PNKD cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PNKD
Entrez Gene ID	25953
Full Name	paroxysmal nonkinesigenic dyskinesia
Synonyms	BRP17,DYT8,FKSG19,FPD1,KIPP1184,MR-1,MR1,PDC,PKND1,PNKD1,TAHCCP2
General protein information	Preferred Names paroxysmal nonkinesigenic dyskinesia Names probable hydrolase PNKD brain protein 17 myofibrillogenesis regulator 1 trans-activated by hepatitis C virus core protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q35
Summary	This gene is thought to play a role in the regulation of myofibrillogenesis. Mutations in this gene have been associated with the movement disorder paroxysmal non-kinesigenic dyskinesia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010].
Disorder MIM:	609023
Disorder Html:	Paroxysmal nonkinesigenic dyskinesia 1, 118800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_015488.5	NP_056303.3	probable hydrolase PNKD isoform 1 precursor
NM_022572.4	NP_072094.1	probable hydrolase PNKD isoform 2
XM_017003772.1	XP_016859261.1	probable hydrolase PNKD isoform X2
NM_015488.4	NP_056303.3	probable hydrolase PNKD isoform 1 precursor
NM_001077399.3	NP_001070867.1	probable hydrolase PNKD isoform 3 precursor
NM_001077399.2	NP_001070867.1	probable hydrolase PNKD isoform 3 precursor
XM_017003771.1	XP_016859260.1	probable hydrolase PNKD isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PNKD	XP_853023.2
	PNKD	XP_001089254.1
Rattus norvegicus (Norway rat)	Pnkd	NP_001128222.1
Xenopus tropicalis (tropical clawed frog)	pnkd	NP_001106490.1
Homo sapiens (human)	PNKD	NP_056303.3
Gallus gallus (chicken)	PNKD	XP_004942937.1
Bos taurus (cattle)	PNKD	NP_001107642.1
Mus musculus (house mouse)	Pnkd	NP_001034598.1
Danio rerio (zebrafish)	pnkd	NP_001243139.1

Gene Ontology
Bibliography

Related articles in PubMed

The PNKD gene is associated with Tourette Disorder or Tic disorder in a multiplex family.
Sun N, Nasello C, Deng L, Wang N, Zhang Y, Xu Z, Song Z, Kwan K, King RA, Pang ZP, Xing J, Heiman GA, Tischfield JA
Molecular psychiatry(2017 Sep)

MR-1S Interacts with PET100 and PET117 in Module-Based Assembly of Human Cytochrome c Oxidase.
Vidoni S, Harbour ME, Guerrero-Castillo S, Signes A, Ding S, Fearnley IM, Taylor RW, Tiranti V, Arnold S, Fernandez-Vizarra E, Zeviani M
Cell reports18(7)1727-1738(2017 Feb)

Variation at 2q35 (PNKD and TMBIM1) influences colorectal cancer risk and identifies a pleiotropic effect with inflammatory bowel disease.
Orlando G, Law PJ, Palin K, Tuupanen S, Gylfe A, H?nninen UA, Cajuso T, Tanskanen T, Kondelin J, Kaasinen E, Sarin AP, Kaprio J, Eriksson JG, Rissanen H, Knekt P, Pukkala E, Jousilahti P, Salomaa V, Ripatti S, Palotie A, J?rvinen H, Renkonen-Sinisalo L, Lepist? A, B?hm J, Mecklin JP, Al-Tassan NA, Palles C, Martin L, Barclay E, Tenesa A, Farrington S, Timofeeva MN, Meyer BF, Wakil SM, Campbell H, Smith CG, Idziaszczyk S, Maughan TS, Kaplan R, Kerr R, Kerr D, Buchanan DD, Win AK, Hopper J, Jenkins M, Lindor NM, Newcomb PA, Gallinger S, Conti D, Schumacher F, Casey G, Taipale J, Cheadle JP, Dunlop MG, Tomlinson IP, Aaltonen LA, Houlston RS
Human molecular genetics25(11)2349-2359(2016 06)

The clinical and genetic heterogeneity of paroxysmal dyskinesias.
Gardiner AR, Jaffer F, Dale RC, Labrum R, Erro R, Meyer E, Xiromerisiou G, Stamelou M, Walker M, Kullmann D, Warner T, Jarman P, Hanna M, Kurian MA, Bhatia KP, Houlden H
Brain : a journal of neurology138(Pt 12)3567-80(2015 Dec)

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The short isoform of the myofibrillogenesis regulator 1 (MR-1S) as a new COX assembly factor, which works with the highly conserved PET100 and PET117 chaperones to assist COX biogenesis in higher eukaryotes.
Title: MR-1S Interacts with PET100 and PET117 in Module-Based Assembly of Human Cytochrome c Oxidase.

The combined analysis identified a new risk association for colorectal cancer (CRC) at 2q35 marked by rs992157 which is intronic to PNKD and TMBIM1.Intriguingly this susceptibility single-nucleotide polymorphism (SNP) is in strong linkage disequilibrium (r(2) = 0.90, D' = 0.96) with the previously discovered GWAS SNP rs2382817 for inflammatory bowel disease (IBD).
Title: Variation at 2q35 (PNKD and TMBIM1) influences colorectal cancer risk and identifies a pleiotropic effect with inflammatory bowel disease.

study highlights the frequency, novel mutations and clinical and molecular spectrum of PRRT2, SLC2A1 and PNKD mutations as well as the phenotype-genotype overlap among these paroxysmal movement disorders.
Title: The clinical and genetic heterogeneity of paroxysmal dyskinesias.

This study present the pedigree is the first PNKD family from Chinese Mainland, which is also the largest PNKD family among those reported across the globe. It included 5 generations and 26 patients.
Title: A case of familial paroxysmal nonkinesigenic dyskinesia due to mutation of the PNKD gene in Chinese Mainland.

MR-1 functions as a tumor promoter in MCF7 cells by activating the MEK/ERK signaling
Title: Phosphorylation of myofibrillogenesis regulator-1 activates the MAPK signaling pathway and induces proliferation and migration in human breast cancer MCF7 cells.

The following PNKD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PNKD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22569	NM_022572.4 Latest version!	Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$149.50-$209.30 $299.00
OHu27071	NM_001077399.3 Latest version!	Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu25801	NM_015488.5 Latest version!	Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu89247	XM_017003771.1 Latest version!	Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu89248	XM_017003772.1 Latest version!	Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu25801	NM_015488.4	Homo sapiens paroxysmal nonkinesigenic dyskinesia (PNKD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu27071	NM_001077399.2	Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PNKD ( NM_022572.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_072094.1 Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22569
Clone ID Related Accession (Same CDS sequence)	NM_022572.4
Accession Version	NM_022572.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	probable hydrolase PNKD isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU553179.1, BC002937.2, AC021016.5 and BC036457.2. On Mar 25, 2010 this sequence version replaced NM_022572.3. Transcript Variant: This variant (2), alternately referred to as the medium form (MR-1M), differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358753.1, BC002937.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGGC AGGGCTGGCC CGCGGCGTGG CAGTGGGTCG CCGGCTGCTG GCTCCTCCTC 
GTCCTTGTCC TCGTCCTACT TGTGAGCCCC CGCGGCTGCC GAGCGCGGCG GGGCCTCCGC 
GGTCTGCTCA TGGCGCACAG CCAGCGGCTG CTCTTCCGAA TCGGGTACAG CCTGTACACC 
CGCACCTGGC TCGGGTACCT CTTCTACCGA CAGCAGCTGC GCAGGGCTCG GAATCGCTAC 
CCTAAAGGCC ACTCGAAAAC CCAGCCCCGC CTCTTCAATG GAGTGAAGGT GCTTCCCATC 
CCTGTCCTCT CGGACAACTA CAGCTACCTC ATCATCGACA CCCAGGCCCA GCTGGCTGTG 
GCTGTGGACC CTTCAGACCC TCGGGCTGTG CAGGCTTCCA TTGAAAAGGA AGGGGTCACC 
TTGGTCGCCA TTCTGTGTAC TCACAAGCAC TGGGACCACA GTGGAGGGAA CCGTGACCTC 
AGCCGGCGGC ACCGGGACTG TCGGGTGTAC GGGAGCCCTC AGGACGGCAT CCCCTACCTC 
ACCCATCCCC TGTGTCATCA AGATGTGGTC AGCGTGGGAC GGCTTCAGAT CCGGGCCCTG 
GCTACACCTG GCCACACACA AGGCCATCTG GTCTACCTAC TGGATGGGGA GCCCTACAAG 
GGTCCCTCCT GCCTCTTCTC AGGGGACCTG CTCTTCCTCT CTGGCTGTGG GCGGACCTTT 
GAGGGCAATG CAGAGACCAT GCTGAGCTCA CTGGACACTG TGCTGGGGCT AGGGGATGAC 
ACCCTTCTGT GGCCTGGTCA TGAGTATGCA GAGGAGAACC TGGGCTTTGC AGGTGTGGTG 
GAGCCCGAGA ACCTGGCCCG GGAGAGGAAG ATGCAGTGGG TGCAGCGGCA GCGGCTGGAG 
CGCAAGGGCA CGTGCCCATC TACCCTGGGA GAGGAGCGCT CCTACAACCC GTTCCTGAGA 
ACCCACTGCC TGGCGCTACA GGAGGCTCTG GGGCCGGGGC CGGGCCCCAC TGGGGATGAT 
GACTACTCCC GGGCCCAGCT CCTGGAAGAG CTCCGCCGGC TGAAGGATAT GCACAAGAGC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072094.1
CDS	90..1175
Translation	MAWQGWPAAWQWVAGCWLLLVLVLVLLVSPRGCRARRGLRGLLMAHSQRLLFRIGYSLYTRTWLGYLFYRQQLRRARNRYPKGHSKTQPRLFNGVKVLPIPVLSDNYSYLIIDTQAQLAVAVDPSDPRAVQASIEKEGVTLVAILCTHKHWDHSGGNRDLSRRHRDCRVYGSPQDGIPYLTHPLCHQDVVSVGRLQIRALATPGHTQGHLVYLLDGEPYKGPSCLFSGDLLFLSGCGRTFEGNAETMLSSLDTVLGLGDDTLLWPGHEYAEENLGFAGVVEPENLARERKMQWVQRQRLERKGTCPSTLGEERSYNPFLRTHCLALQEALGPGPGPTGDDDYSRAQLLEELRRLKDMHKSK

Target ORF information:

RefSeq Version	NM_022572.4
Organism	Homo sapiens(human)
Definition	Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022572.4

ORF Insert Sequence:

ATGGCTTGGC AGGGCTGGCC CGCGGCGTGG CAGTGGGTCG CCGGCTGCTG GCTCCTCCTC 
GTCCTTGTCC TCGTCCTACT TGTGAGCCCC CGCGGCTGCC GAGCGCGGCG GGGCCTCCGC 
GGTCTGCTCA TGGCGCACAG CCAGCGGCTG CTCTTCCGAA TCGGGTACAG CCTGTACACC 
CGCACCTGGC TCGGGTACCT CTTCTACCGA CAGCAGCTGC GCAGGGCTCG GAATCGCTAC 
CCTAAAGGCC ACTCGAAAAC CCAGCCCCGC CTCTTCAATG GAGTGAAGGT GCTTCCCATC 
CCTGTCCTCT CGGACAACTA CAGCTACCTC ATCATCGACA CCCAGGCCCA GCTGGCTGTG 
GCTGTGGACC CTTCAGACCC TCGGGCTGTG CAGGCTTCCA TTGAAAAGGA AGGGGTCACC 
TTGGTCGCCA TTCTGTGTAC TCACAAGCAC TGGGACCACA GTGGAGGGAA CCGTGACCTC 
AGCCGGCGGC ACCGGGACTG TCGGGTGTAC GGGAGCCCTC AGGACGGCAT CCCCTACCTC 
ACCCATCCCC TGTGTCATCA AGATGTGGTC AGCGTGGGAC GGCTTCAGAT CCGGGCCCTG 
GCTACACCTG GCCACACACA AGGCCATCTG GTCTACCTAC TGGATGGGGA GCCCTACAAG 
GGTCCCTCCT GCCTCTTCTC AGGGGACCTG CTCTTCCTCT CTGGCTGTGG GCGGACCTTT 
GAGGGCAATG CAGAGACCAT GCTGAGCTCA CTGGACACTG TGCTGGGGCT AGGGGATGAC 
ACCCTTCTGT GGCCTGGTCA TGAGTATGCA GAGGAGAACC TGGGCTTTGC AGGTGTGGTG 
GAGCCCGAGA ACCTGGCCCG GGAGAGGAAG ATGCAGTGGG TGCAGCGGCA GCGGCTGGAG 
CGCAAGGGCA CGTGCCCATC TACCCTGGGA GAGGAGCGCT CCTACAACCC GTTCCTGAGA 
ACCCACTGCC TGGCGCTACA GGAGGCTCTG GGGCCGGGGC CGGGCCCCAC TGGGGATGAT 
GACTACTCCC GGGCCCAGCT CCTGGAAGAG CTCCGCCGGC TGAAGGATAT GCACAAGAGC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNKD ( NM_001077399.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001070867.1 Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27071
Clone ID Related Accession (Same CDS sequence)	NM_001077399.3 , NM_001077399.2
Accession Version	NM_001077399.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	probable hydrolase PNKD isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF318057.1 and BQ028777.1. On May 31, 2019 this sequence version replaced NM_001077399.2. Transcript Variant: This variant (3), alternately referred to as the short form (MR-1S), differs in the 3' UTR and has multiple coding region differences, compared to variant 1. This results in a frameshift in isoform 3, which has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189652.245422.1, SRR7410570.213015.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TGGTAGCTGC TACGGCGCTG AAGGGCCGGG GGGCGAGAAA TGCCCGCGTC 
CTCCGGGGGA TTCTCGCAGG AGCCACAGCT AACAAGGCTT CTCATAACAG GACCCGGGCC 
CTGCAAAGCC ACAGCTCCCC AGAGGGCAAG GAGGAACCTG AACCCCTATC CCCGGAGCTG 
GAATACATTC CCAGAAAGAG GGGCAAGAAC CCCATGAAAG CTGTGGGACT GGCCTGGGCC 
ATCGGCTTCC CTTGTGGTAT CCTCCTCTTC ATCCTCACCA AGCGGGAAGT GGACAAGGAC 
CGTGTGAAGC AGATGAAGGC TCGGCAGAAC ATGCGGTTGT CCAACACGGG CGAGTATGAG 
AGCCAGAGGT TCAGGGCTTC CTCCCAGAGT GCCCCGTCCC CTGATGTTGG GTCTGGGGTG 
CAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070867.1
CDS	18..446
Translation	MAAVVAATALKGRGARNARVLRGILAGATANKASHNRTRALQSHSSPEGKEEPEPLSPELEYIPRKRGKNPMKAVGLAWAIGFPCGILLFILTKREVDKDRVKQMKARQNMRLSNTGEYESQRFRASSQSAPSPDVGSGVQT

Target ORF information:

RefSeq Version	NM_001077399.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077399.3

ORF Insert Sequence:

ATGGCGGCGG TGGTAGCTGC TACGGCGCTG AAGGGCCGGG GGGCGAGAAA TGCCCGCGTC 
CTCCGGGGGA TTCTCGCAGG AGCCACAGCT AACAAGGCTT CTCATAACAG GACCCGGGCC 
CTGCAAAGCC ACAGCTCCCC AGAGGGCAAG GAGGAACCTG AACCCCTATC CCCGGAGCTG 
GAATACATTC CCAGAAAGAG GGGCAAGAAC CCCATGAAAG CTGTGGGACT GGCCTGGGCC 
ATCGGCTTCC CTTGTGGTAT CCTCCTCTTC ATCCTCACCA AGCGGGAAGT GGACAAGGAC 
CGTGTGAAGC AGATGAAGGC TCGGCAGAAC ATGCGGTTGT CCAACACGGG CGAGTATGAG 
AGCCAGAGGT TCAGGGCTTC CTCCCAGAGT GCCCCGTCCC CTGATGTTGG GTCTGGGGTG 
CAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNKD ( NM_001077399.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001070867.1 Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27071
Clone ID Related Accession (Same CDS sequence)	NM_001077399.3 , NM_001077399.2
Accession Version	NM_001077399.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	probable hydrolase PNKD isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF318057.1 and BQ028777.1. On Mar 25, 2010 this sequence version replaced NM_001077399.1. Transcript Variant: This variant (3), alternately referred to as the short form (MR-1S), differs in the 3' UTR and has multiple coding region differences, compared to variant 1. This results in a frameshift in isoform 3, which has a distinct C-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF318057.1, SRR1163657.565051.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TGGTAGCTGC TACGGCGCTG AAGGGCCGGG GGGCGAGAAA TGCCCGCGTC 
CTCCGGGGGA TTCTCGCAGG AGCCACAGCT AACAAGGCTT CTCATAACAG GACCCGGGCC 
CTGCAAAGCC ACAGCTCCCC AGAGGGCAAG GAGGAACCTG AACCCCTATC CCCGGAGCTG 
GAATACATTC CCAGAAAGAG GGGCAAGAAC CCCATGAAAG CTGTGGGACT GGCCTGGGCC 
ATCGGCTTCC CTTGTGGTAT CCTCCTCTTC ATCCTCACCA AGCGGGAAGT GGACAAGGAC 
CGTGTGAAGC AGATGAAGGC TCGGCAGAAC ATGCGGTTGT CCAACACGGG CGAGTATGAG 
AGCCAGAGGT TCAGGGCTTC CTCCCAGAGT GCCCCGTCCC CTGATGTTGG GTCTGGGGTG 
CAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070867.1
CDS	145..573
Translation	MAAVVAATALKGRGARNARVLRGILAGATANKASHNRTRALQSHSSPEGKEEPEPLSPELEYIPRKRGKNPMKAVGLAWAIGFPCGILLFILTKREVDKDRVKQMKARQNMRLSNTGEYESQRFRASSQSAPSPDVGSGVQT

Target ORF information:

RefSeq Version	NM_001077399.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077399.2

ORF Insert Sequence:

ATGGCGGCGG TGGTAGCTGC TACGGCGCTG AAGGGCCGGG GGGCGAGAAA TGCCCGCGTC 
CTCCGGGGGA TTCTCGCAGG AGCCACAGCT AACAAGGCTT CTCATAACAG GACCCGGGCC 
CTGCAAAGCC ACAGCTCCCC AGAGGGCAAG GAGGAACCTG AACCCCTATC CCCGGAGCTG 
GAATACATTC CCAGAAAGAG GGGCAAGAAC CCCATGAAAG CTGTGGGACT GGCCTGGGCC 
ATCGGCTTCC CTTGTGGTAT CCTCCTCTTC ATCCTCACCA AGCGGGAAGT GGACAAGGAC 
CGTGTGAAGC AGATGAAGGC TCGGCAGAAC ATGCGGTTGT CCAACACGGG CGAGTATGAG 
AGCCAGAGGT TCAGGGCTTC CTCCCAGAGT GCCCCGTCCC CTGATGTTGG GTCTGGGGTG 
CAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNKD ( NM_015488.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_056303.3 Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25801
Clone ID Related Accession (Same CDS sequence)	NM_015488.5 , NM_015488.4
Accession Version	NM_015488.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	probable hydrolase PNKD isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF318057.1, AB033010.1, BC002937.2 and BC036457.2. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_015488.4. Transcript Variant: This variant (1), alternately referred to as the long form (MR-1L), represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036457.2, SRR1660803.2910.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000273077.9/ ENSP00000273077.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGG TGGTAGCTGC TACGGCGCTG AAGGGCCGGG GGGCGAGAAA TGCCCGCGTC 
CTCCGGGGGA TTCTCGCAGG AGCCACAGCT AACAAGGCTT CTCATAACAG GACCCGGGCC 
CTGCAAAGCC ACAGCTCCCC AGAGGGCAAG GAGGAACCTG AACCCCTATC CCCGGAGCTG 
GAATACATTC CCAGAAAGAG GGGCAAGAAC CCCATGAAAG CTGTGGGACT GGCCTGGTAC 
AGCCTGTACA CCCGCACCTG GCTCGGGTAC CTCTTCTACC GACAGCAGCT GCGCAGGGCT 
CGGAATCGCT ACCCTAAAGG CCACTCGAAA ACCCAGCCCC GCCTCTTCAA TGGAGTGAAG 
GTGCTTCCCA TCCCTGTCCT CTCGGACAAC TACAGCTACC TCATCATCGA CACCCAGGCC 
CAGCTGGCTG TGGCTGTGGA CCCTTCAGAC CCTCGGGCTG TGCAGGCTTC CATTGAAAAG 
GAAGGGGTCA CCTTGGTCGC CATTCTGTGT ACTCACAAGC ACTGGGACCA CAGTGGAGGG 
AACCGTGACC TCAGCCGGCG GCACCGGGAC TGTCGGGTGT ACGGGAGCCC TCAGGACGGC 
ATCCCCTACC TCACCCATCC CCTGTGTCAT CAAGATGTGG TCAGCGTGGG ACGGCTTCAG 
ATCCGGGCCC TGGCTACACC TGGCCACACA CAAGGCCATC TGGTCTACCT ACTGGATGGG 
GAGCCCTACA AGGGTCCCTC CTGCCTCTTC TCAGGGGACC TGCTCTTCCT CTCTGGCTGT 
GGGCGGACCT TTGAGGGCAA TGCAGAGACC ATGCTGAGCT CACTGGACAC TGTGCTGGGG 
CTAGGGGATG ACACCCTTCT GTGGCCTGGT CATGAGTATG CAGAGGAGAA CCTGGGCTTT 
GCAGGTGTGG TGGAGCCCGA GAACCTGGCC CGGGAGAGGA AGATGCAGTG GGTGCAGCGG 
CAGCGGCTGG AGCGCAAGGG CACGTGCCCA TCTACCCTGG GAGAGGAGCG CTCCTACAAC 
CCGTTCCTGA GAACCCACTG CCTGGCGCTA CAGGAGGCTC TGGGGCCGGG GCCGGGCCCC 
ACTGGGGATG ATGACTACTC CCGGGCCCAG CTCCTGGAAG AGCTCCGCCG GCTGAAGGAT 
ATGCACAAGA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056303.3
CDS	18..1175
Translation	MAAVVAATALKGRGARNARVLRGILAGATANKASHNRTRALQSHSSPEGKEEPEPLSPELEYIPRKRGKNPMKAVGLAWYSLYTRTWLGYLFYRQQLRRARNRYPKGHSKTQPRLFNGVKVLPIPVLSDNYSYLIIDTQAQLAVAVDPSDPRAVQASIEKEGVTLVAILCTHKHWDHSGGNRDLSRRHRDCRVYGSPQDGIPYLTHPLCHQDVVSVGRLQIRALATPGHTQGHLVYLLDGEPYKGPSCLFSGDLLFLSGCGRTFEGNAETMLSSLDTVLGLGDDTLLWPGHEYAEENLGFAGVVEPENLARERKMQWVQRQRLERKGTCPSTLGEERSYNPFLRTHCLALQEALGPGPGPTGDDDYSRAQLLEELRRLKDMHKSK

Target ORF information:

RefSeq Version	NM_015488.5
Organism	Homo sapiens(human)
Definition	Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015488.5

ORF Insert Sequence:

ATGGCGGCGG TGGTAGCTGC TACGGCGCTG AAGGGCCGGG GGGCGAGAAA TGCCCGCGTC 
CTCCGGGGGA TTCTCGCAGG AGCCACAGCT AACAAGGCTT CTCATAACAG GACCCGGGCC 
CTGCAAAGCC ACAGCTCCCC AGAGGGCAAG GAGGAACCTG AACCCCTATC CCCGGAGCTG 
GAATACATTC CCAGAAAGAG GGGCAAGAAC CCCATGAAAG CTGTGGGACT GGCCTGGTAC 
AGCCTGTACA CCCGCACCTG GCTCGGGTAC CTCTTCTACC GACAGCAGCT GCGCAGGGCT 
CGGAATCGCT ACCCTAAAGG CCACTCGAAA ACCCAGCCCC GCCTCTTCAA TGGAGTGAAG 
GTGCTTCCCA TCCCTGTCCT CTCGGACAAC TACAGCTACC TCATCATCGA CACCCAGGCC 
CAGCTGGCTG TGGCTGTGGA CCCTTCAGAC CCTCGGGCTG TGCAGGCTTC CATTGAAAAG 
GAAGGGGTCA CCTTGGTCGC CATTCTGTGT ACTCACAAGC ACTGGGACCA CAGTGGAGGG 
AACCGTGACC TCAGCCGGCG GCACCGGGAC TGTCGGGTGT ACGGGAGCCC TCAGGACGGC 
ATCCCCTACC TCACCCATCC CCTGTGTCAT CAAGATGTGG TCAGCGTGGG ACGGCTTCAG 
ATCCGGGCCC TGGCTACACC TGGCCACACA CAAGGCCATC TGGTCTACCT ACTGGATGGG 
GAGCCCTACA AGGGTCCCTC CTGCCTCTTC TCAGGGGACC TGCTCTTCCT CTCTGGCTGT 
GGGCGGACCT TTGAGGGCAA TGCAGAGACC ATGCTGAGCT CACTGGACAC TGTGCTGGGG 
CTAGGGGATG ACACCCTTCT GTGGCCTGGT CATGAGTATG CAGAGGAGAA CCTGGGCTTT 
GCAGGTGTGG TGGAGCCCGA GAACCTGGCC CGGGAGAGGA AGATGCAGTG GGTGCAGCGG 
CAGCGGCTGG AGCGCAAGGG CACGTGCCCA TCTACCCTGG GAGAGGAGCG CTCCTACAAC 
CCGTTCCTGA GAACCCACTG CCTGGCGCTA CAGGAGGCTC TGGGGCCGGG GCCGGGCCCC 
ACTGGGGATG ATGACTACTC CCGGGCCCAG CTCCTGGAAG AGCTCCGCCG GCTGAAGGAT 
ATGCACAAGA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNKD ( NM_015488.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056303.3 Homo sapiens paroxysmal nonkinesigenic dyskinesia (PNKD), transcript variant 1, mRNA.

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CloneID	OHu25801
Clone ID Related Accession (Same CDS sequence)	NM_015488.5 , NM_015488.4
Accession Version	NM_015488.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	probable hydrolase PNKD isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF318057.1, AB033010.1, BC002937.2 and BC036457.2. This sequence is a reference standard in the RefSeqGene project. On Oct 25, 2006 this sequence version replaced NM_015488.3. Transcript Variant: This variant (1), alternately referred to as the long form (MR-1L), represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036457.2, SRR1803612.224547.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TGGTAGCTGC TACGGCGCTG AAGGGCCGGG GGGCGAGAAA TGCCCGCGTC 
CTCCGGGGGA TTCTCGCAGG AGCCACAGCT AACAAGGCTT CTCATAACAG GACCCGGGCC 
CTGCAAAGCC ACAGCTCCCC AGAGGGCAAG GAGGAACCTG AACCCCTATC CCCGGAGCTG 
GAATACATTC CCAGAAAGAG GGGCAAGAAC CCCATGAAAG CTGTGGGACT GGCCTGGTAC 
AGCCTGTACA CCCGCACCTG GCTCGGGTAC CTCTTCTACC GACAGCAGCT GCGCAGGGCT 
CGGAATCGCT ACCCTAAAGG CCACTCGAAA ACCCAGCCCC GCCTCTTCAA TGGAGTGAAG 
GTGCTTCCCA TCCCTGTCCT CTCGGACAAC TACAGCTACC TCATCATCGA CACCCAGGCC 
CAGCTGGCTG TGGCTGTGGA CCCTTCAGAC CCTCGGGCTG TGCAGGCTTC CATTGAAAAG 
GAAGGGGTCA CCTTGGTCGC CATTCTGTGT ACTCACAAGC ACTGGGACCA CAGTGGAGGG 
AACCGTGACC TCAGCCGGCG GCACCGGGAC TGTCGGGTGT ACGGGAGCCC TCAGGACGGC 
ATCCCCTACC TCACCCATCC CCTGTGTCAT CAAGATGTGG TCAGCGTGGG ACGGCTTCAG 
ATCCGGGCCC TGGCTACACC TGGCCACACA CAAGGCCATC TGGTCTACCT ACTGGATGGG 
GAGCCCTACA AGGGTCCCTC CTGCCTCTTC TCAGGGGACC TGCTCTTCCT CTCTGGCTGT 
GGGCGGACCT TTGAGGGCAA TGCAGAGACC ATGCTGAGCT CACTGGACAC TGTGCTGGGG 
CTAGGGGATG ACACCCTTCT GTGGCCTGGT CATGAGTATG CAGAGGAGAA CCTGGGCTTT 
GCAGGTGTGG TGGAGCCCGA GAACCTGGCC CGGGAGAGGA AGATGCAGTG GGTGCAGCGG 
CAGCGGCTGG AGCGCAAGGG CACGTGCCCA TCTACCCTGG GAGAGGAGCG CTCCTACAAC 
CCGTTCCTGA GAACCCACTG CCTGGCGCTA CAGGAGGCTC TGGGGCCGGG GCCGGGCCCC 
ACTGGGGATG ATGACTACTC CCGGGCCCAG CTCCTGGAAG AGCTCCGCCG GCTGAAGGAT 
ATGCACAAGA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056303.3
CDS	145..1302
Translation	MAAVVAATALKGRGARNARVLRGILAGATANKASHNRTRALQSHSSPEGKEEPEPLSPELEYIPRKRGKNPMKAVGLAWYSLYTRTWLGYLFYRQQLRRARNRYPKGHSKTQPRLFNGVKVLPIPVLSDNYSYLIIDTQAQLAVAVDPSDPRAVQASIEKEGVTLVAILCTHKHWDHSGGNRDLSRRHRDCRVYGSPQDGIPYLTHPLCHQDVVSVGRLQIRALATPGHTQGHLVYLLDGEPYKGPSCLFSGDLLFLSGCGRTFEGNAETMLSSLDTVLGLGDDTLLWPGHEYAEENLGFAGVVEPENLARERKMQWVQRQRLERKGTCPSTLGEERSYNPFLRTHCLALQEALGPGPGPTGDDDYSRAQLLEELRRLKDMHKSK

Target ORF information:

RefSeq Version	NM_015488.4
Organism	Homo sapiens(human)
Definition	Homo sapiens paroxysmal nonkinesigenic dyskinesia (PNKD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015488.4

ORF Insert Sequence:

ATGGCGGCGG TGGTAGCTGC TACGGCGCTG AAGGGCCGGG GGGCGAGAAA TGCCCGCGTC 
CTCCGGGGGA TTCTCGCAGG AGCCACAGCT AACAAGGCTT CTCATAACAG GACCCGGGCC 
CTGCAAAGCC ACAGCTCCCC AGAGGGCAAG GAGGAACCTG AACCCCTATC CCCGGAGCTG 
GAATACATTC CCAGAAAGAG GGGCAAGAAC CCCATGAAAG CTGTGGGACT GGCCTGGTAC 
AGCCTGTACA CCCGCACCTG GCTCGGGTAC CTCTTCTACC GACAGCAGCT GCGCAGGGCT 
CGGAATCGCT ACCCTAAAGG CCACTCGAAA ACCCAGCCCC GCCTCTTCAA TGGAGTGAAG 
GTGCTTCCCA TCCCTGTCCT CTCGGACAAC TACAGCTACC TCATCATCGA CACCCAGGCC 
CAGCTGGCTG TGGCTGTGGA CCCTTCAGAC CCTCGGGCTG TGCAGGCTTC CATTGAAAAG 
GAAGGGGTCA CCTTGGTCGC CATTCTGTGT ACTCACAAGC ACTGGGACCA CAGTGGAGGG 
AACCGTGACC TCAGCCGGCG GCACCGGGAC TGTCGGGTGT ACGGGAGCCC TCAGGACGGC 
ATCCCCTACC TCACCCATCC CCTGTGTCAT CAAGATGTGG TCAGCGTGGG ACGGCTTCAG 
ATCCGGGCCC TGGCTACACC TGGCCACACA CAAGGCCATC TGGTCTACCT ACTGGATGGG 
GAGCCCTACA AGGGTCCCTC CTGCCTCTTC TCAGGGGACC TGCTCTTCCT CTCTGGCTGT 
GGGCGGACCT TTGAGGGCAA TGCAGAGACC ATGCTGAGCT CACTGGACAC TGTGCTGGGG 
CTAGGGGATG ACACCCTTCT GTGGCCTGGT CATGAGTATG CAGAGGAGAA CCTGGGCTTT 
GCAGGTGTGG TGGAGCCCGA GAACCTGGCC CGGGAGAGGA AGATGCAGTG GGTGCAGCGG 
CAGCGGCTGG AGCGCAAGGG CACGTGCCCA TCTACCCTGG GAGAGGAGCG CTCCTACAAC 
CCGTTCCTGA GAACCCACTG CCTGGCGCTA CAGGAGGCTC TGGGGCCGGG GCCGGGCCCC 
ACTGGGGATG ATGACTACTC CCGGGCCCAG CTCCTGGAAG AGCTCCGCCG GCTGAAGGAT 
ATGCACAAGA GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNKD ( XM_017003771.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859260.1 Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu89247
Clone ID Related Accession (Same CDS sequence)	XM_017003771.1
Accession Version	XM_017003771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable hydrolase PNKD isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG TGGTAGCTGC TACGGCGCTG AAGGGCCGGG GGGCGAGAAA TGCCCGCGTC 
CTCCGGGGGA TTCTCGCAGG AGCCACAGCT AACAAGGCTT CTCATAACAG GACCCGGGCC 
CTGCAAAGCC ACAGCTCCCC AGAGGGCAAG GAGGAACCTG AACCCCTATC CCCGGAGCTG 
GAATACATTC CCAGAAAGAG GGGCAAGAAC CCCATGAAAG CTGTGGGACT GGCCTGGTAC 
AGCCTGTACA CCCGCACCTG GCTCGGGTAC CTCTTCTACC GACAGCAGCT GCGCAGGGCT 
CGGAATCGCT ACCCTAAAGG CCACTCGAAA ACCCAGCCCC GCCTCTTCAA TGGAGTGAAG 
GTGCTTCCCA TCCCTGTCCT CTCGGACAAC TACAGCTACC TCATCATCGA CACCCAGGCC 
CAGCTGGCTG TGGCTGTGGA CCCTTCAGAC CCTCGGGCTG TGCAGGCTTC CATTGAAAAG 
GAAGGGGTCA CCTTGGTCGC CATTCTGTGT ACTCACAAGC ACTGGGACCA CAGTGGAGGG 
AACCGTGACC TCAGCCGGCG GCACCGGGAC TGTCGGGTGT ACGGGAGCCC TCAGGACGGC 
ATCCCCTACC TCACCCATCC CCTGTGTCAT CAAGATGTGG TCAGCGTGGG ACGGCTTCAG 
ATCCGGGCCC TGGCTACACC TGGCCACACA CAAGGCCATC TGGTCTACCT ACTGGATGGG 
GAGCCCTACA AGGGTCCCTC CTGCCTCTTC TCAGGGGACC TGCTCTTCCT CTCTGGCTGT 
GGTCATGAGT ATGCAGAGGA GAACCTGGGC TTTGCAGGTG TGGTGGAGCC CGAGAACCTG 
GCCCGGGAGA GGAAGATGCA GTGGGTGCAG CGGCAGCGGC TGGAGCGCAA GGGCACGTGC 
CCATCTACCC TGGGAGAGGA GCGCTCCTAC AACCCGTTCC TGAGAACCCA CTGCCTGGCG 
CTACAGGAGG CTCTGGGGCC GGGGCCGGGC CCCACTGGGG ATGATGACTA CTCCCGGGCC 
CAGCTCCTGG AAGAGCTCCG CCGGCTGAAG GATATGCACA AGAGCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859260.1
CDS	52..1122
Translation	MAAVVAATALKGRGARNARVLRGILAGATANKASHNRTRALQSHSSPEGKEEPEPLSPELEYIPRKRGKNPMKAVGLAWYSLYTRTWLGYLFYRQQLRRARNRYPKGHSKTQPRLFNGVKVLPIPVLSDNYSYLIIDTQAQLAVAVDPSDPRAVQASIEKEGVTLVAILCTHKHWDHSGGNRDLSRRHRDCRVYGSPQDGIPYLTHPLCHQDVVSVGRLQIRALATPGHTQGHLVYLLDGEPYKGPSCLFSGDLLFLSGCGHEYAEENLGFAGVVEPENLARERKMQWVQRQRLERKGTCPSTLGEERSYNPFLRTHCLALQEALGPGPGPTGDDDYSRAQLLEELRRLKDMHKSK

Target ORF information:

RefSeq Version	XM_017003771.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003771.1

ORF Insert Sequence:

ATGGCGGCGG TGGTAGCTGC TACGGCGCTG AAGGGCCGGG GGGCGAGAAA TGCCCGCGTC 
CTCCGGGGGA TTCTCGCAGG AGCCACAGCT AACAAGGCTT CTCATAACAG GACCCGGGCC 
CTGCAAAGCC ACAGCTCCCC AGAGGGCAAG GAGGAACCTG AACCCCTATC CCCGGAGCTG 
GAATACATTC CCAGAAAGAG GGGCAAGAAC CCCATGAAAG CTGTGGGACT GGCCTGGTAC 
AGCCTGTACA CCCGCACCTG GCTCGGGTAC CTCTTCTACC GACAGCAGCT GCGCAGGGCT 
CGGAATCGCT ACCCTAAAGG CCACTCGAAA ACCCAGCCCC GCCTCTTCAA TGGAGTGAAG 
GTGCTTCCCA TCCCTGTCCT CTCGGACAAC TACAGCTACC TCATCATCGA CACCCAGGCC 
CAGCTGGCTG TGGCTGTGGA CCCTTCAGAC CCTCGGGCTG TGCAGGCTTC CATTGAAAAG 
GAAGGGGTCA CCTTGGTCGC CATTCTGTGT ACTCACAAGC ACTGGGACCA CAGTGGAGGG 
AACCGTGACC TCAGCCGGCG GCACCGGGAC TGTCGGGTGT ACGGGAGCCC TCAGGACGGC 
ATCCCCTACC TCACCCATCC CCTGTGTCAT CAAGATGTGG TCAGCGTGGG ACGGCTTCAG 
ATCCGGGCCC TGGCTACACC TGGCCACACA CAAGGCCATC TGGTCTACCT ACTGGATGGG 
GAGCCCTACA AGGGTCCCTC CTGCCTCTTC TCAGGGGACC TGCTCTTCCT CTCTGGCTGT 
GGTCATGAGT ATGCAGAGGA GAACCTGGGC TTTGCAGGTG TGGTGGAGCC CGAGAACCTG 
GCCCGGGAGA GGAAGATGCA GTGGGTGCAG CGGCAGCGGC TGGAGCGCAA GGGCACGTGC 
CCATCTACCC TGGGAGAGGA GCGCTCCTAC AACCCGTTCC TGAGAACCCA CTGCCTGGCG 
CTACAGGAGG CTCTGGGGCC GGGGCCGGGC CCCACTGGGG ATGATGACTA CTCCCGGGCC 
CAGCTCCTGG AAGAGCTCCG CCGGCTGAAG GATATGCACA AGAGCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNKD ( XM_017003772.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859261.1 Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu89248
Clone ID Related Accession (Same CDS sequence)	XM_017003772.1
Accession Version	XM_017003772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable hydrolase PNKD isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGGC AGGGCTGGCC CGCGGCGTGG CAGTGGGTCG CCGGCTGCTG GCTCCTCCTC 
GTCCTTGTCC TCGTCCTACT TGTGAGCCCC CGCGGCTGCC GAGCGCGGCG GGGCCTCCGC 
GGTCTGCTCA TGGCGCACAG CCAGCGGCTG CTCTTCCGAA TCGGGTACAG CCTGTACACC 
CGCACCTGGC TCGGGTACCT CTTCTACCGA CAGCAGCTGC GCAGGGCTCG GAATCGCTAC 
CCTAAAGGCC ACTCGAAAAC CCAGCCCCGC CTCTTCAATG GAGTGAAGGT GCTTCCCATC 
CCTGTCCTCT CGGACAACTA CAGCTACCTC ATCATCGACA CCCAGGCCCA GCTGGCTGTG 
GCTGTGGACC CTTCAGACCC TCGGGCTGTG CAGGCTTCCA TTGAAAAGGA AGGGGTCACC 
TTGGTCGCCA TTCTGTGTAC TCACAAGCAC TGGGACCACA GTGGAGGGAA CCGTGACCTC 
AGCCGGCGGC ACCGGGACTG TCGGGTGTAC GGGAGCCCTC AGGACGGCAT CCCCTACCTC 
ACCCATCCCC TGTGTCATCA AGATGTGGTC AGCGTGGGAC GGCTTCAGAT CCGGGCCCTG 
GCTACACCTG GCCACACACA AGGCCATCTG GTCTACCTAC TGGATGGGGA GCCCTACAAG 
GGTCCCTCCT GCCTCTTCTC AGGGGACCTG CTCTTCCTCT CTGGCTGTGG TCATGAGTAT 
GCAGAGGAGA ACCTGGGCTT TGCAGGTGTG GTGGAGCCCG AGAACCTGGC CCGGGAGAGG 
AAGATGCAGT GGGTGCAGCG GCAGCGGCTG GAGCGCAAGG GCACGTGCCC ATCTACCCTG 
GGAGAGGAGC GCTCCTACAA CCCGTTCCTG AGAACCCACT GCCTGGCGCT ACAGGAGGCT 
CTGGGGCCGG GGCCGGGCCC CACTGGGGAT GATGACTACT CCCGGGCCCA GCTCCTGGAA 
GAGCTCCGCC GGCTGAAGGA TATGCACAAG AGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859261.1
CDS	115..1113
Translation	MAWQGWPAAWQWVAGCWLLLVLVLVLLVSPRGCRARRGLRGLLMAHSQRLLFRIGYSLYTRTWLGYLFYRQQLRRARNRYPKGHSKTQPRLFNGVKVLPIPVLSDNYSYLIIDTQAQLAVAVDPSDPRAVQASIEKEGVTLVAILCTHKHWDHSGGNRDLSRRHRDCRVYGSPQDGIPYLTHPLCHQDVVSVGRLQIRALATPGHTQGHLVYLLDGEPYKGPSCLFSGDLLFLSGCGHEYAEENLGFAGVVEPENLARERKMQWVQRQRLERKGTCPSTLGEERSYNPFLRTHCLALQEALGPGPGPTGDDDYSRAQLLEELRRLKDMHKSK

Target ORF information:

RefSeq Version	XM_017003772.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PNKD metallo-beta-lactamase domain containing (PNKD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017003772.1

ORF Insert Sequence:

ATGGCTTGGC AGGGCTGGCC CGCGGCGTGG CAGTGGGTCG CCGGCTGCTG GCTCCTCCTC 
GTCCTTGTCC TCGTCCTACT TGTGAGCCCC CGCGGCTGCC GAGCGCGGCG GGGCCTCCGC 
GGTCTGCTCA TGGCGCACAG CCAGCGGCTG CTCTTCCGAA TCGGGTACAG CCTGTACACC 
CGCACCTGGC TCGGGTACCT CTTCTACCGA CAGCAGCTGC GCAGGGCTCG GAATCGCTAC 
CCTAAAGGCC ACTCGAAAAC CCAGCCCCGC CTCTTCAATG GAGTGAAGGT GCTTCCCATC 
CCTGTCCTCT CGGACAACTA CAGCTACCTC ATCATCGACA CCCAGGCCCA GCTGGCTGTG 
GCTGTGGACC CTTCAGACCC TCGGGCTGTG CAGGCTTCCA TTGAAAAGGA AGGGGTCACC 
TTGGTCGCCA TTCTGTGTAC TCACAAGCAC TGGGACCACA GTGGAGGGAA CCGTGACCTC 
AGCCGGCGGC ACCGGGACTG TCGGGTGTAC GGGAGCCCTC AGGACGGCAT CCCCTACCTC 
ACCCATCCCC TGTGTCATCA AGATGTGGTC AGCGTGGGAC GGCTTCAGAT CCGGGCCCTG 
GCTACACCTG GCCACACACA AGGCCATCTG GTCTACCTAC TGGATGGGGA GCCCTACAAG 
GGTCCCTCCT GCCTCTTCTC AGGGGACCTG CTCTTCCTCT CTGGCTGTGG TCATGAGTAT 
GCAGAGGAGA ACCTGGGCTT TGCAGGTGTG GTGGAGCCCG AGAACCTGGC CCGGGAGAGG 
AAGATGCAGT GGGTGCAGCG GCAGCGGCTG GAGCGCAAGG GCACGTGCCC ATCTACCCTG 
GGAGAGGAGC GCTCCTACAA CCCGTTCCTG AGAACCCACT GCCTGGCGCT ACAGGAGGCT 
CTGGGGCCGG GGCCGGGCCC CACTGGGGAT GATGACTACT CCCGGGCCCA GCTCCTGGAA 
GAGCTCCGCC GGCTGAAGGA TATGCACAAG AGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The PNKD gene is associated with Tourette Disorder or Tic disorder in a multiplex family.
Molecular psychiatry(2017 Sep)
Sun N,Nasello C,Deng L,Wang N,Zhang Y,Xu Z,Song Z,Kwan K,King RA,Pang ZP,Xing J,Heiman GA,Tischfield JA

MR-1S Interacts with PET100 and PET117 in Module-Based Assembly of Human Cytochrome c Oxidase.
Cell reports18(7)1727-1738(2017 Feb)
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Variation at 2q35 (PNKD and TMBIM1) influences colorectal cancer risk and identifies a pleiotropic effect with inflammatory bowel disease.
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Orlando G,Law PJ,Palin K,Tuupanen S,Gylfe A,H?nninen UA,Cajuso T,Tanskanen T,Kondelin J,Kaasinen E,Sarin AP,Kaprio J,Eriksson JG,Rissanen H,Knekt P,Pukkala E,Jousilahti P,Salomaa V,Ripatti S,Palotie A,J?rvinen H,Renkonen-Sinisalo L,Lepist? A,B?hm J,Mecklin JP,Al-Tassan NA,Palles C,Martin L,Barclay E,Tenesa A,Farrington S,Timofeeva MN,Meyer BF,Wakil SM,Campbell H,Smith CG,Idziaszczyk S,Maughan TS,Kaplan R,Kerr R,Kerr D,Buchanan DD,Win AK,Hopper J,Jenkins M,Lindor NM,Newcomb PA,Gallinger S,Conti D,Schumacher F,Casey G,Taipale J,Cheadle JP,Dunlop MG,Tomlinson IP,Aaltonen LA,Houlston RS

The clinical and genetic heterogeneity of paroxysmal dyskinesias.
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PNKD cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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