CTTAGATGTT ATAGGGATCG ATACATCGTT GCCTGCTCTA 
GAGCTTGCTA GAGAAAATAT TATTCTTAAC CATCTGGACC CAAAAAGAAT ATCATTTTTG 
AGAGAAGATG CAACTGAATT TATGAAAGGT GCCCTTTCCA GAAATGAATC ATGGGATGTA 
GTTATTCTGG ATCCTCCTAA ACTGGCACCA AGAAAAAAGG TTCTACAAAG TGCATTGGGC 
ATGTACAGAA ATCTTAACTC ACTGGCAATG AGATTGACGA AGAAAGGTGG TCTTCTCATG 
ACGTGTTCAT GTTCAGGAGC CATGACCCAA AGTGGAATGT TCCTGCGTAT TCTCCAGGGT 
GCTGCATCAA TGGCGGGAAG GAAAATCACA GTCGTACGGC AGGCCGGTGC AGCTTCTGAC 
CATCCTATTG ACCCATCCTA TCTAGAAGGT GCATACCTTA CTAATATCTT GCTTAGAGTG 
TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012092142.1
CDS	100..1425
Translation	MLQQLKLPSRLSKSLPLCSLSSTLEDIASSHPTKGIAKVVLKKGKTQLFKDGSPMVYSGAIDRIIGRPPPKTGDVVLVADGTEKPIAWGLYNSVSMFSVRLMQLEEEAMEDPSCALNMEKLLETRINAAIQLRRRLSLPSAQTNAYRLLNSEGDRLSGLIVDVFGDLAVIASSAAWVEKYKPEVEACIGRIDEINHVIWRPSAEILKEEGMDVLDLKEMHSSICQERTKVVENGIYYAISVAGQKTGFYADQRESRQFISNISAGQKVLDICCYSGGFALNAIRGGALDVIGIDTSLPALELARENIILNHLDPKRISFLREDATEFMKGALSRNESWDVVILDPPKLAPRKKVLQSALGMYRNLNSLAMRLTKKGGLLMTCSCSGAMTQSGMFLRILQGAASMAGRKITVVRQAGAASDHPIDPSYLEGAYLTNILLRVL

Target ORF information:

RefSeq Version	XM_012236752.2
Organism	Jatropha curcas
Definition	Jatropha curcas ribosomal RNA large subunit methyltransferase I (LOC105649917), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012236752.2

ORF Insert Sequence:

ATGCTTCAGC AGCTGAAGCT TCCGTCCCGC CTCTCAAAGT CTCTGCCTCT TTGTTCATTG 
TCATCTACCC TTGAAGATAT TGCCTCTTCT CACCCAACTA AAGGCATTGC AAAGGTTGTA 
CTGAAAAAAG GGAAGACACA ACTTTTTAAG GATGGAAGCC CAATGGTGTA CAGTGGAGCA 
ATCGATAGAA TAATAGGTAG GCCTCCACCT AAGACTGGAG ATGTTGTGCT TGTTGCTGAT 
GGCACAGAGA AACCTATTGC TTGGGGCTTG TATAATTCTG TCTCCATGTT CAGTGTTAGG 
CTTATGCAAT TAGAAGAGGA AGCGATGGAA GATCCTTCTT GCGCATTGAA CATGGAGAAA 
TTACTTGAAA CAAGAATTAA CGCAGCCATT CAGTTACGCA GGAGGTTGAG TCTTCCTTCA 
GCCCAGACAA ATGCATATCG CCTTCTCAAT AGTGAAGGAG ATAGACTGTC TGGATTAATT 
GTTGACGTCT TTGGAGATTT AGCTGTAATA GCATCATCCG CTGCATGGGT TGAGAAGTAT 
AAACCAGAAG TAGAGGCTTG CATTGGTAGA ATTGATGAAA TTAATCATGT AATCTGGAGA 
CCATCAGCTG AAATTTTAAA AGAAGAAGGA ATGGATGTGT TGGATCTGAA AGAAATGCAT 
TCATCCATTT GTCAAGAAAG GACAAAGGTT GTAGAAAATG GGATCTATTA TGCAATTTCA 
GTTGCAGGGC AAAAGACTGG ATTTTATGCT GATCAACGCG AAAGCCGACA ATTTATATCA 
AACATTTCAG CTGGTCAGAA AGTTCTTGAT ATTTGTTGCT ACAGTGGTGG TTTTGCTCTA 
AATGCAATCC GTGGAGGTGC CTTAGATGTT ATAGGGATCG ATACATCGTT GCCTGCTCTA 
GAGCTTGCTA GAGAAAATAT TATTCTTAAC CATCTGGACC CAAAAAGAAT ATCATTTTTG 
AGAGAAGATG CAACTGAATT TATGAAAGGT GCCCTTTCCA GAAATGAATC ATGGGATGTA 
GTTATTCTGG ATCCTCCTAA ACTGGCACCA AGAAAAAAGG TTCTACAAAG TGCATTGGGC 
ATGTACAGAA ATCTTAACTC ACTGGCAATG AGATTGACGA AGAAAGGTGG TCTTCTCATG 
ACGTGTTCAT GTTCAGGAGC CATGACCCAA AGTGGAATGT TCCTGCGTAT TCTCCAGGGT 
GCTGCATCAA TGGCGGGAAG GAAAATCACA GTCGTACGGC AGGCCGGTGC AGCTTCTGAC 
CATCCTATTG ACCCATCCTA TCTAGAAGGT GCATACCTTA CTAATATCTT GCTTAGAGTG 
TTATAA