IL20 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	IL20
Entrez Gene ID	50604
Full Name	interleukin 20
Synonyms	IL-20,IL10D,ZCYTO10
General protein information	Preferred Names interleukin 20 Names interleukin-20 cytokine Zcyto10 four alpha helix cytokine
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.1
Summary	The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. [provided by RefSeq, Jul 2008].
Disorder MIM:	605619

mRNA and Protein(s)

mRNA	Protein	Name
XM_005273152.3	XP_005273209.1	interleukin-20 isoform X1
NM_018724.3	NP_061194.2	interleukin-20 precursor

Pathways from BioSystems

Homologies

	IL20	XP_001084726.2
Rattus norvegicus (Norway rat)	Il20	NP_001137353.1
Gallus gallus (chicken)	IL19	XP_425824.2
Canis lupus familiaris (dog)	IL20	XP_851510.1
Pan troglodytes (chimpanzee)	IL20	XP_001165196.2
Bos taurus (cattle)	IL20	XP_002693938.2
Homo sapiens (human)	IL20	NP_061194.2
Mus musculus (house mouse)	Il20	NP_067355.1
Danio rerio (zebrafish)	il34	NP_001076424.1

Gene Ontology
Bibliography

Related articles in PubMed

The IL20 Genetic Polymorphism Is Associated with Altered Clinical Outcome in Septic Shock.
Nakada TA, Wacharasint P, Russell JA, Boyd JH, Nakada E, Thair SA, Shimada T, Walley KR
Journal of innate immunity(2018 Jan)

Interleukin-20 is triggered by TLR ligands and associates with disease activity in patients with rheumatoid arthritis.
?enolt L, Prajzlerov? K, Hulejov? H, ?umov? B, Filkov? M, Veigl D, Pavelka K, Vencovsk? J
Cytokine97187-192(2017 Sep)

A transcriptional complex composed of ER(?), GATA3, FOXA1 and ELL3 regulates IL-20 expression in breast cancer cells.
Lee JY, Park YJ, Oh N, Kwack KB, Park KS
Oncotarget8(26)42752-42760(2017 Jun)

IL-20 Signaling in Activated Human Neutrophils Inhibits Neutrophil Migration and Function.
Gough P, Ganesan S, Datta SK
Journal of immunology (Baltimore, Md. : 1950)198(11)4373-4382(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IL-20 and IL-24 increased the production of monocyte chemoattractant protein-1 by activated spondyloarthritis synovial fluid monocytes, decreased the production of Dickkopf-1 by SpA fibroblast-like synovial cells and induced mineralization in human osteoblasts; taken together, findings indicate disease-aggravating functions of IL-20 and IL-24 in spondyloarthritis
Title: Increased interleukin (IL)-20 and IL-24 target osteoblasts and synovial monocytes in spondyloarthritis.

The current study provides evidence that IL-20 directly targets and inhibits key inflammatory functions of neutrophils during infection with S. aureus.
Title: IL-20 Signaling in Activated Human Neutrophils Inhibits Neutrophil Migration and Function.

Results found that IL-20 is more specifically associated with ER-positive breast cancer and is epigenetically regulated by estrogen via the specific recruitment of KMT2B by ERalpha.
Title: Regulation of IL-20 Expression by Estradiol through KMT2B-Mediated Epigenetic Modification.

Data suggest that HGF, IL-20, and IL-22 in the serum and bronchoalveolar lavage fluid (BALF) of non-small cell lung cancer (NSCLC) patients before chemotherapy may be a prognostic of cancer progression.
Title: Clinical Implications of Hepatocyte Growth Factor, Interleukin-20, and Interleukin-22 in Serum and Bronchoalveolar Fluid of Patients with Non-Small Cell Lung Cancer.

Data show reduced cytokines IL-10 and IL-19 expression, and enhanced IL-20 and il-1 beta expression in chronic recurrent multifocal osteomyelitis (CRMO) monocytes.
Title: Altered expression of IL-10 family cytokines in monocytes from CRMO patients result in enhanced IL-1? expression and release.

The following IL20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu14079	NM_018724.3 Latest version!	Homo sapiens interleukin 20 (IL20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu34421	XM_005273152.3 Latest version!	Homo sapiens interleukin 20 (IL20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL20 ( NM_018724.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_061194.2 Homo sapiens interleukin 20 (IL20), mRNA.

Price & Availability↑

CloneID	OHu14079
Clone ID Related Accession (Same CDS sequence)	NM_018724.3
Accession Version	NM_018724.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	interleukin-20 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF224266.1, BC069311.1 and AY358320.1. On Jul 30, 2004 this sequence version replaced NM_018724.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF224266.1, BC117386.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMN02400288 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAAGCCT CTAGTCTTGC CTTCAGCCTT CTCTCTGCTG CGTTTTATCT CCTATGGACT 
CCTTCCACTG GACTGAAGAC ACTCAATTTG GGAAGCTGTG TGATCGCCAC AAACCTTCAG 
GAAATACGAA ATGGATTTTC TGAGATACGG GGCAGTGTGC AAGCCAAAGA TGGAAACATT 
GACATCAGAA TCTTAAGGAG GACTGAGTCT TTGCAAGACA CAAAGCCTGC GAATCGATGC 
TGCCTCCTGC GCCATTTGCT AAGACTCTAT CTGGACAGGG TATTTAAAAA CTACCAGACC 
CCTGACCATT ATACTCTCCG GAAGATCAGC AGCCTCGCCA ATTCCTTTCT TACCATCAAG 
AAGGACCTCC GGCTCTGTCA TGCCCACATG ACATGCCATT GTGGGGAGGA AGCAATGAAG 
AAATACAGCC AGATTCTGAG TCACTTTGAA AAGCTGGAAC CTCAGGCAGC AGTTGTGAAG 
GCTTTGGGGG AACTAGACAT TCTTCTGCAA TGGATGGAGG AGACAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061194.2
CDS	45..575
Translation	MKASSLAFSLLSAAFYLLWTPSTGLKTLNLGSCVIATNLQEIRNGFSEIRGSVQAKDGNIDIRILRRTESLQDTKPANRCCLLRHLLRLYLDRVFKNYQTPDHYTLRKISSLANSFLTIKKDLRLCHAHMTCHCGEEAMKKYSQILSHFEKLEPQAAVVKALGELDILLQWMEETE

Target ORF information:

RefSeq Version	NM_018724.3
Organism	Homo sapiens(human)
Definition	Homo sapiens interleukin 20 (IL20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018724.3

ORF Insert Sequence:

ATGAAAGCCT CTAGTCTTGC CTTCAGCCTT CTCTCTGCTG CGTTTTATCT CCTATGGACT 
CCTTCCACTG GACTGAAGAC ACTCAATTTG GGAAGCTGTG TGATCGCCAC AAACCTTCAG 
GAAATACGAA ATGGATTTTC TGAGATACGG GGCAGTGTGC AAGCCAAAGA TGGAAACATT 
GACATCAGAA TCTTAAGGAG GACTGAGTCT TTGCAAGACA CAAAGCCTGC GAATCGATGC 
TGCCTCCTGC GCCATTTGCT AAGACTCTAT CTGGACAGGG TATTTAAAAA CTACCAGACC 
CCTGACCATT ATACTCTCCG GAAGATCAGC AGCCTCGCCA ATTCCTTTCT TACCATCAAG 
AAGGACCTCC GGCTCTGTCA TGCCCACATG ACATGCCATT GTGGGGAGGA AGCAATGAAG 
AAATACAGCC AGATTCTGAG TCACTTTGAA AAGCTGGAAC CTCAGGCAGC AGTTGTGAAG 
GCTTTGGGGG AACTAGACAT TCTTCTGCAA TGGATGGAGG AGACAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IL20 ( XM_005273152.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273209.1 Homo sapiens interleukin 20 (IL20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu34421
Clone ID Related Accession (Same CDS sequence)	XM_005273152.3
Accession Version	XM_005273152.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	interleukin-20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005273152.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTCAAT TCCCCAAAAC AAGTTTTGAC ATTTCCCCTG AAATGTCATT CTCTATCTAT 
TCACTGCAAG TGCCTGCTGT TCCAGGCCTT ACCTGCTGGG CACTAACGGC GGAGCCAGGA 
TGGGGACAGA ATAAAGGAGC CACGACCTGT GCCACCAACT CGCACTCAGA CTCTGAACTC 
AGACCTGAAA TCTTCTCTTC ACGGGAGGCT TGGCAGTTTT TCTTACTCCT GTGGTCTCCA 
GATTTCAGGC CTAAGATGAA AGCCTCTAGT CTTGCCTTCA GCCTTCTCTC TGCTGCGTTT 
TATCTCCTAT GGACTCCTTC CACTGGACTG AAGACACTCA ATTTGGGAAG CTGTGTGATC 
GCCACAAACC TTCAGGAAAT ACGAAATGGA TTTTCTGAGA TACGGGGCAG TGTGCAAGCC 
AAAGATGGAA ACATTGACAT CAGAATCTTA AGGAGGACTG AGTCTTTGCA AGACACAAAG 
CCTGCGAATC GATGCTGCCT CCTGCGCCAT TTGCTAAGAC TCTATCTGGA CAGGGTATTT 
AAAAACTACC AGACCCCTGA CCATTATACT CTCCGGAAGA TCAGCAGCCT CGCCAATTCC 
TTTCTTACCA TCAAGAAGGA CCTCCGGCTC TGTCATGCCC ACATGACATG CCATTGTGGG 
GAGGAAGCAA TGAAGAAATA CAGCCAGATT CTGAGTCACT TTGAAAAGCT GGAACCTCAG 
GCAGCAGTTG TGAAGGCTTT GGGGGAACTA GACATTCTTC TGCAATGGAT GGAGGAGACA 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273209.1
CDS	216..1001
Translation	MRQFPKTSFDISPEMSFSIYSLQVPAVPGLTCWALTAEPGWGQNKGATTCATNSHSDSELRPEIFSSREAWQFFLLLWSPDFRPKMKASSLAFSLLSAAFYLLWTPSTGLKTLNLGSCVIATNLQEIRNGFSEIRGSVQAKDGNIDIRILRRTESLQDTKPANRCCLLRHLLRLYLDRVFKNYQTPDHYTLRKISSLANSFLTIKKDLRLCHAHMTCHCGEEAMKKYSQILSHFEKLEPQAAVVKALGELDILLQWMEETE

Target ORF information:

RefSeq Version	XM_005273152.3
Organism	Homo sapiens(human)
Definition	Homo sapiens interleukin 20 (IL20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273152.3

ORF Insert Sequence:

ATGCGTCAAT TCCCCAAAAC AAGTTTTGAC ATTTCCCCTG AAATGTCATT CTCTATCTAT 
TCACTGCAAG TGCCTGCTGT TCCAGGCCTT ACCTGCTGGG CACTAACGGC GGAGCCAGGA 
TGGGGACAGA ATAAAGGAGC CACGACCTGT GCCACCAACT CGCACTCAGA CTCTGAACTC 
AGACCTGAAA TCTTCTCTTC ACGGGAGGCT TGGCAGTTTT TCTTACTCCT GTGGTCTCCA 
GATTTCAGGC CTAAGATGAA AGCCTCTAGT CTTGCCTTCA GCCTTCTCTC TGCTGCGTTT 
TATCTCCTAT GGACTCCTTC CACTGGACTG AAGACACTCA ATTTGGGAAG CTGTGTGATC 
GCCACAAACC TTCAGGAAAT ACGAAATGGA TTTTCTGAGA TACGGGGCAG TGTGCAAGCC 
AAAGATGGAA ACATTGACAT CAGAATCTTA AGGAGGACTG AGTCTTTGCA AGACACAAAG 
CCTGCGAATC GATGCTGCCT CCTGCGCCAT TTGCTAAGAC TCTATCTGGA CAGGGTATTT 
AAAAACTACC AGACCCCTGA CCATTATACT CTCCGGAAGA TCAGCAGCCT CGCCAATTCC 
TTTCTTACCA TCAAGAAGGA CCTCCGGCTC TGTCATGCCC ACATGACATG CCATTGTGGG 
GAGGAAGCAA TGAAGAAATA CAGCCAGATT CTGAGTCACT TTGAAAAGCT GGAACCTCAG 
GCAGCAGTTG TGAAGGCTTT GGGGGAACTA GACATTCTTC TGCAATGGAT GGAGGAGACA 
GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The IL20 Genetic Polymorphism Is Associated with Altered Clinical Outcome in Septic Shock.
Journal of innate immunity(2018 Jan)
Nakada TA,Wacharasint P,Russell JA,Boyd JH,Nakada E,Thair SA,Shimada T,Walley KR

Interleukin-20 is triggered by TLR ligands and associates with disease activity in patients with rheumatoid arthritis.
Cytokine97187-192(2017 Sep)
?enolt L,Prajzlerov? K,Hulejov? H,?umov? B,Filkov? M,Veigl D,Pavelka K,Vencovsk? J

A transcriptional complex composed of ER(?), GATA3, FOXA1 and ELL3 regulates IL-20 expression in breast cancer cells.
Oncotarget8(26)42752-42760(2017 Jun)
Lee JY,Park YJ,Oh N,Kwack KB,Park KS

IL-20 Signaling in Activated Human Neutrophils Inhibits Neutrophil Migration and Function.
Journal of immunology (Baltimore, Md. : 1950)198(11)4373-4382(2017 Jun)
Gough P,Ganesan S,Datta SK