LOC105649477 cDNA ORF clone, Jatropha curcas

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105649477
Entrez Gene ID	105649477
Full Name	beta-galactosidase 8
Gene Type	protein-coding
Organism	Jatropha curcas

mRNA and Protein(s)

mRNA	Protein	Name
XM_020685589.1	XP_020541248.1	beta-galactosidase 8 isoform X1
XM_012236128.2	XP_012091518.1	beta-galactosidase 8 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC105649477 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105649477 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OJa66113	XM_020685589.1 Latest version!	Jatropha curcas beta-galactosidase 8 (LOC105649477), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OJa66114	XM_012236128.2 Latest version!	Jatropha curcas beta-galactosidase 8 (LOC105649477), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105649477 ( XM_020685589.1 ) cDNA ORF clone, Jatropha curcas -> XP_020541248.1 Jatropha curcas beta-galactosidase 8 (LOC105649477), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa66113
Clone ID Related Accession (Same CDS sequence)	XM_020685589.1
Accession Version	XM_020685589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-05
Organism	Jatropha curcas
Product	beta-galactosidase 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012125517.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Jatropha curcas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCA AGAATTTTCT GACATCGGTT TTACCGGTTG TTCTAATATT TGCTTCCTAT 
TTTTTTGAAA GGGGATGCTG TGCTAATGTG AGTTATGATC ACAGAGCACT TATTATTGAT 
GGAAAAAGAC GAGTTTTGCA GTCTGGTTCC ATACATTATC CAAGAACTAC TCCTGAGGTG 
AAATTTCAGG TGTGGCCAGA TATTATTAAG AAGTCGAAGG AGGGGGGATT GGACGTGATT 
GAGACTTATG TCTTCTGGAA TTACCATGAA CCCGTGAGAG GCCAGTACTA TTTTGAAGGC 
AGGTTTGACC TGGTGAGTTT TGTAAAAACA GTTCAGGAAG CTGGTCTTTT TGTTCATCTG 
AGGATTGGGC CATATGCCTG TGCTGAATGG AATTATGGGG GATTTCCAAT CTGGTTACAC 
TTCATTCCTG GGATTCAGTT TCGAACAACA AATGGACCTT TTAAGGAGGA AATGAAGCGT 
TTTCTTGGAA AGATTGTAAC TTTAATGAAG GAAGCGAATC TCTTTGCATC ACAAGGAGGG 
CCAATTATTC TTGCTCAGGT TGAGAATGAA TATGGGAATG TTGAATGGGC ATACGGGGTT 
GCAGGAGAGT TATATGTCAA ATGGGCCGCT GAAACTGCTG TTAGTCTGAA TACAAGTGTA 
CCTTGGGTGA TGTGCCAACA AGAAGACGCC CCTGATCCAA TTATAAATAC ATGTAATGGA 
TTCTACTGTG ATCAGTTTAC CCCAAATTCT CCTTCCAAAC CAAAAATCTG GACTGAGAAC 
TATAGTGGCT GGTTTCTGTC ATTTGGTTAT GCAATTCCTC ATCGACCTGT TGAGGACCTA 
GCTTTTGCTG TTGCACGCTT TTTTGAATAT GGTGGTACTT TCCAAAACTA CTATATGTAT 
TTTGGTGGAA CCAACTTTGG AAGAACAGCT GGTGGCCCTT TGGTTGCAAC AAGCTATGAT 
TATGATGCCC CAATAGATGA ATATGGTTTC ATCAGACAGC CAAAGTGGGG TCATCTACGC 
GACTTGCACA AATCAATAAA ACAGTGTGAA GACCATTTGG TCAGCTCAGA CCCAACACAC 
CTGAAGCTTG GTTATAACTT AGAGGCACAT ATCTACTATA AGTCTTCCAA TGATTGTGCT 
GCTTTTCTTG CTAATTATGA CAGCAATTCA GATGCACATG TTACATTCAA TGGACATATG 
TACTTTCTTC CTGCTTGGTC AGTGAGCATA CTTCCAGATT GCAAGAATGT CATTTTTAAC 
ACTGCCAAGG TCGTGTCCCA AAGAAATCTT GACGATGCTT CATTTGCTCA TTCAACCTCT 
AGTAAAGAGA TTTCACTGGA ACAAACAGCG TGGAGTTGGT ACAAAGAAAA AGTGGGCAGT 
TGGGGCAACA ACTCATTTAT AAAGCCAGAT TTATTGGAGC AGATTAATAC AACTAAAGAT 
GCCAGTGATT TTCTATGGTA CTCGACCAGT ATTAATGTGA ATGCTGGTCA AGAGAAAGAT 
TTCATTTTGA ACATTGAGAG TTTGGGGCAT GCAGCACTTG TTTTTGTAAA CAAAAGGTTT 
GTTGCATTTG CATACGGTAA TCATGATTTG GCAAGCTTTT CACTCACTAA GAAAATTAGT 
CTTGATGAAG GAAACAACAC ACTGGATTTA TTAAGCATGT TGGTCGGCCT ACAGAATTAT 
GGACCCTGGT TTGATGTTCA AGGAGCTGGA ATTTATTCCG TTACACTAAT TGGCCCGAGT 
AAAGGCAAGA ACGATCTTTC TTCTGGAAAA TGGACCTATC AGGTGGGACT GGAAGGGGAA 
TACCTTGGAC TGGATAAGAT CAGTCAAGCA AATAGTTCAC TGTGGACTAA AGGTCCTTCT 
CTGCCTGTTA ATAAGAGTTT GATATGGTAC AAGGATACTT TCATCGTCCC TGAAGGAAAG 
GGCCCTCTAG CCTTAAATCT AACAAGCATG GGAAAGGGTC AGGCCTGGGT TAATGGACAC 
AGCATAGGAC GATATTGGCC TGCATATCTC TCACCTTCAA CAGGATGCAC AGAAAATTGT 
GATTACAGAG GAACTTATGA TCCATCTAAA TGTCTGAAGA AATGTGGTCA GCCTGCTCAA 
ACACTATATC ATATCCCACG AACATGGATC CAGCCTGGAG AAAACCTACT AGTCCTACAT 
GAAGAGCTTG GCGGCGATCC TTCAAAGATT TCTGTGCTAA CAAGAACTGG CCAAGAGATA 
TGTTCAATTG TGTCAGAAGA TGATCCACCA CCAGCTGAGT CTTGGAAACC AAGCTTAGAC 
TTCAAGTCTC AAAGCCCTGA AATTCGATTG ACATGTGAAC AAGGATGGCG AATTACAACA 
ATCAATTTTG CTAGTTTTGG AACTCCTGCT GGATACTGTG GCTCATTCAA TCTAGGAAAC 
TGTCATGCTG ACATGTTATC CATTGTTCAA AAGGCTTGCA TAGGTCGAGA AAGGTGTTCA 
ATCTCCATAT CAGCAGCCAA TCTTGGTGAT CCTTGCCCTG GAGTTCTGAA AAGACTTGCT 
GTTGAAGCTT TTTGCAGTGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020541248.1
CDS	153..2696
Translation	MGSKNFLTSVLPVVLIFASYFFERGCCANVSYDHRALIIDGKRRVLQSGSIHYPRTTPEVKFQVWPDIIKKSKEGGLDVIETYVFWNYHEPVRGQYYFEGRFDLVSFVKTVQEAGLFVHLRIGPYACAEWNYGGFPIWLHFIPGIQFRTTNGPFKEEMKRFLGKIVTLMKEANLFASQGGPIILAQVENEYGNVEWAYGVAGELYVKWAAETAVSLNTSVPWVMCQQEDAPDPIINTCNGFYCDQFTPNSPSKPKIWTENYSGWFLSFGYAIPHRPVEDLAFAVARFFEYGGTFQNYYMYFGGTNFGRTAGGPLVATSYDYDAPIDEYGFIRQPKWGHLRDLHKSIKQCEDHLVSSDPTHLKLGYNLEAHIYYKSSNDCAAFLANYDSNSDAHVTFNGHMYFLPAWSVSILPDCKNVIFNTAKVVSQRNLDDASFAHSTSSKEISLEQTAWSWYKEKVGSWGNNSFIKPDLLEQINTTKDASDFLWYSTSINVNAGQEKDFILNIESLGHAALVFVNKRFVAFAYGNHDLASFSLTKKISLDEGNNTLDLLSMLVGLQNYGPWFDVQGAGIYSVTLIGPSKGKNDLSSGKWTYQVGLEGEYLGLDKISQANSSLWTKGPSLPVNKSLIWYKDTFIVPEGKGPLALNLTSMGKGQAWVNGHSIGRYWPAYLSPSTGCTENCDYRGTYDPSKCLKKCGQPAQTLYHIPRTWIQPGENLLVLHEELGGDPSKISVLTRTGQEICSIVSEDDPPPAESWKPSLDFKSQSPEIRLTCEQGWRITTINFASFGTPAGYCGSFNLGNCHADMLSIVQKACIGRERCSISISAANLGDPCPGVLKRLAVEAFCSD

Target ORF information:

RefSeq Version	XM_020685589.1
Organism	Jatropha curcas
Definition	Jatropha curcas beta-galactosidase 8 (LOC105649477), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020685589.1

ORF Insert Sequence:

ATGGGGAGCA AGAATTTTCT GACATCGGTT TTACCGGTTG TTCTAATATT TGCTTCCTAT 
TTTTTTGAAA GGGGATGCTG TGCTAATGTG AGTTATGATC ACAGAGCACT TATTATTGAT 
GGAAAAAGAC GAGTTTTGCA GTCTGGTTCC ATACATTATC CAAGAACTAC TCCTGAGGTG 
AAATTTCAGG TGTGGCCAGA TATTATTAAG AAGTCGAAGG AGGGGGGATT GGACGTGATT 
GAGACTTATG TCTTCTGGAA TTACCATGAA CCCGTGAGAG GCCAGTACTA TTTTGAAGGC 
AGGTTTGACC TGGTGAGTTT TGTAAAAACA GTTCAGGAAG CTGGTCTTTT TGTTCATCTG 
AGGATTGGGC CATATGCCTG TGCTGAATGG AATTATGGGG GATTTCCAAT CTGGTTACAC 
TTCATTCCTG GGATTCAGTT TCGAACAACA AATGGACCTT TTAAGGAGGA AATGAAGCGT 
TTTCTTGGAA AGATTGTAAC TTTAATGAAG GAAGCGAATC TCTTTGCATC ACAAGGAGGG 
CCAATTATTC TTGCTCAGGT TGAGAATGAA TATGGGAATG TTGAATGGGC ATACGGGGTT 
GCAGGAGAGT TATATGTCAA ATGGGCCGCT GAAACTGCTG TTAGTCTGAA TACAAGTGTA 
CCTTGGGTGA TGTGCCAACA AGAAGACGCC CCTGATCCAA TTATAAATAC ATGTAATGGA 
TTCTACTGTG ATCAGTTTAC CCCAAATTCT CCTTCCAAAC CAAAAATCTG GACTGAGAAC 
TATAGTGGCT GGTTTCTGTC ATTTGGTTAT GCAATTCCTC ATCGACCTGT TGAGGACCTA 
GCTTTTGCTG TTGCACGCTT TTTTGAATAT GGTGGTACTT TCCAAAACTA CTATATGTAT 
TTTGGTGGAA CCAACTTTGG AAGAACAGCT GGTGGCCCTT TGGTTGCAAC AAGCTATGAT 
TATGATGCCC CAATAGATGA ATATGGTTTC ATCAGACAGC CAAAGTGGGG TCATCTACGC 
GACTTGCACA AATCAATAAA ACAGTGTGAA GACCATTTGG TCAGCTCAGA CCCAACACAC 
CTGAAGCTTG GTTATAACTT AGAGGCACAT ATCTACTATA AGTCTTCCAA TGATTGTGCT 
GCTTTTCTTG CTAATTATGA CAGCAATTCA GATGCACATG TTACATTCAA TGGACATATG 
TACTTTCTTC CTGCTTGGTC AGTGAGCATA CTTCCAGATT GCAAGAATGT CATTTTTAAC 
ACTGCCAAGG TCGTGTCCCA AAGAAATCTT GACGATGCTT CATTTGCTCA TTCAACCTCT 
AGTAAAGAGA TTTCACTGGA ACAAACAGCG TGGAGTTGGT ACAAAGAAAA AGTGGGCAGT 
TGGGGCAACA ACTCATTTAT AAAGCCAGAT TTATTGGAGC AGATTAATAC AACTAAAGAT 
GCCAGTGATT TTCTATGGTA CTCGACCAGT ATTAATGTGA ATGCTGGTCA AGAGAAAGAT 
TTCATTTTGA ACATTGAGAG TTTGGGGCAT GCAGCACTTG TTTTTGTAAA CAAAAGGTTT 
GTTGCATTTG CATACGGTAA TCATGATTTG GCAAGCTTTT CACTCACTAA GAAAATTAGT 
CTTGATGAAG GAAACAACAC ACTGGATTTA TTAAGCATGT TGGTCGGCCT ACAGAATTAT 
GGACCCTGGT TTGATGTTCA AGGAGCTGGA ATTTATTCCG TTACACTAAT TGGCCCGAGT 
AAAGGCAAGA ACGATCTTTC TTCTGGAAAA TGGACCTATC AGGTGGGACT GGAAGGGGAA 
TACCTTGGAC TGGATAAGAT CAGTCAAGCA AATAGTTCAC TGTGGACTAA AGGTCCTTCT 
CTGCCTGTTA ATAAGAGTTT GATATGGTAC AAGGATACTT TCATCGTCCC TGAAGGAAAG 
GGCCCTCTAG CCTTAAATCT AACAAGCATG GGAAAGGGTC AGGCCTGGGT TAATGGACAC 
AGCATAGGAC GATATTGGCC TGCATATCTC TCACCTTCAA CAGGATGCAC AGAAAATTGT 
GATTACAGAG GAACTTATGA TCCATCTAAA TGTCTGAAGA AATGTGGTCA GCCTGCTCAA 
ACACTATATC ATATCCCACG AACATGGATC CAGCCTGGAG AAAACCTACT AGTCCTACAT 
GAAGAGCTTG GCGGCGATCC TTCAAAGATT TCTGTGCTAA CAAGAACTGG CCAAGAGATA 
TGTTCAATTG TGTCAGAAGA TGATCCACCA CCAGCTGAGT CTTGGAAACC AAGCTTAGAC 
TTCAAGTCTC AAAGCCCTGA AATTCGATTG ACATGTGAAC AAGGATGGCG AATTACAACA 
ATCAATTTTG CTAGTTTTGG AACTCCTGCT GGATACTGTG GCTCATTCAA TCTAGGAAAC 
TGTCATGCTG ACATGTTATC CATTGTTCAA AAGGCTTGCA TAGGTCGAGA AAGGTGTTCA 
ATCTCCATAT CAGCAGCCAA TCTTGGTGAT CCTTGCCCTG GAGTTCTGAA AAGACTTGCT 
GTTGAAGCTT TTTGCAGTGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105649477 ( XM_012236128.2 ) cDNA ORF clone, Jatropha curcas -> XP_012091518.1 Jatropha curcas beta-galactosidase 8 (LOC105649477), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OJa66114
Clone ID Related Accession (Same CDS sequence)	XM_012236128.2
Accession Version	XM_012236128.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-05
Organism	Jatropha curcas
Product	beta-galactosidase 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012125517.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 6, 2017 this sequence version replaced XM_012236128.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Jatropha curcas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCA AGAATTTTCT GACATCGGTT TTACCGGTTG TTCTAATATT TGCTTCCTAT 
TTTTTTGAAA GGGGATGCTG TGCTAATGTG AGTTATGATC ACAGAGCACT TATTATTGAT 
GGAAAAAGAC GAGTTTTGCA GTCTGGTTCC ATACATTATC CAAGAACTAC TCCTGAGGTG 
TGGCCAGATA TTATTAAGAA GTCGAAGGAG GGGGGATTGG ACGTGATTGA GACTTATGTC 
TTCTGGAATT ACCATGAACC CGTGAGAGGC CAGTACTATT TTGAAGGCAG GTTTGACCTG 
GTGAGTTTTG TAAAAACAGT TCAGGAAGCT GGTCTTTTTG TTCATCTGAG GATTGGGCCA 
TATGCCTGTG CTGAATGGAA TTATGGGGGA TTTCCAATCT GGTTACACTT CATTCCTGGG 
ATTCAGTTTC GAACAACAAA TGGACCTTTT AAGGAGGAAA TGAAGCGTTT TCTTGGAAAG 
ATTGTAACTT TAATGAAGGA AGCGAATCTC TTTGCATCAC AAGGAGGGCC AATTATTCTT 
GCTCAGGTTG AGAATGAATA TGGGAATGTT GAATGGGCAT ACGGGGTTGC AGGAGAGTTA 
TATGTCAAAT GGGCCGCTGA AACTGCTGTT AGTCTGAATA CAAGTGTACC TTGGGTGATG 
TGCCAACAAG AAGACGCCCC TGATCCAATT ATAAATACAT GTAATGGATT CTACTGTGAT 
CAGTTTACCC CAAATTCTCC TTCCAAACCA AAAATCTGGA CTGAGAACTA TAGTGGCTGG 
TTTCTGTCAT TTGGTTATGC AATTCCTCAT CGACCTGTTG AGGACCTAGC TTTTGCTGTT 
GCACGCTTTT TTGAATATGG TGGTACTTTC CAAAACTACT ATATGTATTT TGGTGGAACC 
AACTTTGGAA GAACAGCTGG TGGCCCTTTG GTTGCAACAA GCTATGATTA TGATGCCCCA 
ATAGATGAAT ATGGTTTCAT CAGACAGCCA AAGTGGGGTC ATCTACGCGA CTTGCACAAA 
TCAATAAAAC AGTGTGAAGA CCATTTGGTC AGCTCAGACC CAACACACCT GAAGCTTGGT 
TATAACTTAG AGGCACATAT CTACTATAAG TCTTCCAATG ATTGTGCTGC TTTTCTTGCT 
AATTATGACA GCAATTCAGA TGCACATGTT ACATTCAATG GACATATGTA CTTTCTTCCT 
GCTTGGTCAG TGAGCATACT TCCAGATTGC AAGAATGTCA TTTTTAACAC TGCCAAGGTC 
GTGTCCCAAA GAAATCTTGA CGATGCTTCA TTTGCTCATT CAACCTCTAG TAAAGAGATT 
TCACTGGAAC AAACAGCGTG GAGTTGGTAC AAAGAAAAAG TGGGCAGTTG GGGCAACAAC 
TCATTTATAA AGCCAGATTT ATTGGAGCAG ATTAATACAA CTAAAGATGC CAGTGATTTT 
CTATGGTACT CGACCAGTAT TAATGTGAAT GCTGGTCAAG AGAAAGATTT CATTTTGAAC 
ATTGAGAGTT TGGGGCATGC AGCACTTGTT TTTGTAAACA AAAGGTTTGT TGCATTTGCA 
TACGGTAATC ATGATTTGGC AAGCTTTTCA CTCACTAAGA AAATTAGTCT TGATGAAGGA 
AACAACACAC TGGATTTATT AAGCATGTTG GTCGGCCTAC AGAATTATGG ACCCTGGTTT 
GATGTTCAAG GAGCTGGAAT TTATTCCGTT ACACTAATTG GCCCGAGTAA AGGCAAGAAC 
GATCTTTCTT CTGGAAAATG GACCTATCAG GTGGGACTGG AAGGGGAATA CCTTGGACTG 
GATAAGATCA GTCAAGCAAA TAGTTCACTG TGGACTAAAG GTCCTTCTCT GCCTGTTAAT 
AAGAGTTTGA TATGGTACAA GGATACTTTC ATCGTCCCTG AAGGAAAGGG CCCTCTAGCC 
TTAAATCTAA CAAGCATGGG AAAGGGTCAG GCCTGGGTTA ATGGACACAG CATAGGACGA 
TATTGGCCTG CATATCTCTC ACCTTCAACA GGATGCACAG AAAATTGTGA TTACAGAGGA 
ACTTATGATC CATCTAAATG TCTGAAGAAA TGTGGTCAGC CTGCTCAAAC ACTATATCAT 
ATCCCACGAA CATGGATCCA GCCTGGAGAA AACCTACTAG TCCTACATGA AGAGCTTGGC 
GGCGATCCTT CAAAGATTTC TGTGCTAACA AGAACTGGCC AAGAGATATG TTCAATTGTG 
TCAGAAGATG ATCCACCACC AGCTGAGTCT TGGAAACCAA GCTTAGACTT CAAGTCTCAA 
AGCCCTGAAA TTCGATTGAC ATGTGAACAA GGATGGCGAA TTACAACAAT CAATTTTGCT 
AGTTTTGGAA CTCCTGCTGG ATACTGTGGC TCATTCAATC TAGGAAACTG TCATGCTGAC 
ATGTTATCCA TTGTTCAAAA GGCTTGCATA GGTCGAGAAA GGTGTTCAAT CTCCATATCA 
GCAGCCAATC TTGGTGATCC TTGCCCTGGA GTTCTGAAAA GACTTGCTGT TGAAGCTTTT 
TGCAGTGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012091518.1
CDS	152..2683
Translation	MGSKNFLTSVLPVVLIFASYFFERGCCANVSYDHRALIIDGKRRVLQSGSIHYPRTTPEVWPDIIKKSKEGGLDVIETYVFWNYHEPVRGQYYFEGRFDLVSFVKTVQEAGLFVHLRIGPYACAEWNYGGFPIWLHFIPGIQFRTTNGPFKEEMKRFLGKIVTLMKEANLFASQGGPIILAQVENEYGNVEWAYGVAGELYVKWAAETAVSLNTSVPWVMCQQEDAPDPIINTCNGFYCDQFTPNSPSKPKIWTENYSGWFLSFGYAIPHRPVEDLAFAVARFFEYGGTFQNYYMYFGGTNFGRTAGGPLVATSYDYDAPIDEYGFIRQPKWGHLRDLHKSIKQCEDHLVSSDPTHLKLGYNLEAHIYYKSSNDCAAFLANYDSNSDAHVTFNGHMYFLPAWSVSILPDCKNVIFNTAKVVSQRNLDDASFAHSTSSKEISLEQTAWSWYKEKVGSWGNNSFIKPDLLEQINTTKDASDFLWYSTSINVNAGQEKDFILNIESLGHAALVFVNKRFVAFAYGNHDLASFSLTKKISLDEGNNTLDLLSMLVGLQNYGPWFDVQGAGIYSVTLIGPSKGKNDLSSGKWTYQVGLEGEYLGLDKISQANSSLWTKGPSLPVNKSLIWYKDTFIVPEGKGPLALNLTSMGKGQAWVNGHSIGRYWPAYLSPSTGCTENCDYRGTYDPSKCLKKCGQPAQTLYHIPRTWIQPGENLLVLHEELGGDPSKISVLTRTGQEICSIVSEDDPPPAESWKPSLDFKSQSPEIRLTCEQGWRITTINFASFGTPAGYCGSFNLGNCHADMLSIVQKACIGRERCSISISAANLGDPCPGVLKRLAVEAFCSD

Target ORF information:

RefSeq Version	XM_012236128.2
Organism	Jatropha curcas
Definition	Jatropha curcas beta-galactosidase 8 (LOC105649477), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012236128.2

ORF Insert Sequence:

ATGGGGAGCA AGAATTTTCT GACATCGGTT TTACCGGTTG TTCTAATATT TGCTTCCTAT 
TTTTTTGAAA GGGGATGCTG TGCTAATGTG AGTTATGATC ACAGAGCACT TATTATTGAT 
GGAAAAAGAC GAGTTTTGCA GTCTGGTTCC ATACATTATC CAAGAACTAC TCCTGAGGTG 
TGGCCAGATA TTATTAAGAA GTCGAAGGAG GGGGGATTGG ACGTGATTGA GACTTATGTC 
TTCTGGAATT ACCATGAACC CGTGAGAGGC CAGTACTATT TTGAAGGCAG GTTTGACCTG 
GTGAGTTTTG TAAAAACAGT TCAGGAAGCT GGTCTTTTTG TTCATCTGAG GATTGGGCCA 
TATGCCTGTG CTGAATGGAA TTATGGGGGA TTTCCAATCT GGTTACACTT CATTCCTGGG 
ATTCAGTTTC GAACAACAAA TGGACCTTTT AAGGAGGAAA TGAAGCGTTT TCTTGGAAAG 
ATTGTAACTT TAATGAAGGA AGCGAATCTC TTTGCATCAC AAGGAGGGCC AATTATTCTT 
GCTCAGGTTG AGAATGAATA TGGGAATGTT GAATGGGCAT ACGGGGTTGC AGGAGAGTTA 
TATGTCAAAT GGGCCGCTGA AACTGCTGTT AGTCTGAATA CAAGTGTACC TTGGGTGATG 
TGCCAACAAG AAGACGCCCC TGATCCAATT ATAAATACAT GTAATGGATT CTACTGTGAT 
CAGTTTACCC CAAATTCTCC TTCCAAACCA AAAATCTGGA CTGAGAACTA TAGTGGCTGG 
TTTCTGTCAT TTGGTTATGC AATTCCTCAT CGACCTGTTG AGGACCTAGC TTTTGCTGTT 
GCACGCTTTT TTGAATATGG TGGTACTTTC CAAAACTACT ATATGTATTT TGGTGGAACC 
AACTTTGGAA GAACAGCTGG TGGCCCTTTG GTTGCAACAA GCTATGATTA TGATGCCCCA 
ATAGATGAAT ATGGTTTCAT CAGACAGCCA AAGTGGGGTC ATCTACGCGA CTTGCACAAA 
TCAATAAAAC AGTGTGAAGA CCATTTGGTC AGCTCAGACC CAACACACCT GAAGCTTGGT 
TATAACTTAG AGGCACATAT CTACTATAAG TCTTCCAATG ATTGTGCTGC TTTTCTTGCT 
AATTATGACA GCAATTCAGA TGCACATGTT ACATTCAATG GACATATGTA CTTTCTTCCT 
GCTTGGTCAG TGAGCATACT TCCAGATTGC AAGAATGTCA TTTTTAACAC TGCCAAGGTC 
GTGTCCCAAA GAAATCTTGA CGATGCTTCA TTTGCTCATT CAACCTCTAG TAAAGAGATT 
TCACTGGAAC AAACAGCGTG GAGTTGGTAC AAAGAAAAAG TGGGCAGTTG GGGCAACAAC 
TCATTTATAA AGCCAGATTT ATTGGAGCAG ATTAATACAA CTAAAGATGC CAGTGATTTT 
CTATGGTACT CGACCAGTAT TAATGTGAAT GCTGGTCAAG AGAAAGATTT CATTTTGAAC 
ATTGAGAGTT TGGGGCATGC AGCACTTGTT TTTGTAAACA AAAGGTTTGT TGCATTTGCA 
TACGGTAATC ATGATTTGGC AAGCTTTTCA CTCACTAAGA AAATTAGTCT TGATGAAGGA 
AACAACACAC TGGATTTATT AAGCATGTTG GTCGGCCTAC AGAATTATGG ACCCTGGTTT 
GATGTTCAAG GAGCTGGAAT TTATTCCGTT ACACTAATTG GCCCGAGTAA AGGCAAGAAC 
GATCTTTCTT CTGGAAAATG GACCTATCAG GTGGGACTGG AAGGGGAATA CCTTGGACTG 
GATAAGATCA GTCAAGCAAA TAGTTCACTG TGGACTAAAG GTCCTTCTCT GCCTGTTAAT 
AAGAGTTTGA TATGGTACAA GGATACTTTC ATCGTCCCTG AAGGAAAGGG CCCTCTAGCC 
TTAAATCTAA CAAGCATGGG AAAGGGTCAG GCCTGGGTTA ATGGACACAG CATAGGACGA 
TATTGGCCTG CATATCTCTC ACCTTCAACA GGATGCACAG AAAATTGTGA TTACAGAGGA 
ACTTATGATC CATCTAAATG TCTGAAGAAA TGTGGTCAGC CTGCTCAAAC ACTATATCAT 
ATCCCACGAA CATGGATCCA GCCTGGAGAA AACCTACTAG TCCTACATGA AGAGCTTGGC 
GGCGATCCTT CAAAGATTTC TGTGCTAACA AGAACTGGCC AAGAGATATG TTCAATTGTG 
TCAGAAGATG ATCCACCACC AGCTGAGTCT TGGAAACCAA GCTTAGACTT CAAGTCTCAA 
AGCCCTGAAA TTCGATTGAC ATGTGAACAA GGATGGCGAA TTACAACAAT CAATTTTGCT 
AGTTTTGGAA CTCCTGCTGG ATACTGTGGC TCATTCAATC TAGGAAACTG TCATGCTGAC 
ATGTTATCCA TTGTTCAAAA GGCTTGCATA GGTCGAGAAA GGTGTTCAAT CTCCATATCA 
GCAGCCAATC TTGGTGATCC TTGCCCTGGA GTTCTGAAAA GACTTGCTGT TGAAGCTTTT 
TGCAGTGATT AA