PPP3R1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP3R1
Entrez Gene ID	5534
Full Name	protein phosphatase 3 regulatory subunit B, alpha
Synonyms	CALNB1,CNB,CNB1
General protein information	Preferred Names protein phosphatase 3 regulatory subunit B, alpha Names calcineurin subunit B type 1 calcineurin B, type I (19kDa) protein phosphatase 2B regulatory subunit 1 protein phosphatase 2B regulatory subunit B alpha protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I) protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p14
Disorder MIM:	601302

mRNA and Protein(s)

mRNA	Protein	Name
NM_000945.3	NP_000936.1	calcineurin subunit B type 1
NM_000945.4	NP_000936.1	calcineurin subunit B type 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPP3R1	XP_855383.3
Magnaporthe oryzae (rice blast fungus)	MGG_06933	XP_003709672.1
Neurospora crassa	NCU03833	XP_960592.2
Pan troglodytes (chimpanzee)	PPP3R1	XP_003309091.1
Mus musculus (house mouse)	Ppp3r1	NP_077779.2
Rattus norvegicus (Norway rat)	Ppp3r1	NP_059005.1
Eremothecium gossypii	AGOS_AER096C	NP_984956.1
Schizosaccharomyces pombe (fission yeast)	SPCC830.06	NP_588476.1
	LOC100428682	XP_002799342.1
Bos taurus (cattle)	PPP3R1	NP_777008.1
Gallus gallus (chicken)	PPP3R1	NP_989707.1
Drosophila melanogaster (fruit fly)	CanB	NP_524741.1
Arabidopsis thaliana (thale cress)	AT3G18430	NP_001189924.1
Oryza sativa (rice)	Os08g0442300	NP_001061916.2
Saccharomyces cerevisiae (baker's yeast)	CNB1	NP_012731.1
Xenopus tropicalis (tropical clawed frog)	ppp3r1	NP_989400.1
Homo sapiens (human)	PPP3R1	NP_000936.1
Danio rerio (zebrafish)	ppp3r1a	XP_692770.2
Drosophila melanogaster (fruit fly)	CanB2	NP_524874.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004298	XP_313573.5
Caenorhabditis elegans (roundworm)	cnb-1	NP_001256317.1
Kluyveromyces lactis	KLLA0D02992g	XP_453201.1

Gene Ontology
Bibliography

Related articles in PubMed

MiR-548a-3p Promotes Keratinocyte Proliferation Targeting PPP3R1 after Being Induced by IL-22.
Zhao X, Li R, Qiao M, Yan J, Sun Q
Inflammation(2017 Nov)

Association of CnB 5I/5D promoter gene polymorphism and serum calcineurin levels in early onset of coronary artery disease of south Indian cohort.
Maddhuri S, Bandaru S, Bhukya C, Cingeetham V, Malempati AR, Deepika MLN, Nallari P, Mundluru HP
Gene6321-6(2017 Oct)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Investigating the human Calcineurin Interaction Network using the ??LxVP SLiM.
Sheftic SR, Page R, Peti W
Scientific reports638920(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The CnB 5I/5D variation was found to be significantly associated with CAD. The 5-base pair deletion results in increased serum calcineurin levels.
Title: Association of CnB 5I/5D promoter gene polymorphism and serum calcineurin levels in early onset of coronary artery disease of south Indian cohort.

we report that exogenous CnB is taken up by cells in a time- and concentration-dependent manner via clathrin-dependent receptor-mediated internalization. Our findings further confirm that uptake is mediated by the TLR4/MD2 complex together with the co-receptor CD14
Title: Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4.

CnB is a novel host-derived immunostimulatory factor, having a role as an agonist in monocytes, and specificity in TLR4 signaling through TRAF3 and TRAF6, in response to various agonists.
Title: Calcineurin B stimulates cytokine production through a CD14-independent Toll-like receptor 4 pathway.

The mRNA expressions of PPP3CB and MEF2C were significantly up-regulated, and CAMK1 and PPP3R1 were significantly down-regulated in mitral regurgitation(MR) patients compared to normal subjects. Moreover, MR patients had significantly increased mRNA levels of PPP3CB, MEF2C and PLCE1 compared to aortic valve disease patients
Title: Exploring Regulatory Mechanisms of Atrial Myocyte Hypertrophy of Mitral Regurgitation through Gene Expression Profiling Analysis: Role of NFAT in Cardiac Hypertrophy.

Data (including data from micro-dissected trophoblasts and trophoblastic BeWo cell line) suggest that microRNA-512-3p participates in placental function by targeting PPP3R1, encoding a regulatory subunit of calcineurin B.
Title: Placenta-specific miRNA (miR-512-3p) targets PPP3R1 encoding the calcineurin B regulatory subunit in BeWo cells.

The following PPP3R1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP3R1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02713	NM_000945.4 Latest version!	Homo sapiens protein phosphatase 3 regulatory subunit B, alpha (PPP3R1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu02713	NM_000945.3	Homo sapiens protein phosphatase 3 regulatory subunit B, alpha (PPP3R1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP3R1 ( NM_000945.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000936.1 Homo sapiens protein phosphatase 3 regulatory subunit B, alpha (PPP3R1), mRNA.

Price & Availability↑

CloneID	OHu02713
Clone ID Related Accession (Same CDS sequence)	NM_000945.3 , NM_000945.4
Accession Version	NM_000945.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-25
Organism	Homo sapiens(human)
Product	calcineurin subunit B type 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM468705.1, BC027913.1 and BQ228635.1. On Mar 8, 2004 this sequence version replaced NM_000945.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027913.1, SRR1163655.39828.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGGAAATG AGGCAAGTTA TCCTTTGGAA ATGTGCTCAC ACTTTGATGC GGATGAAATT 
AAAAGGCTAG GAAAGAGATT TAAGAAGCTT GATTTGGACA ATTCTGGTTC TTTGAGTGTG 
GAAGAGTTCA TGTCTCTGCC TGAGTTACAA CAGAATCCTT TAGTACAGCG AGTAATAGAT 
ATATTCGACA CAGATGGGAA TGGAGAAGTA GACTTTAAAG AATTCATTGA GGGCGTCTCT 
CAGTTCAGTG TCAAAGGAGA TAAGGAGCAG AAATTGAGGT TTGCTTTCCG TATCTATGAC 
ATGGATAAAG ATGGCTATAT TTCCAATGGG GAACTCTTCC AGGTATTGAA GATGATGGTG 
GGGAACAATC TGAAAGATAC ACAGTTACAG CAAATTGTAG ACAAAACCAT AATAAATGCA 
GATAAGGATG GAGATGGAAG AATATCCTTT GAAGAATTCT GTGCTGTTGT AGGTGGCCTA 
GATATCCACA AAAAGATGGT GGTAGATGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000936.1
CDS	393..905
Translation	MGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDV

Target ORF information:

RefSeq Version	NM_000945.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 3 regulatory subunit B, alpha (PPP3R1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000945.3

ORF Insert Sequence:

ATGGGAAATG AGGCAAGTTA TCCTTTGGAA ATGTGCTCAC ACTTTGATGC GGATGAAATT 
AAAAGGCTAG GAAAGAGATT TAAGAAGCTT GATTTGGACA ATTCTGGTTC TTTGAGTGTG 
GAAGAGTTCA TGTCTCTGCC TGAGTTACAA CAGAATCCTT TAGTACAGCG AGTAATAGAT 
ATATTCGACA CAGATGGGAA TGGAGAAGTA GACTTTAAAG AATTCATTGA GGGCGTCTCT 
CAGTTCAGTG TCAAAGGAGA TAAGGAGCAG AAATTGAGGT TTGCTTTCCG TATCTATGAC 
ATGGATAAAG ATGGCTATAT TTCCAATGGG GAACTCTTCC AGGTATTGAA GATGATGGTG 
GGGAACAATC TGAAAGATAC ACAGTTACAG CAAATTGTAG ACAAAACCAT AATAAATGCA 
GATAAGGATG GAGATGGAAG AATATCCTTT GAAGAATTCT GTGCTGTTGT AGGTGGCCTA 
GATATCCACA AAAAGATGGT GGTAGATGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP3R1 ( NM_000945.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000936.1 Homo sapiens protein phosphatase 3 regulatory subunit B, alpha (PPP3R1), mRNA.

Price & Availability↑

CloneID	OHu02713
Clone ID Related Accession (Same CDS sequence)	NM_000945.3 , NM_000945.4
Accession Version	NM_000945.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	calcineurin subunit B type 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC017083.7, BM468705.1, BC027913.1 and BQ228635.1. On Nov 22, 2018 this sequence version replaced NM_000945.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027913.1, SRR1163655.39828.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000234310.8/ ENSP00000234310.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGAAATG AGGCAAGTTA TCCTTTGGAA ATGTGCTCAC ACTTTGATGC GGATGAAATT 
AAAAGGCTAG GAAAGAGATT TAAGAAGCTT GATTTGGACA ATTCTGGTTC TTTGAGTGTG 
GAAGAGTTCA TGTCTCTGCC TGAGTTACAA CAGAATCCTT TAGTACAGCG AGTAATAGAT 
ATATTCGACA CAGATGGGAA TGGAGAAGTA GACTTTAAAG AATTCATTGA GGGCGTCTCT 
CAGTTCAGTG TCAAAGGAGA TAAGGAGCAG AAATTGAGGT TTGCTTTCCG TATCTATGAC 
ATGGATAAAG ATGGCTATAT TTCCAATGGG GAACTCTTCC AGGTATTGAA GATGATGGTG 
GGGAACAATC TGAAAGATAC ACAGTTACAG CAAATTGTAG ACAAAACCAT AATAAATGCA 
GATAAGGATG GAGATGGAAG AATATCCTTT GAAGAATTCT GTGCTGTTGT AGGTGGCCTA 
GATATCCACA AAAAGATGGT GGTAGATGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000936.1
CDS	406..918
Translation	MGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDV

Target ORF information:

RefSeq Version	NM_000945.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 3 regulatory subunit B, alpha (PPP3R1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000945.4

ORF Insert Sequence:

ATGGGAAATG AGGCAAGTTA TCCTTTGGAA ATGTGCTCAC ACTTTGATGC GGATGAAATT 
AAAAGGCTAG GAAAGAGATT TAAGAAGCTT GATTTGGACA ATTCTGGTTC TTTGAGTGTG 
GAAGAGTTCA TGTCTCTGCC TGAGTTACAA CAGAATCCTT TAGTACAGCG AGTAATAGAT 
ATATTCGACA CAGATGGGAA TGGAGAAGTA GACTTTAAAG AATTCATTGA GGGCGTCTCT 
CAGTTCAGTG TCAAAGGAGA TAAGGAGCAG AAATTGAGGT TTGCTTTCCG TATCTATGAC 
ATGGATAAAG ATGGCTATAT TTCCAATGGG GAACTCTTCC AGGTATTGAA GATGATGGTG 
GGGAACAATC TGAAAGATAC ACAGTTACAG CAAATTGTAG ACAAAACCAT AATAAATGCA 
GATAAGGATG GAGATGGAAG AATATCCTTT GAAGAATTCT GTGCTGTTGT AGGTGGCCTA 
GATATCCACA AAAAGATGGT GGTAGATGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MiR-548a-3p Promotes Keratinocyte Proliferation Targeting PPP3R1 after Being Induced by IL-22.
Inflammation(2017 Nov)
Zhao X,Li R,Qiao M,Yan J,Sun Q

Association of CnB 5I/5D promoter gene polymorphism and serum calcineurin levels in early onset of coronary artery disease of south Indian cohort.
Gene6321-6(2017 Oct)
Maddhuri S,Bandaru S,Bhukya C,Cingeetham V,Malempati AR,Deepika MLN,Nallari P,Mundluru HP

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Investigating the human Calcineurin Interaction Network using the ??LxVP SLiM.
Scientific reports638920(2016 Dec)
Sheftic SR,Page R,Peti W