TM4SF4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TM4SF4
Entrez Gene ID	7104
Full Name	transmembrane 4 L six family member 4
Synonyms	ILTMP,il-TMP
General protein information	Preferred Names transmembrane 4 L six family member 4 Names transmembrane 4 L6 family member 4 intestinal and liver (il) tetraspan membrane protein intestine and liver tetraspan membrane protein transmembrane 4 superfamily member 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q25.1
Summary	The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that can regulate cell proliferation.[provided by RefSeq, Mar 2011].
Disorder MIM:	606567

mRNA and Protein(s)

mRNA	Protein	Name
NM_004617.4	NP_004608.1	transmembrane 4 L6 family member 4
NM_004617.3	NP_004608.1	transmembrane 4 L6 family member 4

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	tm4sf4	NP_001003489.1
Mus musculus (house mouse)	Tm4sf4	NP_663514.2
Rattus norvegicus (Norway rat)	Tm4sf4	NP_446237.1
Xenopus tropicalis (tropical clawed frog)	tm4sf4	NP_988958.1
Homo sapiens (human)	TM4SF4	NP_004608.1
	TM4SF4	XP_001108653.1
Canis lupus familiaris (dog)	TM4SF4	XP_854442.1
Gallus gallus (chicken)	TM4SF4	XP_001234023.1
Pan troglodytes (chimpanzee)	TM4SF4	XP_001170324.1
Bos taurus (cattle)	TM4SF4	NP_001029478.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
Cheong JG, Song DG, Song HE, Berditchevski F, Nam SH, Jung JW, Kim HJ, Kim JE, Kim S, Ryu J, Cho CY, Lee KM, Lee JW
Oncotarget8(8)13277-13292(2017 Feb)

TM4SF4 overexpression in radiation-resistant lung carcinoma cells activates IGF1R via elevation of IGF1.
Choi SI, Kim SY, Lee J, Cho EW, Kim IG
Oncotarget5(20)9823-37(2014 Oct)

Association of TM4SF4 with the human thiamine transporter-2 in intestinal epithelial cells.
Subramanian VS, Nabokina SM, Said HM
Digestive diseases and sciences59(3)583-90(2014 Mar)

Adenovirus-mediated delivery of siRNA targeting TM4SF4 attenuated liver cancer cell growth in vitro and in vivo.
Wang L, Feng J, Da L, Li Y, Li Z, Zhao M
Acta biochimica et biophysica Sinica45(3)213-9(2013 Mar)

Overexpression of S100B, TM4SF4, and OLFM4 genes is correlated with liver metastasis in Taiwanese colorectal cancer patients.
Huang MY, Wang HM, Chang HJ, Hsiao CP, Wang JY, Lin SR
DNA and cell biology31(1)43-9(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Replacement of the transmembrane 4 L six family protein TM4SF1 or TM4SF4 C-terminus with that of TM4SF5 increased spheroids growth, transwell migration, and invasive dissemination from spheroids in 3D collagen gels.
Title: Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.

TM4SF4 expression was correlated with the increased expression of IGF1, consequently resulting in IGF1R activation in lung adenocarcinoma.
Title: TM4SF4 overexpression in radiation-resistant lung carcinoma cells activates IGF1R via elevation of IGF1.

TM4SF4 interacts with hTHTR-2 and influences the physiological function of the thiamine transporter in human intestinal epithelial cells.
Title: Association of TM4SF4 with the human thiamine transporter-2 in intestinal epithelial cells.

TM4SF4 is a membrane protein that may have a role in attenuating liver cancer cell growth
Title: Adenovirus-mediated delivery of siRNA targeting TM4SF4 attenuated liver cancer cell growth in vitro and in vivo.

Results suggest that S100B, TM4SF3, and OLFM4 overexpression may affect metastatic behavior of tumor cells in Taiwanese colorectal cancer patients.
Title: Overexpression of S100B, TM4SF4, and OLFM4 genes is correlated with liver metastasis in Taiwanese colorectal cancer patients.

The following TM4SF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TM4SF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16935	NM_004617.4 Latest version!	Homo sapiens transmembrane 4 L six family member 4 (TM4SF4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu16935	NM_004617.3	Homo sapiens transmembrane 4 L six family member 4 (TM4SF4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TM4SF4 ( NM_004617.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004608.1 Homo sapiens transmembrane 4 L six family member 4 (TM4SF4), mRNA.

Price & Availability↑

CloneID	OHu16935
Clone ID Related Accession (Same CDS sequence)	NM_004617.4 , NM_004617.3
Accession Version	NM_004617.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	transmembrane 4 L6 family member 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP294159.1, U31449.1 and BG656032.1. On May 17, 2019 this sequence version replaced NM_004617.3. ##Evidence-Data-START## Transcript exon combination :: SRR5189664.75216.1, SRR5189664.128237.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000305354.5/ ENSP00000305852.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGCACTG GGGGCTGTGC CAGATGCCTG GGGGGGACCC TCATTCCCCT TGCTTTTTTT 
GGCTTCCTGG CTAACATCCT GTTATTTTTT CCTGGAGGAA AAGTGATAGA TGACAACGAC 
CACCTTTCCC AAGAGATCTG GTTTTTCGGA GGAATATTAG GAAGCGGTGT CTTGATGATC 
TTCCCTGCGC TGGTGTTCTT GGGCCTGAAG AACAATGACT GCTGTGGGTG CTGCGGCAAC 
GAGGGCTGTG GGAAGCGATT TGCGATGTTC ACCTCCACGA TATTTGCTGT GGTTGGATTC 
TTGGGAGCTG GATACTCGTT TATCATCTCA GCCATTTCAA TCAACAAGGG TCCTAAATGC 
CTCATGGCCA ATAGTACATG GGGCTACCCC TTCCACGACG GGGATTATCT CAATGATGAG 
GCCTTATGGA ACAAGTGCCG AGAGCCTCTC AATGTGGTTC CCTGGAATCT GACCCTCTTC 
TCCATCCTGC TGGTCGTAGG AGGAATCCAG ATGGTTCTCT GCGCCATCCA GGTGGTCAAT 
GGCCTCCTGG GGACCCTCTG TGGGGACTGC CAGTGTTGTG GCTGCTGTGG GGGAGATGGA 
CCCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004608.1
CDS	182..790
Translation	MCTGGCARCLGGTLIPLAFFGFLANILLFFPGGKVIDDNDHLSQEIWFFGGILGSGVLMIFPALVFLGLKNNDCCGCCGNEGCGKRFAMFTSTIFAVVGFLGAGYSFIISAISINKGPKCLMANSTWGYPFHDGDYLNDEALWNKCREPLNVVPWNLTLFSILLVVGGIQMVLCAIQVVNGLLGTLCGDCQCCGCCGGDGPV

Target ORF information:

RefSeq Version	NM_004617.4
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane 4 L six family member 4 (TM4SF4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004617.4

ORF Insert Sequence:

ATGTGCACTG GGGGCTGTGC CAGATGCCTG GGGGGGACCC TCATTCCCCT TGCTTTTTTT 
GGCTTCCTGG CTAACATCCT GTTATTTTTT CCTGGAGGAA AAGTGATAGA TGACAACGAC 
CACCTTTCCC AAGAGATCTG GTTTTTCGGA GGAATATTAG GAAGCGGTGT CTTGATGATC 
TTCCCTGCGC TGGTGTTCTT GGGCCTGAAG AACAATGACT GCTGTGGGTG CTGCGGCAAC 
GAGGGCTGTG GGAAGCGATT TGCGATGTTC ACCTCCACGA TATTTGCTGT GGTTGGATTC 
TTGGGAGCTG GATACTCGTT TATCATCTCA GCCATTTCAA TCAACAAGGG TCCTAAATGC 
CTCATGGCCA ATAGTACATG GGGCTACCCC TTCCACGACG GGGATTATCT CAATGATGAG 
GCCTTATGGA ACAAGTGCCG AGAGCCTCTC AATGTGGTTC CCTGGAATCT GACCCTCTTC 
TCCATCCTGC TGGTCGTAGG AGGAATCCAG ATGGTTCTCT GCGCCATCCA GGTGGTCAAT 
GGCCTCCTGG GGACCCTCTG TGGGGACTGC CAGTGTTGTG GCTGCTGTGG GGGAGATGGA 
CCCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TM4SF4 ( NM_004617.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004608.1 Homo sapiens transmembrane 4 L six family member 4 (TM4SF4), mRNA.

Price & Availability↑

CloneID	OHu16935
Clone ID Related Accession (Same CDS sequence)	NM_004617.4 , NM_004617.3
Accession Version	NM_004617.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	transmembrane 4 L6 family member 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP294159.1, U31449.1 and BG656032.1. On Mar 12, 2011 this sequence version replaced NM_004617.2. ##Evidence-Data-START## Transcript exon combination :: AK055577.1, ERR279860.2066.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCACTG GGGGCTGTGC CAGATGCCTG GGGGGGACCC TCATTCCCCT TGCTTTTTTT 
GGCTTCCTGG CTAACATCCT GTTATTTTTT CCTGGAGGAA AAGTGATAGA TGACAACGAC 
CACCTTTCCC AAGAGATCTG GTTTTTCGGA GGAATATTAG GAAGCGGTGT CTTGATGATC 
TTCCCTGCGC TGGTGTTCTT GGGCCTGAAG AACAATGACT GCTGTGGGTG CTGCGGCAAC 
GAGGGCTGTG GGAAGCGATT TGCGATGTTC ACCTCCACGA TATTTGCTGT GGTTGGATTC 
TTGGGAGCTG GATACTCGTT TATCATCTCA GCCATTTCAA TCAACAAGGG TCCTAAATGC 
CTCATGGCCA ATAGTACATG GGGCTACCCC TTCCACGACG GGGATTATCT CAATGATGAG 
GCCTTATGGA ACAAGTGCCG AGAGCCTCTC AATGTGGTTC CCTGGAATCT GACCCTCTTC 
TCCATCCTGC TGGTCGTAGG AGGAATCCAG ATGGTTCTCT GCGCCATCCA GGTGGTCAAT 
GGCCTCCTGG GGACCCTCTG TGGGGACTGC CAGTGTTGTG GCTGCTGTGG GGGAGATGGA 
CCCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004608.1
CDS	298..906
Translation	MCTGGCARCLGGTLIPLAFFGFLANILLFFPGGKVIDDNDHLSQEIWFFGGILGSGVLMIFPALVFLGLKNNDCCGCCGNEGCGKRFAMFTSTIFAVVGFLGAGYSFIISAISINKGPKCLMANSTWGYPFHDGDYLNDEALWNKCREPLNVVPWNLTLFSILLVVGGIQMVLCAIQVVNGLLGTLCGDCQCCGCCGGDGPV

Target ORF information:

RefSeq Version	NM_004617.3
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane 4 L six family member 4 (TM4SF4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004617.3

ORF Insert Sequence:

ATGTGCACTG GGGGCTGTGC CAGATGCCTG GGGGGGACCC TCATTCCCCT TGCTTTTTTT 
GGCTTCCTGG CTAACATCCT GTTATTTTTT CCTGGAGGAA AAGTGATAGA TGACAACGAC 
CACCTTTCCC AAGAGATCTG GTTTTTCGGA GGAATATTAG GAAGCGGTGT CTTGATGATC 
TTCCCTGCGC TGGTGTTCTT GGGCCTGAAG AACAATGACT GCTGTGGGTG CTGCGGCAAC 
GAGGGCTGTG GGAAGCGATT TGCGATGTTC ACCTCCACGA TATTTGCTGT GGTTGGATTC 
TTGGGAGCTG GATACTCGTT TATCATCTCA GCCATTTCAA TCAACAAGGG TCCTAAATGC 
CTCATGGCCA ATAGTACATG GGGCTACCCC TTCCACGACG GGGATTATCT CAATGATGAG 
GCCTTATGGA ACAAGTGCCG AGAGCCTCTC AATGTGGTTC CCTGGAATCT GACCCTCTTC 
TCCATCCTGC TGGTCGTAGG AGGAATCCAG ATGGTTCTCT GCGCCATCCA GGTGGTCAAT 
GGCCTCCTGG GGACCCTCTG TGGGGACTGC CAGTGTTGTG GCTGCTGTGG GGGAGATGGA 
CCCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential regulation of cellular functions by the C-termini of transmembrane 4 L six family proteins in 2- or 3-dimensional environment.
Oncotarget8(8)13277-13292(2017 Feb)
Cheong JG,Song DG,Song HE,Berditchevski F,Nam SH,Jung JW,Kim HJ,Kim JE,Kim S,Ryu J,Cho CY,Lee KM,Lee JW

TM4SF4 overexpression in radiation-resistant lung carcinoma cells activates IGF1R via elevation of IGF1.
Oncotarget5(20)9823-37(2014 Oct)
Choi SI,Kim SY,Lee J,Cho EW,Kim IG

Association of TM4SF4 with the human thiamine transporter-2 in intestinal epithelial cells.
Digestive diseases and sciences59(3)583-90(2014 Mar)
Subramanian VS,Nabokina SM,Said HM

Adenovirus-mediated delivery of siRNA targeting TM4SF4 attenuated liver cancer cell growth in vitro and in vivo.
Acta biochimica et biophysica Sinica45(3)213-9(2013 Mar)
Wang L,Feng J,Da L,Li Y,Li Z,Zhao M

Overexpression of S100B, TM4SF4, and OLFM4 genes is correlated with liver metastasis in Taiwanese colorectal cancer patients.
DNA and cell biology31(1)43-9(2012 Jan)
Huang MY,Wang HM,Chang HJ,Hsiao CP,Wang JY,Lin SR