HACL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HACL1
Entrez Gene ID	26061
Full Name	2-hydroxyacyl-CoA lyase 1
Synonyms	2-HPCL,HPCL,HPCL2,PHYH2
General protein information	Preferred Names 2-hydroxyacyl-CoA lyase 1 Names 2-hydroxyacyl-CoA lyase 1 1600020H07Rik 2-hydroxyphytanol-CoA lyase 2-hydroxyphytanoyl-CoA lyase phytanoyl-CoA 2-hydroxylase 2 phytanoyl-CoA hydroxylase 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p25.1
Disorder MIM:	604300

mRNA and Protein(s)

mRNA	Protein	Name
NM_001284415.1	NP_001271344.1	2-hydroxyacyl-CoA lyase 1 isoform c
NM_001284413.1	NP_001271342.1	2-hydroxyacyl-CoA lyase 1 isoform b
NM_001284413.2	NP_001271342.1	2-hydroxyacyl-CoA lyase 1 isoform b
NM_001284416.1	NP_001271345.1	2-hydroxyacyl-CoA lyase 1 isoform d
NM_012260.3	NP_036392.2	2-hydroxyacyl-CoA lyase 1 isoform a
NM_001284415.2	NP_001271344.1	2-hydroxyacyl-CoA lyase 1 isoform c
NM_001284416.2	NP_001271345.1	2-hydroxyacyl-CoA lyase 1 isoform d
NM_012260.4	NP_036392.2	2-hydroxyacyl-CoA lyase 1 isoform a

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HACL1	XP_001160528.1
Drosophila melanogaster (fruit fly)	CG11208	NP_611460.1
Schizosaccharomyces pombe (fission yeast)	SPBC725.04	NP_595484.1
Homo sapiens (human)	HACL1	NP_036392.2
Bos taurus (cattle)	HACL1	NP_001092419.1
Danio rerio (zebrafish)	hacl1	NP_998250.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010368	XP_311576.3
Eremothecium gossypii	AGOS_AEL055C	NP_984806.1
Gallus gallus (chicken)	HACL1	NP_001186557.1
Arabidopsis thaliana (thale cress)	AT5G17380	NP_197240.1
Saccharomyces cerevisiae (baker's yeast)	YEL020C	NP_010895.1
Xenopus tropicalis (tropical clawed frog)	hacl1	NP_001017332.1
Canis lupus familiaris (dog)	HACL1	XP_534256.4
Mus musculus (house mouse)	Hacl1	NP_064359.2
Rattus norvegicus (Norway rat)	Hacl1	NP_445945.1
Caenorhabditis elegans (roundworm)	CELE_B0334.3	NP_496454.1

Gene Ontology
Bibliography

Related articles in PubMed

An inter-species protein-protein interaction network across vast evolutionary distance.
Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Ta?an M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barab?si AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M
Molecular systems biology12(4)865(2016 Apr)

RP-HPLC-fluorescence analysis of aliphatic aldehydes: application to aldehyde-generating enzymes HACL1 and SGPL1.
Mezzar S, de Schryver E, Van Veldhoven PP
Journal of lipid research55(3)573-82(2014 Mar)

Role of thiamine pyrophosphate in oligomerisation, functioning and import of peroxisomal 2-hydroxyacyl-CoA lyase.
Fraccascia P, Casteels M, De Schryver E, Van Veldhoven PP
Biochimica et biophysica acta1814(10)1226-33(2011 Oct)

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Ghosh D, Berg JM
Journal of the American Chemical Society132(11)3973-9(2010 Mar)

Biochemistry of mammalian peroxisomes revisited.
Wanders RJ, Waterham HR
Annual review of biochemistry75295-332(2006)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that a easy assay for 2-hydroxyacyl-CoA lyase (HACL1) and sphingosine-1-phosphate lyase (SGPL1) activities was developed.
Title: RP-HPLC-fluorescence analysis of aliphatic aldehydes: application to aldehyde-generating enzymes HACL1 and SGPL1.

Transport of thiamine pyrophosphate into peroxisomes is dependent on HACL1 import, without requirement of a specific solute transporter.
Title: Role of thiamine pyrophosphate in oligomerisation, functioning and import of peroxisomal 2-hydroxyacyl-CoA lyase.

2-hydroxyphytanoyl-CoA lyase has a role in the breakdown of long chain 2-hydroxyfatty acids
Title: Breakdown of 2-hydroxylated straight chain fatty acids via peroxisomal 2-hydroxyphytanoyl-CoA lyase: a revised pathway for the alpha-oxidation of straight chain fatty acids.

peroxisomal thiamine-pyrophosphate depending enzyme required for the degradation of phytanic acid, catalyzing the cleavage of 2-hydroxyphytanoyl-CoA lyase into formyl-CoA and pristanal
Title: Purification, molecular cloning, and expression of 2-hydroxyphytanoyl-CoA lyase, a peroxisomal thiamine pyrophosphate-dependent enzyme that catalyzes the carbon-carbon bond cleavage during alpha-oxidation of 3-methyl-branched fatty acids.

The following HACL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HACL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01074	NM_001284415.2 Latest version!	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu01094	NM_001284416.2 Latest version!	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu05364	NM_001284413.2 Latest version!	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu06141	NM_012260.4 Latest version!	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu05364	NM_001284413.1	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu01074	NM_001284415.1	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu01094	NM_001284416.1	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu06141	NM_012260.3	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HACL1 ( NM_001284415.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271344.1 Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01074
Clone ID Related Accession (Same CDS sequence)	NM_001284415.1 , NM_001284415.2
Accession Version	NM_001284415.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-15
Organism	Homo sapiens(human)
Product	2-hydroxyacyl-CoA lyase 1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB241165.1, DA609383.1, AK302086.1, BC001627.1, AJ131753.1 and AA643224.1. On Oct 1, 2013 this sequence version replaced XM_005265040.1. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the central coding region, compared to variant 1, resulting in an isoform (c) that is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: AK302086.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC AGGAGTCTGC 
CTTGTTGTTT CTGGCCCAGG TCTCATCCAT GCCTTGGGCG GTATGGCAAA TGCAAACATG 
AACTGCTGGC CCTTGCTTGT GATTGGTGGT TCCTCTGAAA GAAACCAAGA AACAATGGGA 
GCTTTCCAGG AGTTTCCTCA GGCAGTGAGA AGCAGTATCT ATGGTCGTCC AGGTGCTTGC 
TATGTTGACA TACCAGCAGA TTTTGTGAAC CTTCAGGTGA ATGTGAATTC TATAAAGTAC 
ATGGAACGCT GCATGTCACC TCCTATTAGC ATGGCAGAAA CCTCTGCTGT GTGCACGGCG 
GCTTCTGTTA TTAGGAATGC CAAACAACCC CTTCTTATCA TCGGGAAAGG TGCTGCTTAC 
GCTCATGCAG AAGAGAGTAT CAAGAAATTG GTGGAGCAAT ATAAACTGCC ATTTTTGCCC 
ACCCCTATGG GGAAGGGTGT TGTCCCTGAC AACCATCCAT ACTGTGTAGG TGCAGCCAGA 
TCCAGGGCTT TGCAATTTGC TGATGTAATT GTGTTATTTG GTGCCAGACT AAATTGGATT 
TTACATTTTG GACTGCCTCC AAGATATCAG CCAGATGTGA AGTTTATCCA GGTTGATATC 
TGTGCAGAAG AATTGGGGAA TAATGTAAAG CCCGCTGTTA CTTTGCTAGG AAACATACAT 
GCTGTCACTA AGCAGGAACT AGCTTCTAAA AAATCCCTGC CTATGAATTA TTACACAGTA 
TTCTACCATG TTCAAGAACA ACTACCTAGA GACTGTTTCG TGGTAAGTGA AGGAGCAAAT 
ACTATGGACA TTGGACGGAC TGTGCTTCAG AACTACCTTC CTCGTCACAG GCTTGATGCT 
GGTACTTTCG GAACAATGGG AGTTGGTTTG GGATTTGCTA TTGCAGCTGC CGTGGTGGCT 
AAAGATAGAA GCCCTGGGCA ATGGATCATC TGTGTGGAAG GAGACAGTGC ATTTGGGTTT 
TCTGGCATGG AGGTAGAAAC CATCTGCAGG TACAACTTGC CAATCATACT GTTGGTAGTG 
AATAACAATG GAATTTACCA AGGTTTTGAT ACAGATACTT GGAAAGAAAT GTTAAAATTT 
CAAGATGCTA CTGCAGTGGT CCCTCCAATG TGTTTGCTGC CAAATTCACA TTATGAGCAA 
GTCATGACTG CATTTGGAGG CAAAGGGTAT TTTGTACAAA CACCAGAAGA ACTCCAAAAA 
TCCCTGAGGC AGAGCCTAGC AGACACAACT AAACCTTCTC TTATCAACAT CATGATTGAG 
CCACAAGCCA CACGGAAGGC CCAGGATTTT CATTGGCTGA CCCGCTCTAA TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271344.1
CDS	390..1946
Translation	MPDSNFAERSEEQVSGAKVIAQALKTQDVEYIFGIVGIPVTEIAIAAQQLGIKYIGMRNEQAACYAASAIGYLTSRPGVCLVVSGPGLIHALGGMANANMNCWPLLVIGGSSERNQETMGAFQEFPQAVRSSIYGRPGACYVDIPADFVNLQVNVNSIKYMERCMSPPISMAETSAVCTAASVIRNAKQPLLIIGKGAAYAHAEESIKKLVEQYKLPFLPTPMGKGVVPDNHPYCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQPDVKFIQVDICAEELGNNVKPAVTLLGNIHAVTKQELASKKSLPMNYYTVFYHVQEQLPRDCFVVSEGANTMDIGRTVLQNYLPRHRLDAGTFGTMGVGLGFAIAAAVVAKDRSPGQWIICVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKFQDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQKSLRQSLADTTKPSLINIMIEPQATRKAQDFHWLTRSNM

Target ORF information:

RefSeq Version	NM_001284415.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284415.1

ORF Insert Sequence:

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC AGGAGTCTGC 
CTTGTTGTTT CTGGCCCAGG TCTCATCCAT GCCTTGGGCG GTATGGCAAA TGCAAACATG 
AACTGCTGGC CCTTGCTTGT GATTGGTGGT TCCTCTGAAA GAAACCAAGA AACAATGGGA 
GCTTTCCAGG AGTTTCCTCA GGCAGTGAGA AGCAGTATCT ATGGTCGTCC AGGTGCTTGC 
TATGTTGACA TACCAGCAGA TTTTGTGAAC CTTCAGGTGA ATGTGAATTC TATAAAGTAC 
ATGGAACGCT GCATGTCACC TCCTATTAGC ATGGCAGAAA CCTCTGCTGT GTGCACGGCG 
GCTTCTGTTA TTAGGAATGC CAAACAACCC CTTCTTATCA TCGGGAAAGG TGCTGCTTAC 
GCTCATGCAG AAGAGAGTAT CAAGAAATTG GTGGAGCAAT ATAAACTGCC ATTTTTGCCC 
ACCCCTATGG GGAAGGGTGT TGTCCCTGAC AACCATCCAT ACTGTGTAGG TGCAGCCAGA 
TCCAGGGCTT TGCAATTTGC TGATGTAATT GTGTTATTTG GTGCCAGACT AAATTGGATT 
TTACATTTTG GACTGCCTCC AAGATATCAG CCAGATGTGA AGTTTATCCA GGTTGATATC 
TGTGCAGAAG AATTGGGGAA TAATGTAAAG CCCGCTGTTA CTTTGCTAGG AAACATACAT 
GCTGTCACTA AGCAGGAACT AGCTTCTAAA AAATCCCTGC CTATGAATTA TTACACAGTA 
TTCTACCATG TTCAAGAACA ACTACCTAGA GACTGTTTCG TGGTAAGTGA AGGAGCAAAT 
ACTATGGACA TTGGACGGAC TGTGCTTCAG AACTACCTTC CTCGTCACAG GCTTGATGCT 
GGTACTTTCG GAACAATGGG AGTTGGTTTG GGATTTGCTA TTGCAGCTGC CGTGGTGGCT 
AAAGATAGAA GCCCTGGGCA ATGGATCATC TGTGTGGAAG GAGACAGTGC ATTTGGGTTT 
TCTGGCATGG AGGTAGAAAC CATCTGCAGG TACAACTTGC CAATCATACT GTTGGTAGTG 
AATAACAATG GAATTTACCA AGGTTTTGAT ACAGATACTT GGAAAGAAAT GTTAAAATTT 
CAAGATGCTA CTGCAGTGGT CCCTCCAATG TGTTTGCTGC CAAATTCACA TTATGAGCAA 
GTCATGACTG CATTTGGAGG CAAAGGGTAT TTTGTACAAA CACCAGAAGA ACTCCAAAAA 
TCCCTGAGGC AGAGCCTAGC AGACACAACT AAACCTTCTC TTATCAACAT CATGATTGAG 
CCACAAGCCA CACGGAAGGC CCAGGATTTT CATTGGCTGA CCCGCTCTAA TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACL1 ( NM_001284415.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271344.1 Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01074
Clone ID Related Accession (Same CDS sequence)	NM_001284415.1 , NM_001284415.2
Accession Version	NM_001284415.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	2-hydroxyacyl-CoA lyase 1 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA609383.1, AK302086.1, BC001627.1, AJ131753.1, AA643224.1 and AC027129.6. On May 31, 2019 this sequence version replaced NM_001284415.1. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the central coding region, compared to variant 1, resulting in an isoform (c) that is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: AK302086.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC AGGAGTCTGC 
CTTGTTGTTT CTGGCCCAGG TCTCATCCAT GCCTTGGGCG GTATGGCAAA TGCAAACATG 
AACTGCTGGC CCTTGCTTGT GATTGGTGGT TCCTCTGAAA GAAACCAAGA AACAATGGGA 
GCTTTCCAGG AGTTTCCTCA GGCAGTGAGA AGCAGTATCT ATGGTCGTCC AGGTGCTTGC 
TATGTTGACA TACCAGCAGA TTTTGTGAAC CTTCAGGTGA ATGTGAATTC TATAAAGTAC 
ATGGAACGCT GCATGTCACC TCCTATTAGC ATGGCAGAAA CCTCTGCTGT GTGCACGGCG 
GCTTCTGTTA TTAGGAATGC CAAACAACCC CTTCTTATCA TCGGGAAAGG TGCTGCTTAC 
GCTCATGCAG AAGAGAGTAT CAAGAAATTG GTGGAGCAAT ATAAACTGCC ATTTTTGCCC 
ACCCCTATGG GGAAGGGTGT TGTCCCTGAC AACCATCCAT ACTGTGTAGG TGCAGCCAGA 
TCCAGGGCTT TGCAATTTGC TGATGTAATT GTGTTATTTG GTGCCAGACT AAATTGGATT 
TTACATTTTG GACTGCCTCC AAGATATCAG CCAGATGTGA AGTTTATCCA GGTTGATATC 
TGTGCAGAAG AATTGGGGAA TAATGTAAAG CCCGCTGTTA CTTTGCTAGG AAACATACAT 
GCTGTCACTA AGCAGGAACT AGCTTCTAAA AAATCCCTGC CTATGAATTA TTACACAGTA 
TTCTACCATG TTCAAGAACA ACTACCTAGA GACTGTTTCG TGGTAAGTGA AGGAGCAAAT 
ACTATGGACA TTGGACGGAC TGTGCTTCAG AACTACCTTC CTCGTCACAG GCTTGATGCT 
GGTACTTTCG GAACAATGGG AGTTGGTTTG GGATTTGCTA TTGCAGCTGC CGTGGTGGCT 
AAAGATAGAA GCCCTGGGCA ATGGATCATC TGTGTGGAAG GAGACAGTGC ATTTGGGTTT 
TCTGGCATGG AGGTAGAAAC CATCTGCAGG TACAACTTGC CAATCATACT GTTGGTAGTG 
AATAACAATG GAATTTACCA AGGTTTTGAT ACAGATACTT GGAAAGAAAT GTTAAAATTT 
CAAGATGCTA CTGCAGTGGT CCCTCCAATG TGTTTGCTGC CAAATTCACA TTATGAGCAA 
GTCATGACTG CATTTGGAGG CAAAGGGTAT TTTGTACAAA CACCAGAAGA ACTCCAAAAA 
TCCCTGAGGC AGAGCCTAGC AGACACAACT AAACCTTCTC TTATCAACAT CATGATTGAG 
CCACAAGCCA CACGGAAGGC CCAGGATTTT CATTGGCTGA CCCGCTCTAA TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271344.1
CDS	107..1663
Translation	MPDSNFAERSEEQVSGAKVIAQALKTQDVEYIFGIVGIPVTEIAIAAQQLGIKYIGMRNEQAACYAASAIGYLTSRPGVCLVVSGPGLIHALGGMANANMNCWPLLVIGGSSERNQETMGAFQEFPQAVRSSIYGRPGACYVDIPADFVNLQVNVNSIKYMERCMSPPISMAETSAVCTAASVIRNAKQPLLIIGKGAAYAHAEESIKKLVEQYKLPFLPTPMGKGVVPDNHPYCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQPDVKFIQVDICAEELGNNVKPAVTLLGNIHAVTKQELASKKSLPMNYYTVFYHVQEQLPRDCFVVSEGANTMDIGRTVLQNYLPRHRLDAGTFGTMGVGLGFAIAAAVVAKDRSPGQWIICVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKFQDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQKSLRQSLADTTKPSLINIMIEPQATRKAQDFHWLTRSNM

Target ORF information:

RefSeq Version	NM_001284415.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284415.2

ORF Insert Sequence:

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC AGGAGTCTGC 
CTTGTTGTTT CTGGCCCAGG TCTCATCCAT GCCTTGGGCG GTATGGCAAA TGCAAACATG 
AACTGCTGGC CCTTGCTTGT GATTGGTGGT TCCTCTGAAA GAAACCAAGA AACAATGGGA 
GCTTTCCAGG AGTTTCCTCA GGCAGTGAGA AGCAGTATCT ATGGTCGTCC AGGTGCTTGC 
TATGTTGACA TACCAGCAGA TTTTGTGAAC CTTCAGGTGA ATGTGAATTC TATAAAGTAC 
ATGGAACGCT GCATGTCACC TCCTATTAGC ATGGCAGAAA CCTCTGCTGT GTGCACGGCG 
GCTTCTGTTA TTAGGAATGC CAAACAACCC CTTCTTATCA TCGGGAAAGG TGCTGCTTAC 
GCTCATGCAG AAGAGAGTAT CAAGAAATTG GTGGAGCAAT ATAAACTGCC ATTTTTGCCC 
ACCCCTATGG GGAAGGGTGT TGTCCCTGAC AACCATCCAT ACTGTGTAGG TGCAGCCAGA 
TCCAGGGCTT TGCAATTTGC TGATGTAATT GTGTTATTTG GTGCCAGACT AAATTGGATT 
TTACATTTTG GACTGCCTCC AAGATATCAG CCAGATGTGA AGTTTATCCA GGTTGATATC 
TGTGCAGAAG AATTGGGGAA TAATGTAAAG CCCGCTGTTA CTTTGCTAGG AAACATACAT 
GCTGTCACTA AGCAGGAACT AGCTTCTAAA AAATCCCTGC CTATGAATTA TTACACAGTA 
TTCTACCATG TTCAAGAACA ACTACCTAGA GACTGTTTCG TGGTAAGTGA AGGAGCAAAT 
ACTATGGACA TTGGACGGAC TGTGCTTCAG AACTACCTTC CTCGTCACAG GCTTGATGCT 
GGTACTTTCG GAACAATGGG AGTTGGTTTG GGATTTGCTA TTGCAGCTGC CGTGGTGGCT 
AAAGATAGAA GCCCTGGGCA ATGGATCATC TGTGTGGAAG GAGACAGTGC ATTTGGGTTT 
TCTGGCATGG AGGTAGAAAC CATCTGCAGG TACAACTTGC CAATCATACT GTTGGTAGTG 
AATAACAATG GAATTTACCA AGGTTTTGAT ACAGATACTT GGAAAGAAAT GTTAAAATTT 
CAAGATGCTA CTGCAGTGGT CCCTCCAATG TGTTTGCTGC CAAATTCACA TTATGAGCAA 
GTCATGACTG CATTTGGAGG CAAAGGGTAT TTTGTACAAA CACCAGAAGA ACTCCAAAAA 
TCCCTGAGGC AGAGCCTAGC AGACACAACT AAACCTTCTC TTATCAACAT CATGATTGAG 
CCACAAGCCA CACGGAAGGC CCAGGATTTT CATTGGCTGA CCCGCTCTAA TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACL1 ( NM_001284416.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271345.1 Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu01094
Clone ID Related Accession (Same CDS sequence)	NM_001284416.1 , NM_001284416.2
Accession Version	NM_001284416.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	2-hydroxyacyl-CoA lyase 1 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB241165.1, DA609383.1, AK301990.1 and AA643224.1. On Oct 1, 2013 this sequence version replaced XM_005265041.1. Transcript Variant: This variant (4) lacks three alternate exons that result in the loss of an in-frame segment in the central coding region, compared to variant 1. The encoded isoform (d) is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: AK301990.1, SRR1660805.115553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2152474 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC AGGAGTCTGC 
CTTGTTGTTT CTGGCCCAGG TCTCATCCAT GCCTTGGGCG GTATGGCAAA TGCAAACATG 
AACTGCTGGT ACATGGAACG CTGCATGTCA CCTCCTATTA GCATGGCAGA AACCTCTGCT 
GTGTGCACGG CGGCTTCTGT TATTAGGAAT GCCAAACAAC CCCTTCTTAT CATCGGGAAA 
GGTGCTGCTT ACGCTCATGC AGAAGAGAGT ATCAAGAAAT TGGTGGAGCA ATATAAACTG 
CCATTTTTGC CCACCCCTAT GGGGAAGGGT GTTGTCCCTG ACAACCATCC ATACTGTGTA 
GGTGCAGCCA GATCCAGGGC TTTGCAATTT GCTGATGTAA TTGTGTTATT TGGTGCCAGA 
CTAAATTGGA TTTTACATTT TGGACTGCCT CCAAGATATC AGCCAGATGT GAAGTTTATC 
CAGGTTGATA TCTGTGCAGA AGAATTGGGG AATAATGTAA AGCCCGCTGT TACTTTGCTA 
GGAAACATAC ATGCTGTCAC TAAGCAGCTT TTAGAGGAAC TTGATAAAAC ACCATGGCAG 
TATCCTCCAG AGAGCAAGTG GTGGAAAACT CTGAGAGAAA AAATGAAGAG CAATGAAGCT 
GCATCCAAGG AACTAGCTTC TAAAAAATCC CTGCCTATGA ATTATTACAC AGTATTCTAC 
CATGTTCAAG AACAACTACC TAGAGACTGT TTCGTGGTAA GTGAAGGAGC AAATACTATG 
GACATTGGAC GGACTGTGCT TCAGAACTAC CTTCCTCGTC ACAGGCTTGA TGCTGGTACT 
TTCGGAACAA TGGGAGTTGG TTTGGGATTT GCTATTGCAG CTGCCGTGGT GGCTAAAGAT 
AGAAGCCCTG GGCAATGGAT CATCTGTGTG GAAGGAGACA GTGCATTTGG GTTTTCTGGC 
ATGGAGGTAG AAACCATCTG CAGGTACAAC TTGCCAATCA TACTGTTGGT AGTGAATAAC 
AATGGAATTT ACCAAGGTTT TGATACAGAT ACTTGGAAAG AAATGTTAAA ATTTCAAGAT 
GCTACTGCAG TGGTCCCTCC AATGTGTTTG CTGCCAAATT CACATTATGA GCAAGTCATG 
ACTGCATTTG GAGGCAAAGG GTATTTTGTA CAAACACCAG AAGAACTCCA AAAATCCCTG 
AGGCAGAGCC TAGCAGACAC AACTAAACCT TCTCTTATCA ACATCATGAT TGAGCCACAA 
GCCACACGGA AGGCCCAGGA TTTTCATTGG CTGACCCGCT CTAATATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271345.1
CDS	390..1880
Translation	MPDSNFAERSEEQVSGAKVIAQALKTQDVEYIFGIVGIPVTEIAIAAQQLGIKYIGMRNEQAACYAASAIGYLTSRPGVCLVVSGPGLIHALGGMANANMNCWYMERCMSPPISMAETSAVCTAASVIRNAKQPLLIIGKGAAYAHAEESIKKLVEQYKLPFLPTPMGKGVVPDNHPYCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQPDVKFIQVDICAEELGNNVKPAVTLLGNIHAVTKQLLEELDKTPWQYPPESKWWKTLREKMKSNEAASKELASKKSLPMNYYTVFYHVQEQLPRDCFVVSEGANTMDIGRTVLQNYLPRHRLDAGTFGTMGVGLGFAIAAAVVAKDRSPGQWIICVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKFQDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQKSLRQSLADTTKPSLINIMIEPQATRKAQDFHWLTRSNM

Target ORF information:

RefSeq Version	NM_001284416.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284416.1

ORF Insert Sequence:

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC AGGAGTCTGC 
CTTGTTGTTT CTGGCCCAGG TCTCATCCAT GCCTTGGGCG GTATGGCAAA TGCAAACATG 
AACTGCTGGT ACATGGAACG CTGCATGTCA CCTCCTATTA GCATGGCAGA AACCTCTGCT 
GTGTGCACGG CGGCTTCTGT TATTAGGAAT GCCAAACAAC CCCTTCTTAT CATCGGGAAA 
GGTGCTGCTT ACGCTCATGC AGAAGAGAGT ATCAAGAAAT TGGTGGAGCA ATATAAACTG 
CCATTTTTGC CCACCCCTAT GGGGAAGGGT GTTGTCCCTG ACAACCATCC ATACTGTGTA 
GGTGCAGCCA GATCCAGGGC TTTGCAATTT GCTGATGTAA TTGTGTTATT TGGTGCCAGA 
CTAAATTGGA TTTTACATTT TGGACTGCCT CCAAGATATC AGCCAGATGT GAAGTTTATC 
CAGGTTGATA TCTGTGCAGA AGAATTGGGG AATAATGTAA AGCCCGCTGT TACTTTGCTA 
GGAAACATAC ATGCTGTCAC TAAGCAGCTT TTAGAGGAAC TTGATAAAAC ACCATGGCAG 
TATCCTCCAG AGAGCAAGTG GTGGAAAACT CTGAGAGAAA AAATGAAGAG CAATGAAGCT 
GCATCCAAGG AACTAGCTTC TAAAAAATCC CTGCCTATGA ATTATTACAC AGTATTCTAC 
CATGTTCAAG AACAACTACC TAGAGACTGT TTCGTGGTAA GTGAAGGAGC AAATACTATG 
GACATTGGAC GGACTGTGCT TCAGAACTAC CTTCCTCGTC ACAGGCTTGA TGCTGGTACT 
TTCGGAACAA TGGGAGTTGG TTTGGGATTT GCTATTGCAG CTGCCGTGGT GGCTAAAGAT 
AGAAGCCCTG GGCAATGGAT CATCTGTGTG GAAGGAGACA GTGCATTTGG GTTTTCTGGC 
ATGGAGGTAG AAACCATCTG CAGGTACAAC TTGCCAATCA TACTGTTGGT AGTGAATAAC 
AATGGAATTT ACCAAGGTTT TGATACAGAT ACTTGGAAAG AAATGTTAAA ATTTCAAGAT 
GCTACTGCAG TGGTCCCTCC AATGTGTTTG CTGCCAAATT CACATTATGA GCAAGTCATG 
ACTGCATTTG GAGGCAAAGG GTATTTTGTA CAAACACCAG AAGAACTCCA AAAATCCCTG 
AGGCAGAGCC TAGCAGACAC AACTAAACCT TCTCTTATCA ACATCATGAT TGAGCCACAA 
GCCACACGGA AGGCCCAGGA TTTTCATTGG CTGACCCGCT CTAATATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACL1 ( NM_001284416.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271345.1 Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 4, mRNA.

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CloneID	OHu01094
Clone ID Related Accession (Same CDS sequence)	NM_001284416.1 , NM_001284416.2
Accession Version	NM_001284416.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-31
Organism	Homo sapiens(human)
Product	2-hydroxyacyl-CoA lyase 1 isoform d
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA609383.1, AK301990.1, AA643224.1 and AC027129.6. On Jun 1, 2019 this sequence version replaced NM_001284416.1. Transcript Variant: This variant (4) lacks three alternate exons that result in the loss of an in-frame segment in the central coding region, compared to variant 1. The encoded isoform (d) is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.65267.1, AK301990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2152474 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC AGGAGTCTGC 
CTTGTTGTTT CTGGCCCAGG TCTCATCCAT GCCTTGGGCG GTATGGCAAA TGCAAACATG 
AACTGCTGGT ACATGGAACG CTGCATGTCA CCTCCTATTA GCATGGCAGA AACCTCTGCT 
GTGTGCACGG CGGCTTCTGT TATTAGGAAT GCCAAACAAC CCCTTCTTAT CATCGGGAAA 
GGTGCTGCTT ACGCTCATGC AGAAGAGAGT ATCAAGAAAT TGGTGGAGCA ATATAAACTG 
CCATTTTTGC CCACCCCTAT GGGGAAGGGT GTTGTCCCTG ACAACCATCC ATACTGTGTA 
GGTGCAGCCA GATCCAGGGC TTTGCAATTT GCTGATGTAA TTGTGTTATT TGGTGCCAGA 
CTAAATTGGA TTTTACATTT TGGACTGCCT CCAAGATATC AGCCAGATGT GAAGTTTATC 
CAGGTTGATA TCTGTGCAGA AGAATTGGGG AATAATGTAA AGCCCGCTGT TACTTTGCTA 
GGAAACATAC ATGCTGTCAC TAAGCAGCTT TTAGAGGAAC TTGATAAAAC ACCATGGCAG 
TATCCTCCAG AGAGCAAGTG GTGGAAAACT CTGAGAGAAA AAATGAAGAG CAATGAAGCT 
GCATCCAAGG AACTAGCTTC TAAAAAATCC CTGCCTATGA ATTATTACAC AGTATTCTAC 
CATGTTCAAG AACAACTACC TAGAGACTGT TTCGTGGTAA GTGAAGGAGC AAATACTATG 
GACATTGGAC GGACTGTGCT TCAGAACTAC CTTCCTCGTC ACAGGCTTGA TGCTGGTACT 
TTCGGAACAA TGGGAGTTGG TTTGGGATTT GCTATTGCAG CTGCCGTGGT GGCTAAAGAT 
AGAAGCCCTG GGCAATGGAT CATCTGTGTG GAAGGAGACA GTGCATTTGG GTTTTCTGGC 
ATGGAGGTAG AAACCATCTG CAGGTACAAC TTGCCAATCA TACTGTTGGT AGTGAATAAC 
AATGGAATTT ACCAAGGTTT TGATACAGAT ACTTGGAAAG AAATGTTAAA ATTTCAAGAT 
GCTACTGCAG TGGTCCCTCC AATGTGTTTG CTGCCAAATT CACATTATGA GCAAGTCATG 
ACTGCATTTG GAGGCAAAGG GTATTTTGTA CAAACACCAG AAGAACTCCA AAAATCCCTG 
AGGCAGAGCC TAGCAGACAC AACTAAACCT TCTCTTATCA ACATCATGAT TGAGCCACAA 
GCCACACGGA AGGCCCAGGA TTTTCATTGG CTGACCCGCT CTAATATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271345.1
CDS	107..1597
Translation	MPDSNFAERSEEQVSGAKVIAQALKTQDVEYIFGIVGIPVTEIAIAAQQLGIKYIGMRNEQAACYAASAIGYLTSRPGVCLVVSGPGLIHALGGMANANMNCWYMERCMSPPISMAETSAVCTAASVIRNAKQPLLIIGKGAAYAHAEESIKKLVEQYKLPFLPTPMGKGVVPDNHPYCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQPDVKFIQVDICAEELGNNVKPAVTLLGNIHAVTKQLLEELDKTPWQYPPESKWWKTLREKMKSNEAASKELASKKSLPMNYYTVFYHVQEQLPRDCFVVSEGANTMDIGRTVLQNYLPRHRLDAGTFGTMGVGLGFAIAAAVVAKDRSPGQWIICVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKFQDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQKSLRQSLADTTKPSLINIMIEPQATRKAQDFHWLTRSNM

Target ORF information:

RefSeq Version	NM_001284416.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284416.2

ORF Insert Sequence:

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC AGGAGTCTGC 
CTTGTTGTTT CTGGCCCAGG TCTCATCCAT GCCTTGGGCG GTATGGCAAA TGCAAACATG 
AACTGCTGGT ACATGGAACG CTGCATGTCA CCTCCTATTA GCATGGCAGA AACCTCTGCT 
GTGTGCACGG CGGCTTCTGT TATTAGGAAT GCCAAACAAC CCCTTCTTAT CATCGGGAAA 
GGTGCTGCTT ACGCTCATGC AGAAGAGAGT ATCAAGAAAT TGGTGGAGCA ATATAAACTG 
CCATTTTTGC CCACCCCTAT GGGGAAGGGT GTTGTCCCTG ACAACCATCC ATACTGTGTA 
GGTGCAGCCA GATCCAGGGC TTTGCAATTT GCTGATGTAA TTGTGTTATT TGGTGCCAGA 
CTAAATTGGA TTTTACATTT TGGACTGCCT CCAAGATATC AGCCAGATGT GAAGTTTATC 
CAGGTTGATA TCTGTGCAGA AGAATTGGGG AATAATGTAA AGCCCGCTGT TACTTTGCTA 
GGAAACATAC ATGCTGTCAC TAAGCAGCTT TTAGAGGAAC TTGATAAAAC ACCATGGCAG 
TATCCTCCAG AGAGCAAGTG GTGGAAAACT CTGAGAGAAA AAATGAAGAG CAATGAAGCT 
GCATCCAAGG AACTAGCTTC TAAAAAATCC CTGCCTATGA ATTATTACAC AGTATTCTAC 
CATGTTCAAG AACAACTACC TAGAGACTGT TTCGTGGTAA GTGAAGGAGC AAATACTATG 
GACATTGGAC GGACTGTGCT TCAGAACTAC CTTCCTCGTC ACAGGCTTGA TGCTGGTACT 
TTCGGAACAA TGGGAGTTGG TTTGGGATTT GCTATTGCAG CTGCCGTGGT GGCTAAAGAT 
AGAAGCCCTG GGCAATGGAT CATCTGTGTG GAAGGAGACA GTGCATTTGG GTTTTCTGGC 
ATGGAGGTAG AAACCATCTG CAGGTACAAC TTGCCAATCA TACTGTTGGT AGTGAATAAC 
AATGGAATTT ACCAAGGTTT TGATACAGAT ACTTGGAAAG AAATGTTAAA ATTTCAAGAT 
GCTACTGCAG TGGTCCCTCC AATGTGTTTG CTGCCAAATT CACATTATGA GCAAGTCATG 
ACTGCATTTG GAGGCAAAGG GTATTTTGTA CAAACACCAG AAGAACTCCA AAAATCCCTG 
AGGCAGAGCC TAGCAGACAC AACTAAACCT TCTCTTATCA ACATCATGAT TGAGCCACAA 
GCCACACGGA AGGCCCAGGA TTTTCATTGG CTGACCCGCT CTAATATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACL1 ( NM_001284413.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271342.1 Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu05364
Clone ID Related Accession (Same CDS sequence)	NM_001284413.1 , NM_001284413.2
Accession Version	NM_001284413.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-15
Organism	Homo sapiens(human)
Product	2-hydroxyacyl-CoA lyase 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB241165.1, DA609383.1, AK301546.1 and AA643224.1. On Oct 1, 2013 this sequence version replaced XM_005265038.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: AK301546.1, SRR1803614.233144.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2152474 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC CTTGCTTGTG 
ATTGGTGGTT CCTCTGAAAG AAACCAAGAA ACAATGGGAG CTTTCCAGGA GTTTCCTCAG 
GTTGAAGCTT GTAGATTATA TACCAAGTTC TCTGCCCGCC CAAGCAGCAT AGAAGCTATT 
CCTTTTGTTA TTGAAAAGGC AGTGAGAAGC AGTATCTATG GTCGTCCAGG TGCTTGCTAT 
GTTGACATAC CAGCAGATTT TGTGAACCTT CAGGTGAATG TGAATTCTAT AAAGTACATG 
GAACGCTGCA TGTCACCTCC TATTAGCATG GCAGAAACCT CTGCTGTGTG CACGGCGGCT 
TCTGTTATTA GGAATGCCAA ACAACCCCTT CTTATCATCG GGAAAGGTGC TGCTTACGCT 
CATGCAGAAG AGAGTATCAA GAAATTGGTG GAGCAATATA AACTGCCATT TTTGCCCACC 
CCTATGGGGA AGGGTGTTGT CCCTGACAAC CATCCATACT GTGTAGGTGC AGCCAGATCC 
AGGGCTTTGC AATTTGCTGA TGTAATTGTG TTATTTGGTG CCAGACTAAA TTGGATTTTA 
CATTTTGGAC TGCCTCCAAG ATATCAGCCA GATGTGAAGT TTATCCAGGT TGATATCTGT 
GCAGAAGAAT TGGGGAATAA TGTAAAGCCC GCTGTTACTT TGCTAGGAAA CATACATGCT 
GTCACTAAGC AGCTTTTAGA GGAACTTGAT AAAACACCAT GGCAGTATCC TCCAGAGAGC 
AAGTGGTGGA AAACTCTGAG AGAAAAAATG AAGAGCAATG AAGCTGCATC CAAGGAACTA 
GCTTCTAAAA AATCCCTGCC TATGAATTAT TACACAGTAT TCTACCATGT TCAAGAACAA 
CTACCTAGAG ACTGTTTCGT GGTAAGTGAA GGAGCAAATA CTATGGACAT TGGACGGACT 
GTGCTTCAGA ACTACCTTCC TCGTCACAGG CTTGATGCTG GTACTTTCGG AACAATGGGA 
GTTGGTTTGG GATTTGCTAT TGCAGCTGCC GTGGTGGCTA AAGATAGAAG CCCTGGGCAA 
TGGATCATCT GTGTGGAAGG AGACAGTGCA TTTGGGTTTT CTGGCATGGA GGTAGAAACC 
ATCTGCAGGT ACAACTTGCC AATCATACTG TTGGTAGTGA ATAACAATGG AATTTACCAA 
GGTTTTGATA CAGATACTTG GAAAGAAATG TTAAAATTTC AAGATGCTAC TGCAGTGGTC 
CCTCCAATGT GTTTGCTGCC AAATTCACAT TATGAGCAAG TCATGACTGC ATTTGGAGGC 
AAAGGGTATT TTGTACAAAC ACCAGAAGAA CTCCAAAAAT CCCTGAGGCA GAGCCTAGCA 
GACACAACTA AACCTTCTCT TATCAACATC ATGATTGAGC CACAAGCCAC ACGGAAGGCC 
CAGGATTTTC ATTGGCTGAC CCGCTCTAAT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271342.1
CDS	390..2045
Translation	MPDSNFAERSEEQVSGAKVIAQALKTQDVEYIFGIVGIPVTEIAIAAQQLGIKYIGMRNEQAACYAASAIGYLTSRPLLVIGGSSERNQETMGAFQEFPQVEACRLYTKFSARPSSIEAIPFVIEKAVRSSIYGRPGACYVDIPADFVNLQVNVNSIKYMERCMSPPISMAETSAVCTAASVIRNAKQPLLIIGKGAAYAHAEESIKKLVEQYKLPFLPTPMGKGVVPDNHPYCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQPDVKFIQVDICAEELGNNVKPAVTLLGNIHAVTKQLLEELDKTPWQYPPESKWWKTLREKMKSNEAASKELASKKSLPMNYYTVFYHVQEQLPRDCFVVSEGANTMDIGRTVLQNYLPRHRLDAGTFGTMGVGLGFAIAAAVVAKDRSPGQWIICVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKFQDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQKSLRQSLADTTKPSLINIMIEPQATRKAQDFHWLTRSNM

Target ORF information:

RefSeq Version	NM_001284413.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284413.1

ORF Insert Sequence:

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC CTTGCTTGTG 
ATTGGTGGTT CCTCTGAAAG AAACCAAGAA ACAATGGGAG CTTTCCAGGA GTTTCCTCAG 
GTTGAAGCTT GTAGATTATA TACCAAGTTC TCTGCCCGCC CAAGCAGCAT AGAAGCTATT 
CCTTTTGTTA TTGAAAAGGC AGTGAGAAGC AGTATCTATG GTCGTCCAGG TGCTTGCTAT 
GTTGACATAC CAGCAGATTT TGTGAACCTT CAGGTGAATG TGAATTCTAT AAAGTACATG 
GAACGCTGCA TGTCACCTCC TATTAGCATG GCAGAAACCT CTGCTGTGTG CACGGCGGCT 
TCTGTTATTA GGAATGCCAA ACAACCCCTT CTTATCATCG GGAAAGGTGC TGCTTACGCT 
CATGCAGAAG AGAGTATCAA GAAATTGGTG GAGCAATATA AACTGCCATT TTTGCCCACC 
CCTATGGGGA AGGGTGTTGT CCCTGACAAC CATCCATACT GTGTAGGTGC AGCCAGATCC 
AGGGCTTTGC AATTTGCTGA TGTAATTGTG TTATTTGGTG CCAGACTAAA TTGGATTTTA 
CATTTTGGAC TGCCTCCAAG ATATCAGCCA GATGTGAAGT TTATCCAGGT TGATATCTGT 
GCAGAAGAAT TGGGGAATAA TGTAAAGCCC GCTGTTACTT TGCTAGGAAA CATACATGCT 
GTCACTAAGC AGCTTTTAGA GGAACTTGAT AAAACACCAT GGCAGTATCC TCCAGAGAGC 
AAGTGGTGGA AAACTCTGAG AGAAAAAATG AAGAGCAATG AAGCTGCATC CAAGGAACTA 
GCTTCTAAAA AATCCCTGCC TATGAATTAT TACACAGTAT TCTACCATGT TCAAGAACAA 
CTACCTAGAG ACTGTTTCGT GGTAAGTGAA GGAGCAAATA CTATGGACAT TGGACGGACT 
GTGCTTCAGA ACTACCTTCC TCGTCACAGG CTTGATGCTG GTACTTTCGG AACAATGGGA 
GTTGGTTTGG GATTTGCTAT TGCAGCTGCC GTGGTGGCTA AAGATAGAAG CCCTGGGCAA 
TGGATCATCT GTGTGGAAGG AGACAGTGCA TTTGGGTTTT CTGGCATGGA GGTAGAAACC 
ATCTGCAGGT ACAACTTGCC AATCATACTG TTGGTAGTGA ATAACAATGG AATTTACCAA 
GGTTTTGATA CAGATACTTG GAAAGAAATG TTAAAATTTC AAGATGCTAC TGCAGTGGTC 
CCTCCAATGT GTTTGCTGCC AAATTCACAT TATGAGCAAG TCATGACTGC ATTTGGAGGC 
AAAGGGTATT TTGTACAAAC ACCAGAAGAA CTCCAAAAAT CCCTGAGGCA GAGCCTAGCA 
GACACAACTA AACCTTCTCT TATCAACATC ATGATTGAGC CACAAGCCAC ACGGAAGGCC 
CAGGATTTTC ATTGGCTGAC CCGCTCTAAT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACL1 ( NM_001284413.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271342.1 Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu05364
Clone ID Related Accession (Same CDS sequence)	NM_001284413.1 , NM_001284413.2
Accession Version	NM_001284413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	2-hydroxyacyl-CoA lyase 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA609383.1, AK301546.1, AA643224.1 and AC027129.6. On May 31, 2019 this sequence version replaced NM_001284413.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: AK301546.1, SRR1803617.37189.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2152474 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC CTTGCTTGTG 
ATTGGTGGTT CCTCTGAAAG AAACCAAGAA ACAATGGGAG CTTTCCAGGA GTTTCCTCAG 
GTTGAAGCTT GTAGATTATA TACCAAGTTC TCTGCCCGCC CAAGCAGCAT AGAAGCTATT 
CCTTTTGTTA TTGAAAAGGC AGTGAGAAGC AGTATCTATG GTCGTCCAGG TGCTTGCTAT 
GTTGACATAC CAGCAGATTT TGTGAACCTT CAGGTGAATG TGAATTCTAT AAAGTACATG 
GAACGCTGCA TGTCACCTCC TATTAGCATG GCAGAAACCT CTGCTGTGTG CACGGCGGCT 
TCTGTTATTA GGAATGCCAA ACAACCCCTT CTTATCATCG GGAAAGGTGC TGCTTACGCT 
CATGCAGAAG AGAGTATCAA GAAATTGGTG GAGCAATATA AACTGCCATT TTTGCCCACC 
CCTATGGGGA AGGGTGTTGT CCCTGACAAC CATCCATACT GTGTAGGTGC AGCCAGATCC 
AGGGCTTTGC AATTTGCTGA TGTAATTGTG TTATTTGGTG CCAGACTAAA TTGGATTTTA 
CATTTTGGAC TGCCTCCAAG ATATCAGCCA GATGTGAAGT TTATCCAGGT TGATATCTGT 
GCAGAAGAAT TGGGGAATAA TGTAAAGCCC GCTGTTACTT TGCTAGGAAA CATACATGCT 
GTCACTAAGC AGCTTTTAGA GGAACTTGAT AAAACACCAT GGCAGTATCC TCCAGAGAGC 
AAGTGGTGGA AAACTCTGAG AGAAAAAATG AAGAGCAATG AAGCTGCATC CAAGGAACTA 
GCTTCTAAAA AATCCCTGCC TATGAATTAT TACACAGTAT TCTACCATGT TCAAGAACAA 
CTACCTAGAG ACTGTTTCGT GGTAAGTGAA GGAGCAAATA CTATGGACAT TGGACGGACT 
GTGCTTCAGA ACTACCTTCC TCGTCACAGG CTTGATGCTG GTACTTTCGG AACAATGGGA 
GTTGGTTTGG GATTTGCTAT TGCAGCTGCC GTGGTGGCTA AAGATAGAAG CCCTGGGCAA 
TGGATCATCT GTGTGGAAGG AGACAGTGCA TTTGGGTTTT CTGGCATGGA GGTAGAAACC 
ATCTGCAGGT ACAACTTGCC AATCATACTG TTGGTAGTGA ATAACAATGG AATTTACCAA 
GGTTTTGATA CAGATACTTG GAAAGAAATG TTAAAATTTC AAGATGCTAC TGCAGTGGTC 
CCTCCAATGT GTTTGCTGCC AAATTCACAT TATGAGCAAG TCATGACTGC ATTTGGAGGC 
AAAGGGTATT TTGTACAAAC ACCAGAAGAA CTCCAAAAAT CCCTGAGGCA GAGCCTAGCA 
GACACAACTA AACCTTCTCT TATCAACATC ATGATTGAGC CACAAGCCAC ACGGAAGGCC 
CAGGATTTTC ATTGGCTGAC CCGCTCTAAT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271342.1
CDS	107..1762
Translation	MPDSNFAERSEEQVSGAKVIAQALKTQDVEYIFGIVGIPVTEIAIAAQQLGIKYIGMRNEQAACYAASAIGYLTSRPLLVIGGSSERNQETMGAFQEFPQVEACRLYTKFSARPSSIEAIPFVIEKAVRSSIYGRPGACYVDIPADFVNLQVNVNSIKYMERCMSPPISMAETSAVCTAASVIRNAKQPLLIIGKGAAYAHAEESIKKLVEQYKLPFLPTPMGKGVVPDNHPYCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQPDVKFIQVDICAEELGNNVKPAVTLLGNIHAVTKQLLEELDKTPWQYPPESKWWKTLREKMKSNEAASKELASKKSLPMNYYTVFYHVQEQLPRDCFVVSEGANTMDIGRTVLQNYLPRHRLDAGTFGTMGVGLGFAIAAAVVAKDRSPGQWIICVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKFQDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQKSLRQSLADTTKPSLINIMIEPQATRKAQDFHWLTRSNM

Target ORF information:

RefSeq Version	NM_001284413.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284413.2

ORF Insert Sequence:

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC CTTGCTTGTG 
ATTGGTGGTT CCTCTGAAAG AAACCAAGAA ACAATGGGAG CTTTCCAGGA GTTTCCTCAG 
GTTGAAGCTT GTAGATTATA TACCAAGTTC TCTGCCCGCC CAAGCAGCAT AGAAGCTATT 
CCTTTTGTTA TTGAAAAGGC AGTGAGAAGC AGTATCTATG GTCGTCCAGG TGCTTGCTAT 
GTTGACATAC CAGCAGATTT TGTGAACCTT CAGGTGAATG TGAATTCTAT AAAGTACATG 
GAACGCTGCA TGTCACCTCC TATTAGCATG GCAGAAACCT CTGCTGTGTG CACGGCGGCT 
TCTGTTATTA GGAATGCCAA ACAACCCCTT CTTATCATCG GGAAAGGTGC TGCTTACGCT 
CATGCAGAAG AGAGTATCAA GAAATTGGTG GAGCAATATA AACTGCCATT TTTGCCCACC 
CCTATGGGGA AGGGTGTTGT CCCTGACAAC CATCCATACT GTGTAGGTGC AGCCAGATCC 
AGGGCTTTGC AATTTGCTGA TGTAATTGTG TTATTTGGTG CCAGACTAAA TTGGATTTTA 
CATTTTGGAC TGCCTCCAAG ATATCAGCCA GATGTGAAGT TTATCCAGGT TGATATCTGT 
GCAGAAGAAT TGGGGAATAA TGTAAAGCCC GCTGTTACTT TGCTAGGAAA CATACATGCT 
GTCACTAAGC AGCTTTTAGA GGAACTTGAT AAAACACCAT GGCAGTATCC TCCAGAGAGC 
AAGTGGTGGA AAACTCTGAG AGAAAAAATG AAGAGCAATG AAGCTGCATC CAAGGAACTA 
GCTTCTAAAA AATCCCTGCC TATGAATTAT TACACAGTAT TCTACCATGT TCAAGAACAA 
CTACCTAGAG ACTGTTTCGT GGTAAGTGAA GGAGCAAATA CTATGGACAT TGGACGGACT 
GTGCTTCAGA ACTACCTTCC TCGTCACAGG CTTGATGCTG GTACTTTCGG AACAATGGGA 
GTTGGTTTGG GATTTGCTAT TGCAGCTGCC GTGGTGGCTA AAGATAGAAG CCCTGGGCAA 
TGGATCATCT GTGTGGAAGG AGACAGTGCA TTTGGGTTTT CTGGCATGGA GGTAGAAACC 
ATCTGCAGGT ACAACTTGCC AATCATACTG TTGGTAGTGA ATAACAATGG AATTTACCAA 
GGTTTTGATA CAGATACTTG GAAAGAAATG TTAAAATTTC AAGATGCTAC TGCAGTGGTC 
CCTCCAATGT GTTTGCTGCC AAATTCACAT TATGAGCAAG TCATGACTGC ATTTGGAGGC 
AAAGGGTATT TTGTACAAAC ACCAGAAGAA CTCCAAAAAT CCCTGAGGCA GAGCCTAGCA 
GACACAACTA AACCTTCTCT TATCAACATC ATGATTGAGC CACAAGCCAC ACGGAAGGCC 
CAGGATTTTC ATTGGCTGAC CCGCTCTAAT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACL1 ( NM_012260.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036392.2 Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 1, mRNA.

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CloneID	OHu06141
Clone ID Related Accession (Same CDS sequence)	NM_012260.3 , NM_012260.4
Accession Version	NM_012260.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	2-hydroxyacyl-CoA lyase 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB241165.1, DA609383.1, BC001627.1, AJ131753.1 and AA643224.1. On Oct 1, 2013 this sequence version replaced NM_012260.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1803611.96873.1, SRR1163657.387581.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2152474 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC AGGAGTCTGC 
CTTGTTGTTT CTGGCCCAGG TCTCATCCAT GCCTTGGGCG GTATGGCAAA TGCAAACATG 
AACTGCTGGC CCTTGCTTGT GATTGGTGGT TCCTCTGAAA GAAACCAAGA AACAATGGGA 
GCTTTCCAGG AGTTTCCTCA GGTTGAAGCT TGTAGATTAT ATACCAAGTT CTCTGCCCGC 
CCAAGCAGCA TAGAAGCTAT TCCTTTTGTT ATTGAAAAGG CAGTGAGAAG CAGTATCTAT 
GGTCGTCCAG GTGCTTGCTA TGTTGACATA CCAGCAGATT TTGTGAACCT TCAGGTGAAT 
GTGAATTCTA TAAAGTACAT GGAACGCTGC ATGTCACCTC CTATTAGCAT GGCAGAAACC 
TCTGCTGTGT GCACGGCGGC TTCTGTTATT AGGAATGCCA AACAACCCCT TCTTATCATC 
GGGAAAGGTG CTGCTTACGC TCATGCAGAA GAGAGTATCA AGAAATTGGT GGAGCAATAT 
AAACTGCCAT TTTTGCCCAC CCCTATGGGG AAGGGTGTTG TCCCTGACAA CCATCCATAC 
TGTGTAGGTG CAGCCAGATC CAGGGCTTTG CAATTTGCTG ATGTAATTGT GTTATTTGGT 
GCCAGACTAA ATTGGATTTT ACATTTTGGA CTGCCTCCAA GATATCAGCC AGATGTGAAG 
TTTATCCAGG TTGATATCTG TGCAGAAGAA TTGGGGAATA ATGTAAAGCC CGCTGTTACT 
TTGCTAGGAA ACATACATGC TGTCACTAAG CAGCTTTTAG AGGAACTTGA TAAAACACCA 
TGGCAGTATC CTCCAGAGAG CAAGTGGTGG AAAACTCTGA GAGAAAAAAT GAAGAGCAAT 
GAAGCTGCAT CCAAGGAACT AGCTTCTAAA AAATCCCTGC CTATGAATTA TTACACAGTA 
TTCTACCATG TTCAAGAACA ACTACCTAGA GACTGTTTCG TGGTAAGTGA AGGAGCAAAT 
ACTATGGACA TTGGACGGAC TGTGCTTCAG AACTACCTTC CTCGTCACAG GCTTGATGCT 
GGTACTTTCG GAACAATGGG AGTTGGTTTG GGATTTGCTA TTGCAGCTGC CGTGGTGGCT 
AAAGATAGAA GCCCTGGGCA ATGGATCATC TGTGTGGAAG GAGACAGTGC ATTTGGGTTT 
TCTGGCATGG AGGTAGAAAC CATCTGCAGG TACAACTTGC CAATCATACT GTTGGTAGTG 
AATAACAATG GAATTTACCA AGGTTTTGAT ACAGATACTT GGAAAGAAAT GTTAAAATTT 
CAAGATGCTA CTGCAGTGGT CCCTCCAATG TGTTTGCTGC CAAATTCACA TTATGAGCAA 
GTCATGACTG CATTTGGAGG CAAAGGGTAT TTTGTACAAA CACCAGAAGA ACTCCAAAAA 
TCCCTGAGGC AGAGCCTAGC AGACACAACT AAACCTTCTC TTATCAACAT CATGATTGAG 
CCACAAGCCA CACGGAAGGC CCAGGATTTT CATTGGCTGA CCCGCTCTAA TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036392.2
CDS	390..2126
Translation	MPDSNFAERSEEQVSGAKVIAQALKTQDVEYIFGIVGIPVTEIAIAAQQLGIKYIGMRNEQAACYAASAIGYLTSRPGVCLVVSGPGLIHALGGMANANMNCWPLLVIGGSSERNQETMGAFQEFPQVEACRLYTKFSARPSSIEAIPFVIEKAVRSSIYGRPGACYVDIPADFVNLQVNVNSIKYMERCMSPPISMAETSAVCTAASVIRNAKQPLLIIGKGAAYAHAEESIKKLVEQYKLPFLPTPMGKGVVPDNHPYCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQPDVKFIQVDICAEELGNNVKPAVTLLGNIHAVTKQLLEELDKTPWQYPPESKWWKTLREKMKSNEAASKELASKKSLPMNYYTVFYHVQEQLPRDCFVVSEGANTMDIGRTVLQNYLPRHRLDAGTFGTMGVGLGFAIAAAVVAKDRSPGQWIICVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKFQDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQKSLRQSLADTTKPSLINIMIEPQATRKAQDFHWLTRSNM

Target ORF information:

RefSeq Version	NM_012260.3
Organism	Homo sapiens(human)
Definition	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012260.3

ORF Insert Sequence:

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC AGGAGTCTGC 
CTTGTTGTTT CTGGCCCAGG TCTCATCCAT GCCTTGGGCG GTATGGCAAA TGCAAACATG 
AACTGCTGGC CCTTGCTTGT GATTGGTGGT TCCTCTGAAA GAAACCAAGA AACAATGGGA 
GCTTTCCAGG AGTTTCCTCA GGTTGAAGCT TGTAGATTAT ATACCAAGTT CTCTGCCCGC 
CCAAGCAGCA TAGAAGCTAT TCCTTTTGTT ATTGAAAAGG CAGTGAGAAG CAGTATCTAT 
GGTCGTCCAG GTGCTTGCTA TGTTGACATA CCAGCAGATT TTGTGAACCT TCAGGTGAAT 
GTGAATTCTA TAAAGTACAT GGAACGCTGC ATGTCACCTC CTATTAGCAT GGCAGAAACC 
TCTGCTGTGT GCACGGCGGC TTCTGTTATT AGGAATGCCA AACAACCCCT TCTTATCATC 
GGGAAAGGTG CTGCTTACGC TCATGCAGAA GAGAGTATCA AGAAATTGGT GGAGCAATAT 
AAACTGCCAT TTTTGCCCAC CCCTATGGGG AAGGGTGTTG TCCCTGACAA CCATCCATAC 
TGTGTAGGTG CAGCCAGATC CAGGGCTTTG CAATTTGCTG ATGTAATTGT GTTATTTGGT 
GCCAGACTAA ATTGGATTTT ACATTTTGGA CTGCCTCCAA GATATCAGCC AGATGTGAAG 
TTTATCCAGG TTGATATCTG TGCAGAAGAA TTGGGGAATA ATGTAAAGCC CGCTGTTACT 
TTGCTAGGAA ACATACATGC TGTCACTAAG CAGCTTTTAG AGGAACTTGA TAAAACACCA 
TGGCAGTATC CTCCAGAGAG CAAGTGGTGG AAAACTCTGA GAGAAAAAAT GAAGAGCAAT 
GAAGCTGCAT CCAAGGAACT AGCTTCTAAA AAATCCCTGC CTATGAATTA TTACACAGTA 
TTCTACCATG TTCAAGAACA ACTACCTAGA GACTGTTTCG TGGTAAGTGA AGGAGCAAAT 
ACTATGGACA TTGGACGGAC TGTGCTTCAG AACTACCTTC CTCGTCACAG GCTTGATGCT 
GGTACTTTCG GAACAATGGG AGTTGGTTTG GGATTTGCTA TTGCAGCTGC CGTGGTGGCT 
AAAGATAGAA GCCCTGGGCA ATGGATCATC TGTGTGGAAG GAGACAGTGC ATTTGGGTTT 
TCTGGCATGG AGGTAGAAAC CATCTGCAGG TACAACTTGC CAATCATACT GTTGGTAGTG 
AATAACAATG GAATTTACCA AGGTTTTGAT ACAGATACTT GGAAAGAAAT GTTAAAATTT 
CAAGATGCTA CTGCAGTGGT CCCTCCAATG TGTTTGCTGC CAAATTCACA TTATGAGCAA 
GTCATGACTG CATTTGGAGG CAAAGGGTAT TTTGTACAAA CACCAGAAGA ACTCCAAAAA 
TCCCTGAGGC AGAGCCTAGC AGACACAACT AAACCTTCTC TTATCAACAT CATGATTGAG 
CCACAAGCCA CACGGAAGGC CCAGGATTTT CATTGGCTGA CCCGCTCTAA TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HACL1 ( NM_012260.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036392.2 Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06141
Clone ID Related Accession (Same CDS sequence)	NM_012260.3 , NM_012260.4
Accession Version	NM_012260.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	2-hydroxyacyl-CoA lyase 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA609383.1, BC001627.1, AJ131753.1, AA643224.1 and AC027129.6. On May 17, 2019 this sequence version replaced NM_012260.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1803614.53132.1, SRR1803616.32893.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2152474 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000321169.10/ ENSP00000323811.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC AGGAGTCTGC 
CTTGTTGTTT CTGGCCCAGG TCTCATCCAT GCCTTGGGCG GTATGGCAAA TGCAAACATG 
AACTGCTGGC CCTTGCTTGT GATTGGTGGT TCCTCTGAAA GAAACCAAGA AACAATGGGA 
GCTTTCCAGG AGTTTCCTCA GGTTGAAGCT TGTAGATTAT ATACCAAGTT CTCTGCCCGC 
CCAAGCAGCA TAGAAGCTAT TCCTTTTGTT ATTGAAAAGG CAGTGAGAAG CAGTATCTAT 
GGTCGTCCAG GTGCTTGCTA TGTTGACATA CCAGCAGATT TTGTGAACCT TCAGGTGAAT 
GTGAATTCTA TAAAGTACAT GGAACGCTGC ATGTCACCTC CTATTAGCAT GGCAGAAACC 
TCTGCTGTGT GCACGGCGGC TTCTGTTATT AGGAATGCCA AACAACCCCT TCTTATCATC 
GGGAAAGGTG CTGCTTACGC TCATGCAGAA GAGAGTATCA AGAAATTGGT GGAGCAATAT 
AAACTGCCAT TTTTGCCCAC CCCTATGGGG AAGGGTGTTG TCCCTGACAA CCATCCATAC 
TGTGTAGGTG CAGCCAGATC CAGGGCTTTG CAATTTGCTG ATGTAATTGT GTTATTTGGT 
GCCAGACTAA ATTGGATTTT ACATTTTGGA CTGCCTCCAA GATATCAGCC AGATGTGAAG 
TTTATCCAGG TTGATATCTG TGCAGAAGAA TTGGGGAATA ATGTAAAGCC CGCTGTTACT 
TTGCTAGGAA ACATACATGC TGTCACTAAG CAGCTTTTAG AGGAACTTGA TAAAACACCA 
TGGCAGTATC CTCCAGAGAG CAAGTGGTGG AAAACTCTGA GAGAAAAAAT GAAGAGCAAT 
GAAGCTGCAT CCAAGGAACT AGCTTCTAAA AAATCCCTGC CTATGAATTA TTACACAGTA 
TTCTACCATG TTCAAGAACA ACTACCTAGA GACTGTTTCG TGGTAAGTGA AGGAGCAAAT 
ACTATGGACA TTGGACGGAC TGTGCTTCAG AACTACCTTC CTCGTCACAG GCTTGATGCT 
GGTACTTTCG GAACAATGGG AGTTGGTTTG GGATTTGCTA TTGCAGCTGC CGTGGTGGCT 
AAAGATAGAA GCCCTGGGCA ATGGATCATC TGTGTGGAAG GAGACAGTGC ATTTGGGTTT 
TCTGGCATGG AGGTAGAAAC CATCTGCAGG TACAACTTGC CAATCATACT GTTGGTAGTG 
AATAACAATG GAATTTACCA AGGTTTTGAT ACAGATACTT GGAAAGAAAT GTTAAAATTT 
CAAGATGCTA CTGCAGTGGT CCCTCCAATG TGTTTGCTGC CAAATTCACA TTATGAGCAA 
GTCATGACTG CATTTGGAGG CAAAGGGTAT TTTGTACAAA CACCAGAAGA ACTCCAAAAA 
TCCCTGAGGC AGAGCCTAGC AGACACAACT AAACCTTCTC TTATCAACAT CATGATTGAG 
CCACAAGCCA CACGGAAGGC CCAGGATTTT CATTGGCTGA CCCGCTCTAA TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036392.2
CDS	107..1843
Translation	MPDSNFAERSEEQVSGAKVIAQALKTQDVEYIFGIVGIPVTEIAIAAQQLGIKYIGMRNEQAACYAASAIGYLTSRPGVCLVVSGPGLIHALGGMANANMNCWPLLVIGGSSERNQETMGAFQEFPQVEACRLYTKFSARPSSIEAIPFVIEKAVRSSIYGRPGACYVDIPADFVNLQVNVNSIKYMERCMSPPISMAETSAVCTAASVIRNAKQPLLIIGKGAAYAHAEESIKKLVEQYKLPFLPTPMGKGVVPDNHPYCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQPDVKFIQVDICAEELGNNVKPAVTLLGNIHAVTKQLLEELDKTPWQYPPESKWWKTLREKMKSNEAASKELASKKSLPMNYYTVFYHVQEQLPRDCFVVSEGANTMDIGRTVLQNYLPRHRLDAGTFGTMGVGLGFAIAAAVVAKDRSPGQWIICVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKFQDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQKSLRQSLADTTKPSLINIMIEPQATRKAQDFHWLTRSNM

Target ORF information:

RefSeq Version	NM_012260.4
Organism	Homo sapiens(human)
Definition	Homo sapiens 2-hydroxyacyl-CoA lyase 1 (HACL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012260.4

ORF Insert Sequence:

ATGCCGGACA GTAACTTCGC AGAGCGCAGC GAGGAGCAGG TGTCTGGTGC TAAAGTCATC 
GCTCAGGCCC TGAAAACGCA AGATGTGGAG TACATATTTG GCATCGTAGG CATCCCAGTG 
ACCGAAATCG CCATTGCTGC CCAGCAGCTA GGCATCAAGT ACATCGGGAT GAGGAATGAG 
CAAGCGGCTT GTTATGCTGC CTCCGCGATT GGATATCTGA CAAGCAGGCC AGGAGTCTGC 
CTTGTTGTTT CTGGCCCAGG TCTCATCCAT GCCTTGGGCG GTATGGCAAA TGCAAACATG 
AACTGCTGGC CCTTGCTTGT GATTGGTGGT TCCTCTGAAA GAAACCAAGA AACAATGGGA 
GCTTTCCAGG AGTTTCCTCA GGTTGAAGCT TGTAGATTAT ATACCAAGTT CTCTGCCCGC 
CCAAGCAGCA TAGAAGCTAT TCCTTTTGTT ATTGAAAAGG CAGTGAGAAG CAGTATCTAT 
GGTCGTCCAG GTGCTTGCTA TGTTGACATA CCAGCAGATT TTGTGAACCT TCAGGTGAAT 
GTGAATTCTA TAAAGTACAT GGAACGCTGC ATGTCACCTC CTATTAGCAT GGCAGAAACC 
TCTGCTGTGT GCACGGCGGC TTCTGTTATT AGGAATGCCA AACAACCCCT TCTTATCATC 
GGGAAAGGTG CTGCTTACGC TCATGCAGAA GAGAGTATCA AGAAATTGGT GGAGCAATAT 
AAACTGCCAT TTTTGCCCAC CCCTATGGGG AAGGGTGTTG TCCCTGACAA CCATCCATAC 
TGTGTAGGTG CAGCCAGATC CAGGGCTTTG CAATTTGCTG ATGTAATTGT GTTATTTGGT 
GCCAGACTAA ATTGGATTTT ACATTTTGGA CTGCCTCCAA GATATCAGCC AGATGTGAAG 
TTTATCCAGG TTGATATCTG TGCAGAAGAA TTGGGGAATA ATGTAAAGCC CGCTGTTACT 
TTGCTAGGAA ACATACATGC TGTCACTAAG CAGCTTTTAG AGGAACTTGA TAAAACACCA 
TGGCAGTATC CTCCAGAGAG CAAGTGGTGG AAAACTCTGA GAGAAAAAAT GAAGAGCAAT 
GAAGCTGCAT CCAAGGAACT AGCTTCTAAA AAATCCCTGC CTATGAATTA TTACACAGTA 
TTCTACCATG TTCAAGAACA ACTACCTAGA GACTGTTTCG TGGTAAGTGA AGGAGCAAAT 
ACTATGGACA TTGGACGGAC TGTGCTTCAG AACTACCTTC CTCGTCACAG GCTTGATGCT 
GGTACTTTCG GAACAATGGG AGTTGGTTTG GGATTTGCTA TTGCAGCTGC CGTGGTGGCT 
AAAGATAGAA GCCCTGGGCA ATGGATCATC TGTGTGGAAG GAGACAGTGC ATTTGGGTTT 
TCTGGCATGG AGGTAGAAAC CATCTGCAGG TACAACTTGC CAATCATACT GTTGGTAGTG 
AATAACAATG GAATTTACCA AGGTTTTGAT ACAGATACTT GGAAAGAAAT GTTAAAATTT 
CAAGATGCTA CTGCAGTGGT CCCTCCAATG TGTTTGCTGC CAAATTCACA TTATGAGCAA 
GTCATGACTG CATTTGGAGG CAAAGGGTAT TTTGTACAAA CACCAGAAGA ACTCCAAAAA 
TCCCTGAGGC AGAGCCTAGC AGACACAACT AAACCTTCTC TTATCAACAT CATGATTGAG 
CCACAAGCCA CACGGAAGGC CCAGGATTTT CATTGGCTGA CCCGCTCTAA TATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An inter-species protein-protein interaction network across vast evolutionary distance.
Molecular systems biology12(4)865(2016 Apr)
Zhong Q,Pevzner SJ,Hao T,Wang Y,Mosca R,Menche J,Taipale M,Ta?an M,Fan C,Yang X,Haley P,Murray RR,Mer F,Gebreab F,Tam S,MacWilliams A,Dricot A,Reichert P,Santhanam B,Ghamsari L,Calderwood MA,Rolland T,Charloteaux B,Lindquist S,Barab?si AL,Hill DE,Aloy P,Cusick ME,Xia Y,Roth FP,Vidal M

RP-HPLC-fluorescence analysis of aliphatic aldehydes: application to aldehyde-generating enzymes HACL1 and SGPL1.
Journal of lipid research55(3)573-82(2014 Mar)
Mezzar S,de Schryver E,Van Veldhoven PP

Role of thiamine pyrophosphate in oligomerisation, functioning and import of peroxisomal 2-hydroxyacyl-CoA lyase.
Biochimica et biophysica acta1814(10)1226-33(2011 Oct)
Fraccascia P,Casteels M,De Schryver E,Van Veldhoven PP

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Journal of the American Chemical Society132(11)3973-9(2010 Mar)
Ghosh D,Berg JM

Biochemistry of mammalian peroxisomes revisited.
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Wanders RJ,Waterham HR

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HACL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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