CACNA1B gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017015118.1	XP_016870607.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
XM_017015120.1	XP_016870609.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
XM_011518991.2	XP_011517293.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
NM_001243812.2	NP_001230741.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform 2
NM_001243812.1	NP_001230741.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform 2
XM_017015115.1	XP_016870604.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
XM_011518993.2	XP_011517295.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
XM_011518992.2	XP_011517294.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
NM_000718.3	NP_000709.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform 1
XM_011518994.2	XP_011517296.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
XM_017015119.1	XP_016870608.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
XM_017015116.1	XP_016870605.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
XM_017015117.1	XP_016870606.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
NM_000718.4	NP_000709.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28838	NM_000718.4 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$929.50-$1301.30 $1859.00
OHu28817	NM_001243812.2 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu56562	XM_011518991.2 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu56563	XM_011518992.2 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu56564	XM_011518993.2 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu56565	XM_011518994.2 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu98898	XM_017015115.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu98899	XM_017015116.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu98900	XM_017015117.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu98901	XM_017015118.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu98902	XM_017015119.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu98903	XM_017015120.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
View Old Accession Versions >>
OHu28817	NM_001243812.1	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu28838	NM_000718.3	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$929.50-$1301.30 $1859.00
Hide Old Accession Versions >>

CACNA1B ( NM_000718.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000709.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28838
Clone ID Related Accession (Same CDS sequence)	NM_000718.3 , NM_000718.4
Accession Version	NM_000718.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-09
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M94172.1, AL772363.15, AL591424.12 and BC033060.1. This sequence is a reference standard in the RefSeqGene project. On Aug 30, 2011 this sequence version replaced NM_000718.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M94172.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC AGGATCCAAA 
GGGAAAGACA TCAATACCAT CAAGTCTCTG AGAGTCCTTC GTGTCCTGCG GCCCCTCAAG 
ACCATCAAAC GGCTGCCCAA GCTCAAGGCT GTGTTTGACT GTGTGGTGAA CTCCCTGAAG 
AATGTCCTCA ACATCTTGAT TGTCTACATG CTCTTCATGT TCATATTTGC CGTCATTGCG 
GTGCAGCTCT TCAAAGGGAA GTTTTTCTAC TGCACAGATG AATCCAAGGA GCTGGAGAGG 
GACTGCAGGG GTCAGTATTT GGATTATGAG AAGGAGGAAG TGGAAGCTCA GCCCAGGCAG 
TGGAAGAAAT ACGACTTTCA CTACGACAAT GTGCTCTGGG CTCTGCTGAC GCTGTTCACA 
GTGTCCACGG GAGAAGGCTG GCCCATGGTG CTGAAACACT CCGTGGATGC CACCTATGAG 
GAGCAGGGTC CAAGCCCTGG GTACCGCATG GAGCTGTCCA TCTTCTACGT GGTCTACTTT 
GTGGTCTTTC CCTTCTTCTT CGTCAACATC TTTGTGGCTT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGGTGAT GTCTGAATGC AGCCTGGAGA AGAACGAGAG GGCTTGCATT 
GACTTCGCCA TCAGCGCCAA ACCCCTGACA CGGTACATGC CCCAAAACCG GCAGTCGTTC 
CAGTATAAGA CGTGGACATT TGTGGTCTCC CCGCCCTTTG AATACTTCAT CATGGCCATG 
ATAGCCCTCA ACACTGTGGT GCTGATGATG AAGTTCTATG ATGCACCCTA TGAGTACGAG 
CTGATGCTGA AATGCCTGAA CATCGTGTTC ACATCCATGT TCTCCATGGA ATGCGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAACTAT TTCAGAGATG CCTGGAATGT CTTTGACTTT 
GTCACTGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA AACGAACAAT 
TTCATCAACC TCAGCTTCCT CCGCCTCTTT CGAGCTGCGC GGCTGATCAA GCTGCTCCGC 
CAGGGCTACA CCATCCGCAT CCTGCTGTGG ACCTTTGTCC AGTCCTTCAA GGCCCTGCCC 
TACGTGTGTC TGCTCATTGC CATGCTGTTC TTCATCTACG CCATCATCGG CATGCAGGTG 
TTTGGGAATA TTGCCCTGGA TGATGACACC AGCATCAACC GCCACAACAA CTTCCGGACG 
TTTTTGCAAG CCCTGATGCT GCTGTTCAGG AGCGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCCT GCCTGAGCAA CCAGGCCTGT GATGAGCAGG CCAATGCCAC CGAGTGTGGA 
AGTGACTTTG CCTACTTCTA CTTCGTCTCC TTCATCTTCC TGTGCTCCTT TCTGATGTTG 
AACCTCTTTG TGGCTGTGAT CATGGACAAT TTTGAGTACC TCACGCGGGA CTCTTCCATC 
CTAGGTCCTC ACCACTTGGA TGAGTTCATC CGGGTCTGGG CTGAATACGA CCCGGCTGCG 
TGTGGGCGCA TCAGTTACAA TGACATGTTT GAGATGCTGA AACACATGTC CCCGCCTCTG 
GGGCTGGGGA AGAAATGCCC TGCTCGAGTT GCTTACAAGC GCCTGGTTCG CATGAACATG 
CCCATCTCCA ACGAGGACAT GACTGTTCAC TTCACGTCCA CGCTGATGGC CCTCATCCGG 
ACGGCACTGG AGATCAAGCT GGCCCCAGCT GGGACAAAGC AGCATCAGTG TGACGCGGAG 
TTGAGGAAGG AGATTTCCGT TGTGTGGGCC AATCTGCCCC AGAAGACTTT GGACTTGCTG 
GTACCACCCC ATAAGCCTGA TGAGATGACA GTGGGGAAGG TTTATGCAGC TCTGATGATA 
TTCGACTTCT ACAAGCAGAA CAAAACCACC AGAGACCAGA TGCAGCAGGC TCCTGGAGGC 
CTCTCCCAGA TGGGTCCTGT GTCCCTGTTC CACCCTCTGA AGGCCACCCT GGAGCAGACA 
CAGCCGGCTG TGCTCCGAGG AGCCCGGGTT TTCCTTCGAC AGAAGAGTTC CACCTCCCTC 
AGCAATGGCG GGGCCATACA AAACCAAGAG AGTGGCATCA AAGAGTCTGT CTCCTGGGGC 
ACTCAAAGGA CCCAGGATGC ACCCCATGAG GCCAGGCCAC CCCTGGAGCG TGGCCACTCC 
ACAGAGATCC CTGTGGGGCG GTCAGGAGCA CTGGCTGTGG ACGTTCAGAT GCAGAGCATA 
ACCCGGAGGG GCCCTGATGG GGAGCCCCAG CCTGGGCTGG AGAGCCAGGG TCGAGCGGCC 
TCCATGCCCC GCCTTGCGGC CGAGACTCAG CCCGTCACAG ATGCCAGCCC CATGAAGCGC 
TCCATCTCCA CGCTGGCCCA GCGGCCCCGT GGGACTCATC TTTGCAGCAC CACCCCGGAC 
CGCCCACCCC CTAGCCAGGC GTCGTCGCAC CACCACCACC ACCGCTGCCA CCGCCGCAGG 
GACAGGAAGC AGAGGTCCCT GGAGAAGGGG CCCAGCCTGT CTGCCGATAT GGATGGCGCA 
CCAAGCAGTG CTGTGGGGCC GGGGCTGCCC CCGGGAGAGG GGCCTACAGG CTGCCGGCGG 
GAACGAGAGC GCCGGCAGGA GCGGGGCCGG TCCCAGGAGC GGAGGCAGCC CTCATCCTCC 
TCCTCGGAGA AGCAGCGCTT CTACTCCTGC GACCGCTTTG GGGGCCGTGA GCCCCCGAAG 
CCCAAGCCCT CCCTCAGCAG CCACCCAACG TCGCCAACAG CTGGCCAGGA GCCGGGACCC 
CACCCACAGG GCAGTGGTTC CGTGAATGGG AGCCCCTTGC TGTCAACATC TGGTGCTAGC 
ACCCCCGGCC GCGGTGGGCG GAGGCAGCTC CCCCAGACGC CCCTGACTCC CCGCCCCAGC 
ATCACCTACA AGACGGCCAA CTCCTCACCC ATCCACTTCG CCGGGGCTCA GACCAGCCTC 
CCTGCCTTCT CCCCAGGCCG GCTCAGCCGT GGGCTTTCCG AACACAACGC CCTGCTGCAG 
AGAGACCCCC TCAGCCAGCC CCTGGCCCCT GGCTCTCGAA TTGGCTCTGA CCCTTACCTG 
GGGCAGCGTC TGGACAGTGA GGCCTCTGTC CACGCCCTGC CTGAGGACAC TCTCACTTTC 
GAGGAGGCTG TGGCCACCAA CTCGGGCCGC TCCTCCAGGA CTTCCTACGT GTCCTCCCTG 
ACCTCCCAGT CTCACCCTCT CCGCCGCGTG CCCAACGGTT ACCACTGCAC CCTGGGACTC 
AGCTCGGGTG GCCGAGCACG GCACAGCTAC CACCACCCTG ACCAAGACCA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000709.1
CDS	146..7165
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPIHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPGSRIGSDPYLGQRLDSEASVHALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	NM_000718.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000718.3

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC AGGATCCAAA 
GGGAAAGACA TCAATACCAT CAAGTCTCTG AGAGTCCTTC GTGTCCTGCG GCCCCTCAAG 
ACCATCAAAC GGCTGCCCAA GCTCAAGGCT GTGTTTGACT GTGTGGTGAA CTCCCTGAAG 
AATGTCCTCA ACATCTTGAT TGTCTACATG CTCTTCATGT TCATATTTGC CGTCATTGCG 
GTGCAGCTCT TCAAAGGGAA GTTTTTCTAC TGCACAGATG AATCCAAGGA GCTGGAGAGG 
GACTGCAGGG GTCAGTATTT GGATTATGAG AAGGAGGAAG TGGAAGCTCA GCCCAGGCAG 
TGGAAGAAAT ACGACTTTCA CTACGACAAT GTGCTCTGGG CTCTGCTGAC GCTGTTCACA 
GTGTCCACGG GAGAAGGCTG GCCCATGGTG CTGAAACACT CCGTGGATGC CACCTATGAG 
GAGCAGGGTC CAAGCCCTGG GTACCGCATG GAGCTGTCCA TCTTCTACGT GGTCTACTTT 
GTGGTCTTTC CCTTCTTCTT CGTCAACATC TTTGTGGCTT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGGTGAT GTCTGAATGC AGCCTGGAGA AGAACGAGAG GGCTTGCATT 
GACTTCGCCA TCAGCGCCAA ACCCCTGACA CGGTACATGC CCCAAAACCG GCAGTCGTTC 
CAGTATAAGA CGTGGACATT TGTGGTCTCC CCGCCCTTTG AATACTTCAT CATGGCCATG 
ATAGCCCTCA ACACTGTGGT GCTGATGATG AAGTTCTATG ATGCACCCTA TGAGTACGAG 
CTGATGCTGA AATGCCTGAA CATCGTGTTC ACATCCATGT TCTCCATGGA ATGCGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAACTAT TTCAGAGATG CCTGGAATGT CTTTGACTTT 
GTCACTGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA AACGAACAAT 
TTCATCAACC TCAGCTTCCT CCGCCTCTTT CGAGCTGCGC GGCTGATCAA GCTGCTCCGC 
CAGGGCTACA CCATCCGCAT CCTGCTGTGG ACCTTTGTCC AGTCCTTCAA GGCCCTGCCC 
TACGTGTGTC TGCTCATTGC CATGCTGTTC TTCATCTACG CCATCATCGG CATGCAGGTG 
TTTGGGAATA TTGCCCTGGA TGATGACACC AGCATCAACC GCCACAACAA CTTCCGGACG 
TTTTTGCAAG CCCTGATGCT GCTGTTCAGG AGCGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCCT GCCTGAGCAA CCAGGCCTGT GATGAGCAGG CCAATGCCAC CGAGTGTGGA 
AGTGACTTTG CCTACTTCTA CTTCGTCTCC TTCATCTTCC TGTGCTCCTT TCTGATGTTG 
AACCTCTTTG TGGCTGTGAT CATGGACAAT TTTGAGTACC TCACGCGGGA CTCTTCCATC 
CTAGGTCCTC ACCACTTGGA TGAGTTCATC CGGGTCTGGG CTGAATACGA CCCGGCTGCG 
TGTGGGCGCA TCAGTTACAA TGACATGTTT GAGATGCTGA AACACATGTC CCCGCCTCTG 
GGGCTGGGGA AGAAATGCCC TGCTCGAGTT GCTTACAAGC GCCTGGTTCG CATGAACATG 
CCCATCTCCA ACGAGGACAT GACTGTTCAC TTCACGTCCA CGCTGATGGC CCTCATCCGG 
ACGGCACTGG AGATCAAGCT GGCCCCAGCT GGGACAAAGC AGCATCAGTG TGACGCGGAG 
TTGAGGAAGG AGATTTCCGT TGTGTGGGCC AATCTGCCCC AGAAGACTTT GGACTTGCTG 
GTACCACCCC ATAAGCCTGA TGAGATGACA GTGGGGAAGG TTTATGCAGC TCTGATGATA 
TTCGACTTCT ACAAGCAGAA CAAAACCACC AGAGACCAGA TGCAGCAGGC TCCTGGAGGC 
CTCTCCCAGA TGGGTCCTGT GTCCCTGTTC CACCCTCTGA AGGCCACCCT GGAGCAGACA 
CAGCCGGCTG TGCTCCGAGG AGCCCGGGTT TTCCTTCGAC AGAAGAGTTC CACCTCCCTC 
AGCAATGGCG GGGCCATACA AAACCAAGAG AGTGGCATCA AAGAGTCTGT CTCCTGGGGC 
ACTCAAAGGA CCCAGGATGC ACCCCATGAG GCCAGGCCAC CCCTGGAGCG TGGCCACTCC 
ACAGAGATCC CTGTGGGGCG GTCAGGAGCA CTGGCTGTGG ACGTTCAGAT GCAGAGCATA 
ACCCGGAGGG GCCCTGATGG GGAGCCCCAG CCTGGGCTGG AGAGCCAGGG TCGAGCGGCC 
TCCATGCCCC GCCTTGCGGC CGAGACTCAG CCCGTCACAG ATGCCAGCCC CATGAAGCGC 
TCCATCTCCA CGCTGGCCCA GCGGCCCCGT GGGACTCATC TTTGCAGCAC CACCCCGGAC 
CGCCCACCCC CTAGCCAGGC GTCGTCGCAC CACCACCACC ACCGCTGCCA CCGCCGCAGG 
GACAGGAAGC AGAGGTCCCT GGAGAAGGGG CCCAGCCTGT CTGCCGATAT GGATGGCGCA 
CCAAGCAGTG CTGTGGGGCC GGGGCTGCCC CCGGGAGAGG GGCCTACAGG CTGCCGGCGG 
GAACGAGAGC GCCGGCAGGA GCGGGGCCGG TCCCAGGAGC GGAGGCAGCC CTCATCCTCC 
TCCTCGGAGA AGCAGCGCTT CTACTCCTGC GACCGCTTTG GGGGCCGTGA GCCCCCGAAG 
CCCAAGCCCT CCCTCAGCAG CCACCCAACG TCGCCAACAG CTGGCCAGGA GCCGGGACCC 
CACCCACAGG GCAGTGGTTC CGTGAATGGG AGCCCCTTGC TGTCAACATC TGGTGCTAGC 
ACCCCCGGCC GCGGTGGGCG GAGGCAGCTC CCCCAGACGC CCCTGACTCC CCGCCCCAGC 
ATCACCTACA AGACGGCCAA CTCCTCACCC ATCCACTTCG CCGGGGCTCA GACCAGCCTC 
CCTGCCTTCT CCCCAGGCCG GCTCAGCCGT GGGCTTTCCG AACACAACGC CCTGCTGCAG 
AGAGACCCCC TCAGCCAGCC CCTGGCCCCT GGCTCTCGAA TTGGCTCTGA CCCTTACCTG 
GGGCAGCGTC TGGACAGTGA GGCCTCTGTC CACGCCCTGC CTGAGGACAC TCTCACTTTC 
GAGGAGGCTG TGGCCACCAA CTCGGGCCGC TCCTCCAGGA CTTCCTACGT GTCCTCCCTG 
ACCTCCCAGT CTCACCCTCT CCGCCGCGTG CCCAACGGTT ACCACTGCAC CCTGGGACTC 
AGCTCGGGTG GCCGAGCACG GCACAGCTAC CACCACCCTG ACCAAGACCA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( NM_000718.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000709.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant 1, mRNA.

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CloneID	OHu28838
Clone ID Related Accession (Same CDS sequence)	NM_000718.3 , NM_000718.4
Accession Version	NM_000718.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL772363.15, M94172.1, AL591424.12 and BC033060.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000718.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M94172.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000371372.6/ ENSP00000360423.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC AGGATCCAAA 
GGGAAAGACA TCAATACCAT CAAGTCTCTG AGAGTCCTTC GTGTCCTGCG GCCCCTCAAG 
ACCATCAAAC GGCTGCCCAA GCTCAAGGCT GTGTTTGACT GTGTGGTGAA CTCCCTGAAG 
AATGTCCTCA ACATCTTGAT TGTCTACATG CTCTTCATGT TCATATTTGC CGTCATTGCG 
GTGCAGCTCT TCAAAGGGAA GTTTTTCTAC TGCACAGATG AATCCAAGGA GCTGGAGAGG 
GACTGCAGGG GTCAGTATTT GGATTATGAG AAGGAGGAAG TGGAAGCTCA GCCCAGGCAG 
TGGAAGAAAT ACGACTTTCA CTACGACAAT GTGCTCTGGG CTCTGCTGAC GCTGTTCACA 
GTGTCCACGG GAGAAGGCTG GCCCATGGTG CTGAAACACT CCGTGGATGC CACCTATGAG 
GAGCAGGGTC CAAGCCCTGG GTACCGCATG GAGCTGTCCA TCTTCTACGT GGTCTACTTT 
GTGGTCTTTC CCTTCTTCTT CGTCAACATC TTTGTGGCTT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGGTGAT GTCTGAATGC AGCCTGGAGA AGAACGAGAG GGCTTGCATT 
GACTTCGCCA TCAGCGCCAA ACCCCTGACA CGGTACATGC CCCAAAACCG GCAGTCGTTC 
CAGTATAAGA CGTGGACATT TGTGGTCTCC CCGCCCTTTG AATACTTCAT CATGGCCATG 
ATAGCCCTCA ACACTGTGGT GCTGATGATG AAGTTCTATG ATGCACCCTA TGAGTACGAG 
CTGATGCTGA AATGCCTGAA CATCGTGTTC ACATCCATGT TCTCCATGGA ATGCGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAACTAT TTCAGAGATG CCTGGAATGT CTTTGACTTT 
GTCACTGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA AACGAACAAT 
TTCATCAACC TCAGCTTCCT CCGCCTCTTT CGAGCTGCGC GGCTGATCAA GCTGCTCCGC 
CAGGGCTACA CCATCCGCAT CCTGCTGTGG ACCTTTGTCC AGTCCTTCAA GGCCCTGCCC 
TACGTGTGTC TGCTCATTGC CATGCTGTTC TTCATCTACG CCATCATCGG CATGCAGGTG 
TTTGGGAATA TTGCCCTGGA TGATGACACC AGCATCAACC GCCACAACAA CTTCCGGACG 
TTTTTGCAAG CCCTGATGCT GCTGTTCAGG AGCGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCCT GCCTGAGCAA CCAGGCCTGT GATGAGCAGG CCAATGCCAC CGAGTGTGGA 
AGTGACTTTG CCTACTTCTA CTTCGTCTCC TTCATCTTCC TGTGCTCCTT TCTGATGTTG 
AACCTCTTTG TGGCTGTGAT CATGGACAAT TTTGAGTACC TCACGCGGGA CTCTTCCATC 
CTAGGTCCTC ACCACTTGGA TGAGTTCATC CGGGTCTGGG CTGAATACGA CCCGGCTGCG 
TGTGGGCGCA TCAGTTACAA TGACATGTTT GAGATGCTGA AACACATGTC CCCGCCTCTG 
GGGCTGGGGA AGAAATGCCC TGCTCGAGTT GCTTACAAGC GCCTGGTTCG CATGAACATG 
CCCATCTCCA ACGAGGACAT GACTGTTCAC TTCACGTCCA CGCTGATGGC CCTCATCCGG 
ACGGCACTGG AGATCAAGCT GGCCCCAGCT GGGACAAAGC AGCATCAGTG TGACGCGGAG 
TTGAGGAAGG AGATTTCCGT TGTGTGGGCC AATCTGCCCC AGAAGACTTT GGACTTGCTG 
GTACCACCCC ATAAGCCTGA TGAGATGACA GTGGGGAAGG TTTATGCAGC TCTGATGATA 
TTCGACTTCT ACAAGCAGAA CAAAACCACC AGAGACCAGA TGCAGCAGGC TCCTGGAGGC 
CTCTCCCAGA TGGGTCCTGT GTCCCTGTTC CACCCTCTGA AGGCCACCCT GGAGCAGACA 
CAGCCGGCTG TGCTCCGAGG AGCCCGGGTT TTCCTTCGAC AGAAGAGTTC CACCTCCCTC 
AGCAATGGCG GGGCCATACA AAACCAAGAG AGTGGCATCA AAGAGTCTGT CTCCTGGGGC 
ACTCAAAGGA CCCAGGATGC ACCCCATGAG GCCAGGCCAC CCCTGGAGCG TGGCCACTCC 
ACAGAGATCC CTGTGGGGCG GTCAGGAGCA CTGGCTGTGG ACGTTCAGAT GCAGAGCATA 
ACCCGGAGGG GCCCTGATGG GGAGCCCCAG CCTGGGCTGG AGAGCCAGGG TCGAGCGGCC 
TCCATGCCCC GCCTTGCGGC CGAGACTCAG CCCGTCACAG ATGCCAGCCC CATGAAGCGC 
TCCATCTCCA CGCTGGCCCA GCGGCCCCGT GGGACTCATC TTTGCAGCAC CACCCCGGAC 
CGCCCACCCC CTAGCCAGGC GTCGTCGCAC CACCACCACC ACCGCTGCCA CCGCCGCAGG 
GACAGGAAGC AGAGGTCCCT GGAGAAGGGG CCCAGCCTGT CTGCCGATAT GGATGGCGCA 
CCAAGCAGTG CTGTGGGGCC GGGGCTGCCC CCGGGAGAGG GGCCTACAGG CTGCCGGCGG 
GAACGAGAGC GCCGGCAGGA GCGGGGCCGG TCCCAGGAGC GGAGGCAGCC CTCATCCTCC 
TCCTCGGAGA AGCAGCGCTT CTACTCCTGC GACCGCTTTG GGGGCCGTGA GCCCCCGAAG 
CCCAAGCCCT CCCTCAGCAG CCACCCAACG TCGCCAACAG CTGGCCAGGA GCCGGGACCC 
CACCCACAGG GCAGTGGTTC CGTGAATGGG AGCCCCTTGC TGTCAACATC TGGTGCTAGC 
ACCCCCGGCC GCGGTGGGCG GAGGCAGCTC CCCCAGACGC CCCTGACTCC CCGCCCCAGC 
ATCACCTACA AGACGGCCAA CTCCTCACCC ATCCACTTCG CCGGGGCTCA GACCAGCCTC 
CCTGCCTTCT CCCCAGGCCG GCTCAGCCGT GGGCTTTCCG AACACAACGC CCTGCTGCAG 
AGAGACCCCC TCAGCCAGCC CCTGGCCCCT GGCTCTCGAA TTGGCTCTGA CCCTTACCTG 
GGGCAGCGTC TGGACAGTGA GGCCTCTGTC CACGCCCTGC CTGAGGACAC TCTCACTTTC 
GAGGAGGCTG TGGCCACCAA CTCGGGCCGC TCCTCCAGGA CTTCCTACGT GTCCTCCCTG 
ACCTCCCAGT CTCACCCTCT CCGCCGCGTG CCCAACGGTT ACCACTGCAC CCTGGGACTC 
AGCTCGGGTG GCCGAGCACG GCACAGCTAC CACCACCCTG ACCAAGACCA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000709.1
CDS	153..7172
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPIHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPGSRIGSDPYLGQRLDSEASVHALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	NM_000718.4
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000718.4

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC AGGATCCAAA 
GGGAAAGACA TCAATACCAT CAAGTCTCTG AGAGTCCTTC GTGTCCTGCG GCCCCTCAAG 
ACCATCAAAC GGCTGCCCAA GCTCAAGGCT GTGTTTGACT GTGTGGTGAA CTCCCTGAAG 
AATGTCCTCA ACATCTTGAT TGTCTACATG CTCTTCATGT TCATATTTGC CGTCATTGCG 
GTGCAGCTCT TCAAAGGGAA GTTTTTCTAC TGCACAGATG AATCCAAGGA GCTGGAGAGG 
GACTGCAGGG GTCAGTATTT GGATTATGAG AAGGAGGAAG TGGAAGCTCA GCCCAGGCAG 
TGGAAGAAAT ACGACTTTCA CTACGACAAT GTGCTCTGGG CTCTGCTGAC GCTGTTCACA 
GTGTCCACGG GAGAAGGCTG GCCCATGGTG CTGAAACACT CCGTGGATGC CACCTATGAG 
GAGCAGGGTC CAAGCCCTGG GTACCGCATG GAGCTGTCCA TCTTCTACGT GGTCTACTTT 
GTGGTCTTTC CCTTCTTCTT CGTCAACATC TTTGTGGCTT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGGTGAT GTCTGAATGC AGCCTGGAGA AGAACGAGAG GGCTTGCATT 
GACTTCGCCA TCAGCGCCAA ACCCCTGACA CGGTACATGC CCCAAAACCG GCAGTCGTTC 
CAGTATAAGA CGTGGACATT TGTGGTCTCC CCGCCCTTTG AATACTTCAT CATGGCCATG 
ATAGCCCTCA ACACTGTGGT GCTGATGATG AAGTTCTATG ATGCACCCTA TGAGTACGAG 
CTGATGCTGA AATGCCTGAA CATCGTGTTC ACATCCATGT TCTCCATGGA ATGCGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAACTAT TTCAGAGATG CCTGGAATGT CTTTGACTTT 
GTCACTGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA AACGAACAAT 
TTCATCAACC TCAGCTTCCT CCGCCTCTTT CGAGCTGCGC GGCTGATCAA GCTGCTCCGC 
CAGGGCTACA CCATCCGCAT CCTGCTGTGG ACCTTTGTCC AGTCCTTCAA GGCCCTGCCC 
TACGTGTGTC TGCTCATTGC CATGCTGTTC TTCATCTACG CCATCATCGG CATGCAGGTG 
TTTGGGAATA TTGCCCTGGA TGATGACACC AGCATCAACC GCCACAACAA CTTCCGGACG 
TTTTTGCAAG CCCTGATGCT GCTGTTCAGG AGCGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCCT GCCTGAGCAA CCAGGCCTGT GATGAGCAGG CCAATGCCAC CGAGTGTGGA 
AGTGACTTTG CCTACTTCTA CTTCGTCTCC TTCATCTTCC TGTGCTCCTT TCTGATGTTG 
AACCTCTTTG TGGCTGTGAT CATGGACAAT TTTGAGTACC TCACGCGGGA CTCTTCCATC 
CTAGGTCCTC ACCACTTGGA TGAGTTCATC CGGGTCTGGG CTGAATACGA CCCGGCTGCG 
TGTGGGCGCA TCAGTTACAA TGACATGTTT GAGATGCTGA AACACATGTC CCCGCCTCTG 
GGGCTGGGGA AGAAATGCCC TGCTCGAGTT GCTTACAAGC GCCTGGTTCG CATGAACATG 
CCCATCTCCA ACGAGGACAT GACTGTTCAC TTCACGTCCA CGCTGATGGC CCTCATCCGG 
ACGGCACTGG AGATCAAGCT GGCCCCAGCT GGGACAAAGC AGCATCAGTG TGACGCGGAG 
TTGAGGAAGG AGATTTCCGT TGTGTGGGCC AATCTGCCCC AGAAGACTTT GGACTTGCTG 
GTACCACCCC ATAAGCCTGA TGAGATGACA GTGGGGAAGG TTTATGCAGC TCTGATGATA 
TTCGACTTCT ACAAGCAGAA CAAAACCACC AGAGACCAGA TGCAGCAGGC TCCTGGAGGC 
CTCTCCCAGA TGGGTCCTGT GTCCCTGTTC CACCCTCTGA AGGCCACCCT GGAGCAGACA 
CAGCCGGCTG TGCTCCGAGG AGCCCGGGTT TTCCTTCGAC AGAAGAGTTC CACCTCCCTC 
AGCAATGGCG GGGCCATACA AAACCAAGAG AGTGGCATCA AAGAGTCTGT CTCCTGGGGC 
ACTCAAAGGA CCCAGGATGC ACCCCATGAG GCCAGGCCAC CCCTGGAGCG TGGCCACTCC 
ACAGAGATCC CTGTGGGGCG GTCAGGAGCA CTGGCTGTGG ACGTTCAGAT GCAGAGCATA 
ACCCGGAGGG GCCCTGATGG GGAGCCCCAG CCTGGGCTGG AGAGCCAGGG TCGAGCGGCC 
TCCATGCCCC GCCTTGCGGC CGAGACTCAG CCCGTCACAG ATGCCAGCCC CATGAAGCGC 
TCCATCTCCA CGCTGGCCCA GCGGCCCCGT GGGACTCATC TTTGCAGCAC CACCCCGGAC 
CGCCCACCCC CTAGCCAGGC GTCGTCGCAC CACCACCACC ACCGCTGCCA CCGCCGCAGG 
GACAGGAAGC AGAGGTCCCT GGAGAAGGGG CCCAGCCTGT CTGCCGATAT GGATGGCGCA 
CCAAGCAGTG CTGTGGGGCC GGGGCTGCCC CCGGGAGAGG GGCCTACAGG CTGCCGGCGG 
GAACGAGAGC GCCGGCAGGA GCGGGGCCGG TCCCAGGAGC GGAGGCAGCC CTCATCCTCC 
TCCTCGGAGA AGCAGCGCTT CTACTCCTGC GACCGCTTTG GGGGCCGTGA GCCCCCGAAG 
CCCAAGCCCT CCCTCAGCAG CCACCCAACG TCGCCAACAG CTGGCCAGGA GCCGGGACCC 
CACCCACAGG GCAGTGGTTC CGTGAATGGG AGCCCCTTGC TGTCAACATC TGGTGCTAGC 
ACCCCCGGCC GCGGTGGGCG GAGGCAGCTC CCCCAGACGC CCCTGACTCC CCGCCCCAGC 
ATCACCTACA AGACGGCCAA CTCCTCACCC ATCCACTTCG CCGGGGCTCA GACCAGCCTC 
CCTGCCTTCT CCCCAGGCCG GCTCAGCCGT GGGCTTTCCG AACACAACGC CCTGCTGCAG 
AGAGACCCCC TCAGCCAGCC CCTGGCCCCT GGCTCTCGAA TTGGCTCTGA CCCTTACCTG 
GGGCAGCGTC TGGACAGTGA GGCCTCTGTC CACGCCCTGC CTGAGGACAC TCTCACTTTC 
GAGGAGGCTG TGGCCACCAA CTCGGGCCGC TCCTCCAGGA CTTCCTACGT GTCCTCCCTG 
ACCTCCCAGT CTCACCCTCT CCGCCGCGTG CCCAACGGTT ACCACTGCAC CCTGGGACTC 
AGCTCGGGTG GCCGAGCACG GCACAGCTAC CACCACCCTG ACCAAGACCA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( NM_001243812.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230741.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant 2, mRNA.

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CloneID	OHu28817
Clone ID Related Accession (Same CDS sequence)	NM_001243812.2 , NM_001243812.1
Accession Version	NM_001243812.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL772363.15, M94173.1, AL591424.12 and BC033060.1. On May 31, 2019 this sequence version replaced NM_001243812.1. Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M94173.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC AGGATCCAAA 
GGGAAAGACA TCAATACCAT CAAGTCTCTG AGAGTCCTTC GTGTCCTGCG GCCCCTCAAG 
ACCATCAAAC GGCTGCCCAA GCTCAAGGCT GTGTTTGACT GTGTGGTGAA CTCCCTGAAG 
AATGTCCTCA ACATCTTGAT TGTCTACATG CTCTTCATGT TCATATTTGC CGTCATTGCG 
GTGCAGCTCT TCAAAGGGAA GTTTTTCTAC TGCACAGATG AATCCAAGGA GCTGGAGAGG 
GACTGCAGGG GTCAGTATTT GGATTATGAG AAGGAGGAAG TGGAAGCTCA GCCCAGGCAG 
TGGAAGAAAT ACGACTTTCA CTACGACAAT GTGCTCTGGG CTCTGCTGAC GCTGTTCACA 
GTGTCCACGG GAGAAGGCTG GCCCATGGTG CTGAAACACT CCGTGGATGC CACCTATGAG 
GAGCAGGGTC CAAGCCCTGG GTACCGCATG GAGCTGTCCA TCTTCTACGT GGTCTACTTT 
GTGGTCTTTC CCTTCTTCTT CGTCAACATC TTTGTGGCTT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGGTGAT GTCTGAATGC AGCCTGGAGA AGAACGAGAG GGCTTGCATT 
GACTTCGCCA TCAGCGCCAA ACCCCTGACA CGGTACATGC CCCAAAACCG GCAGTCGTTC 
CAGTATAAGA CGTGGACATT TGTGGTCTCC CCGCCCTTTG AATACTTCAT CATGGCCATG 
ATAGCCCTCA ACACTGTGGT GCTGATGATG AAGTTCTATG ATGCACCCTA TGAGTACGAG 
CTGATGCTGA AATGCCTGAA CATCGTGTTC ACATCCATGT TCTCCATGGA ATGCGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAACTAT TTCAGAGATG CCTGGAATGT CTTTGACTTT 
GTCACTGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA AACGAACAAT 
TTCATCAACC TCAGCTTCCT CCGCCTCTTT CGAGCTGCGC GGCTGATCAA GCTGCTCCGC 
CAGGGCTACA CCATCCGCAT CCTGCTGTGG ACCTTTGTCC AGTCCTTCAA GGCCCTGCCC 
TACGTGTGTC TGCTCATTGC CATGCTGTTC TTCATCTACG CCATCATCGG CATGCAGGTG 
TTTGGGAATA TTGCCCTGGA TGATGACACC AGCATCAACC GCCACAACAA CTTCCGGACG 
TTTTTGCAAG CCCTGATGCT GCTGTTCAGG AGCGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCCT GCCTGAGCAA CCAGGCCTGT GATGAGCAGG CCAATGCCAC CGAGTGTGGA 
AGTGACTTTG CCTACTTCTA CTTCGTCTCC TTCATCTTCC TGTGCTCCTT TCTGATGTTG 
AACCTCTTTG TGGCTGTGAT CATGGACAAT TTTGAGTACC TCACGCGGGA CTCTTCCATC 
CTAGGTCCTC ACCACTTGGA TGAGTTCATC CGGGTCTGGG CTGAATACGA CCCGGCTGCG 
TGTGGGCGCA TCAGTTACAA TGACATGTTT GAGATGCTGA AACACATGTC CCCGCCTCTG 
GGGCTGGGGA AGAAATGCCC TGCTCGAGTT GCTTACAAGC GCCTGGTTCG CATGAACATG 
CCCATCTCCA ACGAGGACAT GACTGTTCAC TTCACGTCCA CGCTGATGGC CCTCATCCGG 
ACGGCACTGG AGATCAAGCT GGCCCCAGCT GGGACAAAGC AGCATCAGTG TGACGCGGAG 
TTGAGGAAGG AGATTTCCGT TGTGTGGGCC AATCTGCCCC AGAAGACTTT GGACTTGCTG 
GTACCACCCC ATAAGCCTGA TGAGATGACA GTGGGGAAGG TTTATGCAGC TCTGATGATA 
TTCGACTTCT ACAAGCAGAA CAAAACCACC AGAGACCAGA TGCAGCAGGC TCCTGGAGGC 
CTCTCCCAGA TGGGTCCTGT GTCCCTGTTC CACCCTCTGA AGGCCACCCT GGAGCAGACA 
CAGCCGGCTG TGCTCCGAGG AGCCCGGGTT TTCCTTCGAC AGAAGAGTTC CACCTCCCTC 
AGCAATGGCG GGGCCATACA AAACCAAGAG AGTGGCATCA AAGAGTCTGT CTCCTGGGGC 
ACTCAAAGGA CCCAGGATGC ACCCCATGAG GCCAGGCCAC CCCTGGAGCG TGGCCACTCC 
ACAGAGATCC CTGTGGGGCG GTCAGGAGCA CTGGCTGTGG ACGTTCAGAT GCAGAGCATA 
ACCCGGAGGG GCCCTGATGG GGAGCCCCAG CCTGGGCTGG AGAGCCAGGG TCGAGCGGCC 
TCCATGCCCC GCCTTGCGGC CGAGACTCAG CCCGTCACAG ATGCCAGCCC CATGAAGCGC 
TCCATCTCCA CGCTGGCCCA GCGGCCCCGT GGGACTCATC TTTGCAGCAC CACCCCGGAC 
CGCCCACCCC CTAGCCAGGC GTCGTCGCAC CACCACCACC ACCGCTGCCA CCGCCGCAGG 
GACAGGAAGC AGAGGTCCCT GGAGAAGGGG CCCAGCCTGT CTGCCGATAT GGATGGCGCA 
CCAAGCAGTG CTGTGGGGCC GGGGCTGCCC CCGGGAGAGG GGCCTACAGG CTGCCGGCGG 
GAACGAGAGC GCCGGCAGGA GCGGGGCCGG TCCCAGGAGC GGAGGCAGCC CTCATCCTCC 
TCCTCGGAGA AGCAGCGCTT CTACTCCTGC GACCGCTTTG GGGGCCGTGA GCCCCCGAAG 
CCCAAGCCCT CCCTCAGCAG CCACCCAACG TCGCCAACAG CTGGCCAGGA GCCGGGACCC 
CACCCACAGG CCGGCTCAGC CGTGGGCTTT CCGAACACAA CGCCCTGCTG CAGAGAGACC 
CCCTCAGCCA GCCCCTGGCC CCTGGCTCTC GAATTGGCTC TGACCCTTAC CTGGGGCAGC 
GTCTGGACAG TGAGGCCTCT GTCCACGCCC TGCCTGAGGA CACTCTCACT TTCGAGGAGG 
CTGTGGCCAC CAACTCGGGC CGCTCCTCCA GGACTTCCTA CGTGTCCTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230741.1
CDS	153..6866
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQAGSAVGFPNTTPCCRETPSASPWPLALELALTLTWGSVWTVRPLSTPCLRTLSLSRRLWPPTRAAPPGLPTCPP

Target ORF information:

RefSeq Version	NM_001243812.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243812.2

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC AGGATCCAAA 
GGGAAAGACA TCAATACCAT CAAGTCTCTG AGAGTCCTTC GTGTCCTGCG GCCCCTCAAG 
ACCATCAAAC GGCTGCCCAA GCTCAAGGCT GTGTTTGACT GTGTGGTGAA CTCCCTGAAG 
AATGTCCTCA ACATCTTGAT TGTCTACATG CTCTTCATGT TCATATTTGC CGTCATTGCG 
GTGCAGCTCT TCAAAGGGAA GTTTTTCTAC TGCACAGATG AATCCAAGGA GCTGGAGAGG 
GACTGCAGGG GTCAGTATTT GGATTATGAG AAGGAGGAAG TGGAAGCTCA GCCCAGGCAG 
TGGAAGAAAT ACGACTTTCA CTACGACAAT GTGCTCTGGG CTCTGCTGAC GCTGTTCACA 
GTGTCCACGG GAGAAGGCTG GCCCATGGTG CTGAAACACT CCGTGGATGC CACCTATGAG 
GAGCAGGGTC CAAGCCCTGG GTACCGCATG GAGCTGTCCA TCTTCTACGT GGTCTACTTT 
GTGGTCTTTC CCTTCTTCTT CGTCAACATC TTTGTGGCTT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGGTGAT GTCTGAATGC AGCCTGGAGA AGAACGAGAG GGCTTGCATT 
GACTTCGCCA TCAGCGCCAA ACCCCTGACA CGGTACATGC CCCAAAACCG GCAGTCGTTC 
CAGTATAAGA CGTGGACATT TGTGGTCTCC CCGCCCTTTG AATACTTCAT CATGGCCATG 
ATAGCCCTCA ACACTGTGGT GCTGATGATG AAGTTCTATG ATGCACCCTA TGAGTACGAG 
CTGATGCTGA AATGCCTGAA CATCGTGTTC ACATCCATGT TCTCCATGGA ATGCGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAACTAT TTCAGAGATG CCTGGAATGT CTTTGACTTT 
GTCACTGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA AACGAACAAT 
TTCATCAACC TCAGCTTCCT CCGCCTCTTT CGAGCTGCGC GGCTGATCAA GCTGCTCCGC 
CAGGGCTACA CCATCCGCAT CCTGCTGTGG ACCTTTGTCC AGTCCTTCAA GGCCCTGCCC 
TACGTGTGTC TGCTCATTGC CATGCTGTTC TTCATCTACG CCATCATCGG CATGCAGGTG 
TTTGGGAATA TTGCCCTGGA TGATGACACC AGCATCAACC GCCACAACAA CTTCCGGACG 
TTTTTGCAAG CCCTGATGCT GCTGTTCAGG AGCGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCCT GCCTGAGCAA CCAGGCCTGT GATGAGCAGG CCAATGCCAC CGAGTGTGGA 
AGTGACTTTG CCTACTTCTA CTTCGTCTCC TTCATCTTCC TGTGCTCCTT TCTGATGTTG 
AACCTCTTTG TGGCTGTGAT CATGGACAAT TTTGAGTACC TCACGCGGGA CTCTTCCATC 
CTAGGTCCTC ACCACTTGGA TGAGTTCATC CGGGTCTGGG CTGAATACGA CCCGGCTGCG 
TGTGGGCGCA TCAGTTACAA TGACATGTTT GAGATGCTGA AACACATGTC CCCGCCTCTG 
GGGCTGGGGA AGAAATGCCC TGCTCGAGTT GCTTACAAGC GCCTGGTTCG CATGAACATG 
CCCATCTCCA ACGAGGACAT GACTGTTCAC TTCACGTCCA CGCTGATGGC CCTCATCCGG 
ACGGCACTGG AGATCAAGCT GGCCCCAGCT GGGACAAAGC AGCATCAGTG TGACGCGGAG 
TTGAGGAAGG AGATTTCCGT TGTGTGGGCC AATCTGCCCC AGAAGACTTT GGACTTGCTG 
GTACCACCCC ATAAGCCTGA TGAGATGACA GTGGGGAAGG TTTATGCAGC TCTGATGATA 
TTCGACTTCT ACAAGCAGAA CAAAACCACC AGAGACCAGA TGCAGCAGGC TCCTGGAGGC 
CTCTCCCAGA TGGGTCCTGT GTCCCTGTTC CACCCTCTGA AGGCCACCCT GGAGCAGACA 
CAGCCGGCTG TGCTCCGAGG AGCCCGGGTT TTCCTTCGAC AGAAGAGTTC CACCTCCCTC 
AGCAATGGCG GGGCCATACA AAACCAAGAG AGTGGCATCA AAGAGTCTGT CTCCTGGGGC 
ACTCAAAGGA CCCAGGATGC ACCCCATGAG GCCAGGCCAC CCCTGGAGCG TGGCCACTCC 
ACAGAGATCC CTGTGGGGCG GTCAGGAGCA CTGGCTGTGG ACGTTCAGAT GCAGAGCATA 
ACCCGGAGGG GCCCTGATGG GGAGCCCCAG CCTGGGCTGG AGAGCCAGGG TCGAGCGGCC 
TCCATGCCCC GCCTTGCGGC CGAGACTCAG CCCGTCACAG ATGCCAGCCC CATGAAGCGC 
TCCATCTCCA CGCTGGCCCA GCGGCCCCGT GGGACTCATC TTTGCAGCAC CACCCCGGAC 
CGCCCACCCC CTAGCCAGGC GTCGTCGCAC CACCACCACC ACCGCTGCCA CCGCCGCAGG 
GACAGGAAGC AGAGGTCCCT GGAGAAGGGG CCCAGCCTGT CTGCCGATAT GGATGGCGCA 
CCAAGCAGTG CTGTGGGGCC GGGGCTGCCC CCGGGAGAGG GGCCTACAGG CTGCCGGCGG 
GAACGAGAGC GCCGGCAGGA GCGGGGCCGG TCCCAGGAGC GGAGGCAGCC CTCATCCTCC 
TCCTCGGAGA AGCAGCGCTT CTACTCCTGC GACCGCTTTG GGGGCCGTGA GCCCCCGAAG 
CCCAAGCCCT CCCTCAGCAG CCACCCAACG TCGCCAACAG CTGGCCAGGA GCCGGGACCC 
CACCCACAGG CCGGCTCAGC CGTGGGCTTT CCGAACACAA CGCCCTGCTG CAGAGAGACC 
CCCTCAGCCA GCCCCTGGCC CCTGGCTCTC GAATTGGCTC TGACCCTTAC CTGGGGCAGC 
GTCTGGACAG TGAGGCCTCT GTCCACGCCC TGCCTGAGGA CACTCTCACT TTCGAGGAGG 
CTGTGGCCAC CAACTCGGGC CGCTCCTCCA GGACTTCCTA CGTGTCCTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( NM_001243812.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230741.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant 2, mRNA.

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CloneID	OHu28817
Clone ID Related Accession (Same CDS sequence)	NM_001243812.2 , NM_001243812.1
Accession Version	NM_001243812.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M94173.1, AL772363.15, AL591424.12 and BC033060.1. Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M94173.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC AGGATCCAAA 
GGGAAAGACA TCAATACCAT CAAGTCTCTG AGAGTCCTTC GTGTCCTGCG GCCCCTCAAG 
ACCATCAAAC GGCTGCCCAA GCTCAAGGCT GTGTTTGACT GTGTGGTGAA CTCCCTGAAG 
AATGTCCTCA ACATCTTGAT TGTCTACATG CTCTTCATGT TCATATTTGC CGTCATTGCG 
GTGCAGCTCT TCAAAGGGAA GTTTTTCTAC TGCACAGATG AATCCAAGGA GCTGGAGAGG 
GACTGCAGGG GTCAGTATTT GGATTATGAG AAGGAGGAAG TGGAAGCTCA GCCCAGGCAG 
TGGAAGAAAT ACGACTTTCA CTACGACAAT GTGCTCTGGG CTCTGCTGAC GCTGTTCACA 
GTGTCCACGG GAGAAGGCTG GCCCATGGTG CTGAAACACT CCGTGGATGC CACCTATGAG 
GAGCAGGGTC CAAGCCCTGG GTACCGCATG GAGCTGTCCA TCTTCTACGT GGTCTACTTT 
GTGGTCTTTC CCTTCTTCTT CGTCAACATC TTTGTGGCTT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGGTGAT GTCTGAATGC AGCCTGGAGA AGAACGAGAG GGCTTGCATT 
GACTTCGCCA TCAGCGCCAA ACCCCTGACA CGGTACATGC CCCAAAACCG GCAGTCGTTC 
CAGTATAAGA CGTGGACATT TGTGGTCTCC CCGCCCTTTG AATACTTCAT CATGGCCATG 
ATAGCCCTCA ACACTGTGGT GCTGATGATG AAGTTCTATG ATGCACCCTA TGAGTACGAG 
CTGATGCTGA AATGCCTGAA CATCGTGTTC ACATCCATGT TCTCCATGGA ATGCGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAACTAT TTCAGAGATG CCTGGAATGT CTTTGACTTT 
GTCACTGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA AACGAACAAT 
TTCATCAACC TCAGCTTCCT CCGCCTCTTT CGAGCTGCGC GGCTGATCAA GCTGCTCCGC 
CAGGGCTACA CCATCCGCAT CCTGCTGTGG ACCTTTGTCC AGTCCTTCAA GGCCCTGCCC 
TACGTGTGTC TGCTCATTGC CATGCTGTTC TTCATCTACG CCATCATCGG CATGCAGGTG 
TTTGGGAATA TTGCCCTGGA TGATGACACC AGCATCAACC GCCACAACAA CTTCCGGACG 
TTTTTGCAAG CCCTGATGCT GCTGTTCAGG AGCGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCCT GCCTGAGCAA CCAGGCCTGT GATGAGCAGG CCAATGCCAC CGAGTGTGGA 
AGTGACTTTG CCTACTTCTA CTTCGTCTCC TTCATCTTCC TGTGCTCCTT TCTGATGTTG 
AACCTCTTTG TGGCTGTGAT CATGGACAAT TTTGAGTACC TCACGCGGGA CTCTTCCATC 
CTAGGTCCTC ACCACTTGGA TGAGTTCATC CGGGTCTGGG CTGAATACGA CCCGGCTGCG 
TGTGGGCGCA TCAGTTACAA TGACATGTTT GAGATGCTGA AACACATGTC CCCGCCTCTG 
GGGCTGGGGA AGAAATGCCC TGCTCGAGTT GCTTACAAGC GCCTGGTTCG CATGAACATG 
CCCATCTCCA ACGAGGACAT GACTGTTCAC TTCACGTCCA CGCTGATGGC CCTCATCCGG 
ACGGCACTGG AGATCAAGCT GGCCCCAGCT GGGACAAAGC AGCATCAGTG TGACGCGGAG 
TTGAGGAAGG AGATTTCCGT TGTGTGGGCC AATCTGCCCC AGAAGACTTT GGACTTGCTG 
GTACCACCCC ATAAGCCTGA TGAGATGACA GTGGGGAAGG TTTATGCAGC TCTGATGATA 
TTCGACTTCT ACAAGCAGAA CAAAACCACC AGAGACCAGA TGCAGCAGGC TCCTGGAGGC 
CTCTCCCAGA TGGGTCCTGT GTCCCTGTTC CACCCTCTGA AGGCCACCCT GGAGCAGACA 
CAGCCGGCTG TGCTCCGAGG AGCCCGGGTT TTCCTTCGAC AGAAGAGTTC CACCTCCCTC 
AGCAATGGCG GGGCCATACA AAACCAAGAG AGTGGCATCA AAGAGTCTGT CTCCTGGGGC 
ACTCAAAGGA CCCAGGATGC ACCCCATGAG GCCAGGCCAC CCCTGGAGCG TGGCCACTCC 
ACAGAGATCC CTGTGGGGCG GTCAGGAGCA CTGGCTGTGG ACGTTCAGAT GCAGAGCATA 
ACCCGGAGGG GCCCTGATGG GGAGCCCCAG CCTGGGCTGG AGAGCCAGGG TCGAGCGGCC 
TCCATGCCCC GCCTTGCGGC CGAGACTCAG CCCGTCACAG ATGCCAGCCC CATGAAGCGC 
TCCATCTCCA CGCTGGCCCA GCGGCCCCGT GGGACTCATC TTTGCAGCAC CACCCCGGAC 
CGCCCACCCC CTAGCCAGGC GTCGTCGCAC CACCACCACC ACCGCTGCCA CCGCCGCAGG 
GACAGGAAGC AGAGGTCCCT GGAGAAGGGG CCCAGCCTGT CTGCCGATAT GGATGGCGCA 
CCAAGCAGTG CTGTGGGGCC GGGGCTGCCC CCGGGAGAGG GGCCTACAGG CTGCCGGCGG 
GAACGAGAGC GCCGGCAGGA GCGGGGCCGG TCCCAGGAGC GGAGGCAGCC CTCATCCTCC 
TCCTCGGAGA AGCAGCGCTT CTACTCCTGC GACCGCTTTG GGGGCCGTGA GCCCCCGAAG 
CCCAAGCCCT CCCTCAGCAG CCACCCAACG TCGCCAACAG CTGGCCAGGA GCCGGGACCC 
CACCCACAGG CCGGCTCAGC CGTGGGCTTT CCGAACACAA CGCCCTGCTG CAGAGAGACC 
CCCTCAGCCA GCCCCTGGCC CCTGGCTCTC GAATTGGCTC TGACCCTTAC CTGGGGCAGC 
GTCTGGACAG TGAGGCCTCT GTCCACGCCC TGCCTGAGGA CACTCTCACT TTCGAGGAGG 
CTGTGGCCAC CAACTCGGGC CGCTCCTCCA GGACTTCCTA CGTGTCCTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230741.1
CDS	146..6859
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQAGSAVGFPNTTPCCRETPSASPWPLALELALTLTWGSVWTVRPLSTPCLRTLSLSRRLWPPTRAAPPGLPTCPP

Target ORF information:

RefSeq Version	NM_001243812.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243812.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC AGGATCCAAA 
GGGAAAGACA TCAATACCAT CAAGTCTCTG AGAGTCCTTC GTGTCCTGCG GCCCCTCAAG 
ACCATCAAAC GGCTGCCCAA GCTCAAGGCT GTGTTTGACT GTGTGGTGAA CTCCCTGAAG 
AATGTCCTCA ACATCTTGAT TGTCTACATG CTCTTCATGT TCATATTTGC CGTCATTGCG 
GTGCAGCTCT TCAAAGGGAA GTTTTTCTAC TGCACAGATG AATCCAAGGA GCTGGAGAGG 
GACTGCAGGG GTCAGTATTT GGATTATGAG AAGGAGGAAG TGGAAGCTCA GCCCAGGCAG 
TGGAAGAAAT ACGACTTTCA CTACGACAAT GTGCTCTGGG CTCTGCTGAC GCTGTTCACA 
GTGTCCACGG GAGAAGGCTG GCCCATGGTG CTGAAACACT CCGTGGATGC CACCTATGAG 
GAGCAGGGTC CAAGCCCTGG GTACCGCATG GAGCTGTCCA TCTTCTACGT GGTCTACTTT 
GTGGTCTTTC CCTTCTTCTT CGTCAACATC TTTGTGGCTT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGGTGAT GTCTGAATGC AGCCTGGAGA AGAACGAGAG GGCTTGCATT 
GACTTCGCCA TCAGCGCCAA ACCCCTGACA CGGTACATGC CCCAAAACCG GCAGTCGTTC 
CAGTATAAGA CGTGGACATT TGTGGTCTCC CCGCCCTTTG AATACTTCAT CATGGCCATG 
ATAGCCCTCA ACACTGTGGT GCTGATGATG AAGTTCTATG ATGCACCCTA TGAGTACGAG 
CTGATGCTGA AATGCCTGAA CATCGTGTTC ACATCCATGT TCTCCATGGA ATGCGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAACTAT TTCAGAGATG CCTGGAATGT CTTTGACTTT 
GTCACTGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGGA AACGAACAAT 
TTCATCAACC TCAGCTTCCT CCGCCTCTTT CGAGCTGCGC GGCTGATCAA GCTGCTCCGC 
CAGGGCTACA CCATCCGCAT CCTGCTGTGG ACCTTTGTCC AGTCCTTCAA GGCCCTGCCC 
TACGTGTGTC TGCTCATTGC CATGCTGTTC TTCATCTACG CCATCATCGG CATGCAGGTG 
TTTGGGAATA TTGCCCTGGA TGATGACACC AGCATCAACC GCCACAACAA CTTCCGGACG 
TTTTTGCAAG CCCTGATGCT GCTGTTCAGG AGCGCCACGG GGGAGGCCTG GCACGAGATC 
ATGCTGTCCT GCCTGAGCAA CCAGGCCTGT GATGAGCAGG CCAATGCCAC CGAGTGTGGA 
AGTGACTTTG CCTACTTCTA CTTCGTCTCC TTCATCTTCC TGTGCTCCTT TCTGATGTTG 
AACCTCTTTG TGGCTGTGAT CATGGACAAT TTTGAGTACC TCACGCGGGA CTCTTCCATC 
CTAGGTCCTC ACCACTTGGA TGAGTTCATC CGGGTCTGGG CTGAATACGA CCCGGCTGCG 
TGTGGGCGCA TCAGTTACAA TGACATGTTT GAGATGCTGA AACACATGTC CCCGCCTCTG 
GGGCTGGGGA AGAAATGCCC TGCTCGAGTT GCTTACAAGC GCCTGGTTCG CATGAACATG 
CCCATCTCCA ACGAGGACAT GACTGTTCAC TTCACGTCCA CGCTGATGGC CCTCATCCGG 
ACGGCACTGG AGATCAAGCT GGCCCCAGCT GGGACAAAGC AGCATCAGTG TGACGCGGAG 
TTGAGGAAGG AGATTTCCGT TGTGTGGGCC AATCTGCCCC AGAAGACTTT GGACTTGCTG 
GTACCACCCC ATAAGCCTGA TGAGATGACA GTGGGGAAGG TTTATGCAGC TCTGATGATA 
TTCGACTTCT ACAAGCAGAA CAAAACCACC AGAGACCAGA TGCAGCAGGC TCCTGGAGGC 
CTCTCCCAGA TGGGTCCTGT GTCCCTGTTC CACCCTCTGA AGGCCACCCT GGAGCAGACA 
CAGCCGGCTG TGCTCCGAGG AGCCCGGGTT TTCCTTCGAC AGAAGAGTTC CACCTCCCTC 
AGCAATGGCG GGGCCATACA AAACCAAGAG AGTGGCATCA AAGAGTCTGT CTCCTGGGGC 
ACTCAAAGGA CCCAGGATGC ACCCCATGAG GCCAGGCCAC CCCTGGAGCG TGGCCACTCC 
ACAGAGATCC CTGTGGGGCG GTCAGGAGCA CTGGCTGTGG ACGTTCAGAT GCAGAGCATA 
ACCCGGAGGG GCCCTGATGG GGAGCCCCAG CCTGGGCTGG AGAGCCAGGG TCGAGCGGCC 
TCCATGCCCC GCCTTGCGGC CGAGACTCAG CCCGTCACAG ATGCCAGCCC CATGAAGCGC 
TCCATCTCCA CGCTGGCCCA GCGGCCCCGT GGGACTCATC TTTGCAGCAC CACCCCGGAC 
CGCCCACCCC CTAGCCAGGC GTCGTCGCAC CACCACCACC ACCGCTGCCA CCGCCGCAGG 
GACAGGAAGC AGAGGTCCCT GGAGAAGGGG CCCAGCCTGT CTGCCGATAT GGATGGCGCA 
CCAAGCAGTG CTGTGGGGCC GGGGCTGCCC CCGGGAGAGG GGCCTACAGG CTGCCGGCGG 
GAACGAGAGC GCCGGCAGGA GCGGGGCCGG TCCCAGGAGC GGAGGCAGCC CTCATCCTCC 
TCCTCGGAGA AGCAGCGCTT CTACTCCTGC GACCGCTTTG GGGGCCGTGA GCCCCCGAAG 
CCCAAGCCCT CCCTCAGCAG CCACCCAACG TCGCCAACAG CTGGCCAGGA GCCGGGACCC 
CACCCACAGG CCGGCTCAGC CGTGGGCTTT CCGAACACAA CGCCCTGCTG CAGAGAGACC 
CCCTCAGCCA GCCCCTGGCC CCTGGCTCTC GAATTGGCTC TGACCCTTAC CTGGGGCAGC 
GTCTGGACAG TGAGGCCTCT GTCCACGCCC TGCCTGAGGA CACTCTCACT TTCGAGGAGG 
CTGTGGCCAC CAACTCGGGC CGCTCCTCCA GGACTTCCTA CGTGTCCTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_011518991.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517293.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X1, mRNA.

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CloneID	OHu56562
Clone ID Related Accession (Same CDS sequence)	XM_011518991.2
Accession Version	XM_011518991.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGCAGTGCT GAAGAGAGCG 
GCCACCAAGA AGAGCAGAAA TGACCTGATC CACGCAGAGG AGGGAGAGGA CCGGTTTGCA 
GATCTCTGTG CTGTTGGATC CCCCTTCGCC CGCGCCAGCC TCAAGAGCGG GAAGACAGAG 
AGCTCGTCAT ACTTCCGGAG GAAGGAGAAG ATGTTCCGGT TTTTTATCCG GCGCATGGTG 
AAGGCTCAGA GCTTCTACTG GGTGGTGCTG TGCGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ATTACAACCA GCCGCGGCGG CTTACCACGA CCCTGTATTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGA 
AGCTACTTCC GGTCCTCCTT CAACTGCTTC GACTTTGGGG TCATCGTGGG GAGCGTCTTT 
GAAGTGGTCT GGGCGGCCAT CAAGCCGGGA AGCTCCTTTG GGATCAGTGT GCTGCGGGCC 
CTCCGCCTGC TGAGGATCTT CAAAGTCACG AAGTACTGGA GCTCCCTGCG GAACCTGGTG 
GTGTCCCTGC TGAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCTTGCT CTTCCTGTTC 
ATTGTGGTCT TCGCCCTGCT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTCCAGGAT 
GAGACTCCCA CAACCAACTT CGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC 
CTGACGGGAG AGGACTGGAA TGCAGTGATG TATCACGGGA TCGAATCGCA AGGCGGCGTC 
AGCAAAGGCA TGTTCTCGTC CTTTTACTTC ATTGTCCTGA CACTGTTCGG AAACTACACT 
CTGCTGAATG TCTTTCTGGC CATCGCTGTG GACAACCTGG CCAACGCCCA AGAGCTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA 
GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATCGCCGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTACG GCTGCAGAAC 
CTGCGGGCCA GCTGCGAGGC GCTGTACAGC GAGATGGACC CCGAGGAGCG GCTGCGCTTC 
GCCACTACGC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTGGTGGTG 
GAGCTGGGCC GCGACGGCGC GCGGGGGCCC GTGGGAGGCA AAGCCCGACC TGAGGCTGCG 
GAGGCCCCCG AGGGCGTCGA CCCTCCGCGC AGGCACCACC GGCACCGCGA CAAGGACAAG 
ACCCCCGCGG CGGGGGACCA GGACCGAGCA GAGGCCCCGA AGGCGGAGAG CGGGGAGCCC 
GGTGCCCGGG AGGAGCGGCC GCGGCCGCAC CGCAGCCACA GCAAGGAGGC CGCGGGGCCC 
CCGGAGGCGC GGAGCGAGCG CGGCCGAGGC CCAGGCCCCG AGGGCGGCCG GCGGCACCAC 
CGGCGCGGCT CCCCGGAGGA GGCGGCCGAG CGGGAGCCCC GACGCCACCG CGCGCACCGG 
CACCAGGATC CGAGCAAGGA GTGCGCCGGC GCCAAGGGCG AGCGGCGCGC GCGGCACCGC 
GGCGGCCCCC GAGCGGGGCC CCGGGAGGCG GAGAGCGGGG AGGAGCCGGC GCGGCGGCAC 
CGGGCCCGGC ACAAGGCGCA GCCTGCTCAC GAGGCTGTGG AGAAGGAGAC CACGGAGAAG 
GAGGCCACGG AGAAGGAGGC TGAGATAGTG GAAGCCGACA AGGAAAAGGA GCTCCGGAAC 
CACCAGCCCC GGGAGCCACA CTGTGACCTG GAGACCAGTG GGACTGTGAC TGTGGGTCCC 
ATGCACACAC TGCCCAGCAC CTGTCTCCAG AAGGTGGAGG AACAGCCAGA GGATGCAGAC 
AATCAGCGGA ACGTCACTCG CATGGGCAGT CAGCCCCCAG ACCCGAACAC TATTGTACAT 
ATCCCAGTGA TGCTGACGGG CCCTCTTGGG GAAGCCACGG TCGTTCCCAG TGGTAACGTG 
GACCTGGAAA GCCAAGCAGA GGGGAAGAAG GAGGTGGAAG CGGATGACGT GATGAGGAGC 
GGCCCCCGGC CTATCGTCCC ATACAGCTCC ATGTTCTGTT TAAGCCCCAC CAACCTGCTC 
CGCCGCTTCT GCCACTACAT CGTGACCATG AGGTACTTCG AGGTGGTCAT TCTCGTGGTC 
ATCGCCTTGA GCAGCATCGC CCTGGCTGCT GAGGACCCAG TGCGCACAGA CTCGCCCAGG 
AACAACGCTC TGAAATACCT GGATTACATT TTCACTGGTG TCTTTACCTT TGAGATGGTG 
ATAAAGATGA TCGACTTGGG ACTGCTGCTT CACCCTGGAG CCTATTTCCG GGACTTGTGG 
AACATTCTGG ACTTCATTGT GGTCAGTGGC GCCCTGGTGG CGTTTGCTTT CTCGAGCTTC 
GTGGGAGGAT CCAAAGGGAA AGACATCAAT ACCATCAAGT CTCTGAGAGT CCTTCGTGTC 
CTGCGGCCCC TCAAGACCAT CAAACGGCTG CCCAAGCTCA AGGCTGTGTT TGACTGTGTG 
GTGAACTCCC TGAAGAATGT CCTCAACATC TTGATTGTCT ACATGCTCTT CATGTTCATA 
TTTGCCGTCA TTGCGGTGCA GCTCTTCAAA GGGAAGTTTT TCTACTGCAC AGATGAATCC 
AAGGAGCTGG AGAGGGACTG CAGGGGTCAG TATTTGGATT ATGAGAAGGA GGAAGTGGAA 
GCTCAGCCCA GGCAGTGGAA GAAATACGAC TTTCACTACG ACAATGTGCT CTGGGCTCTG 
CTGACGCTGT TCACAGTGTC CACGGGAGAA GGCTGGCCCA TGGTGCTGAA ACACTCCGTG 
GATGCCACCT ATGAGGAGCA GGGTCCAAGC CCTGGGTACC GCATGGAGCT GTCCATCTTC 
TACGTGGTCT ACTTTGTGGT CTTTCCCTTC TTCTTCGTCA ACATCTTTGT GGCTTTGATC 
ATCATCACCT TCCAGGAGCA GGGGGACAAG GTGATGTCTG AATGCAGCCT GGAGAAGAAC 
GAGAGGGCTT GCATTGACTT CGCCATCAGC GCCAAACCCC TGACACGGTA CATGCCCCAA 
AACCGGCAGT CGTTCCAGTA TAAGACGTGG ACATTTGTGG TCTCCCCGCC CTTTGAATAC 
TTCATCATGG CCATGATAGC CCTCAACACT GTGGTGCTGA TGATGAAGTT CTATGATGCA 
CCCTATGAGT ACGAGCTGAT GCTGAAATGC CTGAACATCG TGTTCACATC CATGTTCTCC 
ATGGAATGCG TGCTGAAGAT CATCGCCTTT GGGGTGCTGA ACTATTTCAG AGATGCCTGG 
AATGTCTTTG ACTTTGTCAC TGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGAAACGA ACAATTTCAT CAACCTCAGC TTCCTCCGCC TCTTTCGAGC TGCGCGGCTG 
ATCAAGCTGC TCCGCCAGGG CTACACCATC CGCATCCTGC TGTGGACCTT TGTCCAGTCC 
TTCAAGGCCC TGCCCTACGT GTGTCTGCTC ATTGCCATGC TGTTCTTCAT CTACGCCATC 
ATCGGCATGC AGGTGTTTGG GAATATTGCC CTGGATGATG ACACCAGCAT CAACCGCCAC 
AACAACTTCC GGACGTTTTT GCAAGCCCTG ATGCTGCTGT TCAGGAGCGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCCTGCCTG AGCAACCAGG CCTGTGATGA GCAGGCCAAT 
GCCACCGAGT GTGGAAGTGA CTTTGCCTAC TTCTACTTCG TCTCCTTCAT CTTCCTGTGC 
TCCTTTCTGA TGTTGAACCT CTTTGTGGCT GTGATCATGG ACAATTTTGA GTACCTCACG 
CGGGACTCTT CCATCCTAGG TCCTCACCAC TTGGATGAGT TCATCCGGGT CTGGGCTGAA 
TACGACCCGG CTGCGTGTGG GCGCATCAGT TACAATGACA TGTTTGAGAT GCTGAAACAC 
ATGTCCCCGC CTCTGGGGCT GGGGAAGAAA TGCCCTGCTC GAGTTGCTTA CAAGCGCCTG 
GTTCGCATGA ACATGCCCAT CTCCAACGAG GACATGACTG TTCACTTCAC GTCCACGCTG 
ATGGCCCTCA TCCGGACGGC ACTGGAGATC AAGCTGGCCC CAGCTGGGAC AAAGCAGCAT 
CAGTGTGACG CGGAGTTGAG GAAGGAGATT TCCGTTGTGT GGGCCAATCT GCCCCAGAAG 
ACTTTGGACT TGCTGGTACC ACCCCATAAG CCTGATGAGA TGACAGTGGG GAAGGTTTAT 
GCAGCTCTGA TGATATTCGA CTTCTACAAG CAGAACAAAA CCACCAGAGA CCAGATGCAG 
CAGGCTCCTG GAGGCCTCTC CCAGATGGGT CCTGTGTCCC TGTTCCACCC TCTGAAGGCC 
ACCCTGGAGC AGACACAGCC GGCTGTGCTC CGAGGAGCCC GGGTTTTCCT TCGACAGAAG 
AGTTCCACCT CCCTCAGCAA TGGCGGGGCC ATACAAAACC AAGAGAGTGG CATCAAAGAG 
TCTGTCTCCT GGGGCACTCA AAGGACCCAG GATGCACCCC ATGAGGCCAG GCCACCCCTG 
GAGCGTGGCC ACTCCACAGA GATCCCTGTG GGGCGGTCAG GAGCACTGGC TGTGGACGTT 
CAGATGCAGA GCATAACCCG GAGGGGCCCT GATGGGGAGC CCCAGCCTGG GCTGGAGAGC 
CAGGGTCGAG CGGCCTCCAT GCCCCGCCTT GCGGCCGAGA CTCAGCCCGT CACAGATGCC 
AGCCCCATGA AGCGCTCCAT CTCCACGCTG GCCCAGCGGC CCCGTGGGAC TCATCTTTGC 
AGCACCACCC CGGACCGCCC ACCCCCTAGC CAGGCGTCGT CGCACCACCA CCACCACCGC 
TGCCACCGCC GCAGGGACAG GAAGCAGAGG TCCCTGGAGA AGGGGCCCAG CCTGTCTGCC 
GATATGGATG GCGCACCAAG CAGTGCTGTG GGGCCGGGGC TGCCCCCGGG AGAGGGGCCT 
ACAGGCTGCC GGCGGGAACG AGAGCGCCGG CAGGAGCGGG GCCGGTCCCA GGAGCGGAGG 
CAGCCCTCAT CCTCCTCCTC GGAGAAGCAG CGCTTCTACT CCTGCGACCG CTTTGGGGGC 
CGTGAGCCCC CGAAGCCCAA GCCCTCCCTC AGCAGCCACC CAACGTCGCC AACAGCTGGC 
CAGGAGCCGG GACCCCACCC ACAGGGCAGT GGTTCCGTGA ATGGGAGCCC CTTGCTGTCA 
ACATCTGGTG CTAGCACCCC CGGCCGCGGT GGGCGGAGGC AGCTCCCCCA GACGCCCCTG 
ACTCCCCGCC CCAGCATCAC CTACAAGACG GCCAACTCCT CACCCATCCA CTTCGCCGGG 
GCTCAGACCA GCCTCCCTGC CTTCTCCCCA GGCCGGCTCA GCCGTGGGCT TTCCGAACAC 
AACGCCCTGC TGCAGAGAGA CCCCCTCAGC CAGCCCCTGG CCCCTGGCTC TCGAATTGGC 
TCTGACCCTT ACCTGGGGCA GCGTCTGGAC AGTGAGGCCT CTGTCCACGC CCTGCCTGAG 
GACACTCTCA CTTTCGAGGA GGCTGTGGCC ACCAACTCGG GCCGCTCCTC CAGGACTTCC 
TACGTGTCCT CCCTGACCTC CCAGTCTCAC CCTCTCCGCC GCGTGCCCAA CGGTTACCAC 
TGCACCCTGG GACTCAGCTC GGGTGGCCGA GCACGGCACA GCTACCACCA CCCTGACCAA 
GACCACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517293.1
CDS	110..7144
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFVGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPIHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPGSRIGSDPYLGQRLDSEASVHALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_011518991.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518991.2

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGCAGTGCT GAAGAGAGCG 
GCCACCAAGA AGAGCAGAAA TGACCTGATC CACGCAGAGG AGGGAGAGGA CCGGTTTGCA 
GATCTCTGTG CTGTTGGATC CCCCTTCGCC CGCGCCAGCC TCAAGAGCGG GAAGACAGAG 
AGCTCGTCAT ACTTCCGGAG GAAGGAGAAG ATGTTCCGGT TTTTTATCCG GCGCATGGTG 
AAGGCTCAGA GCTTCTACTG GGTGGTGCTG TGCGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ATTACAACCA GCCGCGGCGG CTTACCACGA CCCTGTATTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGA 
AGCTACTTCC GGTCCTCCTT CAACTGCTTC GACTTTGGGG TCATCGTGGG GAGCGTCTTT 
GAAGTGGTCT GGGCGGCCAT CAAGCCGGGA AGCTCCTTTG GGATCAGTGT GCTGCGGGCC 
CTCCGCCTGC TGAGGATCTT CAAAGTCACG AAGTACTGGA GCTCCCTGCG GAACCTGGTG 
GTGTCCCTGC TGAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCTTGCT CTTCCTGTTC 
ATTGTGGTCT TCGCCCTGCT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTCCAGGAT 
GAGACTCCCA CAACCAACTT CGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC 
CTGACGGGAG AGGACTGGAA TGCAGTGATG TATCACGGGA TCGAATCGCA AGGCGGCGTC 
AGCAAAGGCA TGTTCTCGTC CTTTTACTTC ATTGTCCTGA CACTGTTCGG AAACTACACT 
CTGCTGAATG TCTTTCTGGC CATCGCTGTG GACAACCTGG CCAACGCCCA AGAGCTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA 
GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATCGCCGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTACG GCTGCAGAAC 
CTGCGGGCCA GCTGCGAGGC GCTGTACAGC GAGATGGACC CCGAGGAGCG GCTGCGCTTC 
GCCACTACGC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTGGTGGTG 
GAGCTGGGCC GCGACGGCGC GCGGGGGCCC GTGGGAGGCA AAGCCCGACC TGAGGCTGCG 
GAGGCCCCCG AGGGCGTCGA CCCTCCGCGC AGGCACCACC GGCACCGCGA CAAGGACAAG 
ACCCCCGCGG CGGGGGACCA GGACCGAGCA GAGGCCCCGA AGGCGGAGAG CGGGGAGCCC 
GGTGCCCGGG AGGAGCGGCC GCGGCCGCAC CGCAGCCACA GCAAGGAGGC CGCGGGGCCC 
CCGGAGGCGC GGAGCGAGCG CGGCCGAGGC CCAGGCCCCG AGGGCGGCCG GCGGCACCAC 
CGGCGCGGCT CCCCGGAGGA GGCGGCCGAG CGGGAGCCCC GACGCCACCG CGCGCACCGG 
CACCAGGATC CGAGCAAGGA GTGCGCCGGC GCCAAGGGCG AGCGGCGCGC GCGGCACCGC 
GGCGGCCCCC GAGCGGGGCC CCGGGAGGCG GAGAGCGGGG AGGAGCCGGC GCGGCGGCAC 
CGGGCCCGGC ACAAGGCGCA GCCTGCTCAC GAGGCTGTGG AGAAGGAGAC CACGGAGAAG 
GAGGCCACGG AGAAGGAGGC TGAGATAGTG GAAGCCGACA AGGAAAAGGA GCTCCGGAAC 
CACCAGCCCC GGGAGCCACA CTGTGACCTG GAGACCAGTG GGACTGTGAC TGTGGGTCCC 
ATGCACACAC TGCCCAGCAC CTGTCTCCAG AAGGTGGAGG AACAGCCAGA GGATGCAGAC 
AATCAGCGGA ACGTCACTCG CATGGGCAGT CAGCCCCCAG ACCCGAACAC TATTGTACAT 
ATCCCAGTGA TGCTGACGGG CCCTCTTGGG GAAGCCACGG TCGTTCCCAG TGGTAACGTG 
GACCTGGAAA GCCAAGCAGA GGGGAAGAAG GAGGTGGAAG CGGATGACGT GATGAGGAGC 
GGCCCCCGGC CTATCGTCCC ATACAGCTCC ATGTTCTGTT TAAGCCCCAC CAACCTGCTC 
CGCCGCTTCT GCCACTACAT CGTGACCATG AGGTACTTCG AGGTGGTCAT TCTCGTGGTC 
ATCGCCTTGA GCAGCATCGC CCTGGCTGCT GAGGACCCAG TGCGCACAGA CTCGCCCAGG 
AACAACGCTC TGAAATACCT GGATTACATT TTCACTGGTG TCTTTACCTT TGAGATGGTG 
ATAAAGATGA TCGACTTGGG ACTGCTGCTT CACCCTGGAG CCTATTTCCG GGACTTGTGG 
AACATTCTGG ACTTCATTGT GGTCAGTGGC GCCCTGGTGG CGTTTGCTTT CTCGAGCTTC 
GTGGGAGGAT CCAAAGGGAA AGACATCAAT ACCATCAAGT CTCTGAGAGT CCTTCGTGTC 
CTGCGGCCCC TCAAGACCAT CAAACGGCTG CCCAAGCTCA AGGCTGTGTT TGACTGTGTG 
GTGAACTCCC TGAAGAATGT CCTCAACATC TTGATTGTCT ACATGCTCTT CATGTTCATA 
TTTGCCGTCA TTGCGGTGCA GCTCTTCAAA GGGAAGTTTT TCTACTGCAC AGATGAATCC 
AAGGAGCTGG AGAGGGACTG CAGGGGTCAG TATTTGGATT ATGAGAAGGA GGAAGTGGAA 
GCTCAGCCCA GGCAGTGGAA GAAATACGAC TTTCACTACG ACAATGTGCT CTGGGCTCTG 
CTGACGCTGT TCACAGTGTC CACGGGAGAA GGCTGGCCCA TGGTGCTGAA ACACTCCGTG 
GATGCCACCT ATGAGGAGCA GGGTCCAAGC CCTGGGTACC GCATGGAGCT GTCCATCTTC 
TACGTGGTCT ACTTTGTGGT CTTTCCCTTC TTCTTCGTCA ACATCTTTGT GGCTTTGATC 
ATCATCACCT TCCAGGAGCA GGGGGACAAG GTGATGTCTG AATGCAGCCT GGAGAAGAAC 
GAGAGGGCTT GCATTGACTT CGCCATCAGC GCCAAACCCC TGACACGGTA CATGCCCCAA 
AACCGGCAGT CGTTCCAGTA TAAGACGTGG ACATTTGTGG TCTCCCCGCC CTTTGAATAC 
TTCATCATGG CCATGATAGC CCTCAACACT GTGGTGCTGA TGATGAAGTT CTATGATGCA 
CCCTATGAGT ACGAGCTGAT GCTGAAATGC CTGAACATCG TGTTCACATC CATGTTCTCC 
ATGGAATGCG TGCTGAAGAT CATCGCCTTT GGGGTGCTGA ACTATTTCAG AGATGCCTGG 
AATGTCTTTG ACTTTGTCAC TGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGAAACGA ACAATTTCAT CAACCTCAGC TTCCTCCGCC TCTTTCGAGC TGCGCGGCTG 
ATCAAGCTGC TCCGCCAGGG CTACACCATC CGCATCCTGC TGTGGACCTT TGTCCAGTCC 
TTCAAGGCCC TGCCCTACGT GTGTCTGCTC ATTGCCATGC TGTTCTTCAT CTACGCCATC 
ATCGGCATGC AGGTGTTTGG GAATATTGCC CTGGATGATG ACACCAGCAT CAACCGCCAC 
AACAACTTCC GGACGTTTTT GCAAGCCCTG ATGCTGCTGT TCAGGAGCGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCCTGCCTG AGCAACCAGG CCTGTGATGA GCAGGCCAAT 
GCCACCGAGT GTGGAAGTGA CTTTGCCTAC TTCTACTTCG TCTCCTTCAT CTTCCTGTGC 
TCCTTTCTGA TGTTGAACCT CTTTGTGGCT GTGATCATGG ACAATTTTGA GTACCTCACG 
CGGGACTCTT CCATCCTAGG TCCTCACCAC TTGGATGAGT TCATCCGGGT CTGGGCTGAA 
TACGACCCGG CTGCGTGTGG GCGCATCAGT TACAATGACA TGTTTGAGAT GCTGAAACAC 
ATGTCCCCGC CTCTGGGGCT GGGGAAGAAA TGCCCTGCTC GAGTTGCTTA CAAGCGCCTG 
GTTCGCATGA ACATGCCCAT CTCCAACGAG GACATGACTG TTCACTTCAC GTCCACGCTG 
ATGGCCCTCA TCCGGACGGC ACTGGAGATC AAGCTGGCCC CAGCTGGGAC AAAGCAGCAT 
CAGTGTGACG CGGAGTTGAG GAAGGAGATT TCCGTTGTGT GGGCCAATCT GCCCCAGAAG 
ACTTTGGACT TGCTGGTACC ACCCCATAAG CCTGATGAGA TGACAGTGGG GAAGGTTTAT 
GCAGCTCTGA TGATATTCGA CTTCTACAAG CAGAACAAAA CCACCAGAGA CCAGATGCAG 
CAGGCTCCTG GAGGCCTCTC CCAGATGGGT CCTGTGTCCC TGTTCCACCC TCTGAAGGCC 
ACCCTGGAGC AGACACAGCC GGCTGTGCTC CGAGGAGCCC GGGTTTTCCT TCGACAGAAG 
AGTTCCACCT CCCTCAGCAA TGGCGGGGCC ATACAAAACC AAGAGAGTGG CATCAAAGAG 
TCTGTCTCCT GGGGCACTCA AAGGACCCAG GATGCACCCC ATGAGGCCAG GCCACCCCTG 
GAGCGTGGCC ACTCCACAGA GATCCCTGTG GGGCGGTCAG GAGCACTGGC TGTGGACGTT 
CAGATGCAGA GCATAACCCG GAGGGGCCCT GATGGGGAGC CCCAGCCTGG GCTGGAGAGC 
CAGGGTCGAG CGGCCTCCAT GCCCCGCCTT GCGGCCGAGA CTCAGCCCGT CACAGATGCC 
AGCCCCATGA AGCGCTCCAT CTCCACGCTG GCCCAGCGGC CCCGTGGGAC TCATCTTTGC 
AGCACCACCC CGGACCGCCC ACCCCCTAGC CAGGCGTCGT CGCACCACCA CCACCACCGC 
TGCCACCGCC GCAGGGACAG GAAGCAGAGG TCCCTGGAGA AGGGGCCCAG CCTGTCTGCC 
GATATGGATG GCGCACCAAG CAGTGCTGTG GGGCCGGGGC TGCCCCCGGG AGAGGGGCCT 
ACAGGCTGCC GGCGGGAACG AGAGCGCCGG CAGGAGCGGG GCCGGTCCCA GGAGCGGAGG 
CAGCCCTCAT CCTCCTCCTC GGAGAAGCAG CGCTTCTACT CCTGCGACCG CTTTGGGGGC 
CGTGAGCCCC CGAAGCCCAA GCCCTCCCTC AGCAGCCACC CAACGTCGCC AACAGCTGGC 
CAGGAGCCGG GACCCCACCC ACAGGGCAGT GGTTCCGTGA ATGGGAGCCC CTTGCTGTCA 
ACATCTGGTG CTAGCACCCC CGGCCGCGGT GGGCGGAGGC AGCTCCCCCA GACGCCCCTG 
ACTCCCCGCC CCAGCATCAC CTACAAGACG GCCAACTCCT CACCCATCCA CTTCGCCGGG 
GCTCAGACCA GCCTCCCTGC CTTCTCCCCA GGCCGGCTCA GCCGTGGGCT TTCCGAACAC 
AACGCCCTGC TGCAGAGAGA CCCCCTCAGC CAGCCCCTGG CCCCTGGCTC TCGAATTGGC 
TCTGACCCTT ACCTGGGGCA GCGTCTGGAC AGTGAGGCCT CTGTCCACGC CCTGCCTGAG 
GACACTCTCA CTTTCGAGGA GGCTGTGGCC ACCAACTCGG GCCGCTCCTC CAGGACTTCC 
TACGTGTCCT CCCTGACCTC CCAGTCTCAC CCTCTCCGCC GCGTGCCCAA CGGTTACCAC 
TGCACCCTGG GACTCAGCTC GGGTGGCCGA GCACGGCACA GCTACCACCA CCCTGACCAA 
GACCACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_011518992.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517294.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X3, mRNA.

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CloneID	OHu56563
Clone ID Related Accession (Same CDS sequence)	XM_011518992.2
Accession Version	XM_011518992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGCAGTGCT GAAGAGAGCG 
GCCACCAAGA AGAGCAGAAA TGACCTGATC CACGCAGAGG AGGGAGAGGA CCGGTTTGCA 
GATCTCTGTG CTGTTGGATC CCCCTTCGCC CGCGCCAGCC TCAAGAGCGG GAAGACAGAG 
AGCTCGTCAT ACTTCCGGAG GAAGGAGAAG ATGTTCCGGT TTTTTATCCG GCGCATGGTG 
AAGGCTCAGA GCTTCTACTG GGTGGTGCTG TGCGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ATTACAACCA GCCGCGGCGG CTTACCACGA CCCTGTATTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGA 
AGCTACTTCC GGTCCTCCTT CAACTGCTTC GACTTTGGGG TCATCGTGGG GAGCGTCTTT 
GAAGTGGTCT GGGCGGCCAT CAAGCCGGGA AGCTCCTTTG GGATCAGTGT GCTGCGGGCC 
CTCCGCCTGC TGAGGATCTT CAAAGTCACG AAGTACTGGA GCTCCCTGCG GAACCTGGTG 
GTGTCCCTGC TGAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCTTGCT CTTCCTGTTC 
ATTGTGGTCT TCGCCCTGCT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTCCAGGAT 
GAGACTCCCA CAACCAACTT CGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC 
CTGACGGGAG AGGACTGGAA TGCAGTGATG TATCACGGGA TCGAATCGCA AGGCGGCGTC 
AGCAAAGGCA TGTTCTCGTC CTTTTACTTC ATTGTCCTGA CACTGTTCGG AAACTACACT 
CTGCTGAATG TCTTTCTGGC CATCGCTGTG GACAACCTGG CCAACGCCCA AGAGCTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA 
GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATCGCCGC GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC GAGCTTCGTG 
GGAGGATCCA AAGGGAAAGA CATCAATACC ATCAAGTCTC TGAGAGTCCT TCGTGTCCTG 
CGGCCCCTCA AGACCATCAA ACGGCTGCCC AAGCTCAAGG CTGTGTTTGA CTGTGTGGTG 
AACTCCCTGA AGAATGTCCT CAACATCTTG ATTGTCTACA TGCTCTTCAT GTTCATATTT 
GCCGTCATTG CGGTGCAGCT CTTCAAAGGG AAGTTTTTCT ACTGCACAGA TGAATCCAAG 
GAGCTGGAGA GGGACTGCAG GGGTCAGTAT TTGGATTATG AGAAGGAGGA AGTGGAAGCT 
CAGCCCAGGC AGTGGAAGAA ATACGACTTT CACTACGACA ATGTGCTCTG GGCTCTGCTG 
ACGCTGTTCA CAGTGTCCAC GGGAGAAGGC TGGCCCATGG TGCTGAAACA CTCCGTGGAT 
GCCACCTATG AGGAGCAGGG TCCAAGCCCT GGGTACCGCA TGGAGCTGTC CATCTTCTAC 
GTGGTCTACT TTGTGGTCTT TCCCTTCTTC TTCGTCAACA TCTTTGTGGC TTTGATCATC 
ATCACCTTCC AGGAGCAGGG GGACAAGGTG ATGTCTGAAT GCAGCCTGGA GAAGAACGAG 
AGGGCTTGCA TTGACTTCGC CATCAGCGCC AAACCCCTGA CACGGTACAT GCCCCAAAAC 
CGGCAGTCGT TCCAGTATAA GACGTGGACA TTTGTGGTCT CCCCGCCCTT TGAATACTTC 
ATCATGGCCA TGATAGCCCT CAACACTGTG GTGCTGATGA TGAAGTTCTA TGATGCACCC 
TATGAGTACG AGCTGATGCT GAAATGCCTG AACATCGTGT TCACATCCAT GTTCTCCATG 
GAATGCGTGC TGAAGATCAT CGCCTTTGGG GTGCTGAACT ATTTCAGAGA TGCCTGGAAT 
GTCTTTGACT TTGTCACTGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GAAACGAACA ATTTCATCAA CCTCAGCTTC CTCCGCCTCT TTCGAGCTGC GCGGCTGATC 
AAGCTGCTCC GCCAGGGCTA CACCATCCGC ATCCTGCTGT GGACCTTTGT CCAGTCCTTC 
AAGGCCCTGC CCTACGTGTG TCTGCTCATT GCCATGCTGT TCTTCATCTA CGCCATCATC 
GGCATGCAGG TGTTTGGGAA TATTGCCCTG GATGATGACA CCAGCATCAA CCGCCACAAC 
AACTTCCGGA CGTTTTTGCA AGCCCTGATG CTGCTGTTCA GGAGCGCCAC GGGGGAGGCC 
TGGCACGAGA TCATGCTGTC CTGCCTGAGC AACCAGGCCT GTGATGAGCA GGCCAATGCC 
ACCGAGTGTG GAAGTGACTT TGCCTACTTC TACTTCGTCT CCTTCATCTT CCTGTGCTCC 
TTTCTGATGT TGAACCTCTT TGTGGCTGTG ATCATGGACA ATTTTGAGTA CCTCACGCGG 
GACTCTTCCA TCCTAGGTCC TCACCACTTG GATGAGTTCA TCCGGGTCTG GGCTGAATAC 
GACCCGGCTG CGTGTGGGCG CATCAGTTAC AATGACATGT TTGAGATGCT GAAACACATG 
TCCCCGCCTC TGGGGCTGGG GAAGAAATGC CCTGCTCGAG TTGCTTACAA GCGCCTGGTT 
CGCATGAACA TGCCCATCTC CAACGAGGAC ATGACTGTTC ACTTCACGTC CACGCTGATG 
GCCCTCATCC GGACGGCACT GGAGATCAAG CTGGCCCCAG CTGGGACAAA GCAGCATCAG 
TGTGACGCGG AGTTGAGGAA GGAGATTTCC GTTGTGTGGG CCAATCTGCC CCAGAAGACT 
TTGGACTTGC TGGTACCACC CCATAAGCCT GATGAGATGA CAGTGGGGAA GGTTTATGCA 
GCTCTGATGA TATTCGACTT CTACAAGCAG AACAAAACCA CCAGAGACCA GATGCAGCAG 
GCTCCTGGAG GCCTCTCCCA GATGGGTCCT GTGTCCCTGT TCCACCCTCT GAAGGCCACC 
CTGGAGCAGA CACAGCCGGC TGTGCTCCGA GGAGCCCGGG TTTTCCTTCG ACAGAAGAGT 
TCCACCTCCC TCAGCAATGG CGGGGCCATA CAAAACCAAG AGAGTGGCAT CAAAGAGTCT 
GTCTCCTGGG GCACTCAAAG GACCCAGGAT GCACCCCATG AGGCCAGGCC ACCCCTGGAG 
CGTGGCCACT CCACAGAGAT CCCTGTGGGG CGGTCAGGAG CACTGGCTGT GGACGTTCAG 
ATGCAGAGCA TAACCCGGAG GGGCCCTGAT GGGGAGCCCC AGCCTGGGCT GGAGAGCCAG 
GGTCGAGCGG CCTCCATGCC CCGCCTTGCG GCCGAGACTC AGCCCGTCAC AGATGCCAGC 
CCCATGAAGC GCTCCATCTC CACGCTGGCC CAGCGGCCCC GTGGGACTCA TCTTTGCAGC 
ACCACCCCGG ACCGCCCACC CCCTAGCCAG GCGTCGTCGC ACCACCACCA CCACCGCTGC 
CACCGCCGCA GGGACAGGAA GCAGAGGTCC CTGGAGAAGG GGCCCAGCCT GTCTGCCGAT 
ATGGATGGCG CACCAAGCAG TGCTGTGGGG CCGGGGCTGC CCCCGGGAGA GGGGCCTACA 
GGCTGCCGGC GGGAACGAGA GCGCCGGCAG GAGCGGGGCC GGTCCCAGGA GCGGAGGCAG 
CCCTCATCCT CCTCCTCGGA GAAGCAGCGC TTCTACTCCT GCGACCGCTT TGGGGGCCGT 
GAGCCCCCGA AGCCCAAGCC CTCCCTCAGC AGCCACCCAA CGTCGCCAAC AGCTGGCCAG 
GAGCCGGGAC CCCACCCACA GGGCAGTGGT TCCGTGAATG GGAGCCCCTT GCTGTCAACA 
TCTGGTGCTA GCACCCCCGG CCGCGGTGGG CGGAGGCAGC TCCCCCAGAC GCCCCTGACT 
CCCCGCCCCA GCATCACCTA CAAGACGGCC AACTCCTCAC CCATCCACTT CGCCGGGGCT 
CAGACCAGCC TCCCTGCCTT CTCCCCAGGC CGGCTCAGCC GTGGGCTTTC CGAACACAAC 
GCCCTGCTGC AGAGAGACCC CCTCAGCCAG CCCCTGGCCC CTGGCTCTCG AATTGGCTCT 
GACCCTTACC TGGGGCAGCG TCTGGACAGT GAGGCCTCTG TCCACGCCCT GCCTGAGGAC 
ACTCTCACTT TCGAGGAGGC TGTGGCCACC AACTCGGGCC GCTCCTCCAG GACTTCCTAC 
GTGTCCTCCC TGACCTCCCA GTCTCACCCT CTCCGCCGCG TGCCCAACGG TTACCACTGC 
ACCCTGGGAC TCAGCTCGGG TGGCCGAGCA CGGCACAGCT ACCACCACCC TGACCAAGAC 
CACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517294.1
CDS	110..7141
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFVGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPIHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPGSRIGSDPYLGQRLDSEASVHALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_011518992.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518992.2

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGCAGTGCT GAAGAGAGCG 
GCCACCAAGA AGAGCAGAAA TGACCTGATC CACGCAGAGG AGGGAGAGGA CCGGTTTGCA 
GATCTCTGTG CTGTTGGATC CCCCTTCGCC CGCGCCAGCC TCAAGAGCGG GAAGACAGAG 
AGCTCGTCAT ACTTCCGGAG GAAGGAGAAG ATGTTCCGGT TTTTTATCCG GCGCATGGTG 
AAGGCTCAGA GCTTCTACTG GGTGGTGCTG TGCGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ATTACAACCA GCCGCGGCGG CTTACCACGA CCCTGTATTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGA 
AGCTACTTCC GGTCCTCCTT CAACTGCTTC GACTTTGGGG TCATCGTGGG GAGCGTCTTT 
GAAGTGGTCT GGGCGGCCAT CAAGCCGGGA AGCTCCTTTG GGATCAGTGT GCTGCGGGCC 
CTCCGCCTGC TGAGGATCTT CAAAGTCACG AAGTACTGGA GCTCCCTGCG GAACCTGGTG 
GTGTCCCTGC TGAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCTTGCT CTTCCTGTTC 
ATTGTGGTCT TCGCCCTGCT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTCCAGGAT 
GAGACTCCCA CAACCAACTT CGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC 
CTGACGGGAG AGGACTGGAA TGCAGTGATG TATCACGGGA TCGAATCGCA AGGCGGCGTC 
AGCAAAGGCA TGTTCTCGTC CTTTTACTTC ATTGTCCTGA CACTGTTCGG AAACTACACT 
CTGCTGAATG TCTTTCTGGC CATCGCTGTG GACAACCTGG CCAACGCCCA AGAGCTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA 
GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATCGCCGC GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC GAGCTTCGTG 
GGAGGATCCA AAGGGAAAGA CATCAATACC ATCAAGTCTC TGAGAGTCCT TCGTGTCCTG 
CGGCCCCTCA AGACCATCAA ACGGCTGCCC AAGCTCAAGG CTGTGTTTGA CTGTGTGGTG 
AACTCCCTGA AGAATGTCCT CAACATCTTG ATTGTCTACA TGCTCTTCAT GTTCATATTT 
GCCGTCATTG CGGTGCAGCT CTTCAAAGGG AAGTTTTTCT ACTGCACAGA TGAATCCAAG 
GAGCTGGAGA GGGACTGCAG GGGTCAGTAT TTGGATTATG AGAAGGAGGA AGTGGAAGCT 
CAGCCCAGGC AGTGGAAGAA ATACGACTTT CACTACGACA ATGTGCTCTG GGCTCTGCTG 
ACGCTGTTCA CAGTGTCCAC GGGAGAAGGC TGGCCCATGG TGCTGAAACA CTCCGTGGAT 
GCCACCTATG AGGAGCAGGG TCCAAGCCCT GGGTACCGCA TGGAGCTGTC CATCTTCTAC 
GTGGTCTACT TTGTGGTCTT TCCCTTCTTC TTCGTCAACA TCTTTGTGGC TTTGATCATC 
ATCACCTTCC AGGAGCAGGG GGACAAGGTG ATGTCTGAAT GCAGCCTGGA GAAGAACGAG 
AGGGCTTGCA TTGACTTCGC CATCAGCGCC AAACCCCTGA CACGGTACAT GCCCCAAAAC 
CGGCAGTCGT TCCAGTATAA GACGTGGACA TTTGTGGTCT CCCCGCCCTT TGAATACTTC 
ATCATGGCCA TGATAGCCCT CAACACTGTG GTGCTGATGA TGAAGTTCTA TGATGCACCC 
TATGAGTACG AGCTGATGCT GAAATGCCTG AACATCGTGT TCACATCCAT GTTCTCCATG 
GAATGCGTGC TGAAGATCAT CGCCTTTGGG GTGCTGAACT ATTTCAGAGA TGCCTGGAAT 
GTCTTTGACT TTGTCACTGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GAAACGAACA ATTTCATCAA CCTCAGCTTC CTCCGCCTCT TTCGAGCTGC GCGGCTGATC 
AAGCTGCTCC GCCAGGGCTA CACCATCCGC ATCCTGCTGT GGACCTTTGT CCAGTCCTTC 
AAGGCCCTGC CCTACGTGTG TCTGCTCATT GCCATGCTGT TCTTCATCTA CGCCATCATC 
GGCATGCAGG TGTTTGGGAA TATTGCCCTG GATGATGACA CCAGCATCAA CCGCCACAAC 
AACTTCCGGA CGTTTTTGCA AGCCCTGATG CTGCTGTTCA GGAGCGCCAC GGGGGAGGCC 
TGGCACGAGA TCATGCTGTC CTGCCTGAGC AACCAGGCCT GTGATGAGCA GGCCAATGCC 
ACCGAGTGTG GAAGTGACTT TGCCTACTTC TACTTCGTCT CCTTCATCTT CCTGTGCTCC 
TTTCTGATGT TGAACCTCTT TGTGGCTGTG ATCATGGACA ATTTTGAGTA CCTCACGCGG 
GACTCTTCCA TCCTAGGTCC TCACCACTTG GATGAGTTCA TCCGGGTCTG GGCTGAATAC 
GACCCGGCTG CGTGTGGGCG CATCAGTTAC AATGACATGT TTGAGATGCT GAAACACATG 
TCCCCGCCTC TGGGGCTGGG GAAGAAATGC CCTGCTCGAG TTGCTTACAA GCGCCTGGTT 
CGCATGAACA TGCCCATCTC CAACGAGGAC ATGACTGTTC ACTTCACGTC CACGCTGATG 
GCCCTCATCC GGACGGCACT GGAGATCAAG CTGGCCCCAG CTGGGACAAA GCAGCATCAG 
TGTGACGCGG AGTTGAGGAA GGAGATTTCC GTTGTGTGGG CCAATCTGCC CCAGAAGACT 
TTGGACTTGC TGGTACCACC CCATAAGCCT GATGAGATGA CAGTGGGGAA GGTTTATGCA 
GCTCTGATGA TATTCGACTT CTACAAGCAG AACAAAACCA CCAGAGACCA GATGCAGCAG 
GCTCCTGGAG GCCTCTCCCA GATGGGTCCT GTGTCCCTGT TCCACCCTCT GAAGGCCACC 
CTGGAGCAGA CACAGCCGGC TGTGCTCCGA GGAGCCCGGG TTTTCCTTCG ACAGAAGAGT 
TCCACCTCCC TCAGCAATGG CGGGGCCATA CAAAACCAAG AGAGTGGCAT CAAAGAGTCT 
GTCTCCTGGG GCACTCAAAG GACCCAGGAT GCACCCCATG AGGCCAGGCC ACCCCTGGAG 
CGTGGCCACT CCACAGAGAT CCCTGTGGGG CGGTCAGGAG CACTGGCTGT GGACGTTCAG 
ATGCAGAGCA TAACCCGGAG GGGCCCTGAT GGGGAGCCCC AGCCTGGGCT GGAGAGCCAG 
GGTCGAGCGG CCTCCATGCC CCGCCTTGCG GCCGAGACTC AGCCCGTCAC AGATGCCAGC 
CCCATGAAGC GCTCCATCTC CACGCTGGCC CAGCGGCCCC GTGGGACTCA TCTTTGCAGC 
ACCACCCCGG ACCGCCCACC CCCTAGCCAG GCGTCGTCGC ACCACCACCA CCACCGCTGC 
CACCGCCGCA GGGACAGGAA GCAGAGGTCC CTGGAGAAGG GGCCCAGCCT GTCTGCCGAT 
ATGGATGGCG CACCAAGCAG TGCTGTGGGG CCGGGGCTGC CCCCGGGAGA GGGGCCTACA 
GGCTGCCGGC GGGAACGAGA GCGCCGGCAG GAGCGGGGCC GGTCCCAGGA GCGGAGGCAG 
CCCTCATCCT CCTCCTCGGA GAAGCAGCGC TTCTACTCCT GCGACCGCTT TGGGGGCCGT 
GAGCCCCCGA AGCCCAAGCC CTCCCTCAGC AGCCACCCAA CGTCGCCAAC AGCTGGCCAG 
GAGCCGGGAC CCCACCCACA GGGCAGTGGT TCCGTGAATG GGAGCCCCTT GCTGTCAACA 
TCTGGTGCTA GCACCCCCGG CCGCGGTGGG CGGAGGCAGC TCCCCCAGAC GCCCCTGACT 
CCCCGCCCCA GCATCACCTA CAAGACGGCC AACTCCTCAC CCATCCACTT CGCCGGGGCT 
CAGACCAGCC TCCCTGCCTT CTCCCCAGGC CGGCTCAGCC GTGGGCTTTC CGAACACAAC 
GCCCTGCTGC AGAGAGACCC CCTCAGCCAG CCCCTGGCCC CTGGCTCTCG AATTGGCTCT 
GACCCTTACC TGGGGCAGCG TCTGGACAGT GAGGCCTCTG TCCACGCCCT GCCTGAGGAC 
ACTCTCACTT TCGAGGAGGC TGTGGCCACC AACTCGGGCC GCTCCTCCAG GACTTCCTAC 
GTGTCCTCCC TGACCTCCCA GTCTCACCCT CTCCGCCGCG TGCCCAACGG TTACCACTGC 
ACCCTGGGAC TCAGCTCGGG TGGCCGAGCA CGGCACAGCT ACCACCACCC TGACCAAGAC 
CACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_011518993.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517295.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X5, mRNA.

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CloneID	OHu56564
Clone ID Related Accession (Same CDS sequence)	XM_011518993.2
Accession Version	XM_011518993.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC GAGCTTCGTG 
GGAGGATCCA AAGGGAAAGA CATCAATACC ATCAAGTCTC TGAGAGTCCT TCGTGTCCTG 
CGGCCCCTCA AGACCATCAA ACGGCTGCCC AAGCTCAAGG CTGTGTTTGA CTGTGTGGTG 
AACTCCCTGA AGAATGTCCT CAACATCTTG ATTGTCTACA TGCTCTTCAT GTTCATATTT 
GCCGTCATTG CGGTGCAGCT CTTCAAAGGG AAGTTTTTCT ACTGCACAGA TGAATCCAAG 
GAGCTGGAGA GGGACTGCAG GGGTCAGTAT TTGGATTATG AGAAGGAGGA AGTGGAAGCT 
CAGCCCAGGC AGTGGAAGAA ATACGACTTT CACTACGACA ATGTGCTCTG GGCTCTGCTG 
ACGCTGTTCA CAGTGTCCAC GGGAGAAGGC TGGCCCATGG TGCTGAAACA CTCCGTGGAT 
GCCACCTATG AGGAGCAGGG TCCAAGCCCT GGGTACCGCA TGGAGCTGTC CATCTTCTAC 
GTGGTCTACT TTGTGGTCTT TCCCTTCTTC TTCGTCAACA TCTTTGTGGC TTTGATCATC 
ATCACCTTCC AGGAGCAGGG GGACAAGGTG ATGTCTGAAT GCAGCCTGGA GAAGAACGAG 
AGGGCTTGCA TTGACTTCGC CATCAGCGCC AAACCCCTGA CACGGTACAT GCCCCAAAAC 
CGGCAGTCGT TCCAGTATAA GACGTGGACA TTTGTGGTCT CCCCGCCCTT TGAATACTTC 
ATCATGGCCA TGATAGCCCT CAACACTGTG GTGCTGATGA TGAAGTTCTA TGATGCACCC 
TATGAGTACG AGCTGATGCT GAAATGCCTG AACATCGTGT TCACATCCAT GTTCTCCATG 
GAATGCGTGC TGAAGATCAT CGCCTTTGGG GTGCTGAACT ATTTCAGAGA TGCCTGGAAT 
GTCTTTGACT TTGTCACTGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
AACAATTTCA TCAACCTCAG CTTCCTCCGC CTCTTTCGAG CTGCGCGGCT GATCAAGCTG 
CTCCGCCAGG GCTACACCAT CCGCATCCTG CTGTGGACCT TTGTCCAGTC CTTCAAGGCC 
CTGCCCTACG TGTGTCTGCT CATTGCCATG CTGTTCTTCA TCTACGCCAT CATCGGCATG 
CAGGTGTTTG GGAATATTGC CCTGGATGAT GACACCAGCA TCAACCGCCA CAACAACTTC 
CGGACGTTTT TGCAAGCCCT GATGCTGCTG TTCAGGAGCG CCACGGGGGA GGCCTGGCAC 
GAGATCATGC TGTCCTGCCT GAGCAACCAG GCCTGTGATG AGCAGGCCAA TGCCACCGAG 
TGTGGAAGTG ACTTTGCCTA CTTCTACTTC GTCTCCTTCA TCTTCCTGTG CTCCTTTCTG 
ATGTTGAACC TCTTTGTGGC TGTGATCATG GACAATTTTG AGTACCTCAC GCGGGACTCT 
TCCATCCTAG GTCCTCACCA CTTGGATGAG TTCATCCGGG TCTGGGCTGA ATACGACCCG 
GCTGCGTGTG GGCGCATCAG TTACAATGAC ATGTTTGAGA TGCTGAAACA CATGTCCCCG 
CCTCTGGGGC TGGGGAAGAA ATGCCCTGCT CGAGTTGCTT ACAAGCGCCT GGTTCGCATG 
AACATGCCCA TCTCCAACGA GGACATGACT GTTCACTTCA CGTCCACGCT GATGGCCCTC 
ATCCGGACGG CACTGGAGAT CAAGCTGGCC CCAGCTGGGA CAAAGCAGCA TCAGTGTGAC 
GCGGAGTTGA GGAAGGAGAT TTCCGTTGTG TGGGCCAATC TGCCCCAGAA GACTTTGGAC 
TTGCTGGTAC CACCCCATAA GCCTGATGAG ATGACAGTGG GGAAGGTTTA TGCAGCTCTG 
ATGATATTCG ACTTCTACAA GCAGAACAAA ACCACCAGAG ACCAGATGCA GCAGGCTCCT 
GGAGGCCTCT CCCAGATGGG TCCTGTGTCC CTGTTCCACC CTCTGAAGGC CACCCTGGAG 
CAGACACAGC CGGCTGTGCT CCGAGGAGCC CGGGTTTTCC TTCGACAGAA GAGTTCCACC 
TCCCTCAGCA ATGGCGGGGC CATACAAAAC CAAGAGAGTG GCATCAAAGA GTCTGTCTCC 
TGGGGCACTC AAAGGACCCA GGATGCACCC CATGAGGCCA GGCCACCCCT GGAGCGTGGC 
CACTCCACAG AGATCCCTGT GGGGCGGTCA GGAGCACTGG CTGTGGACGT TCAGATGCAG 
AGCATAACCC GGAGGGGCCC TGATGGGGAG CCCCAGCCTG GGCTGGAGAG CCAGGGTCGA 
GCGGCCTCCA TGCCCCGCCT TGCGGCCGAG ACTCAGCCCG TCACAGATGC CAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GGCCCAGCGG CCCCGTGGGA CTCATCTTTG CAGCACCACC 
CCGGACCGCC CACCCCCTAG CCAGGCGTCG TCGCACCACC ACCACCACCG CTGCCACCGC 
CGCAGGGACA GGAAGCAGAG GTCCCTGGAG AAGGGGCCCA GCCTGTCTGC CGATATGGAT 
GGCGCACCAA GCAGTGCTGT GGGGCCGGGG CTGCCCCCGG GAGAGGGGCC TACAGGCTGC 
CGGCGGGAAC GAGAGCGCCG GCAGGAGCGG GGCCGGTCCC AGGAGCGGAG GCAGCCCTCA 
TCCTCCTCCT CGGAGAAGCA GCGCTTCTAC TCCTGCGACC GCTTTGGGGG CCGTGAGCCC 
CCGAAGCCCA AGCCCTCCCT CAGCAGCCAC CCAACGTCGC CAACAGCTGG CCAGGAGCCG 
GGACCCCACC CACAGGGCAG TGGTTCCGTG AATGGGAGCC CCTTGCTGTC AACATCTGGT 
GCTAGCACCC CCGGCCGCGG TGGGCGGAGG CAGCTCCCCC AGACGCCCCT GACTCCCCGC 
CCCAGCATCA CCTACAAGAC GGCCAACTCC TCACCCATCC ACTTCGCCGG GGCTCAGACC 
AGCCTCCCTG CCTTCTCCCC AGGCCGGCTC AGCCGTGGGC TTTCCGAACA CAACGCCCTG 
CTGCAGAGAG ACCCCCTCAG CCAGCCCCTG GCCCCTGGCT CTCGAATTGG CTCTGACCCT 
TACCTGGGGC AGCGTCTGGA CAGTGAGGCC TCTGTCCACG CCCTGCCTGA GGACACTCTC 
ACTTTCGAGG AGGCTGTGGC CACCAACTCG GGCCGCTCCT CCAGGACTTC CTACGTGTCC 
TCCCTGACCT CCCAGTCTCA CCCTCTCCGC CGCGTGCCCA ACGGTTACCA CTGCACCCTG 
GGACTCAGCT CGGGTGGCCG AGCACGGCAC AGCTACCACC ACCCTGACCA AGACCACTGG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517295.1
CDS	109..7134
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFVGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPIHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPGSRIGSDPYLGQRLDSEASVHALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_011518993.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518993.2

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC GAGCTTCGTG 
GGAGGATCCA AAGGGAAAGA CATCAATACC ATCAAGTCTC TGAGAGTCCT TCGTGTCCTG 
CGGCCCCTCA AGACCATCAA ACGGCTGCCC AAGCTCAAGG CTGTGTTTGA CTGTGTGGTG 
AACTCCCTGA AGAATGTCCT CAACATCTTG ATTGTCTACA TGCTCTTCAT GTTCATATTT 
GCCGTCATTG CGGTGCAGCT CTTCAAAGGG AAGTTTTTCT ACTGCACAGA TGAATCCAAG 
GAGCTGGAGA GGGACTGCAG GGGTCAGTAT TTGGATTATG AGAAGGAGGA AGTGGAAGCT 
CAGCCCAGGC AGTGGAAGAA ATACGACTTT CACTACGACA ATGTGCTCTG GGCTCTGCTG 
ACGCTGTTCA CAGTGTCCAC GGGAGAAGGC TGGCCCATGG TGCTGAAACA CTCCGTGGAT 
GCCACCTATG AGGAGCAGGG TCCAAGCCCT GGGTACCGCA TGGAGCTGTC CATCTTCTAC 
GTGGTCTACT TTGTGGTCTT TCCCTTCTTC TTCGTCAACA TCTTTGTGGC TTTGATCATC 
ATCACCTTCC AGGAGCAGGG GGACAAGGTG ATGTCTGAAT GCAGCCTGGA GAAGAACGAG 
AGGGCTTGCA TTGACTTCGC CATCAGCGCC AAACCCCTGA CACGGTACAT GCCCCAAAAC 
CGGCAGTCGT TCCAGTATAA GACGTGGACA TTTGTGGTCT CCCCGCCCTT TGAATACTTC 
ATCATGGCCA TGATAGCCCT CAACACTGTG GTGCTGATGA TGAAGTTCTA TGATGCACCC 
TATGAGTACG AGCTGATGCT GAAATGCCTG AACATCGTGT TCACATCCAT GTTCTCCATG 
GAATGCGTGC TGAAGATCAT CGCCTTTGGG GTGCTGAACT ATTTCAGAGA TGCCTGGAAT 
GTCTTTGACT TTGTCACTGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
AACAATTTCA TCAACCTCAG CTTCCTCCGC CTCTTTCGAG CTGCGCGGCT GATCAAGCTG 
CTCCGCCAGG GCTACACCAT CCGCATCCTG CTGTGGACCT TTGTCCAGTC CTTCAAGGCC 
CTGCCCTACG TGTGTCTGCT CATTGCCATG CTGTTCTTCA TCTACGCCAT CATCGGCATG 
CAGGTGTTTG GGAATATTGC CCTGGATGAT GACACCAGCA TCAACCGCCA CAACAACTTC 
CGGACGTTTT TGCAAGCCCT GATGCTGCTG TTCAGGAGCG CCACGGGGGA GGCCTGGCAC 
GAGATCATGC TGTCCTGCCT GAGCAACCAG GCCTGTGATG AGCAGGCCAA TGCCACCGAG 
TGTGGAAGTG ACTTTGCCTA CTTCTACTTC GTCTCCTTCA TCTTCCTGTG CTCCTTTCTG 
ATGTTGAACC TCTTTGTGGC TGTGATCATG GACAATTTTG AGTACCTCAC GCGGGACTCT 
TCCATCCTAG GTCCTCACCA CTTGGATGAG TTCATCCGGG TCTGGGCTGA ATACGACCCG 
GCTGCGTGTG GGCGCATCAG TTACAATGAC ATGTTTGAGA TGCTGAAACA CATGTCCCCG 
CCTCTGGGGC TGGGGAAGAA ATGCCCTGCT CGAGTTGCTT ACAAGCGCCT GGTTCGCATG 
AACATGCCCA TCTCCAACGA GGACATGACT GTTCACTTCA CGTCCACGCT GATGGCCCTC 
ATCCGGACGG CACTGGAGAT CAAGCTGGCC CCAGCTGGGA CAAAGCAGCA TCAGTGTGAC 
GCGGAGTTGA GGAAGGAGAT TTCCGTTGTG TGGGCCAATC TGCCCCAGAA GACTTTGGAC 
TTGCTGGTAC CACCCCATAA GCCTGATGAG ATGACAGTGG GGAAGGTTTA TGCAGCTCTG 
ATGATATTCG ACTTCTACAA GCAGAACAAA ACCACCAGAG ACCAGATGCA GCAGGCTCCT 
GGAGGCCTCT CCCAGATGGG TCCTGTGTCC CTGTTCCACC CTCTGAAGGC CACCCTGGAG 
CAGACACAGC CGGCTGTGCT CCGAGGAGCC CGGGTTTTCC TTCGACAGAA GAGTTCCACC 
TCCCTCAGCA ATGGCGGGGC CATACAAAAC CAAGAGAGTG GCATCAAAGA GTCTGTCTCC 
TGGGGCACTC AAAGGACCCA GGATGCACCC CATGAGGCCA GGCCACCCCT GGAGCGTGGC 
CACTCCACAG AGATCCCTGT GGGGCGGTCA GGAGCACTGG CTGTGGACGT TCAGATGCAG 
AGCATAACCC GGAGGGGCCC TGATGGGGAG CCCCAGCCTG GGCTGGAGAG CCAGGGTCGA 
GCGGCCTCCA TGCCCCGCCT TGCGGCCGAG ACTCAGCCCG TCACAGATGC CAGCCCCATG 
AAGCGCTCCA TCTCCACGCT GGCCCAGCGG CCCCGTGGGA CTCATCTTTG CAGCACCACC 
CCGGACCGCC CACCCCCTAG CCAGGCGTCG TCGCACCACC ACCACCACCG CTGCCACCGC 
CGCAGGGACA GGAAGCAGAG GTCCCTGGAG AAGGGGCCCA GCCTGTCTGC CGATATGGAT 
GGCGCACCAA GCAGTGCTGT GGGGCCGGGG CTGCCCCCGG GAGAGGGGCC TACAGGCTGC 
CGGCGGGAAC GAGAGCGCCG GCAGGAGCGG GGCCGGTCCC AGGAGCGGAG GCAGCCCTCA 
TCCTCCTCCT CGGAGAAGCA GCGCTTCTAC TCCTGCGACC GCTTTGGGGG CCGTGAGCCC 
CCGAAGCCCA AGCCCTCCCT CAGCAGCCAC CCAACGTCGC CAACAGCTGG CCAGGAGCCG 
GGACCCCACC CACAGGGCAG TGGTTCCGTG AATGGGAGCC CCTTGCTGTC AACATCTGGT 
GCTAGCACCC CCGGCCGCGG TGGGCGGAGG CAGCTCCCCC AGACGCCCCT GACTCCCCGC 
CCCAGCATCA CCTACAAGAC GGCCAACTCC TCACCCATCC ACTTCGCCGG GGCTCAGACC 
AGCCTCCCTG CCTTCTCCCC AGGCCGGCTC AGCCGTGGGC TTTCCGAACA CAACGCCCTG 
CTGCAGAGAG ACCCCCTCAG CCAGCCCCTG GCCCCTGGCT CTCGAATTGG CTCTGACCCT 
TACCTGGGGC AGCGTCTGGA CAGTGAGGCC TCTGTCCACG CCCTGCCTGA GGACACTCTC 
ACTTTCGAGG AGGCTGTGGC CACCAACTCG GGCCGCTCCT CCAGGACTTC CTACGTGTCC 
TCCCTGACCT CCCAGTCTCA CCCTCTCCGC CGCGTGCCCA ACGGTTACCA CTGCACCCTG 
GGACTCAGCT CGGGTGGCCG AGCACGGCAC AGCTACCACC ACCCTGACCA AGACCACTGG 
TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_011518994.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517296.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X7, mRNA.

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CloneID	OHu56565
Clone ID Related Accession (Same CDS sequence)	XM_011518994.2
Accession Version	XM_011518994.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGCAGTGCT GAAGAGAGCG 
GCCACCAAGA AGAGCAGAAA TGACCTGATC CACGCAGAGG AGGGAGAGGA CCGGTTTGCA 
GATCTCTGTG CTGTTGGATC CCCCTTCGCC CGCGCCAGCC TCAAGAGCGG GAAGACAGAG 
AGCTCGTCAT ACTTCCGGAG GAAGGAGAAG ATGTTCCGGT TTTTTATCCG GCGCATGGTG 
AAGGCTCAGA GCTTCTACTG GGTGGTGCTG TGCGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ATTACAACCA GCCGCGGCGG CTTACCACGA CCCTGTATTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGA 
AGCTACTTCC GGTCCTCCTT CAACTGCTTC GACTTTGGGG TCATCGTGGG GAGCGTCTTT 
GAAGTGGTCT GGGCGGCCAT CAAGCCGGGA AGCTCCTTTG GGATCAGTGT GCTGCGGGCC 
CTCCGCCTGC TGAGGATCTT CAAAGTCACG AAGTACTGGA GCTCCCTGCG GAACCTGGTG 
GTGTCCCTGC TGAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCTTGCT CTTCCTGTTC 
ATTGTGGTCT TCGCCCTGCT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTCCAGGAT 
GAGACTCCCA CAACCAACTT CGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC 
CTGACGGGAG AGGACTGGAA TGCAGTGATG TATCACGGGA TCGAATCGCA AGGCGGCGTC 
AGCAAAGGCA TGTTCTCGTC CTTTTACTTC ATTGTCCTGA CACTGTTCGG AAACTACACT 
CTGCTGAATG TCTTTCTGGC CATCGCTGTG GACAACCTGG CCAACGCCCA AGAGCTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA 
GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATCGCCGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTACG GCTGCAGAAC 
CTGCGGGCCA GCTGCGAGGC GCTGTACAGC GAGATGGACC CCGAGGAGCG GCTGCGCTTC 
GCCACTACGC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTGGTGGTG 
GAGCTGGGCC GCGACGGCGC GCGGGGGCCC GTGGGAGGCA AAGCCCGACC TGAGGCTGCG 
GAGGCCCCCG AGGGCGTCGA CCCTCCGCGC AGGCACCACC GGCACCGCGA CAAGGACAAG 
ACCCCCGCGG CGGGGGACCA GGACCGAGCA GAGGCCCCGA AGGCGGAGAG CGGGGAGCCC 
GGTGCCCGGG AGGAGCGGCC GCGGCCGCAC CGCAGCCACA GCAAGGAGGC CGCGGGGCCC 
CCGGAGGCGC GGAGCGAGCG CGGCCGAGGC CCAGGCCCCG AGGGCGGCCG GCGGCACCAC 
CGGCGCGGCT CCCCGGAGGA GGCGGCCGAG CGGGAGCCCC GACGCCACCG CGCGCACCGG 
CACCAGGATC CGAGCAAGGA GTGCGCCGGC GCCAAGGGCG AGCGGCGCGC GCGGCACCGC 
GGCGGCCCCC GAGCGGGGCC CCGGGAGGCG GAGAGCGGGG AGGAGCCGGC GCGGCGGCAC 
CGGGCCCGGC ACAAGGCGCA GCCTGCTCAC GAGGCTGTGG AGAAGGAGAC CACGGAGAAG 
GAGGCCACGG AGAAGGAGGC TGAGATAGTG GAAGCCGACA AGGAAAAGGA GCTCCGGAAC 
CACCAGCCCC GGGAGCCACA CTGTGACCTG GAGACCAGTG GGACTGTGAC TGTGGGTCCC 
ATGCACACAC TGCCCAGCAC CTGTCTCCAG AAGGTGGAGG AACAGCCAGA GGATGCAGAC 
AATCAGCGGA ACGTCACTCG CATGGGCAGT CAGCCCCCAG ACCCGAACAC TATTGTACAT 
ATCCCAGTGA TGCTGACGGG CCCTCTTGGG GAAGCCACGG TCGTTCCCAG TGGTAACGTG 
GACCTGGAAA GCCAAGCAGA GGGGAAGAAG GAGGTGGAAG CGGATGACGT GATGAGGAGC 
GGCCCCCGGC CTATCGTCCC ATACAGCTCC ATGTTCTGTT TAAGCCCCAC CAACCTGCTC 
CGCCGCTTCT GCCACTACAT CGTGACCATG AGGTACTTCG AGGTGGTCAT TCTCGTGGTC 
ATCGCCTTGA GCAGCATCGC CCTGGCTGCT GAGGACCCAG TGCGCACAGA CTCGCCCAGG 
AACAACGCTC TGAAATACCT GGATTACATT TTCACTGGTG TCTTTACCTT TGAGATGGTG 
ATAAAGATGA TCGACTTGGG ACTGCTGCTT CACCCTGGAG CCTATTTCCG GGACTTGTGG 
AACATTCTGG ACTTCATTGT GGTCAGTGGC GCCCTGGTGG CGTTTGCTTT CTCAGGATCC 
AAAGGGAAAG ACATCAATAC CATCAAGTCT CTGAGAGTCC TTCGTGTCCT GCGGCCCCTC 
AAGACCATCA AACGGCTGCC CAAGCTCAAG GCTGTGTTTG ACTGTGTGGT GAACTCCCTG 
AAGAATGTCC TCAACATCTT GATTGTCTAC ATGCTCTTCA TGTTCATATT TGCCGTCATT 
GCGGTGCAGC TCTTCAAAGG GAAGTTTTTC TACTGCACAG ATGAATCCAA GGAGCTGGAG 
AGGGACTGCA GGGGTCAGTA TTTGGATTAT GAGAAGGAGG AAGTGGAAGC TCAGCCCAGG 
CAGTGGAAGA AATACGACTT TCACTACGAC AATGTGCTCT GGGCTCTGCT GACGCTGTTC 
ACAGTGTCCA CGGGAGAAGG CTGGCCCATG GTGCTGAAAC ACTCCGTGGA TGCCACCTAT 
GAGGAGCAGG GTCCAAGCCC TGGGTACCGC ATGGAGCTGT CCATCTTCTA CGTGGTCTAC 
TTTGTGGTCT TTCCCTTCTT CTTCGTCAAC ATCTTTGTGG CTTTGATCAT CATCACCTTC 
CAGGAGCAGG GGGACAAGGT GATGTCTGAA TGCAGCCTGG AGAAGAACGA GAGGGCTTGC 
ATTGACTTCG CCATCAGCGC CAAACCCCTG ACACGGTACA TGCCCCAAAA CCGGCAGTCG 
TTCCAGTATA AGACGTGGAC ATTTGTGGTC TCCCCGCCCT TTGAATACTT CATCATGGCC 
ATGATAGCCC TCAACACTGT GGTGCTGATG ATGAAGTTCT ATGATGCACC CTATGAGTAC 
GAGCTGATGC TGAAATGCCT GAACATCGTG TTCACATCCA TGTTCTCCAT GGAATGCGTG 
CTGAAGATCA TCGCCTTTGG GGTGCTGAAC TATTTCAGAG ATGCCTGGAA TGTCTTTGAC 
TTTGTCACTG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GAACAATTTC 
ATCAACCTCA GCTTCCTCCG CCTCTTTCGA GCTGCGCGGC TGATCAAGCT GCTCCGCCAG 
GGCTACACCA TCCGCATCCT GCTGTGGACC TTTGTCCAGT CCTTCAAGGC CCTGCCCTAC 
GTGTGTCTGC TCATTGCCAT GCTGTTCTTC ATCTACGCCA TCATCGGCAT GCAGGTGTTT 
GGGAATATTG CCCTGGATGA TGACACCAGC ATCAACCGCC ACAACAACTT CCGGACGTTT 
TTGCAAGCCC TGATGCTGCT GTTCAGGAGC GCCACGGGGG AGGCCTGGCA CGAGATCATG 
CTGTCCTGCC TGAGCAACCA GGCCTGTGAT GAGCAGGCCA ATGCCACCGA GTGTGGAAGT 
GACTTTGCCT ACTTCTACTT CGTCTCCTTC ATCTTCCTGT GCTCCTTTCT GATGTTGAAC 
CTCTTTGTGG CTGTGATCAT GGACAATTTT GAGTACCTCA CGCGGGACTC TTCCATCCTA 
GGTCCTCACC ACTTGGATGA GTTCATCCGG GTCTGGGCTG AATACGACCC GGCTGCGTGT 
GGGCGCATCA GTTACAATGA CATGTTTGAG ATGCTGAAAC ACATGTCCCC GCCTCTGGGG 
CTGGGGAAGA AATGCCCTGC TCGAGTTGCT TACAAGCGCC TGGTTCGCAT GAACATGCCC 
ATCTCCAACG AGGACATGAC TGTTCACTTC ACGTCCACGC TGATGGCCCT CATCCGGACG 
GCACTGGAGA TCAAGCTGGC CCCAGCTGGG ACAAAGCAGC ATCAGTGTGA CGCGGAGTTG 
AGGAAGGAGA TTTCCGTTGT GTGGGCCAAT CTGCCCCAGA AGACTTTGGA CTTGCTGGTA 
CCACCCCATA AGCCTGATGA GATGACAGTG GGGAAGGTTT ATGCAGCTCT GATGATATTC 
GACTTCTACA AGCAGAACAA AACCACCAGA GACCAGATGC AGCAGGCTCC TGGAGGCCTC 
TCCCAGATGG GTCCTGTGTC CCTGTTCCAC CCTCTGAAGG CCACCCTGGA GCAGACACAG 
CCGGCTGTGC TCCGAGGAGC CCGGGTTTTC CTTCGACAGA AGAGTTCCAC CTCCCTCAGC 
AATGGCGGGG CCATACAAAA CCAAGAGAGT GGCATCAAAG AGTCTGTCTC CTGGGGCACT 
CAAAGGACCC AGGATGCACC CCATGAGGCC AGGCCACCCC TGGAGCGTGG CCACTCCACA 
GAGATCCCTG TGGGGCGGTC AGGAGCACTG GCTGTGGACG TTCAGATGCA GAGCATAACC 
CGGAGGGGCC CTGATGGGGA GCCCCAGCCT GGGCTGGAGA GCCAGGGTCG AGCGGCCTCC 
ATGCCCCGCC TTGCGGCCGA GACTCAGCCC GTCACAGATG CCAGCCCCAT GAAGCGCTCC 
ATCTCCACGC TGGCCCAGCG GCCCCGTGGG ACTCATCTTT GCAGCACCAC CCCGGACCGC 
CCACCCCCTA GCCAGGCGTC GTCGCACCAC CACCACCACC GCTGCCACCG CCGCAGGGAC 
AGGAAGCAGA GGTCCCTGGA GAAGGGGCCC AGCCTGTCTG CCGATATGGA TGGCGCACCA 
AGCAGTGCTG TGGGGCCGGG GCTGCCCCCG GGAGAGGGGC CTACAGGCTG CCGGCGGGAA 
CGAGAGCGCC GGCAGGAGCG GGGCCGGTCC CAGGAGCGGA GGCAGCCCTC ATCCTCCTCC 
TCGGAGAAGC AGCGCTTCTA CTCCTGCGAC CGCTTTGGGG GCCGTGAGCC CCCGAAGCCC 
AAGCCCTCCC TCAGCAGCCA CCCAACGTCG CCAACAGCTG GCCAGGAGCC GGGACCCCAC 
CCACAGGGCA GTGGTTCCGT GAATGGGAGC CCCTTGCTGT CAACATCTGG TGCTAGCACC 
CCCGGCCGCG GTGGGCGGAG GCAGCTCCCC CAGACGCCCC TGACTCCCCG CCCCAGCATC 
ACCTACAAGA CGGCCAACTC CTCACCCATC CACTTCGCCG GGGCTCAGAC CAGCCTCCCT 
GCCTTCTCCC CAGGCCGGCT CAGCCGTGGG CTTTCCGAAC ACAACGCCCT GCTGCAGAGA 
GACCCCCTCA GCCAGCCCCT GGCCCCTGGC TCTCGAATTG GCTCTGACCC TTACCTGGGG 
CAGCGTCTGG ACAGTGAGGC CTCTGTCCAC GCCCTGCCTG AGGACACTCT CACTTTCGAG 
GAGGCTGTGG CCACCAACTC GGGCCGCTCC TCCAGGACTT CCTACGTGTC CTCCCTGACC 
TCCCAGTCTC ACCCTCTCCG CCGCGTGCCC AACGGTTACC ACTGCACCCT GGGACTCAGC 
TCGGGTGGCC GAGCACGGCA CAGCTACCAC CACCCTGACC AAGACCACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517296.1
CDS	109..7125
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPIHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPGSRIGSDPYLGQRLDSEASVHALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_011518994.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518994.2

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGCAGTGCT GAAGAGAGCG 
GCCACCAAGA AGAGCAGAAA TGACCTGATC CACGCAGAGG AGGGAGAGGA CCGGTTTGCA 
GATCTCTGTG CTGTTGGATC CCCCTTCGCC CGCGCCAGCC TCAAGAGCGG GAAGACAGAG 
AGCTCGTCAT ACTTCCGGAG GAAGGAGAAG ATGTTCCGGT TTTTTATCCG GCGCATGGTG 
AAGGCTCAGA GCTTCTACTG GGTGGTGCTG TGCGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ATTACAACCA GCCGCGGCGG CTTACCACGA CCCTGTATTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGA 
AGCTACTTCC GGTCCTCCTT CAACTGCTTC GACTTTGGGG TCATCGTGGG GAGCGTCTTT 
GAAGTGGTCT GGGCGGCCAT CAAGCCGGGA AGCTCCTTTG GGATCAGTGT GCTGCGGGCC 
CTCCGCCTGC TGAGGATCTT CAAAGTCACG AAGTACTGGA GCTCCCTGCG GAACCTGGTG 
GTGTCCCTGC TGAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCTTGCT CTTCCTGTTC 
ATTGTGGTCT TCGCCCTGCT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTCCAGGAT 
GAGACTCCCA CAACCAACTT CGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC 
CTGACGGGAG AGGACTGGAA TGCAGTGATG TATCACGGGA TCGAATCGCA AGGCGGCGTC 
AGCAAAGGCA TGTTCTCGTC CTTTTACTTC ATTGTCCTGA CACTGTTCGG AAACTACACT 
CTGCTGAATG TCTTTCTGGC CATCGCTGTG GACAACCTGG CCAACGCCCA AGAGCTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA 
GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATCGCCGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTACG GCTGCAGAAC 
CTGCGGGCCA GCTGCGAGGC GCTGTACAGC GAGATGGACC CCGAGGAGCG GCTGCGCTTC 
GCCACTACGC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTGGTGGTG 
GAGCTGGGCC GCGACGGCGC GCGGGGGCCC GTGGGAGGCA AAGCCCGACC TGAGGCTGCG 
GAGGCCCCCG AGGGCGTCGA CCCTCCGCGC AGGCACCACC GGCACCGCGA CAAGGACAAG 
ACCCCCGCGG CGGGGGACCA GGACCGAGCA GAGGCCCCGA AGGCGGAGAG CGGGGAGCCC 
GGTGCCCGGG AGGAGCGGCC GCGGCCGCAC CGCAGCCACA GCAAGGAGGC CGCGGGGCCC 
CCGGAGGCGC GGAGCGAGCG CGGCCGAGGC CCAGGCCCCG AGGGCGGCCG GCGGCACCAC 
CGGCGCGGCT CCCCGGAGGA GGCGGCCGAG CGGGAGCCCC GACGCCACCG CGCGCACCGG 
CACCAGGATC CGAGCAAGGA GTGCGCCGGC GCCAAGGGCG AGCGGCGCGC GCGGCACCGC 
GGCGGCCCCC GAGCGGGGCC CCGGGAGGCG GAGAGCGGGG AGGAGCCGGC GCGGCGGCAC 
CGGGCCCGGC ACAAGGCGCA GCCTGCTCAC GAGGCTGTGG AGAAGGAGAC CACGGAGAAG 
GAGGCCACGG AGAAGGAGGC TGAGATAGTG GAAGCCGACA AGGAAAAGGA GCTCCGGAAC 
CACCAGCCCC GGGAGCCACA CTGTGACCTG GAGACCAGTG GGACTGTGAC TGTGGGTCCC 
ATGCACACAC TGCCCAGCAC CTGTCTCCAG AAGGTGGAGG AACAGCCAGA GGATGCAGAC 
AATCAGCGGA ACGTCACTCG CATGGGCAGT CAGCCCCCAG ACCCGAACAC TATTGTACAT 
ATCCCAGTGA TGCTGACGGG CCCTCTTGGG GAAGCCACGG TCGTTCCCAG TGGTAACGTG 
GACCTGGAAA GCCAAGCAGA GGGGAAGAAG GAGGTGGAAG CGGATGACGT GATGAGGAGC 
GGCCCCCGGC CTATCGTCCC ATACAGCTCC ATGTTCTGTT TAAGCCCCAC CAACCTGCTC 
CGCCGCTTCT GCCACTACAT CGTGACCATG AGGTACTTCG AGGTGGTCAT TCTCGTGGTC 
ATCGCCTTGA GCAGCATCGC CCTGGCTGCT GAGGACCCAG TGCGCACAGA CTCGCCCAGG 
AACAACGCTC TGAAATACCT GGATTACATT TTCACTGGTG TCTTTACCTT TGAGATGGTG 
ATAAAGATGA TCGACTTGGG ACTGCTGCTT CACCCTGGAG CCTATTTCCG GGACTTGTGG 
AACATTCTGG ACTTCATTGT GGTCAGTGGC GCCCTGGTGG CGTTTGCTTT CTCAGGATCC 
AAAGGGAAAG ACATCAATAC CATCAAGTCT CTGAGAGTCC TTCGTGTCCT GCGGCCCCTC 
AAGACCATCA AACGGCTGCC CAAGCTCAAG GCTGTGTTTG ACTGTGTGGT GAACTCCCTG 
AAGAATGTCC TCAACATCTT GATTGTCTAC ATGCTCTTCA TGTTCATATT TGCCGTCATT 
GCGGTGCAGC TCTTCAAAGG GAAGTTTTTC TACTGCACAG ATGAATCCAA GGAGCTGGAG 
AGGGACTGCA GGGGTCAGTA TTTGGATTAT GAGAAGGAGG AAGTGGAAGC TCAGCCCAGG 
CAGTGGAAGA AATACGACTT TCACTACGAC AATGTGCTCT GGGCTCTGCT GACGCTGTTC 
ACAGTGTCCA CGGGAGAAGG CTGGCCCATG GTGCTGAAAC ACTCCGTGGA TGCCACCTAT 
GAGGAGCAGG GTCCAAGCCC TGGGTACCGC ATGGAGCTGT CCATCTTCTA CGTGGTCTAC 
TTTGTGGTCT TTCCCTTCTT CTTCGTCAAC ATCTTTGTGG CTTTGATCAT CATCACCTTC 
CAGGAGCAGG GGGACAAGGT GATGTCTGAA TGCAGCCTGG AGAAGAACGA GAGGGCTTGC 
ATTGACTTCG CCATCAGCGC CAAACCCCTG ACACGGTACA TGCCCCAAAA CCGGCAGTCG 
TTCCAGTATA AGACGTGGAC ATTTGTGGTC TCCCCGCCCT TTGAATACTT CATCATGGCC 
ATGATAGCCC TCAACACTGT GGTGCTGATG ATGAAGTTCT ATGATGCACC CTATGAGTAC 
GAGCTGATGC TGAAATGCCT GAACATCGTG TTCACATCCA TGTTCTCCAT GGAATGCGTG 
CTGAAGATCA TCGCCTTTGG GGTGCTGAAC TATTTCAGAG ATGCCTGGAA TGTCTTTGAC 
TTTGTCACTG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GAACAATTTC 
ATCAACCTCA GCTTCCTCCG CCTCTTTCGA GCTGCGCGGC TGATCAAGCT GCTCCGCCAG 
GGCTACACCA TCCGCATCCT GCTGTGGACC TTTGTCCAGT CCTTCAAGGC CCTGCCCTAC 
GTGTGTCTGC TCATTGCCAT GCTGTTCTTC ATCTACGCCA TCATCGGCAT GCAGGTGTTT 
GGGAATATTG CCCTGGATGA TGACACCAGC ATCAACCGCC ACAACAACTT CCGGACGTTT 
TTGCAAGCCC TGATGCTGCT GTTCAGGAGC GCCACGGGGG AGGCCTGGCA CGAGATCATG 
CTGTCCTGCC TGAGCAACCA GGCCTGTGAT GAGCAGGCCA ATGCCACCGA GTGTGGAAGT 
GACTTTGCCT ACTTCTACTT CGTCTCCTTC ATCTTCCTGT GCTCCTTTCT GATGTTGAAC 
CTCTTTGTGG CTGTGATCAT GGACAATTTT GAGTACCTCA CGCGGGACTC TTCCATCCTA 
GGTCCTCACC ACTTGGATGA GTTCATCCGG GTCTGGGCTG AATACGACCC GGCTGCGTGT 
GGGCGCATCA GTTACAATGA CATGTTTGAG ATGCTGAAAC ACATGTCCCC GCCTCTGGGG 
CTGGGGAAGA AATGCCCTGC TCGAGTTGCT TACAAGCGCC TGGTTCGCAT GAACATGCCC 
ATCTCCAACG AGGACATGAC TGTTCACTTC ACGTCCACGC TGATGGCCCT CATCCGGACG 
GCACTGGAGA TCAAGCTGGC CCCAGCTGGG ACAAAGCAGC ATCAGTGTGA CGCGGAGTTG 
AGGAAGGAGA TTTCCGTTGT GTGGGCCAAT CTGCCCCAGA AGACTTTGGA CTTGCTGGTA 
CCACCCCATA AGCCTGATGA GATGACAGTG GGGAAGGTTT ATGCAGCTCT GATGATATTC 
GACTTCTACA AGCAGAACAA AACCACCAGA GACCAGATGC AGCAGGCTCC TGGAGGCCTC 
TCCCAGATGG GTCCTGTGTC CCTGTTCCAC CCTCTGAAGG CCACCCTGGA GCAGACACAG 
CCGGCTGTGC TCCGAGGAGC CCGGGTTTTC CTTCGACAGA AGAGTTCCAC CTCCCTCAGC 
AATGGCGGGG CCATACAAAA CCAAGAGAGT GGCATCAAAG AGTCTGTCTC CTGGGGCACT 
CAAAGGACCC AGGATGCACC CCATGAGGCC AGGCCACCCC TGGAGCGTGG CCACTCCACA 
GAGATCCCTG TGGGGCGGTC AGGAGCACTG GCTGTGGACG TTCAGATGCA GAGCATAACC 
CGGAGGGGCC CTGATGGGGA GCCCCAGCCT GGGCTGGAGA GCCAGGGTCG AGCGGCCTCC 
ATGCCCCGCC TTGCGGCCGA GACTCAGCCC GTCACAGATG CCAGCCCCAT GAAGCGCTCC 
ATCTCCACGC TGGCCCAGCG GCCCCGTGGG ACTCATCTTT GCAGCACCAC CCCGGACCGC 
CCACCCCCTA GCCAGGCGTC GTCGCACCAC CACCACCACC GCTGCCACCG CCGCAGGGAC 
AGGAAGCAGA GGTCCCTGGA GAAGGGGCCC AGCCTGTCTG CCGATATGGA TGGCGCACCA 
AGCAGTGCTG TGGGGCCGGG GCTGCCCCCG GGAGAGGGGC CTACAGGCTG CCGGCGGGAA 
CGAGAGCGCC GGCAGGAGCG GGGCCGGTCC CAGGAGCGGA GGCAGCCCTC ATCCTCCTCC 
TCGGAGAAGC AGCGCTTCTA CTCCTGCGAC CGCTTTGGGG GCCGTGAGCC CCCGAAGCCC 
AAGCCCTCCC TCAGCAGCCA CCCAACGTCG CCAACAGCTG GCCAGGAGCC GGGACCCCAC 
CCACAGGGCA GTGGTTCCGT GAATGGGAGC CCCTTGCTGT CAACATCTGG TGCTAGCACC 
CCCGGCCGCG GTGGGCGGAG GCAGCTCCCC CAGACGCCCC TGACTCCCCG CCCCAGCATC 
ACCTACAAGA CGGCCAACTC CTCACCCATC CACTTCGCCG GGGCTCAGAC CAGCCTCCCT 
GCCTTCTCCC CAGGCCGGCT CAGCCGTGGG CTTTCCGAAC ACAACGCCCT GCTGCAGAGA 
GACCCCCTCA GCCAGCCCCT GGCCCCTGGC TCTCGAATTG GCTCTGACCC TTACCTGGGG 
CAGCGTCTGG ACAGTGAGGC CTCTGTCCAC GCCCTGCCTG AGGACACTCT CACTTTCGAG 
GAGGCTGTGG CCACCAACTC GGGCCGCTCC TCCAGGACTT CCTACGTGTC CTCCCTGACC 
TCCCAGTCTC ACCCTCTCCG CCGCGTGCCC AACGGTTACC ACTGCACCCT GGGACTCAGC 
TCGGGTGGCC GAGCACGGCA CAGCTACCAC CACCCTGACC AAGACCACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_017015115.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870604.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X2, mRNA.

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CloneID	OHu98898
Clone ID Related Accession (Same CDS sequence)	XM_017015115.1
Accession Version	XM_017015115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC GAGCTTCGTG 
GGAGGATCCA AAGGGAAAGA CATCAATACC ATCAAGTCTC TGAGAGTCCT TCGTGTCCTG 
CGGCCCCTCA AGACCATCAA ACGGCTGCCC AAGCTCAAGG CTGTGTTTGA CTGTGTGGTG 
AACTCCCTGA AGAATGTCCT CAACATCTTG ATTGTCTACA TGCTCTTCAT GTTCATATTT 
GCCGTCATTG CGGTGCAGCT CTTCAAAGGG AAGTTTTTCT ACTGCACAGA TGAATCCAAG 
GAGCTGGAGA GGGACTGCAG GGGTCAGTAT TTGGATTATG AGAAGGAGGA AGTGGAAGCT 
CAGCCCAGGC AGTGGAAGAA ATACGACTTT CACTACGACA ATGTGCTCTG GGCTCTGCTG 
ACGCTGTTCA CAGTGTCCAC GGGAGAAGGC TGGCCCATGG TGCTGAAACA CTCCGTGGAT 
GCCACCTATG AGGAGCAGGG TCCAAGCCCT GGGTACCGCA TGGAGCTGTC CATCTTCTAC 
GTGGTCTACT TTGTGGTCTT TCCCTTCTTC TTCGTCAACA TCTTTGTGGC TTTGATCATC 
ATCACCTTCC AGGAGCAGGG GGACAAGGTG ATGTCTGAAT GCAGCCTGGA GAAGAACGAG 
AGGGCTTGCA TTGACTTCGC CATCAGCGCC AAACCCCTGA CACGGTACAT GCCCCAAAAC 
CGGCAGTCGT TCCAGTATAA GACGTGGACA TTTGTGGTCT CCCCGCCCTT TGAATACTTC 
ATCATGGCCA TGATAGCCCT CAACACTGTG GTGCTGATGA TGAAGTTCTA TGATGCACCC 
TATGAGTACG AGCTGATGCT GAAATGCCTG AACATCGTGT TCACATCCAT GTTCTCCATG 
GAATGCGTGC TGAAGATCAT CGCCTTTGGG GTGCTGAACT ATTTCAGAGA TGCCTGGAAT 
GTCTTTGACT TTGTCACTGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GAAACGAACA ATTTCATCAA CCTCAGCTTC CTCCGCCTCT TTCGAGCTGC GCGGCTGATC 
AAGCTGCTCC GCCAGGGCTA CACCATCCGC ATCCTGCTGT GGACCTTTGT CCAGTCCTTC 
AAGGCCCTGC CCTACGTGTG TCTGCTCATT GCCATGCTGT TCTTCATCTA CGCCATCATC 
GGCATGCAGG TGTTTGGGAA TATTGCCCTG GATGATGACA CCAGCATCAA CCGCCACAAC 
AACTTCCGGA CGTTTTTGCA AGCCCTGATG CTGCTGTTCA GGAGCGCCAC GGGGGAGGCC 
TGGCACGAGA TCATGCTGTC CTGCCTGAGC AACCAGGCCT GTGATGAGCA GGCCAATGCC 
ACCGAGTGTG GAAGTGACTT TGCCTACTTC TACTTCGTCT CCTTCATCTT CCTGTGCTCC 
TTTCTGATGT TGAACCTCTT TGTGGCTGTG ATCATGGACA ATTTTGAGTA CCTCACGCGG 
GACTCTTCCA TCCTAGGTCC TCACCACTTG GATGAGTTCA TCCGGGTCTG GGCTGAATAC 
GACCCGGCTG CGTGTGGGCG CATCAGTTAC AATGACATGT TTGAGATGCT GAAACACATG 
TCCCCGCCTC TGGGGCTGGG GAAGAAATGC CCTGCTCGAG TTGCTTACAA GCGCCTGGTT 
CGCATGAACA TGCCCATCTC CAACGAGGAC ATGACTGTTC ACTTCACGTC CACGCTGATG 
GCCCTCATCC GGACGGCACT GGAGATCAAG CTGGCCCCAG CTGGGACAAA GCAGCATCAG 
TGTGACGCGG AGTTGAGGAA GGAGATTTCC GTTGTGTGGG CCAATCTGCC CCAGAAGACT 
TTGGACTTGC TGGTACCACC CCATAAGCCT GATGAGATGA CAGTGGGGAA GGTTTATGCA 
GCTCTGATGA TATTCGACTT CTACAAGCAG AACAAAACCA CCAGAGACCA GATGCAGCAG 
GCTCCTGGAG GCCTCTCCCA GATGGGTCCT GTGTCCCTGT TCCACCCTCT GAAGGCCACC 
CTGGAGCAGA CACAGCCGGC TGTGCTCCGA GGAGCCCGGG TTTTCCTTCG ACAGAAGAGT 
TCCACCTCCC TCAGCAATGG CGGGGCCATA CAAAACCAAG AGAGTGGCAT CAAAGAGTCT 
GTCTCCTGGG GCACTCAAAG GACCCAGGAT GCACCCCATG AGGCCAGGCC ACCCCTGGAG 
CGTGGCCACT CCACAGAGAT CCCTGTGGGG CGGTCAGGAG CACTGGCTGT GGACGTTCAG 
ATGCAGAGCA TAACCCGGAG GGGCCCTGAT GGGGAGCCCC AGCCTGGGCT GGAGAGCCAG 
GGTCGAGCGG CCTCCATGCC CCGCCTTGCG GCCGAGACTC AGCCCGTCAC AGATGCCAGC 
CCCATGAAGC GCTCCATCTC CACGCTGGCC CAGCGGCCCC GTGGGACTCA TCTTTGCAGC 
ACCACCCCGG ACCGCCCACC CCCTAGCCAG GCGTCGTCGC ACCACCACCA CCACCGCTGC 
CACCGCCGCA GGGACAGGAA GCAGAGGTCC CTGGAGAAGG GGCCCAGCCT GTCTGCCGAT 
ATGGATGGCG CACCAAGCAG TGCTGTGGGG CCGGGGCTGC CCCCGGGAGA GGGGCCTACA 
GGCTGCCGGC GGGAACGAGA GCGCCGGCAG GAGCGGGGCC GGTCCCAGGA GCGGAGGCAG 
CCCTCATCCT CCTCCTCGGA GAAGCAGCGC TTCTACTCCT GCGACCGCTT TGGGGGCCGT 
GAGCCCCCGA AGCCCAAGCC CTCCCTCAGC AGCCACCCAA CGTCGCCAAC AGCTGGCCAG 
GAGCCGGGAC CCCACCCACA GGGCAGTGGT TCCGTGAATG GGAGCCCCTT GCTGTCAACA 
TCTGGTGCTA GCACCCCCGG CCGCGGTGGG CGGAGGCAGC TCCCCCAGAC GCCCCTGACT 
CCCCGCCCCA GCATCACCTA CAAGACGGCC AACTCCTCAC CCATCCACTT CGCCGGGGCT 
CAGACCAGCC TCCCTGCCTT CTCCCCAGGC CGGCTCAGCC GTGGGCTTTC CGAACACAAC 
GCCCTGCTGC AGAGAGACCC CCTCAGCCAG CCCCTGGCCC CTGGCTCTCG AATTGGCTCT 
GACCCTTACC TGGGGCAGCG TCTGGACAGT GAGGCCTCTG TCCACGCCCT GCCTGAGGAC 
ACTCTCACTT TCGAGGAGGC TGTGGCCACC AACTCGGGCC GCTCCTCCAG GACTTCCTAC 
GTGTCCTCCC TGACCTCCCA GTCTCACCCT CTCCGCCGCG TGCCCAACGG TTACCACTGC 
ACCCTGGGAC TCAGCTCGGG TGGCCGAGCA CGGCACAGCT ACCACCACCC TGACCAAGAC 
CACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870604.1
CDS	110..7141
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFVGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPIHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPGSRIGSDPYLGQRLDSEASVHALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_017015115.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015115.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC GAGCTTCGTG 
GGAGGATCCA AAGGGAAAGA CATCAATACC ATCAAGTCTC TGAGAGTCCT TCGTGTCCTG 
CGGCCCCTCA AGACCATCAA ACGGCTGCCC AAGCTCAAGG CTGTGTTTGA CTGTGTGGTG 
AACTCCCTGA AGAATGTCCT CAACATCTTG ATTGTCTACA TGCTCTTCAT GTTCATATTT 
GCCGTCATTG CGGTGCAGCT CTTCAAAGGG AAGTTTTTCT ACTGCACAGA TGAATCCAAG 
GAGCTGGAGA GGGACTGCAG GGGTCAGTAT TTGGATTATG AGAAGGAGGA AGTGGAAGCT 
CAGCCCAGGC AGTGGAAGAA ATACGACTTT CACTACGACA ATGTGCTCTG GGCTCTGCTG 
ACGCTGTTCA CAGTGTCCAC GGGAGAAGGC TGGCCCATGG TGCTGAAACA CTCCGTGGAT 
GCCACCTATG AGGAGCAGGG TCCAAGCCCT GGGTACCGCA TGGAGCTGTC CATCTTCTAC 
GTGGTCTACT TTGTGGTCTT TCCCTTCTTC TTCGTCAACA TCTTTGTGGC TTTGATCATC 
ATCACCTTCC AGGAGCAGGG GGACAAGGTG ATGTCTGAAT GCAGCCTGGA GAAGAACGAG 
AGGGCTTGCA TTGACTTCGC CATCAGCGCC AAACCCCTGA CACGGTACAT GCCCCAAAAC 
CGGCAGTCGT TCCAGTATAA GACGTGGACA TTTGTGGTCT CCCCGCCCTT TGAATACTTC 
ATCATGGCCA TGATAGCCCT CAACACTGTG GTGCTGATGA TGAAGTTCTA TGATGCACCC 
TATGAGTACG AGCTGATGCT GAAATGCCTG AACATCGTGT TCACATCCAT GTTCTCCATG 
GAATGCGTGC TGAAGATCAT CGCCTTTGGG GTGCTGAACT ATTTCAGAGA TGCCTGGAAT 
GTCTTTGACT TTGTCACTGT GTTGGGAAGT ATTACTGATA TTTTAGTAAC AGAGATTGCG 
GAAACGAACA ATTTCATCAA CCTCAGCTTC CTCCGCCTCT TTCGAGCTGC GCGGCTGATC 
AAGCTGCTCC GCCAGGGCTA CACCATCCGC ATCCTGCTGT GGACCTTTGT CCAGTCCTTC 
AAGGCCCTGC CCTACGTGTG TCTGCTCATT GCCATGCTGT TCTTCATCTA CGCCATCATC 
GGCATGCAGG TGTTTGGGAA TATTGCCCTG GATGATGACA CCAGCATCAA CCGCCACAAC 
AACTTCCGGA CGTTTTTGCA AGCCCTGATG CTGCTGTTCA GGAGCGCCAC GGGGGAGGCC 
TGGCACGAGA TCATGCTGTC CTGCCTGAGC AACCAGGCCT GTGATGAGCA GGCCAATGCC 
ACCGAGTGTG GAAGTGACTT TGCCTACTTC TACTTCGTCT CCTTCATCTT CCTGTGCTCC 
TTTCTGATGT TGAACCTCTT TGTGGCTGTG ATCATGGACA ATTTTGAGTA CCTCACGCGG 
GACTCTTCCA TCCTAGGTCC TCACCACTTG GATGAGTTCA TCCGGGTCTG GGCTGAATAC 
GACCCGGCTG CGTGTGGGCG CATCAGTTAC AATGACATGT TTGAGATGCT GAAACACATG 
TCCCCGCCTC TGGGGCTGGG GAAGAAATGC CCTGCTCGAG TTGCTTACAA GCGCCTGGTT 
CGCATGAACA TGCCCATCTC CAACGAGGAC ATGACTGTTC ACTTCACGTC CACGCTGATG 
GCCCTCATCC GGACGGCACT GGAGATCAAG CTGGCCCCAG CTGGGACAAA GCAGCATCAG 
TGTGACGCGG AGTTGAGGAA GGAGATTTCC GTTGTGTGGG CCAATCTGCC CCAGAAGACT 
TTGGACTTGC TGGTACCACC CCATAAGCCT GATGAGATGA CAGTGGGGAA GGTTTATGCA 
GCTCTGATGA TATTCGACTT CTACAAGCAG AACAAAACCA CCAGAGACCA GATGCAGCAG 
GCTCCTGGAG GCCTCTCCCA GATGGGTCCT GTGTCCCTGT TCCACCCTCT GAAGGCCACC 
CTGGAGCAGA CACAGCCGGC TGTGCTCCGA GGAGCCCGGG TTTTCCTTCG ACAGAAGAGT 
TCCACCTCCC TCAGCAATGG CGGGGCCATA CAAAACCAAG AGAGTGGCAT CAAAGAGTCT 
GTCTCCTGGG GCACTCAAAG GACCCAGGAT GCACCCCATG AGGCCAGGCC ACCCCTGGAG 
CGTGGCCACT CCACAGAGAT CCCTGTGGGG CGGTCAGGAG CACTGGCTGT GGACGTTCAG 
ATGCAGAGCA TAACCCGGAG GGGCCCTGAT GGGGAGCCCC AGCCTGGGCT GGAGAGCCAG 
GGTCGAGCGG CCTCCATGCC CCGCCTTGCG GCCGAGACTC AGCCCGTCAC AGATGCCAGC 
CCCATGAAGC GCTCCATCTC CACGCTGGCC CAGCGGCCCC GTGGGACTCA TCTTTGCAGC 
ACCACCCCGG ACCGCCCACC CCCTAGCCAG GCGTCGTCGC ACCACCACCA CCACCGCTGC 
CACCGCCGCA GGGACAGGAA GCAGAGGTCC CTGGAGAAGG GGCCCAGCCT GTCTGCCGAT 
ATGGATGGCG CACCAAGCAG TGCTGTGGGG CCGGGGCTGC CCCCGGGAGA GGGGCCTACA 
GGCTGCCGGC GGGAACGAGA GCGCCGGCAG GAGCGGGGCC GGTCCCAGGA GCGGAGGCAG 
CCCTCATCCT CCTCCTCGGA GAAGCAGCGC TTCTACTCCT GCGACCGCTT TGGGGGCCGT 
GAGCCCCCGA AGCCCAAGCC CTCCCTCAGC AGCCACCCAA CGTCGCCAAC AGCTGGCCAG 
GAGCCGGGAC CCCACCCACA GGGCAGTGGT TCCGTGAATG GGAGCCCCTT GCTGTCAACA 
TCTGGTGCTA GCACCCCCGG CCGCGGTGGG CGGAGGCAGC TCCCCCAGAC GCCCCTGACT 
CCCCGCCCCA GCATCACCTA CAAGACGGCC AACTCCTCAC CCATCCACTT CGCCGGGGCT 
CAGACCAGCC TCCCTGCCTT CTCCCCAGGC CGGCTCAGCC GTGGGCTTTC CGAACACAAC 
GCCCTGCTGC AGAGAGACCC CCTCAGCCAG CCCCTGGCCC CTGGCTCTCG AATTGGCTCT 
GACCCTTACC TGGGGCAGCG TCTGGACAGT GAGGCCTCTG TCCACGCCCT GCCTGAGGAC 
ACTCTCACTT TCGAGGAGGC TGTGGCCACC AACTCGGGCC GCTCCTCCAG GACTTCCTAC 
GTGTCCTCCC TGACCTCCCA GTCTCACCCT CTCCGCCGCG TGCCCAACGG TTACCACTGC 
ACCCTGGGAC TCAGCTCGGG TGGCCGAGCA CGGCACAGCT ACCACCACCC TGACCAAGAC 
CACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_017015116.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870605.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X4, mRNA.

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CloneID	OHu98899
Clone ID Related Accession (Same CDS sequence)	XM_017015116.1
Accession Version	XM_017015116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGCAGTGCT GAAGAGAGCG 
GCCACCAAGA AGAGCAGAAA TGACCTGATC CACGCAGAGG AGGGAGAGGA CCGGTTTGCA 
GATCTCTGTG CTGTTGGATC CCCCTTCGCC CGCGCCAGCC TCAAGAGCGG GAAGACAGAG 
AGCTCGTCAT ACTTCCGGAG GAAGGAGAAG ATGTTCCGGT TTTTTATCCG GCGCATGGTG 
AAGGCTCAGA GCTTCTACTG GGTGGTGCTG TGCGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ATTACAACCA GCCGCGGCGG CTTACCACGA CCCTGTATTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGA 
AGCTACTTCC GGTCCTCCTT CAACTGCTTC GACTTTGGGG TCATCGTGGG GAGCGTCTTT 
GAAGTGGTCT GGGCGGCCAT CAAGCCGGGA AGCTCCTTTG GGATCAGTGT GCTGCGGGCC 
CTCCGCCTGC TGAGGATCTT CAAAGTCACG AAGTACTGGA GCTCCCTGCG GAACCTGGTG 
GTGTCCCTGC TGAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCTTGCT CTTCCTGTTC 
ATTGTGGTCT TCGCCCTGCT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTCCAGGAT 
GAGACTCCCA CAACCAACTT CGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC 
CTGACGGGAG AGGACTGGAA TGCAGTGATG TATCACGGGA TCGAATCGCA AGGCGGCGTC 
AGCAAAGGCA TGTTCTCGTC CTTTTACTTC ATTGTCCTGA CACTGTTCGG AAACTACACT 
CTGCTGAATG TCTTTCTGGC CATCGCTGTG GACAACCTGG CCAACGCCCA AGAGCTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA 
GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATCGCCGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTACG GCTGCAGAAC 
CTGCGGGCCA GCTGCGAGGC GCTGTACAGC GAGATGGACC CCGAGGAGCG GCTGCGCTTC 
GCCACTACGC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTGGTGGTG 
GAGCTGGGCC GCGACGGCGC GCGGGGGCCC GTGGGAGGCA AAGCCCGACC TGAGGCTGCG 
GAGGCCCCCG AGGGCGTCGA CCCTCCGCGC AGGCACCACC GGCACCGCGA CAAGGACAAG 
ACCCCCGCGG CGGGGGACCA GGACCGAGCA GAGGCCCCGA AGGCGGAGAG CGGGGAGCCC 
GGTGCCCGGG AGGAGCGGCC GCGGCCGCAC CGCAGCCACA GCAAGGAGGC CGCGGGGCCC 
CCGGAGGCGC GGAGCGAGCG CGGCCGAGGC CCAGGCCCCG AGGGCGGCCG GCGGCACCAC 
CGGCGCGGCT CCCCGGAGGA GGCGGCCGAG CGGGAGCCCC GACGCCACCG CGCGCACCGG 
CACCAGGATC CGAGCAAGGA GTGCGCCGGC GCCAAGGGCG AGCGGCGCGC GCGGCACCGC 
GGCGGCCCCC GAGCGGGGCC CCGGGAGGCG GAGAGCGGGG AGGAGCCGGC GCGGCGGCAC 
CGGGCCCGGC ACAAGGCGCA GCCTGCTCAC GAGGCTGTGG AGAAGGAGAC CACGGAGAAG 
GAGGCCACGG AGAAGGAGGC TGAGATAGTG GAAGCCGACA AGGAAAAGGA GCTCCGGAAC 
CACCAGCCCC GGGAGCCACA CTGTGACCTG GAGACCAGTG GGACTGTGAC TGTGGGTCCC 
ATGCACACAC TGCCCAGCAC CTGTCTCCAG AAGGTGGAGG AACAGCCAGA GGATGCAGAC 
AATCAGCGGA ACGTCACTCG CATGGGCAGT CAGCCCCCAG ACCCGAACAC TATTGTACAT 
ATCCCAGTGA TGCTGACGGG CCCTCTTGGG GAAGCCACGG TCGTTCCCAG TGGTAACGTG 
GACCTGGAAA GCCAAGCAGA GGGGAAGAAG GAGGTGGAAG CGGATGACGT GATGAGGAGC 
GGCCCCCGGC CTATCGTCCC ATACAGCTCC ATGTTCTGTT TAAGCCCCAC CAACCTGCTC 
CGCCGCTTCT GCCACTACAT CGTGACCATG AGGTACTTCG AGGTGGTCAT TCTCGTGGTC 
ATCGCCTTGA GCAGCATCGC CCTGGCTGCT GAGGACCCAG TGCGCACAGA CTCGCCCAGG 
AACAACGCTC TGAAATACCT GGATTACATT TTCACTGGTG TCTTTACCTT TGAGATGGTG 
ATAAAGATGA TCGACTTGGG ACTGCTGCTT CACCCTGGAG CCTATTTCCG GGACTTGTGG 
AACATTCTGG ACTTCATTGT GGTCAGTGGC GCCCTGGTGG CGTTTGCTTT CTCGAGCTTC 
GTGGGAGGAT CCAAAGGGAA AGACATCAAT ACCATCAAGT CTCTGAGAGT CCTTCGTGTC 
CTGCGGCCCC TCAAGACCAT CAAACGGCTG CCCAAGCTCA AGGCTGTGTT TGACTGTGTG 
GTGAACTCCC TGAAGAATGT CCTCAACATC TTGATTGTCT ACATGCTCTT CATGTTCATA 
TTTGCCGTCA TTGCGGTGCA GCTCTTCAAA GGGAAGTTTT TCTACTGCAC AGATGAATCC 
AAGGAGCTGG AGAGGGACTG CAGGGGTCAG TATTTGGATT ATGAGAAGGA GGAAGTGGAA 
GCTCAGCCCA GGCAGTGGAA GAAATACGAC TTTCACTACG ACAATGTGCT CTGGGCTCTG 
CTGACGCTGT TCACAGTGTC CACGGGAGAA GGCTGGCCCA TGGTGCTGAA ACACTCCGTG 
GATGCCACCT ATGAGGAGCA GGGTCCAAGC CCTGGGTACC GCATGGAGCT GTCCATCTTC 
TACGTGGTCT ACTTTGTGGT CTTTCCCTTC TTCTTCGTCA ACATCTTTGT GGCTTTGATC 
ATCATCACCT TCCAGGAGCA GGGGGACAAG GTGATGTCTG AATGCAGCCT GGAGAAGAAC 
GAGAGGGCTT GCATTGACTT CGCCATCAGC GCCAAACCCC TGACACGGTA CATGCCCCAA 
AACCGGCAGT CGTTCCAGTA TAAGACGTGG ACATTTGTGG TCTCCCCGCC CTTTGAATAC 
TTCATCATGG CCATGATAGC CCTCAACACT GTGGTGCTGA TGATGAAGTT CTATGATGCA 
CCCTATGAGT ACGAGCTGAT GCTGAAATGC CTGAACATCG TGTTCACATC CATGTTCTCC 
ATGGAATGCG TGCTGAAGAT CATCGCCTTT GGGGTGCTGA ACTATTTCAG AGATGCCTGG 
AATGTCTTTG ACTTTGTCAC TGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGAACAATT TCATCAACCT CAGCTTCCTC CGCCTCTTTC GAGCTGCGCG GCTGATCAAG 
CTGCTCCGCC AGGGCTACAC CATCCGCATC CTGCTGTGGA CCTTTGTCCA GTCCTTCAAG 
GCCCTGCCCT ACGTGTGTCT GCTCATTGCC ATGCTGTTCT TCATCTACGC CATCATCGGC 
ATGCAGGTGT TTGGGAATAT TGCCCTGGAT GATGACACCA GCATCAACCG CCACAACAAC 
TTCCGGACGT TTTTGCAAGC CCTGATGCTG CTGTTCAGGA GCGCCACGGG GGAGGCCTGG 
CACGAGATCA TGCTGTCCTG CCTGAGCAAC CAGGCCTGTG ATGAGCAGGC CAATGCCACC 
GAGTGTGGAA GTGACTTTGC CTACTTCTAC TTCGTCTCCT TCATCTTCCT GTGCTCCTTT 
CTGATGTTGA ACCTCTTTGT GGCTGTGATC ATGGACAATT TTGAGTACCT CACGCGGGAC 
TCTTCCATCC TAGGTCCTCA CCACTTGGAT GAGTTCATCC GGGTCTGGGC TGAATACGAC 
CCGGCTGCGT GTGGGCGCAT CAGTTACAAT GACATGTTTG AGATGCTGAA ACACATGTCC 
CCGCCTCTGG GGCTGGGGAA GAAATGCCCT GCTCGAGTTG CTTACAAGCG CCTGGTTCGC 
ATGAACATGC CCATCTCCAA CGAGGACATG ACTGTTCACT TCACGTCCAC GCTGATGGCC 
CTCATCCGGA CGGCACTGGA GATCAAGCTG GCCCCAGCTG GGACAAAGCA GCATCAGTGT 
GACGCGGAGT TGAGGAAGGA GATTTCCGTT GTGTGGGCCA ATCTGCCCCA GAAGACTTTG 
GACTTGCTGG TACCACCCCA TAAGCCTGAT GAGATGACAG TGGGGAAGGT TTATGCAGCT 
CTGATGATAT TCGACTTCTA CAAGCAGAAC AAAACCACCA GAGACCAGAT GCAGCAGGCT 
CCTGGAGGCC TCTCCCAGAT GGGTCCTGTG TCCCTGTTCC ACCCTCTGAA GGCCACCCTG 
GAGCAGACAC AGCCGGCTGT GCTCCGAGGA GCCCGGGTTT TCCTTCGACA GAAGAGTTCC 
ACCTCCCTCA GCAATGGCGG GGCCATACAA AACCAAGAGA GTGGCATCAA AGAGTCTGTC 
TCCTGGGGCA CTCAAAGGAC CCAGGATGCA CCCCATGAGG CCAGGCCACC CCTGGAGCGT 
GGCCACTCCA CAGAGATCCC TGTGGGGCGG TCAGGAGCAC TGGCTGTGGA CGTTCAGATG 
CAGAGCATAA CCCGGAGGGG CCCTGATGGG GAGCCCCAGC CTGGGCTGGA GAGCCAGGGT 
CGAGCGGCCT CCATGCCCCG CCTTGCGGCC GAGACTCAGC CCGTCACAGA TGCCAGCCCC 
ATGAAGCGCT CCATCTCCAC GCTGGCCCAG CGGCCCCGTG GGACTCATCT TTGCAGCACC 
ACCCCGGACC GCCCACCCCC TAGCCAGGCG TCGTCGCACC ACCACCACCA CCGCTGCCAC 
CGCCGCAGGG ACAGGAAGCA GAGGTCCCTG GAGAAGGGGC CCAGCCTGTC TGCCGATATG 
GATGGCGCAC CAAGCAGTGC TGTGGGGCCG GGGCTGCCCC CGGGAGAGGG GCCTACAGGC 
TGCCGGCGGG AACGAGAGCG CCGGCAGGAG CGGGGCCGGT CCCAGGAGCG GAGGCAGCCC 
TCATCCTCCT CCTCGGAGAA GCAGCGCTTC TACTCCTGCG ACCGCTTTGG GGGCCGTGAG 
CCCCCGAAGC CCAAGCCCTC CCTCAGCAGC CACCCAACGT CGCCAACAGC TGGCCAGGAG 
CCGGGACCCC ACCCACAGGG CAGTGGTTCC GTGAATGGGA GCCCCTTGCT GTCAACATCT 
GGTGCTAGCA CCCCCGGCCG CGGTGGGCGG AGGCAGCTCC CCCAGACGCC CCTGACTCCC 
CGCCCCAGCA TCACCTACAA GACGGCCAAC TCCTCACCCA TCCACTTCGC CGGGGCTCAG 
ACCAGCCTCC CTGCCTTCTC CCCAGGCCGG CTCAGCCGTG GGCTTTCCGA ACACAACGCC 
CTGCTGCAGA GAGACCCCCT CAGCCAGCCC CTGGCCCCTG GCTCTCGAAT TGGCTCTGAC 
CCTTACCTGG GGCAGCGTCT GGACAGTGAG GCCTCTGTCC ACGCCCTGCC TGAGGACACT 
CTCACTTTCG AGGAGGCTGT GGCCACCAAC TCGGGCCGCT CCTCCAGGAC TTCCTACGTG 
TCCTCCCTGA CCTCCCAGTC TCACCCTCTC CGCCGCGTGC CCAACGGTTA CCACTGCACC 
CTGGGACTCA GCTCGGGTGG CCGAGCACGG CACAGCTACC ACCACCCTGA CCAAGACCAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870605.1
CDS	110..7138
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFVGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPIHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPGSRIGSDPYLGQRLDSEASVHALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_017015116.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015116.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGCAGTGCT GAAGAGAGCG 
GCCACCAAGA AGAGCAGAAA TGACCTGATC CACGCAGAGG AGGGAGAGGA CCGGTTTGCA 
GATCTCTGTG CTGTTGGATC CCCCTTCGCC CGCGCCAGCC TCAAGAGCGG GAAGACAGAG 
AGCTCGTCAT ACTTCCGGAG GAAGGAGAAG ATGTTCCGGT TTTTTATCCG GCGCATGGTG 
AAGGCTCAGA GCTTCTACTG GGTGGTGCTG TGCGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ATTACAACCA GCCGCGGCGG CTTACCACGA CCCTGTATTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGA 
AGCTACTTCC GGTCCTCCTT CAACTGCTTC GACTTTGGGG TCATCGTGGG GAGCGTCTTT 
GAAGTGGTCT GGGCGGCCAT CAAGCCGGGA AGCTCCTTTG GGATCAGTGT GCTGCGGGCC 
CTCCGCCTGC TGAGGATCTT CAAAGTCACG AAGTACTGGA GCTCCCTGCG GAACCTGGTG 
GTGTCCCTGC TGAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCTTGCT CTTCCTGTTC 
ATTGTGGTCT TCGCCCTGCT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTCCAGGAT 
GAGACTCCCA CAACCAACTT CGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC 
CTGACGGGAG AGGACTGGAA TGCAGTGATG TATCACGGGA TCGAATCGCA AGGCGGCGTC 
AGCAAAGGCA TGTTCTCGTC CTTTTACTTC ATTGTCCTGA CACTGTTCGG AAACTACACT 
CTGCTGAATG TCTTTCTGGC CATCGCTGTG GACAACCTGG CCAACGCCCA AGAGCTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA 
GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATCGCCGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTACG GCTGCAGAAC 
CTGCGGGCCA GCTGCGAGGC GCTGTACAGC GAGATGGACC CCGAGGAGCG GCTGCGCTTC 
GCCACTACGC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTGGTGGTG 
GAGCTGGGCC GCGACGGCGC GCGGGGGCCC GTGGGAGGCA AAGCCCGACC TGAGGCTGCG 
GAGGCCCCCG AGGGCGTCGA CCCTCCGCGC AGGCACCACC GGCACCGCGA CAAGGACAAG 
ACCCCCGCGG CGGGGGACCA GGACCGAGCA GAGGCCCCGA AGGCGGAGAG CGGGGAGCCC 
GGTGCCCGGG AGGAGCGGCC GCGGCCGCAC CGCAGCCACA GCAAGGAGGC CGCGGGGCCC 
CCGGAGGCGC GGAGCGAGCG CGGCCGAGGC CCAGGCCCCG AGGGCGGCCG GCGGCACCAC 
CGGCGCGGCT CCCCGGAGGA GGCGGCCGAG CGGGAGCCCC GACGCCACCG CGCGCACCGG 
CACCAGGATC CGAGCAAGGA GTGCGCCGGC GCCAAGGGCG AGCGGCGCGC GCGGCACCGC 
GGCGGCCCCC GAGCGGGGCC CCGGGAGGCG GAGAGCGGGG AGGAGCCGGC GCGGCGGCAC 
CGGGCCCGGC ACAAGGCGCA GCCTGCTCAC GAGGCTGTGG AGAAGGAGAC CACGGAGAAG 
GAGGCCACGG AGAAGGAGGC TGAGATAGTG GAAGCCGACA AGGAAAAGGA GCTCCGGAAC 
CACCAGCCCC GGGAGCCACA CTGTGACCTG GAGACCAGTG GGACTGTGAC TGTGGGTCCC 
ATGCACACAC TGCCCAGCAC CTGTCTCCAG AAGGTGGAGG AACAGCCAGA GGATGCAGAC 
AATCAGCGGA ACGTCACTCG CATGGGCAGT CAGCCCCCAG ACCCGAACAC TATTGTACAT 
ATCCCAGTGA TGCTGACGGG CCCTCTTGGG GAAGCCACGG TCGTTCCCAG TGGTAACGTG 
GACCTGGAAA GCCAAGCAGA GGGGAAGAAG GAGGTGGAAG CGGATGACGT GATGAGGAGC 
GGCCCCCGGC CTATCGTCCC ATACAGCTCC ATGTTCTGTT TAAGCCCCAC CAACCTGCTC 
CGCCGCTTCT GCCACTACAT CGTGACCATG AGGTACTTCG AGGTGGTCAT TCTCGTGGTC 
ATCGCCTTGA GCAGCATCGC CCTGGCTGCT GAGGACCCAG TGCGCACAGA CTCGCCCAGG 
AACAACGCTC TGAAATACCT GGATTACATT TTCACTGGTG TCTTTACCTT TGAGATGGTG 
ATAAAGATGA TCGACTTGGG ACTGCTGCTT CACCCTGGAG CCTATTTCCG GGACTTGTGG 
AACATTCTGG ACTTCATTGT GGTCAGTGGC GCCCTGGTGG CGTTTGCTTT CTCGAGCTTC 
GTGGGAGGAT CCAAAGGGAA AGACATCAAT ACCATCAAGT CTCTGAGAGT CCTTCGTGTC 
CTGCGGCCCC TCAAGACCAT CAAACGGCTG CCCAAGCTCA AGGCTGTGTT TGACTGTGTG 
GTGAACTCCC TGAAGAATGT CCTCAACATC TTGATTGTCT ACATGCTCTT CATGTTCATA 
TTTGCCGTCA TTGCGGTGCA GCTCTTCAAA GGGAAGTTTT TCTACTGCAC AGATGAATCC 
AAGGAGCTGG AGAGGGACTG CAGGGGTCAG TATTTGGATT ATGAGAAGGA GGAAGTGGAA 
GCTCAGCCCA GGCAGTGGAA GAAATACGAC TTTCACTACG ACAATGTGCT CTGGGCTCTG 
CTGACGCTGT TCACAGTGTC CACGGGAGAA GGCTGGCCCA TGGTGCTGAA ACACTCCGTG 
GATGCCACCT ATGAGGAGCA GGGTCCAAGC CCTGGGTACC GCATGGAGCT GTCCATCTTC 
TACGTGGTCT ACTTTGTGGT CTTTCCCTTC TTCTTCGTCA ACATCTTTGT GGCTTTGATC 
ATCATCACCT TCCAGGAGCA GGGGGACAAG GTGATGTCTG AATGCAGCCT GGAGAAGAAC 
GAGAGGGCTT GCATTGACTT CGCCATCAGC GCCAAACCCC TGACACGGTA CATGCCCCAA 
AACCGGCAGT CGTTCCAGTA TAAGACGTGG ACATTTGTGG TCTCCCCGCC CTTTGAATAC 
TTCATCATGG CCATGATAGC CCTCAACACT GTGGTGCTGA TGATGAAGTT CTATGATGCA 
CCCTATGAGT ACGAGCTGAT GCTGAAATGC CTGAACATCG TGTTCACATC CATGTTCTCC 
ATGGAATGCG TGCTGAAGAT CATCGCCTTT GGGGTGCTGA ACTATTTCAG AGATGCCTGG 
AATGTCTTTG ACTTTGTCAC TGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGAACAATT TCATCAACCT CAGCTTCCTC CGCCTCTTTC GAGCTGCGCG GCTGATCAAG 
CTGCTCCGCC AGGGCTACAC CATCCGCATC CTGCTGTGGA CCTTTGTCCA GTCCTTCAAG 
GCCCTGCCCT ACGTGTGTCT GCTCATTGCC ATGCTGTTCT TCATCTACGC CATCATCGGC 
ATGCAGGTGT TTGGGAATAT TGCCCTGGAT GATGACACCA GCATCAACCG CCACAACAAC 
TTCCGGACGT TTTTGCAAGC CCTGATGCTG CTGTTCAGGA GCGCCACGGG GGAGGCCTGG 
CACGAGATCA TGCTGTCCTG CCTGAGCAAC CAGGCCTGTG ATGAGCAGGC CAATGCCACC 
GAGTGTGGAA GTGACTTTGC CTACTTCTAC TTCGTCTCCT TCATCTTCCT GTGCTCCTTT 
CTGATGTTGA ACCTCTTTGT GGCTGTGATC ATGGACAATT TTGAGTACCT CACGCGGGAC 
TCTTCCATCC TAGGTCCTCA CCACTTGGAT GAGTTCATCC GGGTCTGGGC TGAATACGAC 
CCGGCTGCGT GTGGGCGCAT CAGTTACAAT GACATGTTTG AGATGCTGAA ACACATGTCC 
CCGCCTCTGG GGCTGGGGAA GAAATGCCCT GCTCGAGTTG CTTACAAGCG CCTGGTTCGC 
ATGAACATGC CCATCTCCAA CGAGGACATG ACTGTTCACT TCACGTCCAC GCTGATGGCC 
CTCATCCGGA CGGCACTGGA GATCAAGCTG GCCCCAGCTG GGACAAAGCA GCATCAGTGT 
GACGCGGAGT TGAGGAAGGA GATTTCCGTT GTGTGGGCCA ATCTGCCCCA GAAGACTTTG 
GACTTGCTGG TACCACCCCA TAAGCCTGAT GAGATGACAG TGGGGAAGGT TTATGCAGCT 
CTGATGATAT TCGACTTCTA CAAGCAGAAC AAAACCACCA GAGACCAGAT GCAGCAGGCT 
CCTGGAGGCC TCTCCCAGAT GGGTCCTGTG TCCCTGTTCC ACCCTCTGAA GGCCACCCTG 
GAGCAGACAC AGCCGGCTGT GCTCCGAGGA GCCCGGGTTT TCCTTCGACA GAAGAGTTCC 
ACCTCCCTCA GCAATGGCGG GGCCATACAA AACCAAGAGA GTGGCATCAA AGAGTCTGTC 
TCCTGGGGCA CTCAAAGGAC CCAGGATGCA CCCCATGAGG CCAGGCCACC CCTGGAGCGT 
GGCCACTCCA CAGAGATCCC TGTGGGGCGG TCAGGAGCAC TGGCTGTGGA CGTTCAGATG 
CAGAGCATAA CCCGGAGGGG CCCTGATGGG GAGCCCCAGC CTGGGCTGGA GAGCCAGGGT 
CGAGCGGCCT CCATGCCCCG CCTTGCGGCC GAGACTCAGC CCGTCACAGA TGCCAGCCCC 
ATGAAGCGCT CCATCTCCAC GCTGGCCCAG CGGCCCCGTG GGACTCATCT TTGCAGCACC 
ACCCCGGACC GCCCACCCCC TAGCCAGGCG TCGTCGCACC ACCACCACCA CCGCTGCCAC 
CGCCGCAGGG ACAGGAAGCA GAGGTCCCTG GAGAAGGGGC CCAGCCTGTC TGCCGATATG 
GATGGCGCAC CAAGCAGTGC TGTGGGGCCG GGGCTGCCCC CGGGAGAGGG GCCTACAGGC 
TGCCGGCGGG AACGAGAGCG CCGGCAGGAG CGGGGCCGGT CCCAGGAGCG GAGGCAGCCC 
TCATCCTCCT CCTCGGAGAA GCAGCGCTTC TACTCCTGCG ACCGCTTTGG GGGCCGTGAG 
CCCCCGAAGC CCAAGCCCTC CCTCAGCAGC CACCCAACGT CGCCAACAGC TGGCCAGGAG 
CCGGGACCCC ACCCACAGGG CAGTGGTTCC GTGAATGGGA GCCCCTTGCT GTCAACATCT 
GGTGCTAGCA CCCCCGGCCG CGGTGGGCGG AGGCAGCTCC CCCAGACGCC CCTGACTCCC 
CGCCCCAGCA TCACCTACAA GACGGCCAAC TCCTCACCCA TCCACTTCGC CGGGGCTCAG 
ACCAGCCTCC CTGCCTTCTC CCCAGGCCGG CTCAGCCGTG GGCTTTCCGA ACACAACGCC 
CTGCTGCAGA GAGACCCCCT CAGCCAGCCC CTGGCCCCTG GCTCTCGAAT TGGCTCTGAC 
CCTTACCTGG GGCAGCGTCT GGACAGTGAG GCCTCTGTCC ACGCCCTGCC TGAGGACACT 
CTCACTTTCG AGGAGGCTGT GGCCACCAAC TCGGGCCGCT CCTCCAGGAC TTCCTACGTG 
TCCTCCCTGA CCTCCCAGTC TCACCCTCTC CGCCGCGTGC CCAACGGTTA CCACTGCACC 
CTGGGACTCA GCTCGGGTGG CCGAGCACGG CACAGCTACC ACCACCCTGA CCAAGACCAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_017015117.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870606.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X6, mRNA.

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CloneID	OHu98900
Clone ID Related Accession (Same CDS sequence)	XM_017015117.1
Accession Version	XM_017015117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGCAGTGCT GAAGAGAGCG 
GCCACCAAGA AGAGCAGAAA TGACCTGATC CACGCAGAGG AGGGAGAGGA CCGGTTTGCA 
GATCTCTGTG CTGTTGGATC CCCCTTCGCC CGCGCCAGCC TCAAGAGCGG GAAGACAGAG 
AGCTCGTCAT ACTTCCGGAG GAAGGAGAAG ATGTTCCGGT TTTTTATCCG GCGCATGGTG 
AAGGCTCAGA GCTTCTACTG GGTGGTGCTG TGCGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ATTACAACCA GCCGCGGCGG CTTACCACGA CCCTGTATTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGA 
AGCTACTTCC GGTCCTCCTT CAACTGCTTC GACTTTGGGG TCATCGTGGG GAGCGTCTTT 
GAAGTGGTCT GGGCGGCCAT CAAGCCGGGA AGCTCCTTTG GGATCAGTGT GCTGCGGGCC 
CTCCGCCTGC TGAGGATCTT CAAAGTCACG AAGTACTGGA GCTCCCTGCG GAACCTGGTG 
GTGTCCCTGC TGAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCTTGCT CTTCCTGTTC 
ATTGTGGTCT TCGCCCTGCT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTCCAGGAT 
GAGACTCCCA CAACCAACTT CGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC 
CTGACGGGAG AGGACTGGAA TGCAGTGATG TATCACGGGA TCGAATCGCA AGGCGGCGTC 
AGCAAAGGCA TGTTCTCGTC CTTTTACTTC ATTGTCCTGA CACTGTTCGG AAACTACACT 
CTGCTGAATG TCTTTCTGGC CATCGCTGTG GACAACCTGG CCAACGCCCA AGAGCTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA 
GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATCGCCGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTACG GCTGCAGAAC 
CTGCGGGCCA GCTGCGAGGC GCTGTACAGC GAGATGGACC CCGAGGAGCG GCTGCGCTTC 
GCCACTACGC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTGGTGGTG 
GAGCTGGGCC GCGACGGCGC GCGGGGGCCC GTGGGAGGCA AAGCCCGACC TGAGGCTGCG 
GAGGCCCCCG AGGGCGTCGA CCCTCCGCGC AGGCACCACC GGCACCGCGA CAAGGACAAG 
ACCCCCGCGG CGGGGGACCA GGACCGAGCA GAGGCCCCGA AGGCGGAGAG CGGGGAGCCC 
GGTGCCCGGG AGGAGCGGCC GCGGCCGCAC CGCAGCCACA GCAAGGAGGC CGCGGGGCCC 
CCGGAGGCGC GGAGCGAGCG CGGCCGAGGC CCAGGCCCCG AGGGCGGCCG GCGGCACCAC 
CGGCGCGGCT CCCCGGAGGA GGCGGCCGAG CGGGAGCCCC GACGCCACCG CGCGCACCGG 
CACCAGGATC CGAGCAAGGA GTGCGCCGGC GCCAAGGGCG AGCGGCGCGC GCGGCACCGC 
GGCGGCCCCC GAGCGGGGCC CCGGGAGGCG GAGAGCGGGG AGGAGCCGGC GCGGCGGCAC 
CGGGCCCGGC ACAAGGCGCA GCCTGCTCAC GAGGCTGTGG AGAAGGAGAC CACGGAGAAG 
GAGGCCACGG AGAAGGAGGC TGAGATAGTG GAAGCCGACA AGGAAAAGGA GCTCCGGAAC 
CACCAGCCCC GGGAGCCACA CTGTGACCTG GAGACCAGTG GGACTGTGAC TGTGGGTCCC 
ATGCACACAC TGCCCAGCAC CTGTCTCCAG AAGGTGGAGG AACAGCCAGA GGATGCAGAC 
AATCAGCGGA ACGTCACTCG CATGGGCAGT CAGCCCCCAG ACCCGAACAC TATTGTACAT 
ATCCCAGTGA TGCTGACGGG CCCTCTTGGG GAAGCCACGG TCGTTCCCAG TGGTAACGTG 
GACCTGGAAA GCCAAGCAGA GGGGAAGAAG GAGGTGGAAG CGGATGACGT GATGAGGAGC 
GGCCCCCGGC CTATCGTCCC ATACAGCTCC ATGTTCTGTT TAAGCCCCAC CAACCTGCTC 
CGCCGCTTCT GCCACTACAT CGTGACCATG AGGTACTTCG AGGTGGTCAT TCTCGTGGTC 
ATCGCCTTGA GCAGCATCGC CCTGGCTGCT GAGGACCCAG TGCGCACAGA CTCGCCCAGG 
AACAACGCTC TGAAATACCT GGATTACATT TTCACTGGTG TCTTTACCTT TGAGATGGTG 
ATAAAGATGA TCGACTTGGG ACTGCTGCTT CACCCTGGAG CCTATTTCCG GGACTTGTGG 
AACATTCTGG ACTTCATTGT GGTCAGTGGC GCCCTGGTGG CGTTTGCTTT CTCAGGATCC 
AAAGGGAAAG ACATCAATAC CATCAAGTCT CTGAGAGTCC TTCGTGTCCT GCGGCCCCTC 
AAGACCATCA AACGGCTGCC CAAGCTCAAG GCTGTGTTTG ACTGTGTGGT GAACTCCCTG 
AAGAATGTCC TCAACATCTT GATTGTCTAC ATGCTCTTCA TGTTCATATT TGCCGTCATT 
GCGGTGCAGC TCTTCAAAGG GAAGTTTTTC TACTGCACAG ATGAATCCAA GGAGCTGGAG 
AGGGACTGCA GGGGTCAGTA TTTGGATTAT GAGAAGGAGG AAGTGGAAGC TCAGCCCAGG 
CAGTGGAAGA AATACGACTT TCACTACGAC AATGTGCTCT GGGCTCTGCT GACGCTGTTC 
ACAGTGTCCA CGGGAGAAGG CTGGCCCATG GTGCTGAAAC ACTCCGTGGA TGCCACCTAT 
GAGGAGCAGG GTCCAAGCCC TGGGTACCGC ATGGAGCTGT CCATCTTCTA CGTGGTCTAC 
TTTGTGGTCT TTCCCTTCTT CTTCGTCAAC ATCTTTGTGG CTTTGATCAT CATCACCTTC 
CAGGAGCAGG GGGACAAGGT GATGTCTGAA TGCAGCCTGG AGAAGAACGA GAGGGCTTGC 
ATTGACTTCG CCATCAGCGC CAAACCCCTG ACACGGTACA TGCCCCAAAA CCGGCAGTCG 
TTCCAGTATA AGACGTGGAC ATTTGTGGTC TCCCCGCCCT TTGAATACTT CATCATGGCC 
ATGATAGCCC TCAACACTGT GGTGCTGATG ATGAAGTTCT ATGATGCACC CTATGAGTAC 
GAGCTGATGC TGAAATGCCT GAACATCGTG TTCACATCCA TGTTCTCCAT GGAATGCGTG 
CTGAAGATCA TCGCCTTTGG GGTGCTGAAC TATTTCAGAG ATGCCTGGAA TGTCTTTGAC 
TTTGTCACTG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGAAACGAAC 
AATTTCATCA ACCTCAGCTT CCTCCGCCTC TTTCGAGCTG CGCGGCTGAT CAAGCTGCTC 
CGCCAGGGCT ACACCATCCG CATCCTGCTG TGGACCTTTG TCCAGTCCTT CAAGGCCCTG 
CCCTACGTGT GTCTGCTCAT TGCCATGCTG TTCTTCATCT ACGCCATCAT CGGCATGCAG 
GTGTTTGGGA ATATTGCCCT GGATGATGAC ACCAGCATCA ACCGCCACAA CAACTTCCGG 
ACGTTTTTGC AAGCCCTGAT GCTGCTGTTC AGGAGCGCCA CGGGGGAGGC CTGGCACGAG 
ATCATGCTGT CCTGCCTGAG CAACCAGGCC TGTGATGAGC AGGCCAATGC CACCGAGTGT 
GGAAGTGACT TTGCCTACTT CTACTTCGTC TCCTTCATCT TCCTGTGCTC CTTTCTGATG 
TTGAACCTCT TTGTGGCTGT GATCATGGAC AATTTTGAGT ACCTCACGCG GGACTCTTCC 
ATCCTAGGTC CTCACCACTT GGATGAGTTC ATCCGGGTCT GGGCTGAATA CGACCCGGCT 
GCGTGTGGGC GCATCAGTTA CAATGACATG TTTGAGATGC TGAAACACAT GTCCCCGCCT 
CTGGGGCTGG GGAAGAAATG CCCTGCTCGA GTTGCTTACA AGCGCCTGGT TCGCATGAAC 
ATGCCCATCT CCAACGAGGA CATGACTGTT CACTTCACGT CCACGCTGAT GGCCCTCATC 
CGGACGGCAC TGGAGATCAA GCTGGCCCCA GCTGGGACAA AGCAGCATCA GTGTGACGCG 
GAGTTGAGGA AGGAGATTTC CGTTGTGTGG GCCAATCTGC CCCAGAAGAC TTTGGACTTG 
CTGGTACCAC CCCATAAGCC TGATGAGATG ACAGTGGGGA AGGTTTATGC AGCTCTGATG 
ATATTCGACT TCTACAAGCA GAACAAAACC ACCAGAGACC AGATGCAGCA GGCTCCTGGA 
GGCCTCTCCC AGATGGGTCC TGTGTCCCTG TTCCACCCTC TGAAGGCCAC CCTGGAGCAG 
ACACAGCCGG CTGTGCTCCG AGGAGCCCGG GTTTTCCTTC GACAGAAGAG TTCCACCTCC 
CTCAGCAATG GCGGGGCCAT ACAAAACCAA GAGAGTGGCA TCAAAGAGTC TGTCTCCTGG 
GGCACTCAAA GGACCCAGGA TGCACCCCAT GAGGCCAGGC CACCCCTGGA GCGTGGCCAC 
TCCACAGAGA TCCCTGTGGG GCGGTCAGGA GCACTGGCTG TGGACGTTCA GATGCAGAGC 
ATAACCCGGA GGGGCCCTGA TGGGGAGCCC CAGCCTGGGC TGGAGAGCCA GGGTCGAGCG 
GCCTCCATGC CCCGCCTTGC GGCCGAGACT CAGCCCGTCA CAGATGCCAG CCCCATGAAG 
CGCTCCATCT CCACGCTGGC CCAGCGGCCC CGTGGGACTC ATCTTTGCAG CACCACCCCG 
GACCGCCCAC CCCCTAGCCA GGCGTCGTCG CACCACCACC ACCACCGCTG CCACCGCCGC 
AGGGACAGGA AGCAGAGGTC CCTGGAGAAG GGGCCCAGCC TGTCTGCCGA TATGGATGGC 
GCACCAAGCA GTGCTGTGGG GCCGGGGCTG CCCCCGGGAG AGGGGCCTAC AGGCTGCCGG 
CGGGAACGAG AGCGCCGGCA GGAGCGGGGC CGGTCCCAGG AGCGGAGGCA GCCCTCATCC 
TCCTCCTCGG AGAAGCAGCG CTTCTACTCC TGCGACCGCT TTGGGGGCCG TGAGCCCCCG 
AAGCCCAAGC CCTCCCTCAG CAGCCACCCA ACGTCGCCAA CAGCTGGCCA GGAGCCGGGA 
CCCCACCCAC AGGGCAGTGG TTCCGTGAAT GGGAGCCCCT TGCTGTCAAC ATCTGGTGCT 
AGCACCCCCG GCCGCGGTGG GCGGAGGCAG CTCCCCCAGA CGCCCCTGAC TCCCCGCCCC 
AGCATCACCT ACAAGACGGC CAACTCCTCA CCCATCCACT TCGCCGGGGC TCAGACCAGC 
CTCCCTGCCT TCTCCCCAGG CCGGCTCAGC CGTGGGCTTT CCGAACACAA CGCCCTGCTG 
CAGAGAGACC CCCTCAGCCA GCCCCTGGCC CCTGGCTCTC GAATTGGCTC TGACCCTTAC 
CTGGGGCAGC GTCTGGACAG TGAGGCCTCT GTCCACGCCC TGCCTGAGGA CACTCTCACT 
TTCGAGGAGG CTGTGGCCAC CAACTCGGGC CGCTCCTCCA GGACTTCCTA CGTGTCCTCC 
CTGACCTCCC AGTCTCACCC TCTCCGCCGC GTGCCCAACG GTTACCACTG CACCCTGGGA 
CTCAGCTCGG GTGGCCGAGC ACGGCACAGC TACCACCACC CTGACCAAGA CCACTGGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870606.1
CDS	109..7131
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPIHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPGSRIGSDPYLGQRLDSEASVHALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_017015117.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015117.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGCAGTGCT GAAGAGAGCG 
GCCACCAAGA AGAGCAGAAA TGACCTGATC CACGCAGAGG AGGGAGAGGA CCGGTTTGCA 
GATCTCTGTG CTGTTGGATC CCCCTTCGCC CGCGCCAGCC TCAAGAGCGG GAAGACAGAG 
AGCTCGTCAT ACTTCCGGAG GAAGGAGAAG ATGTTCCGGT TTTTTATCCG GCGCATGGTG 
AAGGCTCAGA GCTTCTACTG GGTGGTGCTG TGCGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ATTACAACCA GCCGCGGCGG CTTACCACGA CCCTGTATTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGA 
AGCTACTTCC GGTCCTCCTT CAACTGCTTC GACTTTGGGG TCATCGTGGG GAGCGTCTTT 
GAAGTGGTCT GGGCGGCCAT CAAGCCGGGA AGCTCCTTTG GGATCAGTGT GCTGCGGGCC 
CTCCGCCTGC TGAGGATCTT CAAAGTCACG AAGTACTGGA GCTCCCTGCG GAACCTGGTG 
GTGTCCCTGC TGAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCTTGCT CTTCCTGTTC 
ATTGTGGTCT TCGCCCTGCT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTCCAGGAT 
GAGACTCCCA CAACCAACTT CGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC 
CTGACGGGAG AGGACTGGAA TGCAGTGATG TATCACGGGA TCGAATCGCA AGGCGGCGTC 
AGCAAAGGCA TGTTCTCGTC CTTTTACTTC ATTGTCCTGA CACTGTTCGG AAACTACACT 
CTGCTGAATG TCTTTCTGGC CATCGCTGTG GACAACCTGG CCAACGCCCA AGAGCTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA 
GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATCGCCGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTACG GCTGCAGAAC 
CTGCGGGCCA GCTGCGAGGC GCTGTACAGC GAGATGGACC CCGAGGAGCG GCTGCGCTTC 
GCCACTACGC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTGGTGGTG 
GAGCTGGGCC GCGACGGCGC GCGGGGGCCC GTGGGAGGCA AAGCCCGACC TGAGGCTGCG 
GAGGCCCCCG AGGGCGTCGA CCCTCCGCGC AGGCACCACC GGCACCGCGA CAAGGACAAG 
ACCCCCGCGG CGGGGGACCA GGACCGAGCA GAGGCCCCGA AGGCGGAGAG CGGGGAGCCC 
GGTGCCCGGG AGGAGCGGCC GCGGCCGCAC CGCAGCCACA GCAAGGAGGC CGCGGGGCCC 
CCGGAGGCGC GGAGCGAGCG CGGCCGAGGC CCAGGCCCCG AGGGCGGCCG GCGGCACCAC 
CGGCGCGGCT CCCCGGAGGA GGCGGCCGAG CGGGAGCCCC GACGCCACCG CGCGCACCGG 
CACCAGGATC CGAGCAAGGA GTGCGCCGGC GCCAAGGGCG AGCGGCGCGC GCGGCACCGC 
GGCGGCCCCC GAGCGGGGCC CCGGGAGGCG GAGAGCGGGG AGGAGCCGGC GCGGCGGCAC 
CGGGCCCGGC ACAAGGCGCA GCCTGCTCAC GAGGCTGTGG AGAAGGAGAC CACGGAGAAG 
GAGGCCACGG AGAAGGAGGC TGAGATAGTG GAAGCCGACA AGGAAAAGGA GCTCCGGAAC 
CACCAGCCCC GGGAGCCACA CTGTGACCTG GAGACCAGTG GGACTGTGAC TGTGGGTCCC 
ATGCACACAC TGCCCAGCAC CTGTCTCCAG AAGGTGGAGG AACAGCCAGA GGATGCAGAC 
AATCAGCGGA ACGTCACTCG CATGGGCAGT CAGCCCCCAG ACCCGAACAC TATTGTACAT 
ATCCCAGTGA TGCTGACGGG CCCTCTTGGG GAAGCCACGG TCGTTCCCAG TGGTAACGTG 
GACCTGGAAA GCCAAGCAGA GGGGAAGAAG GAGGTGGAAG CGGATGACGT GATGAGGAGC 
GGCCCCCGGC CTATCGTCCC ATACAGCTCC ATGTTCTGTT TAAGCCCCAC CAACCTGCTC 
CGCCGCTTCT GCCACTACAT CGTGACCATG AGGTACTTCG AGGTGGTCAT TCTCGTGGTC 
ATCGCCTTGA GCAGCATCGC CCTGGCTGCT GAGGACCCAG TGCGCACAGA CTCGCCCAGG 
AACAACGCTC TGAAATACCT GGATTACATT TTCACTGGTG TCTTTACCTT TGAGATGGTG 
ATAAAGATGA TCGACTTGGG ACTGCTGCTT CACCCTGGAG CCTATTTCCG GGACTTGTGG 
AACATTCTGG ACTTCATTGT GGTCAGTGGC GCCCTGGTGG CGTTTGCTTT CTCAGGATCC 
AAAGGGAAAG ACATCAATAC CATCAAGTCT CTGAGAGTCC TTCGTGTCCT GCGGCCCCTC 
AAGACCATCA AACGGCTGCC CAAGCTCAAG GCTGTGTTTG ACTGTGTGGT GAACTCCCTG 
AAGAATGTCC TCAACATCTT GATTGTCTAC ATGCTCTTCA TGTTCATATT TGCCGTCATT 
GCGGTGCAGC TCTTCAAAGG GAAGTTTTTC TACTGCACAG ATGAATCCAA GGAGCTGGAG 
AGGGACTGCA GGGGTCAGTA TTTGGATTAT GAGAAGGAGG AAGTGGAAGC TCAGCCCAGG 
CAGTGGAAGA AATACGACTT TCACTACGAC AATGTGCTCT GGGCTCTGCT GACGCTGTTC 
ACAGTGTCCA CGGGAGAAGG CTGGCCCATG GTGCTGAAAC ACTCCGTGGA TGCCACCTAT 
GAGGAGCAGG GTCCAAGCCC TGGGTACCGC ATGGAGCTGT CCATCTTCTA CGTGGTCTAC 
TTTGTGGTCT TTCCCTTCTT CTTCGTCAAC ATCTTTGTGG CTTTGATCAT CATCACCTTC 
CAGGAGCAGG GGGACAAGGT GATGTCTGAA TGCAGCCTGG AGAAGAACGA GAGGGCTTGC 
ATTGACTTCG CCATCAGCGC CAAACCCCTG ACACGGTACA TGCCCCAAAA CCGGCAGTCG 
TTCCAGTATA AGACGTGGAC ATTTGTGGTC TCCCCGCCCT TTGAATACTT CATCATGGCC 
ATGATAGCCC TCAACACTGT GGTGCTGATG ATGAAGTTCT ATGATGCACC CTATGAGTAC 
GAGCTGATGC TGAAATGCCT GAACATCGTG TTCACATCCA TGTTCTCCAT GGAATGCGTG 
CTGAAGATCA TCGCCTTTGG GGTGCTGAAC TATTTCAGAG ATGCCTGGAA TGTCTTTGAC 
TTTGTCACTG TGTTGGGAAG TATTACTGAT ATTTTAGTAA CAGAGATTGC GGAAACGAAC 
AATTTCATCA ACCTCAGCTT CCTCCGCCTC TTTCGAGCTG CGCGGCTGAT CAAGCTGCTC 
CGCCAGGGCT ACACCATCCG CATCCTGCTG TGGACCTTTG TCCAGTCCTT CAAGGCCCTG 
CCCTACGTGT GTCTGCTCAT TGCCATGCTG TTCTTCATCT ACGCCATCAT CGGCATGCAG 
GTGTTTGGGA ATATTGCCCT GGATGATGAC ACCAGCATCA ACCGCCACAA CAACTTCCGG 
ACGTTTTTGC AAGCCCTGAT GCTGCTGTTC AGGAGCGCCA CGGGGGAGGC CTGGCACGAG 
ATCATGCTGT CCTGCCTGAG CAACCAGGCC TGTGATGAGC AGGCCAATGC CACCGAGTGT 
GGAAGTGACT TTGCCTACTT CTACTTCGTC TCCTTCATCT TCCTGTGCTC CTTTCTGATG 
TTGAACCTCT TTGTGGCTGT GATCATGGAC AATTTTGAGT ACCTCACGCG GGACTCTTCC 
ATCCTAGGTC CTCACCACTT GGATGAGTTC ATCCGGGTCT GGGCTGAATA CGACCCGGCT 
GCGTGTGGGC GCATCAGTTA CAATGACATG TTTGAGATGC TGAAACACAT GTCCCCGCCT 
CTGGGGCTGG GGAAGAAATG CCCTGCTCGA GTTGCTTACA AGCGCCTGGT TCGCATGAAC 
ATGCCCATCT CCAACGAGGA CATGACTGTT CACTTCACGT CCACGCTGAT GGCCCTCATC 
CGGACGGCAC TGGAGATCAA GCTGGCCCCA GCTGGGACAA AGCAGCATCA GTGTGACGCG 
GAGTTGAGGA AGGAGATTTC CGTTGTGTGG GCCAATCTGC CCCAGAAGAC TTTGGACTTG 
CTGGTACCAC CCCATAAGCC TGATGAGATG ACAGTGGGGA AGGTTTATGC AGCTCTGATG 
ATATTCGACT TCTACAAGCA GAACAAAACC ACCAGAGACC AGATGCAGCA GGCTCCTGGA 
GGCCTCTCCC AGATGGGTCC TGTGTCCCTG TTCCACCCTC TGAAGGCCAC CCTGGAGCAG 
ACACAGCCGG CTGTGCTCCG AGGAGCCCGG GTTTTCCTTC GACAGAAGAG TTCCACCTCC 
CTCAGCAATG GCGGGGCCAT ACAAAACCAA GAGAGTGGCA TCAAAGAGTC TGTCTCCTGG 
GGCACTCAAA GGACCCAGGA TGCACCCCAT GAGGCCAGGC CACCCCTGGA GCGTGGCCAC 
TCCACAGAGA TCCCTGTGGG GCGGTCAGGA GCACTGGCTG TGGACGTTCA GATGCAGAGC 
ATAACCCGGA GGGGCCCTGA TGGGGAGCCC CAGCCTGGGC TGGAGAGCCA GGGTCGAGCG 
GCCTCCATGC CCCGCCTTGC GGCCGAGACT CAGCCCGTCA CAGATGCCAG CCCCATGAAG 
CGCTCCATCT CCACGCTGGC CCAGCGGCCC CGTGGGACTC ATCTTTGCAG CACCACCCCG 
GACCGCCCAC CCCCTAGCCA GGCGTCGTCG CACCACCACC ACCACCGCTG CCACCGCCGC 
AGGGACAGGA AGCAGAGGTC CCTGGAGAAG GGGCCCAGCC TGTCTGCCGA TATGGATGGC 
GCACCAAGCA GTGCTGTGGG GCCGGGGCTG CCCCCGGGAG AGGGGCCTAC AGGCTGCCGG 
CGGGAACGAG AGCGCCGGCA GGAGCGGGGC CGGTCCCAGG AGCGGAGGCA GCCCTCATCC 
TCCTCCTCGG AGAAGCAGCG CTTCTACTCC TGCGACCGCT TTGGGGGCCG TGAGCCCCCG 
AAGCCCAAGC CCTCCCTCAG CAGCCACCCA ACGTCGCCAA CAGCTGGCCA GGAGCCGGGA 
CCCCACCCAC AGGGCAGTGG TTCCGTGAAT GGGAGCCCCT TGCTGTCAAC ATCTGGTGCT 
AGCACCCCCG GCCGCGGTGG GCGGAGGCAG CTCCCCCAGA CGCCCCTGAC TCCCCGCCCC 
AGCATCACCT ACAAGACGGC CAACTCCTCA CCCATCCACT TCGCCGGGGC TCAGACCAGC 
CTCCCTGCCT TCTCCCCAGG CCGGCTCAGC CGTGGGCTTT CCGAACACAA CGCCCTGCTG 
CAGAGAGACC CCCTCAGCCA GCCCCTGGCC CCTGGCTCTC GAATTGGCTC TGACCCTTAC 
CTGGGGCAGC GTCTGGACAG TGAGGCCTCT GTCCACGCCC TGCCTGAGGA CACTCTCACT 
TTCGAGGAGG CTGTGGCCAC CAACTCGGGC CGCTCCTCCA GGACTTCCTA CGTGTCCTCC 
CTGACCTCCC AGTCTCACCC TCTCCGCCGC GTGCCCAACG GTTACCACTG CACCCTGGGA 
CTCAGCTCGG GTGGCCGAGC ACGGCACAGC TACCACCACC CTGACCAAGA CCACTGGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_017015118.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870607.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X8, mRNA.

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CloneID	OHu98901
Clone ID Related Accession (Same CDS sequence)	XM_017015118.1
Accession Version	XM_017015118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCGCA GCAGAACTCG 
GCCAAGGCGC GCTCGGTGTG GGAGCAGCGG GCCAGCCAGC TACGGCTGCA GAACCTGCGG 
GCCAGCTGCG AGGCGCTGTA CAGCGAGATG GACCCCGAGG AGCGGCTGCG CTTCGCCACT 
ACGCGCCACC TGCGGCCCGA CATGAAGACG CACCTGGACC GGCCGCTGGT GGTGGAGCTG 
GGCCGCGACG GCGCGCGGGG GCCCGTGGGA GGCAAAGCCC GACCTGAGGC TGCGGAGGCC 
CCCGAGGGCG TCGACCCTCC GCGCAGGCAC CACCGGCACC GCGACAAGGA CAAGACCCCC 
GCGGCGGGGG ACCAGGACCG AGCAGAGGCC CCGAAGGCGG AGAGCGGGGA GCCCGGTGCC 
CGGGAGGAGC GGCCGCGGCC GCACCGCAGC CACAGCAAGG AGGCCGCGGG GCCCCCGGAG 
GCGCGGAGCG AGCGCGGCCG AGGCCCAGGC CCCGAGGGCG GCCGGCGGCA CCACCGGCGC 
GGCTCCCCGG AGGAGGCGGC CGAGCGGGAG CCCCGACGCC ACCGCGCGCA CCGGCACCAG 
GATCCGAGCA AGGAGTGCGC CGGCGCCAAG GGCGAGCGGC GCGCGCGGCA CCGCGGCGGC 
CCCCGAGCGG GGCCCCGGGA GGCGGAGAGC GGGGAGGAGC CGGCGCGGCG GCACCGGGCC 
CGGCACAAGG CGCAGCCTGC TCACGAGGCT GTGGAGAAGG AGACCACGGA GAAGGAGGCC 
ACGGAGAAGG AGGCTGAGAT AGTGGAAGCC GACAAGGAAA AGGAGCTCCG GAACCACCAG 
CCCCGGGAGC CACACTGTGA CCTGGAGACC AGTGGGACTG TGACTGTGGG TCCCATGCAC 
ACACTGCCCA GCACCTGTCT CCAGAAGGTG GAGGAACAGC CAGAGGATGC AGACAATCAG 
CGGAACGTCA CTCGCATGGG CAGTCAGCCC CCAGACCCGA ACACTATTGT ACATATCCCA 
GTGATGCTGA CGGGCCCTCT TGGGGAAGCC ACGGTCGTTC CCAGTGGTAA CGTGGACCTG 
GAAAGCCAAG CAGAGGGGAA GAAGGAGGTG GAAGCGGATG ACGTGATGAG GAGCGGCCCC 
CGGCCTATCG TCCCATACAG CTCCATGTTC TGTTTAAGCC CCACCAACCT GCTCCGCCGC 
TTCTGCCACT ACATCGTGAC CATGAGGTAC TTCGAGGTGG TCATTCTCGT GGTCATCGCC 
TTGAGCAGCA TCGCCCTGGC TGCTGAGGAC CCAGTGCGCA CAGACTCGCC CAGGAACAAC 
GCTCTGAAAT ACCTGGATTA CATTTTCACT GGTGTCTTTA CCTTTGAGAT GGTGATAAAG 
ATGATCGACT TGGGACTGCT GCTTCACCCT GGAGCCTATT TCCGGGACTT GTGGAACATT 
CTGGACTTCA TTGTGGTCAG TGGCGCCCTG GTGGCGTTTG CTTTCTCAGG ATCCAAAGGG 
AAAGACATCA ATACCATCAA GTCTCTGAGA GTCCTTCGTG TCCTGCGGCC CCTCAAGACC 
ATCAAACGGC TGCCCAAGCT CAAGGCTGTG TTTGACTGTG TGGTGAACTC CCTGAAGAAT 
GTCCTCAACA TCTTGATTGT CTACATGCTC TTCATGTTCA TATTTGCCGT CATTGCGGTG 
CAGCTCTTCA AAGGGAAGTT TTTCTACTGC ACAGATGAAT CCAAGGAGCT GGAGAGGGAC 
TGCAGGGGTC AGTATTTGGA TTATGAGAAG GAGGAAGTGG AAGCTCAGCC CAGGCAGTGG 
AAGAAATACG ACTTTCACTA CGACAATGTG CTCTGGGCTC TGCTGACGCT GTTCACAGTG 
TCCACGGGAG AAGGCTGGCC CATGGTGCTG AAACACTCCG TGGATGCCAC CTATGAGGAG 
CAGGGTCCAA GCCCTGGGTA CCGCATGGAG CTGTCCATCT TCTACGTGGT CTACTTTGTG 
GTCTTTCCCT TCTTCTTCGT CAACATCTTT GTGGCTTTGA TCATCATCAC CTTCCAGGAG 
CAGGGGGACA AGGTGATGTC TGAATGCAGC CTGGAGAAGA ACGAGAGGGC TTGCATTGAC 
TTCGCCATCA GCGCCAAACC CCTGACACGG TACATGCCCC AAAACCGGCA GTCGTTCCAG 
TATAAGACGT GGACATTTGT GGTCTCCCCG CCCTTTGAAT ACTTCATCAT GGCCATGATA 
GCCCTCAACA CTGTGGTGCT GATGATGAAG TTCTATGATG CACCCTATGA GTACGAGCTG 
ATGCTGAAAT GCCTGAACAT CGTGTTCACA TCCATGTTCT CCATGGAATG CGTGCTGAAG 
ATCATCGCCT TTGGGGTGCT GAACTATTTC AGAGATGCCT GGAATGTCTT TGACTTTGTC 
ACTGTGTTGG GAAGTATTAC TGATATTTTA GTAACAGAGA TTGCGGAAAC GAACAATTTC 
ATCAACCTCA GCTTCCTCCG CCTCTTTCGA GCTGCGCGGC TGATCAAGCT GCTCCGCCAG 
GGCTACACCA TCCGCATCCT GCTGTGGACC TTTGTCCAGT CCTTCAAGGC CCTGCCCTAC 
GTGTGTCTGC TCATTGCCAT GCTGTTCTTC ATCTACGCCA TCATCGGCAT GCAGGTGTTT 
GGGAATATTG CCCTGGATGA TGACACCAGC ATCAACCGCC ACAACAACTT CCGGACGTTT 
TTGCAAGCCC TGATGCTGCT GTTCAGGAGC GCCACGGGGG AGGCCTGGCA CGAGATCATG 
CTGTCCTGCC TGAGCAACCA GGCCTGTGAT GAGCAGGCCA ATGCCACCGA GTGTGGAAGT 
GACTTTGCCT ACTTCTACTT CGTCTCCTTC ATCTTCCTGT GCTCCTTTCT GATGTTGAAC 
CTCTTTGTGG CTGTGATCAT GGACAATTTT GAGTACCTCA CGCGGGACTC TTCCATCCTA 
GGTCCTCACC ACTTGGATGA GTTCATCCGG GTCTGGGCTG AATACGACCC GGCTGCGTGT 
GGGCGCATCA GTTACAATGA CATGTTTGAG ATGCTGAAAC ACATGTCCCC GCCTCTGGGG 
CTGGGGAAGA AATGCCCTGC TCGAGTTGCT TACAAGCGCC TGGTTCGCAT GAACATGCCC 
ATCTCCAACG AGGACATGAC TGTTCACTTC ACGTCCACGC TGATGGCCCT CATCCGGACG 
GCACTGGAGA TCAAGCTGGC CCCAGCTGGG ACAAAGCAGC ATCAGTGTGA CGCGGAGTTG 
AGGAAGGAGA TTTCCGTTGT GTGGGCCAAT CTGCCCCAGA AGACTTTGGA CTTGCTGGTA 
CCACCCCATA AGCCTGATGA GATGACAGTG GGGAAGGTTT ATGCAGCTCT GATGATATTC 
GACTTCTACA AGCAGAACAA AACCACCAGA GACCAGATGC AGCAGGCTCC TGGAGGCCTC 
TCCCAGATGG GTCCTGTGTC CCTGTTCCAC CCTCTGAAGG CCACCCTGGA GCAGACACAG 
CCGGCTGTGC TCCGAGGAGC CCGGGTTTTC CTTCGACAGA AGAGTTCCAC CTCCCTCAGC 
AATGGCGGGG CCATACAAAA CCAAGAGAGT GGCATCAAAG AGTCTGTCTC CTGGGGCACT 
CAAAGGACCC AGGATGCACC CCATGAGGCC AGGCCACCCC TGGAGCGTGG CCACTCCACA 
GAGATCCCTG TGGGGCGGTC AGGAGCACTG GCTGTGGACG TTCAGATGCA GAGCATAACC 
CGGAGGGGCC CTGATGGGGA GCCCCAGCCT GGGCTGGAGA GCCAGGGTCG AGCGGCCTCC 
ATGCCCCGCC TTGCGGCCGA GACTCAGCCC GTCACAGATG CCAGCCCCAT GAAGCGCTCC 
ATCTCCACGC TGGCCCAGCG GCCCCGTGGG ACTCATCTTT GCAGCACCAC CCCGGACCGC 
CCACCCCCTA GCCAGGCGTC GTCGCACCAC CACCACCACC GCTGCCACCG CCGCAGGGAC 
AGGAAGCAGA GGTCCCTGGA GAAGGGGCCC AGCCTGTCTG CCGATATGGA TGGCGCACCA 
AGCAGTGCTG TGGGGCCGGG GCTGCCCCCG GGAGAGGGGC CTACAGGCTG CCGGCGGGAA 
CGAGAGCGCC GGCAGGAGCG GGGCCGGTCC CAGGAGCGGA GGCAGCCCTC ATCCTCCTCC 
TCGGAGAAGC AGCGCTTCTA CTCCTGCGAC CGCTTTGGGG GCCGTGAGCC CCCGAAGCCC 
AAGCCCTCCC TCAGCAGCCA CCCAACGTCG CCAACAGCTG GCCAGGAGCC GGGACCCCAC 
CCACAGGGCA GTGGTTCCGT GAATGGGAGC CCCTTGCTGT CAACATCTGG TGCTAGCACC 
CCCGGCCGCG GTGGGCGGAG GCAGCTCCCC CAGACGCCCC TGACTCCCCG CCCCAGCATC 
ACCTACAAGA CGGCCAACTC CTCACCCATC CACTTCGCCG GGGCTCAGAC CAGCCTCCCT 
GCCTTCTCCC CAGGCCGGCT CAGCCGTGGG CTTTCCGAAC ACAACGCCCT GCTGCAGAGA 
GACCCCCTCA GCCAGCCCCT GGCCCCTGGC TCTCGAATTG GCTCTGACCC TTACCTGGGG 
CAGCGTCTGG ACAGTGAGGC CTCTGTCCAC GCCCTGCCTG AGGACACTCT CACTTTCGAG 
GAGGCTGTGG CCACCAACTC GGGCCGCTCC TCCAGGACTT CCTACGTGTC CTCCCTGACC 
TCCCAGTCTC ACCCTCTCCG CCGCGTGCCC AACGGTTACC ACTGCACCCT GGGACTCAGC 
TCGGGTGGCC GAGCACGGCA CAGCTACCAC CACCCTGACC AAGACCACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870607.1
CDS	109..7125
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAAQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPIHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPGSRIGSDPYLGQRLDSEASVHALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_017015118.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015118.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCGCA GCAGAACTCG 
GCCAAGGCGC GCTCGGTGTG GGAGCAGCGG GCCAGCCAGC TACGGCTGCA GAACCTGCGG 
GCCAGCTGCG AGGCGCTGTA CAGCGAGATG GACCCCGAGG AGCGGCTGCG CTTCGCCACT 
ACGCGCCACC TGCGGCCCGA CATGAAGACG CACCTGGACC GGCCGCTGGT GGTGGAGCTG 
GGCCGCGACG GCGCGCGGGG GCCCGTGGGA GGCAAAGCCC GACCTGAGGC TGCGGAGGCC 
CCCGAGGGCG TCGACCCTCC GCGCAGGCAC CACCGGCACC GCGACAAGGA CAAGACCCCC 
GCGGCGGGGG ACCAGGACCG AGCAGAGGCC CCGAAGGCGG AGAGCGGGGA GCCCGGTGCC 
CGGGAGGAGC GGCCGCGGCC GCACCGCAGC CACAGCAAGG AGGCCGCGGG GCCCCCGGAG 
GCGCGGAGCG AGCGCGGCCG AGGCCCAGGC CCCGAGGGCG GCCGGCGGCA CCACCGGCGC 
GGCTCCCCGG AGGAGGCGGC CGAGCGGGAG CCCCGACGCC ACCGCGCGCA CCGGCACCAG 
GATCCGAGCA AGGAGTGCGC CGGCGCCAAG GGCGAGCGGC GCGCGCGGCA CCGCGGCGGC 
CCCCGAGCGG GGCCCCGGGA GGCGGAGAGC GGGGAGGAGC CGGCGCGGCG GCACCGGGCC 
CGGCACAAGG CGCAGCCTGC TCACGAGGCT GTGGAGAAGG AGACCACGGA GAAGGAGGCC 
ACGGAGAAGG AGGCTGAGAT AGTGGAAGCC GACAAGGAAA AGGAGCTCCG GAACCACCAG 
CCCCGGGAGC CACACTGTGA CCTGGAGACC AGTGGGACTG TGACTGTGGG TCCCATGCAC 
ACACTGCCCA GCACCTGTCT CCAGAAGGTG GAGGAACAGC CAGAGGATGC AGACAATCAG 
CGGAACGTCA CTCGCATGGG CAGTCAGCCC CCAGACCCGA ACACTATTGT ACATATCCCA 
GTGATGCTGA CGGGCCCTCT TGGGGAAGCC ACGGTCGTTC CCAGTGGTAA CGTGGACCTG 
GAAAGCCAAG CAGAGGGGAA GAAGGAGGTG GAAGCGGATG ACGTGATGAG GAGCGGCCCC 
CGGCCTATCG TCCCATACAG CTCCATGTTC TGTTTAAGCC CCACCAACCT GCTCCGCCGC 
TTCTGCCACT ACATCGTGAC CATGAGGTAC TTCGAGGTGG TCATTCTCGT GGTCATCGCC 
TTGAGCAGCA TCGCCCTGGC TGCTGAGGAC CCAGTGCGCA CAGACTCGCC CAGGAACAAC 
GCTCTGAAAT ACCTGGATTA CATTTTCACT GGTGTCTTTA CCTTTGAGAT GGTGATAAAG 
ATGATCGACT TGGGACTGCT GCTTCACCCT GGAGCCTATT TCCGGGACTT GTGGAACATT 
CTGGACTTCA TTGTGGTCAG TGGCGCCCTG GTGGCGTTTG CTTTCTCAGG ATCCAAAGGG 
AAAGACATCA ATACCATCAA GTCTCTGAGA GTCCTTCGTG TCCTGCGGCC CCTCAAGACC 
ATCAAACGGC TGCCCAAGCT CAAGGCTGTG TTTGACTGTG TGGTGAACTC CCTGAAGAAT 
GTCCTCAACA TCTTGATTGT CTACATGCTC TTCATGTTCA TATTTGCCGT CATTGCGGTG 
CAGCTCTTCA AAGGGAAGTT TTTCTACTGC ACAGATGAAT CCAAGGAGCT GGAGAGGGAC 
TGCAGGGGTC AGTATTTGGA TTATGAGAAG GAGGAAGTGG AAGCTCAGCC CAGGCAGTGG 
AAGAAATACG ACTTTCACTA CGACAATGTG CTCTGGGCTC TGCTGACGCT GTTCACAGTG 
TCCACGGGAG AAGGCTGGCC CATGGTGCTG AAACACTCCG TGGATGCCAC CTATGAGGAG 
CAGGGTCCAA GCCCTGGGTA CCGCATGGAG CTGTCCATCT TCTACGTGGT CTACTTTGTG 
GTCTTTCCCT TCTTCTTCGT CAACATCTTT GTGGCTTTGA TCATCATCAC CTTCCAGGAG 
CAGGGGGACA AGGTGATGTC TGAATGCAGC CTGGAGAAGA ACGAGAGGGC TTGCATTGAC 
TTCGCCATCA GCGCCAAACC CCTGACACGG TACATGCCCC AAAACCGGCA GTCGTTCCAG 
TATAAGACGT GGACATTTGT GGTCTCCCCG CCCTTTGAAT ACTTCATCAT GGCCATGATA 
GCCCTCAACA CTGTGGTGCT GATGATGAAG TTCTATGATG CACCCTATGA GTACGAGCTG 
ATGCTGAAAT GCCTGAACAT CGTGTTCACA TCCATGTTCT CCATGGAATG CGTGCTGAAG 
ATCATCGCCT TTGGGGTGCT GAACTATTTC AGAGATGCCT GGAATGTCTT TGACTTTGTC 
ACTGTGTTGG GAAGTATTAC TGATATTTTA GTAACAGAGA TTGCGGAAAC GAACAATTTC 
ATCAACCTCA GCTTCCTCCG CCTCTTTCGA GCTGCGCGGC TGATCAAGCT GCTCCGCCAG 
GGCTACACCA TCCGCATCCT GCTGTGGACC TTTGTCCAGT CCTTCAAGGC CCTGCCCTAC 
GTGTGTCTGC TCATTGCCAT GCTGTTCTTC ATCTACGCCA TCATCGGCAT GCAGGTGTTT 
GGGAATATTG CCCTGGATGA TGACACCAGC ATCAACCGCC ACAACAACTT CCGGACGTTT 
TTGCAAGCCC TGATGCTGCT GTTCAGGAGC GCCACGGGGG AGGCCTGGCA CGAGATCATG 
CTGTCCTGCC TGAGCAACCA GGCCTGTGAT GAGCAGGCCA ATGCCACCGA GTGTGGAAGT 
GACTTTGCCT ACTTCTACTT CGTCTCCTTC ATCTTCCTGT GCTCCTTTCT GATGTTGAAC 
CTCTTTGTGG CTGTGATCAT GGACAATTTT GAGTACCTCA CGCGGGACTC TTCCATCCTA 
GGTCCTCACC ACTTGGATGA GTTCATCCGG GTCTGGGCTG AATACGACCC GGCTGCGTGT 
GGGCGCATCA GTTACAATGA CATGTTTGAG ATGCTGAAAC ACATGTCCCC GCCTCTGGGG 
CTGGGGAAGA AATGCCCTGC TCGAGTTGCT TACAAGCGCC TGGTTCGCAT GAACATGCCC 
ATCTCCAACG AGGACATGAC TGTTCACTTC ACGTCCACGC TGATGGCCCT CATCCGGACG 
GCACTGGAGA TCAAGCTGGC CCCAGCTGGG ACAAAGCAGC ATCAGTGTGA CGCGGAGTTG 
AGGAAGGAGA TTTCCGTTGT GTGGGCCAAT CTGCCCCAGA AGACTTTGGA CTTGCTGGTA 
CCACCCCATA AGCCTGATGA GATGACAGTG GGGAAGGTTT ATGCAGCTCT GATGATATTC 
GACTTCTACA AGCAGAACAA AACCACCAGA GACCAGATGC AGCAGGCTCC TGGAGGCCTC 
TCCCAGATGG GTCCTGTGTC CCTGTTCCAC CCTCTGAAGG CCACCCTGGA GCAGACACAG 
CCGGCTGTGC TCCGAGGAGC CCGGGTTTTC CTTCGACAGA AGAGTTCCAC CTCCCTCAGC 
AATGGCGGGG CCATACAAAA CCAAGAGAGT GGCATCAAAG AGTCTGTCTC CTGGGGCACT 
CAAAGGACCC AGGATGCACC CCATGAGGCC AGGCCACCCC TGGAGCGTGG CCACTCCACA 
GAGATCCCTG TGGGGCGGTC AGGAGCACTG GCTGTGGACG TTCAGATGCA GAGCATAACC 
CGGAGGGGCC CTGATGGGGA GCCCCAGCCT GGGCTGGAGA GCCAGGGTCG AGCGGCCTCC 
ATGCCCCGCC TTGCGGCCGA GACTCAGCCC GTCACAGATG CCAGCCCCAT GAAGCGCTCC 
ATCTCCACGC TGGCCCAGCG GCCCCGTGGG ACTCATCTTT GCAGCACCAC CCCGGACCGC 
CCACCCCCTA GCCAGGCGTC GTCGCACCAC CACCACCACC GCTGCCACCG CCGCAGGGAC 
AGGAAGCAGA GGTCCCTGGA GAAGGGGCCC AGCCTGTCTG CCGATATGGA TGGCGCACCA 
AGCAGTGCTG TGGGGCCGGG GCTGCCCCCG GGAGAGGGGC CTACAGGCTG CCGGCGGGAA 
CGAGAGCGCC GGCAGGAGCG GGGCCGGTCC CAGGAGCGGA GGCAGCCCTC ATCCTCCTCC 
TCGGAGAAGC AGCGCTTCTA CTCCTGCGAC CGCTTTGGGG GCCGTGAGCC CCCGAAGCCC 
AAGCCCTCCC TCAGCAGCCA CCCAACGTCG CCAACAGCTG GCCAGGAGCC GGGACCCCAC 
CCACAGGGCA GTGGTTCCGT GAATGGGAGC CCCTTGCTGT CAACATCTGG TGCTAGCACC 
CCCGGCCGCG GTGGGCGGAG GCAGCTCCCC CAGACGCCCC TGACTCCCCG CCCCAGCATC 
ACCTACAAGA CGGCCAACTC CTCACCCATC CACTTCGCCG GGGCTCAGAC CAGCCTCCCT 
GCCTTCTCCC CAGGCCGGCT CAGCCGTGGG CTTTCCGAAC ACAACGCCCT GCTGCAGAGA 
GACCCCCTCA GCCAGCCCCT GGCCCCTGGC TCTCGAATTG GCTCTGACCC TTACCTGGGG 
CAGCGTCTGG ACAGTGAGGC CTCTGTCCAC GCCCTGCCTG AGGACACTCT CACTTTCGAG 
GAGGCTGTGG CCACCAACTC GGGCCGCTCC TCCAGGACTT CCTACGTGTC CTCCCTGACC 
TCCCAGTCTC ACCCTCTCCG CCGCGTGCCC AACGGTTACC ACTGCACCCT GGGACTCAGC 
TCGGGTGGCC GAGCACGGCA CAGCTACCAC CACCCTGACC AAGACCACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_017015119.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870608.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X10, mRNA.

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CloneID	OHu98902
Clone ID Related Accession (Same CDS sequence)	XM_017015119.1
Accession Version	XM_017015119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGCAGTGCT GAAGAGAGCG 
GCCACCAAGA AGAGCAGAAA TGACCTGATC CACGCAGAGG AGGGAGAGGA CCGGTTTGCA 
GATCTCTGTG CTGTTGGATC CCCCTTCGCC CGCGCCAGCC TCAAGAGCGG GAAGACAGAG 
AGCTCGTCAT ACTTCCGGAG GAAGGAGAAG ATGTTCCGGT TTTTTATCCG GCGCATGGTG 
AAGGCTCAGA GCTTCTACTG GGTGGTGCTG TGCGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ATTACAACCA GCCGCGGCGG CTTACCACGA CCCTGTATTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGA 
AGCTACTTCC GGTCCTCCTT CAACTGCTTC GACTTTGGGG TCATCGTGGG GAGCGTCTTT 
GAAGTGGTCT GGGCGGCCAT CAAGCCGGGA AGCTCCTTTG GGATCAGTGT GCTGCGGGCC 
CTCCGCCTGC TGAGGATCTT CAAAGTCACG AAGTACTGGA GCTCCCTGCG GAACCTGGTG 
GTGTCCCTGC TGAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCTTGCT CTTCCTGTTC 
ATTGTGGTCT TCGCCCTGCT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTCCAGGAT 
GAGACTCCCA CAACCAACTT CGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC 
CTGACGGGAG AGGACTGGAA TGCAGTGATG TATCACGGGA TCGAATCGCA AGGCGGCGTC 
AGCAAAGGCA TGTTCTCGTC CTTTTACTTC ATTGTCCTGA CACTGTTCGG AAACTACACT 
CTGCTGAATG TCTTTCTGGC CATCGCTGTG GACAACCTGG CCAACGCCCA AGAGCTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA 
GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATCGCCGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTACG GCTGCAGAAC 
CTGCGGGCCA GCTGCGAGGC GCTGTACAGC GAGATGGACC CCGAGGAGCG GCTGCGCTTC 
GCCACTACGC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTGGTGGTG 
GAGCTGGGCC GCGACGGCGC GCGGGGGCCC GTGGGAGGCA AAGCCCGACC TGAGGCTGCG 
GAGGCCCCCG AGGGCGTCGA CCCTCCGCGC AGGCACCACC GGCACCGCGA CAAGGACAAG 
ACCCCCGCGG CGGGGGACCA GGACCGAGCA GAGGCCCCGA AGGCGGAGAG CGGGGAGCCC 
GGTGCCCGGG AGGAGCGGCC GCGGCCGCAC CGCAGCCACA GCAAGGAGGC CGCGGGGCCC 
CCGGAGGCGC GGAGCGAGCG CGGCCGAGGC CCAGGCCCCG AGGGCGGCCG GCGGCACCAC 
CGGCGCGGCT CCCCGGAGGA GGCGGCCGAG CGGGAGCCCC GACGCCACCG CGCGCACCGG 
CACCAGGATC CGAGCAAGGA GTGCGCCGGC GCCAAGGGCG AGCGGCGCGC GCGGCACCGC 
GGCGGCCCCC GAGCGGGGCC CCGGGAGGCG GAGAGCGGGG AGGAGCCGGC GCGGCGGCAC 
CGGGCCCGGC ACAAGGCGCA GCCTGCTCAC GAGGCTGTGG AGAAGGAGAC CACGGAGAAG 
GAGGCCACGG AGAAGGAGGC TGAGATAGTG GAAGCCGACA AGGAAAAGGA GCTCCGGAAC 
CACCAGCCCC GGGAGCCACA CTGTGACCTG GAGACCAGTG GGACTGTGAC TGTGGGTCCC 
ATGCACACAC TGCCCAGCAC CTGTCTCCAG AAGGTGGAGG AACAGCCAGA GGATGCAGAC 
AATCAGCGGA ACGTCACTCG CATGGGCAGT CAGCCCCCAG ACCCGAACAC TATTGTACAT 
ATCCCAGTGA TGCTGACGGG CCCTCTTGGG GAAGCCACGG TCGTTCCCAG TGGTAACGTG 
GACCTGGAAA GCCAAGCAGA GGGGAAGAAG GAGGTGGAAG CGGATGACGT GATGAGGAGC 
GGCCCCCGGC CTATCGTCCC ATACAGCTCC ATGTTCTGTT TAAGCCCCAC CAACCTGCTC 
CGCCGCTTCT GCCACTACAT CGTGACCATG AGGTACTTCG AGGTGGTCAT TCTCGTGGTC 
ATCGCCTTGA GCAGCATCGC CCTGGCTGCT GAGGACCCAG TGCGCACAGA CTCGCCCAGG 
AACAACGCTC TGAAATACCT GGATTACATT TTCACTGGTG TCTTTACCTT TGAGATGGTG 
ATAAAGATGA TCGACTTGGG ACTGCTGCTT CACCCTGGAG CCTATTTCCG GGACTTGTGG 
AACATTCTGG ACTTCATTGT GGTCAGTGGC GCCCTGGTGG CGTTTGCTTT CTCGAGCTTC 
GTGGGAGGAT CCAAAGGGAA AGACATCAAT ACCATCAAGT CTCTGAGAGT CCTTCGTGTC 
CTGCGGCCCC TCAAGACCAT CAAACGGCTG CCCAAGCTCA AGGCTGTGTT TGACTGTGTG 
GTGAACTCCC TGAAGAATGT CCTCAACATC TTGATTGTCT ACATGCTCTT CATGTTCATA 
TTTGCCGTCA TTGCGGTGCA GCTCTTCAAA GGGAAGTTTT TCTACTGCAC AGATGAATCC 
AAGGAGCTGG AGAGGGACTG CAGGGGTCAG TATTTGGATT ATGAGAAGGA GGAAGTGGAA 
GCTCAGCCCA GGCAGTGGAA GAAATACGAC TTTCACTACG ACAATGTGCT CTGGGCTCTG 
CTGACGCTGT TCACAGTGTC CACGGGAGAA GGCTGGCCCA TGGTGCTGAA ACACTCCGTG 
GATGCCACCT ATGAGGAGCA GGGTCCAAGC CCTGGGTACC GCATGGAGCT GTCCATCTTC 
TACGTGGTCT ACTTTGTGGT CTTTCCCTTC TTCTTCGTCA ACATCTTTGT GGCTTTGATC 
ATCATCACCT TCCAGGAGCA GGGGGACAAG GTGATGTCTG AATGCAGCCT GGAGAAGAAC 
GAGAGGGCTT GCATTGACTT CGCCATCAGC GCCAAACCCC TGACACGGTA CATGCCCCAA 
AACCGGCAGT CGTTCCAGTA TAAGACGTGG ACATTTGTGG TCTCCCCGCC CTTTGAATAC 
TTCATCATGG CCATGATAGC CCTCAACACT GTGGTGCTGA TGATGAAGTT CTATGATGCA 
CCCTATGAGT ACGAGCTGAT GCTGAAATGC CTGAACATCG TGTTCACATC CATGTTCTCC 
ATGGAATGCG TGCTGAAGAT CATCGCCTTT GGGGTGCTGA ACTATTTCAG AGATGCCTGG 
AATGTCTTTG ACTTTGTCAC TGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGAAACGA ACAATTTCAT CAACCTCAGC TTCCTCCGCC TCTTTCGAGC TGCGCGGCTG 
ATCAAGCTGC TCCGCCAGGG CTACACCATC CGCATCCTGC TGTGGACCTT TGTCCAGTCC 
TTCAAGGCCC TGCCCTACGT GTGTCTGCTC ATTGCCATGC TGTTCTTCAT CTACGCCATC 
ATCGGCATGC AGGTGTTTGG GAATATTGCC CTGGATGATG ACACCAGCAT CAACCGCCAC 
AACAACTTCC GGACGTTTTT GCAAGCCCTG ATGCTGCTGT TCAGGAGCGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCCTGCCTG AGCAACCAGG CCTGTGATGA GCAGGCCAAT 
GCCACCGAGT GTGGAAGTGA CTTTGCCTAC TTCTACTTCG TCTCCTTCAT CTTCCTGTGC 
TCCTTTCTGA TGTTGAACCT CTTTGTGGCT GTGATCATGG ACAATTTTGA GTACCTCACG 
CGGGACTCTT CCATCCTAGG TCCTCACCAC TTGGATGAGT TCATCCGGGT CTGGGCTGAA 
TACGACCCGG CTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870608.1
CDS	109..5346
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDAVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSSFVGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAA

Target ORF information:

RefSeq Version	XM_017015119.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015119.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGCAGTGCT GAAGAGAGCG 
GCCACCAAGA AGAGCAGAAA TGACCTGATC CACGCAGAGG AGGGAGAGGA CCGGTTTGCA 
GATCTCTGTG CTGTTGGATC CCCCTTCGCC CGCGCCAGCC TCAAGAGCGG GAAGACAGAG 
AGCTCGTCAT ACTTCCGGAG GAAGGAGAAG ATGTTCCGGT TTTTTATCCG GCGCATGGTG 
AAGGCTCAGA GCTTCTACTG GGTGGTGCTG TGCGTGGTGG CCCTGAACAC ACTGTGTGTG 
GCCATGGTGC ATTACAACCA GCCGCGGCGG CTTACCACGA CCCTGTATTT TGCAGAGTTT 
GTTTTCCTGG GTCTCTTCCT CACAGAGATG TCCCTGAAGA TGTATGGCCT GGGGCCCAGA 
AGCTACTTCC GGTCCTCCTT CAACTGCTTC GACTTTGGGG TCATCGTGGG GAGCGTCTTT 
GAAGTGGTCT GGGCGGCCAT CAAGCCGGGA AGCTCCTTTG GGATCAGTGT GCTGCGGGCC 
CTCCGCCTGC TGAGGATCTT CAAAGTCACG AAGTACTGGA GCTCCCTGCG GAACCTGGTG 
GTGTCCCTGC TGAACTCCAT GAAGTCCATC ATCAGCCTGC TCTTCTTGCT CTTCCTGTTC 
ATTGTGGTCT TCGCCCTGCT GGGGATGCAG CTGTTTGGGG GACAGTTCAA CTTCCAGGAT 
GAGACTCCCA CAACCAACTT CGACACCTTC CCTGCCGCCA TCCTCACTGT CTTCCAGATC 
CTGACGGGAG AGGACTGGAA TGCAGTGATG TATCACGGGA TCGAATCGCA AGGCGGCGTC 
AGCAAAGGCA TGTTCTCGTC CTTTTACTTC ATTGTCCTGA CACTGTTCGG AAACTACACT 
CTGCTGAATG TCTTTCTGGC CATCGCTGTG GACAACCTGG CCAACGCCCA AGAGCTGACC 
AAGGATGAAG AGGAGATGGA AGAAGCAGCC AATCAGAAGC TTGCTCTGCA AAAGGCCAAA 
GAAGTGGCTG AAGTCAGCCC CATGTCTGCC GCGAACATCT CCATCGCCGC CAGGCAGCAG 
AACTCGGCCA AGGCGCGCTC GGTGTGGGAG CAGCGGGCCA GCCAGCTACG GCTGCAGAAC 
CTGCGGGCCA GCTGCGAGGC GCTGTACAGC GAGATGGACC CCGAGGAGCG GCTGCGCTTC 
GCCACTACGC GCCACCTGCG GCCCGACATG AAGACGCACC TGGACCGGCC GCTGGTGGTG 
GAGCTGGGCC GCGACGGCGC GCGGGGGCCC GTGGGAGGCA AAGCCCGACC TGAGGCTGCG 
GAGGCCCCCG AGGGCGTCGA CCCTCCGCGC AGGCACCACC GGCACCGCGA CAAGGACAAG 
ACCCCCGCGG CGGGGGACCA GGACCGAGCA GAGGCCCCGA AGGCGGAGAG CGGGGAGCCC 
GGTGCCCGGG AGGAGCGGCC GCGGCCGCAC CGCAGCCACA GCAAGGAGGC CGCGGGGCCC 
CCGGAGGCGC GGAGCGAGCG CGGCCGAGGC CCAGGCCCCG AGGGCGGCCG GCGGCACCAC 
CGGCGCGGCT CCCCGGAGGA GGCGGCCGAG CGGGAGCCCC GACGCCACCG CGCGCACCGG 
CACCAGGATC CGAGCAAGGA GTGCGCCGGC GCCAAGGGCG AGCGGCGCGC GCGGCACCGC 
GGCGGCCCCC GAGCGGGGCC CCGGGAGGCG GAGAGCGGGG AGGAGCCGGC GCGGCGGCAC 
CGGGCCCGGC ACAAGGCGCA GCCTGCTCAC GAGGCTGTGG AGAAGGAGAC CACGGAGAAG 
GAGGCCACGG AGAAGGAGGC TGAGATAGTG GAAGCCGACA AGGAAAAGGA GCTCCGGAAC 
CACCAGCCCC GGGAGCCACA CTGTGACCTG GAGACCAGTG GGACTGTGAC TGTGGGTCCC 
ATGCACACAC TGCCCAGCAC CTGTCTCCAG AAGGTGGAGG AACAGCCAGA GGATGCAGAC 
AATCAGCGGA ACGTCACTCG CATGGGCAGT CAGCCCCCAG ACCCGAACAC TATTGTACAT 
ATCCCAGTGA TGCTGACGGG CCCTCTTGGG GAAGCCACGG TCGTTCCCAG TGGTAACGTG 
GACCTGGAAA GCCAAGCAGA GGGGAAGAAG GAGGTGGAAG CGGATGACGT GATGAGGAGC 
GGCCCCCGGC CTATCGTCCC ATACAGCTCC ATGTTCTGTT TAAGCCCCAC CAACCTGCTC 
CGCCGCTTCT GCCACTACAT CGTGACCATG AGGTACTTCG AGGTGGTCAT TCTCGTGGTC 
ATCGCCTTGA GCAGCATCGC CCTGGCTGCT GAGGACCCAG TGCGCACAGA CTCGCCCAGG 
AACAACGCTC TGAAATACCT GGATTACATT TTCACTGGTG TCTTTACCTT TGAGATGGTG 
ATAAAGATGA TCGACTTGGG ACTGCTGCTT CACCCTGGAG CCTATTTCCG GGACTTGTGG 
AACATTCTGG ACTTCATTGT GGTCAGTGGC GCCCTGGTGG CGTTTGCTTT CTCGAGCTTC 
GTGGGAGGAT CCAAAGGGAA AGACATCAAT ACCATCAAGT CTCTGAGAGT CCTTCGTGTC 
CTGCGGCCCC TCAAGACCAT CAAACGGCTG CCCAAGCTCA AGGCTGTGTT TGACTGTGTG 
GTGAACTCCC TGAAGAATGT CCTCAACATC TTGATTGTCT ACATGCTCTT CATGTTCATA 
TTTGCCGTCA TTGCGGTGCA GCTCTTCAAA GGGAAGTTTT TCTACTGCAC AGATGAATCC 
AAGGAGCTGG AGAGGGACTG CAGGGGTCAG TATTTGGATT ATGAGAAGGA GGAAGTGGAA 
GCTCAGCCCA GGCAGTGGAA GAAATACGAC TTTCACTACG ACAATGTGCT CTGGGCTCTG 
CTGACGCTGT TCACAGTGTC CACGGGAGAA GGCTGGCCCA TGGTGCTGAA ACACTCCGTG 
GATGCCACCT ATGAGGAGCA GGGTCCAAGC CCTGGGTACC GCATGGAGCT GTCCATCTTC 
TACGTGGTCT ACTTTGTGGT CTTTCCCTTC TTCTTCGTCA ACATCTTTGT GGCTTTGATC 
ATCATCACCT TCCAGGAGCA GGGGGACAAG GTGATGTCTG AATGCAGCCT GGAGAAGAAC 
GAGAGGGCTT GCATTGACTT CGCCATCAGC GCCAAACCCC TGACACGGTA CATGCCCCAA 
AACCGGCAGT CGTTCCAGTA TAAGACGTGG ACATTTGTGG TCTCCCCGCC CTTTGAATAC 
TTCATCATGG CCATGATAGC CCTCAACACT GTGGTGCTGA TGATGAAGTT CTATGATGCA 
CCCTATGAGT ACGAGCTGAT GCTGAAATGC CTGAACATCG TGTTCACATC CATGTTCTCC 
ATGGAATGCG TGCTGAAGAT CATCGCCTTT GGGGTGCTGA ACTATTTCAG AGATGCCTGG 
AATGTCTTTG ACTTTGTCAC TGTGTTGGGA AGTATTACTG ATATTTTAGT AACAGAGATT 
GCGGAAACGA ACAATTTCAT CAACCTCAGC TTCCTCCGCC TCTTTCGAGC TGCGCGGCTG 
ATCAAGCTGC TCCGCCAGGG CTACACCATC CGCATCCTGC TGTGGACCTT TGTCCAGTCC 
TTCAAGGCCC TGCCCTACGT GTGTCTGCTC ATTGCCATGC TGTTCTTCAT CTACGCCATC 
ATCGGCATGC AGGTGTTTGG GAATATTGCC CTGGATGATG ACACCAGCAT CAACCGCCAC 
AACAACTTCC GGACGTTTTT GCAAGCCCTG ATGCTGCTGT TCAGGAGCGC CACGGGGGAG 
GCCTGGCACG AGATCATGCT GTCCTGCCTG AGCAACCAGG CCTGTGATGA GCAGGCCAAT 
GCCACCGAGT GTGGAAGTGA CTTTGCCTAC TTCTACTTCG TCTCCTTCAT CTTCCTGTGC 
TCCTTTCTGA TGTTGAACCT CTTTGTGGCT GTGATCATGG ACAATTTTGA GTACCTCACG 
CGGGACTCTT CCATCCTAGG TCCTCACCAC TTGGATGAGT TCATCCGGGT CTGGGCTGAA 
TACGACCCGG CTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1B ( XM_017015120.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870609.1 Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu98903
Clone ID Related Accession (Same CDS sequence)	XM_017015120.1
Accession Version	XM_017015120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC AGGATCCAAA 
GGGAAAGACA TCAATACCAT CAAGTCTCTG AGAGTCCTTC GTGTCCTGCG GCCCCTCAAG 
ACCATCAAAC GGCTGCCCAA GCTCAAGGCT GTGTTTGACT GTGTGGTGAA CTCCCTGAAG 
AATGTCCTCA ACATCTTGAT TGTCTACATG CTCTTCATGT TCATATTTGC CGTCATTGCG 
GTGCAGCTCT TCAAAGGGAA GTTTTTCTAC TGCACAGATG AATCCAAGGA GCTGGAGAGG 
GACTGCAGGG GTCAGTATTT GGATTATGAG AAGGAGGAAG TGGAAGCTCA GCCCAGGCAG 
TGGAAGAAAT ACGACTTTCA CTACGACAAT GTGCTCTGGG CTCTGCTGAC GCTGTTCACA 
GTGTCCACGG GAGAAGGCTG GCCCATGGTG CTGAAACACT CCGTGGATGC CACCTATGAG 
GAGCAGGGTC CAAGCCCTGG GTACCGCATG GAGCTGTCCA TCTTCTACGT GGTCTACTTT 
GTGGTCTTTC CCTTCTTCTT CGTCAACATC TTTGTGGCTT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGGTGAT GTCTGAATGC AGCCTGGAGA AGAACGAGAG GGCTTGCATT 
GACTTCGCCA TCAGCGCCAA ACCCCTGACA CGGTACATGC CCCAAAACCG GCAGTCGTTC 
CAGTATAAGA CGTGGACATT TGTGGTCTCC CCGCCCTTTG AATACTTCAT CATGGCCATG 
ATAGCCCTCA ACACTGTGGT GCTGATGATG AAGTTCTATG ATGCACCCTA TGAGTACGAG 
CTGATGCTGA AATGCCTGAA CATCGTGTTC ACATCCATGT TCTCCATGGA ATGCGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAACTAT TTCAGAGATG CCTGGAATGT CTTTGACTTT 
GTCACTGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGAA CAATTTCATC 
AACCTCAGCT TCCTCCGCCT CTTTCGAGCT GCGCGGCTGA TCAAGCTGCT CCGCCAGGGC 
TACACCATCC GCATCCTGCT GTGGACCTTT GTCCAGTCCT TCAAGGCCCT GCCCTACGTG 
TGTCTGCTCA TTGCCATGCT GTTCTTCATC TACGCCATCA TCGGCATGCA GGTGTTTGGG 
AATATTGCCC TGGATGATGA CACCAGCATC AACCGCCACA ACAACTTCCG GACGTTTTTG 
CAAGCCCTGA TGCTGCTGTT CAGGAGCGCC ACGGGGGAGG CCTGGCACGA GATCATGCTG 
TCCTGCCTGA GCAACCAGGC CTGTGATGAG CAGGCCAATG CCACCGAGTG TGGAAGTGAC 
TTTGCCTACT TCTACTTCGT CTCCTTCATC TTCCTGTGCT CCTTTCTGAT GTTGAACCTC 
TTTGTGGCTG TGATCATGGA CAATTTTGAG TACCTCACGC GGGACTCTTC CATCCTAGGT 
CCTCACCACT TGGATGAGTT CATCCGGGTC TGGGCTGAAT ACGACCCGGC TGCGTGTGGG 
CGCATCAGTT ACAATGACAT GTTTGAGATG CTGAAACACA TGTCCCCGCC TCTGGGGCTG 
GGGAAGAAAT GCCCTGCTCG AGTTGCTTAC AAGCGCCTGG TTCGCATGAA CATGCCCATC 
TCCAACGAGG ACATGACTGT TCACTTCACG TCCACGCTGA TGGCCCTCAT CCGGACGGCA 
CTGGAGATCA AGCTGGCCCC AGCTGGGACA AAGCAGCATC AGTGTGACGC GGAGTTGAGG 
AAGGAGATTT CCGTTGTGTG GGCCAATCTG CCCCAGAAGA CTTTGGACTT GCTGGTACCA 
CCCCATAAGC CTGATGAGAT GACAGTGGGG AAGGTTTATG CAGCTCTGAT GATATTCGAC 
TTCTACAAGC AGAACAAAAC CACCAGAGAC CAGATGCAGC AGGCTCCTGG AGGCCTCTCC 
CAGATGGGTC CTGTGTCCCT GTTCCACCCT CTGAAGGCCA CCCTGGAGCA GACACAGCCG 
GCTGTGCTCC GAGGAGCCCG GGTTTTCCTT CGACAGAAGA GTTCCACCTC CCTCAGCAAT 
GGCGGGGCCA TACAAAACCA AGAGAGTGGC ATCAAAGAGT CTGTCTCCTG GGGCACTCAA 
AGGACCCAGG ATGCACCCCA TGAGGCCAGG CCACCCCTGG AGCGTGGCCA CTCCACAGAG 
ATCCCTGTGG GGCGGTCAGG AGCACTGGCT GTGGACGTTC AGATGCAGAG CATAACCCGG 
AGGGGCCCTG ATGGGGAGCC CCAGCCTGGG CTGGAGAGCC AGGGTCGAGC GGCCTCCATG 
CCCCGCCTTG CGGCCGAGAC TCAGCCCGTC ACAGATGCCA GCCCCATGAA GCGCTCCATC 
TCCACGCTGG CCCAGCGGCC CCGTGGGACT CATCTTTGCA GCACCACCCC GGACCGCCCA 
CCCCCTAGCC AGGCGTCGTC GCACCACCAC CACCACCGCT GCCACCGCCG CAGGGACAGG 
AAGCAGAGGT CCCTGGAGAA GGGGCCCAGC CTGTCTGCCG ATATGGATGG CGCACCAAGC 
AGTGCTGTGG GGCCGGGGCT GCCCCCGGGA GAGGGGCCTA CAGGCTGCCG GCGGGAACGA 
GAGCGCCGGC AGGAGCGGGG CCGGTCCCAG GAGCGGAGGC AGCCCTCATC CTCCTCCTCG 
GAGAAGCAGC GCTTCTACTC CTGCGACCGC TTTGGGGGCC GTGAGCCCCC GAAGCCCAAG 
CCCTCCCTCA GCAGCCACCC AACGTCGCCA ACAGCTGGCC AGGAGCCGGG ACCCCACCCA 
CAGGGCAGTG GTTCCGTGAA TGGGAGCCCC TTGCTGTCAA CATCTGGTGC TAGCACCCCC 
GGCCGCGGTG GGCGGAGGCA GCTCCCCCAG ACGCCCCTGA CTCCCCGCCC CAGCATCACC 
TACAAGACGG CCAACTCCTC ACCCATCCAC TTCGCCGGGG CTCAGACCAG CCTCCCTGCC 
TTCTCCCCAG GCCGGCTCAG CCGTGGGCTT TCCGAACACA ACGCCCTGCT GCAGAGAGAC 
CCCCTCAGCC AGCCCCTGGC CCCTGGCTCT CGAATTGGCT CTGACCCTTA CCTGGGGCAG 
CGTCTGGACA GTGAGGCCTC TGTCCACGCC CTGCCTGAGG ACACTCTCAC TTTCGAGGAG 
GCTGTGGCCA CCAACTCGGG CCGCTCCTCC AGGACTTCCT ACGTGTCCTC CCTGACCTCC 
CAGTCTCACC CTCTCCGCCG CGTGCCCAAC GGTTACCACT GCACCCTGGG ACTCAGCTCG 
GGTGGCCGAG CACGGCACAG CTACCACCAC CCTGACCAAG ACCACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870609.1
CDS	109..7122
Translation	MVRFGDELGGRYGGPGGGERARGGGAGGAGGPGPGGLQPGQRVLYKQSIAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMDFVVVLTGILATAGTDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYLEWIFKAEEVMLAEEDRNAEEKSPLDVLKRAATKKSRNDLIHAEEGEDRFADLCAVGSPFARASLKSGKTESSSYFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNTLCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSPMSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALYSEMDPEERLRFATTRHLRPDMKTHLDRPLVVELGRDGARGPVGGKARPEAAEAPEGVDPPRRHHRHRDKDKTPAAGDQDRAEAPKAESGEPGAREERPRPHRSHSKEAAGPPEARSERGRGPGPEGGRRHHRRGSPEEAAEREPRRHRAHRHQDPSKECAGAKGERRARHRGGPRAGPREAESGEEPARRHRARHKAQPAHEAVEKETTEKEATEKEAEIVEADKEKELRNHQPREPHCDLETSGTVTVGPMHTLPSTCLQKVEEQPEDADNQRNVTRMGSQPPDPNTIVHIPVMLTGPLGEATVVPSGNVDLESQAEGKKEVEADDVMRSGPRPIVPYSSMFCLSPTNLLRRFCHYIVTMRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYCTDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTVSTGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQYKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIANNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIMLSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAGTKQHQCDAELRKEISVVWANLPQKTLDLLVPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLESQGRAASMPRLAAETQPVTDASPMKRSISTLAQRPRGTHLCSTTPDRPPPSQASSHHHHHRCHRRRDRKQRSLEKGPSLSADMDGAPSSAVGPGLPPGEGPTGCRRERERRQERGRSQERRQPSSSSSEKQRFYSCDRFGGREPPKPKPSLSSHPTSPTAGQEPGPHPQGSGSVNGSPLLSTSGASTPGRGGRRQLPQTPLTPRPSITYKTANSSPIHFAGAQTSLPAFSPGRLSRGLSEHNALLQRDPLSQPLAPGSRIGSDPYLGQRLDSEASVHALPEDTLTFEEAVATNSGRSSRTSYVSSLTSQSHPLRRVPNGYHCTLGLSSGGRARHSYHHPDQDHWC

Target ORF information:

RefSeq Version	XM_017015120.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 B (CACNA1B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015120.1

ORF Insert Sequence:

ATGGTCCGCT TCGGGGACGA GCTGGGCGGC CGCTATGGGG GCCCCGGCGG CGGAGAGCGG 
GCCCGGGGCG GCGGGGCCGG CGGGGCGGGG GGCCCGGGTC CCGGGGGGCT GCAGCCCGGC 
CAGCGGGTCC TCTACAAGCA ATCGATCGCG CAGCGCGCGC GGACCATGGC GCTGTACAAC 
CCCATCCCGG TCAAGCAGAA CTGCTTCACC GTCAACCGCT CGCTCTTCGT CTTCAGCGAG 
GACAACGTCG TCCGCAAATA CGCGAAGCGC ATCACCGAGT GGCCTCCATT CGAGTATATG 
ATCCTGGCCA CCATCATCGC CAACTGCATC GTGCTGGCCC TGGAGCAGCA CCTCCCTGAT 
GGGGACAAAA CGCCCATGTC CGAGCGGCTG GACGACACGG AGCCCTATTT CATCGGGATC 
TTTTGCTTCG AGGCAGGGAT CAAAATCATC GCTCTGGGCT TTGTCTTCCA CAAGGGCTCT 
TACCTGCGGA ACGGCTGGAA CGTCATGGAC TTCGTGGTCG TCCTCACAGG GATCCTTGCC 
ACGGCTGGAA CTGACTTCGA CCTGCGAACA CTGAGGGCTG TGCGTGTGCT GAGGCCCCTG 
AAGCTGGTGT CTGGGATTCC AAGTTTGCAG GTGGTGCTCA AGTCCATCAT GAAGGCCATG 
GTTCCACTCC TGCAGATTGG GCTGCTTCTC TTCTTTGCCA TCCTCATGTT TGCCATCATT 
GGCCTGGAGT TCTACATGGG CAAGTTCCAC AAGGCCTGTT TCCCCAACAG CACAGATGCG 
GAGCCCGTGG GTGACTTCCC CTGTGGCAAG GAGGCCCCAG CCCGGCTGTG CGAGGGCGAC 
ACTGAGTGCC GGGAGTACTG GCCAGGACCC AACTTTGGCA TCACCAACTT TGACAATATC 
CTGTTTGCCA TCTTGACGGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC TGACATCCTC 
TATAATACAA ACGATGCGGC CGGCAACACC TGGAACTGGC TCTACTTCAT CCCTCTCATC 
ATCATCGGCT CCTTCTTCAT GCTCAACCTG GTGCTGGGCG TGCTCTCGGG GGAGTTTGCC 
AAGGAGCGAG AGAGGGTGGA GAACCGCCGC GCCTTCCTGA AGCTGCGCCG GCAGCAGCAG 
ATCGAGCGAG AGCTCAACGG GTACCTGGAG TGGATCTTCA AGGCGGAGGA AGTCATGCTG 
GCCGAGGAGG ACAGGAATGC AGAGGAGAAG TCCCCTTTGG ACGTGCTGAA GAGAGCGGCC 
ACCAAGAAGA GCAGAAATGA CCTGATCCAC GCAGAGGAGG GAGAGGACCG GTTTGCAGAT 
CTCTGTGCTG TTGGATCCCC CTTCGCCCGC GCCAGCCTCA AGAGCGGGAA GACAGAGAGC 
TCGTCATACT TCCGGAGGAA GGAGAAGATG TTCCGGTTTT TTATCCGGCG CATGGTGAAG 
GCTCAGAGCT TCTACTGGGT GGTGCTGTGC GTGGTGGCCC TGAACACACT GTGTGTGGCC 
ATGGTGCATT ACAACCAGCC GCGGCGGCTT ACCACGACCC TGTATTTTGC AGAGTTTGTT 
TTCCTGGGTC TCTTCCTCAC AGAGATGTCC CTGAAGATGT ATGGCCTGGG GCCCAGAAGC 
TACTTCCGGT CCTCCTTCAA CTGCTTCGAC TTTGGGGTCA TCGTGGGGAG CGTCTTTGAA 
GTGGTCTGGG CGGCCATCAA GCCGGGAAGC TCCTTTGGGA TCAGTGTGCT GCGGGCCCTC 
CGCCTGCTGA GGATCTTCAA AGTCACGAAG TACTGGAGCT CCCTGCGGAA CCTGGTGGTG 
TCCCTGCTGA ACTCCATGAA GTCCATCATC AGCCTGCTCT TCTTGCTCTT CCTGTTCATT 
GTGGTCTTCG CCCTGCTGGG GATGCAGCTG TTTGGGGGAC AGTTCAACTT CCAGGATGAG 
ACTCCCACAA CCAACTTCGA CACCTTCCCT GCCGCCATCC TCACTGTCTT CCAGATCCTG 
ACGGGAGAGG ACTGGAATGC AGTGATGTAT CACGGGATCG AATCGCAAGG CGGCGTCAGC 
AAAGGCATGT TCTCGTCCTT TTACTTCATT GTCCTGACAC TGTTCGGAAA CTACACTCTG 
CTGAATGTCT TTCTGGCCAT CGCTGTGGAC AACCTGGCCA ACGCCCAAGA GCTGACCAAG 
GATGAAGAGG AGATGGAAGA AGCAGCCAAT CAGAAGCTTG CTCTGCAAAA GGCCAAAGAA 
GTGGCTGAAG TCAGCCCCAT GTCTGCCGCG AACATCTCCA TCGCCGCCAG GCAGCAGAAC 
TCGGCCAAGG CGCGCTCGGT GTGGGAGCAG CGGGCCAGCC AGCTACGGCT GCAGAACCTG 
CGGGCCAGCT GCGAGGCGCT GTACAGCGAG ATGGACCCCG AGGAGCGGCT GCGCTTCGCC 
ACTACGCGCC ACCTGCGGCC CGACATGAAG ACGCACCTGG ACCGGCCGCT GGTGGTGGAG 
CTGGGCCGCG ACGGCGCGCG GGGGCCCGTG GGAGGCAAAG CCCGACCTGA GGCTGCGGAG 
GCCCCCGAGG GCGTCGACCC TCCGCGCAGG CACCACCGGC ACCGCGACAA GGACAAGACC 
CCCGCGGCGG GGGACCAGGA CCGAGCAGAG GCCCCGAAGG CGGAGAGCGG GGAGCCCGGT 
GCCCGGGAGG AGCGGCCGCG GCCGCACCGC AGCCACAGCA AGGAGGCCGC GGGGCCCCCG 
GAGGCGCGGA GCGAGCGCGG CCGAGGCCCA GGCCCCGAGG GCGGCCGGCG GCACCACCGG 
CGCGGCTCCC CGGAGGAGGC GGCCGAGCGG GAGCCCCGAC GCCACCGCGC GCACCGGCAC 
CAGGATCCGA GCAAGGAGTG CGCCGGCGCC AAGGGCGAGC GGCGCGCGCG GCACCGCGGC 
GGCCCCCGAG CGGGGCCCCG GGAGGCGGAG AGCGGGGAGG AGCCGGCGCG GCGGCACCGG 
GCCCGGCACA AGGCGCAGCC TGCTCACGAG GCTGTGGAGA AGGAGACCAC GGAGAAGGAG 
GCCACGGAGA AGGAGGCTGA GATAGTGGAA GCCGACAAGG AAAAGGAGCT CCGGAACCAC 
CAGCCCCGGG AGCCACACTG TGACCTGGAG ACCAGTGGGA CTGTGACTGT GGGTCCCATG 
CACACACTGC CCAGCACCTG TCTCCAGAAG GTGGAGGAAC AGCCAGAGGA TGCAGACAAT 
CAGCGGAACG TCACTCGCAT GGGCAGTCAG CCCCCAGACC CGAACACTAT TGTACATATC 
CCAGTGATGC TGACGGGCCC TCTTGGGGAA GCCACGGTCG TTCCCAGTGG TAACGTGGAC 
CTGGAAAGCC AAGCAGAGGG GAAGAAGGAG GTGGAAGCGG ATGACGTGAT GAGGAGCGGC 
CCCCGGCCTA TCGTCCCATA CAGCTCCATG TTCTGTTTAA GCCCCACCAA CCTGCTCCGC 
CGCTTCTGCC ACTACATCGT GACCATGAGG TACTTCGAGG TGGTCATTCT CGTGGTCATC 
GCCTTGAGCA GCATCGCCCT GGCTGCTGAG GACCCAGTGC GCACAGACTC GCCCAGGAAC 
AACGCTCTGA AATACCTGGA TTACATTTTC ACTGGTGTCT TTACCTTTGA GATGGTGATA 
AAGATGATCG ACTTGGGACT GCTGCTTCAC CCTGGAGCCT ATTTCCGGGA CTTGTGGAAC 
ATTCTGGACT TCATTGTGGT CAGTGGCGCC CTGGTGGCGT TTGCTTTCTC AGGATCCAAA 
GGGAAAGACA TCAATACCAT CAAGTCTCTG AGAGTCCTTC GTGTCCTGCG GCCCCTCAAG 
ACCATCAAAC GGCTGCCCAA GCTCAAGGCT GTGTTTGACT GTGTGGTGAA CTCCCTGAAG 
AATGTCCTCA ACATCTTGAT TGTCTACATG CTCTTCATGT TCATATTTGC CGTCATTGCG 
GTGCAGCTCT TCAAAGGGAA GTTTTTCTAC TGCACAGATG AATCCAAGGA GCTGGAGAGG 
GACTGCAGGG GTCAGTATTT GGATTATGAG AAGGAGGAAG TGGAAGCTCA GCCCAGGCAG 
TGGAAGAAAT ACGACTTTCA CTACGACAAT GTGCTCTGGG CTCTGCTGAC GCTGTTCACA 
GTGTCCACGG GAGAAGGCTG GCCCATGGTG CTGAAACACT CCGTGGATGC CACCTATGAG 
GAGCAGGGTC CAAGCCCTGG GTACCGCATG GAGCTGTCCA TCTTCTACGT GGTCTACTTT 
GTGGTCTTTC CCTTCTTCTT CGTCAACATC TTTGTGGCTT TGATCATCAT CACCTTCCAG 
GAGCAGGGGG ACAAGGTGAT GTCTGAATGC AGCCTGGAGA AGAACGAGAG GGCTTGCATT 
GACTTCGCCA TCAGCGCCAA ACCCCTGACA CGGTACATGC CCCAAAACCG GCAGTCGTTC 
CAGTATAAGA CGTGGACATT TGTGGTCTCC CCGCCCTTTG AATACTTCAT CATGGCCATG 
ATAGCCCTCA ACACTGTGGT GCTGATGATG AAGTTCTATG ATGCACCCTA TGAGTACGAG 
CTGATGCTGA AATGCCTGAA CATCGTGTTC ACATCCATGT TCTCCATGGA ATGCGTGCTG 
AAGATCATCG CCTTTGGGGT GCTGAACTAT TTCAGAGATG CCTGGAATGT CTTTGACTTT 
GTCACTGTGT TGGGAAGTAT TACTGATATT TTAGTAACAG AGATTGCGAA CAATTTCATC 
AACCTCAGCT TCCTCCGCCT CTTTCGAGCT GCGCGGCTGA TCAAGCTGCT CCGCCAGGGC 
TACACCATCC GCATCCTGCT GTGGACCTTT GTCCAGTCCT TCAAGGCCCT GCCCTACGTG 
TGTCTGCTCA TTGCCATGCT GTTCTTCATC TACGCCATCA TCGGCATGCA GGTGTTTGGG 
AATATTGCCC TGGATGATGA CACCAGCATC AACCGCCACA ACAACTTCCG GACGTTTTTG 
CAAGCCCTGA TGCTGCTGTT CAGGAGCGCC ACGGGGGAGG CCTGGCACGA GATCATGCTG 
TCCTGCCTGA GCAACCAGGC CTGTGATGAG CAGGCCAATG CCACCGAGTG TGGAAGTGAC 
TTTGCCTACT TCTACTTCGT CTCCTTCATC TTCCTGTGCT CCTTTCTGAT GTTGAACCTC 
TTTGTGGCTG TGATCATGGA CAATTTTGAG TACCTCACGC GGGACTCTTC CATCCTAGGT 
CCTCACCACT TGGATGAGTT CATCCGGGTC TGGGCTGAAT ACGACCCGGC TGCGTGTGGG 
CGCATCAGTT ACAATGACAT GTTTGAGATG CTGAAACACA TGTCCCCGCC TCTGGGGCTG 
GGGAAGAAAT GCCCTGCTCG AGTTGCTTAC AAGCGCCTGG TTCGCATGAA CATGCCCATC 
TCCAACGAGG ACATGACTGT TCACTTCACG TCCACGCTGA TGGCCCTCAT CCGGACGGCA 
CTGGAGATCA AGCTGGCCCC AGCTGGGACA AAGCAGCATC AGTGTGACGC GGAGTTGAGG 
AAGGAGATTT CCGTTGTGTG GGCCAATCTG CCCCAGAAGA CTTTGGACTT GCTGGTACCA 
CCCCATAAGC CTGATGAGAT GACAGTGGGG AAGGTTTATG CAGCTCTGAT GATATTCGAC 
TTCTACAAGC AGAACAAAAC CACCAGAGAC CAGATGCAGC AGGCTCCTGG AGGCCTCTCC 
CAGATGGGTC CTGTGTCCCT GTTCCACCCT CTGAAGGCCA CCCTGGAGCA GACACAGCCG 
GCTGTGCTCC GAGGAGCCCG GGTTTTCCTT CGACAGAAGA GTTCCACCTC CCTCAGCAAT 
GGCGGGGCCA TACAAAACCA AGAGAGTGGC ATCAAAGAGT CTGTCTCCTG GGGCACTCAA 
AGGACCCAGG ATGCACCCCA TGAGGCCAGG CCACCCCTGG AGCGTGGCCA CTCCACAGAG 
ATCCCTGTGG GGCGGTCAGG AGCACTGGCT GTGGACGTTC AGATGCAGAG CATAACCCGG 
AGGGGCCCTG ATGGGGAGCC CCAGCCTGGG CTGGAGAGCC AGGGTCGAGC GGCCTCCATG 
CCCCGCCTTG CGGCCGAGAC TCAGCCCGTC ACAGATGCCA GCCCCATGAA GCGCTCCATC 
TCCACGCTGG CCCAGCGGCC CCGTGGGACT CATCTTTGCA GCACCACCCC GGACCGCCCA 
CCCCCTAGCC AGGCGTCGTC GCACCACCAC CACCACCGCT GCCACCGCCG CAGGGACAGG 
AAGCAGAGGT CCCTGGAGAA GGGGCCCAGC CTGTCTGCCG ATATGGATGG CGCACCAAGC 
AGTGCTGTGG GGCCGGGGCT GCCCCCGGGA GAGGGGCCTA CAGGCTGCCG GCGGGAACGA 
GAGCGCCGGC AGGAGCGGGG CCGGTCCCAG GAGCGGAGGC AGCCCTCATC CTCCTCCTCG 
GAGAAGCAGC GCTTCTACTC CTGCGACCGC TTTGGGGGCC GTGAGCCCCC GAAGCCCAAG 
CCCTCCCTCA GCAGCCACCC AACGTCGCCA ACAGCTGGCC AGGAGCCGGG ACCCCACCCA 
CAGGGCAGTG GTTCCGTGAA TGGGAGCCCC TTGCTGTCAA CATCTGGTGC TAGCACCCCC 
GGCCGCGGTG GGCGGAGGCA GCTCCCCCAG ACGCCCCTGA CTCCCCGCCC CAGCATCACC 
TACAAGACGG CCAACTCCTC ACCCATCCAC TTCGCCGGGG CTCAGACCAG CCTCCCTGCC 
TTCTCCCCAG GCCGGCTCAG CCGTGGGCTT TCCGAACACA ACGCCCTGCT GCAGAGAGAC 
CCCCTCAGCC AGCCCCTGGC CCCTGGCTCT CGAATTGGCT CTGACCCTTA CCTGGGGCAG 
CGTCTGGACA GTGAGGCCTC TGTCCACGCC CTGCCTGAGG ACACTCTCAC TTTCGAGGAG 
GCTGTGGCCA CCAACTCGGG CCGCTCCTCC AGGACTTCCT ACGTGTCCTC CCTGACCTCC 
CAGTCTCACC CTCTCCGCCG CGTGCCCAAC GGTTACCACT GCACCCTGGG ACTCAGCTCG 
GGTGGCCGAG CACGGCACAG CTACCACCAC CCTGACCAAG ACCACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CACNA1B
Entrez Gene ID	774
Full Name	calcium voltage-gated channel subunit alpha1 B
Synonyms	BIII,CACNL1A5,CACNN,Cav2.2,DYT23
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 B Names voltage-dependent N-type calcium channel subunit alpha-1B Cav2.2 voltage-gated Ca2+ channel brain calcium channel III calcium channel alpha12.2 subunit calcium channel, L type, alpha-1 polypeptide calcium channel, voltage-dependent, L type, alpha 1B subunit calcium channel, voltage-dependent, N type, alpha 1B subunit voltage-gated calcium channel alpha subunit Cav2.2 voltage-gated calcium channel subunit alpha Cav2.2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.3
Summary	The protein encoded by this gene is the pore-forming subunit of an N-type voltage-dependent calcium channel, which controls neurotransmitter release from neurons. The encoded protein forms a complex with alpha-2, beta, and delta subunits to form the high-voltage activated channel. This channel is sensitive to omega-conotoxin-GVIA and omega-agatoxin-IIIA but insensitive to dihydropyridines. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011].
Disorder MIM:	601012
Disorder Html:	?Dystonia 23, 614860 (3)

Pan troglodytes (chimpanzee)	CACNA1B	XP_520396.4
Canis lupus familiaris (dog)	CACNA1B	XP_005625483.1
Rattus norvegicus (Norway rat)	Cacna1b	NP_671482.1
Danio rerio (zebrafish)	cacna1ba	NP_001108020.1
Caenorhabditis elegans (roundworm)	unc-2	NP_001123176.1
Gallus gallus (chicken)	CACNA1B	NP_989624.1
Drosophila melanogaster (fruit fly)	cac	NP_001245636.1
Homo sapiens (human)	CACNA1B	NP_000709.1
Bos taurus (cattle)	CACNA1B	NP_777057.1
Mus musculus (house mouse)	Cacna1b	NP_001035993.1
Danio rerio (zebrafish)	cacna1bb	XP_002665969.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002578	XP_312358.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CACNA1B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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