TLL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLL1
Entrez Gene ID	7092
Full Name	tolloid like 1
Synonyms	ASD6,TLL
General protein information	Preferred Names tolloid like 1 Names tolloid-like protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q32.3
Summary	This gene encodes an astacin-like, zinc-dependent, metalloprotease that belongs to the peptidase M12A family. This protease processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Studies in mice suggest that this gene plays multiple roles in the development of mammalian heart, and is essential for the formation of the interventricular septum. Allelic variants of this gene are associated with atrial septal defect type 6. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011].
Disorder MIM:	606742
Disorder Html:	Atrial septal defect 6, 613087 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_012464.5	NP_036596.3	tolloid-like protein 1 isoform 1 precursor
NM_001204760.1	NP_001191689.1	tolloid-like protein 1 isoform 2 precursor
XM_024454195.1	XP_024309963.1	tolloid-like protein 1 isoform X2
NM_012464.4	NP_036596.3	tolloid-like protein 1 isoform 1 precursor
NM_001204760.2	NP_001191689.1	tolloid-like protein 1 isoform 2 precursor
XM_024454194.1	XP_024309962.1	tolloid-like protein 1 isoform X2
XM_011532214.1	XP_011530516.1	tolloid-like protein 1 isoform X3
XM_017008570.1	XP_016864059.1	tolloid-like protein 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TLL1	NP_036596.3
	TLL1	XP_001101883.2
Gallus gallus (chicken)	TLL1	NP_990034.1
Danio rerio (zebrafish)	tll1	NP_571085.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003702	XP_313494.5
Caenorhabditis elegans (roundworm)	nas-39	NP_510672.2
Drosophila melanogaster (fruit fly)	tok	NP_733035.1
Bos taurus (cattle)	TLL1	NP_001180043.1
Mus musculus (house mouse)	Tll1	NP_033416.2
Canis lupus familiaris (dog)	TLL1	XP_539791.2
Pan troglodytes (chimpanzee)	TLL1	XP_003310613.1
Rattus norvegicus (Norway rat)	Tll1	NP_001099551.1
Xenopus tropicalis (tropical clawed frog)	LOC100495742	XP_004911222.1

Gene Ontology
Bibliography

Related articles in PubMed

Combination of PNPLA3 and TLL1 polymorphism can predict advanced fibrosis in Japanese patients with nonalcoholic fatty liver disease.
Seko Y, Yamaguchi K, Mizuno N, Okuda K, Takemura M, Taketani H, Hara T, Umemura A, Nishikawa T, Moriguchi M, Yasui K, Kamaguchi M, Nishioji K, Mochizuki N, Kobayashi M, Mori K, Tanaka S, Matsuura K, Tanaka Y, Itoh Y
Journal of gastroenterology(2017 Jul)

Genome-Wide Association Study Identifies TLL1 Variant Associated With Development of Hepatocellular Carcinoma After Eradication of Hepatitis C Virus Infection.
Matsuura K, Sawai H, Ikeo K, Ogawa S, Iio E, Isogawa M, Shimada N, Komori A, Toyoda H, Kumada T, Namisaki T, Yoshiji H, Sakamoto N, Nakagawa M, Asahina Y, Kurosaki M, Izumi N, Enomoto N, Kusakabe A, Kajiwara E, Itoh Y, Ide T, Tamori A, Matsubara M, Kawada N, Shirabe K, Tomita E, Honda M, Kaneko S, Nishina S, Suetsugu A, Hiasa Y, Watanabe H, Genda T, Sakaida I, Nishiguchi S, Takaguchi K, Tanaka E, Sugihara J, Shimada M, Kondo Y, Kawai Y, Kojima K, Nagasaki M, Tokunaga K, Tanaka Y,
Gastroenterology152(6)1383-1394(2017 May)

Association of TLL1 gene polymorphism (rs1503298, T > C) with coronary heart disease in PREDICT, UDACS and ED cohorts.
Zain M, Awan FR, Cooper JA, Li KW, Palmen J, Acharya J, Howard P, Baig SM, Elkeles RS, Stephens JW, Ireland H, Humphries SE
Journal of the College of Physicians and Surgeons--Pakistan : JCPSP24(9)615-9(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Multivariate analysis showed rs17047200 AT/TT to be an independent risk factor for HCC (hazard ratio, 1.78; P = .008).
Title: Genome-Wide Association Study Identifies TLL1 Variant Associated With Development of Hepatocellular Carcinoma After Eradication of Hepatitis C Virus Infection.

This SNP [TLL1 gene ]could not be confirmed as a risk factor for CHD [coronary heart disease]in T2DM [type-2 Diabetes mellitus]patients
Title: Association of TLL1 gene polymorphism (rs1503298, T > C) with coronary heart disease in PREDICT, UDACS and ED cohorts.

This study identified TLL1 as a new susceptibility gene for PTSD.
Title: Genome-wide association study identifies new susceptibility loci for posttraumatic stress disorder.

TLL-1 gene mutation with an insertion mutation of base A in exon 10 is common in Chinese patients with sporadic congenital heart diseases.
Title: [Metalloproteinase Tolloid-like 1 gene mutation in Chinese patients with sporadic congenital heart diseases].

We identified a variant in a single PPAR pathway gene, TLL1, that is associated with the extent of coronary artery disease independently of clinical predictors, specifically in patients with type 2 diabetes mellitus.
Title: Peroxisome proliferator-activated receptor pathway gene polymorphism associated with extent of coronary artery disease in patients with type 2 diabetes in the bypass angioplasty revascularization investigation 2 diabetes trial.

The following TLL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20767	NM_012464.5 Latest version!	Homo sapiens tolloid like 1 (TLL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu17668	NM_001204760.2 Latest version!	Homo sapiens tolloid like 1 (TLL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu61040	XM_011532214.1 Latest version!	Homo sapiens tolloid like 1 (TLL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu105575	XM_024454195.1 Latest version!	Homo sapiens tolloid like 1 (TLL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu105575	XM_024454194.1 Latest version!	Homo sapiens tolloid like 1 (TLL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu95384	XM_017008570.1 Latest version!	Homo sapiens tolloid like 1 (TLL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu20767	NM_012464.4	Homo sapiens tolloid like 1 (TLL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu17668	NM_001204760.1	Homo sapiens tolloid like 1 (TLL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLL1 ( NM_012464.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_036596.3 Homo sapiens tolloid like 1 (TLL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20767
Clone ID Related Accession (Same CDS sequence)	NM_012464.5 , NM_012464.4
Accession Version	NM_012464.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tolloid-like protein 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC097502.3, U91963.1, AC097507.2, AC093874.3, BC144085.1, AF282732.1, AK021908.1 and BU753788.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_012464.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.51262.1, SRR1803617.258053.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000061240.7/ ENSP00000061240.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGTTGG GAACGCTTTC CCCGAGGATG CTCGTGTGGC TGGTGGCCTC GGGGATTGTT 
TTCTACGGGG AGCTATGGGT CTGCGCTGGC CTCGATTATG ATTACACTTT TGATGGGAAC 
GAAGAGGATA AAACAGAGAC TATAGATTAC AAGGACCCGT GTAAAGCCGC TGTATTTTGG 
GGCGATATTG CCTTAGATGA TGAAGACTTA AATATCTTTC AAATAGATAG GACAATTGAC 
CTTACGCAGA ACCCCTTTGG AAACCTTGGA CATACCACAG GTGGACTTGG AGACCATGCT 
ATGTCAAAGA AGCGAGGGGC CCTCTACCAA CTTATAGACA GGATAAGAAG AATTGGCTTT 
GGCTTGGAGC AAAACAACAC AGTTAAGGGA AAAGTACCTC TACAATTCTC AGGGCAAAAT 
GAGAAAAATC GAGTTCCCAG AGCCGCTACA TCAAGAACGG AAAGAATATG GCCTGGAGGC 
GTTATTCCTT ATGTTATAGG AGGAAACTTC ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGGC ACTGGGAAAA GCACACATGT GTGACTTTCA TAGAAAGAAG TGATGAAGAG 
AGTTACATTG TATTCACCTA TAGGCCTTGT GGATGCTGCT CCTATGTAGG TCGGCGAGGA 
AATGGACCTC AGGCAATCTC TATCGGCAAG AACTGTGATA AATTTGGGAT TGTTGTTCAT 
GAATTGGGTC ATGTGATAGG CTTTTGGCAT GAACACACAA GACCAGATCG AGATAACCAC 
GTAACTATCA TAAGAGAAAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT GAAGATGGAG 
CCTGGAGAAG TAAACTCACT TGGAGAAAGA TATGATTTCG ACAGTATCAT GCACTATGCC 
AGGAACACCT TCTCAAGGGG GATGTTTCTG GATACCATTC TCCCCTCCCG TGATGATAAT 
GGCATACGTC CTGCAATTGG TCAGCGAACC CGTCTAAGCA AAGGAGATAT CGCACAGGCA 
AGAAAGCTGT ATAGATGTCC AGCATGTGGA GAAACTCTAC AAGAATCCAA TGGCAACCTT 
TCCTCTCCAG GATTTCCCAA TGGCTACCCT TCTTACACAC ACTGCATCTG GAGAGTTTCT 
GTGACCCCAG GGGAGAAGAT TGTTTTAAAT TTTACAACGA TGGATCTATA CAAGAGTAGT 
TTGTGCTGGT ATGACTATAT TGAAGTAAGA GACGGGTACT GGAGAAAATC ACCTCTCCTT 
GGTAGATTCT GTGGGGACAA ATTGCCTGAA GTTCTTACTT CTACAGACAG CAGAATGTGG 
ATTGAGTTTC GTAGCAGCAG TAATTGGGTA GGAAAAGGCT TTGCAGCTGT CTATGAAGCG 
ATCTGTGGAG GTGAGATACG TAAAAATGAA GGACAGATTC AGTCTCCCAA TTATCCTGAT 
GACTATCGCC CGATGAAAGA ATGTGTGTGG AAAATAACAG TGTCTGAGAG CTACCACGTC 
GGGCTGACCT TTCAGTCCTT TGAGATTGAA AGACATGACA ATTGTGCTTA TGACTACCTG 
GAAGTTAGAG ATGGAACCAG TGAAAATAGC CCTTTGATAG GGCGTTTCTG TGGTTATGAC 
AAACCTGAAG ACATAAGATC TACCTCCAAT ACTTTGTGGA TGAAGTTTGT TTCTGACGGA 
ACTGTGAACA AAGCAGGGTT TGCTGCTAAC TTTTTTAAAG AGGAAGATGA GTGTGCCAAA 
CCTGACCGTG GAGGCTGTGA GCAGCGATGT CTGAACACTC TGGGCAGTTA CCAGTGTGCC 
TGTGAGCCTG GCTATGAGCT GGGCCCAGAC AGAAGGAGCT GTGAAGCTGC TTGTGGTGGA 
CTTCTTACCA AACTTAACGG CACCATAACC ACCCCTGGCT GGCCCAAGGA GTACCCTCCT 
AATAAGAACT GTGTGTGGCA AGTGGTTGCA CCAACCCAGT ACAGAATTTC TGTGAAGTTT 
GAGTTTTTTG AATTGGAAGG CAATGAAGTT TGCAAATATG ATTATGTGGA GATCTGGAGT 
GGTCTTTCCT CTGAGTCTAA ACTGCATGGC AAATTCTGTG GCGCTGAAGT GCCTGAAGTG 
ATCACATCCC AGTTCAACAA TATGAGAATT GAATTCAAAT CTGACAATAC TGTATCCAAG 
AAGGGCTTCA AAGCACATTT TTTCTCAGAC AAAGATGAAT GCTCTAAGGA TAATGGTGGA 
TGTCAGCACG AATGTGTCAA CACGATGGGG AGCTACATGT GTCAATGCCG TAATGGATTT 
GTGCTACATG ACAATAAACA TGATTGCAAG GAAGCTGAGT GTGAACAGAA GATCCACAGT 
CCAAGTGGCC TCATCACCAG TCCCAACTGG CCAGACAAGT ACCCAAGCAG GAAAGAATGC 
ACTTGGGAAA TCAGCGCCAC TCCTGGCCAC CGAATCAAAT TAGCCTTTAG TGAATTTGAG 
ATTGAGCAGC ATCAAGAATG TGCTTATGAC CACTTAGAAG TATTTGATGG AGAAACAGAA 
AAGTCACCGA TTCTTGGACG ACTGTGTGGC AACAAGATAC CAGATCCCCT TGTGGCTACT 
GGAAATAAAA TGTTTGTTCG GTTTGTTTCT GATGCATCTG TTCAAAGAAA AGGCTTTCAA 
GCTACACATT CTACAGAGTG TGGCGGACGA TTGAAAGCAG AATCAAAACC AAGAGATCTG 
TACTCACATG CTCAGTTTGG TGATAACAAC TACCCAGGAC AGGTTGACTG TGAATGGCTA 
TTAGTATCAG AACGGGGCTC TCGACTTGAA TTATCCTTCC AGACATTTGA AGTGGAGGAA 
GAAGCAGACT GTGGCTATGA CTATGTGGAG CTCTTTGATG GTCTTGATTC AACAGCTGTG 
GGGCTTGGTC GATTCTGTGG ATCCGGGCCA CCAGAAGAGA TTTATTCAAT TGGAGATTCA 
GTTTTAATTC ATTTCCACAC TGATGACACA ATCAACAAGA AGGGATTTCA TATAAGATAC 
AAAAGCATAA GATATCCAGA TACCACACAT ACCAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036596.3
CDS	669..3710
Translation	MGLGTLSPRMLVWLVASGIVFYGELWVCAGLDYDYTFDGNEEDKTETIDYKDPCKAAVFWGDIALDDEDLNIFQIDRTIDLTQNPFGNLGHTTGGLGDHAMSKKRGALYQLIDRIRRIGFGLEQNNTVKGKVPLQFSGQNEKNRVPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESNGNLSSPGFPNGYPSYTHCIWRVSVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKLPEVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVWKITVSESYHVGLTFQSFEIERHDNCAYDYLEVRDGTSENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVVAPTQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGAEVPEVITSQFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYMCQCRNGFVLHDNKHDCKEAECEQKIHSPSGLITSPNWPDKYPSRKECTWEISATPGHRIKLAFSEFEIEQHQECAYDHLEVFDGETEKSPILGRLCGNKIPDPLVATGNKMFVRFVSDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNNYPGQVDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVELFDGLDSTAVGLGRFCGSGPPEEIYSIGDSVLIHFHTDDTINKKGFHIRYKSIRYPDTTHTKK

Target ORF information:

RefSeq Version	NM_012464.5
Organism	Homo sapiens(human)
Definition	Homo sapiens tolloid like 1 (TLL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012464.5

ORF Insert Sequence:

ATGGGGTTGG GAACGCTTTC CCCGAGGATG CTCGTGTGGC TGGTGGCCTC GGGGATTGTT 
TTCTACGGGG AGCTATGGGT CTGCGCTGGC CTCGATTATG ATTACACTTT TGATGGGAAC 
GAAGAGGATA AAACAGAGAC TATAGATTAC AAGGACCCGT GTAAAGCCGC TGTATTTTGG 
GGCGATATTG CCTTAGATGA TGAAGACTTA AATATCTTTC AAATAGATAG GACAATTGAC 
CTTACGCAGA ACCCCTTTGG AAACCTTGGA CATACCACAG GTGGACTTGG AGACCATGCT 
ATGTCAAAGA AGCGAGGGGC CCTCTACCAA CTTATAGACA GGATAAGAAG AATTGGCTTT 
GGCTTGGAGC AAAACAACAC AGTTAAGGGA AAAGTACCTC TACAATTCTC AGGGCAAAAT 
GAGAAAAATC GAGTTCCCAG AGCCGCTACA TCAAGAACGG AAAGAATATG GCCTGGAGGC 
GTTATTCCTT ATGTTATAGG AGGAAACTTC ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGGC ACTGGGAAAA GCACACATGT GTGACTTTCA TAGAAAGAAG TGATGAAGAG 
AGTTACATTG TATTCACCTA TAGGCCTTGT GGATGCTGCT CCTATGTAGG TCGGCGAGGA 
AATGGACCTC AGGCAATCTC TATCGGCAAG AACTGTGATA AATTTGGGAT TGTTGTTCAT 
GAATTGGGTC ATGTGATAGG CTTTTGGCAT GAACACACAA GACCAGATCG AGATAACCAC 
GTAACTATCA TAAGAGAAAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT GAAGATGGAG 
CCTGGAGAAG TAAACTCACT TGGAGAAAGA TATGATTTCG ACAGTATCAT GCACTATGCC 
AGGAACACCT TCTCAAGGGG GATGTTTCTG GATACCATTC TCCCCTCCCG TGATGATAAT 
GGCATACGTC CTGCAATTGG TCAGCGAACC CGTCTAAGCA AAGGAGATAT CGCACAGGCA 
AGAAAGCTGT ATAGATGTCC AGCATGTGGA GAAACTCTAC AAGAATCCAA TGGCAACCTT 
TCCTCTCCAG GATTTCCCAA TGGCTACCCT TCTTACACAC ACTGCATCTG GAGAGTTTCT 
GTGACCCCAG GGGAGAAGAT TGTTTTAAAT TTTACAACGA TGGATCTATA CAAGAGTAGT 
TTGTGCTGGT ATGACTATAT TGAAGTAAGA GACGGGTACT GGAGAAAATC ACCTCTCCTT 
GGTAGATTCT GTGGGGACAA ATTGCCTGAA GTTCTTACTT CTACAGACAG CAGAATGTGG 
ATTGAGTTTC GTAGCAGCAG TAATTGGGTA GGAAAAGGCT TTGCAGCTGT CTATGAAGCG 
ATCTGTGGAG GTGAGATACG TAAAAATGAA GGACAGATTC AGTCTCCCAA TTATCCTGAT 
GACTATCGCC CGATGAAAGA ATGTGTGTGG AAAATAACAG TGTCTGAGAG CTACCACGTC 
GGGCTGACCT TTCAGTCCTT TGAGATTGAA AGACATGACA ATTGTGCTTA TGACTACCTG 
GAAGTTAGAG ATGGAACCAG TGAAAATAGC CCTTTGATAG GGCGTTTCTG TGGTTATGAC 
AAACCTGAAG ACATAAGATC TACCTCCAAT ACTTTGTGGA TGAAGTTTGT TTCTGACGGA 
ACTGTGAACA AAGCAGGGTT TGCTGCTAAC TTTTTTAAAG AGGAAGATGA GTGTGCCAAA 
CCTGACCGTG GAGGCTGTGA GCAGCGATGT CTGAACACTC TGGGCAGTTA CCAGTGTGCC 
TGTGAGCCTG GCTATGAGCT GGGCCCAGAC AGAAGGAGCT GTGAAGCTGC TTGTGGTGGA 
CTTCTTACCA AACTTAACGG CACCATAACC ACCCCTGGCT GGCCCAAGGA GTACCCTCCT 
AATAAGAACT GTGTGTGGCA AGTGGTTGCA CCAACCCAGT ACAGAATTTC TGTGAAGTTT 
GAGTTTTTTG AATTGGAAGG CAATGAAGTT TGCAAATATG ATTATGTGGA GATCTGGAGT 
GGTCTTTCCT CTGAGTCTAA ACTGCATGGC AAATTCTGTG GCGCTGAAGT GCCTGAAGTG 
ATCACATCCC AGTTCAACAA TATGAGAATT GAATTCAAAT CTGACAATAC TGTATCCAAG 
AAGGGCTTCA AAGCACATTT TTTCTCAGAC AAAGATGAAT GCTCTAAGGA TAATGGTGGA 
TGTCAGCACG AATGTGTCAA CACGATGGGG AGCTACATGT GTCAATGCCG TAATGGATTT 
GTGCTACATG ACAATAAACA TGATTGCAAG GAAGCTGAGT GTGAACAGAA GATCCACAGT 
CCAAGTGGCC TCATCACCAG TCCCAACTGG CCAGACAAGT ACCCAAGCAG GAAAGAATGC 
ACTTGGGAAA TCAGCGCCAC TCCTGGCCAC CGAATCAAAT TAGCCTTTAG TGAATTTGAG 
ATTGAGCAGC ATCAAGAATG TGCTTATGAC CACTTAGAAG TATTTGATGG AGAAACAGAA 
AAGTCACCGA TTCTTGGACG ACTGTGTGGC AACAAGATAC CAGATCCCCT TGTGGCTACT 
GGAAATAAAA TGTTTGTTCG GTTTGTTTCT GATGCATCTG TTCAAAGAAA AGGCTTTCAA 
GCTACACATT CTACAGAGTG TGGCGGACGA TTGAAAGCAG AATCAAAACC AAGAGATCTG 
TACTCACATG CTCAGTTTGG TGATAACAAC TACCCAGGAC AGGTTGACTG TGAATGGCTA 
TTAGTATCAG AACGGGGCTC TCGACTTGAA TTATCCTTCC AGACATTTGA AGTGGAGGAA 
GAAGCAGACT GTGGCTATGA CTATGTGGAG CTCTTTGATG GTCTTGATTC AACAGCTGTG 
GGGCTTGGTC GATTCTGTGG ATCCGGGCCA CCAGAAGAGA TTTATTCAAT TGGAGATTCA 
GTTTTAATTC ATTTCCACAC TGATGACACA ATCAACAAGA AGGGATTTCA TATAAGATAC 
AAAAGCATAA GATATCCAGA TACCACACAT ACCAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLL1 ( NM_012464.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036596.3 Homo sapiens tolloid like 1 (TLL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20767
Clone ID Related Accession (Same CDS sequence)	NM_012464.5 , NM_012464.4
Accession Version	NM_012464.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-27
Organism	Homo sapiens(human)
Product	tolloid-like protein 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U91963.1, AC097507.2, AC093874.3, BC144085.1, AF282732.1, AK021908.1 and BU753788.1. This sequence is a reference standard in the RefSeqGene project. On Mar 10, 2011 this sequence version replaced NM_012464.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U91963.1, SRR1803613.51262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTTGG GAACGCTTTC CCCGAGGATG CTCGTGTGGC TGGTGGCCTC GGGGATTGTT 
TTCTACGGGG AGCTATGGGT CTGCGCTGGC CTCGATTATG ATTACACTTT TGATGGGAAC 
GAAGAGGATA AAACAGAGAC TATAGATTAC AAGGACCCGT GTAAAGCCGC TGTATTTTGG 
GGCGATATTG CCTTAGATGA TGAAGACTTA AATATCTTTC AAATAGATAG GACAATTGAC 
CTTACGCAGA ACCCCTTTGG AAACCTTGGA CATACCACAG GTGGACTTGG AGACCATGCT 
ATGTCAAAGA AGCGAGGGGC CCTCTACCAA CTTATAGACA GGATAAGAAG AATTGGCTTT 
GGCTTGGAGC AAAACAACAC AGTTAAGGGA AAAGTACCTC TACAATTCTC AGGGCAAAAT 
GAGAAAAATC GAGTTCCCAG AGCCGCTACA TCAAGAACGG AAAGAATATG GCCTGGAGGC 
GTTATTCCTT ATGTTATAGG AGGAAACTTC ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGGC ACTGGGAAAA GCACACATGT GTGACTTTCA TAGAAAGAAG TGATGAAGAG 
AGTTACATTG TATTCACCTA TAGGCCTTGT GGATGCTGCT CCTATGTAGG TCGGCGAGGA 
AATGGACCTC AGGCAATCTC TATCGGCAAG AACTGTGATA AATTTGGGAT TGTTGTTCAT 
GAATTGGGTC ATGTGATAGG CTTTTGGCAT GAACACACAA GACCAGATCG AGATAACCAC 
GTAACTATCA TAAGAGAAAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT GAAGATGGAG 
CCTGGAGAAG TAAACTCACT TGGAGAAAGA TATGATTTCG ACAGTATCAT GCACTATGCC 
AGGAACACCT TCTCAAGGGG GATGTTTCTG GATACCATTC TCCCCTCCCG TGATGATAAT 
GGCATACGTC CTGCAATTGG TCAGCGAACC CGTCTAAGCA AAGGAGATAT CGCACAGGCA 
AGAAAGCTGT ATAGATGTCC AGCATGTGGA GAAACTCTAC AAGAATCCAA TGGCAACCTT 
TCCTCTCCAG GATTTCCCAA TGGCTACCCT TCTTACACAC ACTGCATCTG GAGAGTTTCT 
GTGACCCCAG GGGAGAAGAT TGTTTTAAAT TTTACAACGA TGGATCTATA CAAGAGTAGT 
TTGTGCTGGT ATGACTATAT TGAAGTAAGA GACGGGTACT GGAGAAAATC ACCTCTCCTT 
GGTAGATTCT GTGGGGACAA ATTGCCTGAA GTTCTTACTT CTACAGACAG CAGAATGTGG 
ATTGAGTTTC GTAGCAGCAG TAATTGGGTA GGAAAAGGCT TTGCAGCTGT CTATGAAGCG 
ATCTGTGGAG GTGAGATACG TAAAAATGAA GGACAGATTC AGTCTCCCAA TTATCCTGAT 
GACTATCGCC CGATGAAAGA ATGTGTGTGG AAAATAACAG TGTCTGAGAG CTACCACGTC 
GGGCTGACCT TTCAGTCCTT TGAGATTGAA AGACATGACA ATTGTGCTTA TGACTACCTG 
GAAGTTAGAG ATGGAACCAG TGAAAATAGC CCTTTGATAG GGCGTTTCTG TGGTTATGAC 
AAACCTGAAG ACATAAGATC TACCTCCAAT ACTTTGTGGA TGAAGTTTGT TTCTGACGGA 
ACTGTGAACA AAGCAGGGTT TGCTGCTAAC TTTTTTAAAG AGGAAGATGA GTGTGCCAAA 
CCTGACCGTG GAGGCTGTGA GCAGCGATGT CTGAACACTC TGGGCAGTTA CCAGTGTGCC 
TGTGAGCCTG GCTATGAGCT GGGCCCAGAC AGAAGGAGCT GTGAAGCTGC TTGTGGTGGA 
CTTCTTACCA AACTTAACGG CACCATAACC ACCCCTGGCT GGCCCAAGGA GTACCCTCCT 
AATAAGAACT GTGTGTGGCA AGTGGTTGCA CCAACCCAGT ACAGAATTTC TGTGAAGTTT 
GAGTTTTTTG AATTGGAAGG CAATGAAGTT TGCAAATATG ATTATGTGGA GATCTGGAGT 
GGTCTTTCCT CTGAGTCTAA ACTGCATGGC AAATTCTGTG GCGCTGAAGT GCCTGAAGTG 
ATCACATCCC AGTTCAACAA TATGAGAATT GAATTCAAAT CTGACAATAC TGTATCCAAG 
AAGGGCTTCA AAGCACATTT TTTCTCAGAC AAAGATGAAT GCTCTAAGGA TAATGGTGGA 
TGTCAGCACG AATGTGTCAA CACGATGGGG AGCTACATGT GTCAATGCCG TAATGGATTT 
GTGCTACATG ACAATAAACA TGATTGCAAG GAAGCTGAGT GTGAACAGAA GATCCACAGT 
CCAAGTGGCC TCATCACCAG TCCCAACTGG CCAGACAAGT ACCCAAGCAG GAAAGAATGC 
ACTTGGGAAA TCAGCGCCAC TCCTGGCCAC CGAATCAAAT TAGCCTTTAG TGAATTTGAG 
ATTGAGCAGC ATCAAGAATG TGCTTATGAC CACTTAGAAG TATTTGATGG AGAAACAGAA 
AAGTCACCGA TTCTTGGACG ACTGTGTGGC AACAAGATAC CAGATCCCCT TGTGGCTACT 
GGAAATAAAA TGTTTGTTCG GTTTGTTTCT GATGCATCTG TTCAAAGAAA AGGCTTTCAA 
GCTACACATT CTACAGAGTG TGGCGGACGA TTGAAAGCAG AATCAAAACC AAGAGATCTG 
TACTCACATG CTCAGTTTGG TGATAACAAC TACCCAGGAC AGGTTGACTG TGAATGGCTA 
TTAGTATCAG AACGGGGCTC TCGACTTGAA TTATCCTTCC AGACATTTGA AGTGGAGGAA 
GAAGCAGACT GTGGCTATGA CTATGTGGAG CTCTTTGATG GTCTTGATTC AACAGCTGTG 
GGGCTTGGTC GATTCTGTGG ATCCGGGCCA CCAGAAGAGA TTTATTCAAT TGGAGATTCA 
GTTTTAATTC ATTTCCACAC TGATGACACA ATCAACAAGA AGGGATTTCA TATAAGATAC 
AAAAGCATAA GATATCCAGA TACCACACAT ACCAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036596.3
CDS	648..3689
Translation	MGLGTLSPRMLVWLVASGIVFYGELWVCAGLDYDYTFDGNEEDKTETIDYKDPCKAAVFWGDIALDDEDLNIFQIDRTIDLTQNPFGNLGHTTGGLGDHAMSKKRGALYQLIDRIRRIGFGLEQNNTVKGKVPLQFSGQNEKNRVPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESNGNLSSPGFPNGYPSYTHCIWRVSVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKLPEVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVWKITVSESYHVGLTFQSFEIERHDNCAYDYLEVRDGTSENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVVAPTQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGAEVPEVITSQFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYMCQCRNGFVLHDNKHDCKEAECEQKIHSPSGLITSPNWPDKYPSRKECTWEISATPGHRIKLAFSEFEIEQHQECAYDHLEVFDGETEKSPILGRLCGNKIPDPLVATGNKMFVRFVSDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNNYPGQVDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVELFDGLDSTAVGLGRFCGSGPPEEIYSIGDSVLIHFHTDDTINKKGFHIRYKSIRYPDTTHTKK

Target ORF information:

RefSeq Version	NM_012464.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tolloid like 1 (TLL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012464.4

ORF Insert Sequence:

ATGGGGTTGG GAACGCTTTC CCCGAGGATG CTCGTGTGGC TGGTGGCCTC GGGGATTGTT 
TTCTACGGGG AGCTATGGGT CTGCGCTGGC CTCGATTATG ATTACACTTT TGATGGGAAC 
GAAGAGGATA AAACAGAGAC TATAGATTAC AAGGACCCGT GTAAAGCCGC TGTATTTTGG 
GGCGATATTG CCTTAGATGA TGAAGACTTA AATATCTTTC AAATAGATAG GACAATTGAC 
CTTACGCAGA ACCCCTTTGG AAACCTTGGA CATACCACAG GTGGACTTGG AGACCATGCT 
ATGTCAAAGA AGCGAGGGGC CCTCTACCAA CTTATAGACA GGATAAGAAG AATTGGCTTT 
GGCTTGGAGC AAAACAACAC AGTTAAGGGA AAAGTACCTC TACAATTCTC AGGGCAAAAT 
GAGAAAAATC GAGTTCCCAG AGCCGCTACA TCAAGAACGG AAAGAATATG GCCTGGAGGC 
GTTATTCCTT ATGTTATAGG AGGAAACTTC ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGGC ACTGGGAAAA GCACACATGT GTGACTTTCA TAGAAAGAAG TGATGAAGAG 
AGTTACATTG TATTCACCTA TAGGCCTTGT GGATGCTGCT CCTATGTAGG TCGGCGAGGA 
AATGGACCTC AGGCAATCTC TATCGGCAAG AACTGTGATA AATTTGGGAT TGTTGTTCAT 
GAATTGGGTC ATGTGATAGG CTTTTGGCAT GAACACACAA GACCAGATCG AGATAACCAC 
GTAACTATCA TAAGAGAAAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT GAAGATGGAG 
CCTGGAGAAG TAAACTCACT TGGAGAAAGA TATGATTTCG ACAGTATCAT GCACTATGCC 
AGGAACACCT TCTCAAGGGG GATGTTTCTG GATACCATTC TCCCCTCCCG TGATGATAAT 
GGCATACGTC CTGCAATTGG TCAGCGAACC CGTCTAAGCA AAGGAGATAT CGCACAGGCA 
AGAAAGCTGT ATAGATGTCC AGCATGTGGA GAAACTCTAC AAGAATCCAA TGGCAACCTT 
TCCTCTCCAG GATTTCCCAA TGGCTACCCT TCTTACACAC ACTGCATCTG GAGAGTTTCT 
GTGACCCCAG GGGAGAAGAT TGTTTTAAAT TTTACAACGA TGGATCTATA CAAGAGTAGT 
TTGTGCTGGT ATGACTATAT TGAAGTAAGA GACGGGTACT GGAGAAAATC ACCTCTCCTT 
GGTAGATTCT GTGGGGACAA ATTGCCTGAA GTTCTTACTT CTACAGACAG CAGAATGTGG 
ATTGAGTTTC GTAGCAGCAG TAATTGGGTA GGAAAAGGCT TTGCAGCTGT CTATGAAGCG 
ATCTGTGGAG GTGAGATACG TAAAAATGAA GGACAGATTC AGTCTCCCAA TTATCCTGAT 
GACTATCGCC CGATGAAAGA ATGTGTGTGG AAAATAACAG TGTCTGAGAG CTACCACGTC 
GGGCTGACCT TTCAGTCCTT TGAGATTGAA AGACATGACA ATTGTGCTTA TGACTACCTG 
GAAGTTAGAG ATGGAACCAG TGAAAATAGC CCTTTGATAG GGCGTTTCTG TGGTTATGAC 
AAACCTGAAG ACATAAGATC TACCTCCAAT ACTTTGTGGA TGAAGTTTGT TTCTGACGGA 
ACTGTGAACA AAGCAGGGTT TGCTGCTAAC TTTTTTAAAG AGGAAGATGA GTGTGCCAAA 
CCTGACCGTG GAGGCTGTGA GCAGCGATGT CTGAACACTC TGGGCAGTTA CCAGTGTGCC 
TGTGAGCCTG GCTATGAGCT GGGCCCAGAC AGAAGGAGCT GTGAAGCTGC TTGTGGTGGA 
CTTCTTACCA AACTTAACGG CACCATAACC ACCCCTGGCT GGCCCAAGGA GTACCCTCCT 
AATAAGAACT GTGTGTGGCA AGTGGTTGCA CCAACCCAGT ACAGAATTTC TGTGAAGTTT 
GAGTTTTTTG AATTGGAAGG CAATGAAGTT TGCAAATATG ATTATGTGGA GATCTGGAGT 
GGTCTTTCCT CTGAGTCTAA ACTGCATGGC AAATTCTGTG GCGCTGAAGT GCCTGAAGTG 
ATCACATCCC AGTTCAACAA TATGAGAATT GAATTCAAAT CTGACAATAC TGTATCCAAG 
AAGGGCTTCA AAGCACATTT TTTCTCAGAC AAAGATGAAT GCTCTAAGGA TAATGGTGGA 
TGTCAGCACG AATGTGTCAA CACGATGGGG AGCTACATGT GTCAATGCCG TAATGGATTT 
GTGCTACATG ACAATAAACA TGATTGCAAG GAAGCTGAGT GTGAACAGAA GATCCACAGT 
CCAAGTGGCC TCATCACCAG TCCCAACTGG CCAGACAAGT ACCCAAGCAG GAAAGAATGC 
ACTTGGGAAA TCAGCGCCAC TCCTGGCCAC CGAATCAAAT TAGCCTTTAG TGAATTTGAG 
ATTGAGCAGC ATCAAGAATG TGCTTATGAC CACTTAGAAG TATTTGATGG AGAAACAGAA 
AAGTCACCGA TTCTTGGACG ACTGTGTGGC AACAAGATAC CAGATCCCCT TGTGGCTACT 
GGAAATAAAA TGTTTGTTCG GTTTGTTTCT GATGCATCTG TTCAAAGAAA AGGCTTTCAA 
GCTACACATT CTACAGAGTG TGGCGGACGA TTGAAAGCAG AATCAAAACC AAGAGATCTG 
TACTCACATG CTCAGTTTGG TGATAACAAC TACCCAGGAC AGGTTGACTG TGAATGGCTA 
TTAGTATCAG AACGGGGCTC TCGACTTGAA TTATCCTTCC AGACATTTGA AGTGGAGGAA 
GAAGCAGACT GTGGCTATGA CTATGTGGAG CTCTTTGATG GTCTTGATTC AACAGCTGTG 
GGGCTTGGTC GATTCTGTGG ATCCGGGCCA CCAGAAGAGA TTTATTCAAT TGGAGATTCA 
GTTTTAATTC ATTTCCACAC TGATGACACA ATCAACAAGA AGGGATTTCA TATAAGATAC 
AAAAGCATAA GATATCCAGA TACCACACAT ACCAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLL1 ( NM_001204760.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191689.1 Homo sapiens tolloid like 1 (TLL1), transcript variant 2, mRNA.

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CloneID	OHu17668
Clone ID Related Accession (Same CDS sequence)	NM_001204760.1 , NM_001204760.2
Accession Version	NM_001204760.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	tolloid-like protein 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U91963.1 and BC016922.2. Transcript Variant: This variant (2) is missing many exons from the 3' end, and contains a novel 3' terminal exon compared to variant 1. This results in a frame-shift, and a much shorter isoform (2) with a distinct C-terminus compared to isoform 1. This isoform lacks experimental support. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016922.2, SRR1803616.127078.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTTGG GAACGCTTTC CCCGAGGATG CTCGTGTGGC TGGTGGCCTC GGGGATTGTT 
TTCTACGGGG AGCTATGGGT CTGCGCTGGC CTCGATTATG ATTACACTTT TGATGGGAAC 
GAAGAGGATA AAACAGAGAC TATAGATTAC AAGGACCCGT GTAAAGCCGC TGTATTTTGG 
GGCGATATTG CCTTAGATGA TGAAGACTTA AATATCTTTC AAATAGATAG GACAATTGAC 
CTTACGCAGA ACCCCTTTGG AAACCTTGGA CATACCACAG GTGGACTTGG AGACCATGCT 
ATGTCAAAGA AGCGAGGGGC CCTCTACCAA CTTATAGACA GGATAAGAAG AATTGGCTTT 
GGCTTGGAGC AAAACAACAC AGTTAAGGGA AAAGTACCTC TACAATTCTC AGGGCAAAAT 
GAGAAAAATC GAGTTCCCAG AGCCGCTACA TCAAGAACGG AAAGAATATG GCCTGGAGGC 
GTTATTCCTT ATGTTATAGG AGGAAACTTC ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGGC ACTGGGAAAA GCACACATGT GTGACTTTCA TAGAAAGAAG TGATGAAGAG 
AGTTACATTG TATTCACCTA TAGGCCTTGT GGATGCTGCT CCTATGTAGG TCGGCGAGGA 
AATGGACCTC AGGCAATCTC TATCGGCAAG AACTGTGATA AATTTGGGAT TGTTGTTCAT 
GAATTGGGTC ATGTGATAGG CTTTTGGCAT GAACACACAA GACCAGATCG AGATAACCAC 
GTAACTATCA TAAGAGAAAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT GAAGATGGAG 
CCTGGAGAAG TAAACTCACT TGGAGAAAGA TATGATTTCG ACAGTATCAT GCACTATGCC 
AGGAACACCT TCTCAAGGGG GATGTTTCTG GATACCATTC TCCCCTCCCG TGATGATAAT 
GGCATACGTC CTGCAATTGG TCAGCGAACC CGTCTAAGCA AAGGAGATAT CGCACAGGCA 
AGAAAGCTGT ATAGATGTCC AGCATGTGGA GAAACTCTAC AAGAATCCAA TGGCAACCTT 
TCCTCTCCAG GATTTCCCAA TGGCTACCCT TCTTACACAC ACTGCATCTG GAGAGTTTCT 
GTGACCCCAG GGGAGAAGGT TGTATTTTCA CTCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191689.1
CDS	648..1826
Translation	MGLGTLSPRMLVWLVASGIVFYGELWVCAGLDYDYTFDGNEEDKTETIDYKDPCKAAVFWGDIALDDEDLNIFQIDRTIDLTQNPFGNLGHTTGGLGDHAMSKKRGALYQLIDRIRRIGFGLEQNNTVKGKVPLQFSGQNEKNRVPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESNGNLSSPGFPNGYPSYTHCIWRVSVTPGEKVVFSLC

Target ORF information:

RefSeq Version	NM_001204760.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tolloid like 1 (TLL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204760.1

ORF Insert Sequence:

ATGGGGTTGG GAACGCTTTC CCCGAGGATG CTCGTGTGGC TGGTGGCCTC GGGGATTGTT 
TTCTACGGGG AGCTATGGGT CTGCGCTGGC CTCGATTATG ATTACACTTT TGATGGGAAC 
GAAGAGGATA AAACAGAGAC TATAGATTAC AAGGACCCGT GTAAAGCCGC TGTATTTTGG 
GGCGATATTG CCTTAGATGA TGAAGACTTA AATATCTTTC AAATAGATAG GACAATTGAC 
CTTACGCAGA ACCCCTTTGG AAACCTTGGA CATACCACAG GTGGACTTGG AGACCATGCT 
ATGTCAAAGA AGCGAGGGGC CCTCTACCAA CTTATAGACA GGATAAGAAG AATTGGCTTT 
GGCTTGGAGC AAAACAACAC AGTTAAGGGA AAAGTACCTC TACAATTCTC AGGGCAAAAT 
GAGAAAAATC GAGTTCCCAG AGCCGCTACA TCAAGAACGG AAAGAATATG GCCTGGAGGC 
GTTATTCCTT ATGTTATAGG AGGAAACTTC ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGGC ACTGGGAAAA GCACACATGT GTGACTTTCA TAGAAAGAAG TGATGAAGAG 
AGTTACATTG TATTCACCTA TAGGCCTTGT GGATGCTGCT CCTATGTAGG TCGGCGAGGA 
AATGGACCTC AGGCAATCTC TATCGGCAAG AACTGTGATA AATTTGGGAT TGTTGTTCAT 
GAATTGGGTC ATGTGATAGG CTTTTGGCAT GAACACACAA GACCAGATCG AGATAACCAC 
GTAACTATCA TAAGAGAAAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT GAAGATGGAG 
CCTGGAGAAG TAAACTCACT TGGAGAAAGA TATGATTTCG ACAGTATCAT GCACTATGCC 
AGGAACACCT TCTCAAGGGG GATGTTTCTG GATACCATTC TCCCCTCCCG TGATGATAAT 
GGCATACGTC CTGCAATTGG TCAGCGAACC CGTCTAAGCA AAGGAGATAT CGCACAGGCA 
AGAAAGCTGT ATAGATGTCC AGCATGTGGA GAAACTCTAC AAGAATCCAA TGGCAACCTT 
TCCTCTCCAG GATTTCCCAA TGGCTACCCT TCTTACACAC ACTGCATCTG GAGAGTTTCT 
GTGACCCCAG GGGAGAAGGT TGTATTTTCA CTCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLL1 ( NM_001204760.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191689.1 Homo sapiens tolloid like 1 (TLL1), transcript variant 2, mRNA.

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CloneID	OHu17668
Clone ID Related Accession (Same CDS sequence)	NM_001204760.1 , NM_001204760.2
Accession Version	NM_001204760.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tolloid-like protein 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC097502.3, U91963.1 and BC016922.2. On May 31, 2019 this sequence version replaced NM_001204760.1. Transcript Variant: This variant (2) is missing many exons from the 3' end, and contains a novel 3' terminal exon compared to variant 1. This results in a frame-shift, and a much shorter isoform (2) with a distinct C-terminus compared to isoform 1. This isoform lacks experimental support. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016922.2, SRR1803616.127078.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTTGG GAACGCTTTC CCCGAGGATG CTCGTGTGGC TGGTGGCCTC GGGGATTGTT 
TTCTACGGGG AGCTATGGGT CTGCGCTGGC CTCGATTATG ATTACACTTT TGATGGGAAC 
GAAGAGGATA AAACAGAGAC TATAGATTAC AAGGACCCGT GTAAAGCCGC TGTATTTTGG 
GGCGATATTG CCTTAGATGA TGAAGACTTA AATATCTTTC AAATAGATAG GACAATTGAC 
CTTACGCAGA ACCCCTTTGG AAACCTTGGA CATACCACAG GTGGACTTGG AGACCATGCT 
ATGTCAAAGA AGCGAGGGGC CCTCTACCAA CTTATAGACA GGATAAGAAG AATTGGCTTT 
GGCTTGGAGC AAAACAACAC AGTTAAGGGA AAAGTACCTC TACAATTCTC AGGGCAAAAT 
GAGAAAAATC GAGTTCCCAG AGCCGCTACA TCAAGAACGG AAAGAATATG GCCTGGAGGC 
GTTATTCCTT ATGTTATAGG AGGAAACTTC ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGGC ACTGGGAAAA GCACACATGT GTGACTTTCA TAGAAAGAAG TGATGAAGAG 
AGTTACATTG TATTCACCTA TAGGCCTTGT GGATGCTGCT CCTATGTAGG TCGGCGAGGA 
AATGGACCTC AGGCAATCTC TATCGGCAAG AACTGTGATA AATTTGGGAT TGTTGTTCAT 
GAATTGGGTC ATGTGATAGG CTTTTGGCAT GAACACACAA GACCAGATCG AGATAACCAC 
GTAACTATCA TAAGAGAAAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT GAAGATGGAG 
CCTGGAGAAG TAAACTCACT TGGAGAAAGA TATGATTTCG ACAGTATCAT GCACTATGCC 
AGGAACACCT TCTCAAGGGG GATGTTTCTG GATACCATTC TCCCCTCCCG TGATGATAAT 
GGCATACGTC CTGCAATTGG TCAGCGAACC CGTCTAAGCA AAGGAGATAT CGCACAGGCA 
AGAAAGCTGT ATAGATGTCC AGCATGTGGA GAAACTCTAC AAGAATCCAA TGGCAACCTT 
TCCTCTCCAG GATTTCCCAA TGGCTACCCT TCTTACACAC ACTGCATCTG GAGAGTTTCT 
GTGACCCCAG GGGAGAAGGT TGTATTTTCA CTCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191689.1
CDS	669..1847
Translation	MGLGTLSPRMLVWLVASGIVFYGELWVCAGLDYDYTFDGNEEDKTETIDYKDPCKAAVFWGDIALDDEDLNIFQIDRTIDLTQNPFGNLGHTTGGLGDHAMSKKRGALYQLIDRIRRIGFGLEQNNTVKGKVPLQFSGQNEKNRVPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESNGNLSSPGFPNGYPSYTHCIWRVSVTPGEKVVFSLC

Target ORF information:

RefSeq Version	NM_001204760.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tolloid like 1 (TLL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204760.2

ORF Insert Sequence:

ATGGGGTTGG GAACGCTTTC CCCGAGGATG CTCGTGTGGC TGGTGGCCTC GGGGATTGTT 
TTCTACGGGG AGCTATGGGT CTGCGCTGGC CTCGATTATG ATTACACTTT TGATGGGAAC 
GAAGAGGATA AAACAGAGAC TATAGATTAC AAGGACCCGT GTAAAGCCGC TGTATTTTGG 
GGCGATATTG CCTTAGATGA TGAAGACTTA AATATCTTTC AAATAGATAG GACAATTGAC 
CTTACGCAGA ACCCCTTTGG AAACCTTGGA CATACCACAG GTGGACTTGG AGACCATGCT 
ATGTCAAAGA AGCGAGGGGC CCTCTACCAA CTTATAGACA GGATAAGAAG AATTGGCTTT 
GGCTTGGAGC AAAACAACAC AGTTAAGGGA AAAGTACCTC TACAATTCTC AGGGCAAAAT 
GAGAAAAATC GAGTTCCCAG AGCCGCTACA TCAAGAACGG AAAGAATATG GCCTGGAGGC 
GTTATTCCTT ATGTTATAGG AGGAAACTTC ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGGC ACTGGGAAAA GCACACATGT GTGACTTTCA TAGAAAGAAG TGATGAAGAG 
AGTTACATTG TATTCACCTA TAGGCCTTGT GGATGCTGCT CCTATGTAGG TCGGCGAGGA 
AATGGACCTC AGGCAATCTC TATCGGCAAG AACTGTGATA AATTTGGGAT TGTTGTTCAT 
GAATTGGGTC ATGTGATAGG CTTTTGGCAT GAACACACAA GACCAGATCG AGATAACCAC 
GTAACTATCA TAAGAGAAAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT GAAGATGGAG 
CCTGGAGAAG TAAACTCACT TGGAGAAAGA TATGATTTCG ACAGTATCAT GCACTATGCC 
AGGAACACCT TCTCAAGGGG GATGTTTCTG GATACCATTC TCCCCTCCCG TGATGATAAT 
GGCATACGTC CTGCAATTGG TCAGCGAACC CGTCTAAGCA AAGGAGATAT CGCACAGGCA 
AGAAAGCTGT ATAGATGTCC AGCATGTGGA GAAACTCTAC AAGAATCCAA TGGCAACCTT 
TCCTCTCCAG GATTTCCCAA TGGCTACCCT TCTTACACAC ACTGCATCTG GAGAGTTTCT 
GTGACCCCAG GGGAGAAGGT TGTATTTTCA CTCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLL1 ( XM_011532214.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530516.1 Homo sapiens tolloid like 1 (TLL1), transcript variant X4, mRNA.

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CloneID	OHu61040
Clone ID Related Accession (Same CDS sequence)	XM_011532214.1
Accession Version	XM_011532214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tolloid-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAAGC AGGCCATGAG GCACTGGGAA AAGCACACAT GTGTGACTTT CATAGAAAGA 
AGTGATGAAG AGAGTTACAT TGTATTCACC TATAGGCCTT GTGGATGCTG CTCCTATGTA 
GGTCGGCGAG GAAATGGACC TCAGGCAATC TCTATCGGCA AGAACTGTGA TAAATTTGGG 
ATTGTTGTTC ATGAATTGGG TCATGTGATA GGCTTTTGGC ATGAACACAC AAGACCAGAT 
CGAGATAACC ACGTAACTAT CATAAGAGAA AACATCCAGC CAGGTCAAGA GTACAATTTT 
CTGAAGATGG AGCCTGGAGA AGTAAACTCA CTTGGAGAAA GATATGATTT CGACAGTATC 
ATGCACTATG CCAGGAACAC CTTCTCAAGG GGGATGTTTC TGGATACCAT TCTCCCCTCC 
CGTGATGATA ATGGCATACG TCCTGCAATT GGTCAGCGAA CCCGTCTAAG CAAAGGAGAT 
ATCGCACAGG CAAGAAAGCT GTATAGATGT CCAGCATGTG GAGAAACTCT ACAAGAATCC 
AATGGCAACC TTTCCTCTCC AGGATTTCCC AATGGCTACC CTTCTTACAC ACACTGCATC 
TGGAGAGTTT CTGTGACCCC AGGGGAGAAG ATTGTTTTAA ATTTTACAAC GATGGATCTA 
TACAAGAGTA GTTTGTGCTG GTATGACTAT ATTGAAGTAA GAGACGGGTA CTGGAGAAAA 
TCACCTCTCC TTGGTAGATT CTGTGGGGAC AAATTGCCTG AAGTTCTTAC TTCTACAGAC 
AGCAGAATGT GGATTGAGTT TCGTAGCAGC AGTAATTGGG TAGGAAAAGG CTTTGCAGCT 
GTCTATGAAG CGATCTGTGG AGGTGAGATA CGTAAAAATG AAGGACAGAT TCAGTCTCCC 
AATTATCCTG ATGACTATCG CCCGATGAAA GAATGTGTGT GGAAAATAAC AGTGTCTGAG 
AGCTACCACG TCGGGCTGAC CTTTCAGTCC TTTGAGATTG AAAGACATGA CAATTGTGCT 
TATGACTACC TGGAAGTTAG AGATGGAACC AGTGAAAATA GCCCTTTGAT AGGGCGTTTC 
TGTGGTTATG ACAAACCTGA AGACATAAGA TCTACCTCCA ATACTTTGTG GATGAAGTTT 
GTTTCTGACG GAACTGTGAA CAAAGCAGGG TTTGCTGCTA ACTTTTTTAA AGAGGAAGAT 
GAGTGTGCCA AACCTGACCG TGGAGGCTGT GAGCAGCGAT GTCTGAACAC TCTGGGCAGT 
TACCAGTGTG CCTGTGAGCC TGGCTATGAG CTGGGCCCAG ACAGAAGGAG CTGTGAAGCT 
GCTTGTGGTG GACTTCTTAC CAAACTTAAC GGCACCATAA CCACCCCTGG CTGGCCCAAG 
GAGTACCCTC CTAATAAGAA CTGTGTGTGG CAAGTGGTTG CACCAACCCA GTACAGAATT 
TCTGTGAAGT TTGAGTTTTT TGAATTGGAA GGCAATGAAG TTTGCAAATA TGATTATGTG 
GAGATCTGGA GTGGTCTTTC CTCTGAGTCT AAACTGCATG GCAAATTCTG TGGCGCTGAA 
GTGCCTGAAG TGATCACATC CCAGTTCAAC AATATGAGAA TTGAATTCAA ATCTGACAAT 
ACTGTATCCA AGAAGGGCTT CAAAGCACAT TTTTTCTCAG ACAAAGATGA ATGCTCTAAG 
GATAATGGTG GATGTCAGCA CGAATGTGTC AACACGATGG GGAGCTACAT GTGTCAATGC 
CGTAATGGAT TTGTGCTACA TGACAATAAA CATGATTGCA AGGAAGCTGA GTGTGAACAG 
AAGATCCACA GTCCAAGTGG CCTCATCACC AGTCCCAACT GGCCAGACAA GTACCCAAGC 
AGGAAAGAAT GCACTTGGGA AATCAGCGCC ACTCCTGGCC ACCGAATCAA ATTAGCCTTT 
AGTGAATTTG AGATTGAGCA GCATCAAGAA TGTGCTTATG ACCACTTAGA AGTATTTGAT 
GGAGAAACAG AAAAGTCACC GATTCTTGGA CGACTGTGTG GCAACAAGAT ACCAGATCCC 
CTTGTGGCTA CTGGAAATAA AATGTTTGTT CGGTTTGTTT CTGATGCATC TGTTCAAAGA 
AAAGGCTTTC AAGCTACACA TTCTACAGAG TGTGGCGGAC GATTGAAAGC AGAATCAAAA 
CCAAGAGATC TGTACTCACA TGCTCAGTTT GGTGATAACA ACTACCCAGG ACAGGTTGAC 
TGTGAATGGC TATTAGTATC AGAACGGGGC TCTCGACTTG AATTATCCTT CCAGACATTT 
GAAGTGGAGG AAGAAGCAGA CTGTGGCTAT GACTATGTGG AGCTCTTTGA TGGTCTTGAT 
TCAACAGCTG TGGGGCTTGG TCGATTCTGT GGATCCGGGC CACCAGAAGA GATTTATTCA 
ATTGGAGATT CAGTTTTAAT TCATTTCCAC ACTGATGACA CAATCAACAA GAAGGGATTT 
CATATAAGAT ACAAAAGCAT AAGATATCCA GATACCACAC ATACCAAAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530516.1
CDS	1234..3747
Translation	MFKQAMRHWEKHTCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESNGNLSSPGFPNGYPSYTHCIWRVSVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKLPEVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVWKITVSESYHVGLTFQSFEIERHDNCAYDYLEVRDGTSENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVVAPTQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGAEVPEVITSQFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYMCQCRNGFVLHDNKHDCKEAECEQKIHSPSGLITSPNWPDKYPSRKECTWEISATPGHRIKLAFSEFEIEQHQECAYDHLEVFDGETEKSPILGRLCGNKIPDPLVATGNKMFVRFVSDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNNYPGQVDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVELFDGLDSTAVGLGRFCGSGPPEEIYSIGDSVLIHFHTDDTINKKGFHIRYKSIRYPDTTHTKK

Target ORF information:

RefSeq Version	XM_011532214.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tolloid like 1 (TLL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532214.1

ORF Insert Sequence:

ATGTTCAAGC AGGCCATGAG GCACTGGGAA AAGCACACAT GTGTGACTTT CATAGAAAGA 
AGTGATGAAG AGAGTTACAT TGTATTCACC TATAGGCCTT GTGGATGCTG CTCCTATGTA 
GGTCGGCGAG GAAATGGACC TCAGGCAATC TCTATCGGCA AGAACTGTGA TAAATTTGGG 
ATTGTTGTTC ATGAATTGGG TCATGTGATA GGCTTTTGGC ATGAACACAC AAGACCAGAT 
CGAGATAACC ACGTAACTAT CATAAGAGAA AACATCCAGC CAGGTCAAGA GTACAATTTT 
CTGAAGATGG AGCCTGGAGA AGTAAACTCA CTTGGAGAAA GATATGATTT CGACAGTATC 
ATGCACTATG CCAGGAACAC CTTCTCAAGG GGGATGTTTC TGGATACCAT TCTCCCCTCC 
CGTGATGATA ATGGCATACG TCCTGCAATT GGTCAGCGAA CCCGTCTAAG CAAAGGAGAT 
ATCGCACAGG CAAGAAAGCT GTATAGATGT CCAGCATGTG GAGAAACTCT ACAAGAATCC 
AATGGCAACC TTTCCTCTCC AGGATTTCCC AATGGCTACC CTTCTTACAC ACACTGCATC 
TGGAGAGTTT CTGTGACCCC AGGGGAGAAG ATTGTTTTAA ATTTTACAAC GATGGATCTA 
TACAAGAGTA GTTTGTGCTG GTATGACTAT ATTGAAGTAA GAGACGGGTA CTGGAGAAAA 
TCACCTCTCC TTGGTAGATT CTGTGGGGAC AAATTGCCTG AAGTTCTTAC TTCTACAGAC 
AGCAGAATGT GGATTGAGTT TCGTAGCAGC AGTAATTGGG TAGGAAAAGG CTTTGCAGCT 
GTCTATGAAG CGATCTGTGG AGGTGAGATA CGTAAAAATG AAGGACAGAT TCAGTCTCCC 
AATTATCCTG ATGACTATCG CCCGATGAAA GAATGTGTGT GGAAAATAAC AGTGTCTGAG 
AGCTACCACG TCGGGCTGAC CTTTCAGTCC TTTGAGATTG AAAGACATGA CAATTGTGCT 
TATGACTACC TGGAAGTTAG AGATGGAACC AGTGAAAATA GCCCTTTGAT AGGGCGTTTC 
TGTGGTTATG ACAAACCTGA AGACATAAGA TCTACCTCCA ATACTTTGTG GATGAAGTTT 
GTTTCTGACG GAACTGTGAA CAAAGCAGGG TTTGCTGCTA ACTTTTTTAA AGAGGAAGAT 
GAGTGTGCCA AACCTGACCG TGGAGGCTGT GAGCAGCGAT GTCTGAACAC TCTGGGCAGT 
TACCAGTGTG CCTGTGAGCC TGGCTATGAG CTGGGCCCAG ACAGAAGGAG CTGTGAAGCT 
GCTTGTGGTG GACTTCTTAC CAAACTTAAC GGCACCATAA CCACCCCTGG CTGGCCCAAG 
GAGTACCCTC CTAATAAGAA CTGTGTGTGG CAAGTGGTTG CACCAACCCA GTACAGAATT 
TCTGTGAAGT TTGAGTTTTT TGAATTGGAA GGCAATGAAG TTTGCAAATA TGATTATGTG 
GAGATCTGGA GTGGTCTTTC CTCTGAGTCT AAACTGCATG GCAAATTCTG TGGCGCTGAA 
GTGCCTGAAG TGATCACATC CCAGTTCAAC AATATGAGAA TTGAATTCAA ATCTGACAAT 
ACTGTATCCA AGAAGGGCTT CAAAGCACAT TTTTTCTCAG ACAAAGATGA ATGCTCTAAG 
GATAATGGTG GATGTCAGCA CGAATGTGTC AACACGATGG GGAGCTACAT GTGTCAATGC 
CGTAATGGAT TTGTGCTACA TGACAATAAA CATGATTGCA AGGAAGCTGA GTGTGAACAG 
AAGATCCACA GTCCAAGTGG CCTCATCACC AGTCCCAACT GGCCAGACAA GTACCCAAGC 
AGGAAAGAAT GCACTTGGGA AATCAGCGCC ACTCCTGGCC ACCGAATCAA ATTAGCCTTT 
AGTGAATTTG AGATTGAGCA GCATCAAGAA TGTGCTTATG ACCACTTAGA AGTATTTGAT 
GGAGAAACAG AAAAGTCACC GATTCTTGGA CGACTGTGTG GCAACAAGAT ACCAGATCCC 
CTTGTGGCTA CTGGAAATAA AATGTTTGTT CGGTTTGTTT CTGATGCATC TGTTCAAAGA 
AAAGGCTTTC AAGCTACACA TTCTACAGAG TGTGGCGGAC GATTGAAAGC AGAATCAAAA 
CCAAGAGATC TGTACTCACA TGCTCAGTTT GGTGATAACA ACTACCCAGG ACAGGTTGAC 
TGTGAATGGC TATTAGTATC AGAACGGGGC TCTCGACTTG AATTATCCTT CCAGACATTT 
GAAGTGGAGG AAGAAGCAGA CTGTGGCTAT GACTATGTGG AGCTCTTTGA TGGTCTTGAT 
TCAACAGCTG TGGGGCTTGG TCGATTCTGT GGATCCGGGC CACCAGAAGA GATTTATTCA 
ATTGGAGATT CAGTTTTAAT TCATTTCCAC ACTGATGACA CAATCAACAA GAAGGGATTT 
CATATAAGAT ACAAAAGCAT AAGATATCCA GATACCACAC ATACCAAAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLL1 ( XM_024454195.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309963.1 Homo sapiens tolloid like 1 (TLL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu105575
Clone ID Related Accession (Same CDS sequence)	XM_024454195.1 , XM_024454194.1
Accession Version	XM_024454195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tolloid-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGA AGCGAGGGGC CCTCTACCAA CTTATAGACA GGATAAGAAG AATTGGCTTT 
GGCTTGGAGC AAAACAACAC AGTTAAGGGA AAAGTACCTC TACAATTCTC AGGGCAAAAT 
GAGAAAAATC GAGTTCCCAG AGCCGCTACA TCAAGAACGG AAAGAATATG GCCTGGAGGC 
GTTATTCCTT ATGTTATAGG AGGAAACTTC ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGGC ACTGGGAAAA GCACACATGT GTGACTTTCA TAGAAAGAAG TGATGAAGAG 
AGTTACATTG TATTCACCTA TAGGCCTTGT GGATGCTGCT CCTATGTAGG TCGGCGAGGA 
AATGGACCTC AGGCAATCTC TATCGGCAAG AACTGTGATA AATTTGGGAT TGTTGTTCAT 
GAATTGGGTC ATGTGATAGG CTTTTGGCAT GAACACACAA GACCAGATCG AGATAACCAC 
GTAACTATCA TAAGAGAAAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT GAAGATGGAG 
CCTGGAGAAG TAAACTCACT TGGAGAAAGA TATGATTTCG ACAGTATCAT GCACTATGCC 
AGGAACACCT TCTCAAGGGG GATGTTTCTG GATACCATTC TCCCCTCCCG TGATGATAAT 
GGCATACGTC CTGCAATTGG TCAGCGAACC CGTCTAAGCA AAGGAGATAT CGCACAGGCA 
AGAAAGCTGT ATAGATGTCC AGCATGTGGA GAAACTCTAC AAGAATCCAA TGGCAACCTT 
TCCTCTCCAG GATTTCCCAA TGGCTACCCT TCTTACACAC ACTGCATCTG GAGAGTTTCT 
GTGACCCCAG GGGAGAAGAT TGTTTTAAAT TTTACAACGA TGGATCTATA CAAGAGTAGT 
TTGTGCTGGT ATGACTATAT TGAAGTAAGA GACGGGTACT GGAGAAAATC ACCTCTCCTT 
GGTAGATTCT GTGGGGACAA ATTGCCTGAA GTTCTTACTT CTACAGACAG CAGAATGTGG 
ATTGAGTTTC GTAGCAGCAG TAATTGGGTA GGAAAAGGCT TTGCAGCTGT CTATGAAGCG 
ATCTGTGGAG GTGAGATACG TAAAAATGAA GGACAGATTC AGTCTCCCAA TTATCCTGAT 
GACTATCGCC CGATGAAAGA ATGTGTGTGG AAAATAACAG TGTCTGAGAG CTACCACGTC 
GGGCTGACCT TTCAGTCCTT TGAGATTGAA AGACATGACA ATTGTGCTTA TGACTACCTG 
GAAGTTAGAG ATGGAACCAG TGAAAATAGC CCTTTGATAG GGCGTTTCTG TGGTTATGAC 
AAACCTGAAG ACATAAGATC TACCTCCAAT ACTTTGTGGA TGAAGTTTGT TTCTGACGGA 
ACTGTGAACA AAGCAGGGTT TGCTGCTAAC TTTTTTAAAG AGGAAGATGA GTGTGCCAAA 
CCTGACCGTG GAGGCTGTGA GCAGCGATGT CTGAACACTC TGGGCAGTTA CCAGTGTGCC 
TGTGAGCCTG GCTATGAGCT GGGCCCAGAC AGAAGGAGCT GTGAAGCTGC TTGTGGTGGA 
CTTCTTACCA AACTTAACGG CACCATAACC ACCCCTGGCT GGCCCAAGGA GTACCCTCCT 
AATAAGAACT GTGTGTGGCA AGTGGTTGCA CCAACCCAGT ACAGAATTTC TGTGAAGTTT 
GAGTTTTTTG AATTGGAAGG CAATGAAGTT TGCAAATATG ATTATGTGGA GATCTGGAGT 
GGTCTTTCCT CTGAGTCTAA ACTGCATGGC AAATTCTGTG GCGCTGAAGT GCCTGAAGTG 
ATCACATCCC AGTTCAACAA TATGAGAATT GAATTCAAAT CTGACAATAC TGTATCCAAG 
AAGGGCTTCA AAGCACATTT TTTCTCAGAC AAAGATGAAT GCTCTAAGGA TAATGGTGGA 
TGTCAGCACG AATGTGTCAA CACGATGGGG AGCTACATGT GTCAATGCCG TAATGGATTT 
GTGCTACATG ACAATAAACA TGATTGCAAG GAAGCTGAGT GTGAACAGAA GATCCACAGT 
CCAAGTGGCC TCATCACCAG TCCCAACTGG CCAGACAAGT ACCCAAGCAG GAAAGAATGC 
ACTTGGGAAA TCAGCGCCAC TCCTGGCCAC CGAATCAAAT TAGCCTTTAG TGAATTTGAG 
ATTGAGCAGC ATCAAGAATG TGCTTATGAC CACTTAGAAG TATTTGATGG AGAAACAGAA 
AAGTCACCGA TTCTTGGACG ACTGTGTGGC AACAAGATAC CAGATCCCCT TGTGGCTACT 
GGAAATAAAA TGTTTGTTCG GTTTGTTTCT GATGCATCTG TTCAAAGAAA AGGCTTTCAA 
GCTACACATT CTACAGAGTG TGGCGGACGA TTGAAAGCAG AATCAAAACC AAGAGATCTG 
TACTCACATG CTCAGTTTGG TGATAACAAC TACCCAGGAC AGGTTGACTG TGAATGGCTA 
TTAGTATCAG AACGGGGCTC TCGACTTGAA TTATCCTTCC AGACATTTGA AGTGGAGGAA 
GAAGCAGACT GTGGCTATGA CTATGTGGAG CTCTTTGATG GTCTTGATTC AACAGCTGTG 
GGGCTTGGTC GATTCTGTGG ATCCGGGCCA CCAGAAGAGA TTTATTCAAT TGGAGATTCA 
GTTTTAATTC ATTTCCACAC TGATGACACA ATCAACAAGA AGGGATTTCA TATAAGATAC 
AAAAGCATAA GATATCCAGA TACCACACAT ACCAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309963.1
CDS	1990..4731
Translation	MSKKRGALYQLIDRIRRIGFGLEQNNTVKGKVPLQFSGQNEKNRVPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESNGNLSSPGFPNGYPSYTHCIWRVSVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKLPEVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVWKITVSESYHVGLTFQSFEIERHDNCAYDYLEVRDGTSENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVVAPTQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGAEVPEVITSQFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYMCQCRNGFVLHDNKHDCKEAECEQKIHSPSGLITSPNWPDKYPSRKECTWEISATPGHRIKLAFSEFEIEQHQECAYDHLEVFDGETEKSPILGRLCGNKIPDPLVATGNKMFVRFVSDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNNYPGQVDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVELFDGLDSTAVGLGRFCGSGPPEEIYSIGDSVLIHFHTDDTINKKGFHIRYKSIRYPDTTHTKK

Target ORF information:

RefSeq Version	XM_024454195.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tolloid like 1 (TLL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454195.1

ORF Insert Sequence:

ATGTCAAAGA AGCGAGGGGC CCTCTACCAA CTTATAGACA GGATAAGAAG AATTGGCTTT 
GGCTTGGAGC AAAACAACAC AGTTAAGGGA AAAGTACCTC TACAATTCTC AGGGCAAAAT 
GAGAAAAATC GAGTTCCCAG AGCCGCTACA TCAAGAACGG AAAGAATATG GCCTGGAGGC 
GTTATTCCTT ATGTTATAGG AGGAAACTTC ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGGC ACTGGGAAAA GCACACATGT GTGACTTTCA TAGAAAGAAG TGATGAAGAG 
AGTTACATTG TATTCACCTA TAGGCCTTGT GGATGCTGCT CCTATGTAGG TCGGCGAGGA 
AATGGACCTC AGGCAATCTC TATCGGCAAG AACTGTGATA AATTTGGGAT TGTTGTTCAT 
GAATTGGGTC ATGTGATAGG CTTTTGGCAT GAACACACAA GACCAGATCG AGATAACCAC 
GTAACTATCA TAAGAGAAAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT GAAGATGGAG 
CCTGGAGAAG TAAACTCACT TGGAGAAAGA TATGATTTCG ACAGTATCAT GCACTATGCC 
AGGAACACCT TCTCAAGGGG GATGTTTCTG GATACCATTC TCCCCTCCCG TGATGATAAT 
GGCATACGTC CTGCAATTGG TCAGCGAACC CGTCTAAGCA AAGGAGATAT CGCACAGGCA 
AGAAAGCTGT ATAGATGTCC AGCATGTGGA GAAACTCTAC AAGAATCCAA TGGCAACCTT 
TCCTCTCCAG GATTTCCCAA TGGCTACCCT TCTTACACAC ACTGCATCTG GAGAGTTTCT 
GTGACCCCAG GGGAGAAGAT TGTTTTAAAT TTTACAACGA TGGATCTATA CAAGAGTAGT 
TTGTGCTGGT ATGACTATAT TGAAGTAAGA GACGGGTACT GGAGAAAATC ACCTCTCCTT 
GGTAGATTCT GTGGGGACAA ATTGCCTGAA GTTCTTACTT CTACAGACAG CAGAATGTGG 
ATTGAGTTTC GTAGCAGCAG TAATTGGGTA GGAAAAGGCT TTGCAGCTGT CTATGAAGCG 
ATCTGTGGAG GTGAGATACG TAAAAATGAA GGACAGATTC AGTCTCCCAA TTATCCTGAT 
GACTATCGCC CGATGAAAGA ATGTGTGTGG AAAATAACAG TGTCTGAGAG CTACCACGTC 
GGGCTGACCT TTCAGTCCTT TGAGATTGAA AGACATGACA ATTGTGCTTA TGACTACCTG 
GAAGTTAGAG ATGGAACCAG TGAAAATAGC CCTTTGATAG GGCGTTTCTG TGGTTATGAC 
AAACCTGAAG ACATAAGATC TACCTCCAAT ACTTTGTGGA TGAAGTTTGT TTCTGACGGA 
ACTGTGAACA AAGCAGGGTT TGCTGCTAAC TTTTTTAAAG AGGAAGATGA GTGTGCCAAA 
CCTGACCGTG GAGGCTGTGA GCAGCGATGT CTGAACACTC TGGGCAGTTA CCAGTGTGCC 
TGTGAGCCTG GCTATGAGCT GGGCCCAGAC AGAAGGAGCT GTGAAGCTGC TTGTGGTGGA 
CTTCTTACCA AACTTAACGG CACCATAACC ACCCCTGGCT GGCCCAAGGA GTACCCTCCT 
AATAAGAACT GTGTGTGGCA AGTGGTTGCA CCAACCCAGT ACAGAATTTC TGTGAAGTTT 
GAGTTTTTTG AATTGGAAGG CAATGAAGTT TGCAAATATG ATTATGTGGA GATCTGGAGT 
GGTCTTTCCT CTGAGTCTAA ACTGCATGGC AAATTCTGTG GCGCTGAAGT GCCTGAAGTG 
ATCACATCCC AGTTCAACAA TATGAGAATT GAATTCAAAT CTGACAATAC TGTATCCAAG 
AAGGGCTTCA AAGCACATTT TTTCTCAGAC AAAGATGAAT GCTCTAAGGA TAATGGTGGA 
TGTCAGCACG AATGTGTCAA CACGATGGGG AGCTACATGT GTCAATGCCG TAATGGATTT 
GTGCTACATG ACAATAAACA TGATTGCAAG GAAGCTGAGT GTGAACAGAA GATCCACAGT 
CCAAGTGGCC TCATCACCAG TCCCAACTGG CCAGACAAGT ACCCAAGCAG GAAAGAATGC 
ACTTGGGAAA TCAGCGCCAC TCCTGGCCAC CGAATCAAAT TAGCCTTTAG TGAATTTGAG 
ATTGAGCAGC ATCAAGAATG TGCTTATGAC CACTTAGAAG TATTTGATGG AGAAACAGAA 
AAGTCACCGA TTCTTGGACG ACTGTGTGGC AACAAGATAC CAGATCCCCT TGTGGCTACT 
GGAAATAAAA TGTTTGTTCG GTTTGTTTCT GATGCATCTG TTCAAAGAAA AGGCTTTCAA 
GCTACACATT CTACAGAGTG TGGCGGACGA TTGAAAGCAG AATCAAAACC AAGAGATCTG 
TACTCACATG CTCAGTTTGG TGATAACAAC TACCCAGGAC AGGTTGACTG TGAATGGCTA 
TTAGTATCAG AACGGGGCTC TCGACTTGAA TTATCCTTCC AGACATTTGA AGTGGAGGAA 
GAAGCAGACT GTGGCTATGA CTATGTGGAG CTCTTTGATG GTCTTGATTC AACAGCTGTG 
GGGCTTGGTC GATTCTGTGG ATCCGGGCCA CCAGAAGAGA TTTATTCAAT TGGAGATTCA 
GTTTTAATTC ATTTCCACAC TGATGACACA ATCAACAAGA AGGGATTTCA TATAAGATAC 
AAAAGCATAA GATATCCAGA TACCACACAT ACCAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLL1 ( XM_024454194.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309962.1 Homo sapiens tolloid like 1 (TLL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu105575
Clone ID Related Accession (Same CDS sequence)	XM_024454195.1 , XM_024454194.1
Accession Version	XM_024454194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tolloid-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGA AGCGAGGGGC CCTCTACCAA CTTATAGACA GGATAAGAAG AATTGGCTTT 
GGCTTGGAGC AAAACAACAC AGTTAAGGGA AAAGTACCTC TACAATTCTC AGGGCAAAAT 
GAGAAAAATC GAGTTCCCAG AGCCGCTACA TCAAGAACGG AAAGAATATG GCCTGGAGGC 
GTTATTCCTT ATGTTATAGG AGGAAACTTC ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGGC ACTGGGAAAA GCACACATGT GTGACTTTCA TAGAAAGAAG TGATGAAGAG 
AGTTACATTG TATTCACCTA TAGGCCTTGT GGATGCTGCT CCTATGTAGG TCGGCGAGGA 
AATGGACCTC AGGCAATCTC TATCGGCAAG AACTGTGATA AATTTGGGAT TGTTGTTCAT 
GAATTGGGTC ATGTGATAGG CTTTTGGCAT GAACACACAA GACCAGATCG AGATAACCAC 
GTAACTATCA TAAGAGAAAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT GAAGATGGAG 
CCTGGAGAAG TAAACTCACT TGGAGAAAGA TATGATTTCG ACAGTATCAT GCACTATGCC 
AGGAACACCT TCTCAAGGGG GATGTTTCTG GATACCATTC TCCCCTCCCG TGATGATAAT 
GGCATACGTC CTGCAATTGG TCAGCGAACC CGTCTAAGCA AAGGAGATAT CGCACAGGCA 
AGAAAGCTGT ATAGATGTCC AGCATGTGGA GAAACTCTAC AAGAATCCAA TGGCAACCTT 
TCCTCTCCAG GATTTCCCAA TGGCTACCCT TCTTACACAC ACTGCATCTG GAGAGTTTCT 
GTGACCCCAG GGGAGAAGAT TGTTTTAAAT TTTACAACGA TGGATCTATA CAAGAGTAGT 
TTGTGCTGGT ATGACTATAT TGAAGTAAGA GACGGGTACT GGAGAAAATC ACCTCTCCTT 
GGTAGATTCT GTGGGGACAA ATTGCCTGAA GTTCTTACTT CTACAGACAG CAGAATGTGG 
ATTGAGTTTC GTAGCAGCAG TAATTGGGTA GGAAAAGGCT TTGCAGCTGT CTATGAAGCG 
ATCTGTGGAG GTGAGATACG TAAAAATGAA GGACAGATTC AGTCTCCCAA TTATCCTGAT 
GACTATCGCC CGATGAAAGA ATGTGTGTGG AAAATAACAG TGTCTGAGAG CTACCACGTC 
GGGCTGACCT TTCAGTCCTT TGAGATTGAA AGACATGACA ATTGTGCTTA TGACTACCTG 
GAAGTTAGAG ATGGAACCAG TGAAAATAGC CCTTTGATAG GGCGTTTCTG TGGTTATGAC 
AAACCTGAAG ACATAAGATC TACCTCCAAT ACTTTGTGGA TGAAGTTTGT TTCTGACGGA 
ACTGTGAACA AAGCAGGGTT TGCTGCTAAC TTTTTTAAAG AGGAAGATGA GTGTGCCAAA 
CCTGACCGTG GAGGCTGTGA GCAGCGATGT CTGAACACTC TGGGCAGTTA CCAGTGTGCC 
TGTGAGCCTG GCTATGAGCT GGGCCCAGAC AGAAGGAGCT GTGAAGCTGC TTGTGGTGGA 
CTTCTTACCA AACTTAACGG CACCATAACC ACCCCTGGCT GGCCCAAGGA GTACCCTCCT 
AATAAGAACT GTGTGTGGCA AGTGGTTGCA CCAACCCAGT ACAGAATTTC TGTGAAGTTT 
GAGTTTTTTG AATTGGAAGG CAATGAAGTT TGCAAATATG ATTATGTGGA GATCTGGAGT 
GGTCTTTCCT CTGAGTCTAA ACTGCATGGC AAATTCTGTG GCGCTGAAGT GCCTGAAGTG 
ATCACATCCC AGTTCAACAA TATGAGAATT GAATTCAAAT CTGACAATAC TGTATCCAAG 
AAGGGCTTCA AAGCACATTT TTTCTCAGAC AAAGATGAAT GCTCTAAGGA TAATGGTGGA 
TGTCAGCACG AATGTGTCAA CACGATGGGG AGCTACATGT GTCAATGCCG TAATGGATTT 
GTGCTACATG ACAATAAACA TGATTGCAAG GAAGCTGAGT GTGAACAGAA GATCCACAGT 
CCAAGTGGCC TCATCACCAG TCCCAACTGG CCAGACAAGT ACCCAAGCAG GAAAGAATGC 
ACTTGGGAAA TCAGCGCCAC TCCTGGCCAC CGAATCAAAT TAGCCTTTAG TGAATTTGAG 
ATTGAGCAGC ATCAAGAATG TGCTTATGAC CACTTAGAAG TATTTGATGG AGAAACAGAA 
AAGTCACCGA TTCTTGGACG ACTGTGTGGC AACAAGATAC CAGATCCCCT TGTGGCTACT 
GGAAATAAAA TGTTTGTTCG GTTTGTTTCT GATGCATCTG TTCAAAGAAA AGGCTTTCAA 
GCTACACATT CTACAGAGTG TGGCGGACGA TTGAAAGCAG AATCAAAACC AAGAGATCTG 
TACTCACATG CTCAGTTTGG TGATAACAAC TACCCAGGAC AGGTTGACTG TGAATGGCTA 
TTAGTATCAG AACGGGGCTC TCGACTTGAA TTATCCTTCC AGACATTTGA AGTGGAGGAA 
GAAGCAGACT GTGGCTATGA CTATGTGGAG CTCTTTGATG GTCTTGATTC AACAGCTGTG 
GGGCTTGGTC GATTCTGTGG ATCCGGGCCA CCAGAAGAGA TTTATTCAAT TGGAGATTCA 
GTTTTAATTC ATTTCCACAC TGATGACACA ATCAACAAGA AGGGATTTCA TATAAGATAC 
AAAAGCATAA GATATCCAGA TACCACACAT ACCAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309962.1
CDS	151..2892
Translation	MSKKRGALYQLIDRIRRIGFGLEQNNTVKGKVPLQFSGQNEKNRVPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESNGNLSSPGFPNGYPSYTHCIWRVSVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKLPEVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVWKITVSESYHVGLTFQSFEIERHDNCAYDYLEVRDGTSENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVVAPTQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGAEVPEVITSQFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYMCQCRNGFVLHDNKHDCKEAECEQKIHSPSGLITSPNWPDKYPSRKECTWEISATPGHRIKLAFSEFEIEQHQECAYDHLEVFDGETEKSPILGRLCGNKIPDPLVATGNKMFVRFVSDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNNYPGQVDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVELFDGLDSTAVGLGRFCGSGPPEEIYSIGDSVLIHFHTDDTINKKGFHIRYKSIRYPDTTHTKK

Target ORF information:

RefSeq Version	XM_024454194.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tolloid like 1 (TLL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454194.1

ORF Insert Sequence:

ATGTCAAAGA AGCGAGGGGC CCTCTACCAA CTTATAGACA GGATAAGAAG AATTGGCTTT 
GGCTTGGAGC AAAACAACAC AGTTAAGGGA AAAGTACCTC TACAATTCTC AGGGCAAAAT 
GAGAAAAATC GAGTTCCCAG AGCCGCTACA TCAAGAACGG AAAGAATATG GCCTGGAGGC 
GTTATTCCTT ATGTTATAGG AGGAAACTTC ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGGC ACTGGGAAAA GCACACATGT GTGACTTTCA TAGAAAGAAG TGATGAAGAG 
AGTTACATTG TATTCACCTA TAGGCCTTGT GGATGCTGCT CCTATGTAGG TCGGCGAGGA 
AATGGACCTC AGGCAATCTC TATCGGCAAG AACTGTGATA AATTTGGGAT TGTTGTTCAT 
GAATTGGGTC ATGTGATAGG CTTTTGGCAT GAACACACAA GACCAGATCG AGATAACCAC 
GTAACTATCA TAAGAGAAAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT GAAGATGGAG 
CCTGGAGAAG TAAACTCACT TGGAGAAAGA TATGATTTCG ACAGTATCAT GCACTATGCC 
AGGAACACCT TCTCAAGGGG GATGTTTCTG GATACCATTC TCCCCTCCCG TGATGATAAT 
GGCATACGTC CTGCAATTGG TCAGCGAACC CGTCTAAGCA AAGGAGATAT CGCACAGGCA 
AGAAAGCTGT ATAGATGTCC AGCATGTGGA GAAACTCTAC AAGAATCCAA TGGCAACCTT 
TCCTCTCCAG GATTTCCCAA TGGCTACCCT TCTTACACAC ACTGCATCTG GAGAGTTTCT 
GTGACCCCAG GGGAGAAGAT TGTTTTAAAT TTTACAACGA TGGATCTATA CAAGAGTAGT 
TTGTGCTGGT ATGACTATAT TGAAGTAAGA GACGGGTACT GGAGAAAATC ACCTCTCCTT 
GGTAGATTCT GTGGGGACAA ATTGCCTGAA GTTCTTACTT CTACAGACAG CAGAATGTGG 
ATTGAGTTTC GTAGCAGCAG TAATTGGGTA GGAAAAGGCT TTGCAGCTGT CTATGAAGCG 
ATCTGTGGAG GTGAGATACG TAAAAATGAA GGACAGATTC AGTCTCCCAA TTATCCTGAT 
GACTATCGCC CGATGAAAGA ATGTGTGTGG AAAATAACAG TGTCTGAGAG CTACCACGTC 
GGGCTGACCT TTCAGTCCTT TGAGATTGAA AGACATGACA ATTGTGCTTA TGACTACCTG 
GAAGTTAGAG ATGGAACCAG TGAAAATAGC CCTTTGATAG GGCGTTTCTG TGGTTATGAC 
AAACCTGAAG ACATAAGATC TACCTCCAAT ACTTTGTGGA TGAAGTTTGT TTCTGACGGA 
ACTGTGAACA AAGCAGGGTT TGCTGCTAAC TTTTTTAAAG AGGAAGATGA GTGTGCCAAA 
CCTGACCGTG GAGGCTGTGA GCAGCGATGT CTGAACACTC TGGGCAGTTA CCAGTGTGCC 
TGTGAGCCTG GCTATGAGCT GGGCCCAGAC AGAAGGAGCT GTGAAGCTGC TTGTGGTGGA 
CTTCTTACCA AACTTAACGG CACCATAACC ACCCCTGGCT GGCCCAAGGA GTACCCTCCT 
AATAAGAACT GTGTGTGGCA AGTGGTTGCA CCAACCCAGT ACAGAATTTC TGTGAAGTTT 
GAGTTTTTTG AATTGGAAGG CAATGAAGTT TGCAAATATG ATTATGTGGA GATCTGGAGT 
GGTCTTTCCT CTGAGTCTAA ACTGCATGGC AAATTCTGTG GCGCTGAAGT GCCTGAAGTG 
ATCACATCCC AGTTCAACAA TATGAGAATT GAATTCAAAT CTGACAATAC TGTATCCAAG 
AAGGGCTTCA AAGCACATTT TTTCTCAGAC AAAGATGAAT GCTCTAAGGA TAATGGTGGA 
TGTCAGCACG AATGTGTCAA CACGATGGGG AGCTACATGT GTCAATGCCG TAATGGATTT 
GTGCTACATG ACAATAAACA TGATTGCAAG GAAGCTGAGT GTGAACAGAA GATCCACAGT 
CCAAGTGGCC TCATCACCAG TCCCAACTGG CCAGACAAGT ACCCAAGCAG GAAAGAATGC 
ACTTGGGAAA TCAGCGCCAC TCCTGGCCAC CGAATCAAAT TAGCCTTTAG TGAATTTGAG 
ATTGAGCAGC ATCAAGAATG TGCTTATGAC CACTTAGAAG TATTTGATGG AGAAACAGAA 
AAGTCACCGA TTCTTGGACG ACTGTGTGGC AACAAGATAC CAGATCCCCT TGTGGCTACT 
GGAAATAAAA TGTTTGTTCG GTTTGTTTCT GATGCATCTG TTCAAAGAAA AGGCTTTCAA 
GCTACACATT CTACAGAGTG TGGCGGACGA TTGAAAGCAG AATCAAAACC AAGAGATCTG 
TACTCACATG CTCAGTTTGG TGATAACAAC TACCCAGGAC AGGTTGACTG TGAATGGCTA 
TTAGTATCAG AACGGGGCTC TCGACTTGAA TTATCCTTCC AGACATTTGA AGTGGAGGAA 
GAAGCAGACT GTGGCTATGA CTATGTGGAG CTCTTTGATG GTCTTGATTC AACAGCTGTG 
GGGCTTGGTC GATTCTGTGG ATCCGGGCCA CCAGAAGAGA TTTATTCAAT TGGAGATTCA 
GTTTTAATTC ATTTCCACAC TGATGACACA ATCAACAAGA AGGGATTTCA TATAAGATAC 
AAAAGCATAA GATATCCAGA TACCACACAT ACCAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLL1 ( XM_017008570.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864059.1 Homo sapiens tolloid like 1 (TLL1), transcript variant X1, mRNA.

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CloneID	OHu95384
Clone ID Related Accession (Same CDS sequence)	XM_017008570.1
Accession Version	XM_017008570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tolloid-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGGAG GTCAGAGTCA ATCTGTATTT TGGGGCGATA TTGCCTTAGA TGATGAAGAC 
TTAAATATCT TTCAAATAGA TAGGACAATT GACCTTACGC AGAACCCCTT TGGAAACCTT 
GGACATACCA CAGGTGGACT TGGAGACCAT GCTATGTCAA AGAAGCGAGG GGCCCTCTAC 
CAACTTATAG ACAGGATAAG AAGAATTGGC TTTGGCTTGG AGCAAAACAA CACAGTTAAG 
GGAAAAGTAC CTCTACAATT CTCAGGGCAA AATGAGAAAA ATCGAGTTCC CAGAGCCGCT 
ACATCAAGAA CGGAAAGAAT ATGGCCTGGA GGCGTTATTC CTTATGTTAT AGGAGGAAAC 
TTCACTGGCA GCCAGAGAGC CATGTTCAAG CAGGCCATGA GGCACTGGGA AAAGCACACA 
TGTGTGACTT TCATAGAAAG AAGTGATGAA GAGAGTTACA TTGTATTCAC CTATAGGCCT 
TGTGGATGCT GCTCCTATGT AGGTCGGCGA GGAAATGGAC CTCAGGCAAT CTCTATCGGC 
AAGAACTGTG ATAAATTTGG GATTGTTGTT CATGAATTGG GTCATGTGAT AGGCTTTTGG 
CATGAACACA CAAGACCAGA TCGAGATAAC CACGTAACTA TCATAAGAGA AAACATCCAG 
CCAGGTCAAG AGTACAATTT TCTGAAGATG GAGCCTGGAG AAGTAAACTC ACTTGGAGAA 
AGATATGATT TCGACAGTAT CATGCACTAT GCCAGGAACA CCTTCTCAAG GGGGATGTTT 
CTGGATACCA TTCTCCCCTC CCGTGATGAT AATGGCATAC GTCCTGCAAT TGGTCAGCGA 
ACCCGTCTAA GCAAAGGAGA TATCGCACAG GCAAGAAAGC TGTATAGATG TCCAGCATGT 
GGAGAAACTC TACAAGAATC CAATGGCAAC CTTTCCTCTC CAGGATTTCC CAATGGCTAC 
CCTTCTTACA CACACTGCAT CTGGAGAGTT TCTGTGACCC CAGGGGAGAA GATTGTTTTA 
AATTTTACAA CGATGGATCT ATACAAGAGT AGTTTGTGCT GGTATGACTA TATTGAAGTA 
AGAGACGGGT ACTGGAGAAA ATCACCTCTC CTTGGTAGAT TCTGTGGGGA CAAATTGCCT 
GAAGTTCTTA CTTCTACAGA CAGCAGAATG TGGATTGAGT TTCGTAGCAG CAGTAATTGG 
GTAGGAAAAG GCTTTGCAGC TGTCTATGAA GCGATCTGTG GAGGTGAGAT ACGTAAAAAT 
GAAGGACAGA TTCAGTCTCC CAATTATCCT GATGACTATC GCCCGATGAA AGAATGTGTG 
TGGAAAATAA CAGTGTCTGA GAGCTACCAC GTCGGGCTGA CCTTTCAGTC CTTTGAGATT 
GAAAGACATG ACAATTGTGC TTATGACTAC CTGGAAGTTA GAGATGGAAC CAGTGAAAAT 
AGCCCTTTGA TAGGGCGTTT CTGTGGTTAT GACAAACCTG AAGACATAAG ATCTACCTCC 
AATACTTTGT GGATGAAGTT TGTTTCTGAC GGAACTGTGA ACAAAGCAGG GTTTGCTGCT 
AACTTTTTTA AAGAGGAAGA TGAGTGTGCC AAACCTGACC GTGGAGGCTG TGAGCAGCGA 
TGTCTGAACA CTCTGGGCAG TTACCAGTGT GCCTGTGAGC CTGGCTATGA GCTGGGCCCA 
GACAGAAGGA GCTGTGAAGC TGCTTGTGGT GGACTTCTTA CCAAACTTAA CGGCACCATA 
ACCACCCCTG GCTGGCCCAA GGAGTACCCT CCTAATAAGA ACTGTGTGTG GCAAGTGGTT 
GCACCAACCC AGTACAGAAT TTCTGTGAAG TTTGAGTTTT TTGAATTGGA AGGCAATGAA 
GTTTGCAAAT ATGATTATGT GGAGATCTGG AGTGGTCTTT CCTCTGAGTC TAAACTGCAT 
GGCAAATTCT GTGGCGCTGA AGTGCCTGAA GTGATCACAT CCCAGTTCAA CAATATGAGA 
ATTGAATTCA AATCTGACAA TACTGTATCC AAGAAGGGCT TCAAAGCACA TTTTTTCTCA 
GACAAAGATG AATGCTCTAA GGATAATGGT GGATGTCAGC ACGAATGTGT CAACACGATG 
GGGAGCTACA TGTGTCAATG CCGTAATGGA TTTGTGCTAC ATGACAATAA ACATGATTGC 
AAGGAAGCTG AGTGTGAACA GAAGATCCAC AGTCCAAGTG GCCTCATCAC CAGTCCCAAC 
TGGCCAGACA AGTACCCAAG CAGGAAAGAA TGCACTTGGG AAATCAGCGC CACTCCTGGC 
CACCGAATCA AATTAGCCTT TAGTGAATTT GAGATTGAGC AGCATCAAGA ATGTGCTTAT 
GACCACTTAG AAGTATTTGA TGGAGAAACA GAAAAGTCAC CGATTCTTGG ACGACTGTGT 
GGCAACAAGA TACCAGATCC CCTTGTGGCT ACTGGAAATA AAATGTTTGT TCGGTTTGTT 
TCTGATGCAT CTGTTCAAAG AAAAGGCTTT CAAGCTACAC ATTCTACAGA GTGTGGCGGA 
CGATTGAAAG CAGAATCAAA ACCAAGAGAT CTGTACTCAC ATGCTCAGTT TGGTGATAAC 
AACTACCCAG GACAGGTTGA CTGTGAATGG CTATTAGTAT CAGAACGGGG CTCTCGACTT 
GAATTATCCT TCCAGACATT TGAAGTGGAG GAAGAAGCAG ACTGTGGCTA TGACTATGTG 
GAGCTCTTTG ATGGTCTTGA TTCAACAGCT GTGGGGCTTG GTCGATTCTG TGGATCCGGG 
CCACCAGAAG AGATTTATTC AATTGGAGAT TCAGTTTTAA TTCATTTCCA CACTGATGAC 
ACAATCAACA AGAAGGGATT TCATATAAGA TACAAAAGCA TAAGATATCC AGATACCACA 
CATACCAAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864059.1
CDS	921..3815
Translation	MRGGQSQSVFWGDIALDDEDLNIFQIDRTIDLTQNPFGNLGHTTGGLGDHAMSKKRGALYQLIDRIRRIGFGLEQNNTVKGKVPLQFSGQNEKNRVPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESNGNLSSPGFPNGYPSYTHCIWRVSVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKLPEVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVWKITVSESYHVGLTFQSFEIERHDNCAYDYLEVRDGTSENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVVAPTQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGAEVPEVITSQFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYMCQCRNGFVLHDNKHDCKEAECEQKIHSPSGLITSPNWPDKYPSRKECTWEISATPGHRIKLAFSEFEIEQHQECAYDHLEVFDGETEKSPILGRLCGNKIPDPLVATGNKMFVRFVSDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNNYPGQVDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVELFDGLDSTAVGLGRFCGSGPPEEIYSIGDSVLIHFHTDDTINKKGFHIRYKSIRYPDTTHTKK

Target ORF information:

RefSeq Version	XM_017008570.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tolloid like 1 (TLL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008570.1

ORF Insert Sequence:

ATGAGGGGAG GTCAGAGTCA ATCTGTATTT TGGGGCGATA TTGCCTTAGA TGATGAAGAC 
TTAAATATCT TTCAAATAGA TAGGACAATT GACCTTACGC AGAACCCCTT TGGAAACCTT 
GGACATACCA CAGGTGGACT TGGAGACCAT GCTATGTCAA AGAAGCGAGG GGCCCTCTAC 
CAACTTATAG ACAGGATAAG AAGAATTGGC TTTGGCTTGG AGCAAAACAA CACAGTTAAG 
GGAAAAGTAC CTCTACAATT CTCAGGGCAA AATGAGAAAA ATCGAGTTCC CAGAGCCGCT 
ACATCAAGAA CGGAAAGAAT ATGGCCTGGA GGCGTTATTC CTTATGTTAT AGGAGGAAAC 
TTCACTGGCA GCCAGAGAGC CATGTTCAAG CAGGCCATGA GGCACTGGGA AAAGCACACA 
TGTGTGACTT TCATAGAAAG AAGTGATGAA GAGAGTTACA TTGTATTCAC CTATAGGCCT 
TGTGGATGCT GCTCCTATGT AGGTCGGCGA GGAAATGGAC CTCAGGCAAT CTCTATCGGC 
AAGAACTGTG ATAAATTTGG GATTGTTGTT CATGAATTGG GTCATGTGAT AGGCTTTTGG 
CATGAACACA CAAGACCAGA TCGAGATAAC CACGTAACTA TCATAAGAGA AAACATCCAG 
CCAGGTCAAG AGTACAATTT TCTGAAGATG GAGCCTGGAG AAGTAAACTC ACTTGGAGAA 
AGATATGATT TCGACAGTAT CATGCACTAT GCCAGGAACA CCTTCTCAAG GGGGATGTTT 
CTGGATACCA TTCTCCCCTC CCGTGATGAT AATGGCATAC GTCCTGCAAT TGGTCAGCGA 
ACCCGTCTAA GCAAAGGAGA TATCGCACAG GCAAGAAAGC TGTATAGATG TCCAGCATGT 
GGAGAAACTC TACAAGAATC CAATGGCAAC CTTTCCTCTC CAGGATTTCC CAATGGCTAC 
CCTTCTTACA CACACTGCAT CTGGAGAGTT TCTGTGACCC CAGGGGAGAA GATTGTTTTA 
AATTTTACAA CGATGGATCT ATACAAGAGT AGTTTGTGCT GGTATGACTA TATTGAAGTA 
AGAGACGGGT ACTGGAGAAA ATCACCTCTC CTTGGTAGAT TCTGTGGGGA CAAATTGCCT 
GAAGTTCTTA CTTCTACAGA CAGCAGAATG TGGATTGAGT TTCGTAGCAG CAGTAATTGG 
GTAGGAAAAG GCTTTGCAGC TGTCTATGAA GCGATCTGTG GAGGTGAGAT ACGTAAAAAT 
GAAGGACAGA TTCAGTCTCC CAATTATCCT GATGACTATC GCCCGATGAA AGAATGTGTG 
TGGAAAATAA CAGTGTCTGA GAGCTACCAC GTCGGGCTGA CCTTTCAGTC CTTTGAGATT 
GAAAGACATG ACAATTGTGC TTATGACTAC CTGGAAGTTA GAGATGGAAC CAGTGAAAAT 
AGCCCTTTGA TAGGGCGTTT CTGTGGTTAT GACAAACCTG AAGACATAAG ATCTACCTCC 
AATACTTTGT GGATGAAGTT TGTTTCTGAC GGAACTGTGA ACAAAGCAGG GTTTGCTGCT 
AACTTTTTTA AAGAGGAAGA TGAGTGTGCC AAACCTGACC GTGGAGGCTG TGAGCAGCGA 
TGTCTGAACA CTCTGGGCAG TTACCAGTGT GCCTGTGAGC CTGGCTATGA GCTGGGCCCA 
GACAGAAGGA GCTGTGAAGC TGCTTGTGGT GGACTTCTTA CCAAACTTAA CGGCACCATA 
ACCACCCCTG GCTGGCCCAA GGAGTACCCT CCTAATAAGA ACTGTGTGTG GCAAGTGGTT 
GCACCAACCC AGTACAGAAT TTCTGTGAAG TTTGAGTTTT TTGAATTGGA AGGCAATGAA 
GTTTGCAAAT ATGATTATGT GGAGATCTGG AGTGGTCTTT CCTCTGAGTC TAAACTGCAT 
GGCAAATTCT GTGGCGCTGA AGTGCCTGAA GTGATCACAT CCCAGTTCAA CAATATGAGA 
ATTGAATTCA AATCTGACAA TACTGTATCC AAGAAGGGCT TCAAAGCACA TTTTTTCTCA 
GACAAAGATG AATGCTCTAA GGATAATGGT GGATGTCAGC ACGAATGTGT CAACACGATG 
GGGAGCTACA TGTGTCAATG CCGTAATGGA TTTGTGCTAC ATGACAATAA ACATGATTGC 
AAGGAAGCTG AGTGTGAACA GAAGATCCAC AGTCCAAGTG GCCTCATCAC CAGTCCCAAC 
TGGCCAGACA AGTACCCAAG CAGGAAAGAA TGCACTTGGG AAATCAGCGC CACTCCTGGC 
CACCGAATCA AATTAGCCTT TAGTGAATTT GAGATTGAGC AGCATCAAGA ATGTGCTTAT 
GACCACTTAG AAGTATTTGA TGGAGAAACA GAAAAGTCAC CGATTCTTGG ACGACTGTGT 
GGCAACAAGA TACCAGATCC CCTTGTGGCT ACTGGAAATA AAATGTTTGT TCGGTTTGTT 
TCTGATGCAT CTGTTCAAAG AAAAGGCTTT CAAGCTACAC ATTCTACAGA GTGTGGCGGA 
CGATTGAAAG CAGAATCAAA ACCAAGAGAT CTGTACTCAC ATGCTCAGTT TGGTGATAAC 
AACTACCCAG GACAGGTTGA CTGTGAATGG CTATTAGTAT CAGAACGGGG CTCTCGACTT 
GAATTATCCT TCCAGACATT TGAAGTGGAG GAAGAAGCAG ACTGTGGCTA TGACTATGTG 
GAGCTCTTTG ATGGTCTTGA TTCAACAGCT GTGGGGCTTG GTCGATTCTG TGGATCCGGG 
CCACCAGAAG AGATTTATTC AATTGGAGAT TCAGTTTTAA TTCATTTCCA CACTGATGAC 
ACAATCAACA AGAAGGGATT TCATATAAGA TACAAAAGCA TAAGATATCC AGATACCACA 
CATACCAAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Combination of PNPLA3 and TLL1 polymorphism can predict advanced fibrosis in Japanese patients with nonalcoholic fatty liver disease.
Journal of gastroenterology(2017 Jul)
Seko Y,Yamaguchi K,Mizuno N,Okuda K,Takemura M,Taketani H,Hara T,Umemura A,Nishikawa T,Moriguchi M,Yasui K,Kamaguchi M,Nishioji K,Mochizuki N,Kobayashi M,Mori K,Tanaka S,Matsuura K,Tanaka Y,Itoh Y

Genome-Wide Association Study Identifies TLL1 Variant Associated With Development of Hepatocellular Carcinoma After Eradication of Hepatitis C Virus Infection.
Gastroenterology152(6)1383-1394(2017 May)
Matsuura K,Sawai H,Ikeo K,Ogawa S,Iio E,Isogawa M,Shimada N,Komori A,Toyoda H,Kumada T,Namisaki T,Yoshiji H,Sakamoto N,Nakagawa M,Asahina Y,Kurosaki M,Izumi N,Enomoto N,Kusakabe A,Kajiwara E,Itoh Y,Ide T,Tamori A,Matsubara M,Kawada N,Shirabe K,Tomita E,Honda M,Kaneko S,Nishina S,Suetsugu A,Hiasa Y,Watanabe H,Genda T,Sakaida I,Nishiguchi S,Takaguchi K,Tanaka E,Sugihara J,Shimada M,Kondo Y,Kawai Y,Kojima K,Nagasaki M,Tokunaga K,Tanaka Y,

Association of TLL1 gene polymorphism (rs1503298, T > C) with coronary heart disease in PREDICT, UDACS and ED cohorts.
Journal of the College of Physicians and Surgeons--Pakistan : JCPSP24(9)615-9(2014 Sep)
Zain M,Awan FR,Cooper JA,Li KW,Palmen J,Acharya J,Howard P,Baig SM,Elkeles RS,Stephens JW,Ireland H,Humphries SE

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TLL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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