Itch cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itch
Entrez Gene ID	101977844
Full Name	itchy E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021729578.1	XP_021585253.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
XM_005329914.2	XP_005329971.1	E3 ubiquitin-protein ligase Itchy homolog
XM_021729577.1	XP_021585252.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
XM_005329914.3	XP_005329971.1	E3 ubiquitin-protein ligase Itchy homolog isoform X2
XM_021729576.1	XP_021585251.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1

Pathways from BioSystems
Gene Ontology

The following Itch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIc60905	XM_005329914.3 Latest version!	Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OIc80270	XM_021729576.1 Latest version!	Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OIc80270	XM_021729578.1 Latest version!	Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OIc80270	XM_021729577.1 Latest version!	Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OIc60905	XM_005329914.2	Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itch ( XM_005329914.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005329971.1 Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), mRNA.

Price & Availability↑

CloneID	OIc60905
Clone ID Related Accession (Same CDS sequence)	XM_005329914.2 , XM_005329914.3
Accession Version	XM_005329914.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-11
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	E3 ubiquitin-protein ligase Itchy homolog
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936561.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 12, 2015 this sequence version replaced XM_005329914.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGTAGCTT GATCATGAAG TCACAACTTC AGGTCACTGT CATTTCAGCA 
AAACTTAAAG AAAATAAAAA GAATTGGTTT GGACCAAGTC CTTATGTGGA AGTCACAGTG 
GATGGACAGT CAAAAAAGAC AGAAAAATGC AACAATACCA ACAGCCCCAA ATGGAAGCAA 
CCCCTTACAG TTATCGTTAC CCCTGTGAGT AAATTACATT TTCGAGTGTG GAGTCACCAG 
ACACTGAAGT CTGATGTTTT ATTGGGAACT GCTGCATTAG ATATTTATGA AACATTAAAG 
TCAAACAATA TGAAACTTGA AGAAGTGATT GTGACTTTGC AGCTTGCAGG TGACAAAGAG 
CCAACAGAGA CAATGGGAGA CTTGTCAATT TGTCTTGATG GGCTGCAGTT AGAAGCTGAA 
GTTGTTACTA ATGGTGAAAC TACATGTTCA GAAAGTACTT CACAGAATGA TGATGGCTCC 
AGATCCAAGG ATGAAACAAG AGTGAACACA AGTGGTTCAG ATGACCCTGA GGATGGAGGG 
TCTGGTGAAA ATAGAAGGGT CAATGGGAAC AATTCTCCAT CACTGTCAAA TGGTGGTTTT 
AAACCTTCTA GACCTCCAAG ACCTTCACGA CCACCTCCAC CAACCCCACG TAGACCAGCA 
TCTGTCAATG GTTCACCATC TACCACTTCT GAAAGTGATG GATCTAGTAC AGGTTCTCTG 
CCACCAACAA ATACAAATAC AAATACATCT GAAGGAGCAA CATCTGGATT AATAATTCCC 
CTTACTATAT CTGGAGGCTC AGGTCCTAGG CCATTGAATC CTGTAACTCA AGCTCCCCTA 
CCACCTGGTT GGGAGCAAAG AGTGAACCAG CATGGGCGAG TTTATTATGT AGATCATGTT 
GAGAAAAGAA CAACGTGGGA TAGACCTGAG CCTCTACCTC CTGGCTGGGA ACGTCGAGTT 
GACAATATGG GACGCATTTA TTATGTTGAC CATTTCACAA GAACAACAAC ATGGCAGAGG 
CCAACATTGG AATCTGTCCG GAACTACGAA CAGTGGCAGC TACAACGTAG TCAACTTCAA 
GGAGCAATGC AGCAGTTTAA CCAGAGATTC ATTTATGGAA ATCAAGATTT GTTTGCTACA 
TCACAAAATA AAGAATTTGA TCCCCTTGGT CCTTTGCCAC CGGGATGGGA GAAGAGAACT 
GATAGCAATG GCAGAGTATA TTTTGTCAAC CACAACACTC GAATTACACA ATGGGAAGAC 
CCCAGAAATC AAGGTCAGTT AAATGAAAAG CCGTTACCTG AAGGCTGGGA AATGAGATTC 
ACAGTGGATG GAATTCCATA TTTTGTAGAC CACAATAAAA GAACAACTAC CTATATAGAT 
CCACGCACAG GAAAATCTGC CCTAGACAAT GGACCTCAGA TAGCCTATGT TCGGGACTTC 
AAGGCAAAGG TTCAGTATTT CCGGTTCTGG TGCCAGCAAT TGGCCATGCC ACAGCACATA 
AAGATTACAG TAACAAGAAA AACACTGTTT GAGGATTCTT TTCAACAGAT AATGAACTTC 
AGTCCCCAAG ATCTGCGAAG ACGATTGTGG GTGATTTTTC CAGGAGAAGA AGGTTTAGAT 
TATGGAGGTG TAGCAAGAGA GTGGTTCTTT CTTTTGTCAC ATGAAGTTTT GAACCCAATG 
TATTGCCTGT TTGAATATGC AGGGAAGGAT AACTATTGCC TGCAGATAAA CCCCGCTTCT 
TACATCAATC CAGATCACCT GAAATATTTT CGTTTTATTG GCAGGTTTAT TGCCATGGCT 
CTGTTCCATG GAAAATTCAT AGACACAGGT TTTTCTTTAC CATTCTATAA ATGTATCTTG 
AACAAACCAG TTGGACTTAA GGATTTAGAA TCTATTGATC CAGAATTTTA CAATTCTCTC 
ATCTGGGTTA AAGAAAACAA TATTGAAGAA TGTGGTTTGG AAATGTACTT CTCCGTTGAT 
AAAGAAATTC TAGGTGAAAT TAAAAGTCAT GACTTGAAAC CCAATGGTGG CAATATTCTT 
GTAACAGAAG AAAATAAAGA GGAATATATC AGAATGGTAG CTGAGTGGCG GTTATCTCGA 
GGTGTTGAAG AACAGACACA AGCTTTCTTT GAGGGTTTTA ATGAAATTCT TCCTCAGCAG 
TATTTGCAAT ACTTTGATGC AAAGGAATTA GAGGTCCTTT TATGTGGAAT GCAAGAGATT 
GATTTAAATG ACTGGCAAAG ACATGCAATC TACCGTCATT ACACTAGGAC AAGCAAACAA 
ATCATGTGGT TTTGGCAGTT TGTTAAGGAA ATTGATAATG AGAAGAGAAT GAGACTTCTG 
CAATTTGTCA CTGGAACTTG CCGATTACCA GTAGGAGGAT TTGCTGACCT CATGGGAAGC 
AATGGACCAC AGAAATTCTG CATTGAGAAA GTTGGGAAAG AAAATTGGTT ACCCAGAAGT 
CATACCTGTT TTAACCGCCT GGACCTTCCA CCCTACAAAA GCTACGAGCA ACTAAAGGAA 
AAGCTGTTGT TTGCCATTGA AGAAACAGAA GGATTTGGAC AAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005329971.1
CDS	170..2737
Translation	MSDSGSLIMKSQLQVTVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVIVTLQLAGDKEPTETMGDLSICLDGLQLEAEVVTNGETTCSESTSQNDDGSRSKDETRVNTSGSDDPEDGGSGENRRVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTTSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVNQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRNQGQLNEKPLPEGWEMRFTVDGIPYFVDHNKRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMNFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKCILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_005329914.2
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005329914.2

ORF Insert Sequence:

ATGTCTGACA GTGGTAGCTT GATCATGAAG TCACAACTTC AGGTCACTGT CATTTCAGCA 
AAACTTAAAG AAAATAAAAA GAATTGGTTT GGACCAAGTC CTTATGTGGA AGTCACAGTG 
GATGGACAGT CAAAAAAGAC AGAAAAATGC AACAATACCA ACAGCCCCAA ATGGAAGCAA 
CCCCTTACAG TTATCGTTAC CCCTGTGAGT AAATTACATT TTCGAGTGTG GAGTCACCAG 
ACACTGAAGT CTGATGTTTT ATTGGGAACT GCTGCATTAG ATATTTATGA AACATTAAAG 
TCAAACAATA TGAAACTTGA AGAAGTGATT GTGACTTTGC AGCTTGCAGG TGACAAAGAG 
CCAACAGAGA CAATGGGAGA CTTGTCAATT TGTCTTGATG GGCTGCAGTT AGAAGCTGAA 
GTTGTTACTA ATGGTGAAAC TACATGTTCA GAAAGTACTT CACAGAATGA TGATGGCTCC 
AGATCCAAGG ATGAAACAAG AGTGAACACA AGTGGTTCAG ATGACCCTGA GGATGGAGGG 
TCTGGTGAAA ATAGAAGGGT CAATGGGAAC AATTCTCCAT CACTGTCAAA TGGTGGTTTT 
AAACCTTCTA GACCTCCAAG ACCTTCACGA CCACCTCCAC CAACCCCACG TAGACCAGCA 
TCTGTCAATG GTTCACCATC TACCACTTCT GAAAGTGATG GATCTAGTAC AGGTTCTCTG 
CCACCAACAA ATACAAATAC AAATACATCT GAAGGAGCAA CATCTGGATT AATAATTCCC 
CTTACTATAT CTGGAGGCTC AGGTCCTAGG CCATTGAATC CTGTAACTCA AGCTCCCCTA 
CCACCTGGTT GGGAGCAAAG AGTGAACCAG CATGGGCGAG TTTATTATGT AGATCATGTT 
GAGAAAAGAA CAACGTGGGA TAGACCTGAG CCTCTACCTC CTGGCTGGGA ACGTCGAGTT 
GACAATATGG GACGCATTTA TTATGTTGAC CATTTCACAA GAACAACAAC ATGGCAGAGG 
CCAACATTGG AATCTGTCCG GAACTACGAA CAGTGGCAGC TACAACGTAG TCAACTTCAA 
GGAGCAATGC AGCAGTTTAA CCAGAGATTC ATTTATGGAA ATCAAGATTT GTTTGCTACA 
TCACAAAATA AAGAATTTGA TCCCCTTGGT CCTTTGCCAC CGGGATGGGA GAAGAGAACT 
GATAGCAATG GCAGAGTATA TTTTGTCAAC CACAACACTC GAATTACACA ATGGGAAGAC 
CCCAGAAATC AAGGTCAGTT AAATGAAAAG CCGTTACCTG AAGGCTGGGA AATGAGATTC 
ACAGTGGATG GAATTCCATA TTTTGTAGAC CACAATAAAA GAACAACTAC CTATATAGAT 
CCACGCACAG GAAAATCTGC CCTAGACAAT GGACCTCAGA TAGCCTATGT TCGGGACTTC 
AAGGCAAAGG TTCAGTATTT CCGGTTCTGG TGCCAGCAAT TGGCCATGCC ACAGCACATA 
AAGATTACAG TAACAAGAAA AACACTGTTT GAGGATTCTT TTCAACAGAT AATGAACTTC 
AGTCCCCAAG ATCTGCGAAG ACGATTGTGG GTGATTTTTC CAGGAGAAGA AGGTTTAGAT 
TATGGAGGTG TAGCAAGAGA GTGGTTCTTT CTTTTGTCAC ATGAAGTTTT GAACCCAATG 
TATTGCCTGT TTGAATATGC AGGGAAGGAT AACTATTGCC TGCAGATAAA CCCCGCTTCT 
TACATCAATC CAGATCACCT GAAATATTTT CGTTTTATTG GCAGGTTTAT TGCCATGGCT 
CTGTTCCATG GAAAATTCAT AGACACAGGT TTTTCTTTAC CATTCTATAA ATGTATCTTG 
AACAAACCAG TTGGACTTAA GGATTTAGAA TCTATTGATC CAGAATTTTA CAATTCTCTC 
ATCTGGGTTA AAGAAAACAA TATTGAAGAA TGTGGTTTGG AAATGTACTT CTCCGTTGAT 
AAAGAAATTC TAGGTGAAAT TAAAAGTCAT GACTTGAAAC CCAATGGTGG CAATATTCTT 
GTAACAGAAG AAAATAAAGA GGAATATATC AGAATGGTAG CTGAGTGGCG GTTATCTCGA 
GGTGTTGAAG AACAGACACA AGCTTTCTTT GAGGGTTTTA ATGAAATTCT TCCTCAGCAG 
TATTTGCAAT ACTTTGATGC AAAGGAATTA GAGGTCCTTT TATGTGGAAT GCAAGAGATT 
GATTTAAATG ACTGGCAAAG ACATGCAATC TACCGTCATT ACACTAGGAC AAGCAAACAA 
ATCATGTGGT TTTGGCAGTT TGTTAAGGAA ATTGATAATG AGAAGAGAAT GAGACTTCTG 
CAATTTGTCA CTGGAACTTG CCGATTACCA GTAGGAGGAT TTGCTGACCT CATGGGAAGC 
AATGGACCAC AGAAATTCTG CATTGAGAAA GTTGGGAAAG AAAATTGGTT ACCCAGAAGT 
CATACCTGTT TTAACCGCCT GGACCTTCCA CCCTACAAAA GCTACGAGCA ACTAAAGGAA 
AAGCTGTTGT TTGCCATTGA AGAAACAGAA GGATTTGGAC AAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itch ( XM_005329914.3 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005329971.1 Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OIc60905
Clone ID Related Accession (Same CDS sequence)	XM_005329914.2 , XM_005329914.3
Accession Version	XM_005329914.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936561.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_005329914.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGTAGCTT GATCATGAAG TCACAACTTC AGGTCACTGT CATTTCAGCA 
AAACTTAAAG AAAATAAAAA GAATTGGTTT GGACCAAGTC CTTATGTGGA AGTCACAGTG 
GATGGACAGT CAAAAAAGAC AGAAAAATGC AACAATACCA ACAGCCCCAA ATGGAAGCAA 
CCCCTTACAG TTATCGTTAC CCCTGTGAGT AAATTACATT TTCGAGTGTG GAGTCACCAG 
ACACTGAAGT CTGATGTTTT ATTGGGAACT GCTGCATTAG ATATTTATGA AACATTAAAG 
TCAAACAATA TGAAACTTGA AGAAGTGATT GTGACTTTGC AGCTTGCAGG TGACAAAGAG 
CCAACAGAGA CAATGGGAGA CTTGTCAATT TGTCTTGATG GGCTGCAGTT AGAAGCTGAA 
GTTGTTACTA ATGGTGAAAC TACATGTTCA GAAAGTACTT CACAGAATGA TGATGGCTCC 
AGATCCAAGG ATGAAACAAG AGTGAACACA AGTGGTTCAG ATGACCCTGA GGATGGAGGG 
TCTGGTGAAA ATAGAAGGGT CAATGGGAAC AATTCTCCAT CACTGTCAAA TGGTGGTTTT 
AAACCTTCTA GACCTCCAAG ACCTTCACGA CCACCTCCAC CAACCCCACG TAGACCAGCA 
TCTGTCAATG GTTCACCATC TACCACTTCT GAAAGTGATG GATCTAGTAC AGGTTCTCTG 
CCACCAACAA ATACAAATAC AAATACATCT GAAGGAGCAA CATCTGGATT AATAATTCCC 
CTTACTATAT CTGGAGGCTC AGGTCCTAGG CCATTGAATC CTGTAACTCA AGCTCCCCTA 
CCACCTGGTT GGGAGCAAAG AGTGAACCAG CATGGGCGAG TTTATTATGT AGATCATGTT 
GAGAAAAGAA CAACGTGGGA TAGACCTGAG CCTCTACCTC CTGGCTGGGA ACGTCGAGTT 
GACAATATGG GACGCATTTA TTATGTTGAC CATTTCACAA GAACAACAAC ATGGCAGAGG 
CCAACATTGG AATCTGTCCG GAACTACGAA CAGTGGCAGC TACAACGTAG TCAACTTCAA 
GGAGCAATGC AGCAGTTTAA CCAGAGATTC ATTTATGGAA ATCAAGATTT GTTTGCTACA 
TCACAAAATA AAGAATTTGA TCCCCTTGGT CCTTTGCCAC CGGGATGGGA GAAGAGAACT 
GATAGCAATG GCAGAGTATA TTTTGTCAAC CACAACACTC GAATTACACA ATGGGAAGAC 
CCCAGAAATC AAGGTCAGTT AAATGAAAAG CCGTTACCTG AAGGCTGGGA AATGAGATTC 
ACAGTGGATG GAATTCCATA TTTTGTAGAC CACAATAAAA GAACAACTAC CTATATAGAT 
CCACGCACAG GAAAATCTGC CCTAGACAAT GGACCTCAGA TAGCCTATGT TCGGGACTTC 
AAGGCAAAGG TTCAGTATTT CCGGTTCTGG TGCCAGCAAT TGGCCATGCC ACAGCACATA 
AAGATTACAG TAACAAGAAA AACACTGTTT GAGGATTCTT TTCAACAGAT AATGAACTTC 
AGTCCCCAAG ATCTGCGAAG ACGATTGTGG GTGATTTTTC CAGGAGAAGA AGGTTTAGAT 
TATGGAGGTG TAGCAAGAGA GTGGTTCTTT CTTTTGTCAC ATGAAGTTTT GAACCCAATG 
TATTGCCTGT TTGAATATGC AGGGAAGGAT AACTATTGCC TGCAGATAAA CCCCGCTTCT 
TACATCAATC CAGATCACCT GAAATATTTT CGTTTTATTG GCAGGTTTAT TGCCATGGCT 
CTGTTCCATG GAAAATTCAT AGACACAGGT TTTTCTTTAC CATTCTATAA ATGTATCTTG 
AACAAACCAG TTGGACTTAA GGATTTAGAA TCTATTGATC CAGAATTTTA CAATTCTCTC 
ATCTGGGTTA AAGAAAACAA TATTGAAGAA TGTGGTTTGG AAATGTACTT CTCCGTTGAT 
AAAGAAATTC TAGGTGAAAT TAAAAGTCAT GACTTGAAAC CCAATGGTGG CAATATTCTT 
GTAACAGAAG AAAATAAAGA GGAATATATC AGAATGGTAG CTGAGTGGCG GTTATCTCGA 
GGTGTTGAAG AACAGACACA AGCTTTCTTT GAGGGTTTTA ATGAAATTCT TCCTCAGCAG 
TATTTGCAAT ACTTTGATGC AAAGGAATTA GAGGTCCTTT TATGTGGAAT GCAAGAGATT 
GATTTAAATG ACTGGCAAAG ACATGCAATC TACCGTCATT ACACTAGGAC AAGCAAACAA 
ATCATGTGGT TTTGGCAGTT TGTTAAGGAA ATTGATAATG AGAAGAGAAT GAGACTTCTG 
CAATTTGTCA CTGGAACTTG CCGATTACCA GTAGGAGGAT TTGCTGACCT CATGGGAAGC 
AATGGACCAC AGAAATTCTG CATTGAGAAA GTTGGGAAAG AAAATTGGTT ACCCAGAAGT 
CATACCTGTT TTAACCGCCT GGACCTTCCA CCCTACAAAA GCTACGAGCA ACTAAAGGAA 
AAGCTGTTGT TTGCCATTGA AGAAACAGAA GGATTTGGAC AAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005329971.1
CDS	188..2755
Translation	MSDSGSLIMKSQLQVTVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVIVTLQLAGDKEPTETMGDLSICLDGLQLEAEVVTNGETTCSESTSQNDDGSRSKDETRVNTSGSDDPEDGGSGENRRVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTTSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVNQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRNQGQLNEKPLPEGWEMRFTVDGIPYFVDHNKRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMNFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKCILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_005329914.3
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005329914.3

ORF Insert Sequence:

ATGTCTGACA GTGGTAGCTT GATCATGAAG TCACAACTTC AGGTCACTGT CATTTCAGCA 
AAACTTAAAG AAAATAAAAA GAATTGGTTT GGACCAAGTC CTTATGTGGA AGTCACAGTG 
GATGGACAGT CAAAAAAGAC AGAAAAATGC AACAATACCA ACAGCCCCAA ATGGAAGCAA 
CCCCTTACAG TTATCGTTAC CCCTGTGAGT AAATTACATT TTCGAGTGTG GAGTCACCAG 
ACACTGAAGT CTGATGTTTT ATTGGGAACT GCTGCATTAG ATATTTATGA AACATTAAAG 
TCAAACAATA TGAAACTTGA AGAAGTGATT GTGACTTTGC AGCTTGCAGG TGACAAAGAG 
CCAACAGAGA CAATGGGAGA CTTGTCAATT TGTCTTGATG GGCTGCAGTT AGAAGCTGAA 
GTTGTTACTA ATGGTGAAAC TACATGTTCA GAAAGTACTT CACAGAATGA TGATGGCTCC 
AGATCCAAGG ATGAAACAAG AGTGAACACA AGTGGTTCAG ATGACCCTGA GGATGGAGGG 
TCTGGTGAAA ATAGAAGGGT CAATGGGAAC AATTCTCCAT CACTGTCAAA TGGTGGTTTT 
AAACCTTCTA GACCTCCAAG ACCTTCACGA CCACCTCCAC CAACCCCACG TAGACCAGCA 
TCTGTCAATG GTTCACCATC TACCACTTCT GAAAGTGATG GATCTAGTAC AGGTTCTCTG 
CCACCAACAA ATACAAATAC AAATACATCT GAAGGAGCAA CATCTGGATT AATAATTCCC 
CTTACTATAT CTGGAGGCTC AGGTCCTAGG CCATTGAATC CTGTAACTCA AGCTCCCCTA 
CCACCTGGTT GGGAGCAAAG AGTGAACCAG CATGGGCGAG TTTATTATGT AGATCATGTT 
GAGAAAAGAA CAACGTGGGA TAGACCTGAG CCTCTACCTC CTGGCTGGGA ACGTCGAGTT 
GACAATATGG GACGCATTTA TTATGTTGAC CATTTCACAA GAACAACAAC ATGGCAGAGG 
CCAACATTGG AATCTGTCCG GAACTACGAA CAGTGGCAGC TACAACGTAG TCAACTTCAA 
GGAGCAATGC AGCAGTTTAA CCAGAGATTC ATTTATGGAA ATCAAGATTT GTTTGCTACA 
TCACAAAATA AAGAATTTGA TCCCCTTGGT CCTTTGCCAC CGGGATGGGA GAAGAGAACT 
GATAGCAATG GCAGAGTATA TTTTGTCAAC CACAACACTC GAATTACACA ATGGGAAGAC 
CCCAGAAATC AAGGTCAGTT AAATGAAAAG CCGTTACCTG AAGGCTGGGA AATGAGATTC 
ACAGTGGATG GAATTCCATA TTTTGTAGAC CACAATAAAA GAACAACTAC CTATATAGAT 
CCACGCACAG GAAAATCTGC CCTAGACAAT GGACCTCAGA TAGCCTATGT TCGGGACTTC 
AAGGCAAAGG TTCAGTATTT CCGGTTCTGG TGCCAGCAAT TGGCCATGCC ACAGCACATA 
AAGATTACAG TAACAAGAAA AACACTGTTT GAGGATTCTT TTCAACAGAT AATGAACTTC 
AGTCCCCAAG ATCTGCGAAG ACGATTGTGG GTGATTTTTC CAGGAGAAGA AGGTTTAGAT 
TATGGAGGTG TAGCAAGAGA GTGGTTCTTT CTTTTGTCAC ATGAAGTTTT GAACCCAATG 
TATTGCCTGT TTGAATATGC AGGGAAGGAT AACTATTGCC TGCAGATAAA CCCCGCTTCT 
TACATCAATC CAGATCACCT GAAATATTTT CGTTTTATTG GCAGGTTTAT TGCCATGGCT 
CTGTTCCATG GAAAATTCAT AGACACAGGT TTTTCTTTAC CATTCTATAA ATGTATCTTG 
AACAAACCAG TTGGACTTAA GGATTTAGAA TCTATTGATC CAGAATTTTA CAATTCTCTC 
ATCTGGGTTA AAGAAAACAA TATTGAAGAA TGTGGTTTGG AAATGTACTT CTCCGTTGAT 
AAAGAAATTC TAGGTGAAAT TAAAAGTCAT GACTTGAAAC CCAATGGTGG CAATATTCTT 
GTAACAGAAG AAAATAAAGA GGAATATATC AGAATGGTAG CTGAGTGGCG GTTATCTCGA 
GGTGTTGAAG AACAGACACA AGCTTTCTTT GAGGGTTTTA ATGAAATTCT TCCTCAGCAG 
TATTTGCAAT ACTTTGATGC AAAGGAATTA GAGGTCCTTT TATGTGGAAT GCAAGAGATT 
GATTTAAATG ACTGGCAAAG ACATGCAATC TACCGTCATT ACACTAGGAC AAGCAAACAA 
ATCATGTGGT TTTGGCAGTT TGTTAAGGAA ATTGATAATG AGAAGAGAAT GAGACTTCTG 
CAATTTGTCA CTGGAACTTG CCGATTACCA GTAGGAGGAT TTGCTGACCT CATGGGAAGC 
AATGGACCAC AGAAATTCTG CATTGAGAAA GTTGGGAAAG AAAATTGGTT ACCCAGAAGT 
CATACCTGTT TTAACCGCCT GGACCTTCCA CCCTACAAAA GCTACGAGCA ACTAAAGGAA 
AAGCTGTTGT TTGCCATTGA AGAAACAGAA GGATTTGGAC AAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itch ( XM_021729578.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021585253.1 Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OIc80270
Clone ID Related Accession (Same CDS sequence)	XM_021729578.1 , XM_021729577.1 , XM_021729576.1
Accession Version	XM_021729578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGTAGCTT GATCATGAAG TCACAACTTC AGGTCACTGT CATTTCAGCA 
AAACTTAAAG AAAATAAAAA GAATTGGTTT GGACCAAGTC CTTATGTGGA AGTCACAGTG 
GATGGACAGT CAAAAAAGAC AGAAAAATGC AACAATACCA ACAGCCCCAA ATGGAAGCAA 
CCCCTTACAG TTATCGTTAC CCCTGTGAGT AAATTACATT TTCGAGTGTG GAGTCACCAG 
ACACTGAAGT CTGATGTTTT ATTGGGAACT GCTGCATTAG ATATTTATGA AACATTAAAG 
TCAAACAATA TGAAACTTGA AGAAGTGATT GTGACTTTGC AGCTTGCAGG TGACAAAGAG 
CCAACAGAGA CAATGGGAGA CTTGTCAATT TGTCTTGATG GGCTGCAGTT AGAAGCTGAA 
GTTGTTACTA ATGGTGAAAC TACATGTTCA GAAATTTCTG AGATTGCAGG TACTTCACAG 
AATGATGATG GCTCCAGATC CAAGGATGAA ACAAGAGTGA ACACAAGTGG TTCAGATGAC 
CCTGAGGATG GAGGGTCTGG TGAAAATAGA AGGGTCAATG GGAACAATTC TCCATCACTG 
TCAAATGGTG GTTTTAAACC TTCTAGACCT CCAAGACCTT CACGACCACC TCCACCAACC 
CCACGTAGAC CAGCATCTGT CAATGGTTCA CCATCTACCA CTTCTGAAAG TGATGGATCT 
AGTACAGGTT CTCTGCCACC AACAAATACA AATACAAATA CATCTGAAGG AGCAACATCT 
GGATTAATAA TTCCCCTTAC TATATCTGGA GGCTCAGGTC CTAGGCCATT GAATCCTGTA 
ACTCAAGCTC CCCTACCACC TGGTTGGGAG CAAAGAGTGA ACCAGCATGG GCGAGTTTAT 
TATGTAGATC ATGTTGAGAA AAGAACAACG TGGGATAGAC CTGAGCCTCT ACCTCCTGGC 
TGGGAACGTC GAGTTGACAA TATGGGACGC ATTTATTATG TTGACCATTT CACAAGAACA 
ACAACATGGC AGAGGCCAAC ATTGGAATCT GTCCGGAACT ACGAACAGTG GCAGCTACAA 
CGTAGTCAAC TTCAAGGAGC AATGCAGCAG TTTAACCAGA GATTCATTTA TGGAAATCAA 
GATTTGTTTG CTACATCACA AAATAAAGAA TTTGATCCCC TTGGTCCTTT GCCACCGGGA 
TGGGAGAAGA GAACTGATAG CAATGGCAGA GTATATTTTG TCAACCACAA CACTCGAATT 
ACACAATGGG AAGACCCCAG AAATCAAGGT CAGTTAAATG AAAAGCCGTT ACCTGAAGGC 
TGGGAAATGA GATTCACAGT GGATGGAATT CCATATTTTG TAGACCACAA TAAAAGAACA 
ACTACCTATA TAGATCCACG CACAGGAAAA TCTGCCCTAG ACAATGGACC TCAGATAGCC 
TATGTTCGGG ACTTCAAGGC AAAGGTTCAG TATTTCCGGT TCTGGTGCCA GCAATTGGCC 
ATGCCACAGC ACATAAAGAT TACAGTAACA AGAAAAACAC TGTTTGAGGA TTCTTTTCAA 
CAGATAATGA ACTTCAGTCC CCAAGATCTG CGAAGACGAT TGTGGGTGAT TTTTCCAGGA 
GAAGAAGGTT TAGATTATGG AGGTGTAGCA AGAGAGTGGT TCTTTCTTTT GTCACATGAA 
GTTTTGAACC CAATGTATTG CCTGTTTGAA TATGCAGGGA AGGATAACTA TTGCCTGCAG 
ATAAACCCCG CTTCTTACAT CAATCCAGAT CACCTGAAAT ATTTTCGTTT TATTGGCAGG 
TTTATTGCCA TGGCTCTGTT CCATGGAAAA TTCATAGACA CAGGTTTTTC TTTACCATTC 
TATAAATGTA TCTTGAACAA ACCAGTTGGA CTTAAGGATT TAGAATCTAT TGATCCAGAA 
TTTTACAATT CTCTCATCTG GGTTAAAGAA AACAATATTG AAGAATGTGG TTTGGAAATG 
TACTTCTCCG TTGATAAAGA AATTCTAGGT GAAATTAAAA GTCATGACTT GAAACCCAAT 
GGTGGCAATA TTCTTGTAAC AGAAGAAAAT AAAGAGGAAT ATATCAGAAT GGTAGCTGAG 
TGGCGGTTAT CTCGAGGTGT TGAAGAACAG ACACAAGCTT TCTTTGAGGG TTTTAATGAA 
ATTCTTCCTC AGCAGTATTT GCAATACTTT GATGCAAAGG AATTAGAGGT CCTTTTATGT 
GGAATGCAAG AGATTGATTT AAATGACTGG CAAAGACATG CAATCTACCG TCATTACACT 
AGGACAAGCA AACAAATCAT GTGGTTTTGG CAGTTTGTTA AGGAAATTGA TAATGAGAAG 
AGAATGAGAC TTCTGCAATT TGTCACTGGA ACTTGCCGAT TACCAGTAGG AGGATTTGCT 
GACCTCATGG GAAGCAATGG ACCACAGAAA TTCTGCATTG AGAAAGTTGG GAAAGAAAAT 
TGGTTACCCA GAAGTCATAC CTGTTTTAAC CGCCTGGACC TTCCACCCTA CAAAAGCTAC 
GAGCAACTAA AGGAAAAGCT GTTGTTTGCC ATTGAAGAAA CAGAAGGATT TGGACAAGAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021585253.1
CDS	51..2633
Translation	MSDSGSLIMKSQLQVTVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVIVTLQLAGDKEPTETMGDLSICLDGLQLEAEVVTNGETTCSEISEIAGTSQNDDGSRSKDETRVNTSGSDDPEDGGSGENRRVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTTSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVNQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRNQGQLNEKPLPEGWEMRFTVDGIPYFVDHNKRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMNFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKCILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_021729578.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021729578.1

ORF Insert Sequence:

ATGTCTGACA GTGGTAGCTT GATCATGAAG TCACAACTTC AGGTCACTGT CATTTCAGCA 
AAACTTAAAG AAAATAAAAA GAATTGGTTT GGACCAAGTC CTTATGTGGA AGTCACAGTG 
GATGGACAGT CAAAAAAGAC AGAAAAATGC AACAATACCA ACAGCCCCAA ATGGAAGCAA 
CCCCTTACAG TTATCGTTAC CCCTGTGAGT AAATTACATT TTCGAGTGTG GAGTCACCAG 
ACACTGAAGT CTGATGTTTT ATTGGGAACT GCTGCATTAG ATATTTATGA AACATTAAAG 
TCAAACAATA TGAAACTTGA AGAAGTGATT GTGACTTTGC AGCTTGCAGG TGACAAAGAG 
CCAACAGAGA CAATGGGAGA CTTGTCAATT TGTCTTGATG GGCTGCAGTT AGAAGCTGAA 
GTTGTTACTA ATGGTGAAAC TACATGTTCA GAAATTTCTG AGATTGCAGG TACTTCACAG 
AATGATGATG GCTCCAGATC CAAGGATGAA ACAAGAGTGA ACACAAGTGG TTCAGATGAC 
CCTGAGGATG GAGGGTCTGG TGAAAATAGA AGGGTCAATG GGAACAATTC TCCATCACTG 
TCAAATGGTG GTTTTAAACC TTCTAGACCT CCAAGACCTT CACGACCACC TCCACCAACC 
CCACGTAGAC CAGCATCTGT CAATGGTTCA CCATCTACCA CTTCTGAAAG TGATGGATCT 
AGTACAGGTT CTCTGCCACC AACAAATACA AATACAAATA CATCTGAAGG AGCAACATCT 
GGATTAATAA TTCCCCTTAC TATATCTGGA GGCTCAGGTC CTAGGCCATT GAATCCTGTA 
ACTCAAGCTC CCCTACCACC TGGTTGGGAG CAAAGAGTGA ACCAGCATGG GCGAGTTTAT 
TATGTAGATC ATGTTGAGAA AAGAACAACG TGGGATAGAC CTGAGCCTCT ACCTCCTGGC 
TGGGAACGTC GAGTTGACAA TATGGGACGC ATTTATTATG TTGACCATTT CACAAGAACA 
ACAACATGGC AGAGGCCAAC ATTGGAATCT GTCCGGAACT ACGAACAGTG GCAGCTACAA 
CGTAGTCAAC TTCAAGGAGC AATGCAGCAG TTTAACCAGA GATTCATTTA TGGAAATCAA 
GATTTGTTTG CTACATCACA AAATAAAGAA TTTGATCCCC TTGGTCCTTT GCCACCGGGA 
TGGGAGAAGA GAACTGATAG CAATGGCAGA GTATATTTTG TCAACCACAA CACTCGAATT 
ACACAATGGG AAGACCCCAG AAATCAAGGT CAGTTAAATG AAAAGCCGTT ACCTGAAGGC 
TGGGAAATGA GATTCACAGT GGATGGAATT CCATATTTTG TAGACCACAA TAAAAGAACA 
ACTACCTATA TAGATCCACG CACAGGAAAA TCTGCCCTAG ACAATGGACC TCAGATAGCC 
TATGTTCGGG ACTTCAAGGC AAAGGTTCAG TATTTCCGGT TCTGGTGCCA GCAATTGGCC 
ATGCCACAGC ACATAAAGAT TACAGTAACA AGAAAAACAC TGTTTGAGGA TTCTTTTCAA 
CAGATAATGA ACTTCAGTCC CCAAGATCTG CGAAGACGAT TGTGGGTGAT TTTTCCAGGA 
GAAGAAGGTT TAGATTATGG AGGTGTAGCA AGAGAGTGGT TCTTTCTTTT GTCACATGAA 
GTTTTGAACC CAATGTATTG CCTGTTTGAA TATGCAGGGA AGGATAACTA TTGCCTGCAG 
ATAAACCCCG CTTCTTACAT CAATCCAGAT CACCTGAAAT ATTTTCGTTT TATTGGCAGG 
TTTATTGCCA TGGCTCTGTT CCATGGAAAA TTCATAGACA CAGGTTTTTC TTTACCATTC 
TATAAATGTA TCTTGAACAA ACCAGTTGGA CTTAAGGATT TAGAATCTAT TGATCCAGAA 
TTTTACAATT CTCTCATCTG GGTTAAAGAA AACAATATTG AAGAATGTGG TTTGGAAATG 
TACTTCTCCG TTGATAAAGA AATTCTAGGT GAAATTAAAA GTCATGACTT GAAACCCAAT 
GGTGGCAATA TTCTTGTAAC AGAAGAAAAT AAAGAGGAAT ATATCAGAAT GGTAGCTGAG 
TGGCGGTTAT CTCGAGGTGT TGAAGAACAG ACACAAGCTT TCTTTGAGGG TTTTAATGAA 
ATTCTTCCTC AGCAGTATTT GCAATACTTT GATGCAAAGG AATTAGAGGT CCTTTTATGT 
GGAATGCAAG AGATTGATTT AAATGACTGG CAAAGACATG CAATCTACCG TCATTACACT 
AGGACAAGCA AACAAATCAT GTGGTTTTGG CAGTTTGTTA AGGAAATTGA TAATGAGAAG 
AGAATGAGAC TTCTGCAATT TGTCACTGGA ACTTGCCGAT TACCAGTAGG AGGATTTGCT 
GACCTCATGG GAAGCAATGG ACCACAGAAA TTCTGCATTG AGAAAGTTGG GAAAGAAAAT 
TGGTTACCCA GAAGTCATAC CTGTTTTAAC CGCCTGGACC TTCCACCCTA CAAAAGCTAC 
GAGCAACTAA AGGAAAAGCT GTTGTTTGCC ATTGAAGAAA CAGAAGGATT TGGACAAGAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itch ( XM_021729577.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021585252.1 Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OIc80270
Clone ID Related Accession (Same CDS sequence)	XM_021729578.1 , XM_021729577.1 , XM_021729576.1
Accession Version	XM_021729577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGTAGCTT GATCATGAAG TCACAACTTC AGGTCACTGT CATTTCAGCA 
AAACTTAAAG AAAATAAAAA GAATTGGTTT GGACCAAGTC CTTATGTGGA AGTCACAGTG 
GATGGACAGT CAAAAAAGAC AGAAAAATGC AACAATACCA ACAGCCCCAA ATGGAAGCAA 
CCCCTTACAG TTATCGTTAC CCCTGTGAGT AAATTACATT TTCGAGTGTG GAGTCACCAG 
ACACTGAAGT CTGATGTTTT ATTGGGAACT GCTGCATTAG ATATTTATGA AACATTAAAG 
TCAAACAATA TGAAACTTGA AGAAGTGATT GTGACTTTGC AGCTTGCAGG TGACAAAGAG 
CCAACAGAGA CAATGGGAGA CTTGTCAATT TGTCTTGATG GGCTGCAGTT AGAAGCTGAA 
GTTGTTACTA ATGGTGAAAC TACATGTTCA GAAATTTCTG AGATTGCAGG TACTTCACAG 
AATGATGATG GCTCCAGATC CAAGGATGAA ACAAGAGTGA ACACAAGTGG TTCAGATGAC 
CCTGAGGATG GAGGGTCTGG TGAAAATAGA AGGGTCAATG GGAACAATTC TCCATCACTG 
TCAAATGGTG GTTTTAAACC TTCTAGACCT CCAAGACCTT CACGACCACC TCCACCAACC 
CCACGTAGAC CAGCATCTGT CAATGGTTCA CCATCTACCA CTTCTGAAAG TGATGGATCT 
AGTACAGGTT CTCTGCCACC AACAAATACA AATACAAATA CATCTGAAGG AGCAACATCT 
GGATTAATAA TTCCCCTTAC TATATCTGGA GGCTCAGGTC CTAGGCCATT GAATCCTGTA 
ACTCAAGCTC CCCTACCACC TGGTTGGGAG CAAAGAGTGA ACCAGCATGG GCGAGTTTAT 
TATGTAGATC ATGTTGAGAA AAGAACAACG TGGGATAGAC CTGAGCCTCT ACCTCCTGGC 
TGGGAACGTC GAGTTGACAA TATGGGACGC ATTTATTATG TTGACCATTT CACAAGAACA 
ACAACATGGC AGAGGCCAAC ATTGGAATCT GTCCGGAACT ACGAACAGTG GCAGCTACAA 
CGTAGTCAAC TTCAAGGAGC AATGCAGCAG TTTAACCAGA GATTCATTTA TGGAAATCAA 
GATTTGTTTG CTACATCACA AAATAAAGAA TTTGATCCCC TTGGTCCTTT GCCACCGGGA 
TGGGAGAAGA GAACTGATAG CAATGGCAGA GTATATTTTG TCAACCACAA CACTCGAATT 
ACACAATGGG AAGACCCCAG AAATCAAGGT CAGTTAAATG AAAAGCCGTT ACCTGAAGGC 
TGGGAAATGA GATTCACAGT GGATGGAATT CCATATTTTG TAGACCACAA TAAAAGAACA 
ACTACCTATA TAGATCCACG CACAGGAAAA TCTGCCCTAG ACAATGGACC TCAGATAGCC 
TATGTTCGGG ACTTCAAGGC AAAGGTTCAG TATTTCCGGT TCTGGTGCCA GCAATTGGCC 
ATGCCACAGC ACATAAAGAT TACAGTAACA AGAAAAACAC TGTTTGAGGA TTCTTTTCAA 
CAGATAATGA ACTTCAGTCC CCAAGATCTG CGAAGACGAT TGTGGGTGAT TTTTCCAGGA 
GAAGAAGGTT TAGATTATGG AGGTGTAGCA AGAGAGTGGT TCTTTCTTTT GTCACATGAA 
GTTTTGAACC CAATGTATTG CCTGTTTGAA TATGCAGGGA AGGATAACTA TTGCCTGCAG 
ATAAACCCCG CTTCTTACAT CAATCCAGAT CACCTGAAAT ATTTTCGTTT TATTGGCAGG 
TTTATTGCCA TGGCTCTGTT CCATGGAAAA TTCATAGACA CAGGTTTTTC TTTACCATTC 
TATAAATGTA TCTTGAACAA ACCAGTTGGA CTTAAGGATT TAGAATCTAT TGATCCAGAA 
TTTTACAATT CTCTCATCTG GGTTAAAGAA AACAATATTG AAGAATGTGG TTTGGAAATG 
TACTTCTCCG TTGATAAAGA AATTCTAGGT GAAATTAAAA GTCATGACTT GAAACCCAAT 
GGTGGCAATA TTCTTGTAAC AGAAGAAAAT AAAGAGGAAT ATATCAGAAT GGTAGCTGAG 
TGGCGGTTAT CTCGAGGTGT TGAAGAACAG ACACAAGCTT TCTTTGAGGG TTTTAATGAA 
ATTCTTCCTC AGCAGTATTT GCAATACTTT GATGCAAAGG AATTAGAGGT CCTTTTATGT 
GGAATGCAAG AGATTGATTT AAATGACTGG CAAAGACATG CAATCTACCG TCATTACACT 
AGGACAAGCA AACAAATCAT GTGGTTTTGG CAGTTTGTTA AGGAAATTGA TAATGAGAAG 
AGAATGAGAC TTCTGCAATT TGTCACTGGA ACTTGCCGAT TACCAGTAGG AGGATTTGCT 
GACCTCATGG GAAGCAATGG ACCACAGAAA TTCTGCATTG AGAAAGTTGG GAAAGAAAAT 
TGGTTACCCA GAAGTCATAC CTGTTTTAAC CGCCTGGACC TTCCACCCTA CAAAAGCTAC 
GAGCAACTAA AGGAAAAGCT GTTGTTTGCC ATTGAAGAAA CAGAAGGATT TGGACAAGAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021585252.1
CDS	125..2707
Translation	MSDSGSLIMKSQLQVTVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVIVTLQLAGDKEPTETMGDLSICLDGLQLEAEVVTNGETTCSEISEIAGTSQNDDGSRSKDETRVNTSGSDDPEDGGSGENRRVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTTSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVNQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRNQGQLNEKPLPEGWEMRFTVDGIPYFVDHNKRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMNFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKCILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_021729577.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021729577.1

ORF Insert Sequence:

ATGTCTGACA GTGGTAGCTT GATCATGAAG TCACAACTTC AGGTCACTGT CATTTCAGCA 
AAACTTAAAG AAAATAAAAA GAATTGGTTT GGACCAAGTC CTTATGTGGA AGTCACAGTG 
GATGGACAGT CAAAAAAGAC AGAAAAATGC AACAATACCA ACAGCCCCAA ATGGAAGCAA 
CCCCTTACAG TTATCGTTAC CCCTGTGAGT AAATTACATT TTCGAGTGTG GAGTCACCAG 
ACACTGAAGT CTGATGTTTT ATTGGGAACT GCTGCATTAG ATATTTATGA AACATTAAAG 
TCAAACAATA TGAAACTTGA AGAAGTGATT GTGACTTTGC AGCTTGCAGG TGACAAAGAG 
CCAACAGAGA CAATGGGAGA CTTGTCAATT TGTCTTGATG GGCTGCAGTT AGAAGCTGAA 
GTTGTTACTA ATGGTGAAAC TACATGTTCA GAAATTTCTG AGATTGCAGG TACTTCACAG 
AATGATGATG GCTCCAGATC CAAGGATGAA ACAAGAGTGA ACACAAGTGG TTCAGATGAC 
CCTGAGGATG GAGGGTCTGG TGAAAATAGA AGGGTCAATG GGAACAATTC TCCATCACTG 
TCAAATGGTG GTTTTAAACC TTCTAGACCT CCAAGACCTT CACGACCACC TCCACCAACC 
CCACGTAGAC CAGCATCTGT CAATGGTTCA CCATCTACCA CTTCTGAAAG TGATGGATCT 
AGTACAGGTT CTCTGCCACC AACAAATACA AATACAAATA CATCTGAAGG AGCAACATCT 
GGATTAATAA TTCCCCTTAC TATATCTGGA GGCTCAGGTC CTAGGCCATT GAATCCTGTA 
ACTCAAGCTC CCCTACCACC TGGTTGGGAG CAAAGAGTGA ACCAGCATGG GCGAGTTTAT 
TATGTAGATC ATGTTGAGAA AAGAACAACG TGGGATAGAC CTGAGCCTCT ACCTCCTGGC 
TGGGAACGTC GAGTTGACAA TATGGGACGC ATTTATTATG TTGACCATTT CACAAGAACA 
ACAACATGGC AGAGGCCAAC ATTGGAATCT GTCCGGAACT ACGAACAGTG GCAGCTACAA 
CGTAGTCAAC TTCAAGGAGC AATGCAGCAG TTTAACCAGA GATTCATTTA TGGAAATCAA 
GATTTGTTTG CTACATCACA AAATAAAGAA TTTGATCCCC TTGGTCCTTT GCCACCGGGA 
TGGGAGAAGA GAACTGATAG CAATGGCAGA GTATATTTTG TCAACCACAA CACTCGAATT 
ACACAATGGG AAGACCCCAG AAATCAAGGT CAGTTAAATG AAAAGCCGTT ACCTGAAGGC 
TGGGAAATGA GATTCACAGT GGATGGAATT CCATATTTTG TAGACCACAA TAAAAGAACA 
ACTACCTATA TAGATCCACG CACAGGAAAA TCTGCCCTAG ACAATGGACC TCAGATAGCC 
TATGTTCGGG ACTTCAAGGC AAAGGTTCAG TATTTCCGGT TCTGGTGCCA GCAATTGGCC 
ATGCCACAGC ACATAAAGAT TACAGTAACA AGAAAAACAC TGTTTGAGGA TTCTTTTCAA 
CAGATAATGA ACTTCAGTCC CCAAGATCTG CGAAGACGAT TGTGGGTGAT TTTTCCAGGA 
GAAGAAGGTT TAGATTATGG AGGTGTAGCA AGAGAGTGGT TCTTTCTTTT GTCACATGAA 
GTTTTGAACC CAATGTATTG CCTGTTTGAA TATGCAGGGA AGGATAACTA TTGCCTGCAG 
ATAAACCCCG CTTCTTACAT CAATCCAGAT CACCTGAAAT ATTTTCGTTT TATTGGCAGG 
TTTATTGCCA TGGCTCTGTT CCATGGAAAA TTCATAGACA CAGGTTTTTC TTTACCATTC 
TATAAATGTA TCTTGAACAA ACCAGTTGGA CTTAAGGATT TAGAATCTAT TGATCCAGAA 
TTTTACAATT CTCTCATCTG GGTTAAAGAA AACAATATTG AAGAATGTGG TTTGGAAATG 
TACTTCTCCG TTGATAAAGA AATTCTAGGT GAAATTAAAA GTCATGACTT GAAACCCAAT 
GGTGGCAATA TTCTTGTAAC AGAAGAAAAT AAAGAGGAAT ATATCAGAAT GGTAGCTGAG 
TGGCGGTTAT CTCGAGGTGT TGAAGAACAG ACACAAGCTT TCTTTGAGGG TTTTAATGAA 
ATTCTTCCTC AGCAGTATTT GCAATACTTT GATGCAAAGG AATTAGAGGT CCTTTTATGT 
GGAATGCAAG AGATTGATTT AAATGACTGG CAAAGACATG CAATCTACCG TCATTACACT 
AGGACAAGCA AACAAATCAT GTGGTTTTGG CAGTTTGTTA AGGAAATTGA TAATGAGAAG 
AGAATGAGAC TTCTGCAATT TGTCACTGGA ACTTGCCGAT TACCAGTAGG AGGATTTGCT 
GACCTCATGG GAAGCAATGG ACCACAGAAA TTCTGCATTG AGAAAGTTGG GAAAGAAAAT 
TGGTTACCCA GAAGTCATAC CTGTTTTAAC CGCCTGGACC TTCCACCCTA CAAAAGCTAC 
GAGCAACTAA AGGAAAAGCT GTTGTTTGCC ATTGAAGAAA CAGAAGGATT TGGACAAGAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itch ( XM_021729576.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021585251.1 Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), transcript variant X1, mRNA.

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CloneID	OIc80270
Clone ID Related Accession (Same CDS sequence)	XM_021729578.1 , XM_021729577.1 , XM_021729576.1
Accession Version	XM_021729576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGGTAGCTT GATCATGAAG TCACAACTTC AGGTCACTGT CATTTCAGCA 
AAACTTAAAG AAAATAAAAA GAATTGGTTT GGACCAAGTC CTTATGTGGA AGTCACAGTG 
GATGGACAGT CAAAAAAGAC AGAAAAATGC AACAATACCA ACAGCCCCAA ATGGAAGCAA 
CCCCTTACAG TTATCGTTAC CCCTGTGAGT AAATTACATT TTCGAGTGTG GAGTCACCAG 
ACACTGAAGT CTGATGTTTT ATTGGGAACT GCTGCATTAG ATATTTATGA AACATTAAAG 
TCAAACAATA TGAAACTTGA AGAAGTGATT GTGACTTTGC AGCTTGCAGG TGACAAAGAG 
CCAACAGAGA CAATGGGAGA CTTGTCAATT TGTCTTGATG GGCTGCAGTT AGAAGCTGAA 
GTTGTTACTA ATGGTGAAAC TACATGTTCA GAAATTTCTG AGATTGCAGG TACTTCACAG 
AATGATGATG GCTCCAGATC CAAGGATGAA ACAAGAGTGA ACACAAGTGG TTCAGATGAC 
CCTGAGGATG GAGGGTCTGG TGAAAATAGA AGGGTCAATG GGAACAATTC TCCATCACTG 
TCAAATGGTG GTTTTAAACC TTCTAGACCT CCAAGACCTT CACGACCACC TCCACCAACC 
CCACGTAGAC CAGCATCTGT CAATGGTTCA CCATCTACCA CTTCTGAAAG TGATGGATCT 
AGTACAGGTT CTCTGCCACC AACAAATACA AATACAAATA CATCTGAAGG AGCAACATCT 
GGATTAATAA TTCCCCTTAC TATATCTGGA GGCTCAGGTC CTAGGCCATT GAATCCTGTA 
ACTCAAGCTC CCCTACCACC TGGTTGGGAG CAAAGAGTGA ACCAGCATGG GCGAGTTTAT 
TATGTAGATC ATGTTGAGAA AAGAACAACG TGGGATAGAC CTGAGCCTCT ACCTCCTGGC 
TGGGAACGTC GAGTTGACAA TATGGGACGC ATTTATTATG TTGACCATTT CACAAGAACA 
ACAACATGGC AGAGGCCAAC ATTGGAATCT GTCCGGAACT ACGAACAGTG GCAGCTACAA 
CGTAGTCAAC TTCAAGGAGC AATGCAGCAG TTTAACCAGA GATTCATTTA TGGAAATCAA 
GATTTGTTTG CTACATCACA AAATAAAGAA TTTGATCCCC TTGGTCCTTT GCCACCGGGA 
TGGGAGAAGA GAACTGATAG CAATGGCAGA GTATATTTTG TCAACCACAA CACTCGAATT 
ACACAATGGG AAGACCCCAG AAATCAAGGT CAGTTAAATG AAAAGCCGTT ACCTGAAGGC 
TGGGAAATGA GATTCACAGT GGATGGAATT CCATATTTTG TAGACCACAA TAAAAGAACA 
ACTACCTATA TAGATCCACG CACAGGAAAA TCTGCCCTAG ACAATGGACC TCAGATAGCC 
TATGTTCGGG ACTTCAAGGC AAAGGTTCAG TATTTCCGGT TCTGGTGCCA GCAATTGGCC 
ATGCCACAGC ACATAAAGAT TACAGTAACA AGAAAAACAC TGTTTGAGGA TTCTTTTCAA 
CAGATAATGA ACTTCAGTCC CCAAGATCTG CGAAGACGAT TGTGGGTGAT TTTTCCAGGA 
GAAGAAGGTT TAGATTATGG AGGTGTAGCA AGAGAGTGGT TCTTTCTTTT GTCACATGAA 
GTTTTGAACC CAATGTATTG CCTGTTTGAA TATGCAGGGA AGGATAACTA TTGCCTGCAG 
ATAAACCCCG CTTCTTACAT CAATCCAGAT CACCTGAAAT ATTTTCGTTT TATTGGCAGG 
TTTATTGCCA TGGCTCTGTT CCATGGAAAA TTCATAGACA CAGGTTTTTC TTTACCATTC 
TATAAATGTA TCTTGAACAA ACCAGTTGGA CTTAAGGATT TAGAATCTAT TGATCCAGAA 
TTTTACAATT CTCTCATCTG GGTTAAAGAA AACAATATTG AAGAATGTGG TTTGGAAATG 
TACTTCTCCG TTGATAAAGA AATTCTAGGT GAAATTAAAA GTCATGACTT GAAACCCAAT 
GGTGGCAATA TTCTTGTAAC AGAAGAAAAT AAAGAGGAAT ATATCAGAAT GGTAGCTGAG 
TGGCGGTTAT CTCGAGGTGT TGAAGAACAG ACACAAGCTT TCTTTGAGGG TTTTAATGAA 
ATTCTTCCTC AGCAGTATTT GCAATACTTT GATGCAAAGG AATTAGAGGT CCTTTTATGT 
GGAATGCAAG AGATTGATTT AAATGACTGG CAAAGACATG CAATCTACCG TCATTACACT 
AGGACAAGCA AACAAATCAT GTGGTTTTGG CAGTTTGTTA AGGAAATTGA TAATGAGAAG 
AGAATGAGAC TTCTGCAATT TGTCACTGGA ACTTGCCGAT TACCAGTAGG AGGATTTGCT 
GACCTCATGG GAAGCAATGG ACCACAGAAA TTCTGCATTG AGAAAGTTGG GAAAGAAAAT 
TGGTTACCCA GAAGTCATAC CTGTTTTAAC CGCCTGGACC TTCCACCCTA CAAAAGCTAC 
GAGCAACTAA AGGAAAAGCT GTTGTTTGCC ATTGAAGAAA CAGAAGGATT TGGACAAGAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021585251.1
CDS	188..2770
Translation	MSDSGSLIMKSQLQVTVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVIVTLQLAGDKEPTETMGDLSICLDGLQLEAEVVTNGETTCSEISEIAGTSQNDDGSRSKDETRVNTSGSDDPEDGGSGENRRVNGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTTSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVNQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRNQGQLNEKPLPEGWEMRFTVDGIPYFVDHNKRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMNFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKCILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_021729576.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus itchy E3 ubiquitin protein ligase (Itch), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021729576.1

ORF Insert Sequence:

ATGTCTGACA GTGGTAGCTT GATCATGAAG TCACAACTTC AGGTCACTGT CATTTCAGCA 
AAACTTAAAG AAAATAAAAA GAATTGGTTT GGACCAAGTC CTTATGTGGA AGTCACAGTG 
GATGGACAGT CAAAAAAGAC AGAAAAATGC AACAATACCA ACAGCCCCAA ATGGAAGCAA 
CCCCTTACAG TTATCGTTAC CCCTGTGAGT AAATTACATT TTCGAGTGTG GAGTCACCAG 
ACACTGAAGT CTGATGTTTT ATTGGGAACT GCTGCATTAG ATATTTATGA AACATTAAAG 
TCAAACAATA TGAAACTTGA AGAAGTGATT GTGACTTTGC AGCTTGCAGG TGACAAAGAG 
CCAACAGAGA CAATGGGAGA CTTGTCAATT TGTCTTGATG GGCTGCAGTT AGAAGCTGAA 
GTTGTTACTA ATGGTGAAAC TACATGTTCA GAAATTTCTG AGATTGCAGG TACTTCACAG 
AATGATGATG GCTCCAGATC CAAGGATGAA ACAAGAGTGA ACACAAGTGG TTCAGATGAC 
CCTGAGGATG GAGGGTCTGG TGAAAATAGA AGGGTCAATG GGAACAATTC TCCATCACTG 
TCAAATGGTG GTTTTAAACC TTCTAGACCT CCAAGACCTT CACGACCACC TCCACCAACC 
CCACGTAGAC CAGCATCTGT CAATGGTTCA CCATCTACCA CTTCTGAAAG TGATGGATCT 
AGTACAGGTT CTCTGCCACC AACAAATACA AATACAAATA CATCTGAAGG AGCAACATCT 
GGATTAATAA TTCCCCTTAC TATATCTGGA GGCTCAGGTC CTAGGCCATT GAATCCTGTA 
ACTCAAGCTC CCCTACCACC TGGTTGGGAG CAAAGAGTGA ACCAGCATGG GCGAGTTTAT 
TATGTAGATC ATGTTGAGAA AAGAACAACG TGGGATAGAC CTGAGCCTCT ACCTCCTGGC 
TGGGAACGTC GAGTTGACAA TATGGGACGC ATTTATTATG TTGACCATTT CACAAGAACA 
ACAACATGGC AGAGGCCAAC ATTGGAATCT GTCCGGAACT ACGAACAGTG GCAGCTACAA 
CGTAGTCAAC TTCAAGGAGC AATGCAGCAG TTTAACCAGA GATTCATTTA TGGAAATCAA 
GATTTGTTTG CTACATCACA AAATAAAGAA TTTGATCCCC TTGGTCCTTT GCCACCGGGA 
TGGGAGAAGA GAACTGATAG CAATGGCAGA GTATATTTTG TCAACCACAA CACTCGAATT 
ACACAATGGG AAGACCCCAG AAATCAAGGT CAGTTAAATG AAAAGCCGTT ACCTGAAGGC 
TGGGAAATGA GATTCACAGT GGATGGAATT CCATATTTTG TAGACCACAA TAAAAGAACA 
ACTACCTATA TAGATCCACG CACAGGAAAA TCTGCCCTAG ACAATGGACC TCAGATAGCC 
TATGTTCGGG ACTTCAAGGC AAAGGTTCAG TATTTCCGGT TCTGGTGCCA GCAATTGGCC 
ATGCCACAGC ACATAAAGAT TACAGTAACA AGAAAAACAC TGTTTGAGGA TTCTTTTCAA 
CAGATAATGA ACTTCAGTCC CCAAGATCTG CGAAGACGAT TGTGGGTGAT TTTTCCAGGA 
GAAGAAGGTT TAGATTATGG AGGTGTAGCA AGAGAGTGGT TCTTTCTTTT GTCACATGAA 
GTTTTGAACC CAATGTATTG CCTGTTTGAA TATGCAGGGA AGGATAACTA TTGCCTGCAG 
ATAAACCCCG CTTCTTACAT CAATCCAGAT CACCTGAAAT ATTTTCGTTT TATTGGCAGG 
TTTATTGCCA TGGCTCTGTT CCATGGAAAA TTCATAGACA CAGGTTTTTC TTTACCATTC 
TATAAATGTA TCTTGAACAA ACCAGTTGGA CTTAAGGATT TAGAATCTAT TGATCCAGAA 
TTTTACAATT CTCTCATCTG GGTTAAAGAA AACAATATTG AAGAATGTGG TTTGGAAATG 
TACTTCTCCG TTGATAAAGA AATTCTAGGT GAAATTAAAA GTCATGACTT GAAACCCAAT 
GGTGGCAATA TTCTTGTAAC AGAAGAAAAT AAAGAGGAAT ATATCAGAAT GGTAGCTGAG 
TGGCGGTTAT CTCGAGGTGT TGAAGAACAG ACACAAGCTT TCTTTGAGGG TTTTAATGAA 
ATTCTTCCTC AGCAGTATTT GCAATACTTT GATGCAAAGG AATTAGAGGT CCTTTTATGT 
GGAATGCAAG AGATTGATTT AAATGACTGG CAAAGACATG CAATCTACCG TCATTACACT 
AGGACAAGCA AACAAATCAT GTGGTTTTGG CAGTTTGTTA AGGAAATTGA TAATGAGAAG 
AGAATGAGAC TTCTGCAATT TGTCACTGGA ACTTGCCGAT TACCAGTAGG AGGATTTGCT 
GACCTCATGG GAAGCAATGG ACCACAGAAA TTCTGCATTG AGAAAGTTGG GAAAGAAAAT 
TGGTTACCCA GAAGTCATAC CTGTTTTAAC CGCCTGGACC TTCCACCCTA CAAAAGCTAC 
GAGCAACTAA AGGAAAAGCT GTTGTTTGCC ATTGAAGAAA CAGAAGGATT TGGACAAGAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Itch cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム