143b1 cDNA ORF clone, Ictalurus punctatus(channel catfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	143b1
Entrez Gene ID	100528489
Full Name	14-3-3 protein beta/alpha-1
Gene Type	protein-coding
Organism	Ictalurus punctatus(channel catfish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001200759.1	NP_001187688.1	14-3-3 protein beta/alpha-1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).
Chen F, Lee Y, Jiang Y, Wang S, Peatman E, Abernathy J, Liu H, Liu S, Kucuktas H, Ke C, Liu Z
PloS one5(7)e11546(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following 143b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the 143b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc39697	NM_001200759.1 Latest version!	Ictalurus punctatus 14-3-3 protein beta/alpha-1 (143b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

143b1 ( NM_001200759.1 ) cDNA ORF clone, Ictalurus punctatus(channel catfish) -> NP_001187688.1 Ictalurus punctatus 14-3-3 protein beta/alpha-1 (143b1), mRNA.

Price & Availability↑

CloneID	OIc39697
Clone ID Related Accession (Same CDS sequence)	NM_001200759.1
Accession Version	NM_001200759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-04
Organism	Ictalurus punctatus(channel catfish)
Product	14-3-3 protein beta/alpha-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GU589037.1. ##Evidence-Data-START## Transcript exon combination :: GU589037.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00774075, SAMN01906360 [ECO:0000348] ##Evidence-Data-END##

ATGGACAAGA GCGACCTGGT GCAGAAAGCC AAGCTCGCCG AACAGGCGGA GCGCTACGAT 
GACATGGCCG CTGCCATGAA GGCGGTGACT GAGGGCGGCG ACGAGCTGTC CAACGAAGAG 
CGTAACCTGC TGTCTGTGGC CTACAAGAAC GTGGTTGGTG CACGCCGCTC TTCCTGGCGT 
GTCATCTCCA GCATCGAGCA GAAGACCGAG TCCAACGAGA AGAAGCAGCT GATGGCACGC 
GAGTACCGCG AGAAGATCGA GAGCGAGCTG AAGGAAATCT GCAATGATGT TCTGGGACTC 
CTGGATAAGT ATCTCATTCC CAATGCCTCT CCGGCTGAGG GCAAGGTGTT CTACCTGAAG 
ATGAAAGGCG ATTATTATAG ATACCTGTCT GAGGTTGCAT CTGGGGACTC CAAGAGCTCT 
ACAGTCGATA ACTCTCAGAA GGCCTACCAG GATGCGTTTG AGATCAGCAA GAAAGACATG 
CAGCCCACAC ACCCCATCCG GCTTGGTCTG GCTCTCAACT TCTCTGTCTT CTATTATGAG 
ATCCTGAACT CACCCGAGAA GGCCTGCAAC CTTGCCAAAA CGGCCTTCGA TGAAGGCATT 
GCTGAGCTTG ACACCTTAAA TGAGGATTCC TACAAAGACA GCACCTCGAT CATGCAGTTA 
CTAAGGGACA ATCTTACCCT GTGGACGTCG GAAAACCAGG GTGACGATGC AGATGCAGTC 
GAGGGAGAGA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001187688.1
CDS	81..815
Translation	MDKSDLVQKAKLAEQAERYDDMAAAMKAVTEGGDELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTESNEKKQLMAREYREKIESELKEICNDVLGLLDKYLIPNASPAEGKVFYLKMKGDYYRYLSEVASGDSKSSTVDNSQKAYQDAFEISKKDMQPTHPIRLGLALNFSVFYYEILNSPEKACNLAKTAFDEGIAELDTLNEDSYKDSTSIMQLLRDNLTLWTSENQGDDADAVEGEN

Target ORF information:

RefSeq Version	NM_001200759.1
Organism	Ictalurus punctatus(channel catfish)
Definition	Ictalurus punctatus 14-3-3 protein beta/alpha-1 (143b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001200759.1

ORF Insert Sequence:

ATGGACAAGA GCGACCTGGT GCAGAAAGCC AAGCTCGCCG AACAGGCGGA GCGCTACGAT 
GACATGGCCG CTGCCATGAA GGCGGTGACT GAGGGCGGCG ACGAGCTGTC CAACGAAGAG 
CGTAACCTGC TGTCTGTGGC CTACAAGAAC GTGGTTGGTG CACGCCGCTC TTCCTGGCGT 
GTCATCTCCA GCATCGAGCA GAAGACCGAG TCCAACGAGA AGAAGCAGCT GATGGCACGC 
GAGTACCGCG AGAAGATCGA GAGCGAGCTG AAGGAAATCT GCAATGATGT TCTGGGACTC 
CTGGATAAGT ATCTCATTCC CAATGCCTCT CCGGCTGAGG GCAAGGTGTT CTACCTGAAG 
ATGAAAGGCG ATTATTATAG ATACCTGTCT GAGGTTGCAT CTGGGGACTC CAAGAGCTCT 
ACAGTCGATA ACTCTCAGAA GGCCTACCAG GATGCGTTTG AGATCAGCAA GAAAGACATG 
CAGCCCACAC ACCCCATCCG GCTTGGTCTG GCTCTCAACT TCTCTGTCTT CTATTATGAG 
ATCCTGAACT CACCCGAGAA GGCCTGCAAC CTTGCCAAAA CGGCCTTCGA TGAAGGCATT 
GCTGAGCTTG ACACCTTAAA TGAGGATTCC TACAAAGACA GCACCTCGAT CATGCAGTTA 
CTAAGGGACA ATCTTACCCT GTGGACGTCG GAAAACCAGG GTGACGATGC AGATGCAGTC 
GAGGGAGAGA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).
PloS one5(7)e11546(2010 Jul)
Chen F,Lee Y,Jiang Y,Wang S,Peatman E,Abernathy J,Liu H,Liu S,Kucuktas H,Ke C,Liu Z