gbe1 cDNA ORF clone, Ictalurus punctatus(channel catfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gbe1
Entrez Gene ID	108277888
Full Name	1,4-alpha-glucan branching enzyme 1
Gene Type	protein-coding
Organism	Ictalurus punctatus(channel catfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017490921.1	XP_017346410.1	1,4-alpha-glucan-branching enzyme

Pathways from BioSystems
Gene Ontology

The following gbe1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gbe1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc12878	XM_017490921.1 Latest version!	Ictalurus punctatus glucan (1,4-alpha-), branching enzyme 1 (gbe1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gbe1 ( XM_017490921.1 ) cDNA ORF clone, Ictalurus punctatus(channel catfish) -> XP_017346410.1 Ictalurus punctatus glucan (1,4-alpha-), branching enzyme 1 (gbe1), mRNA.

Price & Availability↑

CloneID	OIc12878
Clone ID Related Accession (Same CDS sequence)	XM_017490921.1
Accession Version	XM_017490921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-05
Organism	Ictalurus punctatus(channel catfish)
Product	1,4-alpha-glucan-branching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030432.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictalurus punctatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATA TAGAAACGCA GTTTTCTATT CCCGACTTCA GCACCTATCT ACAAATGGAC 
CCTTACCTCA AACCATATGA AAAGGACTTT AAAAGGAGAT ATGGTTTATT TCAGAAGCAG 
TTGATGCTCC TGGAGGAGGC TGAAGGAGGC TTTGATCAGT TCACACGCAG CTATAAGACC 
TTCGGAGCAC ATCGCCTGCC GGACAACAGC CTCGTCTTTA AAGAGTGGGC TCCAGCTGCT 
GAGGCTCTGT TCCTCACTGG AGACTTCAAT GGCTGGGATA AATTTTCTCA CCCATATAAA 
AAGATGGACT TTGGGAAATG GGAGCTGCAC ATACCACCTA AGGAGGACAA AACACCAGCT 
GTTGCTCACA ATTCCAAGCT AAAGGTGGTG CTTCACACGA AAGAAGGAGA GCGACTGTAC 
CGCATTTCAC CTTGGGCGAC GTATGTTACC AAACCTGACT TGTTGGTTGT GTACGACTGG 
GTGTACTGGG ATCCTCCTCA TCCTTATGTG CATAAACATC CTCGGCCACG GAAGCCCCGC 
AGTTTACGGA TCTATGAGTC ACACGTTGGC ATCGCGTCTC CAGAGGGCAA AGTGGCATCT 
TACAGCAACT TCACACACAA CGTACTGCCT CGAATAAAAG ACCTTGGTTA TAACTGTATT 
CAGCTGATGG CTATCATGGA ACATGCCTAT TATGCTAGCT TTGGATACCA AGTCACAAGT 
TTCTTCGCAG CATCCAGTCG ATTCGGCACT CCGGACGAGC TGAAGGAGCT TATCGATGTG 
GCTCACTCTA TGGGCATCAT GGTGCTTTTA GATGTGGTCC ACAGCCACGC CTCCAAGAAC 
ACAGAGGATG GCTTGAACCG CTTTGATGGC TCTGATTCAT GCTTTTTCCA CAGCGGAGAA 
CGGGGAGATC ATCCTCTCTG GGACAGCCGA CTGTTCAACT ATTCCAGCTG GGAGGTGCTG 
CGATTTCTCT TGTCCAACCT GCGCTGGTGG ATGGAGGAGT ATCGTTTTGA TGGCTTTAGG 
TTCGATGGGA TTACCTCCAT GCTCTATCAC CATCATGGTG TCAGTTCAGG ATTTTCAGGC 
TCGTATTCAG AGTACTTTGG GCTCCAAGTT GACGAGGACT CGCTAGTCTA CCTCATGCTA 
GCCAACCATA TTCTACACAC CCTGTATGAG GACTGCATAA CTGTGGCTGA GGATGTGTCC 
GGTATGCCCA TGCTATGTCG ACCTGTGCAG GAGGGAGGAT GTGGCTTTGA CTACAGGTTG 
GCCATGGCTA TTCCTGACAA GTGGATCCAG ATCTTGAAGG AATTCAAGGA TGAAGACTGG 
AACATCGACA CCATCGTGCA CACTCTGACC AACAGGCGCT ATGGAGAGAA ATGCATTGCG 
TATGCCGAGA GCCATGACCA GGCTCTGGTA GGAGACAAGA CCCTGGCCTT CTGGCTGATG 
GATAAAGACA TGTACACCAA CATGAGCACC TTAATTCCCA TGAACCCCGT CATTGACCGG 
GGCATTCAAC TGCATAAGAT GATCCGGCTC CTGACACATG CCCTGGGAGG GGAGGGCTAC 
CTTAACTTCA TGGGTAATGA ATTTGGTCAT CCGGAGTGGC TGGACTTCCC ACGTGTTGGG 
AATAATGAGA GCTACCACTA TGCACGTCGG CAGTTTAACC TGGTGGACAC AGATCACCTG 
CGCTACTGCC AACTGTACAT CTTCGACCGG GACATGAACC GCACAGAGGA CAAGTATGGC 
TGGCTCGCTG CACCACCGGC ACATGTCAGC ACAAAGCATG AGGGTGACAA GGTGATCGTC 
TTTGAGAGAT CCAATGTCAT TTTCATCTTC AACTTCCACC CCACCAACAG CTACAGCGAC 
TACAGAGTGG GAGCGGGAGC ACCGGGAAAA TATAAGATCA AGCTAGACTC TGATGAGGTT 
CAGTATGGAG GCCATGGCCG ACTGGACCAC AACACGGAAT TCTTCACTGA GCCTGTTTCT 
TATAATGAGC GTCCAAACTC TATGCAGGTT TACATTCCCT GCAGAACGGC TGTTGTTCTA 
GCCAATGAAG AAATCGATTA CTGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017346410.1
CDS	408..2477
Translation	MADIETQFSIPDFSTYLQMDPYLKPYEKDFKRRYGLFQKQLMLLEEAEGGFDQFTRSYKTFGAHRLPDNSLVFKEWAPAAEALFLTGDFNGWDKFSHPYKKMDFGKWELHIPPKEDKTPAVAHNSKLKVVLHTKEGERLYRISPWATYVTKPDLLVVYDWVYWDPPHPYVHKHPRPRKPRSLRIYESHVGIASPEGKVASYSNFTHNVLPRIKDLGYNCIQLMAIMEHAYYASFGYQVTSFFAASSRFGTPDELKELIDVAHSMGIMVLLDVVHSHASKNTEDGLNRFDGSDSCFFHSGERGDHPLWDSRLFNYSSWEVLRFLLSNLRWWMEEYRFDGFRFDGITSMLYHHHGVSSGFSGSYSEYFGLQVDEDSLVYLMLANHILHTLYEDCITVAEDVSGMPMLCRPVQEGGCGFDYRLAMAIPDKWIQILKEFKDEDWNIDTIVHTLTNRRYGEKCIAYAESHDQALVGDKTLAFWLMDKDMYTNMSTLIPMNPVIDRGIQLHKMIRLLTHALGGEGYLNFMGNEFGHPEWLDFPRVGNNESYHYARRQFNLVDTDHLRYCQLYIFDRDMNRTEDKYGWLAAPPAHVSTKHEGDKVIVFERSNVIFIFNFHPTNSYSDYRVGAGAPGKYKIKLDSDEVQYGGHGRLDHNTEFFTEPVSYNERPNSMQVYIPCRTAVVLANEEIDYCY

Target ORF information:

RefSeq Version	XM_017490921.1
Organism	Ictalurus punctatus(channel catfish)
Definition	Ictalurus punctatus glucan (1,4-alpha-), branching enzyme 1 (gbe1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017490921.1

ORF Insert Sequence:

ATGGCAGATA TAGAAACGCA GTTTTCTATT CCCGACTTCA GCACCTATCT ACAAATGGAC 
CCTTACCTCA AACCATATGA AAAGGACTTT AAAAGGAGAT ATGGTTTATT TCAGAAGCAG 
TTGATGCTCC TGGAGGAGGC TGAAGGAGGC TTTGATCAGT TCACACGCAG CTATAAGACC 
TTCGGAGCAC ATCGCCTGCC GGACAACAGC CTCGTCTTTA AAGAGTGGGC TCCAGCTGCT 
GAGGCTCTGT TCCTCACTGG AGACTTCAAT GGCTGGGATA AATTTTCTCA CCCATATAAA 
AAGATGGACT TTGGGAAATG GGAGCTGCAC ATACCACCTA AGGAGGACAA AACACCAGCT 
GTTGCTCACA ATTCCAAGCT AAAGGTGGTG CTTCACACGA AAGAAGGAGA GCGACTGTAC 
CGCATTTCAC CTTGGGCGAC GTATGTTACC AAACCTGACT TGTTGGTTGT GTACGACTGG 
GTGTACTGGG ATCCTCCTCA TCCTTATGTG CATAAACATC CTCGGCCACG GAAGCCCCGC 
AGTTTACGGA TCTATGAGTC ACACGTTGGC ATCGCGTCTC CAGAGGGCAA AGTGGCATCT 
TACAGCAACT TCACACACAA CGTACTGCCT CGAATAAAAG ACCTTGGTTA TAACTGTATT 
CAGCTGATGG CTATCATGGA ACATGCCTAT TATGCTAGCT TTGGATACCA AGTCACAAGT 
TTCTTCGCAG CATCCAGTCG ATTCGGCACT CCGGACGAGC TGAAGGAGCT TATCGATGTG 
GCTCACTCTA TGGGCATCAT GGTGCTTTTA GATGTGGTCC ACAGCCACGC CTCCAAGAAC 
ACAGAGGATG GCTTGAACCG CTTTGATGGC TCTGATTCAT GCTTTTTCCA CAGCGGAGAA 
CGGGGAGATC ATCCTCTCTG GGACAGCCGA CTGTTCAACT ATTCCAGCTG GGAGGTGCTG 
CGATTTCTCT TGTCCAACCT GCGCTGGTGG ATGGAGGAGT ATCGTTTTGA TGGCTTTAGG 
TTCGATGGGA TTACCTCCAT GCTCTATCAC CATCATGGTG TCAGTTCAGG ATTTTCAGGC 
TCGTATTCAG AGTACTTTGG GCTCCAAGTT GACGAGGACT CGCTAGTCTA CCTCATGCTA 
GCCAACCATA TTCTACACAC CCTGTATGAG GACTGCATAA CTGTGGCTGA GGATGTGTCC 
GGTATGCCCA TGCTATGTCG ACCTGTGCAG GAGGGAGGAT GTGGCTTTGA CTACAGGTTG 
GCCATGGCTA TTCCTGACAA GTGGATCCAG ATCTTGAAGG AATTCAAGGA TGAAGACTGG 
AACATCGACA CCATCGTGCA CACTCTGACC AACAGGCGCT ATGGAGAGAA ATGCATTGCG 
TATGCCGAGA GCCATGACCA GGCTCTGGTA GGAGACAAGA CCCTGGCCTT CTGGCTGATG 
GATAAAGACA TGTACACCAA CATGAGCACC TTAATTCCCA TGAACCCCGT CATTGACCGG 
GGCATTCAAC TGCATAAGAT GATCCGGCTC CTGACACATG CCCTGGGAGG GGAGGGCTAC 
CTTAACTTCA TGGGTAATGA ATTTGGTCAT CCGGAGTGGC TGGACTTCCC ACGTGTTGGG 
AATAATGAGA GCTACCACTA TGCACGTCGG CAGTTTAACC TGGTGGACAC AGATCACCTG 
CGCTACTGCC AACTGTACAT CTTCGACCGG GACATGAACC GCACAGAGGA CAAGTATGGC 
TGGCTCGCTG CACCACCGGC ACATGTCAGC ACAAAGCATG AGGGTGACAA GGTGATCGTC 
TTTGAGAGAT CCAATGTCAT TTTCATCTTC AACTTCCACC CCACCAACAG CTACAGCGAC 
TACAGAGTGG GAGCGGGAGC ACCGGGAAAA TATAAGATCA AGCTAGACTC TGATGAGGTT 
CAGTATGGAG GCCATGGCCG ACTGGACCAC AACACGGAAT TCTTCACTGA GCCTGTTTCT 
TATAATGAGC GTCCAAACTC TATGCAGGTT TACATTCCCT GCAGAACGGC TGTTGTTCTA 
GCCAATGAAG AAATCGATTA CTGCTACTGA