PADI6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PADI6
Entrez Gene ID	353238
Full Name	peptidyl arginine deiminase 6
Synonyms	PREMBL2,hPADVI
General protein information	Preferred Names peptidyl arginine deiminase 6 Names protein-arginine deiminase type-6 peptidyl arginine deiminase, type VI peptidyl arginine deiminase-like protein peptidylarginine deiminase type 6
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.13
Summary	This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. This protein may play a role in cytoskeletal reorganization in the egg and in early embryo development. [provided by RefSeq, Sep 2012].
Disorder MIM:	610363
Disorder Html:	Preimplantation embryonic lethality 2, 617234 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_207421.4	NP_997304.3	protein-arginine deiminase type-6

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PADI6	XP_524564.2
Canis lupus familiaris (dog)	PADI6	NP_001091016.1
Mus musculus (house mouse)	Padi6	NP_694746.2
Rattus norvegicus (Norway rat)	Padi6	NP_001178695.1
	PADI6	XP_001089244.1
Homo sapiens (human)	PADI6	NP_997304.3
Bos taurus (cattle)	PADI6	XP_002685843.1

Gene Ontology
Bibliography

Related articles in PubMed

The human knockout phenotype of PADI6 is female sterility caused by cleavage failure of their fertilized eggs.
Maddirevula S, Coskun S, Awartani K, Alsaif H, Abdulwahab FM, Alkuraya FS
Clinical genetics91(2)344-345(2017 Feb)

Mutations in PADI6 Cause Female Infertility Characterized by Early Embryonic Arrest.
Xu Y, Shi Y, Fu J, Yu M, Feng R, Sang Q, Liang B, Chen B, Qu R, Li B, Yan Z, Mao X, Kuang Y, Jin L, He L, Sun X, Wang L
American journal of human genetics99(3)744-752(2016 Sep)

Identification and structural characterization of two 14-3-3 binding sites in the human peptidylarginine deiminase type VI.
Rose R, Rose M, Ottmann C
Journal of structural biology180(1)65-72(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in PADI6 Cause Female Infertility Characterized by Early Embryonic Arrest
Title: Mutations in PADI6 Cause Female Infertility Characterized by Early Embryonic Arrest.

Crystallographic determination of 14-3-3-sigma binding sites in the human peptidylarginine deiminase type VI.
Title: Identification and structural characterization of two 14-3-3 binding sites in the human peptidylarginine deiminase type VI.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Common variants on 1p36 and 1q42 are associated with cutaneous basal cell carcinoma but not with melanoma or pigmentation traits.

This study is the first description of the human PADI6 gene and encoded protein, and the first step towards a better understanding of the coordinated regulation of PADI gene expression.
Title: Comparative analysis of the mouse and human peptidylarginine deiminase gene clusters reveals highly conserved non-coding segments and a new human gene, PADI6.

Cloning, gene organization and expression of peptidylarginine deiminase type 6.
Title: cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI.

The following PADI6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PADI6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu28454	NM_207421.4 Latest version!	Homo sapiens peptidyl arginine deiminase 6 (PADI6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PADI6 ( NM_207421.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_997304.3 Homo sapiens peptidyl arginine deiminase 6 (PADI6), mRNA.

Price & Availability↑

CloneID	OHu28454
Clone ID Related Accession (Same CDS sequence)	NM_207421.4
Accession Version	NM_207421.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	protein-arginine deiminase type-6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY443100.1 and AY422079.1. This sequence is a reference standard in the RefSeqGene project. On Feb 20, 2014 this sequence version replaced NM_207421.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY443100.1, AY422079.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000619609.1/ ENSP00000483125.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTCAGCG TGGAGGGCCG AGCCATGTCC TTCCAGAGTA TCATCCACCT GTCCCTGGAC 
AGCCCTGTCC ATGCCGTTTG TGTGTTGGGC ACAGAAATCT GCTTGGATCT CAGCGGGTGT 
GCCCCCCAGA AGTGCCAGTG CTTCACCATC CATGGCTCTG GGAGGGTCTT GATCGATGTG 
GCCAACACGG TGATTTCTGA GAAGGAGGAC GCCACCATCT GGTGGCCCCT GTCTGATCCC 
ACGTACGCCA CAGTGAAGAT GACATCGCCC AGCCCTTCCG TGGATGCGGA TAAGGTCTCG 
GTCACATACT ATGGGCCCAA CGAGGATGCC CCCGTGGGCA CAGCTGTGCT GTACCTCACT 
GGCATTGAGG TCTCTCTAGA GGTAGACATC TACCGCAATG GGCAAGTTGA GATGTCAAGT 
GACAAACAGG CTAAGAAAAA ATGGATCTGG GGTCCCAGCG GTTGGGGTGC CATCCTGCTT 
GTGAATTGCA ACCCTGCTGA TGTGGGCCAG CAACTTGAGG ACAAGAAAAC CAAGAAAGTG 
ATCTTTTCAG AGGAAATAAC GAATCTGTCC CAGATGACTC TGAATGTCCA AGGCCCCAGC 
TGTATCTTAA AGAAATATCG GCTAGTCCTC CATACCTCCA AGGAAGAGTC GAAGAAGGCG 
AGAGTCTACT GGCCCCAAAA AGACAACTCC AGTACCTTTG AGTTGGTGCT GGGGCCCGAC 
CAGCACGCCT ATACCTTGGC CCTCCTCGGG AACCACTTGA AGGAGACTTT CTACGTTGAA 
GCTATAGCAT TCCCATCTGC CGAATTCTCA GGCCTCATCT CCTACTCTGT GTCCCTGGTG 
GAGGAGTCTC AAGACCCGTC AATTCCAGAG ACTGTGCTGT ACAAAGACAC GGTGGTGTTC 
CGGGTGGCTC CCTGTGTCTT CATTCCCTGT ACCCAGGTGC CTCTGGAGGT TTACCTGTGC 
AGGGAGCTGC AGCTGCAGGG TTTTGTGGAC ACAGTGACGA AGCTGAGTGA GAAGAGCAAC 
AGCCAGGTGG CATCTGTCTA TGAGGACCCC AACCGCCTGG GCAGGTGGCT CCAGGATGAG 
ATGGCCTTCT GCTACACCCA GGCTCCCCAC AAGACAACGT CCTTGATCCT CGACACACCT 
CAGGCCGCCG ATCTCGATGA GTTCCCCATG AAGTACTCAC TGAGCCCTGG TATTGGCTAC 
ATGATCCAGG ACACTGAGGA CCATAAAGTG GCCAGCATGG ATTCCATTGG GAACCTGATG 
GTGTCCCCAC CTGTCAAGGT CCAAGGGAAA GAGTACCCGC TGGGCAGAGT CCTCATTGGC 
AGCAGCTTTT ACCCCAGCGC AGAGGGCCGG GCCATGAGTA AGACCCTCCG AGACTTCCTC 
TATGCCCAGC AGGTCCAAGC GCCGGTGGAG CTCTACTCAG ATTGGCTAAT GACTGGCCAC 
GTGGATGAGT TCATGTGCTT CATCCCCACA GATGACAAGA ATGAGGGCAA AAAGGGCTTC 
CTGCTGCTCC TGGCCAGCCC CAGTGCCTGC TATAAACTGT TCCGAGAGAA ACAGAAGGAA 
GGCTATGGCG ACGCTCTTCT GTTTGATGAG CTTAGAGCAG ATCAGCTCCT GTCTAATGGA 
AGGGAAGCCA AAACCATCGA CCAACTTCTG GCTGATGAAA GCCTGAAGAA GCAGAATGAA 
TACGTGGAGA AGTGCATTCA CCTGAACCGT GACATCCTGA AGACGGAGCT GGGCCTGGTG 
GAACAGGACA TCATCGAGAT TCCCCAGCTG TTCTGCTTGG AGAAGCTGAC TAACATCCCC 
TCTGACCAGC AGCCCAAGAG GTCCTTTGCG AGGCCATACT TCCCTGACCT GTTGCGGATG 
ATTGTGATGG GCAAGAACCT GGGGATCCCC AAGCCTTTTG GGCCCCAAAT CAAGGGGACC 
TGCTGCCTGG AAGAAAAGAT TTGCTGCTTG CTGGAGCCCC TGGGCTTCAA GTGCACCTTC 
ATCAATGACT TTGACTGTTA CCTGACAGAG GTCGGAGACA TCTGTGCCTG TGCCAACATC 
CGCCGGGTGC CCTTTGCCTT CAAATGGTGG AAGATGGTAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997304.3
CDS	51..2135
Translation	MVSVEGRAMSFQSIIHLSLDSPVHAVCVLGTEICLDLSGCAPQKCQCFTIHGSGRVLIDVANTVISEKEDATIWWPLSDPTYATVKMTSPSPSVDADKVSVTYYGPNEDAPVGTAVLYLTGIEVSLEVDIYRNGQVEMSSDKQAKKKWIWGPSGWGAILLVNCNPADVGQQLEDKKTKKVIFSEEITNLSQMTLNVQGPSCILKKYRLVLHTSKEESKKARVYWPQKDNSSTFELVLGPDQHAYTLALLGNHLKETFYVEAIAFPSAEFSGLISYSVSLVEESQDPSIPETVLYKDTVVFRVAPCVFIPCTQVPLEVYLCRELQLQGFVDTVTKLSEKSNSQVASVYEDPNRLGRWLQDEMAFCYTQAPHKTTSLILDTPQAADLDEFPMKYSLSPGIGYMIQDTEDHKVASMDSIGNLMVSPPVKVQGKEYPLGRVLIGSSFYPSAEGRAMSKTLRDFLYAQQVQAPVELYSDWLMTGHVDEFMCFIPTDDKNEGKKGFLLLLASPSACYKLFREKQKEGYGDALLFDELRADQLLSNGREAKTIDQLLADESLKKQNEYVEKCIHLNRDILKTELGLVEQDIIEIPQLFCLEKLTNIPSDQQPKRSFARPYFPDLLRMIVMGKNLGIPKPFGPQIKGTCCLEEKICCLLEPLGFKCTFINDFDCYLTEVGDICACANIRRVPFAFKWWKMVP

Target ORF information:

RefSeq Version	NM_207421.4
Organism	Homo sapiens(human)
Definition	Homo sapiens peptidyl arginine deiminase 6 (PADI6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207421.4

ORF Insert Sequence:

ATGGTCAGCG TGGAGGGCCG AGCCATGTCC TTCCAGAGTA TCATCCACCT GTCCCTGGAC 
AGCCCTGTCC ATGCCGTTTG TGTGTTGGGC ACAGAAATCT GCTTGGATCT CAGCGGGTGT 
GCCCCCCAGA AGTGCCAGTG CTTCACCATC CATGGCTCTG GGAGGGTCTT GATCGATGTG 
GCCAACACGG TGATTTCTGA GAAGGAGGAC GCCACCATCT GGTGGCCCCT GTCTGATCCC 
ACGTACGCCA CAGTGAAGAT GACATCGCCC AGCCCTTCCG TGGATGCGGA TAAGGTCTCG 
GTCACATACT ATGGGCCCAA CGAGGATGCC CCCGTGGGCA CAGCTGTGCT GTACCTCACT 
GGCATTGAGG TCTCTCTAGA GGTAGACATC TACCGCAATG GGCAAGTTGA GATGTCAAGT 
GACAAACAGG CTAAGAAAAA ATGGATCTGG GGTCCCAGCG GTTGGGGTGC CATCCTGCTT 
GTGAATTGCA ACCCTGCTGA TGTGGGCCAG CAACTTGAGG ACAAGAAAAC CAAGAAAGTG 
ATCTTTTCAG AGGAAATAAC GAATCTGTCC CAGATGACTC TGAATGTCCA AGGCCCCAGC 
TGTATCTTAA AGAAATATCG GCTAGTCCTC CATACCTCCA AGGAAGAGTC GAAGAAGGCG 
AGAGTCTACT GGCCCCAAAA AGACAACTCC AGTACCTTTG AGTTGGTGCT GGGGCCCGAC 
CAGCACGCCT ATACCTTGGC CCTCCTCGGG AACCACTTGA AGGAGACTTT CTACGTTGAA 
GCTATAGCAT TCCCATCTGC CGAATTCTCA GGCCTCATCT CCTACTCTGT GTCCCTGGTG 
GAGGAGTCTC AAGACCCGTC AATTCCAGAG ACTGTGCTGT ACAAAGACAC GGTGGTGTTC 
CGGGTGGCTC CCTGTGTCTT CATTCCCTGT ACCCAGGTGC CTCTGGAGGT TTACCTGTGC 
AGGGAGCTGC AGCTGCAGGG TTTTGTGGAC ACAGTGACGA AGCTGAGTGA GAAGAGCAAC 
AGCCAGGTGG CATCTGTCTA TGAGGACCCC AACCGCCTGG GCAGGTGGCT CCAGGATGAG 
ATGGCCTTCT GCTACACCCA GGCTCCCCAC AAGACAACGT CCTTGATCCT CGACACACCT 
CAGGCCGCCG ATCTCGATGA GTTCCCCATG AAGTACTCAC TGAGCCCTGG TATTGGCTAC 
ATGATCCAGG ACACTGAGGA CCATAAAGTG GCCAGCATGG ATTCCATTGG GAACCTGATG 
GTGTCCCCAC CTGTCAAGGT CCAAGGGAAA GAGTACCCGC TGGGCAGAGT CCTCATTGGC 
AGCAGCTTTT ACCCCAGCGC AGAGGGCCGG GCCATGAGTA AGACCCTCCG AGACTTCCTC 
TATGCCCAGC AGGTCCAAGC GCCGGTGGAG CTCTACTCAG ATTGGCTAAT GACTGGCCAC 
GTGGATGAGT TCATGTGCTT CATCCCCACA GATGACAAGA ATGAGGGCAA AAAGGGCTTC 
CTGCTGCTCC TGGCCAGCCC CAGTGCCTGC TATAAACTGT TCCGAGAGAA ACAGAAGGAA 
GGCTATGGCG ACGCTCTTCT GTTTGATGAG CTTAGAGCAG ATCAGCTCCT GTCTAATGGA 
AGGGAAGCCA AAACCATCGA CCAACTTCTG GCTGATGAAA GCCTGAAGAA GCAGAATGAA 
TACGTGGAGA AGTGCATTCA CCTGAACCGT GACATCCTGA AGACGGAGCT GGGCCTGGTG 
GAACAGGACA TCATCGAGAT TCCCCAGCTG TTCTGCTTGG AGAAGCTGAC TAACATCCCC 
TCTGACCAGC AGCCCAAGAG GTCCTTTGCG AGGCCATACT TCCCTGACCT GTTGCGGATG 
ATTGTGATGG GCAAGAACCT GGGGATCCCC AAGCCTTTTG GGCCCCAAAT CAAGGGGACC 
TGCTGCCTGG AAGAAAAGAT TTGCTGCTTG CTGGAGCCCC TGGGCTTCAA GTGCACCTTC 
ATCAATGACT TTGACTGTTA CCTGACAGAG GTCGGAGACA TCTGTGCCTG TGCCAACATC 
CGCCGGGTGC CCTTTGCCTT CAAATGGTGG AAGATGGTAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The human knockout phenotype of PADI6 is female sterility caused by cleavage failure of their fertilized eggs.
Clinical genetics91(2)344-345(2017 Feb)
Maddirevula S,Coskun S,Awartani K,Alsaif H,Abdulwahab FM,Alkuraya FS

Mutations in PADI6 Cause Female Infertility Characterized by Early Embryonic Arrest.
American journal of human genetics99(3)744-752(2016 Sep)
Xu Y,Shi Y,Fu J,Yu M,Feng R,Sang Q,Liang B,Chen B,Qu R,Li B,Yan Z,Mao X,Kuang Y,Jin L,He L,Sun X,Wang L

Identification and structural characterization of two 14-3-3 binding sites in the human peptidylarginine deiminase type VI.
Journal of structural biology180(1)65-72(2012 Oct)
Rose R,Rose M,Ottmann C