LOC109191349 cDNA ORF clone, Ipomoea nil(Japanese morning glory)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109191349
Entrez Gene ID	109191349
Full Name	putative E3 ubiquitin-protein ligase RF298
Gene Type	protein-coding
Organism	Ipomoea nil(Japanese morning glory)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019341981.1	XP_019197526.1	putative E3 ubiquitin-protein ligase RF298
XM_019341980.1	XP_019197525.1	putative E3 ubiquitin-protein ligase RF298

Pathways from BioSystems
Gene Ontology

The following LOC109191349 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109191349 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIp20838	XM_019341980.1 Latest version!	Ipomoea nil putative E3 ubiquitin-protein ligase RF298 (LOC109191349), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OIp20838	XM_019341981.1 Latest version!	Ipomoea nil putative E3 ubiquitin-protein ligase RF298 (LOC109191349), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109191349 ( XM_019341981.1 ) cDNA ORF clone, Ipomoea nil(Japanese morning glory) -> XP_019197526.1 Ipomoea nil putative E3 ubiquitin-protein ligase RF298 (LOC109191349), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OIp20838
Clone ID Related Accession (Same CDS sequence)	XM_019341981.1 , XM_019341980.1
Accession Version	XM_019341981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Ipomoea nil(Japanese morning glory)
Product	putative E3 ubiquitin-protein ligase RF298
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017618047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ipomoea nil Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAGCA AAAAAGAAAT GGATGGCAGT GATGATGGTA GCAATGAGAT GGTATCTTCT 
GTTTCAACCC AAGAGAACGG AAGTAGGAAT AAGAGGAAGT TTTTGTCCGA ATTCCCGTTA 
GACATTCCCG TAGATTCACC GGCTCCGTCT CTGACAGAAT TCCCGAGATA CGAGTTATTA 
GAGGAAAAGC TCCATAACAC GCTAAATGAG TTTGCATCAC TTGACCTGCA GTCTGGGCAG 
ACTAACGAGA AACGTGAAAT GGAAAGTTCA CAAGTAGCTG ATTGGGACGA TCCGGTTGCA 
TGTGAGCTCG AGAAACTTCT AATAAGTAAT CTTCATGCAG CTTTCCGGAG TGCAATCAAG 
AAGATAGTTG AATGTGGGTA CAGCCAGGAA CTCGCGGAAT TGGTTGTTTT GAGGAGTGGA 
CTTTATCATG GCGTTAAGGA TGTTGTCTCG AATGTTGTTG ATGGTGCGTT GGCTTTGTTA 
ACAAAAGAAA GGTATTTGGG TACTAGGTAC CAGGTATTCG AGGAGTTCGA TAGCCTAGTG 
GAGTACGCGA TCCTCGAGAT GATCTGTGTG CTGAGGGAAG TTAAGCCGTT TTTAACCGTT 
GCTGAAGCGA TGTGGTACCT CTTGATTTGC GATCTGAACC TGTTACACGC ATGTGCAGTG 
AAGGGAGATC CATCGTGCGA GTTCTTTGGT CAAGTAAATC ACGGGGAGAG CTCGTCTGAT 
ACAAAGGCTC CCCAGTCGAA AACCGAAGTT TCTAAAGCCA GTTCAAACAA TCAAAAATCA 
CATCTTTTGA AATCTTCCGT TACTAGCTCT CAAAACCCGC AGTCTAAAGC TGTTGCCGCG 
TCTCAACTAC CGAAAAACTC GCGATTTCGC GAAGTTGCGA TGACTGGGAA AGAAGGCTCG 
GTGTCCCTTC TGGAGATGGA GGGGAAGTCT TTAGGTGCCG AAGAAAAATC CGGCGGGGCT 
GGTCGAAAGG GATCGTCACT TCTTTCCAAG AAAGATCTCC TACGGCAAAA GGCGTTTCAT 
TTCGAGAAGA CCTACAAAGG TCGTGTGGGT AAGGGGGGGC CCTCCTTTAA GGCCAAATTT 
ACTTCCTGGG GCAGCATGGT TCTCGACAAA ACCCTAAATC CTCCTCAACC CGGTTCTTCT 
AGTACAGCAA CGAAACTCAC TTCCTCTAAG ATAATCCCAT CTCCAAAGCC CCCGGGCAAC 
ACCTCAACCT CTTCCAGTTT GTCAAGTTAT TATACCGGTA TCCCTTACGA CGAATCTCAG 
GGGAAATACG TTGCTCAAAA CGAGAAAGAT GAAAAGATAT TAACGCTGAT TTCCTGCGTT 
CACAAGCTAG AGAAAGAAAT CACCGATTTG TCAGATTGTG CAGCCGAGAA GGTTAGGATG 
GTTACTCGAA GGCTGAGCAA GGATCAAGCC GAGTTGAGAA CGTTGAGACA AGAAAAAGAA 
GAGGTCGAAA AATTTCTCAA GGACAAGCAA ACACTGGAGG AGAACACTTT GAAGAGGCTC 
ACTGAAATGG AGTACGCAAT ATCCAACGCT AGTGCCCAGA TGGAGATGGC TAACTCCTCG 
CTTCATAGGC TCGAAGCGGA ACATGCCGTG CTTCAAAAGA AGATGGAGGC TGCAAAATCG 
CATTCTCTCG AGTCGTCTCG AAAATCCCAG GAAGCCATGG TGAGAGAGCA GGAGGCACTG 
AAGAAGTGTC AAGGTGGGGA GGCCGAGAAG AGCTTATTGC AGGAAAAATT CTCAGCTCTG 
AAATGCGAAA GAGCTAACTT GGAGCAGCAA TTAGAGAAGG CTAAGAAACG GCTAAAACAG 
ATCGAGGCTC TTGCAGAACA GGAAAAGAAG GAGAAAGAAG AACATGTTAG GCAGGCCAAA 
TTCCTGCAGG GTAAACGAGA GCGACTGGGA GCTTTGGCGA AAATAGAGGA GGATAATATT 
AGAGAAAATG CAGAAAAAGA GATGCAGAAG TGTAAAAATG AAATCGACAA GCTTGCAAGT 
GAAATCTCTG TACGAATATT AGCGTCTGAA GCTTCAAAAA TAGAGGCACT CCGGAGGGGC 
ATTGGCTCGG GCTATGGTAA GTTGGGGCCT ATGATGAGGG AGGGAGGTGA AAAGCGAGAA 
CGAGAGTGCG TTATGTGTTT GACAGACGAA ATTTCGGTGG TTTTCCTACC GTGTGCACAT 
CAAGTTCTTT GCACGCAGTG CAACATTCGT CACGAGAAGC AAGGAATGAA CGACTGCCCT 
TCTTGTCGAA CGCCAATCAA GACAAGGATC AATGTGCGCT ATTCTCCTTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019197526.1
CDS	217..2490
Translation	MVSKKEMDGSDDGSNEMVSSVSTQENGSRNKRKFLSEFPLDIPVDSPAPSLTEFPRYELLEEKLHNTLNEFASLDLQSGQTNEKREMESSQVADWDDPVACELEKLLISNLHAAFRSAIKKIVECGYSQELAELVVLRSGLYHGVKDVVSNVVDGALALLTKERYLGTRYQVFEEFDSLVEYAILEMICVLREVKPFLTVAEAMWYLLICDLNLLHACAVKGDPSCEFFGQVNHGESSSDTKAPQSKTEVSKASSNNQKSHLLKSSVTSSQNPQSKAVAASQLPKNSRFREVAMTGKEGSVSLLEMEGKSLGAEEKSGGAGRKGSSLLSKKDLLRQKAFHFEKTYKGRVGKGGPSFKAKFTSWGSMVLDKTLNPPQPGSSSTATKLTSSKIIPSPKPPGNTSTSSSLSSYYTGIPYDESQGKYVAQNEKDEKILTLISCVHKLEKEITDLSDCAAEKVRMVTRRLSKDQAELRTLRQEKEEVEKFLKDKQTLEENTLKRLTEMEYAISNASAQMEMANSSLHRLEAEHAVLQKKMEAAKSHSLESSRKSQEAMVREQEALKKCQGGEAEKSLLQEKFSALKCERANLEQQLEKAKKRLKQIEALAEQEKKEKEEHVRQAKFLQGKRERLGALAKIEEDNIRENAEKEMQKCKNEIDKLASEISVRILASEASKIEALRRGIGSGYGKLGPMMREGGEKRERECVMCLTDEISVVFLPCAHQVLCTQCNIRHEKQGMNDCPSCRTPIKTRINVRYSPS

Target ORF information:

RefSeq Version	XM_019341981.1
Organism	Ipomoea nil(Japanese morning glory)
Definition	Ipomoea nil putative E3 ubiquitin-protein ligase RF298 (LOC109191349), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019341981.1

ORF Insert Sequence:

ATGGTTAGCA AAAAAGAAAT GGATGGCAGT GATGATGGTA GCAATGAGAT GGTATCTTCT 
GTTTCAACCC AAGAGAACGG AAGTAGGAAT AAGAGGAAGT TTTTGTCCGA ATTCCCGTTA 
GACATTCCCG TAGATTCACC GGCTCCGTCT CTGACAGAAT TCCCGAGATA CGAGTTATTA 
GAGGAAAAGC TCCATAACAC GCTAAATGAG TTTGCATCAC TTGACCTGCA GTCTGGGCAG 
ACTAACGAGA AACGTGAAAT GGAAAGTTCA CAAGTAGCTG ATTGGGACGA TCCGGTTGCA 
TGTGAGCTCG AGAAACTTCT AATAAGTAAT CTTCATGCAG CTTTCCGGAG TGCAATCAAG 
AAGATAGTTG AATGTGGGTA CAGCCAGGAA CTCGCGGAAT TGGTTGTTTT GAGGAGTGGA 
CTTTATCATG GCGTTAAGGA TGTTGTCTCG AATGTTGTTG ATGGTGCGTT GGCTTTGTTA 
ACAAAAGAAA GGTATTTGGG TACTAGGTAC CAGGTATTCG AGGAGTTCGA TAGCCTAGTG 
GAGTACGCGA TCCTCGAGAT GATCTGTGTG CTGAGGGAAG TTAAGCCGTT TTTAACCGTT 
GCTGAAGCGA TGTGGTACCT CTTGATTTGC GATCTGAACC TGTTACACGC ATGTGCAGTG 
AAGGGAGATC CATCGTGCGA GTTCTTTGGT CAAGTAAATC ACGGGGAGAG CTCGTCTGAT 
ACAAAGGCTC CCCAGTCGAA AACCGAAGTT TCTAAAGCCA GTTCAAACAA TCAAAAATCA 
CATCTTTTGA AATCTTCCGT TACTAGCTCT CAAAACCCGC AGTCTAAAGC TGTTGCCGCG 
TCTCAACTAC CGAAAAACTC GCGATTTCGC GAAGTTGCGA TGACTGGGAA AGAAGGCTCG 
GTGTCCCTTC TGGAGATGGA GGGGAAGTCT TTAGGTGCCG AAGAAAAATC CGGCGGGGCT 
GGTCGAAAGG GATCGTCACT TCTTTCCAAG AAAGATCTCC TACGGCAAAA GGCGTTTCAT 
TTCGAGAAGA CCTACAAAGG TCGTGTGGGT AAGGGGGGGC CCTCCTTTAA GGCCAAATTT 
ACTTCCTGGG GCAGCATGGT TCTCGACAAA ACCCTAAATC CTCCTCAACC CGGTTCTTCT 
AGTACAGCAA CGAAACTCAC TTCCTCTAAG ATAATCCCAT CTCCAAAGCC CCCGGGCAAC 
ACCTCAACCT CTTCCAGTTT GTCAAGTTAT TATACCGGTA TCCCTTACGA CGAATCTCAG 
GGGAAATACG TTGCTCAAAA CGAGAAAGAT GAAAAGATAT TAACGCTGAT TTCCTGCGTT 
CACAAGCTAG AGAAAGAAAT CACCGATTTG TCAGATTGTG CAGCCGAGAA GGTTAGGATG 
GTTACTCGAA GGCTGAGCAA GGATCAAGCC GAGTTGAGAA CGTTGAGACA AGAAAAAGAA 
GAGGTCGAAA AATTTCTCAA GGACAAGCAA ACACTGGAGG AGAACACTTT GAAGAGGCTC 
ACTGAAATGG AGTACGCAAT ATCCAACGCT AGTGCCCAGA TGGAGATGGC TAACTCCTCG 
CTTCATAGGC TCGAAGCGGA ACATGCCGTG CTTCAAAAGA AGATGGAGGC TGCAAAATCG 
CATTCTCTCG AGTCGTCTCG AAAATCCCAG GAAGCCATGG TGAGAGAGCA GGAGGCACTG 
AAGAAGTGTC AAGGTGGGGA GGCCGAGAAG AGCTTATTGC AGGAAAAATT CTCAGCTCTG 
AAATGCGAAA GAGCTAACTT GGAGCAGCAA TTAGAGAAGG CTAAGAAACG GCTAAAACAG 
ATCGAGGCTC TTGCAGAACA GGAAAAGAAG GAGAAAGAAG AACATGTTAG GCAGGCCAAA 
TTCCTGCAGG GTAAACGAGA GCGACTGGGA GCTTTGGCGA AAATAGAGGA GGATAATATT 
AGAGAAAATG CAGAAAAAGA GATGCAGAAG TGTAAAAATG AAATCGACAA GCTTGCAAGT 
GAAATCTCTG TACGAATATT AGCGTCTGAA GCTTCAAAAA TAGAGGCACT CCGGAGGGGC 
ATTGGCTCGG GCTATGGTAA GTTGGGGCCT ATGATGAGGG AGGGAGGTGA AAAGCGAGAA 
CGAGAGTGCG TTATGTGTTT GACAGACGAA ATTTCGGTGG TTTTCCTACC GTGTGCACAT 
CAAGTTCTTT GCACGCAGTG CAACATTCGT CACGAGAAGC AAGGAATGAA CGACTGCCCT 
TCTTGTCGAA CGCCAATCAA GACAAGGATC AATGTGCGCT ATTCTCCTTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109191349 ( XM_019341980.1 ) cDNA ORF clone, Ipomoea nil(Japanese morning glory) -> XP_019197525.1 Ipomoea nil putative E3 ubiquitin-protein ligase RF298 (LOC109191349), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIp20838
Clone ID Related Accession (Same CDS sequence)	XM_019341981.1 , XM_019341980.1
Accession Version	XM_019341980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Ipomoea nil(Japanese morning glory)
Product	putative E3 ubiquitin-protein ligase RF298
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017618047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ipomoea nil Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAGCA AAAAAGAAAT GGATGGCAGT GATGATGGTA GCAATGAGAT GGTATCTTCT 
GTTTCAACCC AAGAGAACGG AAGTAGGAAT AAGAGGAAGT TTTTGTCCGA ATTCCCGTTA 
GACATTCCCG TAGATTCACC GGCTCCGTCT CTGACAGAAT TCCCGAGATA CGAGTTATTA 
GAGGAAAAGC TCCATAACAC GCTAAATGAG TTTGCATCAC TTGACCTGCA GTCTGGGCAG 
ACTAACGAGA AACGTGAAAT GGAAAGTTCA CAAGTAGCTG ATTGGGACGA TCCGGTTGCA 
TGTGAGCTCG AGAAACTTCT AATAAGTAAT CTTCATGCAG CTTTCCGGAG TGCAATCAAG 
AAGATAGTTG AATGTGGGTA CAGCCAGGAA CTCGCGGAAT TGGTTGTTTT GAGGAGTGGA 
CTTTATCATG GCGTTAAGGA TGTTGTCTCG AATGTTGTTG ATGGTGCGTT GGCTTTGTTA 
ACAAAAGAAA GGTATTTGGG TACTAGGTAC CAGGTATTCG AGGAGTTCGA TAGCCTAGTG 
GAGTACGCGA TCCTCGAGAT GATCTGTGTG CTGAGGGAAG TTAAGCCGTT TTTAACCGTT 
GCTGAAGCGA TGTGGTACCT CTTGATTTGC GATCTGAACC TGTTACACGC ATGTGCAGTG 
AAGGGAGATC CATCGTGCGA GTTCTTTGGT CAAGTAAATC ACGGGGAGAG CTCGTCTGAT 
ACAAAGGCTC CCCAGTCGAA AACCGAAGTT TCTAAAGCCA GTTCAAACAA TCAAAAATCA 
CATCTTTTGA AATCTTCCGT TACTAGCTCT CAAAACCCGC AGTCTAAAGC TGTTGCCGCG 
TCTCAACTAC CGAAAAACTC GCGATTTCGC GAAGTTGCGA TGACTGGGAA AGAAGGCTCG 
GTGTCCCTTC TGGAGATGGA GGGGAAGTCT TTAGGTGCCG AAGAAAAATC CGGCGGGGCT 
GGTCGAAAGG GATCGTCACT TCTTTCCAAG AAAGATCTCC TACGGCAAAA GGCGTTTCAT 
TTCGAGAAGA CCTACAAAGG TCGTGTGGGT AAGGGGGGGC CCTCCTTTAA GGCCAAATTT 
ACTTCCTGGG GCAGCATGGT TCTCGACAAA ACCCTAAATC CTCCTCAACC CGGTTCTTCT 
AGTACAGCAA CGAAACTCAC TTCCTCTAAG ATAATCCCAT CTCCAAAGCC CCCGGGCAAC 
ACCTCAACCT CTTCCAGTTT GTCAAGTTAT TATACCGGTA TCCCTTACGA CGAATCTCAG 
GGGAAATACG TTGCTCAAAA CGAGAAAGAT GAAAAGATAT TAACGCTGAT TTCCTGCGTT 
CACAAGCTAG AGAAAGAAAT CACCGATTTG TCAGATTGTG CAGCCGAGAA GGTTAGGATG 
GTTACTCGAA GGCTGAGCAA GGATCAAGCC GAGTTGAGAA CGTTGAGACA AGAAAAAGAA 
GAGGTCGAAA AATTTCTCAA GGACAAGCAA ACACTGGAGG AGAACACTTT GAAGAGGCTC 
ACTGAAATGG AGTACGCAAT ATCCAACGCT AGTGCCCAGA TGGAGATGGC TAACTCCTCG 
CTTCATAGGC TCGAAGCGGA ACATGCCGTG CTTCAAAAGA AGATGGAGGC TGCAAAATCG 
CATTCTCTCG AGTCGTCTCG AAAATCCCAG GAAGCCATGG TGAGAGAGCA GGAGGCACTG 
AAGAAGTGTC AAGGTGGGGA GGCCGAGAAG AGCTTATTGC AGGAAAAATT CTCAGCTCTG 
AAATGCGAAA GAGCTAACTT GGAGCAGCAA TTAGAGAAGG CTAAGAAACG GCTAAAACAG 
ATCGAGGCTC TTGCAGAACA GGAAAAGAAG GAGAAAGAAG AACATGTTAG GCAGGCCAAA 
TTCCTGCAGG GTAAACGAGA GCGACTGGGA GCTTTGGCGA AAATAGAGGA GGATAATATT 
AGAGAAAATG CAGAAAAAGA GATGCAGAAG TGTAAAAATG AAATCGACAA GCTTGCAAGT 
GAAATCTCTG TACGAATATT AGCGTCTGAA GCTTCAAAAA TAGAGGCACT CCGGAGGGGC 
ATTGGCTCGG GCTATGGTAA GTTGGGGCCT ATGATGAGGG AGGGAGGTGA AAAGCGAGAA 
CGAGAGTGCG TTATGTGTTT GACAGACGAA ATTTCGGTGG TTTTCCTACC GTGTGCACAT 
CAAGTTCTTT GCACGCAGTG CAACATTCGT CACGAGAAGC AAGGAATGAA CGACTGCCCT 
TCTTGTCGAA CGCCAATCAA GACAAGGATC AATGTGCGCT ATTCTCCTTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019197525.1
CDS	202..2475
Translation	MVSKKEMDGSDDGSNEMVSSVSTQENGSRNKRKFLSEFPLDIPVDSPAPSLTEFPRYELLEEKLHNTLNEFASLDLQSGQTNEKREMESSQVADWDDPVACELEKLLISNLHAAFRSAIKKIVECGYSQELAELVVLRSGLYHGVKDVVSNVVDGALALLTKERYLGTRYQVFEEFDSLVEYAILEMICVLREVKPFLTVAEAMWYLLICDLNLLHACAVKGDPSCEFFGQVNHGESSSDTKAPQSKTEVSKASSNNQKSHLLKSSVTSSQNPQSKAVAASQLPKNSRFREVAMTGKEGSVSLLEMEGKSLGAEEKSGGAGRKGSSLLSKKDLLRQKAFHFEKTYKGRVGKGGPSFKAKFTSWGSMVLDKTLNPPQPGSSSTATKLTSSKIIPSPKPPGNTSTSSSLSSYYTGIPYDESQGKYVAQNEKDEKILTLISCVHKLEKEITDLSDCAAEKVRMVTRRLSKDQAELRTLRQEKEEVEKFLKDKQTLEENTLKRLTEMEYAISNASAQMEMANSSLHRLEAEHAVLQKKMEAAKSHSLESSRKSQEAMVREQEALKKCQGGEAEKSLLQEKFSALKCERANLEQQLEKAKKRLKQIEALAEQEKKEKEEHVRQAKFLQGKRERLGALAKIEEDNIRENAEKEMQKCKNEIDKLASEISVRILASEASKIEALRRGIGSGYGKLGPMMREGGEKRERECVMCLTDEISVVFLPCAHQVLCTQCNIRHEKQGMNDCPSCRTPIKTRINVRYSPS

Target ORF information:

RefSeq Version	XM_019341980.1
Organism	Ipomoea nil(Japanese morning glory)
Definition	Ipomoea nil putative E3 ubiquitin-protein ligase RF298 (LOC109191349), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019341980.1

ORF Insert Sequence:

ATGGTTAGCA AAAAAGAAAT GGATGGCAGT GATGATGGTA GCAATGAGAT GGTATCTTCT 
GTTTCAACCC AAGAGAACGG AAGTAGGAAT AAGAGGAAGT TTTTGTCCGA ATTCCCGTTA 
GACATTCCCG TAGATTCACC GGCTCCGTCT CTGACAGAAT TCCCGAGATA CGAGTTATTA 
GAGGAAAAGC TCCATAACAC GCTAAATGAG TTTGCATCAC TTGACCTGCA GTCTGGGCAG 
ACTAACGAGA AACGTGAAAT GGAAAGTTCA CAAGTAGCTG ATTGGGACGA TCCGGTTGCA 
TGTGAGCTCG AGAAACTTCT AATAAGTAAT CTTCATGCAG CTTTCCGGAG TGCAATCAAG 
AAGATAGTTG AATGTGGGTA CAGCCAGGAA CTCGCGGAAT TGGTTGTTTT GAGGAGTGGA 
CTTTATCATG GCGTTAAGGA TGTTGTCTCG AATGTTGTTG ATGGTGCGTT GGCTTTGTTA 
ACAAAAGAAA GGTATTTGGG TACTAGGTAC CAGGTATTCG AGGAGTTCGA TAGCCTAGTG 
GAGTACGCGA TCCTCGAGAT GATCTGTGTG CTGAGGGAAG TTAAGCCGTT TTTAACCGTT 
GCTGAAGCGA TGTGGTACCT CTTGATTTGC GATCTGAACC TGTTACACGC ATGTGCAGTG 
AAGGGAGATC CATCGTGCGA GTTCTTTGGT CAAGTAAATC ACGGGGAGAG CTCGTCTGAT 
ACAAAGGCTC CCCAGTCGAA AACCGAAGTT TCTAAAGCCA GTTCAAACAA TCAAAAATCA 
CATCTTTTGA AATCTTCCGT TACTAGCTCT CAAAACCCGC AGTCTAAAGC TGTTGCCGCG 
TCTCAACTAC CGAAAAACTC GCGATTTCGC GAAGTTGCGA TGACTGGGAA AGAAGGCTCG 
GTGTCCCTTC TGGAGATGGA GGGGAAGTCT TTAGGTGCCG AAGAAAAATC CGGCGGGGCT 
GGTCGAAAGG GATCGTCACT TCTTTCCAAG AAAGATCTCC TACGGCAAAA GGCGTTTCAT 
TTCGAGAAGA CCTACAAAGG TCGTGTGGGT AAGGGGGGGC CCTCCTTTAA GGCCAAATTT 
ACTTCCTGGG GCAGCATGGT TCTCGACAAA ACCCTAAATC CTCCTCAACC CGGTTCTTCT 
AGTACAGCAA CGAAACTCAC TTCCTCTAAG ATAATCCCAT CTCCAAAGCC CCCGGGCAAC 
ACCTCAACCT CTTCCAGTTT GTCAAGTTAT TATACCGGTA TCCCTTACGA CGAATCTCAG 
GGGAAATACG TTGCTCAAAA CGAGAAAGAT GAAAAGATAT TAACGCTGAT TTCCTGCGTT 
CACAAGCTAG AGAAAGAAAT CACCGATTTG TCAGATTGTG CAGCCGAGAA GGTTAGGATG 
GTTACTCGAA GGCTGAGCAA GGATCAAGCC GAGTTGAGAA CGTTGAGACA AGAAAAAGAA 
GAGGTCGAAA AATTTCTCAA GGACAAGCAA ACACTGGAGG AGAACACTTT GAAGAGGCTC 
ACTGAAATGG AGTACGCAAT ATCCAACGCT AGTGCCCAGA TGGAGATGGC TAACTCCTCG 
CTTCATAGGC TCGAAGCGGA ACATGCCGTG CTTCAAAAGA AGATGGAGGC TGCAAAATCG 
CATTCTCTCG AGTCGTCTCG AAAATCCCAG GAAGCCATGG TGAGAGAGCA GGAGGCACTG 
AAGAAGTGTC AAGGTGGGGA GGCCGAGAAG AGCTTATTGC AGGAAAAATT CTCAGCTCTG 
AAATGCGAAA GAGCTAACTT GGAGCAGCAA TTAGAGAAGG CTAAGAAACG GCTAAAACAG 
ATCGAGGCTC TTGCAGAACA GGAAAAGAAG GAGAAAGAAG AACATGTTAG GCAGGCCAAA 
TTCCTGCAGG GTAAACGAGA GCGACTGGGA GCTTTGGCGA AAATAGAGGA GGATAATATT 
AGAGAAAATG CAGAAAAAGA GATGCAGAAG TGTAAAAATG AAATCGACAA GCTTGCAAGT 
GAAATCTCTG TACGAATATT AGCGTCTGAA GCTTCAAAAA TAGAGGCACT CCGGAGGGGC 
ATTGGCTCGG GCTATGGTAA GTTGGGGCCT ATGATGAGGG AGGGAGGTGA AAAGCGAGAA 
CGAGAGTGCG TTATGTGTTT GACAGACGAA ATTTCGGTGG TTTTCCTACC GTGTGCACAT 
CAAGTTCTTT GCACGCAGTG CAACATTCGT CACGAGAAGC AAGGAATGAA CGACTGCCCT 
TCTTGTCGAA CGCCAATCAA GACAAGGATC AATGTGCGCT ATTCTCCTTC CTAG