MAGEE1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAGEE1
Entrez Gene ID	57692
Full Name	MAGE family member E1
Synonyms	DAMAGE,HCA1
General protein information	Preferred Names MAGE family member E1 Names melanoma-associated antigen E1 MAGE-E1 antigen alpha-dystrobrevin-associated MAGE Protein dystrobrevin-associated MAGE protein hepatocellular carcinoma-associated HCA1 hepatocellular carcinoma-associated protein 1 melanoma antigen family E, 1 melanoma antigen family E1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq13.3
Summary	This gene encodes an alpha-dystrobrevin-associated MAGE (melanoma-associated antigen) protein, which is a member of the MAGE family. The protein contains a nuclear localization signal in the N-terminus, 30 12-amino acid repeats beginning at nt 60 with the consensus sequence ASEGPSTSVLPT, and two MAGE domains in the C-terminus. It may play a signaling role in brain, muscle, and peripheral nerve. This gene is located on X chromosome in a region containing loci linked to cognitive disability. [provided by RefSeq, Mar 2010].
Disorder MIM:	300759

mRNA and Protein(s)

mRNA	Protein	Name
NM_020932.2	NP_065983.1	melanoma-associated antigen E1
NM_020932.3	NP_065983.1	melanoma-associated antigen E1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Magee1	NP_444431.3
Homo sapiens (human)	MAGEE1	NP_065983.1
Pan troglodytes (chimpanzee)	MAGEE1	XP_001143922.2
Rattus norvegicus (Norway rat)	Magee1	NP_001073360.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Naturally occurring HCA1 missense mutations result in loss of function: potential impact on lipid deposition.
Doyle JR, Lane JM, Beinborn M, Kopin AS
Journal of lipid research54(3)823-30(2013 Mar)

MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.
Doyle JM, Gao J, Wang J, Yang M, Potts PR
Molecular cell39(6)963-74(2010 Sep)

Frequent MAGE mutations in human melanoma.
Caballero OL, Zhao Q, Rimoldi D, Stevenson BJ, Svobodov? S, Devalle S, R?hrig UF, Pagotto A, Michielin O, Speiser D, Wolchok JD, Liu C, Pejovic T, Odunsi K, Brasseur F, Van den Eynde BJ, Old LJ, Lu X, Cebon J, Strausberg RL, Simpson AJ
PloS one5(9)(2010 Sep)

DAMAGE, a novel alpha-dystrobrevin-associated MAGE protein in dystrophin complexes.
Albrecht DE, Froehner SC
The Journal of biological chemistry279(8)7014-23(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that lipid homeostasis may be altered in carriers of selected HCA1 missense variants.
Title: Naturally occurring HCA1 missense mutations result in loss of function: potential impact on lipid deposition.

Observational study of gene-disease association. (HuGE Navigator)
Title: Frequent MAGE mutations in human melanoma.

a new dystrobrevin-associated protein that may play a signaling role in brain, muscle, and peripheral nerve
Title: DAMAGE, a novel alpha-dystrobrevin-associated MAGE protein in dystrophin complexes.

The following MAGEE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGEE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19574	NM_020932.3 Latest version!	Homo sapiens MAGE family member E1 (MAGEE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu19574	NM_020932.2	Homo sapiens MAGE family member E1 (MAGEE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAGEE1 ( NM_020932.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065983.1 Homo sapiens MAGE family member E1 (MAGEE1), mRNA.

Price & Availability↑

CloneID	OHu19574
Clone ID Related Accession (Same CDS sequence)	NM_020932.2 , NM_020932.3
Accession Version	NM_020932.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	melanoma-associated antigen E1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA411859.1, AB046807.1 and BC050588.1. This sequence is a reference standard in the RefSeqGene project. On Mar 13, 2010 this sequence version replaced NM_020932.1.

ATGTCTCTGG TAAGCCAGAA TTCGCGCCGC CGCCGCCGCC GCGTTGCAAA GGCTACTGCG 
CACAACAGCA GCTGGGGCGA AATGCAGGCC CCTAATGCCC CCGGTCTCCC CGCTGATGTG 
CCAGGCTCAG ACGTCCCCCA GGGTCCCAGC GATTCCCAGA TCCTCCAGGG CCTCTGCGCC 
TCTGAGGGCC CAAGCACCTC CGTTCTGCCC ACCTCCGCTG AGGGCCCAAG CACCTTTGTG 
CCGCCCACCA TCTCTGAGGC CTCAAGCGCC TCCGGGCAGC CCACCATCTC TGAGGGACCT 
GGCACCTCCG TGCTGCCCAC CCCCAGTGAG GGCCTAAGCA CCTCCGGGCC TCCCACCATC 
TCTAAGGGGC TGTGCACCTC TGTGACGCTT GCCGCCTCTG AGGGCCGGAA CACCTCCAGG 
CCGCCCACTT CCTCTGAGGA ACCTAGCACC TCCGTGCCGC CCACCGCCTC TGAGGTACCG 
AGCACCTCCC TGCCGCCCAC CCCTGGTGAG GGAACGAGCA CCTCCGTGCC GCCCACAGCC 
TATGAGGGAC CAAGCACCTC CGTGGTGCCC ACCCCTGATG AGGGACCAAG CACCTCCGTG 
CTGCCTACAC CTGGTGAGGG ACCAGGCACC TCCGTGCCGC TCGCCGCCAC TGAGGGCCTG 
AGCACCTCCG TGCAGGCCAC TCCTGATGAG GGACCGAGCA CCTCCGTGCC GCCCACCGCC 
ACTGAGGGCC TAAGCACCCC CGTGCCACCC ACCCGTGATG AGGGACCGAG CACCTCCGTG 
CCGGCCACTC CTGGTGAGGG ACCGAGCACC TCCGTGCTGC CCGCCGCCTC TGACGGACAA 
AGCATCTCCT TGGTGCCCAC CCGCGGTAAG GGATCAAGCA CCTCCGTGCC CCCCACCGCC 
ACCGAGGGCC TGAGCACCTC CGTGCAGCCC ACTGCTGGTG AGGGATCGAG CACCTCCGTG 
CCGCCCACCC CTGGTGGGGG ACTGAGCACC TCCGTGCCGC CCACCGCCAC TGAGGAGTTG 
AGCACCTCCG TGCCGCCCAC TCCCGGTGAG GGACCAAGCA CTTCCGTACT GCCAATCCCC 
GGTGAGGGAC TGAGCACCTC TGTGCCGCCC ACCGCCTCTG ATGGATCGGA CACCTCCGTG 
CCGCCCACTC CTGGTGAGGG CGCAAGCACC TTAGTGCAGC CCACCGCCCC TGACGGACCG 
GGAAGCTCCG TGCTGCCTAA CCCTGGTGAG GGCCCGAGCA CATTGTTTAG CTCTAGTGCT 
TCTGTGGACC GGAACCCCTC CAAGTGTTCC CTTGTTTTGC CAAGCCCTAG GGTAACCAAG 
GCCTCCGTGG ACTCAGATTC TGAGGGTCCT AAGGGTGCAG AAGGCCCTAT AGAATTCGAG 
GTCCTGAGAG ACTGTGAGAG CCCCAACTCC ATTAGTATTA TGGGCCTCAA TACTTCCCGG 
GTTGCAATTA CCCTGAAGCC CCAAGACCCT ATGGAACAGA ACGTAGCTGA GCTGTTGCAG 
TTCCTGCTGG TGAAGGATCA GAGCAAGTAC CCTATCCGGG AGTCTGAAAT GCGGGAATAT 
ATTGTTAAAG AATATCGCAA CCAGTTTCCT GAGATACTCA GGCGAGCAGC AGCCCACCTG 
GAGTGCATTT TTAGGTTTGA ATTGAGAGAA CTTGACCCTG AGGCACACAC CTACATTCTG 
TTAAACAAAC TGGGACCTGT GCCCTTTGAA GGGTTAGAAG AGAGCCCAAA TGGGCCAAAG 
ATGGGCCTCC TGATGATGAT TCTAGGCCAA ATATTCCTGA ATGGCAACCA AGCCAAGGAG 
GCTGAGATTT GGGAAATGCT CTGGAGGATG GGGGTGCAGC GGGAAAGGAG GCTTTCCATT 
TTTGGGAACC CAAAGAGACT TCTGTCTGTG GAGTTTGTAT GGCAGCGTTA CTTAGACTAC 
AGGCCAGTAA CTGACTGTAA ACCAGTGGAG TATGAGTTTT TCTGGGGCCC AAGATCCCAC 
CTAGAAACCA CCAAGATGAA AATTCTGAAG TTCATGGCGA AAATATATAA CAAAGATCCT 
ATGGATTGGC CAGAGAAATA CAACGAAGCT CTGGAAGAAG ATGCTGCCAG AGCCTTTGCT 
GAGGGTTGGC AGGCTCTCCC TCACTTTAGG AGGCCCTTTT TTGAGGAAGC TGCTGCAGAG 
GTACCATCCC CTGATTCAGA GGTTTCCAGC TATTCCTCAA AATATGCCCC ACATTCATGG 
CCTGAGTCAA GATTGGAGAG CAAGGCAAGG AAGCTGGTGC AGTTATTTCT GCTTATGGAT 
TCAACTAAGC TGCCTATACC AAAGAAAGGA ATTCTGTACT ACATTGGCCG AGAGTGCAGC 
AAAGTGTTCC CTGACCTCCT GAATCGTGCT GCCCGCACCC TGAACCATGT CTATGGGACA 
GAACTAGTGG TACTTGATCC CAGGAATCAC TCCTATACTC TGTACAACCG AAGGGAGATG 
GAAGAAACTG AGGAGATCGT AGACAGTCCA AACAGGCCTG GCAACAACTT TTTGATGCAG 
GTCCTAAGCT TCATCTTTAT TATGGGCAAC CATGCCAGGG AGTCTGCAGT CTGGGCCTTT 
CTGCGGGGCT TAGGGGTTCA AGCTGGGAGA AAGCATGTGA TTACCTGCAG ATACTTGAGT 
CAGCGCTATA TAGACAGTTT ACGGGTTCCT GACAGTGATC CAGTGCAATA TGAGTTTGTA 
TGGGGTCCTA GAGCCCGTTT GGAAACCTCT AAGATGAAAG CCTTGCGATA TGTGGCCAGA 
ATCCACAGAA AGGAACCACA GGACTGGCCA CAGCAGTACA GGGAGGCAAT GGAAGATGAG 
GCCAATAGAG CTGATGTTGG GCACAGGCAA ATCTTTGTTC ACAACTTCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065983.1
CDS	279..3152
Translation	MSLVSQNSRRRRRRVAKATAHNSSWGEMQAPNAPGLPADVPGSDVPQGPSDSQILQGLCASEGPSTSVLPTSAEGPSTFVPPTISEASSASGQPTISEGPGTSVLPTPSEGLSTSGPPTISKGLCTSVTLAASEGRNTSRPPTSSEEPSTSVPPTASEVPSTSLPPTPGEGTSTSVPPTAYEGPSTSVVPTPDEGPSTSVLPTPGEGPGTSVPLAATEGLSTSVQATPDEGPSTSVPPTATEGLSTPVPPTRDEGPSTSVPATPGEGPSTSVLPAASDGQSISLVPTRGKGSSTSVPPTATEGLSTSVQPTAGEGSSTSVPPTPGGGLSTSVPPTATEELSTSVPPTPGEGPSTSVLPIPGEGLSTSVPPTASDGSDTSVPPTPGEGASTLVQPTAPDGPGSSVLPNPGEGPSTLFSSSASVDRNPSKCSLVLPSPRVTKASVDSDSEGPKGAEGPIEFEVLRDCESPNSISIMGLNTSRVAITLKPQDPMEQNVAELLQFLLVKDQSKYPIRESEMREYIVKEYRNQFPEILRRAAAHLECIFRFELRELDPEAHTYILLNKLGPVPFEGLEESPNGPKMGLLMMILGQIFLNGNQAKEAEIWEMLWRMGVQRERRLSIFGNPKRLLSVEFVWQRYLDYRPVTDCKPVEYEFFWGPRSHLETTKMKILKFMAKIYNKDPMDWPEKYNEALEEDAARAFAEGWQALPHFRRPFFEEAAAEVPSPDSEVSSYSSKYAPHSWPESRLESKARKLVQLFLLMDSTKLPIPKKGILYYIGRECSKVFPDLLNRAARTLNHVYGTELVVLDPRNHSYTLYNRREMEETEEIVDSPNRPGNNFLMQVLSFIFIMGNHARESAVWAFLRGLGVQAGRKHVITCRYLSQRYIDSLRVPDSDPVQYEFVWGPRARLETSKMKALRYVARIHRKEPQDWPQQYREAMEDEANRADVGHRQIFVHNFR

Target ORF information:

RefSeq Version	NM_020932.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member E1 (MAGEE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020932.2

ORF Insert Sequence:

ATGTCTCTGG TAAGCCAGAA TTCGCGCCGC CGCCGCCGCC GCGTTGCAAA GGCTACTGCG 
CACAACAGCA GCTGGGGCGA AATGCAGGCC CCTAATGCCC CCGGTCTCCC CGCTGATGTG 
CCAGGCTCAG ACGTCCCCCA GGGTCCCAGC GATTCCCAGA TCCTCCAGGG CCTCTGCGCC 
TCTGAGGGCC CAAGCACCTC CGTTCTGCCC ACCTCCGCTG AGGGCCCAAG CACCTTTGTG 
CCGCCCACCA TCTCTGAGGC CTCAAGCGCC TCCGGGCAGC CCACCATCTC TGAGGGACCT 
GGCACCTCCG TGCTGCCCAC CCCCAGTGAG GGCCTAAGCA CCTCCGGGCC TCCCACCATC 
TCTAAGGGGC TGTGCACCTC TGTGACGCTT GCCGCCTCTG AGGGCCGGAA CACCTCCAGG 
CCGCCCACTT CCTCTGAGGA ACCTAGCACC TCCGTGCCGC CCACCGCCTC TGAGGTACCG 
AGCACCTCCC TGCCGCCCAC CCCTGGTGAG GGAACGAGCA CCTCCGTGCC GCCCACAGCC 
TATGAGGGAC CAAGCACCTC CGTGGTGCCC ACCCCTGATG AGGGACCAAG CACCTCCGTG 
CTGCCTACAC CTGGTGAGGG ACCAGGCACC TCCGTGCCGC TCGCCGCCAC TGAGGGCCTG 
AGCACCTCCG TGCAGGCCAC TCCTGATGAG GGACCGAGCA CCTCCGTGCC GCCCACCGCC 
ACTGAGGGCC TAAGCACCCC CGTGCCACCC ACCCGTGATG AGGGACCGAG CACCTCCGTG 
CCGGCCACTC CTGGTGAGGG ACCGAGCACC TCCGTGCTGC CCGCCGCCTC TGACGGACAA 
AGCATCTCCT TGGTGCCCAC CCGCGGTAAG GGATCAAGCA CCTCCGTGCC CCCCACCGCC 
ACCGAGGGCC TGAGCACCTC CGTGCAGCCC ACTGCTGGTG AGGGATCGAG CACCTCCGTG 
CCGCCCACCC CTGGTGGGGG ACTGAGCACC TCCGTGCCGC CCACCGCCAC TGAGGAGTTG 
AGCACCTCCG TGCCGCCCAC TCCCGGTGAG GGACCAAGCA CTTCCGTACT GCCAATCCCC 
GGTGAGGGAC TGAGCACCTC TGTGCCGCCC ACCGCCTCTG ATGGATCGGA CACCTCCGTG 
CCGCCCACTC CTGGTGAGGG CGCAAGCACC TTAGTGCAGC CCACCGCCCC TGACGGACCG 
GGAAGCTCCG TGCTGCCTAA CCCTGGTGAG GGCCCGAGCA CATTGTTTAG CTCTAGTGCT 
TCTGTGGACC GGAACCCCTC CAAGTGTTCC CTTGTTTTGC CAAGCCCTAG GGTAACCAAG 
GCCTCCGTGG ACTCAGATTC TGAGGGTCCT AAGGGTGCAG AAGGCCCTAT AGAATTCGAG 
GTCCTGAGAG ACTGTGAGAG CCCCAACTCC ATTAGTATTA TGGGCCTCAA TACTTCCCGG 
GTTGCAATTA CCCTGAAGCC CCAAGACCCT ATGGAACAGA ACGTAGCTGA GCTGTTGCAG 
TTCCTGCTGG TGAAGGATCA GAGCAAGTAC CCTATCCGGG AGTCTGAAAT GCGGGAATAT 
ATTGTTAAAG AATATCGCAA CCAGTTTCCT GAGATACTCA GGCGAGCAGC AGCCCACCTG 
GAGTGCATTT TTAGGTTTGA ATTGAGAGAA CTTGACCCTG AGGCACACAC CTACATTCTG 
TTAAACAAAC TGGGACCTGT GCCCTTTGAA GGGTTAGAAG AGAGCCCAAA TGGGCCAAAG 
ATGGGCCTCC TGATGATGAT TCTAGGCCAA ATATTCCTGA ATGGCAACCA AGCCAAGGAG 
GCTGAGATTT GGGAAATGCT CTGGAGGATG GGGGTGCAGC GGGAAAGGAG GCTTTCCATT 
TTTGGGAACC CAAAGAGACT TCTGTCTGTG GAGTTTGTAT GGCAGCGTTA CTTAGACTAC 
AGGCCAGTAA CTGACTGTAA ACCAGTGGAG TATGAGTTTT TCTGGGGCCC AAGATCCCAC 
CTAGAAACCA CCAAGATGAA AATTCTGAAG TTCATGGCGA AAATATATAA CAAAGATCCT 
ATGGATTGGC CAGAGAAATA CAACGAAGCT CTGGAAGAAG ATGCTGCCAG AGCCTTTGCT 
GAGGGTTGGC AGGCTCTCCC TCACTTTAGG AGGCCCTTTT TTGAGGAAGC TGCTGCAGAG 
GTACCATCCC CTGATTCAGA GGTTTCCAGC TATTCCTCAA AATATGCCCC ACATTCATGG 
CCTGAGTCAA GATTGGAGAG CAAGGCAAGG AAGCTGGTGC AGTTATTTCT GCTTATGGAT 
TCAACTAAGC TGCCTATACC AAAGAAAGGA ATTCTGTACT ACATTGGCCG AGAGTGCAGC 
AAAGTGTTCC CTGACCTCCT GAATCGTGCT GCCCGCACCC TGAACCATGT CTATGGGACA 
GAACTAGTGG TACTTGATCC CAGGAATCAC TCCTATACTC TGTACAACCG AAGGGAGATG 
GAAGAAACTG AGGAGATCGT AGACAGTCCA AACAGGCCTG GCAACAACTT TTTGATGCAG 
GTCCTAAGCT TCATCTTTAT TATGGGCAAC CATGCCAGGG AGTCTGCAGT CTGGGCCTTT 
CTGCGGGGCT TAGGGGTTCA AGCTGGGAGA AAGCATGTGA TTACCTGCAG ATACTTGAGT 
CAGCGCTATA TAGACAGTTT ACGGGTTCCT GACAGTGATC CAGTGCAATA TGAGTTTGTA 
TGGGGTCCTA GAGCCCGTTT GGAAACCTCT AAGATGAAAG CCTTGCGATA TGTGGCCAGA 
ATCCACAGAA AGGAACCACA GGACTGGCCA CAGCAGTACA GGGAGGCAAT GGAAGATGAG 
GCCAATAGAG CTGATGTTGG GCACAGGCAA ATCTTTGTTC ACAACTTCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGEE1 ( NM_020932.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065983.1 Homo sapiens MAGE family member E1 (MAGEE1), mRNA.

Price & Availability↑

CloneID	OHu19574
Clone ID Related Accession (Same CDS sequence)	NM_020932.2 , NM_020932.3
Accession Version	NM_020932.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	melanoma-associated antigen E1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA411859.1 and AB046807.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_020932.2. ##Evidence-Data-START## Transcript is intronless :: SRR1660805.100158.1, SRR1660805.38997.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361470.4/ ENSP00000354912.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGTCTCTGG TAAGCCAGAA TTCGCGCCGC CGCCGCCGCC GCGTTGCAAA GGCTACTGCG 
CACAACAGCA GCTGGGGCGA AATGCAGGCC CCTAATGCCC CCGGTCTCCC CGCTGATGTG 
CCAGGCTCAG ACGTCCCCCA GGGTCCCAGC GATTCCCAGA TCCTCCAGGG CCTCTGCGCC 
TCTGAGGGCC CAAGCACCTC CGTTCTGCCC ACCTCCGCTG AGGGCCCAAG CACCTTTGTG 
CCGCCCACCA TCTCTGAGGC CTCAAGCGCC TCCGGGCAGC CCACCATCTC TGAGGGACCT 
GGCACCTCCG TGCTGCCCAC CCCCAGTGAG GGCCTAAGCA CCTCCGGGCC TCCCACCATC 
TCTAAGGGGC TGTGCACCTC TGTGACGCTT GCCGCCTCTG AGGGCCGGAA CACCTCCAGG 
CCGCCCACTT CCTCTGAGGA ACCTAGCACC TCCGTGCCGC CCACCGCCTC TGAGGTACCG 
AGCACCTCCC TGCCGCCCAC CCCTGGTGAG GGAACGAGCA CCTCCGTGCC GCCCACAGCC 
TATGAGGGAC CAAGCACCTC CGTGGTGCCC ACCCCTGATG AGGGACCAAG CACCTCCGTG 
CTGCCTACAC CTGGTGAGGG ACCAGGCACC TCCGTGCCGC TCGCCGCCAC TGAGGGCCTG 
AGCACCTCCG TGCAGGCCAC TCCTGATGAG GGACCGAGCA CCTCCGTGCC GCCCACCGCC 
ACTGAGGGCC TAAGCACCCC CGTGCCACCC ACCCGTGATG AGGGACCGAG CACCTCCGTG 
CCGGCCACTC CTGGTGAGGG ACCGAGCACC TCCGTGCTGC CCGCCGCCTC TGACGGACAA 
AGCATCTCCT TGGTGCCCAC CCGCGGTAAG GGATCAAGCA CCTCCGTGCC CCCCACCGCC 
ACCGAGGGCC TGAGCACCTC CGTGCAGCCC ACTGCTGGTG AGGGATCGAG CACCTCCGTG 
CCGCCCACCC CTGGTGGGGG ACTGAGCACC TCCGTGCCGC CCACCGCCAC TGAGGAGTTG 
AGCACCTCCG TGCCGCCCAC TCCCGGTGAG GGACCAAGCA CTTCCGTACT GCCAATCCCC 
GGTGAGGGAC TGAGCACCTC TGTGCCGCCC ACCGCCTCTG ATGGATCGGA CACCTCCGTG 
CCGCCCACTC CTGGTGAGGG CGCAAGCACC TTAGTGCAGC CCACCGCCCC TGACGGACCG 
GGAAGCTCCG TGCTGCCTAA CCCTGGTGAG GGCCCGAGCA CATTGTTTAG CTCTAGTGCT 
TCTGTGGACC GGAACCCCTC CAAGTGTTCC CTTGTTTTGC CAAGCCCTAG GGTAACCAAG 
GCCTCCGTGG ACTCAGATTC TGAGGGTCCT AAGGGTGCAG AAGGCCCTAT AGAATTCGAG 
GTCCTGAGAG ACTGTGAGAG CCCCAACTCC ATTAGTATTA TGGGCCTCAA TACTTCCCGG 
GTTGCAATTA CCCTGAAGCC CCAAGACCCT ATGGAACAGA ACGTAGCTGA GCTGTTGCAG 
TTCCTGCTGG TGAAGGATCA GAGCAAGTAC CCTATCCGGG AGTCTGAAAT GCGGGAATAT 
ATTGTTAAAG AATATCGCAA CCAGTTTCCT GAGATACTCA GGCGAGCAGC AGCCCACCTG 
GAGTGCATTT TTAGGTTTGA ATTGAGAGAA CTTGACCCTG AGGCACACAC CTACATTCTG 
TTAAACAAAC TGGGACCTGT GCCCTTTGAA GGGTTAGAAG AGAGCCCAAA TGGGCCAAAG 
ATGGGCCTCC TGATGATGAT TCTAGGCCAA ATATTCCTGA ATGGCAACCA AGCCAAGGAG 
GCTGAGATTT GGGAAATGCT CTGGAGGATG GGGGTGCAGC GGGAAAGGAG GCTTTCCATT 
TTTGGGAACC CAAAGAGACT TCTGTCTGTG GAGTTTGTAT GGCAGCGTTA CTTAGACTAC 
AGGCCAGTAA CTGACTGTAA ACCAGTGGAG TATGAGTTTT TCTGGGGCCC AAGATCCCAC 
CTAGAAACCA CCAAGATGAA AATTCTGAAG TTCATGGCGA AAATATATAA CAAAGATCCT 
ATGGATTGGC CAGAGAAATA CAACGAAGCT CTGGAAGAAG ATGCTGCCAG AGCCTTTGCT 
GAGGGTTGGC AGGCTCTCCC TCACTTTAGG AGGCCCTTTT TTGAGGAAGC TGCTGCAGAG 
GTACCATCCC CTGATTCAGA GGTTTCCAGC TATTCCTCAA AATATGCCCC ACATTCATGG 
CCTGAGTCAA GATTGGAGAG CAAGGCAAGG AAGCTGGTGC AGTTATTTCT GCTTATGGAT 
TCAACTAAGC TGCCTATACC AAAGAAAGGA ATTCTGTACT ACATTGGCCG AGAGTGCAGC 
AAAGTGTTCC CTGACCTCCT GAATCGTGCT GCCCGCACCC TGAACCATGT CTATGGGACA 
GAACTAGTGG TACTTGATCC CAGGAATCAC TCCTATACTC TGTACAACCG AAGGGAGATG 
GAAGAAACTG AGGAGATCGT AGACAGTCCA AACAGGCCTG GCAACAACTT TTTGATGCAG 
GTCCTAAGCT TCATCTTTAT TATGGGCAAC CATGCCAGGG AGTCTGCAGT CTGGGCCTTT 
CTGCGGGGCT TAGGGGTTCA AGCTGGGAGA AAGCATGTGA TTACCTGCAG ATACTTGAGT 
CAGCGCTATA TAGACAGTTT ACGGGTTCCT GACAGTGATC CAGTGCAATA TGAGTTTGTA 
TGGGGTCCTA GAGCCCGTTT GGAAACCTCT AAGATGAAAG CCTTGCGATA TGTGGCCAGA 
ATCCACAGAA AGGAACCACA GGACTGGCCA CAGCAGTACA GGGAGGCAAT GGAAGATGAG 
GCCAATAGAG CTGATGTTGG GCACAGGCAA ATCTTTGTTC ACAACTTCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065983.1
CDS	222..3095
Translation	MSLVSQNSRRRRRRVAKATAHNSSWGEMQAPNAPGLPADVPGSDVPQGPSDSQILQGLCASEGPSTSVLPTSAEGPSTFVPPTISEASSASGQPTISEGPGTSVLPTPSEGLSTSGPPTISKGLCTSVTLAASEGRNTSRPPTSSEEPSTSVPPTASEVPSTSLPPTPGEGTSTSVPPTAYEGPSTSVVPTPDEGPSTSVLPTPGEGPGTSVPLAATEGLSTSVQATPDEGPSTSVPPTATEGLSTPVPPTRDEGPSTSVPATPGEGPSTSVLPAASDGQSISLVPTRGKGSSTSVPPTATEGLSTSVQPTAGEGSSTSVPPTPGGGLSTSVPPTATEELSTSVPPTPGEGPSTSVLPIPGEGLSTSVPPTASDGSDTSVPPTPGEGASTLVQPTAPDGPGSSVLPNPGEGPSTLFSSSASVDRNPSKCSLVLPSPRVTKASVDSDSEGPKGAEGPIEFEVLRDCESPNSISIMGLNTSRVAITLKPQDPMEQNVAELLQFLLVKDQSKYPIRESEMREYIVKEYRNQFPEILRRAAAHLECIFRFELRELDPEAHTYILLNKLGPVPFEGLEESPNGPKMGLLMMILGQIFLNGNQAKEAEIWEMLWRMGVQRERRLSIFGNPKRLLSVEFVWQRYLDYRPVTDCKPVEYEFFWGPRSHLETTKMKILKFMAKIYNKDPMDWPEKYNEALEEDAARAFAEGWQALPHFRRPFFEEAAAEVPSPDSEVSSYSSKYAPHSWPESRLESKARKLVQLFLLMDSTKLPIPKKGILYYIGRECSKVFPDLLNRAARTLNHVYGTELVVLDPRNHSYTLYNRREMEETEEIVDSPNRPGNNFLMQVLSFIFIMGNHARESAVWAFLRGLGVQAGRKHVITCRYLSQRYIDSLRVPDSDPVQYEFVWGPRARLETSKMKALRYVARIHRKEPQDWPQQYREAMEDEANRADVGHRQIFVHNFR

Target ORF information:

RefSeq Version	NM_020932.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member E1 (MAGEE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020932.3

ORF Insert Sequence:

ATGTCTCTGG TAAGCCAGAA TTCGCGCCGC CGCCGCCGCC GCGTTGCAAA GGCTACTGCG 
CACAACAGCA GCTGGGGCGA AATGCAGGCC CCTAATGCCC CCGGTCTCCC CGCTGATGTG 
CCAGGCTCAG ACGTCCCCCA GGGTCCCAGC GATTCCCAGA TCCTCCAGGG CCTCTGCGCC 
TCTGAGGGCC CAAGCACCTC CGTTCTGCCC ACCTCCGCTG AGGGCCCAAG CACCTTTGTG 
CCGCCCACCA TCTCTGAGGC CTCAAGCGCC TCCGGGCAGC CCACCATCTC TGAGGGACCT 
GGCACCTCCG TGCTGCCCAC CCCCAGTGAG GGCCTAAGCA CCTCCGGGCC TCCCACCATC 
TCTAAGGGGC TGTGCACCTC TGTGACGCTT GCCGCCTCTG AGGGCCGGAA CACCTCCAGG 
CCGCCCACTT CCTCTGAGGA ACCTAGCACC TCCGTGCCGC CCACCGCCTC TGAGGTACCG 
AGCACCTCCC TGCCGCCCAC CCCTGGTGAG GGAACGAGCA CCTCCGTGCC GCCCACAGCC 
TATGAGGGAC CAAGCACCTC CGTGGTGCCC ACCCCTGATG AGGGACCAAG CACCTCCGTG 
CTGCCTACAC CTGGTGAGGG ACCAGGCACC TCCGTGCCGC TCGCCGCCAC TGAGGGCCTG 
AGCACCTCCG TGCAGGCCAC TCCTGATGAG GGACCGAGCA CCTCCGTGCC GCCCACCGCC 
ACTGAGGGCC TAAGCACCCC CGTGCCACCC ACCCGTGATG AGGGACCGAG CACCTCCGTG 
CCGGCCACTC CTGGTGAGGG ACCGAGCACC TCCGTGCTGC CCGCCGCCTC TGACGGACAA 
AGCATCTCCT TGGTGCCCAC CCGCGGTAAG GGATCAAGCA CCTCCGTGCC CCCCACCGCC 
ACCGAGGGCC TGAGCACCTC CGTGCAGCCC ACTGCTGGTG AGGGATCGAG CACCTCCGTG 
CCGCCCACCC CTGGTGGGGG ACTGAGCACC TCCGTGCCGC CCACCGCCAC TGAGGAGTTG 
AGCACCTCCG TGCCGCCCAC TCCCGGTGAG GGACCAAGCA CTTCCGTACT GCCAATCCCC 
GGTGAGGGAC TGAGCACCTC TGTGCCGCCC ACCGCCTCTG ATGGATCGGA CACCTCCGTG 
CCGCCCACTC CTGGTGAGGG CGCAAGCACC TTAGTGCAGC CCACCGCCCC TGACGGACCG 
GGAAGCTCCG TGCTGCCTAA CCCTGGTGAG GGCCCGAGCA CATTGTTTAG CTCTAGTGCT 
TCTGTGGACC GGAACCCCTC CAAGTGTTCC CTTGTTTTGC CAAGCCCTAG GGTAACCAAG 
GCCTCCGTGG ACTCAGATTC TGAGGGTCCT AAGGGTGCAG AAGGCCCTAT AGAATTCGAG 
GTCCTGAGAG ACTGTGAGAG CCCCAACTCC ATTAGTATTA TGGGCCTCAA TACTTCCCGG 
GTTGCAATTA CCCTGAAGCC CCAAGACCCT ATGGAACAGA ACGTAGCTGA GCTGTTGCAG 
TTCCTGCTGG TGAAGGATCA GAGCAAGTAC CCTATCCGGG AGTCTGAAAT GCGGGAATAT 
ATTGTTAAAG AATATCGCAA CCAGTTTCCT GAGATACTCA GGCGAGCAGC AGCCCACCTG 
GAGTGCATTT TTAGGTTTGA ATTGAGAGAA CTTGACCCTG AGGCACACAC CTACATTCTG 
TTAAACAAAC TGGGACCTGT GCCCTTTGAA GGGTTAGAAG AGAGCCCAAA TGGGCCAAAG 
ATGGGCCTCC TGATGATGAT TCTAGGCCAA ATATTCCTGA ATGGCAACCA AGCCAAGGAG 
GCTGAGATTT GGGAAATGCT CTGGAGGATG GGGGTGCAGC GGGAAAGGAG GCTTTCCATT 
TTTGGGAACC CAAAGAGACT TCTGTCTGTG GAGTTTGTAT GGCAGCGTTA CTTAGACTAC 
AGGCCAGTAA CTGACTGTAA ACCAGTGGAG TATGAGTTTT TCTGGGGCCC AAGATCCCAC 
CTAGAAACCA CCAAGATGAA AATTCTGAAG TTCATGGCGA AAATATATAA CAAAGATCCT 
ATGGATTGGC CAGAGAAATA CAACGAAGCT CTGGAAGAAG ATGCTGCCAG AGCCTTTGCT 
GAGGGTTGGC AGGCTCTCCC TCACTTTAGG AGGCCCTTTT TTGAGGAAGC TGCTGCAGAG 
GTACCATCCC CTGATTCAGA GGTTTCCAGC TATTCCTCAA AATATGCCCC ACATTCATGG 
CCTGAGTCAA GATTGGAGAG CAAGGCAAGG AAGCTGGTGC AGTTATTTCT GCTTATGGAT 
TCAACTAAGC TGCCTATACC AAAGAAAGGA ATTCTGTACT ACATTGGCCG AGAGTGCAGC 
AAAGTGTTCC CTGACCTCCT GAATCGTGCT GCCCGCACCC TGAACCATGT CTATGGGACA 
GAACTAGTGG TACTTGATCC CAGGAATCAC TCCTATACTC TGTACAACCG AAGGGAGATG 
GAAGAAACTG AGGAGATCGT AGACAGTCCA AACAGGCCTG GCAACAACTT TTTGATGCAG 
GTCCTAAGCT TCATCTTTAT TATGGGCAAC CATGCCAGGG AGTCTGCAGT CTGGGCCTTT 
CTGCGGGGCT TAGGGGTTCA AGCTGGGAGA AAGCATGTGA TTACCTGCAG ATACTTGAGT 
CAGCGCTATA TAGACAGTTT ACGGGTTCCT GACAGTGATC CAGTGCAATA TGAGTTTGTA 
TGGGGTCCTA GAGCCCGTTT GGAAACCTCT AAGATGAAAG CCTTGCGATA TGTGGCCAGA 
ATCCACAGAA AGGAACCACA GGACTGGCCA CAGCAGTACA GGGAGGCAAT GGAAGATGAG 
GCCAATAGAG CTGATGTTGG GCACAGGCAA ATCTTTGTTC ACAACTTCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Naturally occurring HCA1 missense mutations result in loss of function: potential impact on lipid deposition.
Journal of lipid research54(3)823-30(2013 Mar)
Doyle JR,Lane JM,Beinborn M,Kopin AS

MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases.
Molecular cell39(6)963-74(2010 Sep)
Doyle JM,Gao J,Wang J,Yang M,Potts PR

Frequent MAGE mutations in human melanoma.
PloS one5(9)(2010 Sep)
Caballero OL,Zhao Q,Rimoldi D,Stevenson BJ,Svobodov? S,Devalle S,R?hrig UF,Pagotto A,Michielin O,Speiser D,Wolchok JD,Liu C,Pejovic T,Odunsi K,Brasseur F,Van den Eynde BJ,Old LJ,Lu X,Cebon J,Strausberg RL,Simpson AJ

DAMAGE, a novel alpha-dystrobrevin-associated MAGE protein in dystrophin complexes.
The Journal of biological chemistry279(8)7014-23(2004 Feb)
Albrecht DE,Froehner SC