LOC105631109 cDNA ORF clone, Jatropha curcas

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105631109
Entrez Gene ID	105631109
Full Name	TMV resistance protein N
Gene Type	protein-coding
Organism	Jatropha curcas

mRNA and Protein(s)

mRNA	Protein	Name
XM_012213111.2	XP_012068501.1	TMV resistance protein N isoform X2
XM_012213110.2	XP_012068500.1	TMV resistance protein N isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC105631109 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105631109 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OJa46008	XM_012213111.2 Latest version!	Jatropha curcas TMV resistance protein N (LOC105631109), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OJa46007	XM_012213110.2 Latest version!	Jatropha curcas TMV resistance protein N (LOC105631109), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105631109 ( XM_012213111.2 ) cDNA ORF clone, Jatropha curcas -> XP_012068501.1 Jatropha curcas TMV resistance protein N (LOC105631109), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OJa46008
Clone ID Related Accession (Same CDS sequence)	XM_012213111.2
Accession Version	XM_012213111.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-05
Organism	Jatropha curcas
Product	TMV resistance protein N isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012124178.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 6, 2017 this sequence version replaced XM_012213111.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Jatropha curcas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTA CTTCGTCCAC TGCTGTTCGC AGAAAATGCG ATGTGTTCCT TAATTTTAGA 
GGTGGAGACA CTTGCGAGAA CTTTGTCAGT GAACTCTGCA AAACTTTAAA TCGAAATCTG 
ATCGAGACTT TCATCATCGA TGATAAATTT GGTGGCAGAG GAGGAGAAGA AGAGATTCTG 
CGAAAAATAC AAGAAGCAGA TATTTCTTCA GTCATTTTCT CGGAAGACTA TGCTTCTTCT 
GCTTTGTGTA TGGATGAGCT CCTAAAGATA GTTGAATGTA AAGAGACGAA AGGGCAAACT 
GTGATACCAG TTTTTTACCG AGTAGATCCA ACGCATGTTC AACACCTGAA AGGAAATTTT 
GGGTTTGGAT TTGACAAACT TACTCAAGAA TTGAGGAACT CCTTGCCCAA AGTGGAGAGT 
TGGAGAAGTG CTTTGATTAA GACTGCATAT CTCAAGGGAT GGGATTCACA CTTCACCAAG 
CCTGAATCCA AGCTAATTCA TGAAATTGTG AATGATATTT TGAAGAAGTT GAATCAAGTG 
TTCTTCAATG GTTCTGTCTT AGTTGGAATT AGTTCACACA TCAGGGAAGT AGAATCATTA 
TTGTCCATTG AATCACAAGG TGTGCGCTCA ATAGGAATAT GGGGAATGGG AGGCATAGGC 
AAAACAACTA TTACTGAAGT TCTGTTTAGT CGAATTTCTG CTCAGTTTGA ATGTCAATGC 
TATGTCCCAA ATGTGAGGGA ACAACTGGAA AAGCGTACGG TAGCTCATAT AAGAGCTGAA 
ATTCTTTCTA AATTACTGGG AATGGAAAAT AGTATAGTCC GCATGCCCTA TATATTTCCA 
AATTTCATTA GGAATAAGCT TAGGAAGAAA AAAGTTCTCA TTGTTCTTGA TGATGTGGAT 
AATTCTGAGG TAGTAAGGGA TTTAGTAGGA GACTGTCATT TGTATGGTTC GGGAAGTAGA 
ATAATTGTAA CGAGTAGAGA CAAACAAGTA CTTAAAAATG CTGATATCAA GGAGTATATT 
TATGAGGTTG AGAAATTGGT TGACTTTCAA GCTATTCTAC TATTTAGCTT GCATGCTTTC 
AAACAAGATG CTCCACCAGA AGAATTTATG AAGCTATCAG AAAGGGCAAT AACCTATGCT 
CAAGGGATTC CATTAGCTAT TAAAATTTTA GGGTCCAGTC TATATTGCAA AAGTGTAAAT 
GAATGGGAAA GTGAATTGGC AAAACTTGAA AATAGCCCAG ACAGGACAAT TGAGGAAGTT 
CTGAAAATAA GTTATGATGG ATTAGAAAGC AGTGAACAAA GCATATTTCT TGATATTGCA 
TGCTTCTTTA AGGGAGAAGA TAGAACTCGC ATTCAAAGTA TATTGAATGG TTGCGGATTC 
TTTGCAGATA TTGGAATAAG TCGTCTCTTA GATAAGTCTC TGATAACTAT TTCATCTAAC 
AATCAGTTGG AAATGCATGA CCTACTACAA CAAATGGGAA GGAATATTGT TTGCTTGGAA 
TGCATTCATC AACCTGGAGA ACGCAGTAGA TTGTGGATTC CACAGGATGT CTATCTTGTA 
TTGAAAAGAG AAAAGGGGAC TGGAAACATT GAAGGAATAT CATTAGACAA GTCCAAAATC 
AGAGACATAG AACTAAGCCC AACAGTCTTT GAGAGAATGT ATAATCTTAG GTTGCTTGAA 
TTCCACAATC CATTCACAAG TGAATTGAAG ATATATCTTC CTAAAGGACT CCAGTTTCTT 
CCAGACGAGC TAAGATTTCT TCGTTGGGAC AAATACCCTT CAAAGTCTTT GCCAACAAGT 
TTTTGTGCTG AAAAGCTCAT GGAACTTCAC ATGCGTGAAA GCCAACTTAG AGAGCTTTGG 
AATGGAGTTC AGTCTCGCTA CATAGGTGCC AAGATGTATA TTGCTGGAAG TGAAATGGCA 
GAGAATATAA GGTATCACAC TAACAATGGA TCATCTCTGT CTTTCACAAT CGACCAGCAG 
CAGCCTGACC TGATTGGTTT CTCTTTATGT GCTGTGGTTG CATCCAATGG TCATCCTCCT 
CCTCATGACG TCATAAATAT TAGATGTAGA GCCCTTCTCA CAGGCGAGTC TGGACATAGC 
CATATTGAAG ATTTGTACTT TTTTAGTTAT CCAGATGAAC TAGACATCCA ATCGGAGACT 
CTGTTTCTGT GGGCTAATTC AATCTCTTGG GACTCCGAAG ACGGTTTGAG CAAGGCTTCA 
TTTCATTTCT TCATGGATTA TCATAATGAA GAAGACGAAG ACTATTATGA GTGCAAGGAG 
GTGATAGTTA GGTGTGGAGT TCATCCAATT TTCAAAGATA AGAAGAGCAA AAATAAAGAT 
GAAAATGAGG AATCTCAGCA ACCTCCTCTG AAAAGATTGA AAGAGGAACA TCTAAGCGCC 
GTCTCTTGGA TGACTACAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012068501.1
CDS	204..2627
Translation	MASTSSTAVRRKCDVFLNFRGGDTCENFVSELCKTLNRNLIETFIIDDKFGGRGGEEEILRKIQEADISSVIFSEDYASSALCMDELLKIVECKETKGQTVIPVFYRVDPTHVQHLKGNFGFGFDKLTQELRNSLPKVESWRSALIKTAYLKGWDSHFTKPESKLIHEIVNDILKKLNQVFFNGSVLVGISSHIREVESLLSIESQGVRSIGIWGMGGIGKTTITEVLFSRISAQFECQCYVPNVREQLEKRTVAHIRAEILSKLLGMENSIVRMPYIFPNFIRNKLRKKKVLIVLDDVDNSEVVRDLVGDCHLYGSGSRIIVTSRDKQVLKNADIKEYIYEVEKLVDFQAILLFSLHAFKQDAPPEEFMKLSERAITYAQGIPLAIKILGSSLYCKSVNEWESELAKLENSPDRTIEEVLKISYDGLESSEQSIFLDIACFFKGEDRTRIQSILNGCGFFADIGISRLLDKSLITISSNNQLEMHDLLQQMGRNIVCLECIHQPGERSRLWIPQDVYLVLKREKGTGNIEGISLDKSKIRDIELSPTVFERMYNLRLLEFHNPFTSELKIYLPKGLQFLPDELRFLRWDKYPSKSLPTSFCAEKLMELHMRESQLRELWNGVQSRYIGAKMYIAGSEMAENIRYHTNNGSSLSFTIDQQQPDLIGFSLCAVVASNGHPPPHDVINIRCRALLTGESGHSHIEDLYFFSYPDELDIQSETLFLWANSISWDSEDGLSKASFHFFMDYHNEEDEDYYECKEVIVRCGVHPIFKDKKSKNKDENEESQQPPLKRLKEEHLSAVSWMTTD

Target ORF information:

RefSeq Version	XM_012213111.2
Organism	Jatropha curcas
Definition	Jatropha curcas TMV resistance protein N (LOC105631109), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012213111.2

ORF Insert Sequence:

ATGGCATCTA CTTCGTCCAC TGCTGTTCGC AGAAAATGCG ATGTGTTCCT TAATTTTAGA 
GGTGGAGACA CTTGCGAGAA CTTTGTCAGT GAACTCTGCA AAACTTTAAA TCGAAATCTG 
ATCGAGACTT TCATCATCGA TGATAAATTT GGTGGCAGAG GAGGAGAAGA AGAGATTCTG 
CGAAAAATAC AAGAAGCAGA TATTTCTTCA GTCATTTTCT CGGAAGACTA TGCTTCTTCT 
GCTTTGTGTA TGGATGAGCT CCTAAAGATA GTTGAATGTA AAGAGACGAA AGGGCAAACT 
GTGATACCAG TTTTTTACCG AGTAGATCCA ACGCATGTTC AACACCTGAA AGGAAATTTT 
GGGTTTGGAT TTGACAAACT TACTCAAGAA TTGAGGAACT CCTTGCCCAA AGTGGAGAGT 
TGGAGAAGTG CTTTGATTAA GACTGCATAT CTCAAGGGAT GGGATTCACA CTTCACCAAG 
CCTGAATCCA AGCTAATTCA TGAAATTGTG AATGATATTT TGAAGAAGTT GAATCAAGTG 
TTCTTCAATG GTTCTGTCTT AGTTGGAATT AGTTCACACA TCAGGGAAGT AGAATCATTA 
TTGTCCATTG AATCACAAGG TGTGCGCTCA ATAGGAATAT GGGGAATGGG AGGCATAGGC 
AAAACAACTA TTACTGAAGT TCTGTTTAGT CGAATTTCTG CTCAGTTTGA ATGTCAATGC 
TATGTCCCAA ATGTGAGGGA ACAACTGGAA AAGCGTACGG TAGCTCATAT AAGAGCTGAA 
ATTCTTTCTA AATTACTGGG AATGGAAAAT AGTATAGTCC GCATGCCCTA TATATTTCCA 
AATTTCATTA GGAATAAGCT TAGGAAGAAA AAAGTTCTCA TTGTTCTTGA TGATGTGGAT 
AATTCTGAGG TAGTAAGGGA TTTAGTAGGA GACTGTCATT TGTATGGTTC GGGAAGTAGA 
ATAATTGTAA CGAGTAGAGA CAAACAAGTA CTTAAAAATG CTGATATCAA GGAGTATATT 
TATGAGGTTG AGAAATTGGT TGACTTTCAA GCTATTCTAC TATTTAGCTT GCATGCTTTC 
AAACAAGATG CTCCACCAGA AGAATTTATG AAGCTATCAG AAAGGGCAAT AACCTATGCT 
CAAGGGATTC CATTAGCTAT TAAAATTTTA GGGTCCAGTC TATATTGCAA AAGTGTAAAT 
GAATGGGAAA GTGAATTGGC AAAACTTGAA AATAGCCCAG ACAGGACAAT TGAGGAAGTT 
CTGAAAATAA GTTATGATGG ATTAGAAAGC AGTGAACAAA GCATATTTCT TGATATTGCA 
TGCTTCTTTA AGGGAGAAGA TAGAACTCGC ATTCAAAGTA TATTGAATGG TTGCGGATTC 
TTTGCAGATA TTGGAATAAG TCGTCTCTTA GATAAGTCTC TGATAACTAT TTCATCTAAC 
AATCAGTTGG AAATGCATGA CCTACTACAA CAAATGGGAA GGAATATTGT TTGCTTGGAA 
TGCATTCATC AACCTGGAGA ACGCAGTAGA TTGTGGATTC CACAGGATGT CTATCTTGTA 
TTGAAAAGAG AAAAGGGGAC TGGAAACATT GAAGGAATAT CATTAGACAA GTCCAAAATC 
AGAGACATAG AACTAAGCCC AACAGTCTTT GAGAGAATGT ATAATCTTAG GTTGCTTGAA 
TTCCACAATC CATTCACAAG TGAATTGAAG ATATATCTTC CTAAAGGACT CCAGTTTCTT 
CCAGACGAGC TAAGATTTCT TCGTTGGGAC AAATACCCTT CAAAGTCTTT GCCAACAAGT 
TTTTGTGCTG AAAAGCTCAT GGAACTTCAC ATGCGTGAAA GCCAACTTAG AGAGCTTTGG 
AATGGAGTTC AGTCTCGCTA CATAGGTGCC AAGATGTATA TTGCTGGAAG TGAAATGGCA 
GAGAATATAA GGTATCACAC TAACAATGGA TCATCTCTGT CTTTCACAAT CGACCAGCAG 
CAGCCTGACC TGATTGGTTT CTCTTTATGT GCTGTGGTTG CATCCAATGG TCATCCTCCT 
CCTCATGACG TCATAAATAT TAGATGTAGA GCCCTTCTCA CAGGCGAGTC TGGACATAGC 
CATATTGAAG ATTTGTACTT TTTTAGTTAT CCAGATGAAC TAGACATCCA ATCGGAGACT 
CTGTTTCTGT GGGCTAATTC AATCTCTTGG GACTCCGAAG ACGGTTTGAG CAAGGCTTCA 
TTTCATTTCT TCATGGATTA TCATAATGAA GAAGACGAAG ACTATTATGA GTGCAAGGAG 
GTGATAGTTA GGTGTGGAGT TCATCCAATT TTCAAAGATA AGAAGAGCAA AAATAAAGAT 
GAAAATGAGG AATCTCAGCA ACCTCCTCTG AAAAGATTGA AAGAGGAACA TCTAAGCGCC 
GTCTCTTGGA TGACTACAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105631109 ( XM_012213110.2 ) cDNA ORF clone, Jatropha curcas -> XP_012068500.1 Jatropha curcas TMV resistance protein N (LOC105631109), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa46007
Clone ID Related Accession (Same CDS sequence)	XM_012213110.2
Accession Version	XM_012213110.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-05
Organism	Jatropha curcas
Product	TMV resistance protein N isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012124178.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 6, 2017 this sequence version replaced XM_012213110.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Jatropha curcas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTA CTTCGTCCAC TGCTGTTCGC AGAAAATGCG ATGTGTTCCT TAATTTTAGA 
GGTGGAGACA CTTGCGAGAA CTTTGTCAGT GAACTCTGCA AAACTTTAAA TCGAAATCTG 
ATCGAGACTT TCATCATCGA TGATAAATTT GGTGGCAGAG GAGGAGAAGA AGAGATTCTG 
CGAAAAATAC AAGAAGCAGA TATTTCTTCA GTCATTTTCT CGGAAGACTA TGCTTCTTCT 
GCTTTGTGTA TGGATGAGCT CCTAAAGATA GTTGAATGTA AAGAGACGAA AGGGCAAACT 
GTGATACCAG TTTTTTACCG AGTAGATCCA ACGCATGTTC AACACCTGAA AGGAAATTTT 
GGGTTTGGAT TTGACAAACT TACTCAAGAA TTGAGGAACT CCTTGCCCAA AGTGGAGAGT 
TGGAGAAGTG CTTTGATTAA GACTGCATAT CTCAAGGGAT GGGATTCACA CTTCACCAAG 
CCTGAATCCA AGCTAATTCA TGAAATTGTG AATGATATTT TGAAGAAGTT GAATCAAGTG 
TTCTTCAATG GTTCTGTCTT AGTTGGAATT AGTTCACACA TCAGGGAAGT AGAATCATTA 
TTGTCCATTG AATCACAAGG TGTGCGCTCA ATAGGAATAT GGGGAATGGG AGGCATAGGC 
AAAACAACTA TTACTGAAGT TCTGTTTAGT CGAATTTCTG CTCAGTTTGA ATGTCAATGC 
TATGTCCCAA ATGTGAGGGA ACAACTGGAA AAGCGTACGG TAGCTCATAT AAGAGCTGAA 
ATTCTTTCTA AATTACTGGG AATGGAAAAT AGTATAGTCC GCATGCCCTA TATATTTCCA 
AATTTCATTA GGAATAAGCT TAGGAAGAAA AAAGTTCTCA TTGTTCTTGA TGATGTGGAT 
AATTCTGAGG TAGTAAGGGA TTTAGTAGGA GACTGTCATT TGTATGGTTC GGGAAGTAGA 
ATAATTGTAA CGAGTAGAGA CAAACAAGTA CTTAAAAATG CTGATATCAA GGAGTATATT 
TATGAGGTTG AGAAATTGGT TGACTTTCAA GCTATTCTAC TATTTAGCTT GCATGCTTTC 
AAACAAGATG CTCCACCAGA AGAATTTATG AAGCTATCAG AAAGGGCAAT AACCTATGCT 
CAAGGGATTC CATTAGCTAT TAAAATTTTA GGGTCCAGTC TATATTGCAA AAGTGTAAAT 
GAATGGGAAA GTGAATTGGC AAAACTTGAA AATAGCCCAG ACAGGACAAT TGAGGAAGTT 
CTGAAAATAA GTTATGATGG ATTAGAAAGC AGTGAACAAA GCATATTTCT TGATATTGCA 
TGCTTCTTTA AGGGAGAAGA TAGAACTCGC ATTCAAAGTA TATTGAATGG TTGCGGATTC 
TTTGCAGATA TTGGAATAAG TCGTCTCTTA GATAAGTCTC TGATAACTAT TTCATCTAAC 
AATCAGTTGG AAATGCATGA CCTACTACAA CAAATGGGAA GGAATATTGT TTGCTTGGAA 
TGCATTCATC AACCTGGAGA ACGCAGTAGA TTGTGGATTC CACAGGATGT CTATCTTGTA 
TTGAAAAGAG AAAAGGGGAC TGGAAACATT GAAGGAATAT CATTAGACAA GTCCAAAATC 
AGAGACATAG AACTAAGCCC AACAGTCTTT GAGAGAATGT ATAATCTTAG GTTGCTTGAA 
TTCCACAATC CATTCACAAG TGAATTGAAG ATATATCTTC CTAAAGGACT CCAGTTTCTT 
CCAGACGAGC TAAGATTTCT TCGTTGGGAC AAATACCCTT CAAAGTCTTT GCCAACAAGT 
TTTTGTGCTG AAAAGCTCAT GGAACTTCAC ATGCGTGAAA GCCAACTTAG AGAGCTTTGG 
AATGGAGTTC AGAATCTTGG AAATTTGGTA TCTGTTGACC TTAGCAACTC TAAGCATCTA 
GTTAGGATTC CAGACTTATG TAGAGCCCAA AACCTTGAGG TTTTAAAGTT GACTGGCTGT 
ACAAATTTAG TTGAGATTCC TTCGTCTATT CAAAATCTAA GCAAGCTAAC GCGGCTTATT 
CTAGATGAAT GCAAAAGTCT TGATAGTTTA TTGAGCTACC TTCCTCAAAA TATAAGAAAT 
CTGAGTTTAG ATGGAAGTAC AATAAAGCAA TTGCCCCCAT CAATTGGTGA GTTGAAATGT 
CTTGAAACAA TTGTTCTTCG AAGAAGCTCA GCACTGACAA TTCTCCCAGA CACTTTTTGC 
AATCTGAAAT CTCTTATAGA ACTTCATATA TCAGATTGTC CAAGTCTCAA TGAGCTGCCA 
CTGAACTTGG GGAATTTGGA ATCTTTGAAA GTACTGCATG CTAATAGAAG TGGTCTAAAA 
AACTTACCGT CCTCCGTGGA TCAATTGAGG CAATTGGAAT CATTGGATTG TTCAGGATGT 
AAAGATTTAA TGTTGCCTCC TTTTATAGAT ATGCCCCATT TGGAGGTTCT TTATCTAAAT 
GGCTGTGGTA TGTCAGAATT TCCTCAAGGT ATTTGCTCTC TGAAGTCATT GACCATCTTA 
GATGTGAGTG GAAATAATTT TGAGAGTATA CCTGCAAGCA TCAAACAACT TTCTAATTTG 
GAGAGGCTTC TTTTAAGAGA TTGCAAGAGA CTTCAATATT TACCGGATCT TCCATTGGCT 
CTACGATGTT TATATGCAAG AAACTGCACA TCTTTGGAAT CAGCGTCAAC TTCATTTTTA 
ATGGCACAAG AACCAAATTA TCAAACCATT TTAGATTTCT ATAAATGCAT GAAACTAAAT 
CAGGGCACAT ATAGTAGAAT TATGGACGAC ATATTGGCAA CACATTTGGT CAATGACAGA 
CAGTCTCGCT ACATAGGTGC CAAGATGTAT ATTGCTGGAA GTGAAATGGC AGAGAATATA 
AGGTATCACA CTAACAATGG ATCATCTCTG TCTTTCACAA TCGACCAGCA GCAGCCTGAC 
CTGATTGGTT TCTCTTTATG TGCTGTGGTT GCATCCAATG GTCATCCTCC TCCTCATGAC 
GTCATAAATA TTAGATGTAG AGCCCTTCTC ACAGGCGAGT CTGGACATAG CCATATTGAA 
GATTTGTACT TTTTTAGTTA TCCAGATGAA CTAGACATCC AATCGGAGAC TCTGTTTCTG 
TGGGCTAATT CAATCTCTTG GGACTCCGAA GACGGTTTGA GCAAGGCTTC ATTTCATTTC 
TTCATGGATT ATCATAATGA AGAAGACGAA GACTATTATG AGTGCAAGGA GGTGATAGTT 
AGGTGTGGAG TTCATCCAAT TTTCAAAGAT AAGAAGAGCA AAAATAAAGA TGAAAATGAG 
GAATCTCAGC AACCTCCTCT GAAAAGATTG AAAGAGGAAC ATCTAAGCGC CGTCTCTTGG 
ATGACTACAG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012068500.1
CDS	208..3582
Translation	MASTSSTAVRRKCDVFLNFRGGDTCENFVSELCKTLNRNLIETFIIDDKFGGRGGEEEILRKIQEADISSVIFSEDYASSALCMDELLKIVECKETKGQTVIPVFYRVDPTHVQHLKGNFGFGFDKLTQELRNSLPKVESWRSALIKTAYLKGWDSHFTKPESKLIHEIVNDILKKLNQVFFNGSVLVGISSHIREVESLLSIESQGVRSIGIWGMGGIGKTTITEVLFSRISAQFECQCYVPNVREQLEKRTVAHIRAEILSKLLGMENSIVRMPYIFPNFIRNKLRKKKVLIVLDDVDNSEVVRDLVGDCHLYGSGSRIIVTSRDKQVLKNADIKEYIYEVEKLVDFQAILLFSLHAFKQDAPPEEFMKLSERAITYAQGIPLAIKILGSSLYCKSVNEWESELAKLENSPDRTIEEVLKISYDGLESSEQSIFLDIACFFKGEDRTRIQSILNGCGFFADIGISRLLDKSLITISSNNQLEMHDLLQQMGRNIVCLECIHQPGERSRLWIPQDVYLVLKREKGTGNIEGISLDKSKIRDIELSPTVFERMYNLRLLEFHNPFTSELKIYLPKGLQFLPDELRFLRWDKYPSKSLPTSFCAEKLMELHMRESQLRELWNGVQNLGNLVSVDLSNSKHLVRIPDLCRAQNLEVLKLTGCTNLVEIPSSIQNLSKLTRLILDECKSLDSLLSYLPQNIRNLSLDGSTIKQLPPSIGELKCLETIVLRRSSALTILPDTFCNLKSLIELHISDCPSLNELPLNLGNLESLKVLHANRSGLKNLPSSVDQLRQLESLDCSGCKDLMLPPFIDMPHLEVLYLNGCGMSEFPQGICSLKSLTILDVSGNNFESIPASIKQLSNLERLLLRDCKRLQYLPDLPLALRCLYARNCTSLESASTSFLMAQEPNYQTILDFYKCMKLNQGTYSRIMDDILATHLVNDRQSRYIGAKMYIAGSEMAENIRYHTNNGSSLSFTIDQQQPDLIGFSLCAVVASNGHPPPHDVINIRCRALLTGESGHSHIEDLYFFSYPDELDIQSETLFLWANSISWDSEDGLSKASFHFFMDYHNEEDEDYYECKEVIVRCGVHPIFKDKKSKNKDENEESQQPPLKRLKEEHLSAVSWMTTD

Target ORF information:

RefSeq Version	XM_012213110.2
Organism	Jatropha curcas
Definition	Jatropha curcas TMV resistance protein N (LOC105631109), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012213110.2

ORF Insert Sequence:

ATGGCATCTA CTTCGTCCAC TGCTGTTCGC AGAAAATGCG ATGTGTTCCT TAATTTTAGA 
GGTGGAGACA CTTGCGAGAA CTTTGTCAGT GAACTCTGCA AAACTTTAAA TCGAAATCTG 
ATCGAGACTT TCATCATCGA TGATAAATTT GGTGGCAGAG GAGGAGAAGA AGAGATTCTG 
CGAAAAATAC AAGAAGCAGA TATTTCTTCA GTCATTTTCT CGGAAGACTA TGCTTCTTCT 
GCTTTGTGTA TGGATGAGCT CCTAAAGATA GTTGAATGTA AAGAGACGAA AGGGCAAACT 
GTGATACCAG TTTTTTACCG AGTAGATCCA ACGCATGTTC AACACCTGAA AGGAAATTTT 
GGGTTTGGAT TTGACAAACT TACTCAAGAA TTGAGGAACT CCTTGCCCAA AGTGGAGAGT 
TGGAGAAGTG CTTTGATTAA GACTGCATAT CTCAAGGGAT GGGATTCACA CTTCACCAAG 
CCTGAATCCA AGCTAATTCA TGAAATTGTG AATGATATTT TGAAGAAGTT GAATCAAGTG 
TTCTTCAATG GTTCTGTCTT AGTTGGAATT AGTTCACACA TCAGGGAAGT AGAATCATTA 
TTGTCCATTG AATCACAAGG TGTGCGCTCA ATAGGAATAT GGGGAATGGG AGGCATAGGC 
AAAACAACTA TTACTGAAGT TCTGTTTAGT CGAATTTCTG CTCAGTTTGA ATGTCAATGC 
TATGTCCCAA ATGTGAGGGA ACAACTGGAA AAGCGTACGG TAGCTCATAT AAGAGCTGAA 
ATTCTTTCTA AATTACTGGG AATGGAAAAT AGTATAGTCC GCATGCCCTA TATATTTCCA 
AATTTCATTA GGAATAAGCT TAGGAAGAAA AAAGTTCTCA TTGTTCTTGA TGATGTGGAT 
AATTCTGAGG TAGTAAGGGA TTTAGTAGGA GACTGTCATT TGTATGGTTC GGGAAGTAGA 
ATAATTGTAA CGAGTAGAGA CAAACAAGTA CTTAAAAATG CTGATATCAA GGAGTATATT 
TATGAGGTTG AGAAATTGGT TGACTTTCAA GCTATTCTAC TATTTAGCTT GCATGCTTTC 
AAACAAGATG CTCCACCAGA AGAATTTATG AAGCTATCAG AAAGGGCAAT AACCTATGCT 
CAAGGGATTC CATTAGCTAT TAAAATTTTA GGGTCCAGTC TATATTGCAA AAGTGTAAAT 
GAATGGGAAA GTGAATTGGC AAAACTTGAA AATAGCCCAG ACAGGACAAT TGAGGAAGTT 
CTGAAAATAA GTTATGATGG ATTAGAAAGC AGTGAACAAA GCATATTTCT TGATATTGCA 
TGCTTCTTTA AGGGAGAAGA TAGAACTCGC ATTCAAAGTA TATTGAATGG TTGCGGATTC 
TTTGCAGATA TTGGAATAAG TCGTCTCTTA GATAAGTCTC TGATAACTAT TTCATCTAAC 
AATCAGTTGG AAATGCATGA CCTACTACAA CAAATGGGAA GGAATATTGT TTGCTTGGAA 
TGCATTCATC AACCTGGAGA ACGCAGTAGA TTGTGGATTC CACAGGATGT CTATCTTGTA 
TTGAAAAGAG AAAAGGGGAC TGGAAACATT GAAGGAATAT CATTAGACAA GTCCAAAATC 
AGAGACATAG AACTAAGCCC AACAGTCTTT GAGAGAATGT ATAATCTTAG GTTGCTTGAA 
TTCCACAATC CATTCACAAG TGAATTGAAG ATATATCTTC CTAAAGGACT CCAGTTTCTT 
CCAGACGAGC TAAGATTTCT TCGTTGGGAC AAATACCCTT CAAAGTCTTT GCCAACAAGT 
TTTTGTGCTG AAAAGCTCAT GGAACTTCAC ATGCGTGAAA GCCAACTTAG AGAGCTTTGG 
AATGGAGTTC AGAATCTTGG AAATTTGGTA TCTGTTGACC TTAGCAACTC TAAGCATCTA 
GTTAGGATTC CAGACTTATG TAGAGCCCAA AACCTTGAGG TTTTAAAGTT GACTGGCTGT 
ACAAATTTAG TTGAGATTCC TTCGTCTATT CAAAATCTAA GCAAGCTAAC GCGGCTTATT 
CTAGATGAAT GCAAAAGTCT TGATAGTTTA TTGAGCTACC TTCCTCAAAA TATAAGAAAT 
CTGAGTTTAG ATGGAAGTAC AATAAAGCAA TTGCCCCCAT CAATTGGTGA GTTGAAATGT 
CTTGAAACAA TTGTTCTTCG AAGAAGCTCA GCACTGACAA TTCTCCCAGA CACTTTTTGC 
AATCTGAAAT CTCTTATAGA ACTTCATATA TCAGATTGTC CAAGTCTCAA TGAGCTGCCA 
CTGAACTTGG GGAATTTGGA ATCTTTGAAA GTACTGCATG CTAATAGAAG TGGTCTAAAA 
AACTTACCGT CCTCCGTGGA TCAATTGAGG CAATTGGAAT CATTGGATTG TTCAGGATGT 
AAAGATTTAA TGTTGCCTCC TTTTATAGAT ATGCCCCATT TGGAGGTTCT TTATCTAAAT 
GGCTGTGGTA TGTCAGAATT TCCTCAAGGT ATTTGCTCTC TGAAGTCATT GACCATCTTA 
GATGTGAGTG GAAATAATTT TGAGAGTATA CCTGCAAGCA TCAAACAACT TTCTAATTTG 
GAGAGGCTTC TTTTAAGAGA TTGCAAGAGA CTTCAATATT TACCGGATCT TCCATTGGCT 
CTACGATGTT TATATGCAAG AAACTGCACA TCTTTGGAAT CAGCGTCAAC TTCATTTTTA 
ATGGCACAAG AACCAAATTA TCAAACCATT TTAGATTTCT ATAAATGCAT GAAACTAAAT 
CAGGGCACAT ATAGTAGAAT TATGGACGAC ATATTGGCAA CACATTTGGT CAATGACAGA 
CAGTCTCGCT ACATAGGTGC CAAGATGTAT ATTGCTGGAA GTGAAATGGC AGAGAATATA 
AGGTATCACA CTAACAATGG ATCATCTCTG TCTTTCACAA TCGACCAGCA GCAGCCTGAC 
CTGATTGGTT TCTCTTTATG TGCTGTGGTT GCATCCAATG GTCATCCTCC TCCTCATGAC 
GTCATAAATA TTAGATGTAG AGCCCTTCTC ACAGGCGAGT CTGGACATAG CCATATTGAA 
GATTTGTACT TTTTTAGTTA TCCAGATGAA CTAGACATCC AATCGGAGAC TCTGTTTCTG 
TGGGCTAATT CAATCTCTTG GGACTCCGAA GACGGTTTGA GCAAGGCTTC ATTTCATTTC 
TTCATGGATT ATCATAATGA AGAAGACGAA GACTATTATG AGTGCAAGGA GGTGATAGTT 
AGGTGTGGAG TTCATCCAAT TTTCAAAGAT AAGAAGAGCA AAAATAAAGA TGAAAATGAG 
GAATCTCAGC AACCTCCTCT GAAAAGATTG AAAGAGGAAC ATCTAAGCGC CGTCTCTTGG 
ATGACTACAG ATTAA