LEPROT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LEPROT
Entrez Gene ID	54741
Full Name	leptin receptor overlapping transcript
Synonyms	LEPR,OB-RGRP,OBRGRP,VPS55
General protein information	Preferred Names leptin receptor overlapping transcript Names leptin receptor gene-related protein DnaJ (Hsp40) homolog, subfamily C, member 6 OB-R gene-related protein endospanin endospanin-1 leptin receptor overlapping transcript protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p31.3
Summary	LEPROT is associated with the Golgi complex and endosomes and has a role in cell surface expression of growth hormone receptor (GHR; MIM 600946) and leptin receptor (OBR, or LEPR; MIM 601007), thereby altering receptor-mediated cell signaling (Couturier et al., 2007 [PubMed 18042720]; Touvier et al., 2009 [PubMed 19907080]).[supplied by OMIM, Jul 2010].
Disorder MIM:	613461

mRNA and Protein(s)

mRNA	Protein	Name
NM_017526.4	NP_059996.1	leptin receptor gene-related protein isoform 1
NM_017526.5	NP_059996.1	leptin receptor gene-related protein isoform 1
NM_001198683.1	NP_001185612.1	leptin receptor gene-related protein isoform 3
NM_001198681.1	NP_001185610.1	leptin receptor gene-related protein isoform 2
NM_001198683.2	NP_001185612.1	leptin receptor gene-related protein isoform 3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	leprot	NP_001017787.1
Xenopus tropicalis (tropical clawed frog)	leprot	NP_001017102.1
Bos taurus (cattle)	LEPROT	NP_001029913.1
Mus musculus (house mouse)	Leprot	NP_778201.1
Homo sapiens (human)	LEPROT	NP_059996.1
Gallus gallus (chicken)	LEPROT	NP_001007959.1
Canis lupus familiaris (dog)	LOC609115	XP_852098.1
Rattus norvegicus (Norway rat)	Leprot	NP_064484.1

Gene Ontology
Bibliography

Related articles in PubMed

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang C, Heman-Ackah SM, Hessa T, Guha R, Martin SE, Youle RJ
Nature504(7479)291-5(2013 Dec)

Endospanins regulate a postinternalization step of the leptin receptor endocytic pathway.
S?ron K, Couturier C, Belouzard S, Bacart J, Mont? D, Corset L, Bocquet O, Dam J, Vauthier V, Lec?ur C, Bailleul B, Hoflack B, Froguel P, Jockers R, Rouill? Y
The Journal of biological chemistry286(20)17968-81(2011 May)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

Copy number variation at leptin receptor gene locus associated with metabolic traits and the risk of type 2 diabetes mellitus.
Jeon JP, Shim SM, Nam HY, Ryu GM, Hong EJ, Kim HL, Han BG
BMC genomics11426(2010 Jul)

Identification of type 2 diabetes-associated combination of SNPs using support vector machine.
Ban HJ, Heo JY, Oh KS, Park KJ
BMC genetics1126(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Copy number variation at leptin receptor gene locus associated with metabolic traits and the risk of type 2 diabetes mellitus.

Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
Title: Identification of type 2 diabetes-associated combination of SNPs using support vector machine.

Transgenic mice expressing either human LEPROT or human LEPROTL1 displayed growth retardation, reduced plasma IGF1 levels, and impaired hepatic sensitivity to GH, as measured by STAT5 phosphorylation and Socs2 mRNA expression.
Title: LEPROT and LEPROTL1 cooperatively decrease hepatic growth hormone action in mice.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following LEPROT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LEPROT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32203	NM_017526.5 Latest version!	Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu05774	NM_001198683.2 Latest version!	Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu08389	NM_001198681.1 Latest version!	Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu32203	NM_017526.4	Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu05774	NM_001198683.1	Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LEPROT ( NM_017526.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_059996.1 Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu32203
Clone ID Related Accession (Same CDS sequence)	NM_017526.4 , NM_017526.5
Accession Version	NM_017526.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	leptin receptor gene-related protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC140007.1, Y12670.1, AC119800.2 and CA434795.1. On Nov 3, 2010 this sequence version replaced NM_017526.3. Transcript Variant: This variant (1) is dominant and encodes the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.233138.1, SRR1660805.56356.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGCG TTAAAGCTCT CGTGGCATTA TCCTTCAGTG GGGCTATTGG ACTGACTTTT 
CTTATGCTGG GATGTGCCTT AGAGGATTAT GGCGTTTACT GGCCCTTATT CGTCCTGATT 
TTCCACGCCA TCTCCCCCAT CCCCCATTTC ATTGCCAAAA GAGTCACCTA TGACTCAGAT 
GCAACCAGTA GTGCCTGTCG GGAACTGGCA TATTTCTTCA CTACTGGAAT TGTTGTTTCT 
GCCTTTGGAT TTCCTGTTAT TCTTGCTCGT GTGGCTGTGA TCAAATGGGG AGCCTGCGGC 
CTTGTGTTGG CAGGCAATGC AGTCATTTTC CTTACAATTC AAGGGTTTTT CCTTATATTT 
GGAAGAGGAG ATGATTTTAG CTGGGAGCAG TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059996.1
CDS	278..673
Translation	MAGVKALVALSFSGAIGLTFLMLGCALEDYGVYWPLFVLIFHAISPIPHFIAKRVTYDSDATSSACRELAYFFTTGIVVSAFGFPVILARVAVIKWGACGLVLAGNAVIFLTIQGFFLIFGRGDDFSWEQW

Target ORF information:

RefSeq Version	NM_017526.4
Organism	Homo sapiens(human)
Definition	Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017526.4

ORF Insert Sequence:

ATGGCGGGCG TTAAAGCTCT CGTGGCATTA TCCTTCAGTG GGGCTATTGG ACTGACTTTT 
CTTATGCTGG GATGTGCCTT AGAGGATTAT GGCGTTTACT GGCCCTTATT CGTCCTGATT 
TTCCACGCCA TCTCCCCCAT CCCCCATTTC ATTGCCAAAA GAGTCACCTA TGACTCAGAT 
GCAACCAGTA GTGCCTGTCG GGAACTGGCA TATTTCTTCA CTACTGGAAT TGTTGTTTCT 
GCCTTTGGAT TTCCTGTTAT TCTTGCTCGT GTGGCTGTGA TCAAATGGGG AGCCTGCGGC 
CTTGTGTTGG CAGGCAATGC AGTCATTTTC CTTACAATTC AAGGGTTTTT CCTTATATTT 
GGAAGAGGAG ATGATTTTAG CTGGGAGCAG TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LEPROT ( NM_017526.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_059996.1 Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu32203
Clone ID Related Accession (Same CDS sequence)	NM_017526.4 , NM_017526.5
Accession Version	NM_017526.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	leptin receptor gene-related protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from Y12670.1, AC119800.2 and CA434795.1. On Nov 22, 2018 this sequence version replaced NM_017526.4. Transcript Variant: This variant (1) is dominant and encodes the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.208508.1, SRR1803616.221243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000371065.9/ ENSP00000360104.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGGCG TTAAAGCTCT CGTGGCATTA TCCTTCAGTG GGGCTATTGG ACTGACTTTT 
CTTATGCTGG GATGTGCCTT AGAGGATTAT GGCGTTTACT GGCCCTTATT CGTCCTGATT 
TTCCACGCCA TCTCCCCCAT CCCCCATTTC ATTGCCAAAA GAGTCACCTA TGACTCAGAT 
GCAACCAGTA GTGCCTGTCG GGAACTGGCA TATTTCTTCA CTACTGGAAT TGTTGTTTCT 
GCCTTTGGAT TTCCTGTTAT TCTTGCTCGT GTGGCTGTGA TCAAATGGGG AGCCTGCGGC 
CTTGTGTTGG CAGGCAATGC AGTCATTTTC CTTACAATTC AAGGGTTTTT CCTTATATTT 
GGAAGAGGAG ATGATTTTAG CTGGGAGCAG TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059996.1
CDS	58..453
Translation	MAGVKALVALSFSGAIGLTFLMLGCALEDYGVYWPLFVLIFHAISPIPHFIAKRVTYDSDATSSACRELAYFFTTGIVVSAFGFPVILARVAVIKWGACGLVLAGNAVIFLTIQGFFLIFGRGDDFSWEQW

Target ORF information:

RefSeq Version	NM_017526.5
Organism	Homo sapiens(human)
Definition	Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017526.5

ORF Insert Sequence:

ATGGCGGGCG TTAAAGCTCT CGTGGCATTA TCCTTCAGTG GGGCTATTGG ACTGACTTTT 
CTTATGCTGG GATGTGCCTT AGAGGATTAT GGCGTTTACT GGCCCTTATT CGTCCTGATT 
TTCCACGCCA TCTCCCCCAT CCCCCATTTC ATTGCCAAAA GAGTCACCTA TGACTCAGAT 
GCAACCAGTA GTGCCTGTCG GGAACTGGCA TATTTCTTCA CTACTGGAAT TGTTGTTTCT 
GCCTTTGGAT TTCCTGTTAT TCTTGCTCGT GTGGCTGTGA TCAAATGGGG AGCCTGCGGC 
CTTGTGTTGG CAGGCAATGC AGTCATTTTC CTTACAATTC AAGGGTTTTT CCTTATATTT 
GGAAGAGGAG ATGATTTTAG CTGGGAGCAG TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LEPROT ( NM_001198683.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185612.1 Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu05774
Clone ID Related Accession (Same CDS sequence)	NM_001198683.1 , NM_001198683.2
Accession Version	NM_001198683.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	leptin receptor gene-related protein isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC140007.1 and AK074841.1. Transcript Variant: This variant (3) has an alternate 3' sequence, as compared to variant 1. The resulting isoform (3) is much shorter and has a distinct C-terminus, as compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC140007.1, DB509553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

1 61	ATGGCGGGCG TTAAAGCTCT CGTGGCATTA TCCTTCAGTG GGGCTATTGG ACTGACTTTT CTTATGCTGG GATGTGCCTT AGAGGATTAT GGAGATGCCA CAACACCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185612.1
CDS	278..391
Translation	MAGVKALVALSFSGAIGLTFLMLGCALEDYGDATTPS

Target ORF information:

RefSeq Version	NM_001198683.1
Organism	Homo sapiens(human)
Definition	Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198683.1

ORF Insert Sequence:

1 61	ATGGCGGGCG TTAAAGCTCT CGTGGCATTA TCCTTCAGTG GGGCTATTGG ACTGACTTTT CTTATGCTGG GATGTGCCTT AGAGGATTAT GGAGATGCCA CAACACCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LEPROT ( NM_001198683.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185612.1 Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu05774
Clone ID Related Accession (Same CDS sequence)	NM_001198683.1 , NM_001198683.2
Accession Version	NM_001198683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	leptin receptor gene-related protein isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC140007.1 and AK074841.1. On May 31, 2019 this sequence version replaced NM_001198683.1. Transcript Variant: This variant (3) has an alternate 3' sequence, as compared to variant 1. The resulting isoform (3) is much shorter and has a distinct C-terminus, as compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: CB122755.1, BC140007.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

1 61	ATGGCGGGCG TTAAAGCTCT CGTGGCATTA TCCTTCAGTG GGGCTATTGG ACTGACTTTT CTTATGCTGG GATGTGCCTT AGAGGATTAT GGAGATGCCA CAACACCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185612.1
CDS	58..171
Translation	MAGVKALVALSFSGAIGLTFLMLGCALEDYGDATTPS

Target ORF information:

RefSeq Version	NM_001198683.2
Organism	Homo sapiens(human)
Definition	Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198683.2

ORF Insert Sequence:

1 61	ATGGCGGGCG TTAAAGCTCT CGTGGCATTA TCCTTCAGTG GGGCTATTGG ACTGACTTTT CTTATGCTGG GATGTGCCTT AGAGGATTAT GGAGATGCCA CAACACCCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LEPROT ( NM_001198681.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185610.1 Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08389
Clone ID Related Accession (Same CDS sequence)	NM_001198681.1
Accession Version	NM_001198681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Homo sapiens(human)
Product	leptin receptor gene-related protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK309370.1 and AC119800.2. Transcript Variant: This variant (2) has an additional exon in the 5' region, as compared to variant 1. The resulting isoform (2) has a longer and distinct N-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK309370.1, SRR1803611.113482.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820689, SAMN03267751 [ECO:0000348] ##Evidence-Data-END##

ATGCGAGACG GAAAAGGTTT TTTGCAAGGC TTCCTGTATT TTGCTCTCGT GGCATTATCC 
TTCAGTGGGG CTATTGGACT GACTTTTCTT ATGCTGGGAT GTGCCTTAGA GGATTATGGC 
GTTTACTGGC CCTTATTCGT CCTGATTTTC CACGCCATCT CCCCCATCCC CCATTTCATT 
GCCAAAAGAG TCACCTATGA CTCAGATGCA ACCAGTAGTG CCTGTCGGGA ACTGGCATAT 
TTCTTCACTA CTGGAATTGT TGTTTCTGCC TTTGGATTTC CTGTTATTCT TGCTCGTGTG 
GCTGTGATCA AATGGGGAGC CTGCGGCCTT GTGTTGGCAG GCAATGCAGT CATTTTCCTT 
ACAATTCAAG GGTTTTTCCT TATATTTGGA AGAGGAGATG ATTTTAGCTG GGAGCAGTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185610.1
CDS	115..537
Translation	MRDGKGFLQGFLYFALVALSFSGAIGLTFLMLGCALEDYGVYWPLFVLIFHAISPIPHFIAKRVTYDSDATSSACRELAYFFTTGIVVSAFGFPVILARVAVIKWGACGLVLAGNAVIFLTIQGFFLIFGRGDDFSWEQW

Target ORF information:

RefSeq Version	NM_001198681.1
Organism	Homo sapiens(human)
Definition	Homo sapiens leptin receptor overlapping transcript (LEPROT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198681.1

ORF Insert Sequence:

ATGCGAGACG GAAAAGGTTT TTTGCAAGGC TTCCTGTATT TTGCTCTCGT GGCATTATCC 
TTCAGTGGGG CTATTGGACT GACTTTTCTT ATGCTGGGAT GTGCCTTAGA GGATTATGGC 
GTTTACTGGC CCTTATTCGT CCTGATTTTC CACGCCATCT CCCCCATCCC CCATTTCATT 
GCCAAAAGAG TCACCTATGA CTCAGATGCA ACCAGTAGTG CCTGTCGGGA ACTGGCATAT 
TTCTTCACTA CTGGAATTGT TGTTTCTGCC TTTGGATTTC CTGTTATTCT TGCTCGTGTG 
GCTGTGATCA AATGGGGAGC CTGCGGCCTT GTGTTGGCAG GCAATGCAGT CATTTTCCTT 
ACAATTCAAG GGTTTTTCCT TATATTTGGA AGAGGAGATG ATTTTAGCTG GGAGCAGTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

Endospanins regulate a postinternalization step of the leptin receptor endocytic pathway.
The Journal of biological chemistry286(20)17968-81(2011 May)
S?ron K,Couturier C,Belouzard S,Bacart J,Mont? D,Corset L,Bocquet O,Dam J,Vauthier V,Lec?ur C,Bailleul B,Hoflack B,Froguel P,Jockers R,Rouill? Y

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,

Copy number variation at leptin receptor gene locus associated with metabolic traits and the risk of type 2 diabetes mellitus.
BMC genomics11426(2010 Jul)
Jeon JP,Shim SM,Nam HY,Ryu GM,Hong EJ,Kim HL,Han BG

Identification of type 2 diabetes-associated combination of SNPs using support vector machine.
BMC genetics1126(2010 Apr)
Ban HJ,Heo JY,Oh KS,Park KJ