DAZ3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DAZ3
Entrez Gene ID	57054
Full Name	deleted in azoospermia 3
Synonyms	pDP1679
General protein information	Preferred Names deleted in azoospermia 3 Names deleted in azoospermia protein 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: Y Map Location: Yq11.23
Summary	This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains one copy of the 10.8 kb repeat. [provided by RefSeq, Jul 2008].
Disorder MIM:	400027

mRNA and Protein(s)

mRNA	Protein	Name
NM_020364.3	NP_065097.2	deleted in azoospermia protein 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	DAZ3	NP_065097.2
Pan troglodytes (chimpanzee)	DAZ3	XP_001138549.3

Gene Ontology
Bibliography

Related articles in PubMed

Impact of partial DAZ1/2 deletion and partial DAZ3/4 deletion on male infertility.
Zhang Y, Li M, Xiao F, Teng R, Zhang C, Lan A, Gu K, Li J, Wang D, Li H, Jiang L, Zeng S, He M, Huang Y, Guo P, Zhang X, Yang X
Gene571(1)9-16(2015 Oct)

Polymorphic expression of DAZ proteins in the human testis.
Kim B, Lee Y, Kim Y, Lee KH, Chun S, Rhee K, Seo JT, Kim SW, Paick JS
Human reproduction (Oxford, England)24(6)1507-15(2009 Jun)

AZFc partial deletions in Chilean men with severe spermatogenic failure.
Lardone MC, Parodi DA, Ebensperger M, Pe?aloza P, Cornejo V, Valdevenito R, Pommer R, Castro A
Fertility and sterility88(5)1318-26(2007 Nov)

[Study on DAZ gene copy deletion in severe oligozoospermia sperm donor for ICSI].
A ZC, Yang Y, Zhang SZ, Lin L
Yi chuan = Hereditas28(9)1057-60(2006 Sep)

Expression profiles of the DAZ gene family in human testis with and without spermatogenic failure.
Kuo PL, Wang ST, Lin YM, Lin YH, Teng YN, Hsu CC
Fertility and sterility81(4)1034-40(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate that partial RNA-binding proteins DAZ1/2 deletion was associated wih male infertility, but partial RNA-binding proteins DAZ3/4 deletion was not associated with male infertility.
Title: Impact of partial DAZ1/2 deletion and partial DAZ3/4 deletion on male infertility.

All four DAZ genes are expressed in the human testis, and their products are highly polymorphic among men
Title: Polymorphic expression of DAZ proteins in the human testis.

AZFc subdeletions do not seem to cause severe impairment of spermatogenesis in Chilean men.
Title: AZFc partial deletions in Chilean men with severe spermatogenic failure.

Observational study of gene-disease association. (HuGE Navigator)
Title: [Study on DAZ gene copy deletion in severe oligozoospermia sperm donor for ICSI].

a common variant of the human Y chromosome lacks the DAZ3/DAZ4 and BPY2.2/BPY2.3 doublets in distal AZFc and thus these genes cannot be required for male fertility
Title: A large AZFc deletion removes DAZ3/DAZ4 and nearby genes from men in Y haplogroup N.

The following DAZ3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAZ3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu22295	NM_020364.3 Latest version!	Homo sapiens deleted in azoospermia 3 (DAZ3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DAZ3 ( NM_020364.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065097.2 Homo sapiens deleted in azoospermia 3 (DAZ3), mRNA.

Price & Availability↑

CloneID	OHu22295
Clone ID Related Accession (Same CDS sequence)	NM_020364.3
Accession Version	NM_020364.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	deleted in azoospermia protein 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010089.4 and BC113005.1. This sequence is a reference standard in the RefSeqGene project. On Feb 10, 2011 this sequence version replaced NM_020364.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC113005.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142586 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCTG CAAATCCTGA GACTCCAAAC TCAACCATCT CCAGAGAGGC CAGCACCCAG 
TCTTCATCAG CTGCAGCTAG CCAAGGCTGG GTGTTACCAG AAGGCAAAAT CGTGCCAAAC 
ACTGTTTTTG TTGGTGGAAT TGATGCTAGG ATGGATGAAA CTGAGATTGG AAGCTGCTTT 
GGTAGATACG GTTCAGTGAA AGAAGTGAAG ATAATCACGA ATCGAACTGG TGTGTCCAAA 
GGCTATGGAT TTGTTTCGTT TGTTAATGAC GTGGATGTCC AGAAGATAGT AGGATCACAG 
ATACATTTCC ATGGTAAAAA GCTGAAGCTG GGCCCTGCAA TCAGGAAACA AAAGTTATGT 
GCTCGTCATG TGCAGCCACG TCCTTTGGTA GTTAATCCTC CTCCTCCACC ACAGTTTCAG 
AACGTCTGGC GGAATCCAAA CACTGAAACC TACCTGCAGC CCCAAATCAC GCCGAATCCT 
GTAACTCAGC ACGTTCAGGC TTACTCTGCT TATCCACATT CACCAGGTCA GGTCATCACT 
GGATGTCAGT TGCTTGTATA TAATTATCAG GAATATCCTA CTTATCCCGA TTCAGCATTT 
CAGGTCACCA CTGGATATCA GTTGCCTGTA TATAATTATC AGCCATTTCC TGCTTATCCA 
AGATCACCAT TTCAGGTCAC TGCTGGATAT CAGTTGCCTG TATATAATTA TCAGGCATTT 
CCTGCTTATC CAAATTCACC ATTTCAAGTC GCCACTGGAT ATCAGTTCCC TGTATACAAT 
TATCAGGCAT TTCCTGCTTA TCCAAATTCA CCAGTTCAGG TCACCACTGG ATATCAGTTG 
CCTGTATACA ATTATCAGGC ATTTCCTGCT TATCCAAATT CACCATTTCA AGTCGCCACT 
GGATATCAGT TCCCTGTATA CAATTATCAG GCATTTCCTG CTTATCCAAA TTCACCAGTT 
CAGGTCACCA CTGGATATCA GTTGCCTGTA TACAATTATC AGGCATTTCC TGCTTATCCA 
AATTCACCAT TTCAAGTCGC CACTGGATAT CAGTTCCCTG TATACAATTA TCAGGCATTT 
CCTGCTTATC CAAATTCACC AGTTCAGGTC ACCACTGGAT ATCAGTTGCC TGTATACAAT 
TATCAGGCAT TTCCTGCTTA TCCAAATTCA GCAGTTCAGG TCACCACTGG ATATCAGTTC 
CATGTATACA ATTACCAGAT GCCACCGCAG TGCCCTGTTG GGGAGCAAAG GAGAAATCTG 
TGGACCGAAG CATACAAATG GTGGTATCTT GTCTGTTTAA TCCAGAGAAG AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065097.2
CDS	308..1624
Translation	MSAANPETPNSTISREASTQSSSAAASQGWVLPEGKIVPNTVFVGGIDARMDETEIGSCFGRYGSVKEVKIITNRTGVSKGYGFVSFVNDVDVQKIVGSQIHFHGKKLKLGPAIRKQKLCARHVQPRPLVVNPPPPPQFQNVWRNPNTETYLQPQITPNPVTQHVQAYSAYPHSPGQVITGCQLLVYNYQEYPTYPDSAFQVTTGYQLPVYNYQPFPAYPRSPFQVTAGYQLPVYNYQAFPAYPNSPFQVATGYQFPVYNYQAFPAYPNSPVQVTTGYQLPVYNYQAFPAYPNSPFQVATGYQFPVYNYQAFPAYPNSPVQVTTGYQLPVYNYQAFPAYPNSPFQVATGYQFPVYNYQAFPAYPNSPVQVTTGYQLPVYNYQAFPAYPNSAVQVTTGYQFHVYNYQMPPQCPVGEQRRNLWTEAYKWWYLVCLIQRRD

Target ORF information:

RefSeq Version	NM_020364.3
Organism	Homo sapiens(human)
Definition	Homo sapiens deleted in azoospermia 3 (DAZ3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020364.3

ORF Insert Sequence:

ATGTCTGCTG CAAATCCTGA GACTCCAAAC TCAACCATCT CCAGAGAGGC CAGCACCCAG 
TCTTCATCAG CTGCAGCTAG CCAAGGCTGG GTGTTACCAG AAGGCAAAAT CGTGCCAAAC 
ACTGTTTTTG TTGGTGGAAT TGATGCTAGG ATGGATGAAA CTGAGATTGG AAGCTGCTTT 
GGTAGATACG GTTCAGTGAA AGAAGTGAAG ATAATCACGA ATCGAACTGG TGTGTCCAAA 
GGCTATGGAT TTGTTTCGTT TGTTAATGAC GTGGATGTCC AGAAGATAGT AGGATCACAG 
ATACATTTCC ATGGTAAAAA GCTGAAGCTG GGCCCTGCAA TCAGGAAACA AAAGTTATGT 
GCTCGTCATG TGCAGCCACG TCCTTTGGTA GTTAATCCTC CTCCTCCACC ACAGTTTCAG 
AACGTCTGGC GGAATCCAAA CACTGAAACC TACCTGCAGC CCCAAATCAC GCCGAATCCT 
GTAACTCAGC ACGTTCAGGC TTACTCTGCT TATCCACATT CACCAGGTCA GGTCATCACT 
GGATGTCAGT TGCTTGTATA TAATTATCAG GAATATCCTA CTTATCCCGA TTCAGCATTT 
CAGGTCACCA CTGGATATCA GTTGCCTGTA TATAATTATC AGCCATTTCC TGCTTATCCA 
AGATCACCAT TTCAGGTCAC TGCTGGATAT CAGTTGCCTG TATATAATTA TCAGGCATTT 
CCTGCTTATC CAAATTCACC ATTTCAAGTC GCCACTGGAT ATCAGTTCCC TGTATACAAT 
TATCAGGCAT TTCCTGCTTA TCCAAATTCA CCAGTTCAGG TCACCACTGG ATATCAGTTG 
CCTGTATACA ATTATCAGGC ATTTCCTGCT TATCCAAATT CACCATTTCA AGTCGCCACT 
GGATATCAGT TCCCTGTATA CAATTATCAG GCATTTCCTG CTTATCCAAA TTCACCAGTT 
CAGGTCACCA CTGGATATCA GTTGCCTGTA TACAATTATC AGGCATTTCC TGCTTATCCA 
AATTCACCAT TTCAAGTCGC CACTGGATAT CAGTTCCCTG TATACAATTA TCAGGCATTT 
CCTGCTTATC CAAATTCACC AGTTCAGGTC ACCACTGGAT ATCAGTTGCC TGTATACAAT 
TATCAGGCAT TTCCTGCTTA TCCAAATTCA GCAGTTCAGG TCACCACTGG ATATCAGTTC 
CATGTATACA ATTACCAGAT GCCACCGCAG TGCCCTGTTG GGGAGCAAAG GAGAAATCTG 
TGGACCGAAG CATACAAATG GTGGTATCTT GTCTGTTTAA TCCAGAGAAG AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impact of partial DAZ1/2 deletion and partial DAZ3/4 deletion on male infertility.
Gene571(1)9-16(2015 Oct)
Zhang Y,Li M,Xiao F,Teng R,Zhang C,Lan A,Gu K,Li J,Wang D,Li H,Jiang L,Zeng S,He M,Huang Y,Guo P,Zhang X,Yang X

Polymorphic expression of DAZ proteins in the human testis.
Human reproduction (Oxford, England)24(6)1507-15(2009 Jun)
Kim B,Lee Y,Kim Y,Lee KH,Chun S,Rhee K,Seo JT,Kim SW,Paick JS

AZFc partial deletions in Chilean men with severe spermatogenic failure.
Fertility and sterility88(5)1318-26(2007 Nov)
Lardone MC,Parodi DA,Ebensperger M,Pe?aloza P,Cornejo V,Valdevenito R,Pommer R,Castro A

[Study on DAZ gene copy deletion in severe oligozoospermia sperm donor for ICSI].
Yi chuan = Hereditas28(9)1057-60(2006 Sep)
A ZC,Yang Y,Zhang SZ,Lin L

Expression profiles of the DAZ gene family in human testis with and without spermatogenic failure.
Fertility and sterility81(4)1034-40(2004 Apr)
Kuo PL,Wang ST,Lin YM,Lin YH,Teng YN,Hsu CC