Unc5b cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene
Clones
gRNAs

Summary

Gene Symbol	Unc5b
Entrez Gene ID	101977649
Full Name	unc-5 netrin receptor B
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005325939.2	XP_005325996.1	netrin receptor UNC5B
XM_005325939.3	XP_005325996.1	netrin receptor UNC5B

Pathways from BioSystems
Gene Ontology

The following Unc5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Unc5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIc57109	XM_005325939.3 Latest version!	Ictidomys tridecemlineatus unc-5 netrin receptor B (Unc5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
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OIc57109	XM_005325939.2	Ictidomys tridecemlineatus unc-5 netrin receptor B (Unc5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Unc5b ( XM_005325939.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005325996.1 Ictidomys tridecemlineatus unc-5 netrin receptor B (Unc5b), mRNA.

Price & Availability↑

CloneID	OIc57109
Clone ID Related Accession (Same CDS sequence)	XM_005325939.2 , XM_005325939.3
Accession Version	XM_005325939.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-11
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	netrin receptor UNC5B
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936521.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Aug 12, 2015 this sequence version replaced XM_005325939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCC GCAGCGGAAC GCCGGGCGCT TTGCTGCTGG CGCTGCTACT CTGCTGGGAC 
CCGAGGCTGA GCCAAGCAGG CCCTGACCCC GGCAGCCAGG TGCTGCCCGA CTCCTTCCCG 
TCGGCGCCGG CCGAGCCGCT GCCCCACTTC CTGCAGGAGC CGCAGGACGC CTACCTGGTG 
AAGAGCAAGC CCGTGGAGCT GCGCTGCCGC GCCCGCCCCG CCACGCAGGT CTACTTCAAG 
TGCAACGGCG AGTGGGTCGG CCAGAAGGAC CACGCCACGC AGGAGGGGCT GGACGAGGCC 
ACCGGCCTGC GGGTGCGCGA GGTGCAGATC GAGGTGTCGC GGCAGCAGGT GGAGGAGCTC 
TTTGGGCTGG AGGATTACTG GTGCCAGTGT GTGGCCTGGA GCTCCGCGGG CACCACCAAG 
AGTCGCCGGG CCTACGTGCG CATCGCCTAC CTGCGCAAGA ACTTCGACCA GGAGCCTCTG 
GGCAAGGAAG TGCCCCTGGA CCACGAGGTT CTCCTGCAGT GCCGCCCCCC AGAGGGGGTG 
CCTGTGGCCG AGGTGGAATG GCTCAGGAAT GAGGATGTCA TCGACCCCAC CCAGGACACC 
AACTTCCTGA TCACCATCGA CCACAACCTC ATCATCCGCC AGGCCCGCCT GTCGGACACG 
GCCAACTACA CCTGCGTGGC CAAGAACATC GTGGCCAAGC GCCGGAGCAC CACGGCCACC 
GTCATCGTCT ACGTGAATGG CGGCTGGTCC AGCTGGGCCG AGTGGTCGCC CTGCTCCAAC 
CGCTGTGGCC GAGGCTGGCA GAAGCGCACC CGGACCTGCA CCAACCCCGC CCCGCTCAAC 
GGAGGTGCCT TCTGCGAGGG CCAGGCCTTC CAGAAAACCG CCTGCACCAC CGTGTGCCCA 
GTGGACGGGG CCTGGACAGA GTGGAGCAAG TGGTCGGCCT GCAGCACCGA GTGTGCCCAC 
TGGCGCAGCC GCGAGTGCAT GGCGCCCCCG CCCCAGAACG GAGGCCGCGA CTGCAGCGGG 
ACCCTGCTGG ACTCCAAGAA CTGCACAGAC GGGCTGTGCA TGCAGAATAA GAGGACTCTA 
AGCGACCCCA AAAGCCACCT CCTGGAGGCC TCGGGAGATG TGGCCCTGTA CGCGGGCCTC 
GTGGTGGCCA TCTTGGTGGT GGTGGCGGTC CTCATGGCCG TGGGCGCCGT GGTGTACCGT 
CGCAACTGTC GCGACTTCGA CACGGACATC ACGGACTCGT CCGCTGCCCT CACTGGGGGC 
TTTCATCCAG TCAACTTCAA GACAGCAAGG CCCAGCAACC CTCAGCTCCT GCACCCCTCG 
GTGCCCCCCG ACCTGACAGC CAGCGCCGGC GCCTACCGCG GGCCTGTGTA TGCCCTGCAG 
GATGCGGCCG ACAAGATCCC CATGACCAAC TCGCCCCTGC TCGACCCCCT GCCCAGCCTC 
AAGATCAAGG TCTACAGCTC TAGCACCACT AGCTCCGGGC CCGGCCGGCC AGACGGGGCC 
GACCTGCTGG GGGTCACCTA CCCGCCGGGC ACCTACCCTG GCGACTTCTC CCGGGACGCA 
CACTTCCTGC ACCTGCACAG CGCCAGCCTG GGCTCCCAGC AGCCCCTGGG CCTGCCCCGG 
GACCCGGGCA GCAGCGTCAG CGGCACCTTC GGTTGCCTGG GCGGGAGGCT CAGCATCCCA 
GGCACAGGGG TCAGCCTGCT GGTGCCCAAT GGAGCCATTC CCCAGGGCAA GTTCTACGAG 
ATGTACCTGC TTATCAACAA GGCGGAAAGC ACCCTCCCGC TGTCAGAAGG GACCCAGACA 
GTACTGAGCC CCGCGGTGAC CTGCGGGCCA ACGGGCCTCC TGCTGTGCCG CCCGGTCATC 
CTCACGGTGC CCCACTGTGC TGAGGTCACT GCTGGCGACT GGATCTTCCA GCTCAAGACC 
CAGGCCCACC AGGGCCACTG GGAGGAGGTG GTGACCCTGG ACGAGGAGAC CCTGAACACC 
CCCTGCTACT GCCAGCTGGA GGCCAGGTCC TGCCACATCC TGCTGGACCA GCTGGGCACC 
TACGCCTTCA CGGGCGAGTC CTACTCCCGC GCGGCCTGCA AGCGGCTGCA GCTGGCCGTC 
TTCGCCCCGG CCCTGTGCAC CTCCTTGGAG TACAGCCTCA GGGTCTACTG CCTCGAGGAC 
ACGCCCGTGG CCCTGAAGGA GGTGCTAGAG CTGGAGCGGA CGCTGGGGGG CTACCTGGTG 
GAGGAGCCCA AGCCCCTGCT GTTCAAGGAC AGTTACCACA ACCTGCGCCT CTCCCTGCAC 
GACGTCCCCC ATGCCCACTG GAGGAGCAAG CTGCTGGCCA AGTACCAGGA GATCCCCTTC 
TGTCACATCT GGAGTGGCAG CCAGAAGGCC CTGCACTGCA CGTTCACCCT GGAGCGGCAC 
AGCCTGGCCA CCACCGAGCT CTCCTGCAAG ATCTGCGTGC GGCAGGTGGA AGGGGAGGGC 
CAGATAGTCC AGCTGCACAC CACGCTGGCC GAGACTCCTG CTGGCTCCCT GGACGCCCTC 
TGCTCTGCGC CCGGCAGCGC CGTCACCACC CAGCTGGGAC CCTATGCCTT CAAGATCCCG 
CTGTCCATCC GCCAAAAGAT ATGCCACAGC CTCGACGCCC CCAGCTCGCG GGGCAGTGAC 
TGGCGGCTGC TGGCGCAGAA GCTCTCCATG GACCGGTACC TGAATTACTT TGCCACCAAA 
GCCAGCCCCA CGGGCGTGAT CCTGGACCTC TGGGAAGCTC TGCAGCAGGA CGACGGGGAC 
CTCAACAGCC TGGCGAGTGC CTTGGAGGAG ATGGGCAAGA GCGAGATGTT GGTGGCCATG 
GCCACTGACG GGGACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005325996.1
CDS	76..2916
Translation	MWARSGTPGALLLALLLCWDPRLSQAGPDPGSQVLPDSFPSAPAEPLPHFLQEPQDAYLVKSKPVELRCRARPATQVYFKCNGEWVGQKDHATQEGLDEATGLRVREVQIEVSRQQVEELFGLEDYWCQCVAWSSAGTTKSRRAYVRIAYLRKNFDQEPLGKEVPLDHEVLLQCRPPEGVPVAEVEWLRNEDVIDPTQDTNFLITIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQAFQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCMQNKRTLSDPKSHLLEASGDVALYAGLVVAILVVVAVLMAVGAVVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPSNPQLLHPSVPPDLTASAGAYRGPVYALQDAADKIPMTNSPLLDPLPSLKIKVYSSSTTSSGPGRPDGADLLGVTYPPGTYPGDFSRDAHFLHLHSASLGSQQPLGLPRDPGSSVSGTFGCLGGRLSIPGTGVSLLVPNGAIPQGKFYEMYLLINKAESTLPLSEGTQTVLSPAVTCGPTGLLLCRPVILTVPHCAEVTAGDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEARSCHILLDQLGTYAFTGESYSRAACKRLQLAVFAPALCTSLEYSLRVYCLEDTPVALKEVLELERTLGGYLVEEPKPLLFKDSYHNLRLSLHDVPHAHWRSKLLAKYQEIPFCHIWSGSQKALHCTFTLERHSLATTELSCKICVRQVEGEGQIVQLHTTLAETPAGSLDALCSAPGSAVTTQLGPYAFKIPLSIRQKICHSLDAPSSRGSDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEALQQDDGDLNSLASALEEMGKSEMLVAMATDGDC

Target ORF information:

RefSeq Version	XM_005325939.2
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus unc-5 netrin receptor B (Unc5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005325939.2

ORF Insert Sequence:

ATGTGGGCCC GCAGCGGAAC GCCGGGCGCT TTGCTGCTGG CGCTGCTACT CTGCTGGGAC 
CCGAGGCTGA GCCAAGCAGG CCCTGACCCC GGCAGCCAGG TGCTGCCCGA CTCCTTCCCG 
TCGGCGCCGG CCGAGCCGCT GCCCCACTTC CTGCAGGAGC CGCAGGACGC CTACCTGGTG 
AAGAGCAAGC CCGTGGAGCT GCGCTGCCGC GCCCGCCCCG CCACGCAGGT CTACTTCAAG 
TGCAACGGCG AGTGGGTCGG CCAGAAGGAC CACGCCACGC AGGAGGGGCT GGACGAGGCC 
ACCGGCCTGC GGGTGCGCGA GGTGCAGATC GAGGTGTCGC GGCAGCAGGT GGAGGAGCTC 
TTTGGGCTGG AGGATTACTG GTGCCAGTGT GTGGCCTGGA GCTCCGCGGG CACCACCAAG 
AGTCGCCGGG CCTACGTGCG CATCGCCTAC CTGCGCAAGA ACTTCGACCA GGAGCCTCTG 
GGCAAGGAAG TGCCCCTGGA CCACGAGGTT CTCCTGCAGT GCCGCCCCCC AGAGGGGGTG 
CCTGTGGCCG AGGTGGAATG GCTCAGGAAT GAGGATGTCA TCGACCCCAC CCAGGACACC 
AACTTCCTGA TCACCATCGA CCACAACCTC ATCATCCGCC AGGCCCGCCT GTCGGACACG 
GCCAACTACA CCTGCGTGGC CAAGAACATC GTGGCCAAGC GCCGGAGCAC CACGGCCACC 
GTCATCGTCT ACGTGAATGG CGGCTGGTCC AGCTGGGCCG AGTGGTCGCC CTGCTCCAAC 
CGCTGTGGCC GAGGCTGGCA GAAGCGCACC CGGACCTGCA CCAACCCCGC CCCGCTCAAC 
GGAGGTGCCT TCTGCGAGGG CCAGGCCTTC CAGAAAACCG CCTGCACCAC CGTGTGCCCA 
GTGGACGGGG CCTGGACAGA GTGGAGCAAG TGGTCGGCCT GCAGCACCGA GTGTGCCCAC 
TGGCGCAGCC GCGAGTGCAT GGCGCCCCCG CCCCAGAACG GAGGCCGCGA CTGCAGCGGG 
ACCCTGCTGG ACTCCAAGAA CTGCACAGAC GGGCTGTGCA TGCAGAATAA GAGGACTCTA 
AGCGACCCCA AAAGCCACCT CCTGGAGGCC TCGGGAGATG TGGCCCTGTA CGCGGGCCTC 
GTGGTGGCCA TCTTGGTGGT GGTGGCGGTC CTCATGGCCG TGGGCGCCGT GGTGTACCGT 
CGCAACTGTC GCGACTTCGA CACGGACATC ACGGACTCGT CCGCTGCCCT CACTGGGGGC 
TTTCATCCAG TCAACTTCAA GACAGCAAGG CCCAGCAACC CTCAGCTCCT GCACCCCTCG 
GTGCCCCCCG ACCTGACAGC CAGCGCCGGC GCCTACCGCG GGCCTGTGTA TGCCCTGCAG 
GATGCGGCCG ACAAGATCCC CATGACCAAC TCGCCCCTGC TCGACCCCCT GCCCAGCCTC 
AAGATCAAGG TCTACAGCTC TAGCACCACT AGCTCCGGGC CCGGCCGGCC AGACGGGGCC 
GACCTGCTGG GGGTCACCTA CCCGCCGGGC ACCTACCCTG GCGACTTCTC CCGGGACGCA 
CACTTCCTGC ACCTGCACAG CGCCAGCCTG GGCTCCCAGC AGCCCCTGGG CCTGCCCCGG 
GACCCGGGCA GCAGCGTCAG CGGCACCTTC GGTTGCCTGG GCGGGAGGCT CAGCATCCCA 
GGCACAGGGG TCAGCCTGCT GGTGCCCAAT GGAGCCATTC CCCAGGGCAA GTTCTACGAG 
ATGTACCTGC TTATCAACAA GGCGGAAAGC ACCCTCCCGC TGTCAGAAGG GACCCAGACA 
GTACTGAGCC CCGCGGTGAC CTGCGGGCCA ACGGGCCTCC TGCTGTGCCG CCCGGTCATC 
CTCACGGTGC CCCACTGTGC TGAGGTCACT GCTGGCGACT GGATCTTCCA GCTCAAGACC 
CAGGCCCACC AGGGCCACTG GGAGGAGGTG GTGACCCTGG ACGAGGAGAC CCTGAACACC 
CCCTGCTACT GCCAGCTGGA GGCCAGGTCC TGCCACATCC TGCTGGACCA GCTGGGCACC 
TACGCCTTCA CGGGCGAGTC CTACTCCCGC GCGGCCTGCA AGCGGCTGCA GCTGGCCGTC 
TTCGCCCCGG CCCTGTGCAC CTCCTTGGAG TACAGCCTCA GGGTCTACTG CCTCGAGGAC 
ACGCCCGTGG CCCTGAAGGA GGTGCTAGAG CTGGAGCGGA CGCTGGGGGG CTACCTGGTG 
GAGGAGCCCA AGCCCCTGCT GTTCAAGGAC AGTTACCACA ACCTGCGCCT CTCCCTGCAC 
GACGTCCCCC ATGCCCACTG GAGGAGCAAG CTGCTGGCCA AGTACCAGGA GATCCCCTTC 
TGTCACATCT GGAGTGGCAG CCAGAAGGCC CTGCACTGCA CGTTCACCCT GGAGCGGCAC 
AGCCTGGCCA CCACCGAGCT CTCCTGCAAG ATCTGCGTGC GGCAGGTGGA AGGGGAGGGC 
CAGATAGTCC AGCTGCACAC CACGCTGGCC GAGACTCCTG CTGGCTCCCT GGACGCCCTC 
TGCTCTGCGC CCGGCAGCGC CGTCACCACC CAGCTGGGAC CCTATGCCTT CAAGATCCCG 
CTGTCCATCC GCCAAAAGAT ATGCCACAGC CTCGACGCCC CCAGCTCGCG GGGCAGTGAC 
TGGCGGCTGC TGGCGCAGAA GCTCTCCATG GACCGGTACC TGAATTACTT TGCCACCAAA 
GCCAGCCCCA CGGGCGTGAT CCTGGACCTC TGGGAAGCTC TGCAGCAGGA CGACGGGGAC 
CTCAACAGCC TGGCGAGTGC CTTGGAGGAG ATGGGCAAGA GCGAGATGTT GGTGGCCATG 
GCCACTGACG GGGACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc5b ( XM_005325939.3 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005325996.1 Ictidomys tridecemlineatus unc-5 netrin receptor B (Unc5b), mRNA.

Price & Availability↑

CloneID	OIc57109
Clone ID Related Accession (Same CDS sequence)	XM_005325939.2 , XM_005325939.3
Accession Version	XM_005325939.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-02
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	netrin receptor UNC5B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936521.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_005325939.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCC GCAGCGGAAC GCCGGGCGCT TTGCTGCTGG CGCTGCTACT CTGCTGGGAC 
CCGAGGCTGA GCCAAGCAGG CCCTGACCCC GGCAGCCAGG TGCTGCCCGA CTCCTTCCCG 
TCGGCGCCGG CCGAGCCGCT GCCCCACTTC CTGCAGGAGC CGCAGGACGC CTACCTGGTG 
AAGAGCAAGC CCGTGGAGCT GCGCTGCCGC GCCCGCCCCG CCACGCAGGT CTACTTCAAG 
TGCAACGGCG AGTGGGTCGG CCAGAAGGAC CACGCCACGC AGGAGGGGCT GGACGAGGCC 
ACCGGCCTGC GGGTGCGCGA GGTGCAGATC GAGGTGTCGC GGCAGCAGGT GGAGGAGCTC 
TTTGGGCTGG AGGATTACTG GTGCCAGTGT GTGGCCTGGA GCTCCGCGGG CACCACCAAG 
AGTCGCCGGG CCTACGTGCG CATCGCCTAC CTGCGCAAGA ACTTCGACCA GGAGCCTCTG 
GGCAAGGAAG TGCCCCTGGA CCACGAGGTT CTCCTGCAGT GCCGCCCCCC AGAGGGGGTG 
CCTGTGGCCG AGGTGGAATG GCTCAGGAAT GAGGATGTCA TCGACCCCAC CCAGGACACC 
AACTTCCTGA TCACCATCGA CCACAACCTC ATCATCCGCC AGGCCCGCCT GTCGGACACG 
GCCAACTACA CCTGCGTGGC CAAGAACATC GTGGCCAAGC GCCGGAGCAC CACGGCCACC 
GTCATCGTCT ACGTGAATGG CGGCTGGTCC AGCTGGGCCG AGTGGTCGCC CTGCTCCAAC 
CGCTGTGGCC GAGGCTGGCA GAAGCGCACC CGGACCTGCA CCAACCCCGC CCCGCTCAAC 
GGAGGTGCCT TCTGCGAGGG CCAGGCCTTC CAGAAAACCG CCTGCACCAC CGTGTGCCCA 
GTGGACGGGG CCTGGACAGA GTGGAGCAAG TGGTCGGCCT GCAGCACCGA GTGTGCCCAC 
TGGCGCAGCC GCGAGTGCAT GGCGCCCCCG CCCCAGAACG GAGGCCGCGA CTGCAGCGGG 
ACCCTGCTGG ACTCCAAGAA CTGCACAGAC GGGCTGTGCA TGCAGAATAA GAGGACTCTA 
AGCGACCCCA AAAGCCACCT CCTGGAGGCC TCGGGAGATG TGGCCCTGTA CGCGGGCCTC 
GTGGTGGCCA TCTTGGTGGT GGTGGCGGTC CTCATGGCCG TGGGCGCCGT GGTGTACCGT 
CGCAACTGTC GCGACTTCGA CACGGACATC ACGGACTCGT CCGCTGCCCT CACTGGGGGC 
TTTCATCCAG TCAACTTCAA GACAGCAAGG CCCAGCAACC CTCAGCTCCT GCACCCCTCG 
GTGCCCCCCG ACCTGACAGC CAGCGCCGGC GCCTACCGCG GGCCTGTGTA TGCCCTGCAG 
GATGCGGCCG ACAAGATCCC CATGACCAAC TCGCCCCTGC TCGACCCCCT GCCCAGCCTC 
AAGATCAAGG TCTACAGCTC TAGCACCACT AGCTCCGGGC CCGGCCGGCC AGACGGGGCC 
GACCTGCTGG GGGTCACCTA CCCGCCGGGC ACCTACCCTG GCGACTTCTC CCGGGACGCA 
CACTTCCTGC ACCTGCACAG CGCCAGCCTG GGCTCCCAGC AGCCCCTGGG CCTGCCCCGG 
GACCCGGGCA GCAGCGTCAG CGGCACCTTC GGTTGCCTGG GCGGGAGGCT CAGCATCCCA 
GGCACAGGGG TCAGCCTGCT GGTGCCCAAT GGAGCCATTC CCCAGGGCAA GTTCTACGAG 
ATGTACCTGC TTATCAACAA GGCGGAAAGC ACCCTCCCGC TGTCAGAAGG GACCCAGACA 
GTACTGAGCC CCGCGGTGAC CTGCGGGCCA ACGGGCCTCC TGCTGTGCCG CCCGGTCATC 
CTCACGGTGC CCCACTGTGC TGAGGTCACT GCTGGCGACT GGATCTTCCA GCTCAAGACC 
CAGGCCCACC AGGGCCACTG GGAGGAGGTG GTGACCCTGG ACGAGGAGAC CCTGAACACC 
CCCTGCTACT GCCAGCTGGA GGCCAGGTCC TGCCACATCC TGCTGGACCA GCTGGGCACC 
TACGCCTTCA CGGGCGAGTC CTACTCCCGC GCGGCCTGCA AGCGGCTGCA GCTGGCCGTC 
TTCGCCCCGG CCCTGTGCAC CTCCTTGGAG TACAGCCTCA GGGTCTACTG CCTCGAGGAC 
ACGCCCGTGG CCCTGAAGGA GGTGCTAGAG CTGGAGCGGA CGCTGGGGGG CTACCTGGTG 
GAGGAGCCCA AGCCCCTGCT GTTCAAGGAC AGTTACCACA ACCTGCGCCT CTCCCTGCAC 
GACGTCCCCC ATGCCCACTG GAGGAGCAAG CTGCTGGCCA AGTACCAGGA GATCCCCTTC 
TGTCACATCT GGAGTGGCAG CCAGAAGGCC CTGCACTGCA CGTTCACCCT GGAGCGGCAC 
AGCCTGGCCA CCACCGAGCT CTCCTGCAAG ATCTGCGTGC GGCAGGTGGA AGGGGAGGGC 
CAGATAGTCC AGCTGCACAC CACGCTGGCC GAGACTCCTG CTGGCTCCCT GGACGCCCTC 
TGCTCTGCGC CCGGCAGCGC CGTCACCACC CAGCTGGGAC CCTATGCCTT CAAGATCCCG 
CTGTCCATCC GCCAAAAGAT ATGCCACAGC CTCGACGCCC CCAGCTCGCG GGGCAGTGAC 
TGGCGGCTGC TGGCGCAGAA GCTCTCCATG GACCGGTACC TGAATTACTT TGCCACCAAA 
GCCAGCCCCA CGGGCGTGAT CCTGGACCTC TGGGAAGCTC TGCAGCAGGA CGACGGGGAC 
CTCAACAGCC TGGCGAGTGC CTTGGAGGAG ATGGGCAAGA GCGAGATGTT GGTGGCCATG 
GCCACTGACG GGGACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005325996.1
CDS	76..2916
Translation	MWARSGTPGALLLALLLCWDPRLSQAGPDPGSQVLPDSFPSAPAEPLPHFLQEPQDAYLVKSKPVELRCRARPATQVYFKCNGEWVGQKDHATQEGLDEATGLRVREVQIEVSRQQVEELFGLEDYWCQCVAWSSAGTTKSRRAYVRIAYLRKNFDQEPLGKEVPLDHEVLLQCRPPEGVPVAEVEWLRNEDVIDPTQDTNFLITIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQAFQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCMQNKRTLSDPKSHLLEASGDVALYAGLVVAILVVVAVLMAVGAVVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPSNPQLLHPSVPPDLTASAGAYRGPVYALQDAADKIPMTNSPLLDPLPSLKIKVYSSSTTSSGPGRPDGADLLGVTYPPGTYPGDFSRDAHFLHLHSASLGSQQPLGLPRDPGSSVSGTFGCLGGRLSIPGTGVSLLVPNGAIPQGKFYEMYLLINKAESTLPLSEGTQTVLSPAVTCGPTGLLLCRPVILTVPHCAEVTAGDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEARSCHILLDQLGTYAFTGESYSRAACKRLQLAVFAPALCTSLEYSLRVYCLEDTPVALKEVLELERTLGGYLVEEPKPLLFKDSYHNLRLSLHDVPHAHWRSKLLAKYQEIPFCHIWSGSQKALHCTFTLERHSLATTELSCKICVRQVEGEGQIVQLHTTLAETPAGSLDALCSAPGSAVTTQLGPYAFKIPLSIRQKICHSLDAPSSRGSDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEALQQDDGDLNSLASALEEMGKSEMLVAMATDGDC

Target ORF information:

RefSeq Version	XM_005325939.3
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus unc-5 netrin receptor B (Unc5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005325939.3

ORF Insert Sequence:

ATGTGGGCCC GCAGCGGAAC GCCGGGCGCT TTGCTGCTGG CGCTGCTACT CTGCTGGGAC 
CCGAGGCTGA GCCAAGCAGG CCCTGACCCC GGCAGCCAGG TGCTGCCCGA CTCCTTCCCG 
TCGGCGCCGG CCGAGCCGCT GCCCCACTTC CTGCAGGAGC CGCAGGACGC CTACCTGGTG 
AAGAGCAAGC CCGTGGAGCT GCGCTGCCGC GCCCGCCCCG CCACGCAGGT CTACTTCAAG 
TGCAACGGCG AGTGGGTCGG CCAGAAGGAC CACGCCACGC AGGAGGGGCT GGACGAGGCC 
ACCGGCCTGC GGGTGCGCGA GGTGCAGATC GAGGTGTCGC GGCAGCAGGT GGAGGAGCTC 
TTTGGGCTGG AGGATTACTG GTGCCAGTGT GTGGCCTGGA GCTCCGCGGG CACCACCAAG 
AGTCGCCGGG CCTACGTGCG CATCGCCTAC CTGCGCAAGA ACTTCGACCA GGAGCCTCTG 
GGCAAGGAAG TGCCCCTGGA CCACGAGGTT CTCCTGCAGT GCCGCCCCCC AGAGGGGGTG 
CCTGTGGCCG AGGTGGAATG GCTCAGGAAT GAGGATGTCA TCGACCCCAC CCAGGACACC 
AACTTCCTGA TCACCATCGA CCACAACCTC ATCATCCGCC AGGCCCGCCT GTCGGACACG 
GCCAACTACA CCTGCGTGGC CAAGAACATC GTGGCCAAGC GCCGGAGCAC CACGGCCACC 
GTCATCGTCT ACGTGAATGG CGGCTGGTCC AGCTGGGCCG AGTGGTCGCC CTGCTCCAAC 
CGCTGTGGCC GAGGCTGGCA GAAGCGCACC CGGACCTGCA CCAACCCCGC CCCGCTCAAC 
GGAGGTGCCT TCTGCGAGGG CCAGGCCTTC CAGAAAACCG CCTGCACCAC CGTGTGCCCA 
GTGGACGGGG CCTGGACAGA GTGGAGCAAG TGGTCGGCCT GCAGCACCGA GTGTGCCCAC 
TGGCGCAGCC GCGAGTGCAT GGCGCCCCCG CCCCAGAACG GAGGCCGCGA CTGCAGCGGG 
ACCCTGCTGG ACTCCAAGAA CTGCACAGAC GGGCTGTGCA TGCAGAATAA GAGGACTCTA 
AGCGACCCCA AAAGCCACCT CCTGGAGGCC TCGGGAGATG TGGCCCTGTA CGCGGGCCTC 
GTGGTGGCCA TCTTGGTGGT GGTGGCGGTC CTCATGGCCG TGGGCGCCGT GGTGTACCGT 
CGCAACTGTC GCGACTTCGA CACGGACATC ACGGACTCGT CCGCTGCCCT CACTGGGGGC 
TTTCATCCAG TCAACTTCAA GACAGCAAGG CCCAGCAACC CTCAGCTCCT GCACCCCTCG 
GTGCCCCCCG ACCTGACAGC CAGCGCCGGC GCCTACCGCG GGCCTGTGTA TGCCCTGCAG 
GATGCGGCCG ACAAGATCCC CATGACCAAC TCGCCCCTGC TCGACCCCCT GCCCAGCCTC 
AAGATCAAGG TCTACAGCTC TAGCACCACT AGCTCCGGGC CCGGCCGGCC AGACGGGGCC 
GACCTGCTGG GGGTCACCTA CCCGCCGGGC ACCTACCCTG GCGACTTCTC CCGGGACGCA 
CACTTCCTGC ACCTGCACAG CGCCAGCCTG GGCTCCCAGC AGCCCCTGGG CCTGCCCCGG 
GACCCGGGCA GCAGCGTCAG CGGCACCTTC GGTTGCCTGG GCGGGAGGCT CAGCATCCCA 
GGCACAGGGG TCAGCCTGCT GGTGCCCAAT GGAGCCATTC CCCAGGGCAA GTTCTACGAG 
ATGTACCTGC TTATCAACAA GGCGGAAAGC ACCCTCCCGC TGTCAGAAGG GACCCAGACA 
GTACTGAGCC CCGCGGTGAC CTGCGGGCCA ACGGGCCTCC TGCTGTGCCG CCCGGTCATC 
CTCACGGTGC CCCACTGTGC TGAGGTCACT GCTGGCGACT GGATCTTCCA GCTCAAGACC 
CAGGCCCACC AGGGCCACTG GGAGGAGGTG GTGACCCTGG ACGAGGAGAC CCTGAACACC 
CCCTGCTACT GCCAGCTGGA GGCCAGGTCC TGCCACATCC TGCTGGACCA GCTGGGCACC 
TACGCCTTCA CGGGCGAGTC CTACTCCCGC GCGGCCTGCA AGCGGCTGCA GCTGGCCGTC 
TTCGCCCCGG CCCTGTGCAC CTCCTTGGAG TACAGCCTCA GGGTCTACTG CCTCGAGGAC 
ACGCCCGTGG CCCTGAAGGA GGTGCTAGAG CTGGAGCGGA CGCTGGGGGG CTACCTGGTG 
GAGGAGCCCA AGCCCCTGCT GTTCAAGGAC AGTTACCACA ACCTGCGCCT CTCCCTGCAC 
GACGTCCCCC ATGCCCACTG GAGGAGCAAG CTGCTGGCCA AGTACCAGGA GATCCCCTTC 
TGTCACATCT GGAGTGGCAG CCAGAAGGCC CTGCACTGCA CGTTCACCCT GGAGCGGCAC 
AGCCTGGCCA CCACCGAGCT CTCCTGCAAG ATCTGCGTGC GGCAGGTGGA AGGGGAGGGC 
CAGATAGTCC AGCTGCACAC CACGCTGGCC GAGACTCCTG CTGGCTCCCT GGACGCCCTC 
TGCTCTGCGC CCGGCAGCGC CGTCACCACC CAGCTGGGAC CCTATGCCTT CAAGATCCCG 
CTGTCCATCC GCCAAAAGAT ATGCCACAGC CTCGACGCCC CCAGCTCGCG GGGCAGTGAC 
TGGCGGCTGC TGGCGCAGAA GCTCTCCATG GACCGGTACC TGAATTACTT TGCCACCAAA 
GCCAGCCCCA CGGGCGTGAT CCTGGACCTC TGGGAAGCTC TGCAGCAGGA CGACGGGGAC 
CTCAACAGCC TGGCGAGTGC CTTGGAGGAG ATGGGCAAGA GCGAGATGTT GGTGGCCATG 
GCCACTGACG GGGACTGCTG A