CISD2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CISD2
Entrez Gene ID	493856
Full Name	CDGSH iron sulfur domain 2
Synonyms	ERIS,Miner1,NAF-1,WFS2,ZCD2
General protein information	Preferred Names CDGSH iron sulfur domain 2 Names CDGSH iron-sulfur domain-containing protein 2 endoplasmic reticulum intermembrane small protein mitoNEET-related 1 protein nutrient-deprivation autophagy factor-1 zinc finger, CDGSH-type domain 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q24
Summary	The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2. [provided by RefSeq, Mar 2011].
Disorder MIM:	611507
Disorder Html:	Wolfram syndrome 2, 604928 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008388.5	NP_001008389.1	CDGSH iron-sulfur domain-containing protein 2
NM_001008388.4	NP_001008389.1	CDGSH iron-sulfur domain-containing protein 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	CISD2	NP_001008389.1
Rattus norvegicus (Norway rat)	Cisd2	NP_001178537.1
Danio rerio (zebrafish)	cisd2	NP_956677.1
Xenopus tropicalis (tropical clawed frog)	cisd2	NP_001011479.1
Pan troglodytes (chimpanzee)	CISD2	XP_003310469.1
Mus musculus (house mouse)	Cisd2	NP_080178.1
Gallus gallus (chicken)	CISD2	XP_420668.1
Drosophila melanogaster (fruit fly)	CG1458	NP_651684.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004484	XP_313782.3
	LOC100429989	XP_002804204.1
Bos taurus (cattle)	CISD2	NP_001073807.1

Gene Ontology
Bibliography

Related articles in PubMed

Generation of Human-Induced Pluripotent Stem Cells from Wolfram Syndrome Type 2 Patients Bearing the c.103?+?1G>A CISD2 Mutation for Disease Modeling.
La Spada A, Ntai A, Genovese S, Rondinelli M, De Blasio P, Biunno I
Stem cells and development(2018 Jan)

A donor splice site mutation in CISD2 generates multiple truncated, non-functional isoforms in Wolfram syndrome type 2 patients.
Cattaneo M, La Sala L, Rondinelli M, Errichiello E, Zuffardi O, Puca AA, Genovese S, Ceriello A
BMC medical genetics18(1)147(2017 12)

CISD2 promotes the proliferation of glioma cells via suppressing beclin?1?mediated autophagy and is targeted by microRNA?449a.
Sun AG, Meng FG, Wang MG
Molecular medicine reports16(6)7939-7948(2017 Dec)

CISD2 Haploinsufficiency Disrupts Calcium Homeostasis, Causes Nonalcoholic Fatty Liver Disease, and Promotes Hepatocellular Carcinoma.
Shen ZQ, Chen YF, Chen JR, Jou YS, Wu PC, Kao CH, Wang CH, Huang YL, Chen CF, Huang TS, Shyu YC, Tsai SF, Kao LS, Tsai TF
Cell reports21(8)2198-2211(2017 Nov)

Upregulation of CISD2 augments ROS homeostasis and contributes to tumorigenesis and poor prognosis of lung adenocarcinoma.
Li SM, Chen CH, Chen YW, Yen YC, Fang WT, Tsai FY, Chang JL, Shen YY, Huang SF, Chuu CP, Chang IS, Hsiung CA, Jiang SS
Scientific reports7(1)11893(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The c.103 + 1G > A mutation resulted in the loss of functional CISD2 protein in the two Italian Wolfram syndrome type 2 patients.
Title: A donor splice site mutation in CISD2 generates multiple truncated, non-functional isoforms in Wolfram syndrome type 2 patients.

the patient that we describe in this report with the c.215A > G missense CISD2 variant had the classical features of Wolfram syndrome type 1. The c.215A > G (p.Asn72Ser) variant does not induce CISD2 RNA mis-splicing or a reduction in CISD2 protein levels. Thus, we report a novel missense homozygous CISD2 mutation in a patient with clinical features that differ from previously reported Wolfram syndrome 2 case reports.
Title: A novel CISD2 mutation associated with a classical Wolfram syndrome phenotype alters Ca2+ homeostasis and ER-mitochondria interactions.

CISD2 could be an independent prognostic factor for PC and suggested that the CISD2/Wnt/beta-catenin pathway contributed to the proliferation of PC and EMT
Title: CDGSH Iron Sulfur Domain 2 Activates Proliferation and EMT of Pancreatic Cancer Cells via Wnt/?-Catenin Pathway and Has Prognostic Value in Human Pancreatic Cancer.

CISD2 was up-regulated in laryngeal squamous cell carcinoma
Title: A novel prognostic score model incorporating CDGSH iron sulfur domain2 (CISD2) predicts risk of disease progression in laryngeal squamous cell carcinoma.

CISD2 protein may serve as a candidate prognostic marker and a novel therapeutic target for hepatocellular carcinoma (HCC) and play an important role in promoting proliferation and enhanced progression of HCC.
Title: CISD2 associated with proliferation indicates negative prognosis in patients with hepatocellular carcinoma.

The following CISD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CISD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23185	NM_001008388.5 Latest version!	Homo sapiens CDGSH iron sulfur domain 2 (CISD2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu23185	NM_001008388.4	Homo sapiens CDGSH iron sulfur domain 2 (CISD2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CISD2 ( NM_001008388.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001008389.1 Homo sapiens CDGSH iron sulfur domain 2 (CISD2), mRNA.

Price & Availability↑

CloneID	OHu23185
Clone ID Related Accession (Same CDS sequence)	NM_001008388.5 , NM_001008388.4
Accession Version	NM_001008388.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	CDGSH iron-sulfur domain-containing protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018797.4, AK292134.1, BX537971.1 and AC083826.7. This sequence is a reference standard in the RefSeqGene project. On Jul 31, 2018 this sequence version replaced NM_001008388.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.39033.1, SRR1803616.114659.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000273986.10/ ENSP00000273986.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGCTGG AGAGCGTGGC CCGTATCGTG AAGGTGCAGC TCCCTGCATA TCTGAAGCGG 
CTCCCAGTCC CTGAAAGCAT TACCGGGTTC GCTAGGCTCA CAGTTTCAGA ATGGCTTCGG 
TTATTGCCTT TCCTTGGTGT ACTCGCACTT CTTGGCTACC TTGCAGTTCG TCCATTCCTC 
CCGAAGAAGA AACAACAGAA GGATAGCTTG ATTAATCTTA AAATACAAAA GGAAAATCCG 
AAAGTAGTGA ATGAAATAAA CATTGAAGAT TTGTGTCTTA CTAAAGCAGC TTATTGTAGG 
TGTTGGCGTT CTAAAACGTT TCCTGCCTGC GATGGTTCAC ATAATAAACA CAATGAATTG 
ACAGGAGATA ATGTGGGTCC ACTAATACTG AAGAAGAAAG AAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008389.1
CDS	94..501
Translation	MVLESVARIVKVQLPAYLKRLPVPESITGFARLTVSEWLRLLPFLGVLALLGYLAVRPFLPKKKQQKDSLINLKIQKENPKVVNEINIEDLCLTKAAYCRCWRSKTFPACDGSHNKHNELTGDNVGPLILKKKEV

Target ORF information:

RefSeq Version	NM_001008388.5
Organism	Homo sapiens(human)
Definition	Homo sapiens CDGSH iron sulfur domain 2 (CISD2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008388.5

ORF Insert Sequence:

ATGGTGCTGG AGAGCGTGGC CCGTATCGTG AAGGTGCAGC TCCCTGCATA TCTGAAGCGG 
CTCCCAGTCC CTGAAAGCAT TACCGGGTTC GCTAGGCTCA CAGTTTCAGA ATGGCTTCGG 
TTATTGCCTT TCCTTGGTGT ACTCGCACTT CTTGGCTACC TTGCAGTTCG TCCATTCCTC 
CCGAAGAAGA AACAACAGAA GGATAGCTTG ATTAATCTTA AAATACAAAA GGAAAATCCG 
AAAGTAGTGA ATGAAATAAA CATTGAAGAT TTGTGTCTTA CTAAAGCAGC TTATTGTAGG 
TGTTGGCGTT CTAAAACGTT TCCTGCCTGC GATGGTTCAC ATAATAAACA CAATGAATTG 
ACAGGAGATA ATGTGGGTCC ACTAATACTG AAGAAGAAAG AAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CISD2 ( NM_001008388.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001008389.1 Homo sapiens CDGSH iron sulfur domain 2 (CISD2), mRNA.

Price & Availability↑

CloneID	OHu23185
Clone ID Related Accession (Same CDS sequence)	NM_001008388.5 , NM_001008388.4
Accession Version	NM_001008388.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	CDGSH iron-sulfur domain-containing protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018797.4, AK292134.1, BX537971.1 and AC083826.7. This sequence is a reference standard in the RefSeqGene project. On Jul 3, 2010 this sequence version replaced NM_001008388.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.444246.1, SRR1660809.39033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCTGG AGAGCGTGGC CCGTATCGTG AAGGTGCAGC TCCCTGCATA TCTGAAGCGG 
CTCCCAGTCC CTGAAAGCAT TACCGGGTTC GCTAGGCTCA CAGTTTCAGA ATGGCTTCGG 
TTATTGCCTT TCCTTGGTGT ACTCGCACTT CTTGGCTACC TTGCAGTTCG TCCATTCCTC 
CCGAAGAAGA AACAACAGAA GGATAGCTTG ATTAATCTTA AAATACAAAA GGAAAATCCG 
AAAGTAGTGA ATGAAATAAA CATTGAAGAT TTGTGTCTTA CTAAAGCAGC TTATTGTAGG 
TGTTGGCGTT CTAAAACGTT TCCTGCCTGC GATGGTTCAC ATAATAAACA CAATGAATTG 
ACAGGAGATA ATGTGGGTCC ACTAATACTG AAGAAGAAAG AAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008389.1
CDS	108..515
Translation	MVLESVARIVKVQLPAYLKRLPVPESITGFARLTVSEWLRLLPFLGVLALLGYLAVRPFLPKKKQQKDSLINLKIQKENPKVVNEINIEDLCLTKAAYCRCWRSKTFPACDGSHNKHNELTGDNVGPLILKKKEV

Target ORF information:

RefSeq Version	NM_001008388.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CDGSH iron sulfur domain 2 (CISD2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008388.4

ORF Insert Sequence:

ATGGTGCTGG AGAGCGTGGC CCGTATCGTG AAGGTGCAGC TCCCTGCATA TCTGAAGCGG 
CTCCCAGTCC CTGAAAGCAT TACCGGGTTC GCTAGGCTCA CAGTTTCAGA ATGGCTTCGG 
TTATTGCCTT TCCTTGGTGT ACTCGCACTT CTTGGCTACC TTGCAGTTCG TCCATTCCTC 
CCGAAGAAGA AACAACAGAA GGATAGCTTG ATTAATCTTA AAATACAAAA GGAAAATCCG 
AAAGTAGTGA ATGAAATAAA CATTGAAGAT TTGTGTCTTA CTAAAGCAGC TTATTGTAGG 
TGTTGGCGTT CTAAAACGTT TCCTGCCTGC GATGGTTCAC ATAATAAACA CAATGAATTG 
ACAGGAGATA ATGTGGGTCC ACTAATACTG AAGAAGAAAG AAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation of Human-Induced Pluripotent Stem Cells from Wolfram Syndrome Type 2 Patients Bearing the c.103?+?1G>A CISD2 Mutation for Disease Modeling.
Stem cells and development(2018 Jan)
La Spada A,Ntai A,Genovese S,Rondinelli M,De Blasio P,Biunno I

A donor splice site mutation in CISD2 generates multiple truncated, non-functional isoforms in Wolfram syndrome type 2 patients.
BMC medical genetics18(1)147(2017 12)
Cattaneo M,La Sala L,Rondinelli M,Errichiello E,Zuffardi O,Puca AA,Genovese S,Ceriello A

CISD2 promotes the proliferation of glioma cells via suppressing beclin?1?mediated autophagy and is targeted by microRNA?449a.
Molecular medicine reports16(6)7939-7948(2017 Dec)
Sun AG,Meng FG,Wang MG

CISD2 Haploinsufficiency Disrupts Calcium Homeostasis, Causes Nonalcoholic Fatty Liver Disease, and Promotes Hepatocellular Carcinoma.
Cell reports21(8)2198-2211(2017 Nov)
Shen ZQ,Chen YF,Chen JR,Jou YS,Wu PC,Kao CH,Wang CH,Huang YL,Chen CF,Huang TS,Shyu YC,Tsai SF,Kao LS,Tsai TF

Upregulation of CISD2 augments ROS homeostasis and contributes to tumorigenesis and poor prognosis of lung adenocarcinoma.
Scientific reports7(1)11893(2017 Sep)
Li SM,Chen CH,Chen YW,Yen YC,Fang WT,Tsai FY,Chang JL,Shen YY,Huang SF,Chuu CP,Chang IS,Hsiung CA,Jiang SS