PAPPA2 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_020318.2	NP_064714.2	pappalysin-2 isoform 1 precursor
XM_017002025.2	XP_016857514.1	pappalysin-2 isoform X1
NM_020318.3	NP_064714.2	pappalysin-2 isoform 1 precursor
XM_011509857.1	XP_011508159.1	pappalysin-2 isoform X1
NM_021936.2	NP_068755.2	pappalysin-2 isoform 2 precursor
XM_017002024.1	XP_016857513.1	pappalysin-2 isoform X1
XM_017002025.1	XP_016857514.1	pappalysin-2 isoform X1
NM_021936.3	NP_068755.2	pappalysin-2 isoform 2 precursor
XM_011509858.2	XP_011508160.1	pappalysin-2 isoform X1
XM_017002023.1	XP_016857512.1	pappalysin-2 isoform X1
XM_005245422.3	XP_005245479.1	pappalysin-2 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18995	NM_020318.3 Latest version!	Homo sapiens pappalysin 2 (PAPPA2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu18995	XM_011509858.2 Latest version!	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu18995	XM_017002025.2 Latest version!	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu18995	XM_011509857.1 Latest version!	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu18995	XM_017002024.1 Latest version!	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu18995	XM_005245422.3 Latest version!	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu18995	XM_017002023.1 Latest version!	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu29954	NM_021936.3 Latest version!	Homo sapiens pappalysin 2 (PAPPA2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu18995	NM_020318.2	Homo sapiens pappalysin 2 (PAPPA2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu18995	XM_017002025.1	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu29954	NM_021936.2	Homo sapiens pappalysin 2 (PAPPA2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

PAPPA2 ( NM_020318.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_064714.2 Homo sapiens pappalysin 2 (PAPPA2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18995
Clone ID Related Accession (Same CDS sequence)	NM_020318.2 , XM_017002025.2 , NM_020318.3 , XM_011509857.1 , XM_017002024.1 , XM_017002025.1 , XM_011509858.2 , XM_017002023.1 , XM_005245422.3
Accession Version	NM_020318.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-26
Organism	Homo sapiens(human)
Product	pappalysin-2 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC062665.1, AF342989.1 and AF311940.1. This sequence is a reference standard in the RefSeqGene project. On Oct 14, 2006 this sequence version replaced NM_020318.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF342989.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END##

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064714.2
CDS	1165..6540
Translation	MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPRASPQHHLFGVYPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRGAVEEPAAPWVGDSPIGQSELLGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTLNEPKPETQRRGWAKSRQRRQVWKRRAEDGQGDSGISSHFQPWPKHSLKHRVKKSPPEESNQNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLAEIPREAFTVEAWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKATILISHSRYQPGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFRGHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEVLQGFEPEPEILSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNPIVSEEQIRLQHEALNEAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPQVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAATWPWDKDAVTHLGGIVLSPAYYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDLCADTAPTPKSELCREPEPTSDTCGFTRFPGAPFTNYMSYTDDNCTDNFTPNQVARMHCYLDLVYQQWTESRKPTPIPIPPMVIGQTNKSLTIHWLPPISGVVYDRASGSLCGACTEDGTFRQYVHTASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPEVHLYHMNMTVPCPTEGCSLELLFQHPVQADTLTLWVTSFFMESSQVLFDTEILLENKESVHLGPLDTFCDIPLTIKLHVDGKVSGVKVYTFDERIEIDAALLTSQPHSPLCSGCRPVRYQVLRDPPFASGLPVVVTHSHRKFTDVEVTPGQMYQYQVLAEAGGELGEASPPLNHIHGAPYCGDGKVSERLGEECDDGDLVSGDGCSKVCELEEGFNCVGEPSLCYMYEGDGICEPFERKTSIVDCGIYTPKGYLDQWATRAYSSHEDKKKCPVSLVTGEPHSLICTSYHPDLPNHRPLTGWFPCVASENETQDDRSEQPEGSLKKEDEVWLKVCFNRPGEARAIFIFLTTDGLVPGEHQQPTVTLYLTDVRGSNHSLGTYGLSCQHNPLIINVTHHQNVLFHHTTSVLLNFSSPRVGISAVALRTSSRIGLSAPSNCISEDEGQNHQGQSCIHRPCGKQDSCPSLLLDHADVVNCTSIGPGLMKCAITCQRGFALQASSGQYIRPMQKEILLTCSSGHWDQNVSCLPVDCGVPDPSLVNYANFSCSEGTKFLKRCSISCVPPAKLQGLSPWLTCLEDGLWSLPEVYCKLECDAPPIILNANLLLPHCLQDNHDVGTICKYECKPGYYVAESAEGKVRNKLLKIQCLEGGIWEQGSCIPVVCEPPPPVFEGMYECTNGFSLDSQCVLNCNQEREKLPILCTKEGLWTQEFKLCENLQGECPPPPSELNSVEYKCEQGYGIGAVCSPLCVIPPSDPVMLPENITADTLEHWMEPVKVQSIVCTGRRQWHPDPVLVHCIQSCEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	NM_020318.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pappalysin 2 (PAPPA2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020318.2

ORF Insert Sequence:

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( NM_020318.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_064714.2 Homo sapiens pappalysin 2 (PAPPA2), transcript variant 1, mRNA.

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CloneID	OHu18995
Clone ID Related Accession (Same CDS sequence)	NM_020318.2 , XM_017002025.2 , NM_020318.3 , XM_011509857.1 , XM_017002024.1 , XM_017002025.1 , XM_011509858.2 , XM_017002023.1 , XM_005245422.3
Accession Version	NM_020318.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	pappalysin-2 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC062665.1, AF342989.1, AF311940.1 and AL031290.1. On Nov 23, 2018 this sequence version replaced NM_020318.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF342989.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000367662.5/ ENSP00000356634.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064714.2
CDS	1161..6536
Translation	MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPRASPQHHLFGVYPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRGAVEEPAAPWVGDSPIGQSELLGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTLNEPKPETQRRGWAKSRQRRQVWKRRAEDGQGDSGISSHFQPWPKHSLKHRVKKSPPEESNQNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLAEIPREAFTVEAWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKATILISHSRYQPGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFRGHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEVLQGFEPEPEILSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNPIVSEEQIRLQHEALNEAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPQVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAATWPWDKDAVTHLGGIVLSPAYYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDLCADTAPTPKSELCREPEPTSDTCGFTRFPGAPFTNYMSYTDDNCTDNFTPNQVARMHCYLDLVYQQWTESRKPTPIPIPPMVIGQTNKSLTIHWLPPISGVVYDRASGSLCGACTEDGTFRQYVHTASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPEVHLYHMNMTVPCPTEGCSLELLFQHPVQADTLTLWVTSFFMESSQVLFDTEILLENKESVHLGPLDTFCDIPLTIKLHVDGKVSGVKVYTFDERIEIDAALLTSQPHSPLCSGCRPVRYQVLRDPPFASGLPVVVTHSHRKFTDVEVTPGQMYQYQVLAEAGGELGEASPPLNHIHGAPYCGDGKVSERLGEECDDGDLVSGDGCSKVCELEEGFNCVGEPSLCYMYEGDGICEPFERKTSIVDCGIYTPKGYLDQWATRAYSSHEDKKKCPVSLVTGEPHSLICTSYHPDLPNHRPLTGWFPCVASENETQDDRSEQPEGSLKKEDEVWLKVCFNRPGEARAIFIFLTTDGLVPGEHQQPTVTLYLTDVRGSNHSLGTYGLSCQHNPLIINVTHHQNVLFHHTTSVLLNFSSPRVGISAVALRTSSRIGLSAPSNCISEDEGQNHQGQSCIHRPCGKQDSCPSLLLDHADVVNCTSIGPGLMKCAITCQRGFALQASSGQYIRPMQKEILLTCSSGHWDQNVSCLPVDCGVPDPSLVNYANFSCSEGTKFLKRCSISCVPPAKLQGLSPWLTCLEDGLWSLPEVYCKLECDAPPIILNANLLLPHCLQDNHDVGTICKYECKPGYYVAESAEGKVRNKLLKIQCLEGGIWEQGSCIPVVCEPPPPVFEGMYECTNGFSLDSQCVLNCNQEREKLPILCTKEGLWTQEFKLCENLQGECPPPPSELNSVEYKCEQGYGIGAVCSPLCVIPPSDPVMLPENITADTLEHWMEPVKVQSIVCTGRRQWHPDPVLVHCIQSCEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	NM_020318.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pappalysin 2 (PAPPA2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020318.3

ORF Insert Sequence:

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_017002025.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857514.1 Homo sapiens pappalysin 2 (PAPPA2), transcript variant X5, mRNA.

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CloneID	OHu18995
Clone ID Related Accession (Same CDS sequence)	NM_020318.2 , XM_017002025.2 , NM_020318.3 , XM_011509857.1 , XM_017002024.1 , XM_017002025.1 , XM_011509858.2 , XM_017002023.1 , XM_005245422.3
Accession Version	XM_017002025.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pappalysin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017002025.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857514.1
CDS	1417..6792
Translation	MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPRASPQHHLFGVYPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRGAVEEPAAPWVGDSPIGQSELLGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTLNEPKPETQRRGWAKSRQRRQVWKRRAEDGQGDSGISSHFQPWPKHSLKHRVKKSPPEESNQNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLAEIPREAFTVEAWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKATILISHSRYQPGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFRGHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEVLQGFEPEPEILSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNPIVSEEQIRLQHEALNEAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPQVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAATWPWDKDAVTHLGGIVLSPAYYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDLCADTAPTPKSELCREPEPTSDTCGFTRFPGAPFTNYMSYTDDNCTDNFTPNQVARMHCYLDLVYQQWTESRKPTPIPIPPMVIGQTNKSLTIHWLPPISGVVYDRASGSLCGACTEDGTFRQYVHTASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPEVHLYHMNMTVPCPTEGCSLELLFQHPVQADTLTLWVTSFFMESSQVLFDTEILLENKESVHLGPLDTFCDIPLTIKLHVDGKVSGVKVYTFDERIEIDAALLTSQPHSPLCSGCRPVRYQVLRDPPFASGLPVVVTHSHRKFTDVEVTPGQMYQYQVLAEAGGELGEASPPLNHIHGAPYCGDGKVSERLGEECDDGDLVSGDGCSKVCELEEGFNCVGEPSLCYMYEGDGICEPFERKTSIVDCGIYTPKGYLDQWATRAYSSHEDKKKCPVSLVTGEPHSLICTSYHPDLPNHRPLTGWFPCVASENETQDDRSEQPEGSLKKEDEVWLKVCFNRPGEARAIFIFLTTDGLVPGEHQQPTVTLYLTDVRGSNHSLGTYGLSCQHNPLIINVTHHQNVLFHHTTSVLLNFSSPRVGISAVALRTSSRIGLSAPSNCISEDEGQNHQGQSCIHRPCGKQDSCPSLLLDHADVVNCTSIGPGLMKCAITCQRGFALQASSGQYIRPMQKEILLTCSSGHWDQNVSCLPVDCGVPDPSLVNYANFSCSEGTKFLKRCSISCVPPAKLQGLSPWLTCLEDGLWSLPEVYCKLECDAPPIILNANLLLPHCLQDNHDVGTICKYECKPGYYVAESAEGKVRNKLLKIQCLEGGIWEQGSCIPVVCEPPPPVFEGMYECTNGFSLDSQCVLNCNQEREKLPILCTKEGLWTQEFKLCENLQGECPPPPSELNSVEYKCEQGYGIGAVCSPLCVIPPSDPVMLPENITADTLEHWMEPVKVQSIVCTGRRQWHPDPVLVHCIQSCEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_017002025.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002025.2

ORF Insert Sequence:

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_011509857.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508159.1 Homo sapiens pappalysin 2 (PAPPA2), transcript variant X1, mRNA.

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CloneID	OHu18995
Clone ID Related Accession (Same CDS sequence)	NM_020318.2 , XM_017002025.2 , NM_020318.3 , XM_011509857.1 , XM_017002024.1 , XM_017002025.1 , XM_011509858.2 , XM_017002023.1 , XM_005245422.3
Accession Version	XM_011509857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pappalysin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508159.1
CDS	1082..6457
Translation	MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPRASPQHHLFGVYPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRGAVEEPAAPWVGDSPIGQSELLGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTLNEPKPETQRRGWAKSRQRRQVWKRRAEDGQGDSGISSHFQPWPKHSLKHRVKKSPPEESNQNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLAEIPREAFTVEAWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKATILISHSRYQPGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFRGHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEVLQGFEPEPEILSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNPIVSEEQIRLQHEALNEAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPQVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAATWPWDKDAVTHLGGIVLSPAYYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDLCADTAPTPKSELCREPEPTSDTCGFTRFPGAPFTNYMSYTDDNCTDNFTPNQVARMHCYLDLVYQQWTESRKPTPIPIPPMVIGQTNKSLTIHWLPPISGVVYDRASGSLCGACTEDGTFRQYVHTASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPEVHLYHMNMTVPCPTEGCSLELLFQHPVQADTLTLWVTSFFMESSQVLFDTEILLENKESVHLGPLDTFCDIPLTIKLHVDGKVSGVKVYTFDERIEIDAALLTSQPHSPLCSGCRPVRYQVLRDPPFASGLPVVVTHSHRKFTDVEVTPGQMYQYQVLAEAGGELGEASPPLNHIHGAPYCGDGKVSERLGEECDDGDLVSGDGCSKVCELEEGFNCVGEPSLCYMYEGDGICEPFERKTSIVDCGIYTPKGYLDQWATRAYSSHEDKKKCPVSLVTGEPHSLICTSYHPDLPNHRPLTGWFPCVASENETQDDRSEQPEGSLKKEDEVWLKVCFNRPGEARAIFIFLTTDGLVPGEHQQPTVTLYLTDVRGSNHSLGTYGLSCQHNPLIINVTHHQNVLFHHTTSVLLNFSSPRVGISAVALRTSSRIGLSAPSNCISEDEGQNHQGQSCIHRPCGKQDSCPSLLLDHADVVNCTSIGPGLMKCAITCQRGFALQASSGQYIRPMQKEILLTCSSGHWDQNVSCLPVDCGVPDPSLVNYANFSCSEGTKFLKRCSISCVPPAKLQGLSPWLTCLEDGLWSLPEVYCKLECDAPPIILNANLLLPHCLQDNHDVGTICKYECKPGYYVAESAEGKVRNKLLKIQCLEGGIWEQGSCIPVVCEPPPPVFEGMYECTNGFSLDSQCVLNCNQEREKLPILCTKEGLWTQEFKLCENLQGECPPPPSELNSVEYKCEQGYGIGAVCSPLCVIPPSDPVMLPENITADTLEHWMEPVKVQSIVCTGRRQWHPDPVLVHCIQSCEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_011509857.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509857.1

ORF Insert Sequence:

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_017002024.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857513.1 Homo sapiens pappalysin 2 (PAPPA2), transcript variant X3, mRNA.

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CloneID	OHu18995
Clone ID Related Accession (Same CDS sequence)	NM_020318.2 , XM_017002025.2 , NM_020318.3 , XM_011509857.1 , XM_017002024.1 , XM_017002025.1 , XM_011509858.2 , XM_017002023.1 , XM_005245422.3
Accession Version	XM_017002024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pappalysin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857513.1
CDS	1310..6685
Translation	MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPRASPQHHLFGVYPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRGAVEEPAAPWVGDSPIGQSELLGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTLNEPKPETQRRGWAKSRQRRQVWKRRAEDGQGDSGISSHFQPWPKHSLKHRVKKSPPEESNQNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLAEIPREAFTVEAWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKATILISHSRYQPGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFRGHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEVLQGFEPEPEILSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNPIVSEEQIRLQHEALNEAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPQVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAATWPWDKDAVTHLGGIVLSPAYYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDLCADTAPTPKSELCREPEPTSDTCGFTRFPGAPFTNYMSYTDDNCTDNFTPNQVARMHCYLDLVYQQWTESRKPTPIPIPPMVIGQTNKSLTIHWLPPISGVVYDRASGSLCGACTEDGTFRQYVHTASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPEVHLYHMNMTVPCPTEGCSLELLFQHPVQADTLTLWVTSFFMESSQVLFDTEILLENKESVHLGPLDTFCDIPLTIKLHVDGKVSGVKVYTFDERIEIDAALLTSQPHSPLCSGCRPVRYQVLRDPPFASGLPVVVTHSHRKFTDVEVTPGQMYQYQVLAEAGGELGEASPPLNHIHGAPYCGDGKVSERLGEECDDGDLVSGDGCSKVCELEEGFNCVGEPSLCYMYEGDGICEPFERKTSIVDCGIYTPKGYLDQWATRAYSSHEDKKKCPVSLVTGEPHSLICTSYHPDLPNHRPLTGWFPCVASENETQDDRSEQPEGSLKKEDEVWLKVCFNRPGEARAIFIFLTTDGLVPGEHQQPTVTLYLTDVRGSNHSLGTYGLSCQHNPLIINVTHHQNVLFHHTTSVLLNFSSPRVGISAVALRTSSRIGLSAPSNCISEDEGQNHQGQSCIHRPCGKQDSCPSLLLDHADVVNCTSIGPGLMKCAITCQRGFALQASSGQYIRPMQKEILLTCSSGHWDQNVSCLPVDCGVPDPSLVNYANFSCSEGTKFLKRCSISCVPPAKLQGLSPWLTCLEDGLWSLPEVYCKLECDAPPIILNANLLLPHCLQDNHDVGTICKYECKPGYYVAESAEGKVRNKLLKIQCLEGGIWEQGSCIPVVCEPPPPVFEGMYECTNGFSLDSQCVLNCNQEREKLPILCTKEGLWTQEFKLCENLQGECPPPPSELNSVEYKCEQGYGIGAVCSPLCVIPPSDPVMLPENITADTLEHWMEPVKVQSIVCTGRRQWHPDPVLVHCIQSCEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_017002024.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002024.1

ORF Insert Sequence:

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_017002025.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857514.1 Homo sapiens pappalysin 2 (PAPPA2), transcript variant X5, mRNA.

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CloneID	OHu18995
Clone ID Related Accession (Same CDS sequence)	NM_020318.2 , XM_017002025.2 , NM_020318.3 , XM_011509857.1 , XM_017002024.1 , XM_017002025.1 , XM_011509858.2 , XM_017002023.1 , XM_005245422.3
Accession Version	XM_017002025.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	pappalysin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857514.1
CDS	1416..6791
Translation	MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPRASPQHHLFGVYPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRGAVEEPAAPWVGDSPIGQSELLGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTLNEPKPETQRRGWAKSRQRRQVWKRRAEDGQGDSGISSHFQPWPKHSLKHRVKKSPPEESNQNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLAEIPREAFTVEAWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKATILISHSRYQPGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFRGHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEVLQGFEPEPEILSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNPIVSEEQIRLQHEALNEAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPQVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAATWPWDKDAVTHLGGIVLSPAYYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDLCADTAPTPKSELCREPEPTSDTCGFTRFPGAPFTNYMSYTDDNCTDNFTPNQVARMHCYLDLVYQQWTESRKPTPIPIPPMVIGQTNKSLTIHWLPPISGVVYDRASGSLCGACTEDGTFRQYVHTASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPEVHLYHMNMTVPCPTEGCSLELLFQHPVQADTLTLWVTSFFMESSQVLFDTEILLENKESVHLGPLDTFCDIPLTIKLHVDGKVSGVKVYTFDERIEIDAALLTSQPHSPLCSGCRPVRYQVLRDPPFASGLPVVVTHSHRKFTDVEVTPGQMYQYQVLAEAGGELGEASPPLNHIHGAPYCGDGKVSERLGEECDDGDLVSGDGCSKVCELEEGFNCVGEPSLCYMYEGDGICEPFERKTSIVDCGIYTPKGYLDQWATRAYSSHEDKKKCPVSLVTGEPHSLICTSYHPDLPNHRPLTGWFPCVASENETQDDRSEQPEGSLKKEDEVWLKVCFNRPGEARAIFIFLTTDGLVPGEHQQPTVTLYLTDVRGSNHSLGTYGLSCQHNPLIINVTHHQNVLFHHTTSVLLNFSSPRVGISAVALRTSSRIGLSAPSNCISEDEGQNHQGQSCIHRPCGKQDSCPSLLLDHADVVNCTSIGPGLMKCAITCQRGFALQASSGQYIRPMQKEILLTCSSGHWDQNVSCLPVDCGVPDPSLVNYANFSCSEGTKFLKRCSISCVPPAKLQGLSPWLTCLEDGLWSLPEVYCKLECDAPPIILNANLLLPHCLQDNHDVGTICKYECKPGYYVAESAEGKVRNKLLKIQCLEGGIWEQGSCIPVVCEPPPPVFEGMYECTNGFSLDSQCVLNCNQEREKLPILCTKEGLWTQEFKLCENLQGECPPPPSELNSVEYKCEQGYGIGAVCSPLCVIPPSDPVMLPENITADTLEHWMEPVKVQSIVCTGRRQWHPDPVLVHCIQSCEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_017002025.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002025.1

ORF Insert Sequence:

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_011509858.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508160.1 Homo sapiens pappalysin 2 (PAPPA2), transcript variant X6, mRNA.

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CloneID	OHu18995
Clone ID Related Accession (Same CDS sequence)	NM_020318.2 , XM_017002025.2 , NM_020318.3 , XM_011509857.1 , XM_017002024.1 , XM_017002025.1 , XM_011509858.2 , XM_017002023.1 , XM_005245422.3
Accession Version	XM_011509858.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pappalysin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509858.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508160.1
CDS	1425..6800
Translation	MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPRASPQHHLFGVYPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRGAVEEPAAPWVGDSPIGQSELLGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTLNEPKPETQRRGWAKSRQRRQVWKRRAEDGQGDSGISSHFQPWPKHSLKHRVKKSPPEESNQNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLAEIPREAFTVEAWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKATILISHSRYQPGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFRGHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEVLQGFEPEPEILSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNPIVSEEQIRLQHEALNEAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPQVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAATWPWDKDAVTHLGGIVLSPAYYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDLCADTAPTPKSELCREPEPTSDTCGFTRFPGAPFTNYMSYTDDNCTDNFTPNQVARMHCYLDLVYQQWTESRKPTPIPIPPMVIGQTNKSLTIHWLPPISGVVYDRASGSLCGACTEDGTFRQYVHTASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPEVHLYHMNMTVPCPTEGCSLELLFQHPVQADTLTLWVTSFFMESSQVLFDTEILLENKESVHLGPLDTFCDIPLTIKLHVDGKVSGVKVYTFDERIEIDAALLTSQPHSPLCSGCRPVRYQVLRDPPFASGLPVVVTHSHRKFTDVEVTPGQMYQYQVLAEAGGELGEASPPLNHIHGAPYCGDGKVSERLGEECDDGDLVSGDGCSKVCELEEGFNCVGEPSLCYMYEGDGICEPFERKTSIVDCGIYTPKGYLDQWATRAYSSHEDKKKCPVSLVTGEPHSLICTSYHPDLPNHRPLTGWFPCVASENETQDDRSEQPEGSLKKEDEVWLKVCFNRPGEARAIFIFLTTDGLVPGEHQQPTVTLYLTDVRGSNHSLGTYGLSCQHNPLIINVTHHQNVLFHHTTSVLLNFSSPRVGISAVALRTSSRIGLSAPSNCISEDEGQNHQGQSCIHRPCGKQDSCPSLLLDHADVVNCTSIGPGLMKCAITCQRGFALQASSGQYIRPMQKEILLTCSSGHWDQNVSCLPVDCGVPDPSLVNYANFSCSEGTKFLKRCSISCVPPAKLQGLSPWLTCLEDGLWSLPEVYCKLECDAPPIILNANLLLPHCLQDNHDVGTICKYECKPGYYVAESAEGKVRNKLLKIQCLEGGIWEQGSCIPVVCEPPPPVFEGMYECTNGFSLDSQCVLNCNQEREKLPILCTKEGLWTQEFKLCENLQGECPPPPSELNSVEYKCEQGYGIGAVCSPLCVIPPSDPVMLPENITADTLEHWMEPVKVQSIVCTGRRQWHPDPVLVHCIQSCEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_011509858.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509858.2

ORF Insert Sequence:

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_017002023.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857512.1 Homo sapiens pappalysin 2 (PAPPA2), transcript variant X2, mRNA.

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CloneID	OHu18995
Clone ID Related Accession (Same CDS sequence)	NM_020318.2 , XM_017002025.2 , NM_020318.3 , XM_011509857.1 , XM_017002024.1 , XM_017002025.1 , XM_011509858.2 , XM_017002023.1 , XM_005245422.3
Accession Version	XM_017002023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pappalysin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857512.1
CDS	1263..6638
Translation	MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPRASPQHHLFGVYPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRGAVEEPAAPWVGDSPIGQSELLGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTLNEPKPETQRRGWAKSRQRRQVWKRRAEDGQGDSGISSHFQPWPKHSLKHRVKKSPPEESNQNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLAEIPREAFTVEAWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKATILISHSRYQPGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFRGHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEVLQGFEPEPEILSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNPIVSEEQIRLQHEALNEAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPQVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAATWPWDKDAVTHLGGIVLSPAYYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDLCADTAPTPKSELCREPEPTSDTCGFTRFPGAPFTNYMSYTDDNCTDNFTPNQVARMHCYLDLVYQQWTESRKPTPIPIPPMVIGQTNKSLTIHWLPPISGVVYDRASGSLCGACTEDGTFRQYVHTASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPEVHLYHMNMTVPCPTEGCSLELLFQHPVQADTLTLWVTSFFMESSQVLFDTEILLENKESVHLGPLDTFCDIPLTIKLHVDGKVSGVKVYTFDERIEIDAALLTSQPHSPLCSGCRPVRYQVLRDPPFASGLPVVVTHSHRKFTDVEVTPGQMYQYQVLAEAGGELGEASPPLNHIHGAPYCGDGKVSERLGEECDDGDLVSGDGCSKVCELEEGFNCVGEPSLCYMYEGDGICEPFERKTSIVDCGIYTPKGYLDQWATRAYSSHEDKKKCPVSLVTGEPHSLICTSYHPDLPNHRPLTGWFPCVASENETQDDRSEQPEGSLKKEDEVWLKVCFNRPGEARAIFIFLTTDGLVPGEHQQPTVTLYLTDVRGSNHSLGTYGLSCQHNPLIINVTHHQNVLFHHTTSVLLNFSSPRVGISAVALRTSSRIGLSAPSNCISEDEGQNHQGQSCIHRPCGKQDSCPSLLLDHADVVNCTSIGPGLMKCAITCQRGFALQASSGQYIRPMQKEILLTCSSGHWDQNVSCLPVDCGVPDPSLVNYANFSCSEGTKFLKRCSISCVPPAKLQGLSPWLTCLEDGLWSLPEVYCKLECDAPPIILNANLLLPHCLQDNHDVGTICKYECKPGYYVAESAEGKVRNKLLKIQCLEGGIWEQGSCIPVVCEPPPPVFEGMYECTNGFSLDSQCVLNCNQEREKLPILCTKEGLWTQEFKLCENLQGECPPPPSELNSVEYKCEQGYGIGAVCSPLCVIPPSDPVMLPENITADTLEHWMEPVKVQSIVCTGRRQWHPDPVLVHCIQSCEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_017002023.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002023.1

ORF Insert Sequence:

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_005245422.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245479.1 Homo sapiens pappalysin 2 (PAPPA2), transcript variant X4, mRNA.

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CloneID	OHu18995
Clone ID Related Accession (Same CDS sequence)	NM_020318.2 , XM_017002025.2 , NM_020318.3 , XM_011509857.1 , XM_017002024.1 , XM_017002025.1 , XM_011509858.2 , XM_017002023.1 , XM_005245422.3
Accession Version	XM_005245422.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pappalysin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245422.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245479.1
CDS	1212..6587
Translation	MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPRASPQHHLFGVYPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRGAVEEPAAPWVGDSPIGQSELLGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTLNEPKPETQRRGWAKSRQRRQVWKRRAEDGQGDSGISSHFQPWPKHSLKHRVKKSPPEESNQNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLAEIPREAFTVEAWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKATILISHSRYQPGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFRGHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEVLQGFEPEPEILSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNPIVSEEQIRLQHEALNEAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPQVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAATWPWDKDAVTHLGGIVLSPAYYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDLCADTAPTPKSELCREPEPTSDTCGFTRFPGAPFTNYMSYTDDNCTDNFTPNQVARMHCYLDLVYQQWTESRKPTPIPIPPMVIGQTNKSLTIHWLPPISGVVYDRASGSLCGACTEDGTFRQYVHTASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPEVHLYHMNMTVPCPTEGCSLELLFQHPVQADTLTLWVTSFFMESSQVLFDTEILLENKESVHLGPLDTFCDIPLTIKLHVDGKVSGVKVYTFDERIEIDAALLTSQPHSPLCSGCRPVRYQVLRDPPFASGLPVVVTHSHRKFTDVEVTPGQMYQYQVLAEAGGELGEASPPLNHIHGAPYCGDGKVSERLGEECDDGDLVSGDGCSKVCELEEGFNCVGEPSLCYMYEGDGICEPFERKTSIVDCGIYTPKGYLDQWATRAYSSHEDKKKCPVSLVTGEPHSLICTSYHPDLPNHRPLTGWFPCVASENETQDDRSEQPEGSLKKEDEVWLKVCFNRPGEARAIFIFLTTDGLVPGEHQQPTVTLYLTDVRGSNHSLGTYGLSCQHNPLIINVTHHQNVLFHHTTSVLLNFSSPRVGISAVALRTSSRIGLSAPSNCISEDEGQNHQGQSCIHRPCGKQDSCPSLLLDHADVVNCTSIGPGLMKCAITCQRGFALQASSGQYIRPMQKEILLTCSSGHWDQNVSCLPVDCGVPDPSLVNYANFSCSEGTKFLKRCSISCVPPAKLQGLSPWLTCLEDGLWSLPEVYCKLECDAPPIILNANLLLPHCLQDNHDVGTICKYECKPGYYVAESAEGKVRNKLLKIQCLEGGIWEQGSCIPVVCEPPPPVFEGMYECTNGFSLDSQCVLNCNQEREKLPILCTKEGLWTQEFKLCENLQGECPPPPSELNSVEYKCEQGYGIGAVCSPLCVIPPSDPVMLPENITADTLEHWMEPVKVQSIVCTGRRQWHPDPVLVHCIQSCEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_005245422.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pappalysin 2 (PAPPA2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245422.3

ORF Insert Sequence:

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GATGATAACT GCACTGACAA CTTCACTCCT 
AACCAAGTGG CCCGAATGCA TTGCTATTTG GACCTAGTCT ATCAGCAGTG GACTGAAAGC 
AGAAAGCCCA CCCCCATCCC CATTCCACCT ATGGTCATCG GACAGACCAA CAAGTCCCTC 
ACTATCCACT GGCTGCCTCC TATTAGTGGA GTTGTATATG ACAGGGCCTC AGGCAGCTTG 
TGTGGCGCTT GCACTGAAGA TGGGACCTTT CGTCAGTATG TGCACACAGC TTCCTCCCGG 
CGGGTGTGTG ACTCCTCAGG TTATTGGACC CCAGAGGAGG CTGTGGGGCC TCCTGATGTG 
GATCAGCCCT GCGAGCCAAG CTTACAGGCC TGGAGCCCTG AGGTCCACCT GTACCACATG 
AACATGACGG TCCCCTGCCC CACAGAAGGC TGTAGCTTGG AGCTGCTCTT CCAACACCCG 
GTCCAAGCCG ACACCCTCAC CCTGTGGGTC ACTTCCTTCT TCATGGAGTC CTCGCAGGTC 
CTCTTTGACA CAGAGATCTT GCTGGAAAAC AAGGAGTCAG TGCACCTGGG CCCCTTAGAC 
ACTTTCTGTG ACATCCCACT CACCATCAAA CTGCACGTGG ATGGGAAGGT GTCGGGGGTG 
AAAGTCTACA CCTTTGATGA GAGGATAGAG ATTGATGCAG CACTCCTGAC TTCTCAGCCC 
CACAGTCCCT TGTGCTCTGG CTGCAGGCCT GTGAGGTACC AGGTTCTCCG CGATCCCCCA 
TTTGCCAGTG GTTTGCCCGT GGTGGTGACA CATTCTCACA GGAAGTTCAC GGACGTGGAG 
GTCACACCTG GACAGATGTA TCAGTACCAA GTTCTAGCTG AAGCTGGAGG AGAACTGGGA 
GAAGCTTCGC CTCCTCTGAA CCACATTCAT GGAGCTCCTT ATTGTGGAGA TGGGAAGGTG 
TCAGAGAGAC TGGGAGAAGA GTGTGATGAT GGAGACCTTG TGAGCGGAGA TGGCTGCTCC 
AAGGTGTGTG AGCTGGAGGA AGGTTTCAAC TGTGTAGGAG AGCCAAGCCT TTGCTACATG 
TATGAGGGAG ATGGCATATG TGAACCTTTT GAGAGAAAAA CCAGCATTGT AGACTGTGGC 
ATCTACACTC CCAAAGGATA CTTGGATCAA TGGGCTACCC GGGCTTACTC CTCTCATGAA 
GACAAGAAGA AGTGTCCTGT TTCCTTGGTA ACTGGAGAAC CTCATTCCCT AATTTGCACA 
TCATACCATC CAGATTTACC CAACCACCGT CCCCTAACTG GCTGGTTTCC CTGTGTTGCC 
AGTGAAAATG AAACTCAGGA TGACAGGAGT GAACAGCCAG AAGGTAGCCT GAAGAAAGAG 
GATGAGGTTT GGCTCAAAGT GTGTTTCAAT AGACCAGGAG AGGCCAGAGC AATTTTTATT 
TTTTTGACAA CTGATGGCCT AGTTCCCGGA GAGCATCAGC AGCCGACAGT GACTCTCTAC 
CTGACCGATG TCCGTGGAAG CAACCACTCT CTTGGAACCT ATGGACTGTC ATGCCAGCAT 
AATCCACTGA TTATCAATGT GACCCATCAC CAGAATGTCC TTTTCCACCA TACCACCTCA 
GTGCTGCTGA ATTTCTCATC CCCACGGGTC GGCATCTCAG CTGTGGCTCT AAGGACATCC 
TCCCGCATTG GTCTTTCGGC TCCCAGTAAC TGCATCTCAG AGGACGAGGG GCAGAATCAT 
CAGGGACAGA GCTGTATCCA TCGGCCCTGT GGGAAGCAGG ACAGCTGTCC GTCATTGCTG 
CTTGATCATG CTGATGTGGT GAACTGTACC TCTATAGGCC CAGGTCTCAT GAAGTGTGCT 
ATCACTTGTC AAAGGGGATT TGCCCTTCAG GCCAGCAGTG GGCAGTACAT CAGGCCCATG 
CAGAAGGAAA TTCTGCTCAC ATGTTCTTCT GGGCACTGGG ACCAGAATGT GAGCTGCCTT 
CCCGTGGACT GCGGTGTTCC CGACCCGTCT TTGGTGAACT ATGCAAACTT CTCCTGCTCA 
GAGGGAACCA AATTTCTGAA ACGCTGCTCA ATCTCTTGTG TCCCACCAGC CAAGCTGCAA 
GGACTGAGCC CATGGCTGAC ATGTCTTGAA GATGGTCTCT GGTCTCTCCC TGAAGTCTAC 
TGCAAGTTGG AGTGTGATGC TCCCCCTATT ATTCTGAATG CCAACTTGCT CCTGCCTCAC 
TGCCTCCAGG ACAACCACGA CGTGGGCACC ATCTGCAAAT ATGAATGCAA ACCAGGGTAC 
TATGTGGCAG AAAGTGCAGA GGGTAAAGTC AGGAACAAGC TCCTGAAGAT ACAATGCCTG 
GAAGGTGGAA TCTGGGAGCA AGGCAGCTGC ATTCCTGTGG TGTGTGAGCC ACCCCCTCCT 
GTGTTTGAAG GCATGTATGA ATGTACCAAT GGCTTCAGCC TGGACAGCCA GTGTGTGCTC 
AACTGTAACC AGGAACGTGA AAAGCTTCCC ATCCTCTGCA CTAAAGAGGG CCTGTGGACC 
CAGGAGTTTA AGTTGTGTGA GAATCTGCAA GGAGAATGCC CACCACCCCC CTCAGAGCTG 
AATTCTGTGG AGTACAAATG TGAACAAGGA TATGGGATTG GTGCAGTGTG TTCCCCATTG 
TGTGTAATCC CCCCCAGTGA CCCCGTGATG CTACCTGAGA ATATCACTGC TGACACTCTG 
GAGCACTGGA TGGAACCTGT CAAAGTCCAG AGCATTGTGT GCACTGGCCG GCGTCAATGG 
CACCCAGACC CCGTCTTAGT CCACTGCATC CAGTCATGTG AGCCCTTCCA AGCAGATGGT 
TGGTGTGACA CTATCAACAA CCGAGCCTAC TGCCACTATG ACGGGGGAGA CTGCTGCTCT 
TCCACACTCT CCTCCAAGAA GGTCATTCCA TTTGCTGCTG ACTGTGACCT GGATGAGTGC 
ACCTGCCGGG ACCCCAAGGC AGAAGAAAAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( NM_021936.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_068755.2 Homo sapiens pappalysin 2 (PAPPA2), transcript variant 2, mRNA.

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CloneID	OHu29954
Clone ID Related Accession (Same CDS sequence)	NM_021936.2 , NM_021936.3
Accession Version	NM_021936.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	pappalysin-2 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC062665.1, BC117195.1, AL596254.2 and AL139282.10. On Oct 14, 2006 this sequence version replaced NM_021936.1. Transcript Variant: # This Variant: This variant (2) differs in the 3' UTR and has multiple coding region differences, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF342990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END##

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GGTATCACCA CTGTCTTGTT TTGTTTTCTG 
TTAAGAATAC ATGGGGGCCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068755.2
CDS	1165..3648
Translation	MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPRASPQHHLFGVYPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRGAVEEPAAPWVGDSPIGQSELLGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTLNEPKPETQRRGWAKSRQRRQVWKRRAEDGQGDSGISSHFQPWPKHSLKHRVKKSPPEESNQNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLAEIPREAFTVEAWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKATILISHSRYQPGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFRGHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEVLQGFEPEPEILSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNPIVSEEQIRLQHEALNEAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPQVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAATWPWDKDAVTHLGGIVLSPAYYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDLCADTAPTPKSELCREPEPTSDTCGFTRFPGAPFTNYMSYTGITTVLFCFLLRIHGGL

Target ORF information:

RefSeq Version	NM_021936.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pappalysin 2 (PAPPA2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021936.2

ORF Insert Sequence:

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GGTATCACCA CTGTCTTGTT TTGTTTTCTG 
TTAAGAATAC ATGGGGGCCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( NM_021936.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_068755.2 Homo sapiens pappalysin 2 (PAPPA2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu29954
Clone ID Related Accession (Same CDS sequence)	NM_021936.2 , NM_021936.3
Accession Version	NM_021936.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	pappalysin-2 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC062665.1, BC117195.1, AL596254.2 and AL139282.10. On May 31, 2019 this sequence version replaced NM_021936.2. Transcript Variant: # This Variant: This variant (2) differs in the 3' UTR and has multiple coding region differences, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF342990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END##

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GGTATCACCA CTGTCTTGTT TTGTTTTCTG 
TTAAGAATAC ATGGGGGCCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068755.2
CDS	1161..3644
Translation	MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPRASPQHHLFGVYPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRGAVEEPAAPWVGDSPIGQSELLGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTLNEPKPETQRRGWAKSRQRRQVWKRRAEDGQGDSGISSHFQPWPKHSLKHRVKKSPPEESNQNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLAEIPREAFTVEAWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKATILISHSRYQPGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFRGHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEVLQGFEPEPEILSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNPIVSEEQIRLQHEALNEAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPQVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAATWPWDKDAVTHLGGIVLSPAYYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDLCADTAPTPKSELCREPEPTSDTCGFTRFPGAPFTNYMSYTGITTVLFCFLLRIHGGL

Target ORF information:

RefSeq Version	NM_021936.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pappalysin 2 (PAPPA2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021936.3

ORF Insert Sequence:

ATGATGTGCT TAAAGATCCT AAGAATAAGC CTGGCGATTT TGGCTGGGTG GGCACTCTGT 
TCTGCCAACT CTGAGCTGGG CTGGACACGC AAGAAATCCT TGGTTGAGAG GGAACACCTG 
AATCAGGTGC TGTTGGAAGG AGAACGTTGT TGGCTGGGGG CCAAGGTTCG AAGACCCAGA 
GCTTCTCCAC AGCATCACCT CTTTGGAGTC TACCCCAGCA GGGCTGGGAA CTACCTAAGG 
CCCTACCCCG TGGGGGAGCA AGAAATCCAT CATACAGGAC GCAGCAAACC AGACACTGAA 
GGAAATGCTG TGAGCCTTGT TCCCCCAGAC CTGACTGAAA ATCCAGCAGG ACTGAGGGGT 
GCAGTTGAAG AGCCGGCTGC CCCATGGGTA GGGGATAGTC CTATTGGGCA ATCTGAGCTG 
CTGGGAGATG ATGACGCTTA TCTCGGCAAT CAAAGATCCA AGGAGTCTCT AGGTGAGGCC 
GGGATTCAGA AAGGCTCAGC CATGGCTGCC ACTACTACCA CCGCCATTTT CACAACCCTG 
AACGAACCCA AACCAGAGAC CCAAAGGAGG GGCTGGGCCA AGTCCAGGCA GCGTCGCCAA 
GTGTGGAAGA GGCGGGCGGA AGATGGGCAG GGAGACTCCG GTATCTCTTC ACATTTCCAA 
CCTTGGCCCA AGCATTCCCT TAAACACAGG GTCAAAAAGA GTCCACCGGA GGAAAGCAAC 
CAAAATGGTG GAGAGGGCTC CTACCGAGAA GCAGAGACCT TTAACTCCCA AGTAGGACTG 
CCCATCTTAT ACTTCTCTGG GAGGCGGGAG CGGCTGCTGC TGCGTCCAGA AGTGCTGGCT 
GAGATTCCCC GGGAGGCGTT CACAGTGGAA GCCTGGGTTA AACCGGAGGG AGGACAGAAC 
AACCCAGCCA TCATCGCAGG TGTGTTTGAT AACTGCTCCC ACACTGTCAG TGACAAAGGC 
TGGGCCCTGG GGATCCGCTC AGGGAAGGAC AAGGGAAAGC GGGATGCTCG CTTCTTCTTC 
TCCCTCTGCA CCGACCGCGT GAAGAAAGCC ACCATCTTGA TTAGCCACAG TCGCTACCAA 
CCAGGCACAT GGACCCATGT GGCAGCCACT TACGATGGAC GGCACATGGC CCTGTATGTG 
GATGGCACTC AGGTGGCTAG CAGTCTAGAC CAGTCTGGTC CCCTGAACAG CCCCTTCATG 
GCATCTTGCC GCTCTTTGCT CCTGGGGGGA GACAGCTCTG AGGATGGGCA CTATTTCCGT 
GGACACCTGG GCACACTGGT TTTCTGGTCG ACCGCCCTGC CACAAAGCCA TTTTCAGCAC 
AGTTCTCAGC ATTCAAGTGG GGAGGAGGAA GCGACTGACT TGGTCCTGAC AGCGAGCTTT 
GAGCCTGTGA ACACAGAGTG GGTTCCCTTT AGAGATGAGA AGTACCCACG ACTTGAGGTT 
CTCCAGGGCT TTGAGCCAGA GCCTGAGATT CTGTCGCCTT TGCAGCCCCC ACTCTGTGGG 
CAAACAGTCT GTGACAATGT GGAATTGATC TCCCAGTACA ATGGATACTG GCCCCTTCGG 
GGAGAGAAGG TGATACGCTA CCAGGTGGTG AACATCTGTG ATGATGAGGG CCTAAACCCC 
ATTGTGAGTG AGGAGCAGAT TCGTCTGCAG CACGAGGCAC TGAATGAGGC CTTCAGCCGC 
TACAACATCA GCTGGCAGCT GAGCGTCCAC CAGGTCCACA ATTCCACCCT GCGACACCGG 
GTTGTGCTTG TGAACTGTGA GCCCAGCAAG ATTGGCAATG ACCATTGTGA CCCCGAGTGT 
GAGCACCCAC TCACAGGCTA TGATGGGGGT GACTGCCGCC TGCAGGGCCG CTGCTACTCC 
TGGAACCGCA GGGATGGGCT CTGTCACGTG GAGTGTAACA ACATGCTGAA CGACTTTGAC 
GACGGAGACT GCTGCGACCC CCAGGTGGCT GATGTGCGCA AGACCTGCTT TGACCCTGAC 
TCACCCAAGA GGGCATACAT GAGTGTGAAG GAGCTGAAGG AGGCCCTGCA GCTGAACAGT 
ACTCACTTCC TCAACATCTA CTTTGCCAGC TCAGTGCGGG AAGACCTTGC AGGTGCTGCC 
ACCTGGCCTT GGGACAAGGA CGCTGTCACT CACCTGGGTG GCATTGTCCT CAGCCCAGCA 
TATTATGGGA TGCCTGGCCA CACCGACACC ATGATCCATG AAGTGGGACA TGTTCTGGGA 
CTCTACCATG TCTTTAAAGG AGTCAGTGAA AGAGAATCCT GCAATGACCC CTGCAAGGAG 
ACAGTGCCAT CCATGGAAAC GGGAGACCTC TGTGCCGACA CCGCCCCCAC TCCCAAGAGT 
GAGCTGTGCC GGGAACCAGA GCCCACTAGT GACACCTGTG GCTTCACTCG CTTCCCAGGG 
GCTCCGTTCA CCAACTACAT GAGCTACACG GGTATCACCA CTGTCTTGTT TTGTTTTCTG 
TTAAGAATAC ATGGGGGCCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PAPPA2
Entrez Gene ID	60676
Full Name	pappalysin 2
Synonyms	PAPP-A2,PAPP-E,PAPPE,PLAC3
General protein information	Preferred Names pappalysin 2 Names pappalysin-2 placenta-specific 3 pregnancy-associated plasma preproprotein-A2 pregnancy-associated plasma protein E1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q25.2
Summary	This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010].

Danio rerio (zebrafish)	pappa2	XP_695417.4
Homo sapiens (human)	PAPPA2	NP_064714.2
Mus musculus (house mouse)	Pappa2	NP_001078845.1
	PAPPA2	XP_002808308.1
Rattus norvegicus (Norway rat)	LOC680415	XP_002728071.2
Pan troglodytes (chimpanzee)	PAPPA2	XP_514020.2
Canis lupus familiaris (dog)	PAPPA2	XP_537179.2
Bos taurus (cattle)	PAPPA2	XP_002694146.1
Gallus gallus (chicken)	PAPPA2	XP_426627.4
Xenopus tropicalis (tropical clawed frog)	pappa2	XP_002931515.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PAPPA2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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