DNAJB9 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNAJB9
Entrez Gene ID	4189
Full Name	DnaJ heat shock protein family (Hsp40) member B9
Synonyms	ERdj4,MDG-1,MDG1,MST049,MSTP049
General protein information	Preferred Names DnaJ heat shock protein family (Hsp40) member B9 Names dnaJ homolog subfamily B member 9 DnaJ (Hsp40) homolog, subfamily B, member 9 ER-resident protein ERdj4 endoplasmic reticulum DNA J domain-containing protein 4 endoplasmic reticulum DnaJ homolog 4 microvascular endothelial differentiation gene 1 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q31.1\|14q24.2-q24.3
Summary	This gene is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. This gene is a member of the type 2 subgroup of DnaJ proteins. The encoded protein is localized to the endoplasmic reticulum. This protein is induced by endoplasmic reticulum stress and plays a role in protecting stressed cells from apoptosis. [provided by RefSeq, Dec 2010].
Disorder MIM:	602634

mRNA and Protein(s)

mRNA	Protein	Name
NM_012328.3	NP_036460.1	dnaJ homolog subfamily B member 9 precursor
NM_012328.2	NP_036460.1	dnaJ homolog subfamily B member 9 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DNAJB9	XP_005628785.1
Bos taurus (cattle)	DNAJB9	NP_001179968.1
Rattus norvegicus (Norway rat)	Dnajb9	NP_036831.2
Gallus gallus (chicken)	DNAJB9	NP_001025906.1
Danio rerio (zebrafish)	dnajb9a	NP_001020355.1
Mus musculus (house mouse)	Dnajb9	NP_038788.2
Homo sapiens (human)	DNAJB9	NP_036460.1
Pan troglodytes (chimpanzee)	DNAJB9	XP_003951160.1
	DNAJB9	XP_001098110.1
Xenopus tropicalis (tropical clawed frog)	dnajb9	NP_001116196.1

Gene Ontology
Bibliography

Related articles in PubMed

A J-Protein Co-chaperone Recruits BiP to Monomerize IRE1 and Repress the Unfolded Protein Response.
Amin-Wetzel N, Saunders RA, Kamphuis MJ, Rato C, Preissler S, Harding HP, Ron D
Cell171(7)1625-1637.e13(2017 Dec)

Genotoxic stress/p53-induced DNAJB9 inhibits the pro-apoptotic function of p53.
Lee HJ, Kim JM, Kim KH, Heo JI, Kwak SJ, Han JA
Cell death and differentiation22(1)86-95(2015 Jan)

Large-scale genome-wide association study of Asian population reveals genetic factors in FRMD4A and other loci influencing smoking initiation and nicotine dependence.
Yoon D, Kim YJ, Cui WY, Van der Vaart A, Cho YS, Lee JY, Ma JZ, Payne TJ, Li MD, Park T
Human genetics131(6)1009-21(2012 Jun)

The diverse members of the mammalian HSP70 machine show distinct chaperone-like activities.
Hageman J, van Waarde MA, Zylicz A, Walerych D, Kampinga HH
The Biochemical journal435(1)127-42(2011 Apr)

Coxsackievirus B3 infection activates the unfolded protein response and induces apoptosis through downregulation of p58IPK and activation of CHOP and SREBP1.
Zhang HM, Ye X, Su Y, Yuan J, Liu Z, Stein DA, Yang D
Journal of virology84(17)8446-59(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study reports that the endoplasmic reticulum luminal co-chaperone ERdj4/DNAJB9 is a selective IRE1 repressor that promotes a complex between the luminal Hsp70 BiP and the luminal stress-sensing domain of IRE1alpha.
Title: A J-Protein Co-chaperone Recruits BiP to Monomerize IRE1 and Repress the Unfolded Protein Response.

these results demonstrate that DNAJB9 is a downstream target of p53 that belongs to the group of negative feedback regulators of p53.
Title: Genotoxic stress/p53-induced DNAJB9 inhibits the pro-apoptotic function of p53.

ERdj4 and ERdj5 promote turnover of misfolded SP-C and this activity is dependent on their ability to stimulate BiP ATPase activity.
Title: ERdj4 and ERdj5 are required for endoplasmic reticulum-associated protein degradation of misfolded surfactant protein C.

The following DNAJB9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNAJB9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32231	NM_012328.3 Latest version!	Homo sapiens DnaJ heat shock protein family (Hsp40) member B9 (DNAJB9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu32231	NM_012328.2	Homo sapiens DnaJ heat shock protein family (Hsp40) member B9 (DNAJB9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNAJB9 ( NM_012328.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036460.1 Homo sapiens DnaJ heat shock protein family (Hsp40) member B9 (DNAJB9), mRNA.

Price & Availability↑

CloneID	OHu32231
Clone ID Related Accession (Same CDS sequence)	NM_012328.3 , NM_012328.2
Accession Version	NM_012328.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily B member 9 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA202575.1 and AB026908.1. On Nov 23, 2018 this sequence version replaced NM_012328.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.178169.1, SRR3476690.1086571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000249356.4/ ENSP00000249356.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTACTC CCCAGTCAAT TTTCATCTTT GCAATCTGCA TTTTAATGAT AACAGAATTA 
ATTCTGGCCT CAAAAAGCTA CTATGATATC TTAGGTGTGC CAAAATCGGC ATCAGAGCGC 
CAAATCAAGA AGGCCTTTCA CAAGTTGGCC ATGAAGTACC ACCCTGACAA AAATAAGAGC 
CCGGATGCTG AAGCAAAATT CAGAGAGATT GCAGAAGCAT ATGAAACACT CTCAGATGCT 
AATAGACGAA AAGAGTATGA TACACTTGGA CACAGTGCTT TTACTAGTGG TAAAGGACAA 
AGAGGTAGTG GAAGTTCTTT TGAGCAGTCA TTTAACTTCA ATTTTGATGA CTTATTTAAA 
GACTTTGGCT TTTTTGGTCA AAACCAAAAC ACTGGATCCA AGAAGCGTTT TGAAAATCAT 
TTCCAGACAC GCCAGGATGG TGGTTCCAGT AGACAAAGGC ATCATTTCCA AGAATTTTCT 
TTTGGAGGTG GATTATTTGA TGACATGTTT GAAGATATGG AGAAAATGTT TTCTTTTAGT 
GGTTTTGACT CTACCAATCA GCATACAGTA CAGACTGAAA ATAGATTTCA TGGATCTAGC 
AAGCACTGCA GGACTGTCAC TCAACGAAGA GGAAATATGG TTACTACATA CACTGACTGT 
TCAGGACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036460.1
CDS	241..912
Translation	MATPQSIFIFAICILMITELILASKSYYDILGVPKSASERQIKKAFHKLAMKYHPDKNKSPDAEAKFREIAEAYETLSDANRRKEYDTLGHSAFTSGKGQRGSGSSFEQSFNFNFDDLFKDFGFFGQNQNTGSKKRFENHFQTRQDGGSSRQRHHFQEFSFGGGLFDDMFEDMEKMFSFSGFDSTNQHTVQTENRFHGSSKHCRTVTQRRGNMVTTYTDCSGQ

Target ORF information:

RefSeq Version	NM_012328.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member B9 (DNAJB9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012328.3

ORF Insert Sequence:

ATGGCTACTC CCCAGTCAAT TTTCATCTTT GCAATCTGCA TTTTAATGAT AACAGAATTA 
ATTCTGGCCT CAAAAAGCTA CTATGATATC TTAGGTGTGC CAAAATCGGC ATCAGAGCGC 
CAAATCAAGA AGGCCTTTCA CAAGTTGGCC ATGAAGTACC ACCCTGACAA AAATAAGAGC 
CCGGATGCTG AAGCAAAATT CAGAGAGATT GCAGAAGCAT ATGAAACACT CTCAGATGCT 
AATAGACGAA AAGAGTATGA TACACTTGGA CACAGTGCTT TTACTAGTGG TAAAGGACAA 
AGAGGTAGTG GAAGTTCTTT TGAGCAGTCA TTTAACTTCA ATTTTGATGA CTTATTTAAA 
GACTTTGGCT TTTTTGGTCA AAACCAAAAC ACTGGATCCA AGAAGCGTTT TGAAAATCAT 
TTCCAGACAC GCCAGGATGG TGGTTCCAGT AGACAAAGGC ATCATTTCCA AGAATTTTCT 
TTTGGAGGTG GATTATTTGA TGACATGTTT GAAGATATGG AGAAAATGTT TTCTTTTAGT 
GGTTTTGACT CTACCAATCA GCATACAGTA CAGACTGAAA ATAGATTTCA TGGATCTAGC 
AAGCACTGCA GGACTGTCAC TCAACGAAGA GGAAATATGG TTACTACATA CACTGACTGT 
TCAGGACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAJB9 ( NM_012328.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_036460.1 Homo sapiens DnaJ heat shock protein family (Hsp40) member B9 (DNAJB9), mRNA.

Price & Availability↑

CloneID	OHu32231
Clone ID Related Accession (Same CDS sequence)	NM_012328.3 , NM_012328.2
Accession Version	NM_012328.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily B member 9 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA202575.1 and AB026908.1. On Dec 3, 2010 this sequence version replaced NM_012328.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB026908.1, SRR1803611.69650.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTACTC CCCAGTCAAT TTTCATCTTT GCAATCTGCA TTTTAATGAT AACAGAATTA 
ATTCTGGCCT CAAAAAGCTA CTATGATATC TTAGGTGTGC CAAAATCGGC ATCAGAGCGC 
CAAATCAAGA AGGCCTTTCA CAAGTTGGCC ATGAAGTACC ACCCTGACAA AAATAAGAGC 
CCGGATGCTG AAGCAAAATT CAGAGAGATT GCAGAAGCAT ATGAAACACT CTCAGATGCT 
AATAGACGAA AAGAGTATGA TACACTTGGA CACAGTGCTT TTACTAGTGG TAAAGGACAA 
AGAGGTAGTG GAAGTTCTTT TGAGCAGTCA TTTAACTTCA ATTTTGATGA CTTATTTAAA 
GACTTTGGCT TTTTTGGTCA AAACCAAAAC ACTGGATCCA AGAAGCGTTT TGAAAATCAT 
TTCCAGACAC GCCAGGATGG TGGTTCCAGT AGACAAAGGC ATCATTTCCA AGAATTTTCT 
TTTGGAGGTG GATTATTTGA TGACATGTTT GAAGATATGG AGAAAATGTT TTCTTTTAGT 
GGTTTTGACT CTACCAATCA GCATACAGTA CAGACTGAAA ATAGATTTCA TGGATCTAGC 
AAGCACTGCA GGACTGTCAC TCAACGAAGA GGAAATATGG TTACTACATA CACTGACTGT 
TCAGGACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036460.1
CDS	370..1041
Translation	MATPQSIFIFAICILMITELILASKSYYDILGVPKSASERQIKKAFHKLAMKYHPDKNKSPDAEAKFREIAEAYETLSDANRRKEYDTLGHSAFTSGKGQRGSGSSFEQSFNFNFDDLFKDFGFFGQNQNTGSKKRFENHFQTRQDGGSSRQRHHFQEFSFGGGLFDDMFEDMEKMFSFSGFDSTNQHTVQTENRFHGSSKHCRTVTQRRGNMVTTYTDCSGQ

Target ORF information:

RefSeq Version	NM_012328.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member B9 (DNAJB9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012328.2

ORF Insert Sequence:

ATGGCTACTC CCCAGTCAAT TTTCATCTTT GCAATCTGCA TTTTAATGAT AACAGAATTA 
ATTCTGGCCT CAAAAAGCTA CTATGATATC TTAGGTGTGC CAAAATCGGC ATCAGAGCGC 
CAAATCAAGA AGGCCTTTCA CAAGTTGGCC ATGAAGTACC ACCCTGACAA AAATAAGAGC 
CCGGATGCTG AAGCAAAATT CAGAGAGATT GCAGAAGCAT ATGAAACACT CTCAGATGCT 
AATAGACGAA AAGAGTATGA TACACTTGGA CACAGTGCTT TTACTAGTGG TAAAGGACAA 
AGAGGTAGTG GAAGTTCTTT TGAGCAGTCA TTTAACTTCA ATTTTGATGA CTTATTTAAA 
GACTTTGGCT TTTTTGGTCA AAACCAAAAC ACTGGATCCA AGAAGCGTTT TGAAAATCAT 
TTCCAGACAC GCCAGGATGG TGGTTCCAGT AGACAAAGGC ATCATTTCCA AGAATTTTCT 
TTTGGAGGTG GATTATTTGA TGACATGTTT GAAGATATGG AGAAAATGTT TTCTTTTAGT 
GGTTTTGACT CTACCAATCA GCATACAGTA CAGACTGAAA ATAGATTTCA TGGATCTAGC 
AAGCACTGCA GGACTGTCAC TCAACGAAGA GGAAATATGG TTACTACATA CACTGACTGT 
TCAGGACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A J-Protein Co-chaperone Recruits BiP to Monomerize IRE1 and Repress the Unfolded Protein Response.
Cell171(7)1625-1637.e13(2017 Dec)
Amin-Wetzel N,Saunders RA,Kamphuis MJ,Rato C,Preissler S,Harding HP,Ron D

Genotoxic stress/p53-induced DNAJB9 inhibits the pro-apoptotic function of p53.
Cell death and differentiation22(1)86-95(2015 Jan)
Lee HJ,Kim JM,Kim KH,Heo JI,Kwak SJ,Han JA

Large-scale genome-wide association study of Asian population reveals genetic factors in FRMD4A and other loci influencing smoking initiation and nicotine dependence.
Human genetics131(6)1009-21(2012 Jun)
Yoon D,Kim YJ,Cui WY,Van der Vaart A,Cho YS,Lee JY,Ma JZ,Payne TJ,Li MD,Park T

The diverse members of the mammalian HSP70 machine show distinct chaperone-like activities.
The Biochemical journal435(1)127-42(2011 Apr)
Hageman J,van Waarde MA,Zylicz A,Walerych D,Kampinga HH

Coxsackievirus B3 infection activates the unfolded protein response and induces apoptosis through downregulation of p58IPK and activation of CHOP and SREBP1.
Journal of virology84(17)8446-59(2010 Sep)
Zhang HM,Ye X,Su Y,Yuan J,Liu Z,Stein DA,Yang D