PEX1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PEX1
Entrez Gene ID	5189
Full Name	peroxisomal biogenesis factor 1
Synonyms	HMLR1,PBD1A,PBD1B,ZWS,ZWS1
General protein information	Preferred Names peroxisomal biogenesis factor 1 Names peroxisome biogenesis factor 1 Zellweger syndrome peroxin-1 peroxisome biogenesis disorder protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q21.2
Summary	This gene encodes a member of the AAA ATPase family, a large group of ATPases associated with diverse cellular activities. This protein is cytoplasmic but is often anchored to a peroxisomal membrane where it forms a heteromeric complex and plays a role in the import of proteins into peroxisomes and peroxisome biogenesis. Mutations in this gene have been associated with complementation group 1 peroxisomal disorders such as neonatal adrenoleukodystrophy, infantile Refsum disease, and Zellweger syndrome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013].
Disorder MIM:	602136
Disorder Html:	Peroxisome biogenesis disorder 1A (Zellweger), 214100 (3); Peroxisome biogenesis disorder 1B (NALD/IRD), 601539 (3); Heimler syndrome 1, 234580 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000466.2	NP_000457.1	peroxisome biogenesis factor 1 isoform 1
NM_001282677.1	NP_001269606.1	peroxisome biogenesis factor 1 isoform 2
NM_001282677.2	NP_001269606.1	peroxisome biogenesis factor 1 isoform 2
NM_001282678.1	NP_001269607.1	peroxisome biogenesis factor 1 isoform 3
NM_000466.3	NP_000457.1	peroxisome biogenesis factor 1 isoform 1
NM_001282678.2	NP_001269607.1	peroxisome biogenesis factor 1 isoform 3
XM_017012319.1	XP_016867808.1	peroxisome biogenesis factor 1 isoform X1
XM_017012319.2	XP_016867808.1	peroxisome biogenesis factor 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PEX1	XP_532459.1
Pan troglodytes (chimpanzee)	PEX1	XP_519198.2
Gallus gallus (chicken)	PEX1	XP_004939352.1
Xenopus tropicalis (tropical clawed frog)	pex1	XP_004915478.1
Homo sapiens (human)	PEX1	NP_000457.1
Mus musculus (house mouse)	Pex1	NP_082053.1
Danio rerio (zebrafish)	pex1	XP_003200668.1
	PEX1	XP_001101055.2
Bos taurus (cattle)	PEX1	NP_001179000.1
Rattus norvegicus (Norway rat)	Pex1	NP_001102690.1

Gene Ontology
Bibliography

Related articles in PubMed

Exome sequencing reveals novel genetic loci influencing obesity-related traits in Hispanic children.
Sabo A, Mishra P, Dugan-Perez S, Voruganti VS, Kent JW Jr, Kalra D, Cole SA, Comuzzie AG, Muzny DM, Gibbs RA, Butte NF
Obesity (Silver Spring, Md.)25(7)1270-1276(2017 Jul)

Novel compound heterozygous mutations in the PEX1 gene in two Chinese newborns with Zellweger syndrome based on whole exome sequencing.
Ge MM, Hu L, Li Z, Cheng G, Yan K, Kong Y, Wang H, Yang L, Zhou W
Clinica chimica acta; international journal of clinical chemistry47024-28(2017 Jul)

Spectrum of PEX1 and PEX6 variants in Heimler syndrome.
Smith CE, Poulter JA, Levin AV, Capasso JE, Price S, Ben-Yosef T, Sharony R, Newman WG, Shore RC, Brookes SJ, Mighell AJ, Inglehearn CF
European journal of human genetics : EJHG24(11)1565-1571(2016 11)

Severe early onset retinitis pigmentosa in a Moroccan patient with Heimler syndrome due to novel homozygous mutation of PEX1 gene.
Ratbi I, Jaouad IC, Elorch H, Al-Sheqaih N, Elalloussi M, Lyahyai J, Berraho A, Newman WG, Sefiani A
European journal of medical genetics59(10)507-11(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Major finding is linking peroxisome biogenesis factor 1 (PEX1) to obesity phenotypes, a novel mechanism of peroxisomal biogenesis and metabolism underlying the development of childhood obesity.
Title: Exome sequencing reveals novel genetic loci influencing obesity-related traits in Hispanic children.

heterozygous mutations in the PEX1 gene in two Chinese newborns with Zellweger syndrome
Title: Novel compound heterozygous mutations in the PEX1 gene in two Chinese newborns with Zellweger syndrome based on whole exome sequencing.

A combination of a known missense and novel frameshift variant in PEX1 identified in a family with Heimler syndrome.
Title: Spectrum of PEX1 and PEX6 variants in Heimler syndrome.

As standard biochemical screening of blood for evidence of a peroxisomal disorder did not provide a diagnosis in the individuals with Heimler syndrome, patients with sensorineural hearing lossand retinal pigmentation should have mutation analysis of PEX1 and PEX6 genes.
Title: Severe early onset retinitis pigmentosa in a Moroccan patient with Heimler syndrome due to novel homozygous mutation of PEX1 gene.

Our structural data suggest that the tilting of a central segment of a Pex1-Pex6 pair is responsible for polypeptide movement.
Title: Structures of the double-ring AAA ATPase Pex1-Pex6 involved in peroxisome biogenesis.

The following PEX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PEX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25738	NM_000466.3 Latest version!	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu41614	XM_017012319.2 Latest version!	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu16877	NM_001282678.2 Latest version!	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu27457	NM_001282677.2 Latest version!	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu27457	NM_001282677.1	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu25738	NM_000466.2	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu16877	NM_001282678.1	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu41614	XM_017012319.1	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PEX1 ( NM_000466.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000457.1 Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25738
Clone ID Related Accession (Same CDS sequence)	NM_000466.2 , NM_000466.3
Accession Version	NM_000466.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	peroxisome biogenesis factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA313282.1, BC035575.1, DB461678.1, AF026086.1 and AI377121.1. This sequence is a reference standard in the RefSeqGene project. On Apr 28, 2006 this sequence version replaced NM_000466.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.204223.1, AF026086.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGGCA GCGATCGCCT GGCGGGTGCT GGGGGAGGCG GGGCGGCAGT GACTGTGGCC 
TTCACCAACG CTCGCGACTG CTTCCTCCAC CTGCCGCGGC GTCTCGTGGC CCAGCTGCAT 
CTGCTGCAGA ATCAAGCTAT AGAAGTGGTC TGGAGTCACC AGCCTGCATT CTTGAGCTGG 
GTGGAAGGCA GGCATTTTAG TGATCAAGGT GAAAATGTGG CTGAAATTAA CAGACAAGTT 
GGTCAAAAAC TTGGACTCTC AAATGGGGGA CAGGTATTTC TCAAGCCATG TTCCCATGTG 
GTATCTTGTC AACAAGTTGA GGTGGAACCC CTCTCAGCAG ATGATTGGGA GATACTGGAG 
CTGCATGCTG TTTCCCTTGA ACAACATCTT CTAGATCAAA TTCGAATAGT TTTTCCAAAA 
GCCATTTTTC CTGTTTGGGT TGATCAACAA ACGTACATAT TTATCCAAAT TGTTGCACTA 
ATACCAGCTG CCTCTTATGG AAGGCTGGAA ACTGACACCA AACTCCTTAT TCAGCCAAAG 
ACACGCCGAG CCAAAGAGAA TACATTTTCA AAAGCTGATG CTGAATATAA AAAACTTCAT 
AGTTATGGAA GAGACCAGAA AGGAATGATG AAAGAACTTC AAACCAAGCA ACTTCAGTCA 
AATACTGTGG GAATCACTGA ATCTAATGAA AACGAGTCAG AGATTCCAGT TGACTCATCA 
TCAGTAGCAA GTTTATGGAC TATGATAGGA AGCATTTTTT CCTTTCAATC TGAGAAGAAA 
CAAGAGACAT CTTGGGGTTT AACTGAAATC AATGCATTCA AAAATATGCA GTCAAAGGTT 
GTTCCTCTAG ACAATATTTT CAGAGTATGC AAATCTCAAC CTCCTAGTAT ATATAACGCG 
TCAGCAACCT CTGTTTTTCA TAAACACTGT GCCATTCATG TATTTCCATG GGACCAGGAA 
TATTTTGATG TAGAGCCCAG CTTTACTGTG ACATATGGAA AGCTAGTTAA GCTACTTTCT 
CCAAAGCAAC AGCAAAGTAA AACAAAACAA AATGTGTTAT CACCTGAAAA AGAGAAGCAG 
ATGTCAGAGC CACTAGATCA AAAAAAAATT AGGTCAGATC ATAATGAAGA AGATGAGAAG 
GCCTGTGTGC TACAAGTAGT CTGGAATGGA CTTGAAGAAT TGAACAATGC CATCAAATAT 
ACCAAAAATG TAGAAGTTCT CCATCTTGGG AAAGTCTGGA TTCCAGATGA CCTGAGGAAG 
AGACTAAATA TAGAAATGCA TGCCGTAGTC AGGATAACTC CAGTGGAAGT TACCCCTAAA 
ATTCCAAGAT CTCTAAAGTT ACAACCTAGA GAGAATTTAC CTAAAGACAT AAGTGAAGAA 
GACATAAAAA CTGTATTTTA TTCATGGCTA CAGCAGTCTA CTACCACCAT GCTTCCTTTG 
GTAATATCAG AGGAAGAATT TATTAAGCTG GAAACTAAAG ATGGACTGAA GGAATTTTCT 
CTGAGTATAG TTCATTCTTG GGAAAAAGAA AAAGATAAAA ATATTTTTCT GTTGAGTCCC 
AATTTGCTGC AGAAGACTAC AATACAAGTC CTTCTAGATC CTATGGTAAA AGAAGAAAAC 
AGTGAGGAAA TTGACTTTAT TCTTCCTTTT TTAAAGCTGA GCTCTTTGGG AGGAGTGAAT 
TCCTTAGGCG TATCCTCCTT GGAGCACATC ACTCACAGCC TCCTGGGACG CCCTTTGTCT 
CGGCAGCTGA TGTCTCTTGT TGCAGGACTT AGGAATGGAG CTCTTTTACT CACAGGAGGA 
AAGGGAAGTG GAAAATCAAC TTTAGCCAAA GCAATCTGTA AAGAAGCATT TGACAAACTG 
GATGCCCATG TGGAGAGAGT TGACTGTAAA GCTTTACGAG GAAAAAGGCT TGAAAACATA 
CAAAAAACCC TAGAGGTGGC TTTCTCAGAG GCAGTGTGGA TGCAGCCATC TGTTGTCCTG 
CTGGATGACC TTGACCTCAT TGCTGGACTG CCTGCTGTCC CGGAACATGA GCACAGTCCT 
GATGCGGTGC AGAGCCAGCG GCTTGCTCAT GCTTTGAATG ATATGATAAA AGAGTTTATC 
TCCATGGGAA GTTTGGTTGC ACTGATTGCC ACAAGTCAGT CTCAGCAATC TCTACATCCT 
TTACTTGTTT CTGCTCAAGG AGTTCACATA TTTCAGTGCG TCCAACACAT TCAGCCTCCT 
AATCAGGAAC AAAGATGTGA AATTCTGTGT AATGTAATAA AAAATAAATT GGACTGTGAT 
ATAAACAAGT TCACCGATCT TGACCTGCAG CATGTAGCTA AAGAAACTGG CGGGTTTGTG 
GCTAGAGATT TTACAGTACT TGTGGATCGA GCCATACATT CTCGACTCTC TCGTCAGAGT 
ATATCCACCA GAGAAAAATT AGTTTTAACA ACATTGGACT TCCAAAAGGC TCTCCGCGGA 
TTTCTTCCTG CGTCTTTGCG AAGTGTCAAC CTGCATAAAC CTAGAGACCT GGGTTGGGAC 
AAGATTGGTG GGTTACATGA AGTTAGGCAG ATACTCATGG ATACTATCCA GTTACCTGCC 
AAGTATCCAG AATTATTTGC AAACTTGCCC ATACGACAAA GAACAGGAAT ACTGTTGTAT 
GGTCCGCCTG GAACAGGAAA AACCTTACTA GCTGGGGTAA TTGCACGAGA GAGTAGAATG 
AATTTTATAA GTGTCAAGGG GCCAGAGTTA CTCAGCAAAT ACATTGGAGC AAGTGAACAA 
GCTGTTCGGG ATATTTTTAT TAGAGCACAG GCTGCAAAGC CCTGCATTCT TTTCTTTGAT 
GAATTTGAAT CCATTGCTCC TCGGCGGGGT CATGATAATA CAGGAGTTAC AGACCGAGTA 
GTTAACCAGT TGCTGACTCA GTTGGATGGA GTAGAAGGCT TACAGGGTGT TTATGTATTG 
GCTGCTACTA GTCGCCCTGA CTTGATTGAC CCTGCCCTGC TTAGGCCTGG TCGACTAGAT 
AAATGTGTAT ACTGTCCTCC TCCTGATCAG GTGTCACGTC TTGAAATTTT AAATGTCCTC 
AGTGACTCTC TACCTCTGGC AGATGATGTT GACCTTCAGC ATGTAGCATC AGTAACTGAC 
TCCTTTACTG GAGCTGATCT GAAAGCTTTA CTTTACAATG CCCAATTGGA GGCCTTACAT 
GGAATGCTGC TCTCGAGTGG ACTCCAGGAT GGAAGTTCCA GCTCTGATAG TGACCTAAGT 
CTGTCTTCAA TGGTCTTTCT TAACCATAGC AGTGGCTCTG ACGATTCAGC TGGAGATGGA 
GAATGTGGCT TAGATCAGTC CCTTGTTTCT TTAGAGATGT CCGAGATCCT TCCAGATGAA 
TCAAAATTCA ATATGTACCG GCTCTACTTT GGAAGCTCTT ATGAATCAGA ACTTGGAAAT 
GGAACCTCTT CTGATTTGAG CTCACAATGT CTCTCTGCAC CAAGCTCCAT GACTCAGGAT 
TTGCCTGGAG TTCCTGGGAA AGACCAGTTG TTTTCACAGC CTCCAGTGTT AAGGACAGCT 
TCACAAGAGG GTTGCCAAGA ACTTACACAA GAACAAAGAG ATCAACTGAG GGCAGATATC 
AGTATTATCA AAGGCAGATA CCGGAGCCAA AGTGGAGAGG ACGAATCCAT GAACCAACCA 
GGACCAATCA AAACCAGACT GGCTATTAGT CAGTCACATT TAATGACTGC ACTTGGTCAC 
ACAAGACCAT CCATTAGTGA AGATGACTGG AAGAATTTTG CTGAGCTATA TGAAAGCTTT 
CAAAATCCAA AGAGGAGAAA AAATCAAAGT GGAACAATGT TTCGACCTGG ACAGAAAGTA 
ACTTTAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000457.1
CDS	97..3948
Translation	MWGSDRLAGAGGGGAAVTVAFTNARDCFLHLPRRLVAQLHLLQNQAIEVVWSHQPAFLSWVEGRHFSDQGENVAEINRQVGQKLGLSNGGQVFLKPCSHVVSCQQVEVEPLSADDWEILELHAVSLEQHLLDQIRIVFPKAIFPVWVDQQTYIFIQIVALIPAASYGRLETDTKLLIQPKTRRAKENTFSKADAEYKKLHSYGRDQKGMMKELQTKQLQSNTVGITESNENESEIPVDSSSVASLWTMIGSIFSFQSEKKQETSWGLTEINAFKNMQSKVVPLDNIFRVCKSQPPSIYNASATSVFHKHCAIHVFPWDQEYFDVEPSFTVTYGKLVKLLSPKQQQSKTKQNVLSPEKEKQMSEPLDQKKIRSDHNEEDEKACVLQVVWNGLEELNNAIKYTKNVEVLHLGKVWIPDDLRKRLNIEMHAVVRITPVEVTPKIPRSLKLQPRENLPKDISEEDIKTVFYSWLQQSTTTMLPLVISEEEFIKLETKDGLKEFSLSIVHSWEKEKDKNIFLLSPNLLQKTTIQVLLDPMVKEENSEEIDFILPFLKLSSLGGVNSLGVSSLEHITHSLLGRPLSRQLMSLVAGLRNGALLLTGGKGSGKSTLAKAICKEAFDKLDAHVERVDCKALRGKRLENIQKTLEVAFSEAVWMQPSVVLLDDLDLIAGLPAVPEHEHSPDAVQSQRLAHALNDMIKEFISMGSLVALIATSQSQQSLHPLLVSAQGVHIFQCVQHIQPPNQEQRCEILCNVIKNKLDCDINKFTDLDLQHVAKETGGFVARDFTVLVDRAIHSRLSRQSISTREKLVLTTLDFQKALRGFLPASLRSVNLHKPRDLGWDKIGGLHEVRQILMDTIQLPAKYPELFANLPIRQRTGILLYGPPGTGKTLLAGVIARESRMNFISVKGPELLSKYIGASEQAVRDIFIRAQAAKPCILFFDEFESIAPRRGHDNTGVTDRVVNQLLTQLDGVEGLQGVYVLAATSRPDLIDPALLRPGRLDKCVYCPPPDQVSRLEILNVLSDSLPLADDVDLQHVASVTDSFTGADLKALLYNAQLEALHGMLLSSGLQDGSSSSDSDLSLSSMVFLNHSSGSDDSAGDGECGLDQSLVSLEMSEILPDESKFNMYRLYFGSSYESELGNGTSSDLSSQCLSAPSSMTQDLPGVPGKDQLFSQPPVLRTASQEGCQELTQEQRDQLRADISIIKGRYRSQSGEDESMNQPGPIKTRLAISQSHLMTALGHTRPSISEDDWKNFAELYESFQNPKRRKNQSGTMFRPGQKVTLA

Target ORF information:

RefSeq Version	NM_000466.2
Organism	Homo sapiens(human)
Definition	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000466.2

ORF Insert Sequence:

ATGTGGGGCA GCGATCGCCT GGCGGGTGCT GGGGGAGGCG GGGCGGCAGT GACTGTGGCC 
TTCACCAACG CTCGCGACTG CTTCCTCCAC CTGCCGCGGC GTCTCGTGGC CCAGCTGCAT 
CTGCTGCAGA ATCAAGCTAT AGAAGTGGTC TGGAGTCACC AGCCTGCATT CTTGAGCTGG 
GTGGAAGGCA GGCATTTTAG TGATCAAGGT GAAAATGTGG CTGAAATTAA CAGACAAGTT 
GGTCAAAAAC TTGGACTCTC AAATGGGGGA CAGGTATTTC TCAAGCCATG TTCCCATGTG 
GTATCTTGTC AACAAGTTGA GGTGGAACCC CTCTCAGCAG ATGATTGGGA GATACTGGAG 
CTGCATGCTG TTTCCCTTGA ACAACATCTT CTAGATCAAA TTCGAATAGT TTTTCCAAAA 
GCCATTTTTC CTGTTTGGGT TGATCAACAA ACGTACATAT TTATCCAAAT TGTTGCACTA 
ATACCAGCTG CCTCTTATGG AAGGCTGGAA ACTGACACCA AACTCCTTAT TCAGCCAAAG 
ACACGCCGAG CCAAAGAGAA TACATTTTCA AAAGCTGATG CTGAATATAA AAAACTTCAT 
AGTTATGGAA GAGACCAGAA AGGAATGATG AAAGAACTTC AAACCAAGCA ACTTCAGTCA 
AATACTGTGG GAATCACTGA ATCTAATGAA AACGAGTCAG AGATTCCAGT TGACTCATCA 
TCAGTAGCAA GTTTATGGAC TATGATAGGA AGCATTTTTT CCTTTCAATC TGAGAAGAAA 
CAAGAGACAT CTTGGGGTTT AACTGAAATC AATGCATTCA AAAATATGCA GTCAAAGGTT 
GTTCCTCTAG ACAATATTTT CAGAGTATGC AAATCTCAAC CTCCTAGTAT ATATAACGCG 
TCAGCAACCT CTGTTTTTCA TAAACACTGT GCCATTCATG TATTTCCATG GGACCAGGAA 
TATTTTGATG TAGAGCCCAG CTTTACTGTG ACATATGGAA AGCTAGTTAA GCTACTTTCT 
CCAAAGCAAC AGCAAAGTAA AACAAAACAA AATGTGTTAT CACCTGAAAA AGAGAAGCAG 
ATGTCAGAGC CACTAGATCA AAAAAAAATT AGGTCAGATC ATAATGAAGA AGATGAGAAG 
GCCTGTGTGC TACAAGTAGT CTGGAATGGA CTTGAAGAAT TGAACAATGC CATCAAATAT 
ACCAAAAATG TAGAAGTTCT CCATCTTGGG AAAGTCTGGA TTCCAGATGA CCTGAGGAAG 
AGACTAAATA TAGAAATGCA TGCCGTAGTC AGGATAACTC CAGTGGAAGT TACCCCTAAA 
ATTCCAAGAT CTCTAAAGTT ACAACCTAGA GAGAATTTAC CTAAAGACAT AAGTGAAGAA 
GACATAAAAA CTGTATTTTA TTCATGGCTA CAGCAGTCTA CTACCACCAT GCTTCCTTTG 
GTAATATCAG AGGAAGAATT TATTAAGCTG GAAACTAAAG ATGGACTGAA GGAATTTTCT 
CTGAGTATAG TTCATTCTTG GGAAAAAGAA AAAGATAAAA ATATTTTTCT GTTGAGTCCC 
AATTTGCTGC AGAAGACTAC AATACAAGTC CTTCTAGATC CTATGGTAAA AGAAGAAAAC 
AGTGAGGAAA TTGACTTTAT TCTTCCTTTT TTAAAGCTGA GCTCTTTGGG AGGAGTGAAT 
TCCTTAGGCG TATCCTCCTT GGAGCACATC ACTCACAGCC TCCTGGGACG CCCTTTGTCT 
CGGCAGCTGA TGTCTCTTGT TGCAGGACTT AGGAATGGAG CTCTTTTACT CACAGGAGGA 
AAGGGAAGTG GAAAATCAAC TTTAGCCAAA GCAATCTGTA AAGAAGCATT TGACAAACTG 
GATGCCCATG TGGAGAGAGT TGACTGTAAA GCTTTACGAG GAAAAAGGCT TGAAAACATA 
CAAAAAACCC TAGAGGTGGC TTTCTCAGAG GCAGTGTGGA TGCAGCCATC TGTTGTCCTG 
CTGGATGACC TTGACCTCAT TGCTGGACTG CCTGCTGTCC CGGAACATGA GCACAGTCCT 
GATGCGGTGC AGAGCCAGCG GCTTGCTCAT GCTTTGAATG ATATGATAAA AGAGTTTATC 
TCCATGGGAA GTTTGGTTGC ACTGATTGCC ACAAGTCAGT CTCAGCAATC TCTACATCCT 
TTACTTGTTT CTGCTCAAGG AGTTCACATA TTTCAGTGCG TCCAACACAT TCAGCCTCCT 
AATCAGGAAC AAAGATGTGA AATTCTGTGT AATGTAATAA AAAATAAATT GGACTGTGAT 
ATAAACAAGT TCACCGATCT TGACCTGCAG CATGTAGCTA AAGAAACTGG CGGGTTTGTG 
GCTAGAGATT TTACAGTACT TGTGGATCGA GCCATACATT CTCGACTCTC TCGTCAGAGT 
ATATCCACCA GAGAAAAATT AGTTTTAACA ACATTGGACT TCCAAAAGGC TCTCCGCGGA 
TTTCTTCCTG CGTCTTTGCG AAGTGTCAAC CTGCATAAAC CTAGAGACCT GGGTTGGGAC 
AAGATTGGTG GGTTACATGA AGTTAGGCAG ATACTCATGG ATACTATCCA GTTACCTGCC 
AAGTATCCAG AATTATTTGC AAACTTGCCC ATACGACAAA GAACAGGAAT ACTGTTGTAT 
GGTCCGCCTG GAACAGGAAA AACCTTACTA GCTGGGGTAA TTGCACGAGA GAGTAGAATG 
AATTTTATAA GTGTCAAGGG GCCAGAGTTA CTCAGCAAAT ACATTGGAGC AAGTGAACAA 
GCTGTTCGGG ATATTTTTAT TAGAGCACAG GCTGCAAAGC CCTGCATTCT TTTCTTTGAT 
GAATTTGAAT CCATTGCTCC TCGGCGGGGT CATGATAATA CAGGAGTTAC AGACCGAGTA 
GTTAACCAGT TGCTGACTCA GTTGGATGGA GTAGAAGGCT TACAGGGTGT TTATGTATTG 
GCTGCTACTA GTCGCCCTGA CTTGATTGAC CCTGCCCTGC TTAGGCCTGG TCGACTAGAT 
AAATGTGTAT ACTGTCCTCC TCCTGATCAG GTGTCACGTC TTGAAATTTT AAATGTCCTC 
AGTGACTCTC TACCTCTGGC AGATGATGTT GACCTTCAGC ATGTAGCATC AGTAACTGAC 
TCCTTTACTG GAGCTGATCT GAAAGCTTTA CTTTACAATG CCCAATTGGA GGCCTTACAT 
GGAATGCTGC TCTCGAGTGG ACTCCAGGAT GGAAGTTCCA GCTCTGATAG TGACCTAAGT 
CTGTCTTCAA TGGTCTTTCT TAACCATAGC AGTGGCTCTG ACGATTCAGC TGGAGATGGA 
GAATGTGGCT TAGATCAGTC CCTTGTTTCT TTAGAGATGT CCGAGATCCT TCCAGATGAA 
TCAAAATTCA ATATGTACCG GCTCTACTTT GGAAGCTCTT ATGAATCAGA ACTTGGAAAT 
GGAACCTCTT CTGATTTGAG CTCACAATGT CTCTCTGCAC CAAGCTCCAT GACTCAGGAT 
TTGCCTGGAG TTCCTGGGAA AGACCAGTTG TTTTCACAGC CTCCAGTGTT AAGGACAGCT 
TCACAAGAGG GTTGCCAAGA ACTTACACAA GAACAAAGAG ATCAACTGAG GGCAGATATC 
AGTATTATCA AAGGCAGATA CCGGAGCCAA AGTGGAGAGG ACGAATCCAT GAACCAACCA 
GGACCAATCA AAACCAGACT GGCTATTAGT CAGTCACATT TAATGACTGC ACTTGGTCAC 
ACAAGACCAT CCATTAGTGA AGATGACTGG AAGAATTTTG CTGAGCTATA TGAAAGCTTT 
CAAAATCCAA AGAGGAGAAA AAATCAAAGT GGAACAATGT TTCGACCTGG ACAGAAAGTA 
ACTTTAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEX1 ( NM_000466.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000457.1 Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25738
Clone ID Related Accession (Same CDS sequence)	NM_000466.2 , NM_000466.3
Accession Version	NM_000466.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	peroxisome biogenesis factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA313282.1, BC035575.1, DB461678.1, AF026086.1 and AI377121.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000466.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035575.1, SRR1660805.204223.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000248633.9/ ENSP00000248633.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGGGCA GCGATCGCCT GGCGGGTGCT GGGGGAGGCG GGGCGGCAGT GACTGTGGCC 
TTCACCAACG CTCGCGACTG CTTCCTCCAC CTGCCGCGGC GTCTCGTGGC CCAGCTGCAT 
CTGCTGCAGA ATCAAGCTAT AGAAGTGGTC TGGAGTCACC AGCCTGCATT CTTGAGCTGG 
GTGGAAGGCA GGCATTTTAG TGATCAAGGT GAAAATGTGG CTGAAATTAA CAGACAAGTT 
GGTCAAAAAC TTGGACTCTC AAATGGGGGA CAGGTATTTC TCAAGCCATG TTCCCATGTG 
GTATCTTGTC AACAAGTTGA GGTGGAACCC CTCTCAGCAG ATGATTGGGA GATACTGGAG 
CTGCATGCTG TTTCCCTTGA ACAACATCTT CTAGATCAAA TTCGAATAGT TTTTCCAAAA 
GCCATTTTTC CTGTTTGGGT TGATCAACAA ACGTACATAT TTATCCAAAT TGTTGCACTA 
ATACCAGCTG CCTCTTATGG AAGGCTGGAA ACTGACACCA AACTCCTTAT TCAGCCAAAG 
ACACGCCGAG CCAAAGAGAA TACATTTTCA AAAGCTGATG CTGAATATAA AAAACTTCAT 
AGTTATGGAA GAGACCAGAA AGGAATGATG AAAGAACTTC AAACCAAGCA ACTTCAGTCA 
AATACTGTGG GAATCACTGA ATCTAATGAA AACGAGTCAG AGATTCCAGT TGACTCATCA 
TCAGTAGCAA GTTTATGGAC TATGATAGGA AGCATTTTTT CCTTTCAATC TGAGAAGAAA 
CAAGAGACAT CTTGGGGTTT AACTGAAATC AATGCATTCA AAAATATGCA GTCAAAGGTT 
GTTCCTCTAG ACAATATTTT CAGAGTATGC AAATCTCAAC CTCCTAGTAT ATATAACGCG 
TCAGCAACCT CTGTTTTTCA TAAACACTGT GCCATTCATG TATTTCCATG GGACCAGGAA 
TATTTTGATG TAGAGCCCAG CTTTACTGTG ACATATGGAA AGCTAGTTAA GCTACTTTCT 
CCAAAGCAAC AGCAAAGTAA AACAAAACAA AATGTGTTAT CACCTGAAAA AGAGAAGCAG 
ATGTCAGAGC CACTAGATCA AAAAAAAATT AGGTCAGATC ATAATGAAGA AGATGAGAAG 
GCCTGTGTGC TACAAGTAGT CTGGAATGGA CTTGAAGAAT TGAACAATGC CATCAAATAT 
ACCAAAAATG TAGAAGTTCT CCATCTTGGG AAAGTCTGGA TTCCAGATGA CCTGAGGAAG 
AGACTAAATA TAGAAATGCA TGCCGTAGTC AGGATAACTC CAGTGGAAGT TACCCCTAAA 
ATTCCAAGAT CTCTAAAGTT ACAACCTAGA GAGAATTTAC CTAAAGACAT AAGTGAAGAA 
GACATAAAAA CTGTATTTTA TTCATGGCTA CAGCAGTCTA CTACCACCAT GCTTCCTTTG 
GTAATATCAG AGGAAGAATT TATTAAGCTG GAAACTAAAG ATGGACTGAA GGAATTTTCT 
CTGAGTATAG TTCATTCTTG GGAAAAAGAA AAAGATAAAA ATATTTTTCT GTTGAGTCCC 
AATTTGCTGC AGAAGACTAC AATACAAGTC CTTCTAGATC CTATGGTAAA AGAAGAAAAC 
AGTGAGGAAA TTGACTTTAT TCTTCCTTTT TTAAAGCTGA GCTCTTTGGG AGGAGTGAAT 
TCCTTAGGCG TATCCTCCTT GGAGCACATC ACTCACAGCC TCCTGGGACG CCCTTTGTCT 
CGGCAGCTGA TGTCTCTTGT TGCAGGACTT AGGAATGGAG CTCTTTTACT CACAGGAGGA 
AAGGGAAGTG GAAAATCAAC TTTAGCCAAA GCAATCTGTA AAGAAGCATT TGACAAACTG 
GATGCCCATG TGGAGAGAGT TGACTGTAAA GCTTTACGAG GAAAAAGGCT TGAAAACATA 
CAAAAAACCC TAGAGGTGGC TTTCTCAGAG GCAGTGTGGA TGCAGCCATC TGTTGTCCTG 
CTGGATGACC TTGACCTCAT TGCTGGACTG CCTGCTGTCC CGGAACATGA GCACAGTCCT 
GATGCGGTGC AGAGCCAGCG GCTTGCTCAT GCTTTGAATG ATATGATAAA AGAGTTTATC 
TCCATGGGAA GTTTGGTTGC ACTGATTGCC ACAAGTCAGT CTCAGCAATC TCTACATCCT 
TTACTTGTTT CTGCTCAAGG AGTTCACATA TTTCAGTGCG TCCAACACAT TCAGCCTCCT 
AATCAGGAAC AAAGATGTGA AATTCTGTGT AATGTAATAA AAAATAAATT GGACTGTGAT 
ATAAACAAGT TCACCGATCT TGACCTGCAG CATGTAGCTA AAGAAACTGG CGGGTTTGTG 
GCTAGAGATT TTACAGTACT TGTGGATCGA GCCATACATT CTCGACTCTC TCGTCAGAGT 
ATATCCACCA GAGAAAAATT AGTTTTAACA ACATTGGACT TCCAAAAGGC TCTCCGCGGA 
TTTCTTCCTG CGTCTTTGCG AAGTGTCAAC CTGCATAAAC CTAGAGACCT GGGTTGGGAC 
AAGATTGGTG GGTTACATGA AGTTAGGCAG ATACTCATGG ATACTATCCA GTTACCTGCC 
AAGTATCCAG AATTATTTGC AAACTTGCCC ATACGACAAA GAACAGGAAT ACTGTTGTAT 
GGTCCGCCTG GAACAGGAAA AACCTTACTA GCTGGGGTAA TTGCACGAGA GAGTAGAATG 
AATTTTATAA GTGTCAAGGG GCCAGAGTTA CTCAGCAAAT ACATTGGAGC AAGTGAACAA 
GCTGTTCGGG ATATTTTTAT TAGAGCACAG GCTGCAAAGC CCTGCATTCT TTTCTTTGAT 
GAATTTGAAT CCATTGCTCC TCGGCGGGGT CATGATAATA CAGGAGTTAC AGACCGAGTA 
GTTAACCAGT TGCTGACTCA GTTGGATGGA GTAGAAGGCT TACAGGGTGT TTATGTATTG 
GCTGCTACTA GTCGCCCTGA CTTGATTGAC CCTGCCCTGC TTAGGCCTGG TCGACTAGAT 
AAATGTGTAT ACTGTCCTCC TCCTGATCAG GTGTCACGTC TTGAAATTTT AAATGTCCTC 
AGTGACTCTC TACCTCTGGC AGATGATGTT GACCTTCAGC ATGTAGCATC AGTAACTGAC 
TCCTTTACTG GAGCTGATCT GAAAGCTTTA CTTTACAATG CCCAATTGGA GGCCTTACAT 
GGAATGCTGC TCTCGAGTGG ACTCCAGGAT GGAAGTTCCA GCTCTGATAG TGACCTAAGT 
CTGTCTTCAA TGGTCTTTCT TAACCATAGC AGTGGCTCTG ACGATTCAGC TGGAGATGGA 
GAATGTGGCT TAGATCAGTC CCTTGTTTCT TTAGAGATGT CCGAGATCCT TCCAGATGAA 
TCAAAATTCA ATATGTACCG GCTCTACTTT GGAAGCTCTT ATGAATCAGA ACTTGGAAAT 
GGAACCTCTT CTGATTTGAG CTCACAATGT CTCTCTGCAC CAAGCTCCAT GACTCAGGAT 
TTGCCTGGAG TTCCTGGGAA AGACCAGTTG TTTTCACAGC CTCCAGTGTT AAGGACAGCT 
TCACAAGAGG GTTGCCAAGA ACTTACACAA GAACAAAGAG ATCAACTGAG GGCAGATATC 
AGTATTATCA AAGGCAGATA CCGGAGCCAA AGTGGAGAGG ACGAATCCAT GAACCAACCA 
GGACCAATCA AAACCAGACT GGCTATTAGT CAGTCACATT TAATGACTGC ACTTGGTCAC 
ACAAGACCAT CCATTAGTGA AGATGACTGG AAGAATTTTG CTGAGCTATA TGAAAGCTTT 
CAAAATCCAA AGAGGAGAAA AAATCAAAGT GGAACAATGT TTCGACCTGG ACAGAAAGTA 
ACTTTAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000457.1
CDS	86..3937
Translation	MWGSDRLAGAGGGGAAVTVAFTNARDCFLHLPRRLVAQLHLLQNQAIEVVWSHQPAFLSWVEGRHFSDQGENVAEINRQVGQKLGLSNGGQVFLKPCSHVVSCQQVEVEPLSADDWEILELHAVSLEQHLLDQIRIVFPKAIFPVWVDQQTYIFIQIVALIPAASYGRLETDTKLLIQPKTRRAKENTFSKADAEYKKLHSYGRDQKGMMKELQTKQLQSNTVGITESNENESEIPVDSSSVASLWTMIGSIFSFQSEKKQETSWGLTEINAFKNMQSKVVPLDNIFRVCKSQPPSIYNASATSVFHKHCAIHVFPWDQEYFDVEPSFTVTYGKLVKLLSPKQQQSKTKQNVLSPEKEKQMSEPLDQKKIRSDHNEEDEKACVLQVVWNGLEELNNAIKYTKNVEVLHLGKVWIPDDLRKRLNIEMHAVVRITPVEVTPKIPRSLKLQPRENLPKDISEEDIKTVFYSWLQQSTTTMLPLVISEEEFIKLETKDGLKEFSLSIVHSWEKEKDKNIFLLSPNLLQKTTIQVLLDPMVKEENSEEIDFILPFLKLSSLGGVNSLGVSSLEHITHSLLGRPLSRQLMSLVAGLRNGALLLTGGKGSGKSTLAKAICKEAFDKLDAHVERVDCKALRGKRLENIQKTLEVAFSEAVWMQPSVVLLDDLDLIAGLPAVPEHEHSPDAVQSQRLAHALNDMIKEFISMGSLVALIATSQSQQSLHPLLVSAQGVHIFQCVQHIQPPNQEQRCEILCNVIKNKLDCDINKFTDLDLQHVAKETGGFVARDFTVLVDRAIHSRLSRQSISTREKLVLTTLDFQKALRGFLPASLRSVNLHKPRDLGWDKIGGLHEVRQILMDTIQLPAKYPELFANLPIRQRTGILLYGPPGTGKTLLAGVIARESRMNFISVKGPELLSKYIGASEQAVRDIFIRAQAAKPCILFFDEFESIAPRRGHDNTGVTDRVVNQLLTQLDGVEGLQGVYVLAATSRPDLIDPALLRPGRLDKCVYCPPPDQVSRLEILNVLSDSLPLADDVDLQHVASVTDSFTGADLKALLYNAQLEALHGMLLSSGLQDGSSSSDSDLSLSSMVFLNHSSGSDDSAGDGECGLDQSLVSLEMSEILPDESKFNMYRLYFGSSYESELGNGTSSDLSSQCLSAPSSMTQDLPGVPGKDQLFSQPPVLRTASQEGCQELTQEQRDQLRADISIIKGRYRSQSGEDESMNQPGPIKTRLAISQSHLMTALGHTRPSISEDDWKNFAELYESFQNPKRRKNQSGTMFRPGQKVTLA

Target ORF information:

RefSeq Version	NM_000466.3
Organism	Homo sapiens(human)
Definition	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000466.3

ORF Insert Sequence:

ATGTGGGGCA GCGATCGCCT GGCGGGTGCT GGGGGAGGCG GGGCGGCAGT GACTGTGGCC 
TTCACCAACG CTCGCGACTG CTTCCTCCAC CTGCCGCGGC GTCTCGTGGC CCAGCTGCAT 
CTGCTGCAGA ATCAAGCTAT AGAAGTGGTC TGGAGTCACC AGCCTGCATT CTTGAGCTGG 
GTGGAAGGCA GGCATTTTAG TGATCAAGGT GAAAATGTGG CTGAAATTAA CAGACAAGTT 
GGTCAAAAAC TTGGACTCTC AAATGGGGGA CAGGTATTTC TCAAGCCATG TTCCCATGTG 
GTATCTTGTC AACAAGTTGA GGTGGAACCC CTCTCAGCAG ATGATTGGGA GATACTGGAG 
CTGCATGCTG TTTCCCTTGA ACAACATCTT CTAGATCAAA TTCGAATAGT TTTTCCAAAA 
GCCATTTTTC CTGTTTGGGT TGATCAACAA ACGTACATAT TTATCCAAAT TGTTGCACTA 
ATACCAGCTG CCTCTTATGG AAGGCTGGAA ACTGACACCA AACTCCTTAT TCAGCCAAAG 
ACACGCCGAG CCAAAGAGAA TACATTTTCA AAAGCTGATG CTGAATATAA AAAACTTCAT 
AGTTATGGAA GAGACCAGAA AGGAATGATG AAAGAACTTC AAACCAAGCA ACTTCAGTCA 
AATACTGTGG GAATCACTGA ATCTAATGAA AACGAGTCAG AGATTCCAGT TGACTCATCA 
TCAGTAGCAA GTTTATGGAC TATGATAGGA AGCATTTTTT CCTTTCAATC TGAGAAGAAA 
CAAGAGACAT CTTGGGGTTT AACTGAAATC AATGCATTCA AAAATATGCA GTCAAAGGTT 
GTTCCTCTAG ACAATATTTT CAGAGTATGC AAATCTCAAC CTCCTAGTAT ATATAACGCG 
TCAGCAACCT CTGTTTTTCA TAAACACTGT GCCATTCATG TATTTCCATG GGACCAGGAA 
TATTTTGATG TAGAGCCCAG CTTTACTGTG ACATATGGAA AGCTAGTTAA GCTACTTTCT 
CCAAAGCAAC AGCAAAGTAA AACAAAACAA AATGTGTTAT CACCTGAAAA AGAGAAGCAG 
ATGTCAGAGC CACTAGATCA AAAAAAAATT AGGTCAGATC ATAATGAAGA AGATGAGAAG 
GCCTGTGTGC TACAAGTAGT CTGGAATGGA CTTGAAGAAT TGAACAATGC CATCAAATAT 
ACCAAAAATG TAGAAGTTCT CCATCTTGGG AAAGTCTGGA TTCCAGATGA CCTGAGGAAG 
AGACTAAATA TAGAAATGCA TGCCGTAGTC AGGATAACTC CAGTGGAAGT TACCCCTAAA 
ATTCCAAGAT CTCTAAAGTT ACAACCTAGA GAGAATTTAC CTAAAGACAT AAGTGAAGAA 
GACATAAAAA CTGTATTTTA TTCATGGCTA CAGCAGTCTA CTACCACCAT GCTTCCTTTG 
GTAATATCAG AGGAAGAATT TATTAAGCTG GAAACTAAAG ATGGACTGAA GGAATTTTCT 
CTGAGTATAG TTCATTCTTG GGAAAAAGAA AAAGATAAAA ATATTTTTCT GTTGAGTCCC 
AATTTGCTGC AGAAGACTAC AATACAAGTC CTTCTAGATC CTATGGTAAA AGAAGAAAAC 
AGTGAGGAAA TTGACTTTAT TCTTCCTTTT TTAAAGCTGA GCTCTTTGGG AGGAGTGAAT 
TCCTTAGGCG TATCCTCCTT GGAGCACATC ACTCACAGCC TCCTGGGACG CCCTTTGTCT 
CGGCAGCTGA TGTCTCTTGT TGCAGGACTT AGGAATGGAG CTCTTTTACT CACAGGAGGA 
AAGGGAAGTG GAAAATCAAC TTTAGCCAAA GCAATCTGTA AAGAAGCATT TGACAAACTG 
GATGCCCATG TGGAGAGAGT TGACTGTAAA GCTTTACGAG GAAAAAGGCT TGAAAACATA 
CAAAAAACCC TAGAGGTGGC TTTCTCAGAG GCAGTGTGGA TGCAGCCATC TGTTGTCCTG 
CTGGATGACC TTGACCTCAT TGCTGGACTG CCTGCTGTCC CGGAACATGA GCACAGTCCT 
GATGCGGTGC AGAGCCAGCG GCTTGCTCAT GCTTTGAATG ATATGATAAA AGAGTTTATC 
TCCATGGGAA GTTTGGTTGC ACTGATTGCC ACAAGTCAGT CTCAGCAATC TCTACATCCT 
TTACTTGTTT CTGCTCAAGG AGTTCACATA TTTCAGTGCG TCCAACACAT TCAGCCTCCT 
AATCAGGAAC AAAGATGTGA AATTCTGTGT AATGTAATAA AAAATAAATT GGACTGTGAT 
ATAAACAAGT TCACCGATCT TGACCTGCAG CATGTAGCTA AAGAAACTGG CGGGTTTGTG 
GCTAGAGATT TTACAGTACT TGTGGATCGA GCCATACATT CTCGACTCTC TCGTCAGAGT 
ATATCCACCA GAGAAAAATT AGTTTTAACA ACATTGGACT TCCAAAAGGC TCTCCGCGGA 
TTTCTTCCTG CGTCTTTGCG AAGTGTCAAC CTGCATAAAC CTAGAGACCT GGGTTGGGAC 
AAGATTGGTG GGTTACATGA AGTTAGGCAG ATACTCATGG ATACTATCCA GTTACCTGCC 
AAGTATCCAG AATTATTTGC AAACTTGCCC ATACGACAAA GAACAGGAAT ACTGTTGTAT 
GGTCCGCCTG GAACAGGAAA AACCTTACTA GCTGGGGTAA TTGCACGAGA GAGTAGAATG 
AATTTTATAA GTGTCAAGGG GCCAGAGTTA CTCAGCAAAT ACATTGGAGC AAGTGAACAA 
GCTGTTCGGG ATATTTTTAT TAGAGCACAG GCTGCAAAGC CCTGCATTCT TTTCTTTGAT 
GAATTTGAAT CCATTGCTCC TCGGCGGGGT CATGATAATA CAGGAGTTAC AGACCGAGTA 
GTTAACCAGT TGCTGACTCA GTTGGATGGA GTAGAAGGCT TACAGGGTGT TTATGTATTG 
GCTGCTACTA GTCGCCCTGA CTTGATTGAC CCTGCCCTGC TTAGGCCTGG TCGACTAGAT 
AAATGTGTAT ACTGTCCTCC TCCTGATCAG GTGTCACGTC TTGAAATTTT AAATGTCCTC 
AGTGACTCTC TACCTCTGGC AGATGATGTT GACCTTCAGC ATGTAGCATC AGTAACTGAC 
TCCTTTACTG GAGCTGATCT GAAAGCTTTA CTTTACAATG CCCAATTGGA GGCCTTACAT 
GGAATGCTGC TCTCGAGTGG ACTCCAGGAT GGAAGTTCCA GCTCTGATAG TGACCTAAGT 
CTGTCTTCAA TGGTCTTTCT TAACCATAGC AGTGGCTCTG ACGATTCAGC TGGAGATGGA 
GAATGTGGCT TAGATCAGTC CCTTGTTTCT TTAGAGATGT CCGAGATCCT TCCAGATGAA 
TCAAAATTCA ATATGTACCG GCTCTACTTT GGAAGCTCTT ATGAATCAGA ACTTGGAAAT 
GGAACCTCTT CTGATTTGAG CTCACAATGT CTCTCTGCAC CAAGCTCCAT GACTCAGGAT 
TTGCCTGGAG TTCCTGGGAA AGACCAGTTG TTTTCACAGC CTCCAGTGTT AAGGACAGCT 
TCACAAGAGG GTTGCCAAGA ACTTACACAA GAACAAAGAG ATCAACTGAG GGCAGATATC 
AGTATTATCA AAGGCAGATA CCGGAGCCAA AGTGGAGAGG ACGAATCCAT GAACCAACCA 
GGACCAATCA AAACCAGACT GGCTATTAGT CAGTCACATT TAATGACTGC ACTTGGTCAC 
ACAAGACCAT CCATTAGTGA AGATGACTGG AAGAATTTTG CTGAGCTATA TGAAAGCTTT 
CAAAATCCAA AGAGGAGAAA AAATCAAAGT GGAACAATGT TTCGACCTGG ACAGAAAGTA 
ACTTTAGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEX1 ( XM_017012319.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867808.1 Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant X2, mRNA.

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CloneID	OHu41614
Clone ID Related Accession (Same CDS sequence)	XM_017012319.1 , XM_017012319.2
Accession Version	XM_017012319.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	peroxisome biogenesis factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTTG TTGCAGGACT TAGGAATGGA GCTCTTTTAC TCACAGGAGG AAAGGGAAGT 
GGAAAATCAA CTTTAGCCAA AGCAATCTGT AAAGAAGCAT TTGACAAACT GGATGCCCAT 
GTGGAGAGAG TTGACTGTAA AGCTTTACGA GGAAAAAGGC TTGAAAACAT ACAAAAAACC 
CTAGAGGTGG CTTTCTCAGA GGCAGTGTGG ATGCAGCCAT CTGTTGTCCT GCTGGATGAC 
CTTGACCTCA TTGCTGGACT GCCTGCTGTC CCGGAACATG AGCACAGTCC TGATGCGGTG 
CAGAGCCAGC GGCTTGCTCA TGCTTTGAAT GATATGATAA AAGAGTTTAT CTCCATGGGA 
AGTTTGGTTG CACTGATTGC CACAAGTCAG TCTCAGCAAT CTCTACATCC TTTACTTGTT 
TCTGCTCAAG GAGTTCACAT ATTTCAGTGC GTCCAACACA TTCAGCCTCC TAATCAGGAA 
CAAAGATGTG AAATTCTGTG TAATGTAATA AAAAATAAAT TGGACTGTGA TATAAACAAG 
TTCACCGATC TTGACCTGCA GCATGTAGCT AAAGAAACTG GCGGGTTTGT GGCTAGAGAT 
TTTACAGTAC TTGTGGATCG AGCCATACAT TCTCGACTCT CTCGTCAGAG TATATCCACC 
AGAGAAAAAT TAGTTTTAAC AACATTGGAC TTCCAAAAGG CTCTCCGCGG ATTTCTTCCT 
GCGTCTTTGC GAAGTGTCAA CCTGCATAAA CCTAGAGACC TGGGTTGGGA CAAGATTGGT 
GGGTTACATG AAGTTAGGCA GATACTCATG GATACTATCC AGTTACCTGC CAAGTATCCA 
GAATTATTTG CAAACTTGCC CATACGACAA AGAACAGGAA TACTGTTGTA TGGTCCGCCT 
GGAACAGGAA AAACCTTACT AGCTGGGGTA ATTGCACGAG AGAGTAGAAT GAATTTTATA 
AGTGTCAAGG GGCCAGAGTT ACTCAGCAAA TACATTGGAG CAAGTGAACA AGCTGTTCGG 
GATATTTTTA TTAGAGCACA GGCTGCAAAG CCCTGCATTC TTTTCTTTGA TGAATTTGAA 
TCCATTGCTC CTCGGCGGGG TCATGATAAT ACAGGAGTTA CAGACCGAGT AGTTAACCAG 
TTGCTGACTC AGTTGGATGG AGTAGAAGGC TTACAGGGTG TTTATGTATT GGCTGCTACT 
AGTCGCCCTG ACTTGATTGA CCCTGCCCTG CTTAGGCCTG GTCGACTAGA TAAATGTGTA 
TACTGTCCTC CTCCTGATCA GGTGTCACGT CTTGAAATTT TAAATGTCCT CAGTGACTCT 
CTACCTCTGG CAGATGATGT TGACCTTCAG CATGTAGCAT CAGTAACTGA CTCCTTTACT 
GGAGCTGATC TGAAAGCTTT ACTTTACAAT GCCCAATTGG AGGCCTTACA TGGAATGCTG 
CTCTCGAGTG GACTCCAGGA TGGAAGTTCC AGCTCTGATA GTGACCTAAG TCTGTCTTCA 
ATGGTCTTTC TTAACCATAG CAGTGGCTCT GACGATTCAG CTGGAGATGG AGAATGTGGC 
TTAGATCAGT CCCTTGTTTC TTTAGAGATG TCCGAGATCC TTCCAGATGA ATCAAAATTC 
AATATGTACC GGCTCTACTT TGGAAGCTCT TATGAATCAG AACTTGGAAA TGGAACCTCT 
TCTGATTTGA GCTCACAATG TCTCTCTGCA CCAAGCTCCA TGACTCAGGA TTTGCCTGGA 
GTTCCTGGGA AAGACCAGTT GTTTTCACAG CCTCCAGTGT TAAGGACAGC TTCACAAGAG 
GGTTGCCAAG AACTTACACA AGAACAAAGA GATCAACTGA GGGCAGATAT CAGTATTATC 
AAAGGCAGAT ACCGGAGCCA AAGTGGAGAG GACGAATCCA TGAACCAACC AGGACCAATC 
AAAACCAGAC TGGCTATTAG TCAGTCACAT TTAATGACTG CACTTGGTCA CACAAGACCA 
TCCATTAGTG AAGATGACTG GAAGAATTTT GCTGAGCTAT ATGAAAGCTT TCAAAATCCA 
AAGAGGAGAA AAAATCAAAG TGGAACAATG TTTCGACCTG GACAGAAAGT AACTTTAGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867808.1
CDS	766..2868
Translation	MSLVAGLRNGALLLTGGKGSGKSTLAKAICKEAFDKLDAHVERVDCKALRGKRLENIQKTLEVAFSEAVWMQPSVVLLDDLDLIAGLPAVPEHEHSPDAVQSQRLAHALNDMIKEFISMGSLVALIATSQSQQSLHPLLVSAQGVHIFQCVQHIQPPNQEQRCEILCNVIKNKLDCDINKFTDLDLQHVAKETGGFVARDFTVLVDRAIHSRLSRQSISTREKLVLTTLDFQKALRGFLPASLRSVNLHKPRDLGWDKIGGLHEVRQILMDTIQLPAKYPELFANLPIRQRTGILLYGPPGTGKTLLAGVIARESRMNFISVKGPELLSKYIGASEQAVRDIFIRAQAAKPCILFFDEFESIAPRRGHDNTGVTDRVVNQLLTQLDGVEGLQGVYVLAATSRPDLIDPALLRPGRLDKCVYCPPPDQVSRLEILNVLSDSLPLADDVDLQHVASVTDSFTGADLKALLYNAQLEALHGMLLSSGLQDGSSSSDSDLSLSSMVFLNHSSGSDDSAGDGECGLDQSLVSLEMSEILPDESKFNMYRLYFGSSYESELGNGTSSDLSSQCLSAPSSMTQDLPGVPGKDQLFSQPPVLRTASQEGCQELTQEQRDQLRADISIIKGRYRSQSGEDESMNQPGPIKTRLAISQSHLMTALGHTRPSISEDDWKNFAELYESFQNPKRRKNQSGTMFRPGQKVTLA

Target ORF information:

RefSeq Version	XM_017012319.1
Organism	Homo sapiens(human)
Definition	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012319.1

ORF Insert Sequence:

ATGTCTCTTG TTGCAGGACT TAGGAATGGA GCTCTTTTAC TCACAGGAGG AAAGGGAAGT 
GGAAAATCAA CTTTAGCCAA AGCAATCTGT AAAGAAGCAT TTGACAAACT GGATGCCCAT 
GTGGAGAGAG TTGACTGTAA AGCTTTACGA GGAAAAAGGC TTGAAAACAT ACAAAAAACC 
CTAGAGGTGG CTTTCTCAGA GGCAGTGTGG ATGCAGCCAT CTGTTGTCCT GCTGGATGAC 
CTTGACCTCA TTGCTGGACT GCCTGCTGTC CCGGAACATG AGCACAGTCC TGATGCGGTG 
CAGAGCCAGC GGCTTGCTCA TGCTTTGAAT GATATGATAA AAGAGTTTAT CTCCATGGGA 
AGTTTGGTTG CACTGATTGC CACAAGTCAG TCTCAGCAAT CTCTACATCC TTTACTTGTT 
TCTGCTCAAG GAGTTCACAT ATTTCAGTGC GTCCAACACA TTCAGCCTCC TAATCAGGAA 
CAAAGATGTG AAATTCTGTG TAATGTAATA AAAAATAAAT TGGACTGTGA TATAAACAAG 
TTCACCGATC TTGACCTGCA GCATGTAGCT AAAGAAACTG GCGGGTTTGT GGCTAGAGAT 
TTTACAGTAC TTGTGGATCG AGCCATACAT TCTCGACTCT CTCGTCAGAG TATATCCACC 
AGAGAAAAAT TAGTTTTAAC AACATTGGAC TTCCAAAAGG CTCTCCGCGG ATTTCTTCCT 
GCGTCTTTGC GAAGTGTCAA CCTGCATAAA CCTAGAGACC TGGGTTGGGA CAAGATTGGT 
GGGTTACATG AAGTTAGGCA GATACTCATG GATACTATCC AGTTACCTGC CAAGTATCCA 
GAATTATTTG CAAACTTGCC CATACGACAA AGAACAGGAA TACTGTTGTA TGGTCCGCCT 
GGAACAGGAA AAACCTTACT AGCTGGGGTA ATTGCACGAG AGAGTAGAAT GAATTTTATA 
AGTGTCAAGG GGCCAGAGTT ACTCAGCAAA TACATTGGAG CAAGTGAACA AGCTGTTCGG 
GATATTTTTA TTAGAGCACA GGCTGCAAAG CCCTGCATTC TTTTCTTTGA TGAATTTGAA 
TCCATTGCTC CTCGGCGGGG TCATGATAAT ACAGGAGTTA CAGACCGAGT AGTTAACCAG 
TTGCTGACTC AGTTGGATGG AGTAGAAGGC TTACAGGGTG TTTATGTATT GGCTGCTACT 
AGTCGCCCTG ACTTGATTGA CCCTGCCCTG CTTAGGCCTG GTCGACTAGA TAAATGTGTA 
TACTGTCCTC CTCCTGATCA GGTGTCACGT CTTGAAATTT TAAATGTCCT CAGTGACTCT 
CTACCTCTGG CAGATGATGT TGACCTTCAG CATGTAGCAT CAGTAACTGA CTCCTTTACT 
GGAGCTGATC TGAAAGCTTT ACTTTACAAT GCCCAATTGG AGGCCTTACA TGGAATGCTG 
CTCTCGAGTG GACTCCAGGA TGGAAGTTCC AGCTCTGATA GTGACCTAAG TCTGTCTTCA 
ATGGTCTTTC TTAACCATAG CAGTGGCTCT GACGATTCAG CTGGAGATGG AGAATGTGGC 
TTAGATCAGT CCCTTGTTTC TTTAGAGATG TCCGAGATCC TTCCAGATGA ATCAAAATTC 
AATATGTACC GGCTCTACTT TGGAAGCTCT TATGAATCAG AACTTGGAAA TGGAACCTCT 
TCTGATTTGA GCTCACAATG TCTCTCTGCA CCAAGCTCCA TGACTCAGGA TTTGCCTGGA 
GTTCCTGGGA AAGACCAGTT GTTTTCACAG CCTCCAGTGT TAAGGACAGC TTCACAAGAG 
GGTTGCCAAG AACTTACACA AGAACAAAGA GATCAACTGA GGGCAGATAT CAGTATTATC 
AAAGGCAGAT ACCGGAGCCA AAGTGGAGAG GACGAATCCA TGAACCAACC AGGACCAATC 
AAAACCAGAC TGGCTATTAG TCAGTCACAT TTAATGACTG CACTTGGTCA CACAAGACCA 
TCCATTAGTG AAGATGACTG GAAGAATTTT GCTGAGCTAT ATGAAAGCTT TCAAAATCCA 
AAGAGGAGAA AAAATCAAAG TGGAACAATG TTTCGACCTG GACAGAAAGT AACTTTAGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEX1 ( XM_017012319.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867808.1 Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant X2, mRNA.

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CloneID	OHu41614
Clone ID Related Accession (Same CDS sequence)	XM_017012319.1 , XM_017012319.2
Accession Version	XM_017012319.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	peroxisome biogenesis factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017012319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTTG TTGCAGGACT TAGGAATGGA GCTCTTTTAC TCACAGGAGG AAAGGGAAGT 
GGAAAATCAA CTTTAGCCAA AGCAATCTGT AAAGAAGCAT TTGACAAACT GGATGCCCAT 
GTGGAGAGAG TTGACTGTAA AGCTTTACGA GGAAAAAGGC TTGAAAACAT ACAAAAAACC 
CTAGAGGTGG CTTTCTCAGA GGCAGTGTGG ATGCAGCCAT CTGTTGTCCT GCTGGATGAC 
CTTGACCTCA TTGCTGGACT GCCTGCTGTC CCGGAACATG AGCACAGTCC TGATGCGGTG 
CAGAGCCAGC GGCTTGCTCA TGCTTTGAAT GATATGATAA AAGAGTTTAT CTCCATGGGA 
AGTTTGGTTG CACTGATTGC CACAAGTCAG TCTCAGCAAT CTCTACATCC TTTACTTGTT 
TCTGCTCAAG GAGTTCACAT ATTTCAGTGC GTCCAACACA TTCAGCCTCC TAATCAGGAA 
CAAAGATGTG AAATTCTGTG TAATGTAATA AAAAATAAAT TGGACTGTGA TATAAACAAG 
TTCACCGATC TTGACCTGCA GCATGTAGCT AAAGAAACTG GCGGGTTTGT GGCTAGAGAT 
TTTACAGTAC TTGTGGATCG AGCCATACAT TCTCGACTCT CTCGTCAGAG TATATCCACC 
AGAGAAAAAT TAGTTTTAAC AACATTGGAC TTCCAAAAGG CTCTCCGCGG ATTTCTTCCT 
GCGTCTTTGC GAAGTGTCAA CCTGCATAAA CCTAGAGACC TGGGTTGGGA CAAGATTGGT 
GGGTTACATG AAGTTAGGCA GATACTCATG GATACTATCC AGTTACCTGC CAAGTATCCA 
GAATTATTTG CAAACTTGCC CATACGACAA AGAACAGGAA TACTGTTGTA TGGTCCGCCT 
GGAACAGGAA AAACCTTACT AGCTGGGGTA ATTGCACGAG AGAGTAGAAT GAATTTTATA 
AGTGTCAAGG GGCCAGAGTT ACTCAGCAAA TACATTGGAG CAAGTGAACA AGCTGTTCGG 
GATATTTTTA TTAGAGCACA GGCTGCAAAG CCCTGCATTC TTTTCTTTGA TGAATTTGAA 
TCCATTGCTC CTCGGCGGGG TCATGATAAT ACAGGAGTTA CAGACCGAGT AGTTAACCAG 
TTGCTGACTC AGTTGGATGG AGTAGAAGGC TTACAGGGTG TTTATGTATT GGCTGCTACT 
AGTCGCCCTG ACTTGATTGA CCCTGCCCTG CTTAGGCCTG GTCGACTAGA TAAATGTGTA 
TACTGTCCTC CTCCTGATCA GGTGTCACGT CTTGAAATTT TAAATGTCCT CAGTGACTCT 
CTACCTCTGG CAGATGATGT TGACCTTCAG CATGTAGCAT CAGTAACTGA CTCCTTTACT 
GGAGCTGATC TGAAAGCTTT ACTTTACAAT GCCCAATTGG AGGCCTTACA TGGAATGCTG 
CTCTCGAGTG GACTCCAGGA TGGAAGTTCC AGCTCTGATA GTGACCTAAG TCTGTCTTCA 
ATGGTCTTTC TTAACCATAG CAGTGGCTCT GACGATTCAG CTGGAGATGG AGAATGTGGC 
TTAGATCAGT CCCTTGTTTC TTTAGAGATG TCCGAGATCC TTCCAGATGA ATCAAAATTC 
AATATGTACC GGCTCTACTT TGGAAGCTCT TATGAATCAG AACTTGGAAA TGGAACCTCT 
TCTGATTTGA GCTCACAATG TCTCTCTGCA CCAAGCTCCA TGACTCAGGA TTTGCCTGGA 
GTTCCTGGGA AAGACCAGTT GTTTTCACAG CCTCCAGTGT TAAGGACAGC TTCACAAGAG 
GGTTGCCAAG AACTTACACA AGAACAAAGA GATCAACTGA GGGCAGATAT CAGTATTATC 
AAAGGCAGAT ACCGGAGCCA AAGTGGAGAG GACGAATCCA TGAACCAACC AGGACCAATC 
AAAACCAGAC TGGCTATTAG TCAGTCACAT TTAATGACTG CACTTGGTCA CACAAGACCA 
TCCATTAGTG AAGATGACTG GAAGAATTTT GCTGAGCTAT ATGAAAGCTT TCAAAATCCA 
AAGAGGAGAA AAAATCAAAG TGGAACAATG TTTCGACCTG GACAGAAAGT AACTTTAGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867808.1
CDS	777..2879
Translation	MSLVAGLRNGALLLTGGKGSGKSTLAKAICKEAFDKLDAHVERVDCKALRGKRLENIQKTLEVAFSEAVWMQPSVVLLDDLDLIAGLPAVPEHEHSPDAVQSQRLAHALNDMIKEFISMGSLVALIATSQSQQSLHPLLVSAQGVHIFQCVQHIQPPNQEQRCEILCNVIKNKLDCDINKFTDLDLQHVAKETGGFVARDFTVLVDRAIHSRLSRQSISTREKLVLTTLDFQKALRGFLPASLRSVNLHKPRDLGWDKIGGLHEVRQILMDTIQLPAKYPELFANLPIRQRTGILLYGPPGTGKTLLAGVIARESRMNFISVKGPELLSKYIGASEQAVRDIFIRAQAAKPCILFFDEFESIAPRRGHDNTGVTDRVVNQLLTQLDGVEGLQGVYVLAATSRPDLIDPALLRPGRLDKCVYCPPPDQVSRLEILNVLSDSLPLADDVDLQHVASVTDSFTGADLKALLYNAQLEALHGMLLSSGLQDGSSSSDSDLSLSSMVFLNHSSGSDDSAGDGECGLDQSLVSLEMSEILPDESKFNMYRLYFGSSYESELGNGTSSDLSSQCLSAPSSMTQDLPGVPGKDQLFSQPPVLRTASQEGCQELTQEQRDQLRADISIIKGRYRSQSGEDESMNQPGPIKTRLAISQSHLMTALGHTRPSISEDDWKNFAELYESFQNPKRRKNQSGTMFRPGQKVTLA

Target ORF information:

RefSeq Version	XM_017012319.2
Organism	Homo sapiens(human)
Definition	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012319.2

ORF Insert Sequence:

ATGTCTCTTG TTGCAGGACT TAGGAATGGA GCTCTTTTAC TCACAGGAGG AAAGGGAAGT 
GGAAAATCAA CTTTAGCCAA AGCAATCTGT AAAGAAGCAT TTGACAAACT GGATGCCCAT 
GTGGAGAGAG TTGACTGTAA AGCTTTACGA GGAAAAAGGC TTGAAAACAT ACAAAAAACC 
CTAGAGGTGG CTTTCTCAGA GGCAGTGTGG ATGCAGCCAT CTGTTGTCCT GCTGGATGAC 
CTTGACCTCA TTGCTGGACT GCCTGCTGTC CCGGAACATG AGCACAGTCC TGATGCGGTG 
CAGAGCCAGC GGCTTGCTCA TGCTTTGAAT GATATGATAA AAGAGTTTAT CTCCATGGGA 
AGTTTGGTTG CACTGATTGC CACAAGTCAG TCTCAGCAAT CTCTACATCC TTTACTTGTT 
TCTGCTCAAG GAGTTCACAT ATTTCAGTGC GTCCAACACA TTCAGCCTCC TAATCAGGAA 
CAAAGATGTG AAATTCTGTG TAATGTAATA AAAAATAAAT TGGACTGTGA TATAAACAAG 
TTCACCGATC TTGACCTGCA GCATGTAGCT AAAGAAACTG GCGGGTTTGT GGCTAGAGAT 
TTTACAGTAC TTGTGGATCG AGCCATACAT TCTCGACTCT CTCGTCAGAG TATATCCACC 
AGAGAAAAAT TAGTTTTAAC AACATTGGAC TTCCAAAAGG CTCTCCGCGG ATTTCTTCCT 
GCGTCTTTGC GAAGTGTCAA CCTGCATAAA CCTAGAGACC TGGGTTGGGA CAAGATTGGT 
GGGTTACATG AAGTTAGGCA GATACTCATG GATACTATCC AGTTACCTGC CAAGTATCCA 
GAATTATTTG CAAACTTGCC CATACGACAA AGAACAGGAA TACTGTTGTA TGGTCCGCCT 
GGAACAGGAA AAACCTTACT AGCTGGGGTA ATTGCACGAG AGAGTAGAAT GAATTTTATA 
AGTGTCAAGG GGCCAGAGTT ACTCAGCAAA TACATTGGAG CAAGTGAACA AGCTGTTCGG 
GATATTTTTA TTAGAGCACA GGCTGCAAAG CCCTGCATTC TTTTCTTTGA TGAATTTGAA 
TCCATTGCTC CTCGGCGGGG TCATGATAAT ACAGGAGTTA CAGACCGAGT AGTTAACCAG 
TTGCTGACTC AGTTGGATGG AGTAGAAGGC TTACAGGGTG TTTATGTATT GGCTGCTACT 
AGTCGCCCTG ACTTGATTGA CCCTGCCCTG CTTAGGCCTG GTCGACTAGA TAAATGTGTA 
TACTGTCCTC CTCCTGATCA GGTGTCACGT CTTGAAATTT TAAATGTCCT CAGTGACTCT 
CTACCTCTGG CAGATGATGT TGACCTTCAG CATGTAGCAT CAGTAACTGA CTCCTTTACT 
GGAGCTGATC TGAAAGCTTT ACTTTACAAT GCCCAATTGG AGGCCTTACA TGGAATGCTG 
CTCTCGAGTG GACTCCAGGA TGGAAGTTCC AGCTCTGATA GTGACCTAAG TCTGTCTTCA 
ATGGTCTTTC TTAACCATAG CAGTGGCTCT GACGATTCAG CTGGAGATGG AGAATGTGGC 
TTAGATCAGT CCCTTGTTTC TTTAGAGATG TCCGAGATCC TTCCAGATGA ATCAAAATTC 
AATATGTACC GGCTCTACTT TGGAAGCTCT TATGAATCAG AACTTGGAAA TGGAACCTCT 
TCTGATTTGA GCTCACAATG TCTCTCTGCA CCAAGCTCCA TGACTCAGGA TTTGCCTGGA 
GTTCCTGGGA AAGACCAGTT GTTTTCACAG CCTCCAGTGT TAAGGACAGC TTCACAAGAG 
GGTTGCCAAG AACTTACACA AGAACAAAGA GATCAACTGA GGGCAGATAT CAGTATTATC 
AAAGGCAGAT ACCGGAGCCA AAGTGGAGAG GACGAATCCA TGAACCAACC AGGACCAATC 
AAAACCAGAC TGGCTATTAG TCAGTCACAT TTAATGACTG CACTTGGTCA CACAAGACCA 
TCCATTAGTG AAGATGACTG GAAGAATTTT GCTGAGCTAT ATGAAAGCTT TCAAAATCCA 
AAGAGGAGAA AAAATCAAAG TGGAACAATG TTTCGACCTG GACAGAAAGT AACTTTAGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEX1 ( NM_001282678.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269607.1 Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 3, mRNA.

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CloneID	OHu16877
Clone ID Related Accession (Same CDS sequence)	NM_001282678.1 , NM_001282678.2
Accession Version	NM_001282678.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	peroxisome biogenesis factor 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA313282.1, DC370317.1, BC035575.1, DB461678.1 and AA490966.1. On Sep 18, 2013 this sequence version replaced XM_005250431.1. Transcript Variant: This variant (3) uses an alternate donor splice site in the 5' region, which results in translation initiation from an in-frame downstream start codon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.102027.1, SRR1803616.7546.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGAAAG AACTTCAAAC CAAGCAACTT CAGTCAAATA CTGTGGGAAT CACTGAATCT 
AATGAAAACG AGTCAGAGAT TCCAGTTGAC TCATCATCAG TAGCAAGTTT ATGGACTATG 
ATAGGAAGCA TTTTTTCCTT TCAATCTGAG AAGAAACAAG AGACATCTTG GGGTTTAACT 
GAAATCAATG CATTCAAAAA TATGCAGTCA AAGGTTGTTC CTCTAGACAA TATTTTCAGA 
GTATGCAAAT CTCAACCTCC TAGTATATAT AACGCGTCAG CAACCTCTGT TTTTCATAAA 
CACTGTGCCA TTCATGTATT TCCATGGGAC CAGGAATATT TTGATGTAGA GCCCAGCTTT 
ACTGTGACAT ATGGAAAGCT AGTTAAGCTA CTTTCTCCAA AGCAACAGCA AAGTAAAACA 
AAACAAAATG TGTTATCACC TGAAAAAGAG AAGCAGATGT CAGAGCCACT AGATCAAAAA 
AAAATTAGGT CAGATCATAA TGAAGAAGAT GAGAAGGCCT GTGTGCTACA AGTAGTCTGG 
AATGGACTTG AAGAATTGAA CAATGCCATC AAATATACCA AAAATGTAGA AGTTCTCCAT 
CTTGGGAAAG TCTGGATTCC AGATGACCTG AGGAAGAGAC TAAATATAGA AATGCATGCC 
GTAGTCAGGA TAACTCCAGT GGAAGTTACC CCTAAAATTC CAAGATCTCT AAAGTTACAA 
CCTAGAGAGA ATTTACCTAA AGACATAAGT GAAGAAGACA TAAAAACTGT ATTTTATTCA 
TGGCTACAGC AGTCTACTAC CACCATGCTT CCTTTGGTAA TATCAGAGGA AGAATTTATT 
AAGCTGGAAA CTAAAGATGG ACTGAAGGAA TTTTCTCTGA GTATAGTTCA TTCTTGGGAA 
AAAGAAAAAG ATAAAAATAT TTTTCTGTTG AGTCCCAATT TGCTGCAGAA GACTACAATA 
CAAGTCCTTC TAGATCCTAT GGTAAAAGAA GAAAACAGTG AGGAAATTGA CTTTATTCTT 
CCTTTTTTAA AGCTGAGCTC TTTGGGAGGA GTGAATTCCT TAGGCGTATC CTCCTTGGAG 
CACATCACTC ACAGCCTCCT GGGACGCCCT TTGTCTCGGC AGCTGATGTC TCTTGTTGCA 
GGACTTAGGA ATGGAGCTCT TTTACTCACA GGAGGAAAGG GAAGTGGAAA ATCAACTTTA 
GCCAAAGCAA TCTGTAAAGA AGCATTTGAC AAACTGGATG CCCATGTGGA GAGAGTTGAC 
TGTAAAGCTT TACGAGGAAA AAGGCTTGAA AACATACAAA AAACCCTAGA GGTGGCTTTC 
TCAGAGGCAG TGTGGATGCA GCCATCTGTT GTCCTGCTGG ATGACCTTGA CCTCATTGCT 
GGACTGCCTG CTGTCCCGGA ACATGAGCAC AGTCCTGATG CGGTGCAGAG CCAGCGGCTT 
GCTCATGCTT TGAATGATAT GATAAAAGAG TTTATCTCCA TGGGAAGTTT GGTTGCACTG 
ATTGCCACAA GTCAGTCTCA GCAATCTCTA CATCCTTTAC TTGTTTCTGC TCAAGGAGTT 
CACATATTTC AGTGCGTCCA ACACATTCAG CCTCCTAATC AGGAACAAAG ATGTGAAATT 
CTGTGTAATG TAATAAAAAA TAAATTGGAC TGTGATATAA ACAAGTTCAC CGATCTTGAC 
CTGCAGCATG TAGCTAAAGA AACTGGCGGG TTTGTGGCTA GAGATTTTAC AGTACTTGTG 
GATCGAGCCA TACATTCTCG ACTCTCTCGT CAGAGTATAT CCACCAGAGA AAAATTAGTT 
TTAACAACAT TGGACTTCCA AAAGGCTCTC CGCGGATTTC TTCCTGCGTC TTTGCGAAGT 
GTCAACCTGC ATAAACCTAG AGACCTGGGT TGGGACAAGA TTGGTGGGTT ACATGAAGTT 
AGGCAGATAC TCATGGATAC TATCCAGTTA CCTGCCAAGT ATCCAGAATT ATTTGCAAAC 
TTGCCCATAC GACAAAGAAC AGGAATACTG TTGTATGGTC CGCCTGGAAC AGGAAAAACC 
TTACTAGCTG GGGTAATTGC ACGAGAGAGT AGAATGAATT TTATAAGTGT CAAGGGGCCA 
GAGTTACTCA GCAAATACAT TGGAGCAAGT GAACAAGCTG TTCGGGATAT TTTTATTAGA 
GCACAGGCTG CAAAGCCCTG CATTCTTTTC TTTGATGAAT TTGAATCCAT TGCTCCTCGG 
CGGGGTCATG ATAATACAGG AGTTACAGAC CGAGTAGTTA ACCAGTTGCT GACTCAGTTG 
GATGGAGTAG AAGGCTTACA GGGTGTTTAT GTATTGGCTG CTACTAGTCG CCCTGACTTG 
ATTGACCCTG CCCTGCTTAG GCCTGGTCGA CTAGATAAAT GTGTATACTG TCCTCCTCCT 
GATCAGGTGT CACGTCTTGA AATTTTAAAT GTCCTCAGTG ACTCTCTACC TCTGGCAGAT 
GATGTTGACC TTCAGCATGT AGCATCAGTA ACTGACTCCT TTACTGGAGC TGATCTGAAA 
GCTTTACTTT ACAATGCCCA ATTGGAGGCC TTACATGGAA TGCTGCTCTC GAGTGGACTC 
CAGGATGGAA GTTCCAGCTC TGATAGTGAC CTAAGTCTGT CTTCAATGGT CTTTCTTAAC 
CATAGCAGTG GCTCTGACGA TTCAGCTGGA GATGGAGAAT GTGGCTTAGA TCAGTCCCTT 
GTTTCTTTAG AGATGTCCGA GATCCTTCCA GATGAATCAA AATTCAATAT GTACCGGCTC 
TACTTTGGAA GCTCTTATGA ATCAGAACTT GGAAATGGAA CCTCTTCTGA TTTGAGCTCA 
CAATGTCTCT CTGCACCAAG CTCCATGACT CAGGATTTGC CTGGAGTTCC TGGGAAAGAC 
CAGTTGTTTT CACAGCCTCC AGTGTTAAGG ACAGCTTCAC AAGAGGGTTG CCAAGAACTT 
ACACAAGAAC AAAGAGATCA ACTGAGGGCA GATATCAGTA TTATCAAAGG CAGATACCGG 
AGCCAAAGTG GAGAGGACGA ATCCATGAAC CAACCAGGAC CAATCAAAAC CAGACTGGCT 
ATTAGTCAGT CACATTTAAT GACTGCACTT GGTCACACAA GACCATCCAT TAGTGAAGAT 
GACTGGAAGA ATTTTGCTGA GCTATATGAA AGCTTTCAAA ATCCAAAGAG GAGAAAAAAT 
CAAAGTGGAA CAATGTTTCG ACCTGGACAG AAAGTAACTT TAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269607.1
CDS	756..3983
Translation	MMKELQTKQLQSNTVGITESNENESEIPVDSSSVASLWTMIGSIFSFQSEKKQETSWGLTEINAFKNMQSKVVPLDNIFRVCKSQPPSIYNASATSVFHKHCAIHVFPWDQEYFDVEPSFTVTYGKLVKLLSPKQQQSKTKQNVLSPEKEKQMSEPLDQKKIRSDHNEEDEKACVLQVVWNGLEELNNAIKYTKNVEVLHLGKVWIPDDLRKRLNIEMHAVVRITPVEVTPKIPRSLKLQPRENLPKDISEEDIKTVFYSWLQQSTTTMLPLVISEEEFIKLETKDGLKEFSLSIVHSWEKEKDKNIFLLSPNLLQKTTIQVLLDPMVKEENSEEIDFILPFLKLSSLGGVNSLGVSSLEHITHSLLGRPLSRQLMSLVAGLRNGALLLTGGKGSGKSTLAKAICKEAFDKLDAHVERVDCKALRGKRLENIQKTLEVAFSEAVWMQPSVVLLDDLDLIAGLPAVPEHEHSPDAVQSQRLAHALNDMIKEFISMGSLVALIATSQSQQSLHPLLVSAQGVHIFQCVQHIQPPNQEQRCEILCNVIKNKLDCDINKFTDLDLQHVAKETGGFVARDFTVLVDRAIHSRLSRQSISTREKLVLTTLDFQKALRGFLPASLRSVNLHKPRDLGWDKIGGLHEVRQILMDTIQLPAKYPELFANLPIRQRTGILLYGPPGTGKTLLAGVIARESRMNFISVKGPELLSKYIGASEQAVRDIFIRAQAAKPCILFFDEFESIAPRRGHDNTGVTDRVVNQLLTQLDGVEGLQGVYVLAATSRPDLIDPALLRPGRLDKCVYCPPPDQVSRLEILNVLSDSLPLADDVDLQHVASVTDSFTGADLKALLYNAQLEALHGMLLSSGLQDGSSSSDSDLSLSSMVFLNHSSGSDDSAGDGECGLDQSLVSLEMSEILPDESKFNMYRLYFGSSYESELGNGTSSDLSSQCLSAPSSMTQDLPGVPGKDQLFSQPPVLRTASQEGCQELTQEQRDQLRADISIIKGRYRSQSGEDESMNQPGPIKTRLAISQSHLMTALGHTRPSISEDDWKNFAELYESFQNPKRRKNQSGTMFRPGQKVTLA

Target ORF information:

RefSeq Version	NM_001282678.1
Organism	Homo sapiens(human)
Definition	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282678.1

ORF Insert Sequence:

ATGATGAAAG AACTTCAAAC CAAGCAACTT CAGTCAAATA CTGTGGGAAT CACTGAATCT 
AATGAAAACG AGTCAGAGAT TCCAGTTGAC TCATCATCAG TAGCAAGTTT ATGGACTATG 
ATAGGAAGCA TTTTTTCCTT TCAATCTGAG AAGAAACAAG AGACATCTTG GGGTTTAACT 
GAAATCAATG CATTCAAAAA TATGCAGTCA AAGGTTGTTC CTCTAGACAA TATTTTCAGA 
GTATGCAAAT CTCAACCTCC TAGTATATAT AACGCGTCAG CAACCTCTGT TTTTCATAAA 
CACTGTGCCA TTCATGTATT TCCATGGGAC CAGGAATATT TTGATGTAGA GCCCAGCTTT 
ACTGTGACAT ATGGAAAGCT AGTTAAGCTA CTTTCTCCAA AGCAACAGCA AAGTAAAACA 
AAACAAAATG TGTTATCACC TGAAAAAGAG AAGCAGATGT CAGAGCCACT AGATCAAAAA 
AAAATTAGGT CAGATCATAA TGAAGAAGAT GAGAAGGCCT GTGTGCTACA AGTAGTCTGG 
AATGGACTTG AAGAATTGAA CAATGCCATC AAATATACCA AAAATGTAGA AGTTCTCCAT 
CTTGGGAAAG TCTGGATTCC AGATGACCTG AGGAAGAGAC TAAATATAGA AATGCATGCC 
GTAGTCAGGA TAACTCCAGT GGAAGTTACC CCTAAAATTC CAAGATCTCT AAAGTTACAA 
CCTAGAGAGA ATTTACCTAA AGACATAAGT GAAGAAGACA TAAAAACTGT ATTTTATTCA 
TGGCTACAGC AGTCTACTAC CACCATGCTT CCTTTGGTAA TATCAGAGGA AGAATTTATT 
AAGCTGGAAA CTAAAGATGG ACTGAAGGAA TTTTCTCTGA GTATAGTTCA TTCTTGGGAA 
AAAGAAAAAG ATAAAAATAT TTTTCTGTTG AGTCCCAATT TGCTGCAGAA GACTACAATA 
CAAGTCCTTC TAGATCCTAT GGTAAAAGAA GAAAACAGTG AGGAAATTGA CTTTATTCTT 
CCTTTTTTAA AGCTGAGCTC TTTGGGAGGA GTGAATTCCT TAGGCGTATC CTCCTTGGAG 
CACATCACTC ACAGCCTCCT GGGACGCCCT TTGTCTCGGC AGCTGATGTC TCTTGTTGCA 
GGACTTAGGA ATGGAGCTCT TTTACTCACA GGAGGAAAGG GAAGTGGAAA ATCAACTTTA 
GCCAAAGCAA TCTGTAAAGA AGCATTTGAC AAACTGGATG CCCATGTGGA GAGAGTTGAC 
TGTAAAGCTT TACGAGGAAA AAGGCTTGAA AACATACAAA AAACCCTAGA GGTGGCTTTC 
TCAGAGGCAG TGTGGATGCA GCCATCTGTT GTCCTGCTGG ATGACCTTGA CCTCATTGCT 
GGACTGCCTG CTGTCCCGGA ACATGAGCAC AGTCCTGATG CGGTGCAGAG CCAGCGGCTT 
GCTCATGCTT TGAATGATAT GATAAAAGAG TTTATCTCCA TGGGAAGTTT GGTTGCACTG 
ATTGCCACAA GTCAGTCTCA GCAATCTCTA CATCCTTTAC TTGTTTCTGC TCAAGGAGTT 
CACATATTTC AGTGCGTCCA ACACATTCAG CCTCCTAATC AGGAACAAAG ATGTGAAATT 
CTGTGTAATG TAATAAAAAA TAAATTGGAC TGTGATATAA ACAAGTTCAC CGATCTTGAC 
CTGCAGCATG TAGCTAAAGA AACTGGCGGG TTTGTGGCTA GAGATTTTAC AGTACTTGTG 
GATCGAGCCA TACATTCTCG ACTCTCTCGT CAGAGTATAT CCACCAGAGA AAAATTAGTT 
TTAACAACAT TGGACTTCCA AAAGGCTCTC CGCGGATTTC TTCCTGCGTC TTTGCGAAGT 
GTCAACCTGC ATAAACCTAG AGACCTGGGT TGGGACAAGA TTGGTGGGTT ACATGAAGTT 
AGGCAGATAC TCATGGATAC TATCCAGTTA CCTGCCAAGT ATCCAGAATT ATTTGCAAAC 
TTGCCCATAC GACAAAGAAC AGGAATACTG TTGTATGGTC CGCCTGGAAC AGGAAAAACC 
TTACTAGCTG GGGTAATTGC ACGAGAGAGT AGAATGAATT TTATAAGTGT CAAGGGGCCA 
GAGTTACTCA GCAAATACAT TGGAGCAAGT GAACAAGCTG TTCGGGATAT TTTTATTAGA 
GCACAGGCTG CAAAGCCCTG CATTCTTTTC TTTGATGAAT TTGAATCCAT TGCTCCTCGG 
CGGGGTCATG ATAATACAGG AGTTACAGAC CGAGTAGTTA ACCAGTTGCT GACTCAGTTG 
GATGGAGTAG AAGGCTTACA GGGTGTTTAT GTATTGGCTG CTACTAGTCG CCCTGACTTG 
ATTGACCCTG CCCTGCTTAG GCCTGGTCGA CTAGATAAAT GTGTATACTG TCCTCCTCCT 
GATCAGGTGT CACGTCTTGA AATTTTAAAT GTCCTCAGTG ACTCTCTACC TCTGGCAGAT 
GATGTTGACC TTCAGCATGT AGCATCAGTA ACTGACTCCT TTACTGGAGC TGATCTGAAA 
GCTTTACTTT ACAATGCCCA ATTGGAGGCC TTACATGGAA TGCTGCTCTC GAGTGGACTC 
CAGGATGGAA GTTCCAGCTC TGATAGTGAC CTAAGTCTGT CTTCAATGGT CTTTCTTAAC 
CATAGCAGTG GCTCTGACGA TTCAGCTGGA GATGGAGAAT GTGGCTTAGA TCAGTCCCTT 
GTTTCTTTAG AGATGTCCGA GATCCTTCCA GATGAATCAA AATTCAATAT GTACCGGCTC 
TACTTTGGAA GCTCTTATGA ATCAGAACTT GGAAATGGAA CCTCTTCTGA TTTGAGCTCA 
CAATGTCTCT CTGCACCAAG CTCCATGACT CAGGATTTGC CTGGAGTTCC TGGGAAAGAC 
CAGTTGTTTT CACAGCCTCC AGTGTTAAGG ACAGCTTCAC AAGAGGGTTG CCAAGAACTT 
ACACAAGAAC AAAGAGATCA ACTGAGGGCA GATATCAGTA TTATCAAAGG CAGATACCGG 
AGCCAAAGTG GAGAGGACGA ATCCATGAAC CAACCAGGAC CAATCAAAAC CAGACTGGCT 
ATTAGTCAGT CACATTTAAT GACTGCACTT GGTCACACAA GACCATCCAT TAGTGAAGAT 
GACTGGAAGA ATTTTGCTGA GCTATATGAA AGCTTTCAAA ATCCAAAGAG GAGAAAAAAT 
CAAAGTGGAA CAATGTTTCG ACCTGGACAG AAAGTAACTT TAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEX1 ( NM_001282678.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269607.1 Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu16877
Clone ID Related Accession (Same CDS sequence)	NM_001282678.1 , NM_001282678.2
Accession Version	NM_001282678.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	peroxisome biogenesis factor 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA313282.1, DC370317.1, BC035575.1 and DB461678.1. On May 31, 2019 this sequence version replaced NM_001282678.1. Transcript Variant: This variant (3) uses an alternate donor splice site in the 5' region, which results in translation initiation from an in-frame downstream start codon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.102027.1, SRR1803616.7546.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGAAAG AACTTCAAAC CAAGCAACTT CAGTCAAATA CTGTGGGAAT CACTGAATCT 
AATGAAAACG AGTCAGAGAT TCCAGTTGAC TCATCATCAG TAGCAAGTTT ATGGACTATG 
ATAGGAAGCA TTTTTTCCTT TCAATCTGAG AAGAAACAAG AGACATCTTG GGGTTTAACT 
GAAATCAATG CATTCAAAAA TATGCAGTCA AAGGTTGTTC CTCTAGACAA TATTTTCAGA 
GTATGCAAAT CTCAACCTCC TAGTATATAT AACGCGTCAG CAACCTCTGT TTTTCATAAA 
CACTGTGCCA TTCATGTATT TCCATGGGAC CAGGAATATT TTGATGTAGA GCCCAGCTTT 
ACTGTGACAT ATGGAAAGCT AGTTAAGCTA CTTTCTCCAA AGCAACAGCA AAGTAAAACA 
AAACAAAATG TGTTATCACC TGAAAAAGAG AAGCAGATGT CAGAGCCACT AGATCAAAAA 
AAAATTAGGT CAGATCATAA TGAAGAAGAT GAGAAGGCCT GTGTGCTACA AGTAGTCTGG 
AATGGACTTG AAGAATTGAA CAATGCCATC AAATATACCA AAAATGTAGA AGTTCTCCAT 
CTTGGGAAAG TCTGGATTCC AGATGACCTG AGGAAGAGAC TAAATATAGA AATGCATGCC 
GTAGTCAGGA TAACTCCAGT GGAAGTTACC CCTAAAATTC CAAGATCTCT AAAGTTACAA 
CCTAGAGAGA ATTTACCTAA AGACATAAGT GAAGAAGACA TAAAAACTGT ATTTTATTCA 
TGGCTACAGC AGTCTACTAC CACCATGCTT CCTTTGGTAA TATCAGAGGA AGAATTTATT 
AAGCTGGAAA CTAAAGATGG ACTGAAGGAA TTTTCTCTGA GTATAGTTCA TTCTTGGGAA 
AAAGAAAAAG ATAAAAATAT TTTTCTGTTG AGTCCCAATT TGCTGCAGAA GACTACAATA 
CAAGTCCTTC TAGATCCTAT GGTAAAAGAA GAAAACAGTG AGGAAATTGA CTTTATTCTT 
CCTTTTTTAA AGCTGAGCTC TTTGGGAGGA GTGAATTCCT TAGGCGTATC CTCCTTGGAG 
CACATCACTC ACAGCCTCCT GGGACGCCCT TTGTCTCGGC AGCTGATGTC TCTTGTTGCA 
GGACTTAGGA ATGGAGCTCT TTTACTCACA GGAGGAAAGG GAAGTGGAAA ATCAACTTTA 
GCCAAAGCAA TCTGTAAAGA AGCATTTGAC AAACTGGATG CCCATGTGGA GAGAGTTGAC 
TGTAAAGCTT TACGAGGAAA AAGGCTTGAA AACATACAAA AAACCCTAGA GGTGGCTTTC 
TCAGAGGCAG TGTGGATGCA GCCATCTGTT GTCCTGCTGG ATGACCTTGA CCTCATTGCT 
GGACTGCCTG CTGTCCCGGA ACATGAGCAC AGTCCTGATG CGGTGCAGAG CCAGCGGCTT 
GCTCATGCTT TGAATGATAT GATAAAAGAG TTTATCTCCA TGGGAAGTTT GGTTGCACTG 
ATTGCCACAA GTCAGTCTCA GCAATCTCTA CATCCTTTAC TTGTTTCTGC TCAAGGAGTT 
CACATATTTC AGTGCGTCCA ACACATTCAG CCTCCTAATC AGGAACAAAG ATGTGAAATT 
CTGTGTAATG TAATAAAAAA TAAATTGGAC TGTGATATAA ACAAGTTCAC CGATCTTGAC 
CTGCAGCATG TAGCTAAAGA AACTGGCGGG TTTGTGGCTA GAGATTTTAC AGTACTTGTG 
GATCGAGCCA TACATTCTCG ACTCTCTCGT CAGAGTATAT CCACCAGAGA AAAATTAGTT 
TTAACAACAT TGGACTTCCA AAAGGCTCTC CGCGGATTTC TTCCTGCGTC TTTGCGAAGT 
GTCAACCTGC ATAAACCTAG AGACCTGGGT TGGGACAAGA TTGGTGGGTT ACATGAAGTT 
AGGCAGATAC TCATGGATAC TATCCAGTTA CCTGCCAAGT ATCCAGAATT ATTTGCAAAC 
TTGCCCATAC GACAAAGAAC AGGAATACTG TTGTATGGTC CGCCTGGAAC AGGAAAAACC 
TTACTAGCTG GGGTAATTGC ACGAGAGAGT AGAATGAATT TTATAAGTGT CAAGGGGCCA 
GAGTTACTCA GCAAATACAT TGGAGCAAGT GAACAAGCTG TTCGGGATAT TTTTATTAGA 
GCACAGGCTG CAAAGCCCTG CATTCTTTTC TTTGATGAAT TTGAATCCAT TGCTCCTCGG 
CGGGGTCATG ATAATACAGG AGTTACAGAC CGAGTAGTTA ACCAGTTGCT GACTCAGTTG 
GATGGAGTAG AAGGCTTACA GGGTGTTTAT GTATTGGCTG CTACTAGTCG CCCTGACTTG 
ATTGACCCTG CCCTGCTTAG GCCTGGTCGA CTAGATAAAT GTGTATACTG TCCTCCTCCT 
GATCAGGTGT CACGTCTTGA AATTTTAAAT GTCCTCAGTG ACTCTCTACC TCTGGCAGAT 
GATGTTGACC TTCAGCATGT AGCATCAGTA ACTGACTCCT TTACTGGAGC TGATCTGAAA 
GCTTTACTTT ACAATGCCCA ATTGGAGGCC TTACATGGAA TGCTGCTCTC GAGTGGACTC 
CAGGATGGAA GTTCCAGCTC TGATAGTGAC CTAAGTCTGT CTTCAATGGT CTTTCTTAAC 
CATAGCAGTG GCTCTGACGA TTCAGCTGGA GATGGAGAAT GTGGCTTAGA TCAGTCCCTT 
GTTTCTTTAG AGATGTCCGA GATCCTTCCA GATGAATCAA AATTCAATAT GTACCGGCTC 
TACTTTGGAA GCTCTTATGA ATCAGAACTT GGAAATGGAA CCTCTTCTGA TTTGAGCTCA 
CAATGTCTCT CTGCACCAAG CTCCATGACT CAGGATTTGC CTGGAGTTCC TGGGAAAGAC 
CAGTTGTTTT CACAGCCTCC AGTGTTAAGG ACAGCTTCAC AAGAGGGTTG CCAAGAACTT 
ACACAAGAAC AAAGAGATCA ACTGAGGGCA GATATCAGTA TTATCAAAGG CAGATACCGG 
AGCCAAAGTG GAGAGGACGA ATCCATGAAC CAACCAGGAC CAATCAAAAC CAGACTGGCT 
ATTAGTCAGT CACATTTAAT GACTGCACTT GGTCACACAA GACCATCCAT TAGTGAAGAT 
GACTGGAAGA ATTTTGCTGA GCTATATGAA AGCTTTCAAA ATCCAAAGAG GAGAAAAAAT 
CAAAGTGGAA CAATGTTTCG ACCTGGACAG AAAGTAACTT TAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269607.1
CDS	745..3972
Translation	MMKELQTKQLQSNTVGITESNENESEIPVDSSSVASLWTMIGSIFSFQSEKKQETSWGLTEINAFKNMQSKVVPLDNIFRVCKSQPPSIYNASATSVFHKHCAIHVFPWDQEYFDVEPSFTVTYGKLVKLLSPKQQQSKTKQNVLSPEKEKQMSEPLDQKKIRSDHNEEDEKACVLQVVWNGLEELNNAIKYTKNVEVLHLGKVWIPDDLRKRLNIEMHAVVRITPVEVTPKIPRSLKLQPRENLPKDISEEDIKTVFYSWLQQSTTTMLPLVISEEEFIKLETKDGLKEFSLSIVHSWEKEKDKNIFLLSPNLLQKTTIQVLLDPMVKEENSEEIDFILPFLKLSSLGGVNSLGVSSLEHITHSLLGRPLSRQLMSLVAGLRNGALLLTGGKGSGKSTLAKAICKEAFDKLDAHVERVDCKALRGKRLENIQKTLEVAFSEAVWMQPSVVLLDDLDLIAGLPAVPEHEHSPDAVQSQRLAHALNDMIKEFISMGSLVALIATSQSQQSLHPLLVSAQGVHIFQCVQHIQPPNQEQRCEILCNVIKNKLDCDINKFTDLDLQHVAKETGGFVARDFTVLVDRAIHSRLSRQSISTREKLVLTTLDFQKALRGFLPASLRSVNLHKPRDLGWDKIGGLHEVRQILMDTIQLPAKYPELFANLPIRQRTGILLYGPPGTGKTLLAGVIARESRMNFISVKGPELLSKYIGASEQAVRDIFIRAQAAKPCILFFDEFESIAPRRGHDNTGVTDRVVNQLLTQLDGVEGLQGVYVLAATSRPDLIDPALLRPGRLDKCVYCPPPDQVSRLEILNVLSDSLPLADDVDLQHVASVTDSFTGADLKALLYNAQLEALHGMLLSSGLQDGSSSSDSDLSLSSMVFLNHSSGSDDSAGDGECGLDQSLVSLEMSEILPDESKFNMYRLYFGSSYESELGNGTSSDLSSQCLSAPSSMTQDLPGVPGKDQLFSQPPVLRTASQEGCQELTQEQRDQLRADISIIKGRYRSQSGEDESMNQPGPIKTRLAISQSHLMTALGHTRPSISEDDWKNFAELYESFQNPKRRKNQSGTMFRPGQKVTLA

Target ORF information:

RefSeq Version	NM_001282678.2
Organism	Homo sapiens(human)
Definition	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282678.2

ORF Insert Sequence:

ATGATGAAAG AACTTCAAAC CAAGCAACTT CAGTCAAATA CTGTGGGAAT CACTGAATCT 
AATGAAAACG AGTCAGAGAT TCCAGTTGAC TCATCATCAG TAGCAAGTTT ATGGACTATG 
ATAGGAAGCA TTTTTTCCTT TCAATCTGAG AAGAAACAAG AGACATCTTG GGGTTTAACT 
GAAATCAATG CATTCAAAAA TATGCAGTCA AAGGTTGTTC CTCTAGACAA TATTTTCAGA 
GTATGCAAAT CTCAACCTCC TAGTATATAT AACGCGTCAG CAACCTCTGT TTTTCATAAA 
CACTGTGCCA TTCATGTATT TCCATGGGAC CAGGAATATT TTGATGTAGA GCCCAGCTTT 
ACTGTGACAT ATGGAAAGCT AGTTAAGCTA CTTTCTCCAA AGCAACAGCA AAGTAAAACA 
AAACAAAATG TGTTATCACC TGAAAAAGAG AAGCAGATGT CAGAGCCACT AGATCAAAAA 
AAAATTAGGT CAGATCATAA TGAAGAAGAT GAGAAGGCCT GTGTGCTACA AGTAGTCTGG 
AATGGACTTG AAGAATTGAA CAATGCCATC AAATATACCA AAAATGTAGA AGTTCTCCAT 
CTTGGGAAAG TCTGGATTCC AGATGACCTG AGGAAGAGAC TAAATATAGA AATGCATGCC 
GTAGTCAGGA TAACTCCAGT GGAAGTTACC CCTAAAATTC CAAGATCTCT AAAGTTACAA 
CCTAGAGAGA ATTTACCTAA AGACATAAGT GAAGAAGACA TAAAAACTGT ATTTTATTCA 
TGGCTACAGC AGTCTACTAC CACCATGCTT CCTTTGGTAA TATCAGAGGA AGAATTTATT 
AAGCTGGAAA CTAAAGATGG ACTGAAGGAA TTTTCTCTGA GTATAGTTCA TTCTTGGGAA 
AAAGAAAAAG ATAAAAATAT TTTTCTGTTG AGTCCCAATT TGCTGCAGAA GACTACAATA 
CAAGTCCTTC TAGATCCTAT GGTAAAAGAA GAAAACAGTG AGGAAATTGA CTTTATTCTT 
CCTTTTTTAA AGCTGAGCTC TTTGGGAGGA GTGAATTCCT TAGGCGTATC CTCCTTGGAG 
CACATCACTC ACAGCCTCCT GGGACGCCCT TTGTCTCGGC AGCTGATGTC TCTTGTTGCA 
GGACTTAGGA ATGGAGCTCT TTTACTCACA GGAGGAAAGG GAAGTGGAAA ATCAACTTTA 
GCCAAAGCAA TCTGTAAAGA AGCATTTGAC AAACTGGATG CCCATGTGGA GAGAGTTGAC 
TGTAAAGCTT TACGAGGAAA AAGGCTTGAA AACATACAAA AAACCCTAGA GGTGGCTTTC 
TCAGAGGCAG TGTGGATGCA GCCATCTGTT GTCCTGCTGG ATGACCTTGA CCTCATTGCT 
GGACTGCCTG CTGTCCCGGA ACATGAGCAC AGTCCTGATG CGGTGCAGAG CCAGCGGCTT 
GCTCATGCTT TGAATGATAT GATAAAAGAG TTTATCTCCA TGGGAAGTTT GGTTGCACTG 
ATTGCCACAA GTCAGTCTCA GCAATCTCTA CATCCTTTAC TTGTTTCTGC TCAAGGAGTT 
CACATATTTC AGTGCGTCCA ACACATTCAG CCTCCTAATC AGGAACAAAG ATGTGAAATT 
CTGTGTAATG TAATAAAAAA TAAATTGGAC TGTGATATAA ACAAGTTCAC CGATCTTGAC 
CTGCAGCATG TAGCTAAAGA AACTGGCGGG TTTGTGGCTA GAGATTTTAC AGTACTTGTG 
GATCGAGCCA TACATTCTCG ACTCTCTCGT CAGAGTATAT CCACCAGAGA AAAATTAGTT 
TTAACAACAT TGGACTTCCA AAAGGCTCTC CGCGGATTTC TTCCTGCGTC TTTGCGAAGT 
GTCAACCTGC ATAAACCTAG AGACCTGGGT TGGGACAAGA TTGGTGGGTT ACATGAAGTT 
AGGCAGATAC TCATGGATAC TATCCAGTTA CCTGCCAAGT ATCCAGAATT ATTTGCAAAC 
TTGCCCATAC GACAAAGAAC AGGAATACTG TTGTATGGTC CGCCTGGAAC AGGAAAAACC 
TTACTAGCTG GGGTAATTGC ACGAGAGAGT AGAATGAATT TTATAAGTGT CAAGGGGCCA 
GAGTTACTCA GCAAATACAT TGGAGCAAGT GAACAAGCTG TTCGGGATAT TTTTATTAGA 
GCACAGGCTG CAAAGCCCTG CATTCTTTTC TTTGATGAAT TTGAATCCAT TGCTCCTCGG 
CGGGGTCATG ATAATACAGG AGTTACAGAC CGAGTAGTTA ACCAGTTGCT GACTCAGTTG 
GATGGAGTAG AAGGCTTACA GGGTGTTTAT GTATTGGCTG CTACTAGTCG CCCTGACTTG 
ATTGACCCTG CCCTGCTTAG GCCTGGTCGA CTAGATAAAT GTGTATACTG TCCTCCTCCT 
GATCAGGTGT CACGTCTTGA AATTTTAAAT GTCCTCAGTG ACTCTCTACC TCTGGCAGAT 
GATGTTGACC TTCAGCATGT AGCATCAGTA ACTGACTCCT TTACTGGAGC TGATCTGAAA 
GCTTTACTTT ACAATGCCCA ATTGGAGGCC TTACATGGAA TGCTGCTCTC GAGTGGACTC 
CAGGATGGAA GTTCCAGCTC TGATAGTGAC CTAAGTCTGT CTTCAATGGT CTTTCTTAAC 
CATAGCAGTG GCTCTGACGA TTCAGCTGGA GATGGAGAAT GTGGCTTAGA TCAGTCCCTT 
GTTTCTTTAG AGATGTCCGA GATCCTTCCA GATGAATCAA AATTCAATAT GTACCGGCTC 
TACTTTGGAA GCTCTTATGA ATCAGAACTT GGAAATGGAA CCTCTTCTGA TTTGAGCTCA 
CAATGTCTCT CTGCACCAAG CTCCATGACT CAGGATTTGC CTGGAGTTCC TGGGAAAGAC 
CAGTTGTTTT CACAGCCTCC AGTGTTAAGG ACAGCTTCAC AAGAGGGTTG CCAAGAACTT 
ACACAAGAAC AAAGAGATCA ACTGAGGGCA GATATCAGTA TTATCAAAGG CAGATACCGG 
AGCCAAAGTG GAGAGGACGA ATCCATGAAC CAACCAGGAC CAATCAAAAC CAGACTGGCT 
ATTAGTCAGT CACATTTAAT GACTGCACTT GGTCACACAA GACCATCCAT TAGTGAAGAT 
GACTGGAAGA ATTTTGCTGA GCTATATGAA AGCTTTCAAA ATCCAAAGAG GAGAAAAAAT 
CAAAGTGGAA CAATGTTTCG ACCTGGACAG AAAGTAACTT TAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEX1 ( NM_001282677.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269606.1 Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27457
Clone ID Related Accession (Same CDS sequence)	NM_001282677.1 , NM_001282677.2
Accession Version	NM_001282677.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	peroxisome biogenesis factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA313282.1, AB052090.1, DB461678.1, BC035575.1 and AA490966.1. On Sep 18, 2013 this sequence version replaced XM_005250430.1. Transcript Variant: This variant (2) is missing an in-frame coding exon compared to variant 1. The resulting shorter isoform (2) lacks an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB052090.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGGCA GCGATCGCCT GGCGGGTGCT GGGGGAGGCG GGGCGGCAGT GACTGTGGCC 
TTCACCAACG CTCGCGACTG CTTCCTCCAC CTGCCGCGGC GTCTCGTGGC CCAGCTGCAT 
CTGCTGCAGA ATCAAGCTAT AGAAGTGGTC TGGAGTCACC AGCCTGCATT CTTGAGCTGG 
GTGGAAGGCA GGCATTTTAG TGATCAAGGT GAAAATGTGG CTGAAATTAA CAGACAAGTT 
GGTCAAAAAC TTGGACTCTC AAATGGGGGA CAGGTATTTC TCAAGCCATG TTCCCATGTG 
GTATCTTGTC AACAAGTTGA GGTGGAACCC CTCTCAGCAG ATGATTGGGA GATACTGGAG 
CTGCATGCTG TTTCCCTTGA ACAACATCTT CTAGATCAAA TTCGAATAGT TTTTCCAAAA 
GCCATTTTTC CTGTTTGGGT TGATCAACAA ACGTACATAT TTATCCAAAT TGTTGCACTA 
ATACCAGCTG CCTCTTATGG AAGGCTGGAA ACTGACACCA AACTCCTTAT TCAGCCAAAG 
ACACGCCGAG CCAAAGAGAA TACATTTTCA AAAGCTGATG CTGAATATAA AAAACTTCAT 
AGTTATGGAA GAGACCAGAA AGGAATGATG AAAGAACTTC AAACCAAGCA ACTTCAGTCA 
AATACTGTGG GAATCACTGA ATCTAATGAA AACGAGTCAG AGATTCCAGT TGACTCATCA 
TCAGTAGCAA GTTTATGGAC TATGATAGGA AGCATTTTTT CCTTTCAATC TGAGAAGAAA 
CAAGAGACAT CTTGGGGTTT AACTGAAATC AATGCATTCA AAAATATGCA GTCAAAGGTT 
GTTCCTCTAG ACAATATTTT CAGAGTATGC AAATCTCAAC CTCCTAGTAT ATATAACGCG 
TCAGCAACCT CTGTTTTTCA TAAACACTGT GCCATTCATG TATTTCCATG GGACCAGGAA 
TATTTTGATG TAGAGCCCAG CTTTACTGTG ACATATGGAA AGCTAGTTAA GCTACTTTCT 
CCAAAGCAAC AGCAAAGTAA AACAAAACAA AATGTGTTAT CACCTGAAAA AGAGAAGCAG 
ATGTCAGAGC CACTAGATCA AAAAAAAATT AGGTCAGATC ATAATGAAGA AGATGAGAAG 
GCCTGTGTGC TACAAGTAGT CTGGAATGGA CTTGAAGAAT TGAACAATGC CATCAAATAT 
ACCAAAAATG TAGAAGTTCT CCATCTTGGG AAAGTCTGGA TTCCAGATGA CCTGAGGAAG 
AGACTAAATA TAGAAATGCA TGCCGTAGTC AGGATAACTC CAGTGGAAGT TACCCCTAAA 
ATTCCAAGAT CTCTAAAGTT ACAACCTAGA GAGAATTTAC CTAAAGACAT AAGTGAAGAA 
GACATAAAAA CTGTATTTTA TTCATGGCTA CAGCAGTCTA CTACCACCAT GCTTCCTTTG 
GTAATATCAG AGGAAGAATT TATTAAGCTG GAAACTAAAG ATGGACTGAA GGAATTTTCT 
CTGAGTATAG TTCATTCTTG GGAAAAAGAA AAAGATAAAA ATATTTTTCT GTTGAGTCCC 
AATTTGCTGC AGAAGACTAC AATACAAGTC CTTCTAGATC CTATGGTAAA AGAAGAAAAC 
AGTGAGGAAA TTGACTTTAT TCTTCCTTTT TTAAAGCTGA GCTCTTTGGG AGGAGTGAAT 
TCCTTAGGCG TATCCTCCTT GGAGCACATC ACTCACAGCC TCCTGGGACG CCCTTTGTCT 
CGGCAGCTGA TGTCTCTTGT TGCAGGACTT AGGAATGGAG CTCTTTTACT CACAGGAGGA 
AAGGGAAGTG GAAAATCAAC TTTAGCCAAA GCAATCTGTA AAGAAGCATT TGACAAACTG 
GATGCCCATG TGGAGAGAGT TGACTGTAAA GCTTTACGAG CTTTGAATGA TATGATAAAA 
GAGTTTATCT CCATGGGAAG TTTGGTTGCA CTGATTGCCA CAAGTCAGTC TCAGCAATCT 
CTACATCCTT TACTTGTTTC TGCTCAAGGA GTTCACATAT TTCAGTGCGT CCAACACATT 
CAGCCTCCTA ATCAGGAACA AAGATGTGAA ATTCTGTGTA ATGTAATAAA AAATAAATTG 
GACTGTGATA TAAACAAGTT CACCGATCTT GACCTGCAGC ATGTAGCTAA AGAAACTGGC 
GGGTTTGTGG CTAGAGATTT TACAGTACTT GTGGATCGAG CCATACATTC TCGACTCTCT 
CGTCAGAGTA TATCCACCAG AGAAAAATTA GTTTTAACAA CATTGGACTT CCAAAAGGCT 
CTCCGCGGAT TTCTTCCTGC GTCTTTGCGA AGTGTCAACC TGCATAAACC TAGAGACCTG 
GGTTGGGACA AGATTGGTGG GTTACATGAA GTTAGGCAGA TACTCATGGA TACTATCCAG 
TTACCTGCCA AGTATCCAGA ATTATTTGCA AACTTGCCCA TACGACAAAG AACAGGAATA 
CTGTTGTATG GTCCGCCTGG AACAGGAAAA ACCTTACTAG CTGGGGTAAT TGCACGAGAG 
AGTAGAATGA ATTTTATAAG TGTCAAGGGG CCAGAGTTAC TCAGCAAATA CATTGGAGCA 
AGTGAACAAG CTGTTCGGGA TATTTTTATT AGAGCACAGG CTGCAAAGCC CTGCATTCTT 
TTCTTTGATG AATTTGAATC CATTGCTCCT CGGCGGGGTC ATGATAATAC AGGAGTTACA 
GACCGAGTAG TTAACCAGTT GCTGACTCAG TTGGATGGAG TAGAAGGCTT ACAGGGTGTT 
TATGTATTGG CTGCTACTAG TCGCCCTGAC TTGATTGACC CTGCCCTGCT TAGGCCTGGT 
CGACTAGATA AATGTGTATA CTGTCCTCCT CCTGATCAGG TGTCACGTCT TGAAATTTTA 
AATGTCCTCA GTGACTCTCT ACCTCTGGCA GATGATGTTG ACCTTCAGCA TGTAGCATCA 
GTAACTGACT CCTTTACTGG AGCTGATCTG AAAGCTTTAC TTTACAATGC CCAATTGGAG 
GCCTTACATG GAATGCTGCT CTCGAGTGGA CTCCAGGATG GAAGTTCCAG CTCTGATAGT 
GACCTAAGTC TGTCTTCAAT GGTCTTTCTT AACCATAGCA GTGGCTCTGA CGATTCAGCT 
GGAGATGGAG AATGTGGCTT AGATCAGTCC CTTGTTTCTT TAGAGATGTC CGAGATCCTT 
CCAGATGAAT CAAAATTCAA TATGTACCGG CTCTACTTTG GAAGCTCTTA TGAATCAGAA 
CTTGGAAATG GAACCTCTTC TGATTTGAGC TCACAATGTC TCTCTGCACC AAGCTCCATG 
ACTCAGGATT TGCCTGGAGT TCCTGGGAAA GACCAGTTGT TTTCACAGCC TCCAGTGTTA 
AGGACAGCTT CACAAGAGGG TTGCCAAGAA CTTACACAAG AACAAAGAGA TCAACTGAGG 
GCAGATATCA GTATTATCAA AGGCAGATAC CGGAGCCAAA GTGGAGAGGA CGAATCCATG 
AACCAACCAG GACCAATCAA AACCAGACTG GCTATTAGTC AGTCACATTT AATGACTGCA 
CTTGGTCACA CAAGACCATC CATTAGTGAA GATGACTGGA AGAATTTTGC TGAGCTATAT 
GAAAGCTTTC AAAATCCAAA GAGGAGAAAA AATCAAAGTG GAACAATGTT TCGACCTGGA 
CAGAAAGTAA CTTTAGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269606.1
CDS	97..3777
Translation	MWGSDRLAGAGGGGAAVTVAFTNARDCFLHLPRRLVAQLHLLQNQAIEVVWSHQPAFLSWVEGRHFSDQGENVAEINRQVGQKLGLSNGGQVFLKPCSHVVSCQQVEVEPLSADDWEILELHAVSLEQHLLDQIRIVFPKAIFPVWVDQQTYIFIQIVALIPAASYGRLETDTKLLIQPKTRRAKENTFSKADAEYKKLHSYGRDQKGMMKELQTKQLQSNTVGITESNENESEIPVDSSSVASLWTMIGSIFSFQSEKKQETSWGLTEINAFKNMQSKVVPLDNIFRVCKSQPPSIYNASATSVFHKHCAIHVFPWDQEYFDVEPSFTVTYGKLVKLLSPKQQQSKTKQNVLSPEKEKQMSEPLDQKKIRSDHNEEDEKACVLQVVWNGLEELNNAIKYTKNVEVLHLGKVWIPDDLRKRLNIEMHAVVRITPVEVTPKIPRSLKLQPRENLPKDISEEDIKTVFYSWLQQSTTTMLPLVISEEEFIKLETKDGLKEFSLSIVHSWEKEKDKNIFLLSPNLLQKTTIQVLLDPMVKEENSEEIDFILPFLKLSSLGGVNSLGVSSLEHITHSLLGRPLSRQLMSLVAGLRNGALLLTGGKGSGKSTLAKAICKEAFDKLDAHVERVDCKALRALNDMIKEFISMGSLVALIATSQSQQSLHPLLVSAQGVHIFQCVQHIQPPNQEQRCEILCNVIKNKLDCDINKFTDLDLQHVAKETGGFVARDFTVLVDRAIHSRLSRQSISTREKLVLTTLDFQKALRGFLPASLRSVNLHKPRDLGWDKIGGLHEVRQILMDTIQLPAKYPELFANLPIRQRTGILLYGPPGTGKTLLAGVIARESRMNFISVKGPELLSKYIGASEQAVRDIFIRAQAAKPCILFFDEFESIAPRRGHDNTGVTDRVVNQLLTQLDGVEGLQGVYVLAATSRPDLIDPALLRPGRLDKCVYCPPPDQVSRLEILNVLSDSLPLADDVDLQHVASVTDSFTGADLKALLYNAQLEALHGMLLSSGLQDGSSSSDSDLSLSSMVFLNHSSGSDDSAGDGECGLDQSLVSLEMSEILPDESKFNMYRLYFGSSYESELGNGTSSDLSSQCLSAPSSMTQDLPGVPGKDQLFSQPPVLRTASQEGCQELTQEQRDQLRADISIIKGRYRSQSGEDESMNQPGPIKTRLAISQSHLMTALGHTRPSISEDDWKNFAELYESFQNPKRRKNQSGTMFRPGQKVTLA

Target ORF information:

RefSeq Version	NM_001282677.1
Organism	Homo sapiens(human)
Definition	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282677.1

ORF Insert Sequence:

ATGTGGGGCA GCGATCGCCT GGCGGGTGCT GGGGGAGGCG GGGCGGCAGT GACTGTGGCC 
TTCACCAACG CTCGCGACTG CTTCCTCCAC CTGCCGCGGC GTCTCGTGGC CCAGCTGCAT 
CTGCTGCAGA ATCAAGCTAT AGAAGTGGTC TGGAGTCACC AGCCTGCATT CTTGAGCTGG 
GTGGAAGGCA GGCATTTTAG TGATCAAGGT GAAAATGTGG CTGAAATTAA CAGACAAGTT 
GGTCAAAAAC TTGGACTCTC AAATGGGGGA CAGGTATTTC TCAAGCCATG TTCCCATGTG 
GTATCTTGTC AACAAGTTGA GGTGGAACCC CTCTCAGCAG ATGATTGGGA GATACTGGAG 
CTGCATGCTG TTTCCCTTGA ACAACATCTT CTAGATCAAA TTCGAATAGT TTTTCCAAAA 
GCCATTTTTC CTGTTTGGGT TGATCAACAA ACGTACATAT TTATCCAAAT TGTTGCACTA 
ATACCAGCTG CCTCTTATGG AAGGCTGGAA ACTGACACCA AACTCCTTAT TCAGCCAAAG 
ACACGCCGAG CCAAAGAGAA TACATTTTCA AAAGCTGATG CTGAATATAA AAAACTTCAT 
AGTTATGGAA GAGACCAGAA AGGAATGATG AAAGAACTTC AAACCAAGCA ACTTCAGTCA 
AATACTGTGG GAATCACTGA ATCTAATGAA AACGAGTCAG AGATTCCAGT TGACTCATCA 
TCAGTAGCAA GTTTATGGAC TATGATAGGA AGCATTTTTT CCTTTCAATC TGAGAAGAAA 
CAAGAGACAT CTTGGGGTTT AACTGAAATC AATGCATTCA AAAATATGCA GTCAAAGGTT 
GTTCCTCTAG ACAATATTTT CAGAGTATGC AAATCTCAAC CTCCTAGTAT ATATAACGCG 
TCAGCAACCT CTGTTTTTCA TAAACACTGT GCCATTCATG TATTTCCATG GGACCAGGAA 
TATTTTGATG TAGAGCCCAG CTTTACTGTG ACATATGGAA AGCTAGTTAA GCTACTTTCT 
CCAAAGCAAC AGCAAAGTAA AACAAAACAA AATGTGTTAT CACCTGAAAA AGAGAAGCAG 
ATGTCAGAGC CACTAGATCA AAAAAAAATT AGGTCAGATC ATAATGAAGA AGATGAGAAG 
GCCTGTGTGC TACAAGTAGT CTGGAATGGA CTTGAAGAAT TGAACAATGC CATCAAATAT 
ACCAAAAATG TAGAAGTTCT CCATCTTGGG AAAGTCTGGA TTCCAGATGA CCTGAGGAAG 
AGACTAAATA TAGAAATGCA TGCCGTAGTC AGGATAACTC CAGTGGAAGT TACCCCTAAA 
ATTCCAAGAT CTCTAAAGTT ACAACCTAGA GAGAATTTAC CTAAAGACAT AAGTGAAGAA 
GACATAAAAA CTGTATTTTA TTCATGGCTA CAGCAGTCTA CTACCACCAT GCTTCCTTTG 
GTAATATCAG AGGAAGAATT TATTAAGCTG GAAACTAAAG ATGGACTGAA GGAATTTTCT 
CTGAGTATAG TTCATTCTTG GGAAAAAGAA AAAGATAAAA ATATTTTTCT GTTGAGTCCC 
AATTTGCTGC AGAAGACTAC AATACAAGTC CTTCTAGATC CTATGGTAAA AGAAGAAAAC 
AGTGAGGAAA TTGACTTTAT TCTTCCTTTT TTAAAGCTGA GCTCTTTGGG AGGAGTGAAT 
TCCTTAGGCG TATCCTCCTT GGAGCACATC ACTCACAGCC TCCTGGGACG CCCTTTGTCT 
CGGCAGCTGA TGTCTCTTGT TGCAGGACTT AGGAATGGAG CTCTTTTACT CACAGGAGGA 
AAGGGAAGTG GAAAATCAAC TTTAGCCAAA GCAATCTGTA AAGAAGCATT TGACAAACTG 
GATGCCCATG TGGAGAGAGT TGACTGTAAA GCTTTACGAG CTTTGAATGA TATGATAAAA 
GAGTTTATCT CCATGGGAAG TTTGGTTGCA CTGATTGCCA CAAGTCAGTC TCAGCAATCT 
CTACATCCTT TACTTGTTTC TGCTCAAGGA GTTCACATAT TTCAGTGCGT CCAACACATT 
CAGCCTCCTA ATCAGGAACA AAGATGTGAA ATTCTGTGTA ATGTAATAAA AAATAAATTG 
GACTGTGATA TAAACAAGTT CACCGATCTT GACCTGCAGC ATGTAGCTAA AGAAACTGGC 
GGGTTTGTGG CTAGAGATTT TACAGTACTT GTGGATCGAG CCATACATTC TCGACTCTCT 
CGTCAGAGTA TATCCACCAG AGAAAAATTA GTTTTAACAA CATTGGACTT CCAAAAGGCT 
CTCCGCGGAT TTCTTCCTGC GTCTTTGCGA AGTGTCAACC TGCATAAACC TAGAGACCTG 
GGTTGGGACA AGATTGGTGG GTTACATGAA GTTAGGCAGA TACTCATGGA TACTATCCAG 
TTACCTGCCA AGTATCCAGA ATTATTTGCA AACTTGCCCA TACGACAAAG AACAGGAATA 
CTGTTGTATG GTCCGCCTGG AACAGGAAAA ACCTTACTAG CTGGGGTAAT TGCACGAGAG 
AGTAGAATGA ATTTTATAAG TGTCAAGGGG CCAGAGTTAC TCAGCAAATA CATTGGAGCA 
AGTGAACAAG CTGTTCGGGA TATTTTTATT AGAGCACAGG CTGCAAAGCC CTGCATTCTT 
TTCTTTGATG AATTTGAATC CATTGCTCCT CGGCGGGGTC ATGATAATAC AGGAGTTACA 
GACCGAGTAG TTAACCAGTT GCTGACTCAG TTGGATGGAG TAGAAGGCTT ACAGGGTGTT 
TATGTATTGG CTGCTACTAG TCGCCCTGAC TTGATTGACC CTGCCCTGCT TAGGCCTGGT 
CGACTAGATA AATGTGTATA CTGTCCTCCT CCTGATCAGG TGTCACGTCT TGAAATTTTA 
AATGTCCTCA GTGACTCTCT ACCTCTGGCA GATGATGTTG ACCTTCAGCA TGTAGCATCA 
GTAACTGACT CCTTTACTGG AGCTGATCTG AAAGCTTTAC TTTACAATGC CCAATTGGAG 
GCCTTACATG GAATGCTGCT CTCGAGTGGA CTCCAGGATG GAAGTTCCAG CTCTGATAGT 
GACCTAAGTC TGTCTTCAAT GGTCTTTCTT AACCATAGCA GTGGCTCTGA CGATTCAGCT 
GGAGATGGAG AATGTGGCTT AGATCAGTCC CTTGTTTCTT TAGAGATGTC CGAGATCCTT 
CCAGATGAAT CAAAATTCAA TATGTACCGG CTCTACTTTG GAAGCTCTTA TGAATCAGAA 
CTTGGAAATG GAACCTCTTC TGATTTGAGC TCACAATGTC TCTCTGCACC AAGCTCCATG 
ACTCAGGATT TGCCTGGAGT TCCTGGGAAA GACCAGTTGT TTTCACAGCC TCCAGTGTTA 
AGGACAGCTT CACAAGAGGG TTGCCAAGAA CTTACACAAG AACAAAGAGA TCAACTGAGG 
GCAGATATCA GTATTATCAA AGGCAGATAC CGGAGCCAAA GTGGAGAGGA CGAATCCATG 
AACCAACCAG GACCAATCAA AACCAGACTG GCTATTAGTC AGTCACATTT AATGACTGCA 
CTTGGTCACA CAAGACCATC CATTAGTGAA GATGACTGGA AGAATTTTGC TGAGCTATAT 
GAAAGCTTTC AAAATCCAAA GAGGAGAAAA AATCAAAGTG GAACAATGTT TCGACCTGGA 
CAGAAAGTAA CTTTAGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PEX1 ( NM_001282677.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269606.1 Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 2, mRNA.

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CloneID	OHu27457
Clone ID Related Accession (Same CDS sequence)	NM_001282677.1 , NM_001282677.2
Accession Version	NM_001282677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	peroxisome biogenesis factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA313282.1, AB052090.1, DB461678.1 and BC035575.1. On May 31, 2019 this sequence version replaced NM_001282677.1. Transcript Variant: This variant (2) is missing an in-frame coding exon compared to variant 1. The resulting shorter isoform (2) lacks an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB052090.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGGCA GCGATCGCCT GGCGGGTGCT GGGGGAGGCG GGGCGGCAGT GACTGTGGCC 
TTCACCAACG CTCGCGACTG CTTCCTCCAC CTGCCGCGGC GTCTCGTGGC CCAGCTGCAT 
CTGCTGCAGA ATCAAGCTAT AGAAGTGGTC TGGAGTCACC AGCCTGCATT CTTGAGCTGG 
GTGGAAGGCA GGCATTTTAG TGATCAAGGT GAAAATGTGG CTGAAATTAA CAGACAAGTT 
GGTCAAAAAC TTGGACTCTC AAATGGGGGA CAGGTATTTC TCAAGCCATG TTCCCATGTG 
GTATCTTGTC AACAAGTTGA GGTGGAACCC CTCTCAGCAG ATGATTGGGA GATACTGGAG 
CTGCATGCTG TTTCCCTTGA ACAACATCTT CTAGATCAAA TTCGAATAGT TTTTCCAAAA 
GCCATTTTTC CTGTTTGGGT TGATCAACAA ACGTACATAT TTATCCAAAT TGTTGCACTA 
ATACCAGCTG CCTCTTATGG AAGGCTGGAA ACTGACACCA AACTCCTTAT TCAGCCAAAG 
ACACGCCGAG CCAAAGAGAA TACATTTTCA AAAGCTGATG CTGAATATAA AAAACTTCAT 
AGTTATGGAA GAGACCAGAA AGGAATGATG AAAGAACTTC AAACCAAGCA ACTTCAGTCA 
AATACTGTGG GAATCACTGA ATCTAATGAA AACGAGTCAG AGATTCCAGT TGACTCATCA 
TCAGTAGCAA GTTTATGGAC TATGATAGGA AGCATTTTTT CCTTTCAATC TGAGAAGAAA 
CAAGAGACAT CTTGGGGTTT AACTGAAATC AATGCATTCA AAAATATGCA GTCAAAGGTT 
GTTCCTCTAG ACAATATTTT CAGAGTATGC AAATCTCAAC CTCCTAGTAT ATATAACGCG 
TCAGCAACCT CTGTTTTTCA TAAACACTGT GCCATTCATG TATTTCCATG GGACCAGGAA 
TATTTTGATG TAGAGCCCAG CTTTACTGTG ACATATGGAA AGCTAGTTAA GCTACTTTCT 
CCAAAGCAAC AGCAAAGTAA AACAAAACAA AATGTGTTAT CACCTGAAAA AGAGAAGCAG 
ATGTCAGAGC CACTAGATCA AAAAAAAATT AGGTCAGATC ATAATGAAGA AGATGAGAAG 
GCCTGTGTGC TACAAGTAGT CTGGAATGGA CTTGAAGAAT TGAACAATGC CATCAAATAT 
ACCAAAAATG TAGAAGTTCT CCATCTTGGG AAAGTCTGGA TTCCAGATGA CCTGAGGAAG 
AGACTAAATA TAGAAATGCA TGCCGTAGTC AGGATAACTC CAGTGGAAGT TACCCCTAAA 
ATTCCAAGAT CTCTAAAGTT ACAACCTAGA GAGAATTTAC CTAAAGACAT AAGTGAAGAA 
GACATAAAAA CTGTATTTTA TTCATGGCTA CAGCAGTCTA CTACCACCAT GCTTCCTTTG 
GTAATATCAG AGGAAGAATT TATTAAGCTG GAAACTAAAG ATGGACTGAA GGAATTTTCT 
CTGAGTATAG TTCATTCTTG GGAAAAAGAA AAAGATAAAA ATATTTTTCT GTTGAGTCCC 
AATTTGCTGC AGAAGACTAC AATACAAGTC CTTCTAGATC CTATGGTAAA AGAAGAAAAC 
AGTGAGGAAA TTGACTTTAT TCTTCCTTTT TTAAAGCTGA GCTCTTTGGG AGGAGTGAAT 
TCCTTAGGCG TATCCTCCTT GGAGCACATC ACTCACAGCC TCCTGGGACG CCCTTTGTCT 
CGGCAGCTGA TGTCTCTTGT TGCAGGACTT AGGAATGGAG CTCTTTTACT CACAGGAGGA 
AAGGGAAGTG GAAAATCAAC TTTAGCCAAA GCAATCTGTA AAGAAGCATT TGACAAACTG 
GATGCCCATG TGGAGAGAGT TGACTGTAAA GCTTTACGAG CTTTGAATGA TATGATAAAA 
GAGTTTATCT CCATGGGAAG TTTGGTTGCA CTGATTGCCA CAAGTCAGTC TCAGCAATCT 
CTACATCCTT TACTTGTTTC TGCTCAAGGA GTTCACATAT TTCAGTGCGT CCAACACATT 
CAGCCTCCTA ATCAGGAACA AAGATGTGAA ATTCTGTGTA ATGTAATAAA AAATAAATTG 
GACTGTGATA TAAACAAGTT CACCGATCTT GACCTGCAGC ATGTAGCTAA AGAAACTGGC 
GGGTTTGTGG CTAGAGATTT TACAGTACTT GTGGATCGAG CCATACATTC TCGACTCTCT 
CGTCAGAGTA TATCCACCAG AGAAAAATTA GTTTTAACAA CATTGGACTT CCAAAAGGCT 
CTCCGCGGAT TTCTTCCTGC GTCTTTGCGA AGTGTCAACC TGCATAAACC TAGAGACCTG 
GGTTGGGACA AGATTGGTGG GTTACATGAA GTTAGGCAGA TACTCATGGA TACTATCCAG 
TTACCTGCCA AGTATCCAGA ATTATTTGCA AACTTGCCCA TACGACAAAG AACAGGAATA 
CTGTTGTATG GTCCGCCTGG AACAGGAAAA ACCTTACTAG CTGGGGTAAT TGCACGAGAG 
AGTAGAATGA ATTTTATAAG TGTCAAGGGG CCAGAGTTAC TCAGCAAATA CATTGGAGCA 
AGTGAACAAG CTGTTCGGGA TATTTTTATT AGAGCACAGG CTGCAAAGCC CTGCATTCTT 
TTCTTTGATG AATTTGAATC CATTGCTCCT CGGCGGGGTC ATGATAATAC AGGAGTTACA 
GACCGAGTAG TTAACCAGTT GCTGACTCAG TTGGATGGAG TAGAAGGCTT ACAGGGTGTT 
TATGTATTGG CTGCTACTAG TCGCCCTGAC TTGATTGACC CTGCCCTGCT TAGGCCTGGT 
CGACTAGATA AATGTGTATA CTGTCCTCCT CCTGATCAGG TGTCACGTCT TGAAATTTTA 
AATGTCCTCA GTGACTCTCT ACCTCTGGCA GATGATGTTG ACCTTCAGCA TGTAGCATCA 
GTAACTGACT CCTTTACTGG AGCTGATCTG AAAGCTTTAC TTTACAATGC CCAATTGGAG 
GCCTTACATG GAATGCTGCT CTCGAGTGGA CTCCAGGATG GAAGTTCCAG CTCTGATAGT 
GACCTAAGTC TGTCTTCAAT GGTCTTTCTT AACCATAGCA GTGGCTCTGA CGATTCAGCT 
GGAGATGGAG AATGTGGCTT AGATCAGTCC CTTGTTTCTT TAGAGATGTC CGAGATCCTT 
CCAGATGAAT CAAAATTCAA TATGTACCGG CTCTACTTTG GAAGCTCTTA TGAATCAGAA 
CTTGGAAATG GAACCTCTTC TGATTTGAGC TCACAATGTC TCTCTGCACC AAGCTCCATG 
ACTCAGGATT TGCCTGGAGT TCCTGGGAAA GACCAGTTGT TTTCACAGCC TCCAGTGTTA 
AGGACAGCTT CACAAGAGGG TTGCCAAGAA CTTACACAAG AACAAAGAGA TCAACTGAGG 
GCAGATATCA GTATTATCAA AGGCAGATAC CGGAGCCAAA GTGGAGAGGA CGAATCCATG 
AACCAACCAG GACCAATCAA AACCAGACTG GCTATTAGTC AGTCACATTT AATGACTGCA 
CTTGGTCACA CAAGACCATC CATTAGTGAA GATGACTGGA AGAATTTTGC TGAGCTATAT 
GAAAGCTTTC AAAATCCAAA GAGGAGAAAA AATCAAAGTG GAACAATGTT TCGACCTGGA 
CAGAAAGTAA CTTTAGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269606.1
CDS	86..3766
Translation	MWGSDRLAGAGGGGAAVTVAFTNARDCFLHLPRRLVAQLHLLQNQAIEVVWSHQPAFLSWVEGRHFSDQGENVAEINRQVGQKLGLSNGGQVFLKPCSHVVSCQQVEVEPLSADDWEILELHAVSLEQHLLDQIRIVFPKAIFPVWVDQQTYIFIQIVALIPAASYGRLETDTKLLIQPKTRRAKENTFSKADAEYKKLHSYGRDQKGMMKELQTKQLQSNTVGITESNENESEIPVDSSSVASLWTMIGSIFSFQSEKKQETSWGLTEINAFKNMQSKVVPLDNIFRVCKSQPPSIYNASATSVFHKHCAIHVFPWDQEYFDVEPSFTVTYGKLVKLLSPKQQQSKTKQNVLSPEKEKQMSEPLDQKKIRSDHNEEDEKACVLQVVWNGLEELNNAIKYTKNVEVLHLGKVWIPDDLRKRLNIEMHAVVRITPVEVTPKIPRSLKLQPRENLPKDISEEDIKTVFYSWLQQSTTTMLPLVISEEEFIKLETKDGLKEFSLSIVHSWEKEKDKNIFLLSPNLLQKTTIQVLLDPMVKEENSEEIDFILPFLKLSSLGGVNSLGVSSLEHITHSLLGRPLSRQLMSLVAGLRNGALLLTGGKGSGKSTLAKAICKEAFDKLDAHVERVDCKALRALNDMIKEFISMGSLVALIATSQSQQSLHPLLVSAQGVHIFQCVQHIQPPNQEQRCEILCNVIKNKLDCDINKFTDLDLQHVAKETGGFVARDFTVLVDRAIHSRLSRQSISTREKLVLTTLDFQKALRGFLPASLRSVNLHKPRDLGWDKIGGLHEVRQILMDTIQLPAKYPELFANLPIRQRTGILLYGPPGTGKTLLAGVIARESRMNFISVKGPELLSKYIGASEQAVRDIFIRAQAAKPCILFFDEFESIAPRRGHDNTGVTDRVVNQLLTQLDGVEGLQGVYVLAATSRPDLIDPALLRPGRLDKCVYCPPPDQVSRLEILNVLSDSLPLADDVDLQHVASVTDSFTGADLKALLYNAQLEALHGMLLSSGLQDGSSSSDSDLSLSSMVFLNHSSGSDDSAGDGECGLDQSLVSLEMSEILPDESKFNMYRLYFGSSYESELGNGTSSDLSSQCLSAPSSMTQDLPGVPGKDQLFSQPPVLRTASQEGCQELTQEQRDQLRADISIIKGRYRSQSGEDESMNQPGPIKTRLAISQSHLMTALGHTRPSISEDDWKNFAELYESFQNPKRRKNQSGTMFRPGQKVTLA

Target ORF information:

RefSeq Version	NM_001282677.2
Organism	Homo sapiens(human)
Definition	Homo sapiens peroxisomal biogenesis factor 1 (PEX1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282677.2

ORF Insert Sequence:

ATGTGGGGCA GCGATCGCCT GGCGGGTGCT GGGGGAGGCG GGGCGGCAGT GACTGTGGCC 
TTCACCAACG CTCGCGACTG CTTCCTCCAC CTGCCGCGGC GTCTCGTGGC CCAGCTGCAT 
CTGCTGCAGA ATCAAGCTAT AGAAGTGGTC TGGAGTCACC AGCCTGCATT CTTGAGCTGG 
GTGGAAGGCA GGCATTTTAG TGATCAAGGT GAAAATGTGG CTGAAATTAA CAGACAAGTT 
GGTCAAAAAC TTGGACTCTC AAATGGGGGA CAGGTATTTC TCAAGCCATG TTCCCATGTG 
GTATCTTGTC AACAAGTTGA GGTGGAACCC CTCTCAGCAG ATGATTGGGA GATACTGGAG 
CTGCATGCTG TTTCCCTTGA ACAACATCTT CTAGATCAAA TTCGAATAGT TTTTCCAAAA 
GCCATTTTTC CTGTTTGGGT TGATCAACAA ACGTACATAT TTATCCAAAT TGTTGCACTA 
ATACCAGCTG CCTCTTATGG AAGGCTGGAA ACTGACACCA AACTCCTTAT TCAGCCAAAG 
ACACGCCGAG CCAAAGAGAA TACATTTTCA AAAGCTGATG CTGAATATAA AAAACTTCAT 
AGTTATGGAA GAGACCAGAA AGGAATGATG AAAGAACTTC AAACCAAGCA ACTTCAGTCA 
AATACTGTGG GAATCACTGA ATCTAATGAA AACGAGTCAG AGATTCCAGT TGACTCATCA 
TCAGTAGCAA GTTTATGGAC TATGATAGGA AGCATTTTTT CCTTTCAATC TGAGAAGAAA 
CAAGAGACAT CTTGGGGTTT AACTGAAATC AATGCATTCA AAAATATGCA GTCAAAGGTT 
GTTCCTCTAG ACAATATTTT CAGAGTATGC AAATCTCAAC CTCCTAGTAT ATATAACGCG 
TCAGCAACCT CTGTTTTTCA TAAACACTGT GCCATTCATG TATTTCCATG GGACCAGGAA 
TATTTTGATG TAGAGCCCAG CTTTACTGTG ACATATGGAA AGCTAGTTAA GCTACTTTCT 
CCAAAGCAAC AGCAAAGTAA AACAAAACAA AATGTGTTAT CACCTGAAAA AGAGAAGCAG 
ATGTCAGAGC CACTAGATCA AAAAAAAATT AGGTCAGATC ATAATGAAGA AGATGAGAAG 
GCCTGTGTGC TACAAGTAGT CTGGAATGGA CTTGAAGAAT TGAACAATGC CATCAAATAT 
ACCAAAAATG TAGAAGTTCT CCATCTTGGG AAAGTCTGGA TTCCAGATGA CCTGAGGAAG 
AGACTAAATA TAGAAATGCA TGCCGTAGTC AGGATAACTC CAGTGGAAGT TACCCCTAAA 
ATTCCAAGAT CTCTAAAGTT ACAACCTAGA GAGAATTTAC CTAAAGACAT AAGTGAAGAA 
GACATAAAAA CTGTATTTTA TTCATGGCTA CAGCAGTCTA CTACCACCAT GCTTCCTTTG 
GTAATATCAG AGGAAGAATT TATTAAGCTG GAAACTAAAG ATGGACTGAA GGAATTTTCT 
CTGAGTATAG TTCATTCTTG GGAAAAAGAA AAAGATAAAA ATATTTTTCT GTTGAGTCCC 
AATTTGCTGC AGAAGACTAC AATACAAGTC CTTCTAGATC CTATGGTAAA AGAAGAAAAC 
AGTGAGGAAA TTGACTTTAT TCTTCCTTTT TTAAAGCTGA GCTCTTTGGG AGGAGTGAAT 
TCCTTAGGCG TATCCTCCTT GGAGCACATC ACTCACAGCC TCCTGGGACG CCCTTTGTCT 
CGGCAGCTGA TGTCTCTTGT TGCAGGACTT AGGAATGGAG CTCTTTTACT CACAGGAGGA 
AAGGGAAGTG GAAAATCAAC TTTAGCCAAA GCAATCTGTA AAGAAGCATT TGACAAACTG 
GATGCCCATG TGGAGAGAGT TGACTGTAAA GCTTTACGAG CTTTGAATGA TATGATAAAA 
GAGTTTATCT CCATGGGAAG TTTGGTTGCA CTGATTGCCA CAAGTCAGTC TCAGCAATCT 
CTACATCCTT TACTTGTTTC TGCTCAAGGA GTTCACATAT TTCAGTGCGT CCAACACATT 
CAGCCTCCTA ATCAGGAACA AAGATGTGAA ATTCTGTGTA ATGTAATAAA AAATAAATTG 
GACTGTGATA TAAACAAGTT CACCGATCTT GACCTGCAGC ATGTAGCTAA AGAAACTGGC 
GGGTTTGTGG CTAGAGATTT TACAGTACTT GTGGATCGAG CCATACATTC TCGACTCTCT 
CGTCAGAGTA TATCCACCAG AGAAAAATTA GTTTTAACAA CATTGGACTT CCAAAAGGCT 
CTCCGCGGAT TTCTTCCTGC GTCTTTGCGA AGTGTCAACC TGCATAAACC TAGAGACCTG 
GGTTGGGACA AGATTGGTGG GTTACATGAA GTTAGGCAGA TACTCATGGA TACTATCCAG 
TTACCTGCCA AGTATCCAGA ATTATTTGCA AACTTGCCCA TACGACAAAG AACAGGAATA 
CTGTTGTATG GTCCGCCTGG AACAGGAAAA ACCTTACTAG CTGGGGTAAT TGCACGAGAG 
AGTAGAATGA ATTTTATAAG TGTCAAGGGG CCAGAGTTAC TCAGCAAATA CATTGGAGCA 
AGTGAACAAG CTGTTCGGGA TATTTTTATT AGAGCACAGG CTGCAAAGCC CTGCATTCTT 
TTCTTTGATG AATTTGAATC CATTGCTCCT CGGCGGGGTC ATGATAATAC AGGAGTTACA 
GACCGAGTAG TTAACCAGTT GCTGACTCAG TTGGATGGAG TAGAAGGCTT ACAGGGTGTT 
TATGTATTGG CTGCTACTAG TCGCCCTGAC TTGATTGACC CTGCCCTGCT TAGGCCTGGT 
CGACTAGATA AATGTGTATA CTGTCCTCCT CCTGATCAGG TGTCACGTCT TGAAATTTTA 
AATGTCCTCA GTGACTCTCT ACCTCTGGCA GATGATGTTG ACCTTCAGCA TGTAGCATCA 
GTAACTGACT CCTTTACTGG AGCTGATCTG AAAGCTTTAC TTTACAATGC CCAATTGGAG 
GCCTTACATG GAATGCTGCT CTCGAGTGGA CTCCAGGATG GAAGTTCCAG CTCTGATAGT 
GACCTAAGTC TGTCTTCAAT GGTCTTTCTT AACCATAGCA GTGGCTCTGA CGATTCAGCT 
GGAGATGGAG AATGTGGCTT AGATCAGTCC CTTGTTTCTT TAGAGATGTC CGAGATCCTT 
CCAGATGAAT CAAAATTCAA TATGTACCGG CTCTACTTTG GAAGCTCTTA TGAATCAGAA 
CTTGGAAATG GAACCTCTTC TGATTTGAGC TCACAATGTC TCTCTGCACC AAGCTCCATG 
ACTCAGGATT TGCCTGGAGT TCCTGGGAAA GACCAGTTGT TTTCACAGCC TCCAGTGTTA 
AGGACAGCTT CACAAGAGGG TTGCCAAGAA CTTACACAAG AACAAAGAGA TCAACTGAGG 
GCAGATATCA GTATTATCAA AGGCAGATAC CGGAGCCAAA GTGGAGAGGA CGAATCCATG 
AACCAACCAG GACCAATCAA AACCAGACTG GCTATTAGTC AGTCACATTT AATGACTGCA 
CTTGGTCACA CAAGACCATC CATTAGTGAA GATGACTGGA AGAATTTTGC TGAGCTATAT 
GAAAGCTTTC AAAATCCAAA GAGGAGAAAA AATCAAAGTG GAACAATGTT TCGACCTGGA 
CAGAAAGTAA CTTTAGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Exome sequencing reveals novel genetic loci influencing obesity-related traits in Hispanic children.
Obesity (Silver Spring, Md.)25(7)1270-1276(2017 Jul)
Sabo A,Mishra P,Dugan-Perez S,Voruganti VS,Kent JW Jr,Kalra D,Cole SA,Comuzzie AG,Muzny DM,Gibbs RA,Butte NF

Novel compound heterozygous mutations in the PEX1 gene in two Chinese newborns with Zellweger syndrome based on whole exome sequencing.
Clinica chimica acta; international journal of clinical chemistry47024-28(2017 Jul)
Ge MM,Hu L,Li Z,Cheng G,Yan K,Kong Y,Wang H,Yang L,Zhou W

Spectrum of PEX1 and PEX6 variants in Heimler syndrome.
European journal of human genetics : EJHG24(11)1565-1571(2016 11)
Smith CE,Poulter JA,Levin AV,Capasso JE,Price S,Ben-Yosef T,Sharony R,Newman WG,Shore RC,Brookes SJ,Mighell AJ,Inglehearn CF

Severe early onset retinitis pigmentosa in a Moroccan patient with Heimler syndrome due to novel homozygous mutation of PEX1 gene.
European journal of medical genetics59(10)507-11(2016 Oct)
Ratbi I,Jaouad IC,Elorch H,Al-Sheqaih N,Elalloussi M,Lyahyai J,Berraho A,Newman WG,Sefiani A

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PEX1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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