MYH8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH8
Entrez Gene ID	4626
Full Name	myosin heavy chain 8
Synonyms	DA7,MyHC-peri,MyHC-pn,gtMHC-F
General protein information	Preferred Names myosin heavy chain 8 Names myosin-8 fetal-myosin heavy chain myHC-perinatal myosin heavy chain, skeletal muscle, perinatal myosin, heavy chain 8, skeletal muscle, perinatal myosin, heavy polypeptide 8, skeletal muscle, perinatal
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.1
Summary	Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is predominantly expressed in fetal skeletal muscle. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in trismus-pseudocamptodactyly syndrome. [provided by RefSeq, Sep 2009].
Disorder MIM:	160741
Disorder Html:	Carney complex variant, 608837 (3); Trismus-pseudocamptodactyly syndrome, 158300 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002472.3	NP_002463.2	myosin-8
NM_002472.2	NP_002463.2	myosin-8

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Myh8	NP_001093955.1
Pan troglodytes (chimpanzee)	MYH8	XP_511839.2
Homo sapiens (human)	MYH8	NP_002463.2
Bos taurus (cattle)	MYH8	NP_001193103.1
Mus musculus (house mouse)	Myh8	NP_796343.2
	MYH8	XP_001113700.1
Canis lupus familiaris (dog)	MYH8	NP_001070703.1

Gene Ontology
Bibliography

Related articles in PubMed

Caution in interpretation of disease causality for heterozygous loss-of-function variants in the MYH8 gene associated with autosomal dominant disorder.
Dai Z, Whitt Z, Mighion LC, Pontoglio A, Bean LJH, Colombo R, Hegde M
European journal of medical genetics60(6)312-316(2017 Jun)

Rapid switch-off of the human myosin heavy chain IIX gene after heavy load muscle contractions is sustained for at least four days.
Andersen JL, Gruschy-Knudsen T
Scandinavian journal of medicine & science in sports(2017 May)

Characterization of human myoblast differentiation for tissue-engineering purposes by quantitative gene expression analysis.
Stern-Straeter J, Bonaterra GA, Kassner SS, Z?gel S, H?rmann K, Kinscherf R, Goessler UR
Journal of tissue engineering and regenerative medicine5(8)e197-206(2011 Aug)

Germline mosaicism for the c.2021G?>?A (p.Arg674Gln) mutation in siblings with trismus pseudocamptodactyly.
Bonapace G, Ceravolo F, Piccirillo A, Duro G, Strisciuglio P, Concolino D
American journal of medical genetics. Part A152A(11)2898-900(2010 Nov)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss-of-function variants in the MYH8 gene do not cause autosomal dominant trismus-pseudocamptodactyly syndrome.
Title: Caution in interpretation of disease causality for heterozygous loss-of-function variants in the MYH8 gene associated with autosomal dominant disorder.

a family in which two out three sibs affected with trismus pseudocamptodactyly, born from healthy nonconsanguineous parents, were heterozygous for the c.2021G > A mutation due to a possible germline mosaicism in MYH8
Title: Germline mosaicism for the c.2021G?>?A (p.Arg674Gln) mutation in siblings with trismus pseudocamptodactyly.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Evaluation of embryonic and perinatal myosin gene mutations and the etiology of congenital idiopathic clubfoot.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following MYH8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18881	NM_002472.3 Latest version!	Homo sapiens myosin heavy chain 8 (MYH8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
View Old Accession Versions >>
OHu18881	NM_002472.2	Homo sapiens myosin heavy chain 8 (MYH8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH8 ( NM_002472.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002463.2 Homo sapiens myosin heavy chain 8 (MYH8), mRNA.

Price & Availability↑

CloneID	OHu18881
Clone ID Related Accession (Same CDS sequence)	NM_002472.3 , NM_002472.2
Accession Version	NM_002472.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	myosin-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB142109.1, M36769.1, AC005323.1, Z38133.1 and AC005291.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002472.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: M36769.1, Z38133.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA1970526 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000403437.2/ ENSP00000384330.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTGCGA GCTCAGACGC TGAGATGGCT GTTTTTGGCG AAGCTGCTCC CTACCTTCGA 
AAATCAGAAA AGGAGCGGAT TGAGGCCCAA AACAAGCCGT TTGATGCTAA AACATCTGTC 
TTTGTGGCGG AGCCCAAGGA ATCCTATGTG AAGAGCACTA TACAAAGCAA AGAAGGAGGG 
AAAGTAACCG TAAAGACTGA AGGTGGAGCA ACTCTAACTG TCAGGGAAGA CCAAGTCTTC 
CCTATGAACC CTCCGAAATA TGACAAAATT GAGGACATGG CCATGATGAC TCATCTACAC 
GAGCCTGGAG TGCTGTACAA CCTCAAAGAG CGCTATGCAG CCTGGATGAT CTACACCTAC 
TCAGGCCTCT TCTGTGTCAC CGTCAACCCC TACAAGTGGC TGCCGGTGTA CAAGCCCGAG 
GTGGTGGCTG CCTACAGAGG CAAAAAGCGC CAGGAGGCCC CGCCCCACAT CTTCTCCATC 
TCTGACAATG CCTATCAGTT CATGTTGACT GATCGAGAGA ATCAGTCCAT CCTGATCACC 
GGAGAATCTG GTGCCGGAAA GACTGTGAAC ACCAAGCGTG TCATCCAATA CTTTGCAACA 
ATTGCAGTTA CTGGAGAGAA GAAGAAGGAT GAATCTGGCA AAATGCAGGG GACTCTGGAA 
GATCAAATCA TCAGCGCCAA TCCCCTACTG GAGGCCTTTG GCAATGCCAA AACTGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATTAGAATCC ACTTTGGTAC TACAGGGAAG 
CTGGCATCTG CTGATATAGA AACATATCTT TTAGAAAAGT CCAGAGTTAC TTTCCAGCTA 
AAGGCGGAAA GAAGCTACCA TATTTTTTAT CAGATCACTT CCAATAAGAA GCCAGATCTA 
ATTGAAATGC TCCTGATCAC CACCAACCCA TATGACTATG CCTTCGTCAG TCAGGGGGAG 
ATCACAGTTC CCAGTATTGA TGACCAAGAA GAGTTGATGG CCACTGATAG TGCCATTGAC 
ATCCTGGGCT TCACTCCTGA AGAGAAAGTG TCCATCTATA AACTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCTGA GCCAGATGGC 
ACAGAAGTCG CTGACAAGGC AGCCTATCTC CAGAGTCTGA ACTCTGCAGA CCTACTCAAA 
GCCCTCTGCT ACCCTAGGGT CAAGGTTGGC AATGAGTATG TCACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAATGCGGT GGGTGCTCTG GCCAAAGCCG TCTACGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAAATC TTTGATTTTA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTAGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGACGTTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCA CTGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCAAC GGACACCTCC TTCAAGAACA AGCTGTATGA CCAGCACCTG 
GGCAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAAG GCAAGGCTGA GGCCCACTTC 
TCTCTGATTC ACTATGCTGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAAAAT 
AAGGACCCCC TGAATGATAC TGTGGTTGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTA 
GCCAGTCTCT TTTCCACGTA TGCTAGTGCT GAAGCAGATA GCAGCGCGAA GAAAGGTGCT 
AAGAAAAAGG GCTCTTCTTT CCAGACTGTG TCTGCCCTTT TCAGGGAAAA TTTAAATAAA 
TTGATGACGA ATCTGAGGAG CACACACCCT CACTTCGTAC GGTGTATCAT TCCCAATGAA 
ACCAAAACTC CTGGGGCAAT GGAACATGAA CTTGTGTTGC ACCAGCTGAG GTGTAATGGT 
GTGCTGGAAG GCATCCGCAT CTGTAGGAAA GGATTCCCAA GCAGAATCTT ATATGGTGAT 
TTCAAACAAA GATACAAGGT TTTAAATGCA AGTGCTATTC CAGAGGGACA GTTCATTGAC 
AGCAAGAAGG CTTCTGAGAA ACTTCTTGCA TCTATTGATA TTGATCATAC TCAATATAAA 
TTTGGACATA CCAAGGTTTT CTTCAAAGCT GGACTTCTGG GTCTTCTGGA AGAAATGAGA 
GATGAAAAAT TAGCCCAAAT TATAACAAGA ACACAAGCTG TCTGTAGGGG ATTCCTAATG 
AGGGTAGAAT ATCAGAAGAT GTTGCAAAGG AGAGAAGCAC TTTTCTGCAT CCAGTATAAT 
GTCCGTGCCT TCATGAACGT CAAGCACTGG CCCTGGATGA AACTCTTTTT CAAGATTAAG 
CCCCTCCTCA AGAGTGCAGA GACCGAGAAA GAGATGGCCA CCATGAAGGA AGAATTCCAG 
AAAACCAAAG ATGAACTCGC CAAGTCAGAG GCAAAACGGA AGGAGCTAGA GGAAAAAATG 
GTCACTCTCT TAAAAGAGAA AAATGACCTG CAACTCCAGG TTCAATCTGA AGCAGATAGC 
TTGGCTGATG CAGAGGAAAG GTGTGAGCAA CTGATTAAAA ACAAAATCCA ACTTGAGGCC 
AAAATCAAAG AGGTGACTGA AAGAGCTGAG GAGGAGGAAG AGATCAATGC TGAGCTGACA 
GCCAAGAAGA GAAAACTGGA GGATGAATGT TCAGAACTCA AGAAAGACAT TGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACATGCCA CGGAGAACAA GGTGAAAAAT 
CTTACAGAAG AGATGGCAGG CCTGGATGAA ACCATTGCAA AACTGTCCAA GGAGAAGAAG 
GCTCTCCAAG AGACCCACCA GCAGACCCTG GATGACCTGC AGGCAGAGGA GGACAAAGTC 
AACATCCTGA CCAAAGCTAA AACCAAGCTA GAACAGCAAG TGGATGATCT TGAAGGGTCT 
CTGGAACAAG AAAAGAAGCT TCGAATGGAT CTAGAAAGAG CAAAGCGGAA ACTGGAGGGT 
GACCTCAAAT TGGCCCAAGA ATCCACAATG GATATGGAAA ATGACAAACA GCAACTTGAT 
GAAAAGCTTG AAAAGAAAGA ATTTGAAATC AGCAATTTGA TAAGCAAAAT TGAAGATGAG 
CAAGCTGTAG AAATTCAACT ACAGAAGAAG ATCAAAGAGT TGCAGGCCCG CATTGAGGAG 
CTGGGGGAAG AAATCGAGGC AGAGAGGGCG TCCCGAGCCA AAGCGGAGAA GCAGCGCTCT 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGAGGCTGG AAGAAGCCGG TGGGGCAACT 
TCTGCTCAGG TGGAATTGAA CAAGAAGCGG GAGGCTGAGT TTCAGAAACT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCATGAAGCT ATGGTGGCTG CTCTTCGGAA GAAGCACGCA 
GACAGTATGG CTGAGCTTGG GGAGCAGATT GACAACTTGC AGCGGGTCAA ACAGAAGCTG 
GAGAAGGAGA AGAGTGAGCT GAAGATGGAG ACTGATGACC TCAGCAGTAA CGCAGAGGCC 
ATTTCCAAAG CCAAGGGAAA CCTTGAAAAG ATGTGCCGCT CTCTAGAAGA TCAAGTGAGT 
GAGCTTAAGA CCAAGGAAGA GGAGCAGCAG CGGCTGATCA ATGACCTCAC AGCACAGAGA 
GCGCGCCTGC AGACAGAAGC GGGTGAATAT TCTCGACAAT TAGATGAGAA AGATGCTTTA 
GTCTCTCAGC TTTCAAGGAG CAAGCAAGCA TCTACTCAGC AGATTGAAGA GCTGAAACAT 
CAACTAGAGG AAGAAACTAA AGCCAAGAAC GCCCTGGCAC ACGCCCTGCA GTCCTCCCGC 
CATGACTGCG ACCTGCTGCG GGAACAGTAT GAGGAAGAGC AGGAAGGCAA AGCTGAGCTG 
CAGAGGGCGC TGTCCAAGGC CAACAGTGAG GTTGCCCAGT GGAGAACCAA ATACGAGACG 
GATGCCATCC AGCGCACAGA GGAGCTGGAG GAGGCCAAGA AAAAGTTGGC CCAGCGCCTG 
CAAGAAGCTG AGGAACATGT AGAAGCTGTG AACGCCAAAT GTGCTTCCCT TGAGAAGACG 
AAGCAGCGGC TCCAGAATGA AGTTGAAGAC CTCATGCTTG ATGTGGAAAG GTCTAATGCA 
GCCTGTGCAG CCCTTGATAA GAAGCAAAGG AACTTTGACA AGGTCCTATC AGAATGGAAG 
CAGAAGTATG AGGAAACTCA GGCTGAACTT GAGGCCTCCC AGAAGGAGTC ACGTTCTCTT 
AGCACTGAGC TGTTCAAGGT GAAGAATGTC TATGAGGAAT CCCTGGATCA ACTCGAAACG 
CTAAGAAGAG AAAATAAGAA CTTGCAACAG GAGATTTCTG ACCTCACTGA GCAGATTGCA 
GAGGGAGGAA AGCAAATTCA TGAATTGGAG AAAATAAAGA AGCAAGTAGA ACAAGAGAAA 
TGTGAAATTC AGGCTGCTTT AGAGGAAGCA GAGGCATCTC TTGAACATGA AGAAGGAAAG 
ATTCTGCGTA TCCAGCTTGA GTTAAACCAA GTCAAGTCTG AAGTTGATAG AAAAATCGCA 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGAAACCACA CTAGAGTCGT GGAGACAATG 
CAGAGCACGC TGGATGCAGA GATTAGAAGC AGAAATGATG CTCTGAGAGT CAAGAAGAAA 
ATGGAAGGAG ATCTGAATGA AATGGAAATC CAGCTGAACC ATGCCAATCG CTTAGCTGCA 
GAGAGTTTAA GGAACTACAG GAACACCCAA GGAATCCTGA AGGAAACCCA GCTCCACCTG 
GATGATGCTC TCCGGGGCCA GGAGGACCTC AAGGAACAGC TGGCAATTGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATCGAGGAG CTGTGGGCCA CTCTGGAACA GACAGAGAGA 
AGCAGGAAAA TCGCCGAACA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTCCAC 
ACCCAGAATA CCAGTCTCAT TAACACCAAG AAGAAATTAG AAAATGACGT TTCCCAACTC 
CAAAGTGAAG TGGAAGAAGT AATCCAAGAA TCACGCAATG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCTGCCAT GATGGCTGAG GAGCTGAAGA AGGAACAGGA CACCAGCGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCATCGTCTA 
GATGAGGCCG AGCAGCTGGC GCTGAAGGGT GGGAAGAAGC AGATCCAGAA ACTGGAGGCC 
AGGGTACGTG AGCTTGAAGG AGAGGTTGAA AATGAACAGA AACGTAATGC AGAGGCTGTT 
AAAGGTTTAC GGAAACATGA GCGACGAGTA AAAGAACTCA CCTACCAGAC TGAAGAAGAT 
CGCAAGAATG TTCTCAGGCT GCAGGACTTG GTAGATAAAT TACAGGCGAA GGTGAAATCA 
TACAAGAGAC AAGCTGAGGA GGCTGAGGAA CAATCCAATG CTAATCTATC TAAATTCCGC 
AAACTCCAGC ATGAGCTGGA GGAGGCCGAG GAACGGGCTG ACATTGCTGA GTCCCAGGTC 
AACAAATTGC GAGTGAAGAG CCGAGAGGTT CACACAAAAA TCAGTGCAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002463.2
CDS	96..5909
Translation	MSASSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVAEPKESYVKSTIQSKEGGKVTVKTEGGATLTVREDQVFPMNPPKYDKIEDMAMMTHLHEPGVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYKPEVVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKDESGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQITSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIDILGFTPEEKVSIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNAVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKSANFQKPKVVKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNDTVVGLYQKSAMKTLASLFSTYASAEADSSAKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQIITRTQAVCRGFLMRVEYQKMLQRREALFCIQYNVRAFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQSEADSLADAEERCEQLIKNKIQLEAKIKEVTERAEEEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLSKEKKALQETHQQTLDDLQAEEDKVNILTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESTMDMENDKQQLDEKLEKKEFEISNLISKIEDEQAVEIQLQKKIKELQARIEELGEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQVELNKKREAEFQKLRRDLEEATLQHEAMVAALRKKHADSMAELGEQIDNLQRVKQKLEKEKSELKMETDDLSSNAEAISKAKGNLEKMCRSLEDQVSELKTKEEEQQRLINDLTAQRARLQTEAGEYSRQLDEKDALVSQLSRSKQASTQQIEELKHQLEEETKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERSNAACAALDKKQRNFDKVLSEWKQKYEETQAELEASQKESRSLSTELFKVKNVYEESLDQLETLRRENKNLQQEISDLTEQIAEGGKQIHELEKIKKQVEQEKCEIQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQLKRNHTRVVETMQSTLDAEIRSRNDALRVKKKMEGDLNEMEIQLNHANRLAAESLRNYRNTQGILKETQLHLDDALRGQEDLKEQLAIVERRANLLQAEIEELWATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLENDVSQLQSEVEEVIQESRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVENEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQAKVKSYKRQAEEAEEQSNANLSKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKISAE

Target ORF information:

RefSeq Version	NM_002472.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 8 (MYH8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002472.3

ORF Insert Sequence:

ATGAGTGCGA GCTCAGACGC TGAGATGGCT GTTTTTGGCG AAGCTGCTCC CTACCTTCGA 
AAATCAGAAA AGGAGCGGAT TGAGGCCCAA AACAAGCCGT TTGATGCTAA AACATCTGTC 
TTTGTGGCGG AGCCCAAGGA ATCCTATGTG AAGAGCACTA TACAAAGCAA AGAAGGAGGG 
AAAGTAACCG TAAAGACTGA AGGTGGAGCA ACTCTAACTG TCAGGGAAGA CCAAGTCTTC 
CCTATGAACC CTCCGAAATA TGACAAAATT GAGGACATGG CCATGATGAC TCATCTACAC 
GAGCCTGGAG TGCTGTACAA CCTCAAAGAG CGCTATGCAG CCTGGATGAT CTACACCTAC 
TCAGGCCTCT TCTGTGTCAC CGTCAACCCC TACAAGTGGC TGCCGGTGTA CAAGCCCGAG 
GTGGTGGCTG CCTACAGAGG CAAAAAGCGC CAGGAGGCCC CGCCCCACAT CTTCTCCATC 
TCTGACAATG CCTATCAGTT CATGTTGACT GATCGAGAGA ATCAGTCCAT CCTGATCACC 
GGAGAATCTG GTGCCGGAAA GACTGTGAAC ACCAAGCGTG TCATCCAATA CTTTGCAACA 
ATTGCAGTTA CTGGAGAGAA GAAGAAGGAT GAATCTGGCA AAATGCAGGG GACTCTGGAA 
GATCAAATCA TCAGCGCCAA TCCCCTACTG GAGGCCTTTG GCAATGCCAA AACTGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATTAGAATCC ACTTTGGTAC TACAGGGAAG 
CTGGCATCTG CTGATATAGA AACATATCTT TTAGAAAAGT CCAGAGTTAC TTTCCAGCTA 
AAGGCGGAAA GAAGCTACCA TATTTTTTAT CAGATCACTT CCAATAAGAA GCCAGATCTA 
ATTGAAATGC TCCTGATCAC CACCAACCCA TATGACTATG CCTTCGTCAG TCAGGGGGAG 
ATCACAGTTC CCAGTATTGA TGACCAAGAA GAGTTGATGG CCACTGATAG TGCCATTGAC 
ATCCTGGGCT TCACTCCTGA AGAGAAAGTG TCCATCTATA AACTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCTGA GCCAGATGGC 
ACAGAAGTCG CTGACAAGGC AGCCTATCTC CAGAGTCTGA ACTCTGCAGA CCTACTCAAA 
GCCCTCTGCT ACCCTAGGGT CAAGGTTGGC AATGAGTATG TCACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAATGCGGT GGGTGCTCTG GCCAAAGCCG TCTACGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAAATC TTTGATTTTA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTAGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGACGTTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCA CTGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCAAC GGACACCTCC TTCAAGAACA AGCTGTATGA CCAGCACCTG 
GGCAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAAG GCAAGGCTGA GGCCCACTTC 
TCTCTGATTC ACTATGCTGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAAAAT 
AAGGACCCCC TGAATGATAC TGTGGTTGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTA 
GCCAGTCTCT TTTCCACGTA TGCTAGTGCT GAAGCAGATA GCAGCGCGAA GAAAGGTGCT 
AAGAAAAAGG GCTCTTCTTT CCAGACTGTG TCTGCCCTTT TCAGGGAAAA TTTAAATAAA 
TTGATGACGA ATCTGAGGAG CACACACCCT CACTTCGTAC GGTGTATCAT TCCCAATGAA 
ACCAAAACTC CTGGGGCAAT GGAACATGAA CTTGTGTTGC ACCAGCTGAG GTGTAATGGT 
GTGCTGGAAG GCATCCGCAT CTGTAGGAAA GGATTCCCAA GCAGAATCTT ATATGGTGAT 
TTCAAACAAA GATACAAGGT TTTAAATGCA AGTGCTATTC CAGAGGGACA GTTCATTGAC 
AGCAAGAAGG CTTCTGAGAA ACTTCTTGCA TCTATTGATA TTGATCATAC TCAATATAAA 
TTTGGACATA CCAAGGTTTT CTTCAAAGCT GGACTTCTGG GTCTTCTGGA AGAAATGAGA 
GATGAAAAAT TAGCCCAAAT TATAACAAGA ACACAAGCTG TCTGTAGGGG ATTCCTAATG 
AGGGTAGAAT ATCAGAAGAT GTTGCAAAGG AGAGAAGCAC TTTTCTGCAT CCAGTATAAT 
GTCCGTGCCT TCATGAACGT CAAGCACTGG CCCTGGATGA AACTCTTTTT CAAGATTAAG 
CCCCTCCTCA AGAGTGCAGA GACCGAGAAA GAGATGGCCA CCATGAAGGA AGAATTCCAG 
AAAACCAAAG ATGAACTCGC CAAGTCAGAG GCAAAACGGA AGGAGCTAGA GGAAAAAATG 
GTCACTCTCT TAAAAGAGAA AAATGACCTG CAACTCCAGG TTCAATCTGA AGCAGATAGC 
TTGGCTGATG CAGAGGAAAG GTGTGAGCAA CTGATTAAAA ACAAAATCCA ACTTGAGGCC 
AAAATCAAAG AGGTGACTGA AAGAGCTGAG GAGGAGGAAG AGATCAATGC TGAGCTGACA 
GCCAAGAAGA GAAAACTGGA GGATGAATGT TCAGAACTCA AGAAAGACAT TGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACATGCCA CGGAGAACAA GGTGAAAAAT 
CTTACAGAAG AGATGGCAGG CCTGGATGAA ACCATTGCAA AACTGTCCAA GGAGAAGAAG 
GCTCTCCAAG AGACCCACCA GCAGACCCTG GATGACCTGC AGGCAGAGGA GGACAAAGTC 
AACATCCTGA CCAAAGCTAA AACCAAGCTA GAACAGCAAG TGGATGATCT TGAAGGGTCT 
CTGGAACAAG AAAAGAAGCT TCGAATGGAT CTAGAAAGAG CAAAGCGGAA ACTGGAGGGT 
GACCTCAAAT TGGCCCAAGA ATCCACAATG GATATGGAAA ATGACAAACA GCAACTTGAT 
GAAAAGCTTG AAAAGAAAGA ATTTGAAATC AGCAATTTGA TAAGCAAAAT TGAAGATGAG 
CAAGCTGTAG AAATTCAACT ACAGAAGAAG ATCAAAGAGT TGCAGGCCCG CATTGAGGAG 
CTGGGGGAAG AAATCGAGGC AGAGAGGGCG TCCCGAGCCA AAGCGGAGAA GCAGCGCTCT 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGAGGCTGG AAGAAGCCGG TGGGGCAACT 
TCTGCTCAGG TGGAATTGAA CAAGAAGCGG GAGGCTGAGT TTCAGAAACT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCATGAAGCT ATGGTGGCTG CTCTTCGGAA GAAGCACGCA 
GACAGTATGG CTGAGCTTGG GGAGCAGATT GACAACTTGC AGCGGGTCAA ACAGAAGCTG 
GAGAAGGAGA AGAGTGAGCT GAAGATGGAG ACTGATGACC TCAGCAGTAA CGCAGAGGCC 
ATTTCCAAAG CCAAGGGAAA CCTTGAAAAG ATGTGCCGCT CTCTAGAAGA TCAAGTGAGT 
GAGCTTAAGA CCAAGGAAGA GGAGCAGCAG CGGCTGATCA ATGACCTCAC AGCACAGAGA 
GCGCGCCTGC AGACAGAAGC GGGTGAATAT TCTCGACAAT TAGATGAGAA AGATGCTTTA 
GTCTCTCAGC TTTCAAGGAG CAAGCAAGCA TCTACTCAGC AGATTGAAGA GCTGAAACAT 
CAACTAGAGG AAGAAACTAA AGCCAAGAAC GCCCTGGCAC ACGCCCTGCA GTCCTCCCGC 
CATGACTGCG ACCTGCTGCG GGAACAGTAT GAGGAAGAGC AGGAAGGCAA AGCTGAGCTG 
CAGAGGGCGC TGTCCAAGGC CAACAGTGAG GTTGCCCAGT GGAGAACCAA ATACGAGACG 
GATGCCATCC AGCGCACAGA GGAGCTGGAG GAGGCCAAGA AAAAGTTGGC CCAGCGCCTG 
CAAGAAGCTG AGGAACATGT AGAAGCTGTG AACGCCAAAT GTGCTTCCCT TGAGAAGACG 
AAGCAGCGGC TCCAGAATGA AGTTGAAGAC CTCATGCTTG ATGTGGAAAG GTCTAATGCA 
GCCTGTGCAG CCCTTGATAA GAAGCAAAGG AACTTTGACA AGGTCCTATC AGAATGGAAG 
CAGAAGTATG AGGAAACTCA GGCTGAACTT GAGGCCTCCC AGAAGGAGTC ACGTTCTCTT 
AGCACTGAGC TGTTCAAGGT GAAGAATGTC TATGAGGAAT CCCTGGATCA ACTCGAAACG 
CTAAGAAGAG AAAATAAGAA CTTGCAACAG GAGATTTCTG ACCTCACTGA GCAGATTGCA 
GAGGGAGGAA AGCAAATTCA TGAATTGGAG AAAATAAAGA AGCAAGTAGA ACAAGAGAAA 
TGTGAAATTC AGGCTGCTTT AGAGGAAGCA GAGGCATCTC TTGAACATGA AGAAGGAAAG 
ATTCTGCGTA TCCAGCTTGA GTTAAACCAA GTCAAGTCTG AAGTTGATAG AAAAATCGCA 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGAAACCACA CTAGAGTCGT GGAGACAATG 
CAGAGCACGC TGGATGCAGA GATTAGAAGC AGAAATGATG CTCTGAGAGT CAAGAAGAAA 
ATGGAAGGAG ATCTGAATGA AATGGAAATC CAGCTGAACC ATGCCAATCG CTTAGCTGCA 
GAGAGTTTAA GGAACTACAG GAACACCCAA GGAATCCTGA AGGAAACCCA GCTCCACCTG 
GATGATGCTC TCCGGGGCCA GGAGGACCTC AAGGAACAGC TGGCAATTGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATCGAGGAG CTGTGGGCCA CTCTGGAACA GACAGAGAGA 
AGCAGGAAAA TCGCCGAACA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTCCAC 
ACCCAGAATA CCAGTCTCAT TAACACCAAG AAGAAATTAG AAAATGACGT TTCCCAACTC 
CAAAGTGAAG TGGAAGAAGT AATCCAAGAA TCACGCAATG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCTGCCAT GATGGCTGAG GAGCTGAAGA AGGAACAGGA CACCAGCGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCATCGTCTA 
GATGAGGCCG AGCAGCTGGC GCTGAAGGGT GGGAAGAAGC AGATCCAGAA ACTGGAGGCC 
AGGGTACGTG AGCTTGAAGG AGAGGTTGAA AATGAACAGA AACGTAATGC AGAGGCTGTT 
AAAGGTTTAC GGAAACATGA GCGACGAGTA AAAGAACTCA CCTACCAGAC TGAAGAAGAT 
CGCAAGAATG TTCTCAGGCT GCAGGACTTG GTAGATAAAT TACAGGCGAA GGTGAAATCA 
TACAAGAGAC AAGCTGAGGA GGCTGAGGAA CAATCCAATG CTAATCTATC TAAATTCCGC 
AAACTCCAGC ATGAGCTGGA GGAGGCCGAG GAACGGGCTG ACATTGCTGA GTCCCAGGTC 
AACAAATTGC GAGTGAAGAG CCGAGAGGTT CACACAAAAA TCAGTGCAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH8 ( NM_002472.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002463.2 Homo sapiens myosin heavy chain 8 (MYH8), mRNA.

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CloneID	OHu18881
Clone ID Related Accession (Same CDS sequence)	NM_002472.3 , NM_002472.2
Accession Version	NM_002472.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	myosin-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB142109.1, M36769.1, AC005323.1 and Z38133.1. This sequence is a reference standard in the RefSeqGene project. On Jul 26, 2007 this sequence version replaced NM_002472.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: M36769.1, Z38133.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA1970526 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGCGA GCTCAGACGC TGAGATGGCT GTTTTTGGCG AAGCTGCTCC CTACCTTCGA 
AAATCAGAAA AGGAGCGGAT TGAGGCCCAA AACAAGCCGT TTGATGCTAA AACATCTGTC 
TTTGTGGCGG AGCCCAAGGA ATCCTATGTG AAGAGCACTA TACAAAGCAA AGAAGGAGGG 
AAAGTAACCG TAAAGACTGA AGGTGGAGCA ACTCTAACTG TCAGGGAAGA CCAAGTCTTC 
CCTATGAACC CTCCGAAATA TGACAAAATT GAGGACATGG CCATGATGAC TCATCTACAC 
GAGCCTGGAG TGCTGTACAA CCTCAAAGAG CGCTATGCAG CCTGGATGAT CTACACCTAC 
TCAGGCCTCT TCTGTGTCAC CGTCAACCCC TACAAGTGGC TGCCGGTGTA CAAGCCCGAG 
GTGGTGGCTG CCTACAGAGG CAAAAAGCGC CAGGAGGCCC CGCCCCACAT CTTCTCCATC 
TCTGACAATG CCTATCAGTT CATGTTGACT GATCGAGAGA ATCAGTCCAT CCTGATCACC 
GGAGAATCTG GTGCCGGAAA GACTGTGAAC ACCAAGCGTG TCATCCAATA CTTTGCAACA 
ATTGCAGTTA CTGGAGAGAA GAAGAAGGAT GAATCTGGCA AAATGCAGGG GACTCTGGAA 
GATCAAATCA TCAGCGCCAA TCCCCTACTG GAGGCCTTTG GCAATGCCAA AACTGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATTAGAATCC ACTTTGGTAC TACAGGGAAG 
CTGGCATCTG CTGATATAGA AACATATCTT TTAGAAAAGT CCAGAGTTAC TTTCCAGCTA 
AAGGCGGAAA GAAGCTACCA TATTTTTTAT CAGATCACTT CCAATAAGAA GCCAGATCTA 
ATTGAAATGC TCCTGATCAC CACCAACCCA TATGACTATG CCTTCGTCAG TCAGGGGGAG 
ATCACAGTTC CCAGTATTGA TGACCAAGAA GAGTTGATGG CCACTGATAG TGCCATTGAC 
ATCCTGGGCT TCACTCCTGA AGAGAAAGTG TCCATCTATA AACTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCTGA GCCAGATGGC 
ACAGAAGTCG CTGACAAGGC AGCCTATCTC CAGAGTCTGA ACTCTGCAGA CCTACTCAAA 
GCCCTCTGCT ACCCTAGGGT CAAGGTTGGC AATGAGTATG TCACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAATGCGGT GGGTGCTCTG GCCAAAGCCG TCTACGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAAATC TTTGATTTTA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTAGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGACGTTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCA CTGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCAAC GGACACCTCC TTCAAGAACA AGCTGTATGA CCAGCACCTG 
GGCAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAAG GCAAGGCTGA GGCCCACTTC 
TCTCTGATTC ACTATGCTGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAAAAT 
AAGGACCCCC TGAATGATAC TGTGGTTGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTA 
GCCAGTCTCT TTTCCACGTA TGCTAGTGCT GAAGCAGATA GCAGCGCGAA GAAAGGTGCT 
AAGAAAAAGG GCTCTTCTTT CCAGACTGTG TCTGCCCTTT TCAGGGAAAA TTTAAATAAA 
TTGATGACGA ATCTGAGGAG CACACACCCT CACTTCGTAC GGTGTATCAT TCCCAATGAA 
ACCAAAACTC CTGGGGCAAT GGAACATGAA CTTGTGTTGC ACCAGCTGAG GTGTAATGGT 
GTGCTGGAAG GCATCCGCAT CTGTAGGAAA GGATTCCCAA GCAGAATCTT ATATGGTGAT 
TTCAAACAAA GATACAAGGT TTTAAATGCA AGTGCTATTC CAGAGGGACA GTTCATTGAC 
AGCAAGAAGG CTTCTGAGAA ACTTCTTGCA TCTATTGATA TTGATCATAC TCAATATAAA 
TTTGGACATA CCAAGGTTTT CTTCAAAGCT GGACTTCTGG GTCTTCTGGA AGAAATGAGA 
GATGAAAAAT TAGCCCAAAT TATAACAAGA ACACAAGCTG TCTGTAGGGG ATTCCTAATG 
AGGGTAGAAT ATCAGAAGAT GTTGCAAAGG AGAGAAGCAC TTTTCTGCAT CCAGTATAAT 
GTCCGTGCCT TCATGAACGT CAAGCACTGG CCCTGGATGA AACTCTTTTT CAAGATTAAG 
CCCCTCCTCA AGAGTGCAGA GACCGAGAAA GAGATGGCCA CCATGAAGGA AGAATTCCAG 
AAAACCAAAG ATGAACTCGC CAAGTCAGAG GCAAAACGGA AGGAGCTAGA GGAAAAAATG 
GTCACTCTCT TAAAAGAGAA AAATGACCTG CAACTCCAGG TTCAATCTGA AGCAGATAGC 
TTGGCTGATG CAGAGGAAAG GTGTGAGCAA CTGATTAAAA ACAAAATCCA ACTTGAGGCC 
AAAATCAAAG AGGTGACTGA AAGAGCTGAG GAGGAGGAAG AGATCAATGC TGAGCTGACA 
GCCAAGAAGA GAAAACTGGA GGATGAATGT TCAGAACTCA AGAAAGACAT TGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACATGCCA CGGAGAACAA GGTGAAAAAT 
CTTACAGAAG AGATGGCAGG CCTGGATGAA ACCATTGCAA AACTGTCCAA GGAGAAGAAG 
GCTCTCCAAG AGACCCACCA GCAGACCCTG GATGACCTGC AGGCAGAGGA GGACAAAGTC 
AACATCCTGA CCAAAGCTAA AACCAAGCTA GAACAGCAAG TGGATGATCT TGAAGGGTCT 
CTGGAACAAG AAAAGAAGCT TCGAATGGAT CTAGAAAGAG CAAAGCGGAA ACTGGAGGGT 
GACCTCAAAT TGGCCCAAGA ATCCACAATG GATATGGAAA ATGACAAACA GCAACTTGAT 
GAAAAGCTTG AAAAGAAAGA ATTTGAAATC AGCAATTTGA TAAGCAAAAT TGAAGATGAG 
CAAGCTGTAG AAATTCAACT ACAGAAGAAG ATCAAAGAGT TGCAGGCCCG CATTGAGGAG 
CTGGGGGAAG AAATCGAGGC AGAGAGGGCG TCCCGAGCCA AAGCGGAGAA GCAGCGCTCT 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGAGGCTGG AAGAAGCCGG TGGGGCAACT 
TCTGCTCAGG TGGAATTGAA CAAGAAGCGG GAGGCTGAGT TTCAGAAACT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCATGAAGCT ATGGTGGCTG CTCTTCGGAA GAAGCACGCA 
GACAGTATGG CTGAGCTTGG GGAGCAGATT GACAACTTGC AGCGGGTCAA ACAGAAGCTG 
GAGAAGGAGA AGAGTGAGCT GAAGATGGAG ACTGATGACC TCAGCAGTAA CGCAGAGGCC 
ATTTCCAAAG CCAAGGGAAA CCTTGAAAAG ATGTGCCGCT CTCTAGAAGA TCAAGTGAGT 
GAGCTTAAGA CCAAGGAAGA GGAGCAGCAG CGGCTGATCA ATGACCTCAC AGCACAGAGA 
GCGCGCCTGC AGACAGAAGC GGGTGAATAT TCTCGACAAT TAGATGAGAA AGATGCTTTA 
GTCTCTCAGC TTTCAAGGAG CAAGCAAGCA TCTACTCAGC AGATTGAAGA GCTGAAACAT 
CAACTAGAGG AAGAAACTAA AGCCAAGAAC GCCCTGGCAC ACGCCCTGCA GTCCTCCCGC 
CATGACTGCG ACCTGCTGCG GGAACAGTAT GAGGAAGAGC AGGAAGGCAA AGCTGAGCTG 
CAGAGGGCGC TGTCCAAGGC CAACAGTGAG GTTGCCCAGT GGAGAACCAA ATACGAGACG 
GATGCCATCC AGCGCACAGA GGAGCTGGAG GAGGCCAAGA AAAAGTTGGC CCAGCGCCTG 
CAAGAAGCTG AGGAACATGT AGAAGCTGTG AACGCCAAAT GTGCTTCCCT TGAGAAGACG 
AAGCAGCGGC TCCAGAATGA AGTTGAAGAC CTCATGCTTG ATGTGGAAAG GTCTAATGCA 
GCCTGTGCAG CCCTTGATAA GAAGCAAAGG AACTTTGACA AGGTCCTATC AGAATGGAAG 
CAGAAGTATG AGGAAACTCA GGCTGAACTT GAGGCCTCCC AGAAGGAGTC ACGTTCTCTT 
AGCACTGAGC TGTTCAAGGT GAAGAATGTC TATGAGGAAT CCCTGGATCA ACTCGAAACG 
CTAAGAAGAG AAAATAAGAA CTTGCAACAG GAGATTTCTG ACCTCACTGA GCAGATTGCA 
GAGGGAGGAA AGCAAATTCA TGAATTGGAG AAAATAAAGA AGCAAGTAGA ACAAGAGAAA 
TGTGAAATTC AGGCTGCTTT AGAGGAAGCA GAGGCATCTC TTGAACATGA AGAAGGAAAG 
ATTCTGCGTA TCCAGCTTGA GTTAAACCAA GTCAAGTCTG AAGTTGATAG AAAAATCGCA 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGAAACCACA CTAGAGTCGT GGAGACAATG 
CAGAGCACGC TGGATGCAGA GATTAGAAGC AGAAATGATG CTCTGAGAGT CAAGAAGAAA 
ATGGAAGGAG ATCTGAATGA AATGGAAATC CAGCTGAACC ATGCCAATCG CTTAGCTGCA 
GAGAGTTTAA GGAACTACAG GAACACCCAA GGAATCCTGA AGGAAACCCA GCTCCACCTG 
GATGATGCTC TCCGGGGCCA GGAGGACCTC AAGGAACAGC TGGCAATTGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATCGAGGAG CTGTGGGCCA CTCTGGAACA GACAGAGAGA 
AGCAGGAAAA TCGCCGAACA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTCCAC 
ACCCAGAATA CCAGTCTCAT TAACACCAAG AAGAAATTAG AAAATGACGT TTCCCAACTC 
CAAAGTGAAG TGGAAGAAGT AATCCAAGAA TCACGCAATG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCTGCCAT GATGGCTGAG GAGCTGAAGA AGGAACAGGA CACCAGCGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCATCGTCTA 
GATGAGGCCG AGCAGCTGGC GCTGAAGGGT GGGAAGAAGC AGATCCAGAA ACTGGAGGCC 
AGGGTACGTG AGCTTGAAGG AGAGGTTGAA AATGAACAGA AACGTAATGC AGAGGCTGTT 
AAAGGTTTAC GGAAACATGA GCGACGAGTA AAAGAACTCA CCTACCAGAC TGAAGAAGAT 
CGCAAGAATG TTCTCAGGCT GCAGGACTTG GTAGATAAAT TACAGGCGAA GGTGAAATCA 
TACAAGAGAC AAGCTGAGGA GGCTGAGGAA CAATCCAATG CTAATCTATC TAAATTCCGC 
AAACTCCAGC ATGAGCTGGA GGAGGCCGAG GAACGGGCTG ACATTGCTGA GTCCCAGGTC 
AACAAATTGC GAGTGAAGAG CCGAGAGGTT CACACAAAAA TCAGTGCAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002463.2
CDS	96..5909
Translation	MSASSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVAEPKESYVKSTIQSKEGGKVTVKTEGGATLTVREDQVFPMNPPKYDKIEDMAMMTHLHEPGVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYKPEVVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKDESGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQITSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIDILGFTPEEKVSIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNAVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKSANFQKPKVVKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNDTVVGLYQKSAMKTLASLFSTYASAEADSSAKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQIITRTQAVCRGFLMRVEYQKMLQRREALFCIQYNVRAFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQSEADSLADAEERCEQLIKNKIQLEAKIKEVTERAEEEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLSKEKKALQETHQQTLDDLQAEEDKVNILTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESTMDMENDKQQLDEKLEKKEFEISNLISKIEDEQAVEIQLQKKIKELQARIEELGEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQVELNKKREAEFQKLRRDLEEATLQHEAMVAALRKKHADSMAELGEQIDNLQRVKQKLEKEKSELKMETDDLSSNAEAISKAKGNLEKMCRSLEDQVSELKTKEEEQQRLINDLTAQRARLQTEAGEYSRQLDEKDALVSQLSRSKQASTQQIEELKHQLEEETKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERSNAACAALDKKQRNFDKVLSEWKQKYEETQAELEASQKESRSLSTELFKVKNVYEESLDQLETLRRENKNLQQEISDLTEQIAEGGKQIHELEKIKKQVEQEKCEIQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQLKRNHTRVVETMQSTLDAEIRSRNDALRVKKKMEGDLNEMEIQLNHANRLAAESLRNYRNTQGILKETQLHLDDALRGQEDLKEQLAIVERRANLLQAEIEELWATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLENDVSQLQSEVEEVIQESRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVENEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQAKVKSYKRQAEEAEEQSNANLSKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKISAE

Target ORF information:

RefSeq Version	NM_002472.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 8 (MYH8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002472.2

ORF Insert Sequence:

ATGAGTGCGA GCTCAGACGC TGAGATGGCT GTTTTTGGCG AAGCTGCTCC CTACCTTCGA 
AAATCAGAAA AGGAGCGGAT TGAGGCCCAA AACAAGCCGT TTGATGCTAA AACATCTGTC 
TTTGTGGCGG AGCCCAAGGA ATCCTATGTG AAGAGCACTA TACAAAGCAA AGAAGGAGGG 
AAAGTAACCG TAAAGACTGA AGGTGGAGCA ACTCTAACTG TCAGGGAAGA CCAAGTCTTC 
CCTATGAACC CTCCGAAATA TGACAAAATT GAGGACATGG CCATGATGAC TCATCTACAC 
GAGCCTGGAG TGCTGTACAA CCTCAAAGAG CGCTATGCAG CCTGGATGAT CTACACCTAC 
TCAGGCCTCT TCTGTGTCAC CGTCAACCCC TACAAGTGGC TGCCGGTGTA CAAGCCCGAG 
GTGGTGGCTG CCTACAGAGG CAAAAAGCGC CAGGAGGCCC CGCCCCACAT CTTCTCCATC 
TCTGACAATG CCTATCAGTT CATGTTGACT GATCGAGAGA ATCAGTCCAT CCTGATCACC 
GGAGAATCTG GTGCCGGAAA GACTGTGAAC ACCAAGCGTG TCATCCAATA CTTTGCAACA 
ATTGCAGTTA CTGGAGAGAA GAAGAAGGAT GAATCTGGCA AAATGCAGGG GACTCTGGAA 
GATCAAATCA TCAGCGCCAA TCCCCTACTG GAGGCCTTTG GCAATGCCAA AACTGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATTAGAATCC ACTTTGGTAC TACAGGGAAG 
CTGGCATCTG CTGATATAGA AACATATCTT TTAGAAAAGT CCAGAGTTAC TTTCCAGCTA 
AAGGCGGAAA GAAGCTACCA TATTTTTTAT CAGATCACTT CCAATAAGAA GCCAGATCTA 
ATTGAAATGC TCCTGATCAC CACCAACCCA TATGACTATG CCTTCGTCAG TCAGGGGGAG 
ATCACAGTTC CCAGTATTGA TGACCAAGAA GAGTTGATGG CCACTGATAG TGCCATTGAC 
ATCCTGGGCT TCACTCCTGA AGAGAAAGTG TCCATCTATA AACTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCTGA GCCAGATGGC 
ACAGAAGTCG CTGACAAGGC AGCCTATCTC CAGAGTCTGA ACTCTGCAGA CCTACTCAAA 
GCCCTCTGCT ACCCTAGGGT CAAGGTTGGC AATGAGTATG TCACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAATGCGGT GGGTGCTCTG GCCAAAGCCG TCTACGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAAATC TTTGATTTTA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT TGTGCTAGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGACGTTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TTGAGCTCAT TGAGAAGCCA CTGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCAAC GGACACCTCC TTCAAGAACA AGCTGTATGA CCAGCACCTG 
GGCAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAAG GCAAGGCTGA GGCCCACTTC 
TCTCTGATTC ACTATGCTGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAAAAT 
AAGGACCCCC TGAATGATAC TGTGGTTGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTA 
GCCAGTCTCT TTTCCACGTA TGCTAGTGCT GAAGCAGATA GCAGCGCGAA GAAAGGTGCT 
AAGAAAAAGG GCTCTTCTTT CCAGACTGTG TCTGCCCTTT TCAGGGAAAA TTTAAATAAA 
TTGATGACGA ATCTGAGGAG CACACACCCT CACTTCGTAC GGTGTATCAT TCCCAATGAA 
ACCAAAACTC CTGGGGCAAT GGAACATGAA CTTGTGTTGC ACCAGCTGAG GTGTAATGGT 
GTGCTGGAAG GCATCCGCAT CTGTAGGAAA GGATTCCCAA GCAGAATCTT ATATGGTGAT 
TTCAAACAAA GATACAAGGT TTTAAATGCA AGTGCTATTC CAGAGGGACA GTTCATTGAC 
AGCAAGAAGG CTTCTGAGAA ACTTCTTGCA TCTATTGATA TTGATCATAC TCAATATAAA 
TTTGGACATA CCAAGGTTTT CTTCAAAGCT GGACTTCTGG GTCTTCTGGA AGAAATGAGA 
GATGAAAAAT TAGCCCAAAT TATAACAAGA ACACAAGCTG TCTGTAGGGG ATTCCTAATG 
AGGGTAGAAT ATCAGAAGAT GTTGCAAAGG AGAGAAGCAC TTTTCTGCAT CCAGTATAAT 
GTCCGTGCCT TCATGAACGT CAAGCACTGG CCCTGGATGA AACTCTTTTT CAAGATTAAG 
CCCCTCCTCA AGAGTGCAGA GACCGAGAAA GAGATGGCCA CCATGAAGGA AGAATTCCAG 
AAAACCAAAG ATGAACTCGC CAAGTCAGAG GCAAAACGGA AGGAGCTAGA GGAAAAAATG 
GTCACTCTCT TAAAAGAGAA AAATGACCTG CAACTCCAGG TTCAATCTGA AGCAGATAGC 
TTGGCTGATG CAGAGGAAAG GTGTGAGCAA CTGATTAAAA ACAAAATCCA ACTTGAGGCC 
AAAATCAAAG AGGTGACTGA AAGAGCTGAG GAGGAGGAAG AGATCAATGC TGAGCTGACA 
GCCAAGAAGA GAAAACTGGA GGATGAATGT TCAGAACTCA AGAAAGACAT TGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACATGCCA CGGAGAACAA GGTGAAAAAT 
CTTACAGAAG AGATGGCAGG CCTGGATGAA ACCATTGCAA AACTGTCCAA GGAGAAGAAG 
GCTCTCCAAG AGACCCACCA GCAGACCCTG GATGACCTGC AGGCAGAGGA GGACAAAGTC 
AACATCCTGA CCAAAGCTAA AACCAAGCTA GAACAGCAAG TGGATGATCT TGAAGGGTCT 
CTGGAACAAG AAAAGAAGCT TCGAATGGAT CTAGAAAGAG CAAAGCGGAA ACTGGAGGGT 
GACCTCAAAT TGGCCCAAGA ATCCACAATG GATATGGAAA ATGACAAACA GCAACTTGAT 
GAAAAGCTTG AAAAGAAAGA ATTTGAAATC AGCAATTTGA TAAGCAAAAT TGAAGATGAG 
CAAGCTGTAG AAATTCAACT ACAGAAGAAG ATCAAAGAGT TGCAGGCCCG CATTGAGGAG 
CTGGGGGAAG AAATCGAGGC AGAGAGGGCG TCCCGAGCCA AAGCGGAGAA GCAGCGCTCT 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGAGGCTGG AAGAAGCCGG TGGGGCAACT 
TCTGCTCAGG TGGAATTGAA CAAGAAGCGG GAGGCTGAGT TTCAGAAACT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCATGAAGCT ATGGTGGCTG CTCTTCGGAA GAAGCACGCA 
GACAGTATGG CTGAGCTTGG GGAGCAGATT GACAACTTGC AGCGGGTCAA ACAGAAGCTG 
GAGAAGGAGA AGAGTGAGCT GAAGATGGAG ACTGATGACC TCAGCAGTAA CGCAGAGGCC 
ATTTCCAAAG CCAAGGGAAA CCTTGAAAAG ATGTGCCGCT CTCTAGAAGA TCAAGTGAGT 
GAGCTTAAGA CCAAGGAAGA GGAGCAGCAG CGGCTGATCA ATGACCTCAC AGCACAGAGA 
GCGCGCCTGC AGACAGAAGC GGGTGAATAT TCTCGACAAT TAGATGAGAA AGATGCTTTA 
GTCTCTCAGC TTTCAAGGAG CAAGCAAGCA TCTACTCAGC AGATTGAAGA GCTGAAACAT 
CAACTAGAGG AAGAAACTAA AGCCAAGAAC GCCCTGGCAC ACGCCCTGCA GTCCTCCCGC 
CATGACTGCG ACCTGCTGCG GGAACAGTAT GAGGAAGAGC AGGAAGGCAA AGCTGAGCTG 
CAGAGGGCGC TGTCCAAGGC CAACAGTGAG GTTGCCCAGT GGAGAACCAA ATACGAGACG 
GATGCCATCC AGCGCACAGA GGAGCTGGAG GAGGCCAAGA AAAAGTTGGC CCAGCGCCTG 
CAAGAAGCTG AGGAACATGT AGAAGCTGTG AACGCCAAAT GTGCTTCCCT TGAGAAGACG 
AAGCAGCGGC TCCAGAATGA AGTTGAAGAC CTCATGCTTG ATGTGGAAAG GTCTAATGCA 
GCCTGTGCAG CCCTTGATAA GAAGCAAAGG AACTTTGACA AGGTCCTATC AGAATGGAAG 
CAGAAGTATG AGGAAACTCA GGCTGAACTT GAGGCCTCCC AGAAGGAGTC ACGTTCTCTT 
AGCACTGAGC TGTTCAAGGT GAAGAATGTC TATGAGGAAT CCCTGGATCA ACTCGAAACG 
CTAAGAAGAG AAAATAAGAA CTTGCAACAG GAGATTTCTG ACCTCACTGA GCAGATTGCA 
GAGGGAGGAA AGCAAATTCA TGAATTGGAG AAAATAAAGA AGCAAGTAGA ACAAGAGAAA 
TGTGAAATTC AGGCTGCTTT AGAGGAAGCA GAGGCATCTC TTGAACATGA AGAAGGAAAG 
ATTCTGCGTA TCCAGCTTGA GTTAAACCAA GTCAAGTCTG AAGTTGATAG AAAAATCGCA 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGAAACCACA CTAGAGTCGT GGAGACAATG 
CAGAGCACGC TGGATGCAGA GATTAGAAGC AGAAATGATG CTCTGAGAGT CAAGAAGAAA 
ATGGAAGGAG ATCTGAATGA AATGGAAATC CAGCTGAACC ATGCCAATCG CTTAGCTGCA 
GAGAGTTTAA GGAACTACAG GAACACCCAA GGAATCCTGA AGGAAACCCA GCTCCACCTG 
GATGATGCTC TCCGGGGCCA GGAGGACCTC AAGGAACAGC TGGCAATTGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATCGAGGAG CTGTGGGCCA CTCTGGAACA GACAGAGAGA 
AGCAGGAAAA TCGCCGAACA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTCCAC 
ACCCAGAATA CCAGTCTCAT TAACACCAAG AAGAAATTAG AAAATGACGT TTCCCAACTC 
CAAAGTGAAG TGGAAGAAGT AATCCAAGAA TCACGCAATG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCTGCCAT GATGGCTGAG GAGCTGAAGA AGGAACAGGA CACCAGCGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCATCGTCTA 
GATGAGGCCG AGCAGCTGGC GCTGAAGGGT GGGAAGAAGC AGATCCAGAA ACTGGAGGCC 
AGGGTACGTG AGCTTGAAGG AGAGGTTGAA AATGAACAGA AACGTAATGC AGAGGCTGTT 
AAAGGTTTAC GGAAACATGA GCGACGAGTA AAAGAACTCA CCTACCAGAC TGAAGAAGAT 
CGCAAGAATG TTCTCAGGCT GCAGGACTTG GTAGATAAAT TACAGGCGAA GGTGAAATCA 
TACAAGAGAC AAGCTGAGGA GGCTGAGGAA CAATCCAATG CTAATCTATC TAAATTCCGC 
AAACTCCAGC ATGAGCTGGA GGAGGCCGAG GAACGGGCTG ACATTGCTGA GTCCCAGGTC 
AACAAATTGC GAGTGAAGAG CCGAGAGGTT CACACAAAAA TCAGTGCAGA GTAA