POLN cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLN
Entrez Gene ID	353497
Full Name	DNA polymerase nu
Synonyms	POL4P
General protein information	Preferred Names DNA polymerase nu Names DNA polymerase nu DNA polymerase N DNA polymerase POL4P polymerase (DNA directed) nu polymerase (DNA) nu
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p16.3
Summary	This gene encodes a DNA polymerase type-A family member. The encoded protein plays a role in DNA repair and homologous recombination. This gene shares its 5' exons with some transcripts from overlapping GeneID: 79441, which encodes an augmentin-like protein complex subunit. [provided by RefSeq, Dec 2014].
Disorder MIM:	610887

mRNA and Protein(s)

mRNA	Protein	Name
NM_181808.4	NP_861524.2	DNA polymerase nu
NM_181808.3	NP_861524.2	DNA polymerase nu

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	poln	NP_001093496.1
Homo sapiens (human)	POLN	NP_861524.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic control of predominantly error-free replication through an acrolein-derived minor-groove DNA adduct.
Yoon JH, Hodge RP, Hackfeld LC, Park J, Roy Choudhury J, Prakash S, Prakash L
The Journal of biological chemistry(2018 Jan)

Mechanisms of Insertion of dCTP and dTTP Opposite the DNA Lesion O6-Methyl-2'-deoxyguanosine by Human DNA Polymerase ?.
Patra A, Zhang Q, Guengerich FP, Egli M
The Journal of biological chemistry291(46)24304-24313(2016 Nov)

Structural and Kinetic Analysis of Miscoding Opposite the DNA Adduct 1,N6-Ethenodeoxyadenosine by Human Translesion DNA Polymerase ?.
Patra A, Su Y, Zhang Q, Johnson KM, Guengerich FP, Egli M
The Journal of biological chemistry291(27)14134-45(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The structural data are consistent with the observed tendency of hpol eta to insert both dC and dT opposite the O(6)-MeG lesion with similar efficiencies.
Title: Mechanisms of Insertion of dCTP and dTTP Opposite the DNA Lesion O6-Methyl-2'-deoxyguanosine by Human DNA Polymerase ?.

Five x-ray crystal structures of hpol eta ternary complexes were determined, three at the insertion and two at the extension stage.
Title: Structural and Kinetic Analysis of Miscoding Opposite the DNA Adduct 1,N6-Ethenodeoxyadenosine by Human Translesion DNA Polymerase ?.

The Proliferating Cell Nuclear Antigen (PCNA)-interacting Protein (PIP) Motif of DNA Polymerase eta Mediates Its Interaction with the C-terminal Domain of Rev1.
Title: The Proliferating Cell Nuclear Antigen (PCNA)-interacting Protein (PIP) Motif of DNA Polymerase ? Mediates Its Interaction with the C-terminal Domain of Rev1.

This is the first crystal structure of a DNA polymerase with an incoming rNTP opposite a DNA lesion.
Title: Mechanism of Ribonucleotide Incorporation by Human DNA Polymerase ?.

Pol nu catalyzes both correct and mispair formation with high catalytic efficiency.
Title: Human DNA Polymerase ? Catalyzes Correct and Incorrect DNA Synthesis with High Catalytic Efficiency.

The following POLN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30074	NM_181808.4 Latest version!	Homo sapiens DNA polymerase nu (POLN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OHu30074	NM_181808.3	Homo sapiens DNA polymerase nu (POLN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLN ( NM_181808.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_861524.2 Homo sapiens DNA polymerase nu (POLN), mRNA.

Price & Availability↑

CloneID	OHu30074
Clone ID Related Accession (Same CDS sequence)	NM_181808.4 , NM_181808.3
Accession Version	NM_181808.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	DNA polymerase nu
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB098342.1, AK303673.1, AY136549.1, AL122048.1 and AL136360.21. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_181808.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY136549.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology MANE Ensembl match :: ENST00000511885.6/ ENSP00000435506.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAAATT ATGAGGCATT GGTAGGCTTT GATCTCTGTA ATACACCGCT CTCCAGTGTT 
GCTCAGAAGA TTATGTCTGC TATGCATTCA GGTGATTTAG TGGATTCTAA GACTTGGGGA 
AAGAGTACAG AGACTATGGA AGTGATAAAC AAGTCCAGTG TTAAGTATTC AGTACAACTT 
GAAGACAGGA AGACTCAATC ACCAGAAAAA AAGGATCTTA AATCTTTAAG AAGTCAGACA 
TCAAGAGGTT CTGCCAAGCT GTCTCCTCAG TCCTTCAGTG TCAGGCTCAC AGATCAGCTG 
TCTGCTGACC AAAAACAGAA GAGCATCAGC TCATTGACTC TTTCAAGTTG TTTAATTCCA 
CAGTATAATC AAGAGGCTTC AGTTCTACAG AAAAAGGGGC ATAAAAGAAA GCATTTCCTA 
ATGGAGAATA TAAATAATGA AAATAAAGGA AGCATTAATC TTAAAAGAAA ACATATTACA 
TATAATAATT TGTCAGAGAA AACAAGTAAA CAAATGGCAT TGGAAGAAGA TACTGATGAC 
GCCGAAGGCT ACCTAAATTC TGGGAACTCA GGAGCATTGA AAAAACATTT TTGTGATATT 
AGGCATTTGG ATGATTGGGC AAAAAGCCAG CTGATTGAAA TGCTCAAACA GGCAGCAGCC 
CTGGTGATAA CTGTGATGTA TACTGATGGT TCCACCCAGC TAGGAGCTGA CCAGACCCCC 
GTTTCTTCTG TTAGAGGAAT TGTGGTGTTA GTAAAACGCC AAGCAGAGGG TGGCCATGGC 
TGTCCAGATG CCCCGGCCTG TGGTCCTGTT CTGGAGGGCT TTGTGTCAGA TGATCCATGC 
ATCTACATTC AAATAGAGCA CTCTGCTATC TGGGACCAAG AACAGGAGGC ACATCAACAA 
TTTGCCCGGA ACGTGCTATT TCAAACAATG AAATGTAAAT GTCCTGTTAT TTGTTTTAAT 
GCTAAGGATT TTGTGAGAAT AGTGCTGCAG TTTTTTGGCA ATGATGGCAG TTGGAAGCAT 
GTTGCTGATT TTATAGGGCT AGATCCCAGA ATTGCTGCAT GGCTTATAGA TCCTAGTGAT 
GCCACACCCT CTTTTGAAGA TTTAGTAGAA AAATACTGTG AAAAATCCAT TACAGTTAAA 
GTGAACAGCA CATATGGAAA TTCCTCAAGA AATATTGTGA ATCAGAATGT ACGTGAGAAC 
CTGAAGACAC TCTACAGACT TACAATGGAC CTTTGCTCTA AACTGAAGGA TTATGGTTTA 
TGGCAACTAT TTCGTACTTT GGAGCTTCCT CTGATACCAA TTTTGGCAGT GATGGAAAGC 
CATGCCATTC AGGTGAACAA AGAGGAGATG GAGAAGACGT CAGCACTTCT TGGGGCTCGT 
CTCAAGGAAT TGGAGCAAGA AGCTCATTTT GTTGCAGGAG AACGGTTTCT TATAACGAGC 
AATAACCAGC TTCGAGAGAT CCTCTTTGGC AAGTTAAAGC TGCACCTGCT GAGTCAAAGG 
AACAGTCTCC CCAGAACGGG GTTGCAGAAA TACCCGTCTA CATCAGAAGC AGTGTTAAAT 
GCTCTGCGAG ACCTTCATCC ATTACCCAAG ATAATTTTGG AATACAGGCA GGTTCACAAG 
ATCAAGTCAA CCTTTGTAGA TGGATTACTA GCTTGCATGA AAAAGGGCTC CATTTCCTCT 
ACATGGAATC AGACTGGAAC TGTGACTGGA AGACTTTCAG CCAAGCATCC TAATATCCAA 
GGTATCTCCA AGCACCCAAT TCAGATTACT ACACCTAAGA ATTTTAAAGG TAAAGAAGAC 
AAGATTCTCA CGATCTCCCC GAGGGCCATG TTTGTTTCAT CCAAAGGCCA CACCTTTCTA 
GCAGCAGACT TTTCACAGAT TGAATTGCGC ATTCTTACAC ATTTATCTGG AGATCCGGAA 
CTTCTGAAGT TATTCCAGGA ATCTGAAAGA GATGATGTAT TTTCTACTCT GACTTCACAG 
TGGAAGGATG TGCCCGTGGA ACAGGTGACA CACGCAGACA GAGAGCAAAC CAAGAAGGTG 
GTGTACGCGG TGGTCTATGG AGCAGGGAAG GAGCGGCTGG CTGCTTGCCT TGGAGTTCCT 
ATTCAGGAAG CTGCCCAGTT TTTGGAGAGT TTTTTGCAGA AGTACAAGAA AATCAAGGAC 
TTCGCCCGAG CAGCTATTGC CCAGTGTCAC CAGACAGGCT GTGTGGTGTC CATCATGGGC 
AGAAGGAGAC CCCTGCCAAG GATTCACGCT CATGACCAGC AACTCCGGGC ACAAGCAGAG 
CGACAGGCAG TGAACTTCGT GGTGCAAGGC TCCGCTGCTG ACCTCTGCAA GCTGGCCATG 
ATCCATGTCT TCACTGCAGT GGCTGCTTCC CACACCTTGA CGGCCAGGCT GGTGGCCCAG 
ATCCATGATG AGCTGCTGTT TGAAGTGGAA GATCCGCAGA TCCCGGAGTG TGCAGCTCTC 
GTCAGGAGGA CCATGGAGTC CTTGGAACAG GTGCAGGCAT TGGAGCTGCA GCTTCAGGTA 
CCCCTCAAGG TGAGCCTGAG TGCCGGCCGC TCATGGGGAC ACCTGGTGCC ACTGCAGGAG 
GCCTGGGGCC CTCCGCCAGG CCCATGTCGC ACTGAGTCTC CCAGCAACAG CCTGGCTGCC 
CCTGGGTCCC CTGCCAGCAC CCAGCCCCCA CCCCTGCATT TTTCGCCTTC ATTTTGTCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_861524.2
CDS	355..3057
Translation	MENYEALVGFDLCNTPLSSVAQKIMSAMHSGDLVDSKTWGKSTETMEVINKSSVKYSVQLEDRKTQSPEKKDLKSLRSQTSRGSAKLSPQSFSVRLTDQLSADQKQKSISSLTLSSCLIPQYNQEASVLQKKGHKRKHFLMENINNENKGSINLKRKHITYNNLSEKTSKQMALEEDTDDAEGYLNSGNSGALKKHFCDIRHLDDWAKSQLIEMLKQAAALVITVMYTDGSTQLGADQTPVSSVRGIVVLVKRQAEGGHGCPDAPACGPVLEGFVSDDPCIYIQIEHSAIWDQEQEAHQQFARNVLFQTMKCKCPVICFNAKDFVRIVLQFFGNDGSWKHVADFIGLDPRIAAWLIDPSDATPSFEDLVEKYCEKSITVKVNSTYGNSSRNIVNQNVRENLKTLYRLTMDLCSKLKDYGLWQLFRTLELPLIPILAVMESHAIQVNKEEMEKTSALLGARLKELEQEAHFVAGERFLITSNNQLREILFGKLKLHLLSQRNSLPRTGLQKYPSTSEAVLNALRDLHPLPKIILEYRQVHKIKSTFVDGLLACMKKGSISSTWNQTGTVTGRLSAKHPNIQGISKHPIQITTPKNFKGKEDKILTISPRAMFVSSKGHTFLAADFSQIELRILTHLSGDPELLKLFQESERDDVFSTLTSQWKDVPVEQVTHADREQTKKVVYAVVYGAGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAAIAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIPECAALVRRTMESLEQVQALELQLQVPLKVSLSAGRSWGHLVPLQEAWGPPPGPCRTESPSNSLAAPGSPASTQPPPLHFSPSFCL

Target ORF information:

RefSeq Version	NM_181808.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase nu (POLN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181808.4

ORF Insert Sequence:

ATGGAAAATT ATGAGGCATT GGTAGGCTTT GATCTCTGTA ATACACCGCT CTCCAGTGTT 
GCTCAGAAGA TTATGTCTGC TATGCATTCA GGTGATTTAG TGGATTCTAA GACTTGGGGA 
AAGAGTACAG AGACTATGGA AGTGATAAAC AAGTCCAGTG TTAAGTATTC AGTACAACTT 
GAAGACAGGA AGACTCAATC ACCAGAAAAA AAGGATCTTA AATCTTTAAG AAGTCAGACA 
TCAAGAGGTT CTGCCAAGCT GTCTCCTCAG TCCTTCAGTG TCAGGCTCAC AGATCAGCTG 
TCTGCTGACC AAAAACAGAA GAGCATCAGC TCATTGACTC TTTCAAGTTG TTTAATTCCA 
CAGTATAATC AAGAGGCTTC AGTTCTACAG AAAAAGGGGC ATAAAAGAAA GCATTTCCTA 
ATGGAGAATA TAAATAATGA AAATAAAGGA AGCATTAATC TTAAAAGAAA ACATATTACA 
TATAATAATT TGTCAGAGAA AACAAGTAAA CAAATGGCAT TGGAAGAAGA TACTGATGAC 
GCCGAAGGCT ACCTAAATTC TGGGAACTCA GGAGCATTGA AAAAACATTT TTGTGATATT 
AGGCATTTGG ATGATTGGGC AAAAAGCCAG CTGATTGAAA TGCTCAAACA GGCAGCAGCC 
CTGGTGATAA CTGTGATGTA TACTGATGGT TCCACCCAGC TAGGAGCTGA CCAGACCCCC 
GTTTCTTCTG TTAGAGGAAT TGTGGTGTTA GTAAAACGCC AAGCAGAGGG TGGCCATGGC 
TGTCCAGATG CCCCGGCCTG TGGTCCTGTT CTGGAGGGCT TTGTGTCAGA TGATCCATGC 
ATCTACATTC AAATAGAGCA CTCTGCTATC TGGGACCAAG AACAGGAGGC ACATCAACAA 
TTTGCCCGGA ACGTGCTATT TCAAACAATG AAATGTAAAT GTCCTGTTAT TTGTTTTAAT 
GCTAAGGATT TTGTGAGAAT AGTGCTGCAG TTTTTTGGCA ATGATGGCAG TTGGAAGCAT 
GTTGCTGATT TTATAGGGCT AGATCCCAGA ATTGCTGCAT GGCTTATAGA TCCTAGTGAT 
GCCACACCCT CTTTTGAAGA TTTAGTAGAA AAATACTGTG AAAAATCCAT TACAGTTAAA 
GTGAACAGCA CATATGGAAA TTCCTCAAGA AATATTGTGA ATCAGAATGT ACGTGAGAAC 
CTGAAGACAC TCTACAGACT TACAATGGAC CTTTGCTCTA AACTGAAGGA TTATGGTTTA 
TGGCAACTAT TTCGTACTTT GGAGCTTCCT CTGATACCAA TTTTGGCAGT GATGGAAAGC 
CATGCCATTC AGGTGAACAA AGAGGAGATG GAGAAGACGT CAGCACTTCT TGGGGCTCGT 
CTCAAGGAAT TGGAGCAAGA AGCTCATTTT GTTGCAGGAG AACGGTTTCT TATAACGAGC 
AATAACCAGC TTCGAGAGAT CCTCTTTGGC AAGTTAAAGC TGCACCTGCT GAGTCAAAGG 
AACAGTCTCC CCAGAACGGG GTTGCAGAAA TACCCGTCTA CATCAGAAGC AGTGTTAAAT 
GCTCTGCGAG ACCTTCATCC ATTACCCAAG ATAATTTTGG AATACAGGCA GGTTCACAAG 
ATCAAGTCAA CCTTTGTAGA TGGATTACTA GCTTGCATGA AAAAGGGCTC CATTTCCTCT 
ACATGGAATC AGACTGGAAC TGTGACTGGA AGACTTTCAG CCAAGCATCC TAATATCCAA 
GGTATCTCCA AGCACCCAAT TCAGATTACT ACACCTAAGA ATTTTAAAGG TAAAGAAGAC 
AAGATTCTCA CGATCTCCCC GAGGGCCATG TTTGTTTCAT CCAAAGGCCA CACCTTTCTA 
GCAGCAGACT TTTCACAGAT TGAATTGCGC ATTCTTACAC ATTTATCTGG AGATCCGGAA 
CTTCTGAAGT TATTCCAGGA ATCTGAAAGA GATGATGTAT TTTCTACTCT GACTTCACAG 
TGGAAGGATG TGCCCGTGGA ACAGGTGACA CACGCAGACA GAGAGCAAAC CAAGAAGGTG 
GTGTACGCGG TGGTCTATGG AGCAGGGAAG GAGCGGCTGG CTGCTTGCCT TGGAGTTCCT 
ATTCAGGAAG CTGCCCAGTT TTTGGAGAGT TTTTTGCAGA AGTACAAGAA AATCAAGGAC 
TTCGCCCGAG CAGCTATTGC CCAGTGTCAC CAGACAGGCT GTGTGGTGTC CATCATGGGC 
AGAAGGAGAC CCCTGCCAAG GATTCACGCT CATGACCAGC AACTCCGGGC ACAAGCAGAG 
CGACAGGCAG TGAACTTCGT GGTGCAAGGC TCCGCTGCTG ACCTCTGCAA GCTGGCCATG 
ATCCATGTCT TCACTGCAGT GGCTGCTTCC CACACCTTGA CGGCCAGGCT GGTGGCCCAG 
ATCCATGATG AGCTGCTGTT TGAAGTGGAA GATCCGCAGA TCCCGGAGTG TGCAGCTCTC 
GTCAGGAGGA CCATGGAGTC CTTGGAACAG GTGCAGGCAT TGGAGCTGCA GCTTCAGGTA 
CCCCTCAAGG TGAGCCTGAG TGCCGGCCGC TCATGGGGAC ACCTGGTGCC ACTGCAGGAG 
GCCTGGGGCC CTCCGCCAGG CCCATGTCGC ACTGAGTCTC CCAGCAACAG CCTGGCTGCC 
CCTGGGTCCC CTGCCAGCAC CCAGCCCCCA CCCCTGCATT TTTCGCCTTC ATTTTGTCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLN ( NM_181808.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_861524.2 Homo sapiens DNA polymerase nu (POLN), mRNA.

Price & Availability↑

CloneID	OHu30074
Clone ID Related Accession (Same CDS sequence)	NM_181808.4 , NM_181808.3
Accession Version	NM_181808.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	DNA polymerase nu
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY010559.1, DB098342.1, AK303673.1, AY136549.1 and AL122048.1. This sequence is a reference standard in the RefSeqGene project. On Dec 19, 2014 this sequence version replaced NM_181808.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY136549.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAAATT ATGAGGCATT GGTAGGCTTT GATCTCTGTA ATACACCGCT CTCCAGTGTT 
GCTCAGAAGA TTATGTCTGC TATGCATTCA GGTGATTTAG TGGATTCTAA GACTTGGGGA 
AAGAGTACAG AGACTATGGA AGTGATAAAC AAGTCCAGTG TTAAGTATTC AGTACAACTT 
GAAGACAGGA AGACTCAATC ACCAGAAAAA AAGGATCTTA AATCTTTAAG AAGTCAGACA 
TCAAGAGGTT CTGCCAAGCT GTCTCCTCAG TCCTTCAGTG TCAGGCTCAC AGATCAGCTG 
TCTGCTGACC AAAAACAGAA GAGCATCAGC TCATTGACTC TTTCAAGTTG TTTAATTCCA 
CAGTATAATC AAGAGGCTTC AGTTCTACAG AAAAAGGGGC ATAAAAGAAA GCATTTCCTA 
ATGGAGAATA TAAATAATGA AAATAAAGGA AGCATTAATC TTAAAAGAAA ACATATTACA 
TATAATAATT TGTCAGAGAA AACAAGTAAA CAAATGGCAT TGGAAGAAGA TACTGATGAC 
GCCGAAGGCT ACCTAAATTC TGGGAACTCA GGAGCATTGA AAAAACATTT TTGTGATATT 
AGGCATTTGG ATGATTGGGC AAAAAGCCAG CTGATTGAAA TGCTCAAACA GGCAGCAGCC 
CTGGTGATAA CTGTGATGTA TACTGATGGT TCCACCCAGC TAGGAGCTGA CCAGACCCCC 
GTTTCTTCTG TTAGAGGAAT TGTGGTGTTA GTAAAACGCC AAGCAGAGGG TGGCCATGGC 
TGTCCAGATG CCCCGGCCTG TGGTCCTGTT CTGGAGGGCT TTGTGTCAGA TGATCCATGC 
ATCTACATTC AAATAGAGCA CTCTGCTATC TGGGACCAAG AACAGGAGGC ACATCAACAA 
TTTGCCCGGA ACGTGCTATT TCAAACAATG AAATGTAAAT GTCCTGTTAT TTGTTTTAAT 
GCTAAGGATT TTGTGAGAAT AGTGCTGCAG TTTTTTGGCA ATGATGGCAG TTGGAAGCAT 
GTTGCTGATT TTATAGGGCT AGATCCCAGA ATTGCTGCAT GGCTTATAGA TCCTAGTGAT 
GCCACACCCT CTTTTGAAGA TTTAGTAGAA AAATACTGTG AAAAATCCAT TACAGTTAAA 
GTGAACAGCA CATATGGAAA TTCCTCAAGA AATATTGTGA ATCAGAATGT ACGTGAGAAC 
CTGAAGACAC TCTACAGACT TACAATGGAC CTTTGCTCTA AACTGAAGGA TTATGGTTTA 
TGGCAACTAT TTCGTACTTT GGAGCTTCCT CTGATACCAA TTTTGGCAGT GATGGAAAGC 
CATGCCATTC AGGTGAACAA AGAGGAGATG GAGAAGACGT CAGCACTTCT TGGGGCTCGT 
CTCAAGGAAT TGGAGCAAGA AGCTCATTTT GTTGCAGGAG AACGGTTTCT TATAACGAGC 
AATAACCAGC TTCGAGAGAT CCTCTTTGGC AAGTTAAAGC TGCACCTGCT GAGTCAAAGG 
AACAGTCTCC CCAGAACGGG GTTGCAGAAA TACCCGTCTA CATCAGAAGC AGTGTTAAAT 
GCTCTGCGAG ACCTTCATCC ATTACCCAAG ATAATTTTGG AATACAGGCA GGTTCACAAG 
ATCAAGTCAA CCTTTGTAGA TGGATTACTA GCTTGCATGA AAAAGGGCTC CATTTCCTCT 
ACATGGAATC AGACTGGAAC TGTGACTGGA AGACTTTCAG CCAAGCATCC TAATATCCAA 
GGTATCTCCA AGCACCCAAT TCAGATTACT ACACCTAAGA ATTTTAAAGG TAAAGAAGAC 
AAGATTCTCA CGATCTCCCC GAGGGCCATG TTTGTTTCAT CCAAAGGCCA CACCTTTCTA 
GCAGCAGACT TTTCACAGAT TGAATTGCGC ATTCTTACAC ATTTATCTGG AGATCCGGAA 
CTTCTGAAGT TATTCCAGGA ATCTGAAAGA GATGATGTAT TTTCTACTCT GACTTCACAG 
TGGAAGGATG TGCCCGTGGA ACAGGTGACA CACGCAGACA GAGAGCAAAC CAAGAAGGTG 
GTGTACGCGG TGGTCTATGG AGCAGGGAAG GAGCGGCTGG CTGCTTGCCT TGGAGTTCCT 
ATTCAGGAAG CTGCCCAGTT TTTGGAGAGT TTTTTGCAGA AGTACAAGAA AATCAAGGAC 
TTCGCCCGAG CAGCTATTGC CCAGTGTCAC CAGACAGGCT GTGTGGTGTC CATCATGGGC 
AGAAGGAGAC CCCTGCCAAG GATTCACGCT CATGACCAGC AACTCCGGGC ACAAGCAGAG 
CGACAGGCAG TGAACTTCGT GGTGCAAGGC TCCGCTGCTG ACCTCTGCAA GCTGGCCATG 
ATCCATGTCT TCACTGCAGT GGCTGCTTCC CACACCTTGA CGGCCAGGCT GGTGGCCCAG 
ATCCATGATG AGCTGCTGTT TGAAGTGGAA GATCCGCAGA TCCCGGAGTG TGCAGCTCTC 
GTCAGGAGGA CCATGGAGTC CTTGGAACAG GTGCAGGCAT TGGAGCTGCA GCTTCAGGTA 
CCCCTCAAGG TGAGCCTGAG TGCCGGCCGC TCATGGGGAC ACCTGGTGCC ACTGCAGGAG 
GCCTGGGGCC CTCCGCCAGG CCCATGTCGC ACTGAGTCTC CCAGCAACAG CCTGGCTGCC 
CCTGGGTCCC CTGCCAGCAC CCAGCCCCCA CCCCTGCATT TTTCGCCTTC ATTTTGTCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_861524.2
CDS	398..3100
Translation	MENYEALVGFDLCNTPLSSVAQKIMSAMHSGDLVDSKTWGKSTETMEVINKSSVKYSVQLEDRKTQSPEKKDLKSLRSQTSRGSAKLSPQSFSVRLTDQLSADQKQKSISSLTLSSCLIPQYNQEASVLQKKGHKRKHFLMENINNENKGSINLKRKHITYNNLSEKTSKQMALEEDTDDAEGYLNSGNSGALKKHFCDIRHLDDWAKSQLIEMLKQAAALVITVMYTDGSTQLGADQTPVSSVRGIVVLVKRQAEGGHGCPDAPACGPVLEGFVSDDPCIYIQIEHSAIWDQEQEAHQQFARNVLFQTMKCKCPVICFNAKDFVRIVLQFFGNDGSWKHVADFIGLDPRIAAWLIDPSDATPSFEDLVEKYCEKSITVKVNSTYGNSSRNIVNQNVRENLKTLYRLTMDLCSKLKDYGLWQLFRTLELPLIPILAVMESHAIQVNKEEMEKTSALLGARLKELEQEAHFVAGERFLITSNNQLREILFGKLKLHLLSQRNSLPRTGLQKYPSTSEAVLNALRDLHPLPKIILEYRQVHKIKSTFVDGLLACMKKGSISSTWNQTGTVTGRLSAKHPNIQGISKHPIQITTPKNFKGKEDKILTISPRAMFVSSKGHTFLAADFSQIELRILTHLSGDPELLKLFQESERDDVFSTLTSQWKDVPVEQVTHADREQTKKVVYAVVYGAGKERLAACLGVPIQEAAQFLESFLQKYKKIKDFARAAIAQCHQTGCVVSIMGRRRPLPRIHAHDQQLRAQAERQAVNFVVQGSAADLCKLAMIHVFTAVAASHTLTARLVAQIHDELLFEVEDPQIPECAALVRRTMESLEQVQALELQLQVPLKVSLSAGRSWGHLVPLQEAWGPPPGPCRTESPSNSLAAPGSPASTQPPPLHFSPSFCL

Target ORF information:

RefSeq Version	NM_181808.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase nu (POLN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181808.3

ORF Insert Sequence:

ATGGAAAATT ATGAGGCATT GGTAGGCTTT GATCTCTGTA ATACACCGCT CTCCAGTGTT 
GCTCAGAAGA TTATGTCTGC TATGCATTCA GGTGATTTAG TGGATTCTAA GACTTGGGGA 
AAGAGTACAG AGACTATGGA AGTGATAAAC AAGTCCAGTG TTAAGTATTC AGTACAACTT 
GAAGACAGGA AGACTCAATC ACCAGAAAAA AAGGATCTTA AATCTTTAAG AAGTCAGACA 
TCAAGAGGTT CTGCCAAGCT GTCTCCTCAG TCCTTCAGTG TCAGGCTCAC AGATCAGCTG 
TCTGCTGACC AAAAACAGAA GAGCATCAGC TCATTGACTC TTTCAAGTTG TTTAATTCCA 
CAGTATAATC AAGAGGCTTC AGTTCTACAG AAAAAGGGGC ATAAAAGAAA GCATTTCCTA 
ATGGAGAATA TAAATAATGA AAATAAAGGA AGCATTAATC TTAAAAGAAA ACATATTACA 
TATAATAATT TGTCAGAGAA AACAAGTAAA CAAATGGCAT TGGAAGAAGA TACTGATGAC 
GCCGAAGGCT ACCTAAATTC TGGGAACTCA GGAGCATTGA AAAAACATTT TTGTGATATT 
AGGCATTTGG ATGATTGGGC AAAAAGCCAG CTGATTGAAA TGCTCAAACA GGCAGCAGCC 
CTGGTGATAA CTGTGATGTA TACTGATGGT TCCACCCAGC TAGGAGCTGA CCAGACCCCC 
GTTTCTTCTG TTAGAGGAAT TGTGGTGTTA GTAAAACGCC AAGCAGAGGG TGGCCATGGC 
TGTCCAGATG CCCCGGCCTG TGGTCCTGTT CTGGAGGGCT TTGTGTCAGA TGATCCATGC 
ATCTACATTC AAATAGAGCA CTCTGCTATC TGGGACCAAG AACAGGAGGC ACATCAACAA 
TTTGCCCGGA ACGTGCTATT TCAAACAATG AAATGTAAAT GTCCTGTTAT TTGTTTTAAT 
GCTAAGGATT TTGTGAGAAT AGTGCTGCAG TTTTTTGGCA ATGATGGCAG TTGGAAGCAT 
GTTGCTGATT TTATAGGGCT AGATCCCAGA ATTGCTGCAT GGCTTATAGA TCCTAGTGAT 
GCCACACCCT CTTTTGAAGA TTTAGTAGAA AAATACTGTG AAAAATCCAT TACAGTTAAA 
GTGAACAGCA CATATGGAAA TTCCTCAAGA AATATTGTGA ATCAGAATGT ACGTGAGAAC 
CTGAAGACAC TCTACAGACT TACAATGGAC CTTTGCTCTA AACTGAAGGA TTATGGTTTA 
TGGCAACTAT TTCGTACTTT GGAGCTTCCT CTGATACCAA TTTTGGCAGT GATGGAAAGC 
CATGCCATTC AGGTGAACAA AGAGGAGATG GAGAAGACGT CAGCACTTCT TGGGGCTCGT 
CTCAAGGAAT TGGAGCAAGA AGCTCATTTT GTTGCAGGAG AACGGTTTCT TATAACGAGC 
AATAACCAGC TTCGAGAGAT CCTCTTTGGC AAGTTAAAGC TGCACCTGCT GAGTCAAAGG 
AACAGTCTCC CCAGAACGGG GTTGCAGAAA TACCCGTCTA CATCAGAAGC AGTGTTAAAT 
GCTCTGCGAG ACCTTCATCC ATTACCCAAG ATAATTTTGG AATACAGGCA GGTTCACAAG 
ATCAAGTCAA CCTTTGTAGA TGGATTACTA GCTTGCATGA AAAAGGGCTC CATTTCCTCT 
ACATGGAATC AGACTGGAAC TGTGACTGGA AGACTTTCAG CCAAGCATCC TAATATCCAA 
GGTATCTCCA AGCACCCAAT TCAGATTACT ACACCTAAGA ATTTTAAAGG TAAAGAAGAC 
AAGATTCTCA CGATCTCCCC GAGGGCCATG TTTGTTTCAT CCAAAGGCCA CACCTTTCTA 
GCAGCAGACT TTTCACAGAT TGAATTGCGC ATTCTTACAC ATTTATCTGG AGATCCGGAA 
CTTCTGAAGT TATTCCAGGA ATCTGAAAGA GATGATGTAT TTTCTACTCT GACTTCACAG 
TGGAAGGATG TGCCCGTGGA ACAGGTGACA CACGCAGACA GAGAGCAAAC CAAGAAGGTG 
GTGTACGCGG TGGTCTATGG AGCAGGGAAG GAGCGGCTGG CTGCTTGCCT TGGAGTTCCT 
ATTCAGGAAG CTGCCCAGTT TTTGGAGAGT TTTTTGCAGA AGTACAAGAA AATCAAGGAC 
TTCGCCCGAG CAGCTATTGC CCAGTGTCAC CAGACAGGCT GTGTGGTGTC CATCATGGGC 
AGAAGGAGAC CCCTGCCAAG GATTCACGCT CATGACCAGC AACTCCGGGC ACAAGCAGAG 
CGACAGGCAG TGAACTTCGT GGTGCAAGGC TCCGCTGCTG ACCTCTGCAA GCTGGCCATG 
ATCCATGTCT TCACTGCAGT GGCTGCTTCC CACACCTTGA CGGCCAGGCT GGTGGCCCAG 
ATCCATGATG AGCTGCTGTT TGAAGTGGAA GATCCGCAGA TCCCGGAGTG TGCAGCTCTC 
GTCAGGAGGA CCATGGAGTC CTTGGAACAG GTGCAGGCAT TGGAGCTGCA GCTTCAGGTA 
CCCCTCAAGG TGAGCCTGAG TGCCGGCCGC TCATGGGGAC ACCTGGTGCC ACTGCAGGAG 
GCCTGGGGCC CTCCGCCAGG CCCATGTCGC ACTGAGTCTC CCAGCAACAG CCTGGCTGCC 
CCTGGGTCCC CTGCCAGCAC CCAGCCCCCA CCCCTGCATT TTTCGCCTTC ATTTTGTCTG 
TAG