LOC105646047 cDNA ORF clone, Jatropha curcas

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105646047
Entrez Gene ID	105646047
Full Name	E3 ubiquitin-protein ligase RNF181 homolog
Gene Type	protein-coding
Organism	Jatropha curcas

mRNA and Protein(s)

mRNA	Protein	Name
XM_012231818.2	XP_012087208.1	E3 ubiquitin-protein ligase RNF181 homolog

Pathways from BioSystems
Gene Ontology

The following LOC105646047 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105646047 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OJa61933	XM_012231818.2 Latest version!	Jatropha curcas E3 ubiquitin-protein ligase RNF181 homolog (LOC105646047), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105646047 ( XM_012231818.2 ) cDNA ORF clone, Jatropha curcas -> XP_012087208.1 Jatropha curcas E3 ubiquitin-protein ligase RNF181 homolog (LOC105646047), mRNA.

Price & Availability↑

CloneID	OJa61933
Clone ID Related Accession (Same CDS sequence)	XM_012231818.2
Accession Version	XM_012231818.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-05
Organism	Jatropha curcas
Product	E3 ubiquitin-protein ligase RNF181 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012124848.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 6, 2017 this sequence version replaced XM_012231818.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Jatropha curcas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACT TGTCTCCGAG AGAAAGAGAT CGATTTATTC GAACTTGGCT ACATAGTATT 
ATTCCAGTTC AAACTGTACT GGATGATAAT ATCCAATCCA TGTTAGTAGA AGCTAATAGA 
GATTTCTCAC CGGAGCAATA CTTGGGAATC GGAGATGACG ACGAAGAAAC TATCTTTGGG 
GATGATGATG ATGAAGATTT GGTGATCGCA CTGTTAGCAA GCATTGATTA TTGTCCAACA 
GCGCAGCCAG CAAGCCAAGA ATCCATTGAT AAACTGAAAG ATGTGAGAAT TGAAAAATCG 
GATTTGGATT GTAGCATCTG CTTGGATGAA CTTTTGGTAG GGTCTGAAGC CAAGTGCTTG 
CCATGCTCAC ACGTTTACCA TGGAAATTGT ATCGCAGAGT GGCTGAAGAA TAGCAACACC 
TGTCCTCTCT GTCGGTATTC GTTACCCACA GATTCTTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012087208.1
CDS	431..892
Translation	MDNLSPRERDRFIRTWLHSIIPVQTVLDDNIQSMLVEANRDFSPEQYLGIGDDDEETIFGDDDDEDLVIALLASIDYCPTAQPASQESIDKLKDVRIEKSDLDCSICLDELLVGSEAKCLPCSHVYHGNCIAEWLKNSNTCPLCRYSLPTDSS

Target ORF information:

RefSeq Version	XM_012231818.2
Organism	Jatropha curcas
Definition	Jatropha curcas E3 ubiquitin-protein ligase RNF181 homolog (LOC105646047), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012231818.2

ORF Insert Sequence:

ATGGACAACT TGTCTCCGAG AGAAAGAGAT CGATTTATTC GAACTTGGCT ACATAGTATT 
ATTCCAGTTC AAACTGTACT GGATGATAAT ATCCAATCCA TGTTAGTAGA AGCTAATAGA 
GATTTCTCAC CGGAGCAATA CTTGGGAATC GGAGATGACG ACGAAGAAAC TATCTTTGGG 
GATGATGATG ATGAAGATTT GGTGATCGCA CTGTTAGCAA GCATTGATTA TTGTCCAACA 
GCGCAGCCAG CAAGCCAAGA ATCCATTGAT AAACTGAAAG ATGTGAGAAT TGAAAAATCG 
GATTTGGATT GTAGCATCTG CTTGGATGAA CTTTTGGTAG GGTCTGAAGC CAAGTGCTTG 
CCATGCTCAC ACGTTTACCA TGGAAATTGT ATCGCAGAGT GGCTGAAGAA TAGCAACACC 
TGTCCTCTCT GTCGGTATTC GTTACCCACA GATTCTTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.