nup85 cDNA ORF clone, Ictalurus punctatus(channel catfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nup85
Entrez Gene ID	108272821
Full Name	nucleoporin 85
Gene Type	protein-coding
Organism	Ictalurus punctatus(channel catfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017481602.1	XP_017337091.1	nuclear pore complex protein Nup85

Pathways from BioSystems
Gene Ontology

The following nup85 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nup85 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc05767	XM_017481602.1 Latest version!	Ictalurus punctatus nucleoporin 85 (nup85), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nup85 ( XM_017481602.1 ) cDNA ORF clone, Ictalurus punctatus(channel catfish) -> XP_017337091.1 Ictalurus punctatus nucleoporin 85 (nup85), mRNA.

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CloneID	OIc05767
Clone ID Related Accession (Same CDS sequence)	XM_017481602.1
Accession Version	XM_017481602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-05
Organism	Ictalurus punctatus(channel catfish)
Product	nuclear pore complex protein Nup85
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictalurus punctatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAT TAGATGTGGA GCCGACAACC ACGCTGATCC CAGGCGCAGG CCTTAAACAG 
AAGCACCTGG GCTTTGAGTG GGGTCCTGGA GACCTGCTGA TGTACGAGAC CAACTTTAAA 
TTACAAGATT CTGGCTCTGG AGGCTCTGGT TCTCCCTTCA TCCACGTGGT GCGCAGAGAT 
GAGGACATCT ACTCTCCCAT CCTGCGCAAG CTCTTCAACG AGTCTCATCA CATCTTCGTC 
GGGCTGCAGA ACATTAAAGA TGACGTTCCA AGCAAGAACA AGAAACTGCA GTTTGTGAGC 
ATCAGTAAGA ACTACAGGTC TGTAATACGA GCCTGTATGG AGGAGCTGCA GCAGGTCGCA 
GTATCGACTA CAGACACACA GTACAGCAAC CAGGTGTCCA TTCTCTTAGC CATTGAACTC 
ATCTGGAATC TGTGTGAGGT GCTTTTCATC GACGCATCAC CAGCCGGCTC TCTGCTGCTG 
CACCTGCTGG ACTGGGTGCG CTTACACAAA GCTGACGTGG ATGAAAAAGC GCGAGAGGTC 
CTGCAGAGCG AAAGCCCCAC ACGTCATCAT GCGTACTGGG ACGTGGTGAT GTGTTTTGTC 
CTGCAGGGCC GAATGGACGA GGCTCGACAG GTTCTGCAGA AACAGGCGTC TCTACAGCCG 
GCGTCCAGAG CCGCGTATCA GCTCGTGGAC AACCTGCTGC ACAAAATGCC CGTATTTAAC 
CCCGGCGGCA CTCAGACCCT CACAGAGTTT GATGTAAAGT GGAGGCACTG GCATGAGGAG 
TGTGACCGCT GTCTCCAAGA CAACTCCTTC GCTAGCAACC GTCACCTGGA AACCATCTGC 
AAGGTCCTGG TTGGTGACGA GGATGCTCTG TTGGAGCAGA AAGTGCTGCT GGGAACCTGG 
TACCATTTAC TGATCAGCCG CCTGCTGTTC TCTCATCCCA CTGTTAAACC CGCTGAGCTG 
CACTACTACG CTCAGTCAAG TATGGACATG TTCCTGGAGT CTCCCAGCGC CTCAGAGCCT 
CTGGACAGCA TCTTACTGGC TGCGTTTGAG TTCGACCTTC ACCAAGTTAT TAAAGACTGC 
AGCATTGCCC TGAATAACTG GTGGTTTGTG GCTCACCTGA CTGACCTGCT GGATCACTGC 
AAACTCCTGC AGTCGCACAA CCTCCATTTT GGCTCTAACC TGCGGGAGTT CCTGGTGCTG 
GAGTACGCCT CTGGACTCTT CTCTCATCAC AGTTTGTGGC AGCTTGCAGT GGATTATTTC 
GATCACTGCC CCGAGTTCGG TCGTGTGTAT CTGGAGCTGC AGATCGAGAG AGTGCCGCTG 
GAGACGGAGC GCAAAGCGCT GAAGGTGCTG AGGATCTGTG AACAGAGACA GATGAATGAG 
CAAGTGCGCA GTATCTGTAA GATCATGGCT AAAAGGGCTC TCAGTAATAA CAGACTGGGT 
TCTGCTCTGT CCTGGAGTAT CAGAGCTAAA GACGCAGCCT TTGCCACACT CATCTCCGAG 
AGGTTTCTGC AGGATTACTG CACACGCGGC TCATTCTCTG ATCTGGATCT GATCGATAAT 
CTTGGACCTG CCATGCTCCT CAGTGATAGA CTCACATTCC TCGGGAAGTA CCGTGAGTTC 
CACCGGCTGT ATGGAGAAAA GAAGTTCGCA GAGGCGGCGA AGCTCCTGCT CTCCCTCATG 
ACAGCAAAGA TCGCTCCACG CAGCTTCTGG ATGACTCTGC TCATAGACGC CCTGCCCTTA 
CTGGAGCAGA AAGAGGTGAT CTTTTCAGTG GATCAGACCC ATGAACTCAT GTTCTGTCTG 
GAAGAGCTGA CCTCTGACAA CAGCGAGCTC AAACTAGAGC ATCTCACACA GGATGATGTC 
GAGTCCACAA AGCTGGAGCT GCTGCGATTG GCTCTGGCGC GTAACCTGGC GATGGCCATC 
GTGAAGGAGG GAACAGTCGA GGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017337091.1
CDS	136..2082
Translation	MEELDVEPTTTLIPGAGLKQKHLGFEWGPGDLLMYETNFKLQDSGSGGSGSPFIHVVRRDEDIYSPILRKLFNESHHIFVGLQNIKDDVPSKNKKLQFVSISKNYRSVIRACMEELQQVAVSTTDTQYSNQVSILLAIELIWNLCEVLFIDASPAGSLLLHLLDWVRLHKADVDEKAREVLQSESPTRHHAYWDVVMCFVLQGRMDEARQVLQKQASLQPASRAAYQLVDNLLHKMPVFNPGGTQTLTEFDVKWRHWHEECDRCLQDNSFASNRHLETICKVLVGDEDALLEQKVLLGTWYHLLISRLLFSHPTVKPAELHYYAQSSMDMFLESPSASEPLDSILLAAFEFDLHQVIKDCSIALNNWWFVAHLTDLLDHCKLLQSHNLHFGSNLREFLVLEYASGLFSHHSLWQLAVDYFDHCPEFGRVYLELQIERVPLETERKALKVLRICEQRQMNEQVRSICKIMAKRALSNNRLGSALSWSIRAKDAAFATLISERFLQDYCTRGSFSDLDLIDNLGPAMLLSDRLTFLGKYREFHRLYGEKKFAEAAKLLLSLMTAKIAPRSFWMTLLIDALPLLEQKEVIFSVDQTHELMFCLEELTSDNSELKLEHLTQDDVESTKLELLRLALARNLAMAIVKEGTVEA

Target ORF information:

RefSeq Version	XM_017481602.1
Organism	Ictalurus punctatus(channel catfish)
Definition	Ictalurus punctatus nucleoporin 85 (nup85), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017481602.1

ORF Insert Sequence:

ATGGAGGAAT TAGATGTGGA GCCGACAACC ACGCTGATCC CAGGCGCAGG CCTTAAACAG 
AAGCACCTGG GCTTTGAGTG GGGTCCTGGA GACCTGCTGA TGTACGAGAC CAACTTTAAA 
TTACAAGATT CTGGCTCTGG AGGCTCTGGT TCTCCCTTCA TCCACGTGGT GCGCAGAGAT 
GAGGACATCT ACTCTCCCAT CCTGCGCAAG CTCTTCAACG AGTCTCATCA CATCTTCGTC 
GGGCTGCAGA ACATTAAAGA TGACGTTCCA AGCAAGAACA AGAAACTGCA GTTTGTGAGC 
ATCAGTAAGA ACTACAGGTC TGTAATACGA GCCTGTATGG AGGAGCTGCA GCAGGTCGCA 
GTATCGACTA CAGACACACA GTACAGCAAC CAGGTGTCCA TTCTCTTAGC CATTGAACTC 
ATCTGGAATC TGTGTGAGGT GCTTTTCATC GACGCATCAC CAGCCGGCTC TCTGCTGCTG 
CACCTGCTGG ACTGGGTGCG CTTACACAAA GCTGACGTGG ATGAAAAAGC GCGAGAGGTC 
CTGCAGAGCG AAAGCCCCAC ACGTCATCAT GCGTACTGGG ACGTGGTGAT GTGTTTTGTC 
CTGCAGGGCC GAATGGACGA GGCTCGACAG GTTCTGCAGA AACAGGCGTC TCTACAGCCG 
GCGTCCAGAG CCGCGTATCA GCTCGTGGAC AACCTGCTGC ACAAAATGCC CGTATTTAAC 
CCCGGCGGCA CTCAGACCCT CACAGAGTTT GATGTAAAGT GGAGGCACTG GCATGAGGAG 
TGTGACCGCT GTCTCCAAGA CAACTCCTTC GCTAGCAACC GTCACCTGGA AACCATCTGC 
AAGGTCCTGG TTGGTGACGA GGATGCTCTG TTGGAGCAGA AAGTGCTGCT GGGAACCTGG 
TACCATTTAC TGATCAGCCG CCTGCTGTTC TCTCATCCCA CTGTTAAACC CGCTGAGCTG 
CACTACTACG CTCAGTCAAG TATGGACATG TTCCTGGAGT CTCCCAGCGC CTCAGAGCCT 
CTGGACAGCA TCTTACTGGC TGCGTTTGAG TTCGACCTTC ACCAAGTTAT TAAAGACTGC 
AGCATTGCCC TGAATAACTG GTGGTTTGTG GCTCACCTGA CTGACCTGCT GGATCACTGC 
AAACTCCTGC AGTCGCACAA CCTCCATTTT GGCTCTAACC TGCGGGAGTT CCTGGTGCTG 
GAGTACGCCT CTGGACTCTT CTCTCATCAC AGTTTGTGGC AGCTTGCAGT GGATTATTTC 
GATCACTGCC CCGAGTTCGG TCGTGTGTAT CTGGAGCTGC AGATCGAGAG AGTGCCGCTG 
GAGACGGAGC GCAAAGCGCT GAAGGTGCTG AGGATCTGTG AACAGAGACA GATGAATGAG 
CAAGTGCGCA GTATCTGTAA GATCATGGCT AAAAGGGCTC TCAGTAATAA CAGACTGGGT 
TCTGCTCTGT CCTGGAGTAT CAGAGCTAAA GACGCAGCCT TTGCCACACT CATCTCCGAG 
AGGTTTCTGC AGGATTACTG CACACGCGGC TCATTCTCTG ATCTGGATCT GATCGATAAT 
CTTGGACCTG CCATGCTCCT CAGTGATAGA CTCACATTCC TCGGGAAGTA CCGTGAGTTC 
CACCGGCTGT ATGGAGAAAA GAAGTTCGCA GAGGCGGCGA AGCTCCTGCT CTCCCTCATG 
ACAGCAAAGA TCGCTCCACG CAGCTTCTGG ATGACTCTGC TCATAGACGC CCTGCCCTTA 
CTGGAGCAGA AAGAGGTGAT CTTTTCAGTG GATCAGACCC ATGAACTCAT GTTCTGTCTG 
GAAGAGCTGA CCTCTGACAA CAGCGAGCTC AAACTAGAGC ATCTCACACA GGATGATGTC 
GAGTCCACAA AGCTGGAGCT GCTGCGATTG GCTCTGGCGC GTAACCTGGC GATGGCCATC 
GTGAAGGAGG GAACAGTCGA GGCATAA