ATP6V0A2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP6V0A2
Entrez Gene ID	23545
Full Name	ATPase H+ transporting V0 subunit a2
Synonyms	A2,ARCL,ARCL2A,ATP6A2,ATP6N1D,J6B7,RTF,STV1,TJ6,TJ6M,TJ6S,VPH1,WSS
General protein information	Preferred Names ATPase H+ transporting V0 subunit a2 Names V-type proton ATPase 116 kDa subunit a isoform 2 A2V-ATPase ATPase, H+ transporting, lysosomal V0 subunit a2 lysosomal H(+)-transporting ATPase V0 subunit a2 regeneration and tolerance factor v-ATPase 116 kDa v-type proton ATPase 116 kDa subunit a vacuolar proton translocating ATPase 116 kDa subunit a
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.31
Summary	The protein encoded by this gene is a subunit of the vacuolar ATPase (v-ATPase), an heteromultimeric enzyme that is present in intracellular vesicles and in the plasma membrane of specialized cells, and which is essential for the acidification of diverse cellular components. V-ATPase is comprised of a membrane peripheral V(1) domain for ATP hydrolysis, and an integral membrane V(0) domain for proton translocation. The subunit encoded by this gene is a component of the V(0) domain. Mutations in this gene are a cause of both cutis laxa type II and wrinkly skin syndrome. [provided by RefSeq, Jul 2009].
Disorder MIM:	611716
Disorder Html:	Cutis laxa, autosomal recessive, type IIA, 219200 (3); Wrinkly skin syndrome, 278250 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024448911.1	XP_024304679.1	V-type proton ATPase 116 kDa subunit a isoform X2
NM_012463.4	NP_036595.2	V-type proton ATPase 116 kDa subunit a isoform 2
XM_024448910.1	XP_024304678.1	V-type proton ATPase 116 kDa subunit a isoform X1
NM_012463.3	NP_036595.2	V-type proton ATPase 116 kDa subunit a isoform 2
XM_024448912.1	XP_024304680.1	V-type proton ATPase 116 kDa subunit a isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP6V0A2	XP_543370.2
Bos taurus (cattle)	ATP6V0A2	NP_788810.1
Rattus norvegicus (Norway rat)	Atp6v0a2	NP_446227.2
Pan troglodytes (chimpanzee)	ATP6V0A2	XP_003314058.1
Homo sapiens (human)	ATP6V0A2	NP_036595.2
Mus musculus (house mouse)	Atp6v0a2	NP_035726.2
Gallus gallus (chicken)	ATP6V0A2	NP_990054.1
Danio rerio (zebrafish)	atp6v0a2a	XP_708125.5
Xenopus tropicalis (tropical clawed frog)	atp6v0a2	NP_001016223.1
Danio rerio (zebrafish)	atp6v0a2b	NP_001116219.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular mechanisms of cutis laxa and distal renal tubular acidosis-causing mutations in V-ATPase
Esmail S, Kartner N, Yao Y, Kim JW, Reithmeier RAF, Manolson MF
The Journal of biological chemistry(2018 Jan)

The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels.
Miles AL, Burr SP, Grice GL, Nathan JA
eLife6(2017 03)

Cancer derived peptide of vacuolar ATPase 'a2' isoform promotes neutrophil migration by autocrine secretion of IL-8.
Ibrahim SA, Kulshrestha A, Katara GK, Amin MA, Beaman KD
Scientific reports636865(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a2V deficiency disrupts the endolysosomal route in Notch and TGF signaling, thereby impairing mammary gland development.
Title: The V-ATPase a2 isoform controls mammary gland development through Notch and TGF-? signaling.

In cisplatin resistant cells, shRNA mediated inhibition of V-ATPase-V0a2 enhanced sensitivity towards both cisplatin and carboplatin.
Title: Selective inhibition of tumor cell associated Vacuolar-ATPase 'a2' isoform overcomes cisplatin resistance in ovarian cancer cells.

Senescence-associated impaired expression of ATP6V0A2 triggers changes in Golgi structure and glycosylation in old fibroblasts, which demonstrates a role of ATP6V0A2 in cellular senescence program.
Title: Impaired ATP6V0A2 expression contributes to Golgi dispersion and glycosylation changes in senescent cells.

the results from this study demonstrate that the a2-subunit isoform of Vacuolar ATPase regulates Notch signaling in breast tumor cells
Title: The Vacuolar ATPase a2-subunit regulates Notch signaling in triple-negative breast cancer cells.

The granule-associated a2V isoform has a role in maintaining a pH gradient within the cell between the cytosol and granules in neutrophils.
Title: Expression and role of a2 vacuolar-ATPase (a2V) in trafficking of human neutrophil granules and exocytosis.

The following ATP6V0A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP6V0A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22501	NM_012463.4 Latest version!	Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu46448	XM_024448910.1 Latest version!	Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu46449	XM_024448911.1 Latest version!	Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu46450	XM_024448912.1 Latest version!	Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu22501	NM_012463.3	Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP6V0A2 ( NM_012463.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036595.2 Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), mRNA.

Price & Availability↑

CloneID	OHu22501
Clone ID Related Accession (Same CDS sequence)	NM_012463.4 , NM_012463.3
Accession Version	NM_012463.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	V-type proton ATPase 116 kDa subunit a isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC068531.1, DA737084.1, BI562012.1, AC117503.8 and R51339.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_012463.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.270118.1, SRR1660805.19163.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000330342.8/ ENSP00000332247.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGTCCC TGTTCCGGAG CGAGACCATG TGCCTGGCGC AGCTCTTCCT GCAGTCGGGC 
ACGGCCTACG AGTGCCTCAG CGCCCTGGGC GAGAAAGGCC TGGTCCAGTT CCGAGACCTC 
AACCAGAACG TAAGTTCTTT CCAAAGAAAA TTTGTTGGTG AGGTGAAGAG GTGTGAAGAG 
CTAGAGCGAA TATTGGTGTA TTTGGTACAG GAAATTAATA GAGCTGATAT TCCCCTTCCT 
GAAGGAGAGG CCAGCCCTCC TGCGCCACCC CTGAAACAGG TTCTAGAAAT GCAGGAGCAG 
TTGCAGAAGC TCGAGGTTGA ACTGAGAGAA GTCACTAAGA ACAAGGAGAA ACTGAGGAAA 
AACTTGCTGG AACTGATAGA GTACACTCAC ATGCTGAGAG TGACAAAGAC CTTTGTGAAA 
CGCAATGTTG AGTTTGAACC CACTTATGAA GAATTCCCTT CCTTAGAGAG TGATTCTTTG 
TTGGATTACA GCTGTATGCA GAGGCTGGGA GCAAAACTGG GATTTGTGTC TGGCCTAATT 
AACCAAGGAA AAGTGGAAGC ATTTGAAAAA ATGTTGTGGA GAGTCTGCAA AGGGTACACC 
ATCGTGTCCT ATGCAGAACT GGATGAATCC CTTGAAGACC CTGAAACAGG GGAAGTCATA 
AAATGGTATG TCTTTTTAAT ATCCTTTTGG GGAGAGCAGA TTGGCCACAA GGTTAAGAAG 
ATATGTGATT GCTACCACTG CCACGTGTAC CCCTATCCAA ACACAGCCGA GGAGCGGAGG 
GAGATCCAGG AGGGGCTGAA CACCCGCATC CAGGATCTCT ACACTGTACT GCACAAAACC 
GAGGACTATT TGAGGCAAGT GCTATGTAAA GCCGCCGAGT CTGTCTACAG CCGTGTGATC 
CAGGTGAAGA AAATGAAGGC CATCTATCAC ATGCTGAACA TGTGCAGCTT TGACGTGACC 
AACAAGTGCC TCATTGCTGA GGTCTGGTGT CCCGAGGCGG ATCTGCAGGA CCTGCGCCGG 
GCACTGGAGG AGGGCTCGAG AGAGAGTGGT GCTACAATCC CCTCATTCAT GAATATAATC 
CCCACAAAAG AAACACCCCC CACTCGGATC CGCACCAACA AATTCACCGA GGGATTTCAG 
AACATCGTGG ATGCTTATGG AGTCGGAAGC TACAGAGAAG TCAATCCAGC TCTCTTTACC 
ATCATCACCT TCCCGTTTTT ATTTGCTGTG ATGTTTGGAG ACTTCGGACA TGGCTTTGTG 
ATGTTTTTAT TTGCCCTCTT GTTGGTGTTA AATGAAAATC ATCCCAGACT AAATCAGTCA 
CAAGAGATCA TGAGGATGTT TTTTAATGGC CGGTACATCC TCCTGCTGAT GGGGCTGTTC 
TCAGTGTACA CTGGCCTCAT CTACAACGAC TGCTTTTCAA AGTCAGTCAA CCTGTTCGGC 
TCTGGGTGGA ACGTGTCGGC CATGTACAGC TCCAGCCACC CACCCGCAGA GCATAAGAAG 
ATGGTGCTTT GGAATGACAG CGTCGTTAGA CACAACAGCA TTTTGCAGCT GGATCCAAGC 
ATTCCTGGAG TGTTCCGAGG CCCTTATCCC CTTGGCATTG ATCCTATTTG GAACTTGGCC 
ACAAATCGCC TCACTTTTCT AAACTCTTTC AAAATGAAAA TGTCCGTGAT TTTAGGAATC 
ATTCATATGA CTTTTGGAGT CATTCTGGGA ATATTTAACC ACTTGCACTT CAGGAAGAAG 
TTCAACATTT ACCTGGTTTC CATCCCGGAA CTTCTCTTCA TGCTCTGTAT CTTTGGATAC 
CTTATATTTA TGATTTTCTA CAAGTGGCTG GTTTTTTCAG CAGAAACCTC CAGAGTTGCT 
CCCAGCATTC TGATTGAATT TATTAACATG TTTTTATTCC CAGCCAGTAA AACAAGTGGC 
CTTTACACAG GGCAGGAGTA TGTCCAGAGA GTGCTGCTGG TTGTCACAGC ATTGTCTGTC 
CCTGTCCTCT TCTTGGGAAA GCCACTGTTT TTGTTGTGGC TTCACAATGG GCGTAGTTGC 
TTCGGGGTGA ACCGGAGTGG CTACACACTT ATAAGGAAAG ATAGTGAGGA AGAAGTTTCA 
TTGCTGGGAA GCCAAGATAT AGAAGAGGGA AATCACCAGG TGGAAGATGG ATGTAGAGAA 
ATGGCGTGTG AAGAGTTTAA TTTTGGAGAA ATATTAATGA CCCAAGTAAT CCATTCCATC 
GAGTACTGTC TGGGATGCAT CTCCAACACC GCCTCCTACC TGAGGCTCTG GGCGCTTAGC 
CTGGCTCACG CACAGTTGTC TGATGTCCTG TGGGCCATGC TGATGCGCGT GGGCCTCCGC 
GTTGACACCA CCTATGGCGT CTTGCTACTG CTCCCGGTTA TCGCGCTCTT TGCAGTTTTG 
ACCATTTTCA TCCTTCTGAT CATGGAAGGG CTTTCTGCGT TTCTTCACGC CATACGCCTC 
CACTGGGTAG AATTTCAGAA CAAATTCTAC GTTGGTGCAG GCACCAAATT TGTTCCTTTC 
TCATTCAGTC TACTTTCATC AAAGTTCAAT AACGACGACA GTGTGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036595.2
CDS	214..2784
Translation	MGSLFRSETMCLAQLFLQSGTAYECLSALGEKGLVQFRDLNQNVSSFQRKFVGEVKRCEELERILVYLVQEINRADIPLPEGEASPPAPPLKQVLEMQEQLQKLEVELREVTKNKEKLRKNLLELIEYTHMLRVTKTFVKRNVEFEPTYEEFPSLESDSLLDYSCMQRLGAKLGFVSGLINQGKVEAFEKMLWRVCKGYTIVSYAELDESLEDPETGEVIKWYVFLISFWGEQIGHKVKKICDCYHCHVYPYPNTAEERREIQEGLNTRIQDLYTVLHKTEDYLRQVLCKAAESVYSRVIQVKKMKAIYHMLNMCSFDVTNKCLIAEVWCPEADLQDLRRALEEGSRESGATIPSFMNIIPTKETPPTRIRTNKFTEGFQNIVDAYGVGSYREVNPALFTIITFPFLFAVMFGDFGHGFVMFLFALLLVLNENHPRLNQSQEIMRMFFNGRYILLLMGLFSVYTGLIYNDCFSKSVNLFGSGWNVSAMYSSSHPPAEHKKMVLWNDSVVRHNSILQLDPSIPGVFRGPYPLGIDPIWNLATNRLTFLNSFKMKMSVILGIIHMTFGVILGIFNHLHFRKKFNIYLVSIPELLFMLCIFGYLIFMIFYKWLVFSAETSRVAPSILIEFINMFLFPASKTSGLYTGQEYVQRVLLVVTALSVPVLFLGKPLFLLWLHNGRSCFGVNRSGYTLIRKDSEEEVSLLGSQDIEEGNHQVEDGCREMACEEFNFGEILMTQVIHSIEYCLGCISNTASYLRLWALSLAHAQLSDVLWAMLMRVGLRVDTTYGVLLLLPVIALFAVLTIFILLIMEGLSAFLHAIRLHWVEFQNKFYVGAGTKFVPFSFSLLSSKFNNDDSVA

Target ORF information:

RefSeq Version	NM_012463.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012463.4

ORF Insert Sequence:

ATGGGGTCCC TGTTCCGGAG CGAGACCATG TGCCTGGCGC AGCTCTTCCT GCAGTCGGGC 
ACGGCCTACG AGTGCCTCAG CGCCCTGGGC GAGAAAGGCC TGGTCCAGTT CCGAGACCTC 
AACCAGAACG TAAGTTCTTT CCAAAGAAAA TTTGTTGGTG AGGTGAAGAG GTGTGAAGAG 
CTAGAGCGAA TATTGGTGTA TTTGGTACAG GAAATTAATA GAGCTGATAT TCCCCTTCCT 
GAAGGAGAGG CCAGCCCTCC TGCGCCACCC CTGAAACAGG TTCTAGAAAT GCAGGAGCAG 
TTGCAGAAGC TCGAGGTTGA ACTGAGAGAA GTCACTAAGA ACAAGGAGAA ACTGAGGAAA 
AACTTGCTGG AACTGATAGA GTACACTCAC ATGCTGAGAG TGACAAAGAC CTTTGTGAAA 
CGCAATGTTG AGTTTGAACC CACTTATGAA GAATTCCCTT CCTTAGAGAG TGATTCTTTG 
TTGGATTACA GCTGTATGCA GAGGCTGGGA GCAAAACTGG GATTTGTGTC TGGCCTAATT 
AACCAAGGAA AAGTGGAAGC ATTTGAAAAA ATGTTGTGGA GAGTCTGCAA AGGGTACACC 
ATCGTGTCCT ATGCAGAACT GGATGAATCC CTTGAAGACC CTGAAACAGG GGAAGTCATA 
AAATGGTATG TCTTTTTAAT ATCCTTTTGG GGAGAGCAGA TTGGCCACAA GGTTAAGAAG 
ATATGTGATT GCTACCACTG CCACGTGTAC CCCTATCCAA ACACAGCCGA GGAGCGGAGG 
GAGATCCAGG AGGGGCTGAA CACCCGCATC CAGGATCTCT ACACTGTACT GCACAAAACC 
GAGGACTATT TGAGGCAAGT GCTATGTAAA GCCGCCGAGT CTGTCTACAG CCGTGTGATC 
CAGGTGAAGA AAATGAAGGC CATCTATCAC ATGCTGAACA TGTGCAGCTT TGACGTGACC 
AACAAGTGCC TCATTGCTGA GGTCTGGTGT CCCGAGGCGG ATCTGCAGGA CCTGCGCCGG 
GCACTGGAGG AGGGCTCGAG AGAGAGTGGT GCTACAATCC CCTCATTCAT GAATATAATC 
CCCACAAAAG AAACACCCCC CACTCGGATC CGCACCAACA AATTCACCGA GGGATTTCAG 
AACATCGTGG ATGCTTATGG AGTCGGAAGC TACAGAGAAG TCAATCCAGC TCTCTTTACC 
ATCATCACCT TCCCGTTTTT ATTTGCTGTG ATGTTTGGAG ACTTCGGACA TGGCTTTGTG 
ATGTTTTTAT TTGCCCTCTT GTTGGTGTTA AATGAAAATC ATCCCAGACT AAATCAGTCA 
CAAGAGATCA TGAGGATGTT TTTTAATGGC CGGTACATCC TCCTGCTGAT GGGGCTGTTC 
TCAGTGTACA CTGGCCTCAT CTACAACGAC TGCTTTTCAA AGTCAGTCAA CCTGTTCGGC 
TCTGGGTGGA ACGTGTCGGC CATGTACAGC TCCAGCCACC CACCCGCAGA GCATAAGAAG 
ATGGTGCTTT GGAATGACAG CGTCGTTAGA CACAACAGCA TTTTGCAGCT GGATCCAAGC 
ATTCCTGGAG TGTTCCGAGG CCCTTATCCC CTTGGCATTG ATCCTATTTG GAACTTGGCC 
ACAAATCGCC TCACTTTTCT AAACTCTTTC AAAATGAAAA TGTCCGTGAT TTTAGGAATC 
ATTCATATGA CTTTTGGAGT CATTCTGGGA ATATTTAACC ACTTGCACTT CAGGAAGAAG 
TTCAACATTT ACCTGGTTTC CATCCCGGAA CTTCTCTTCA TGCTCTGTAT CTTTGGATAC 
CTTATATTTA TGATTTTCTA CAAGTGGCTG GTTTTTTCAG CAGAAACCTC CAGAGTTGCT 
CCCAGCATTC TGATTGAATT TATTAACATG TTTTTATTCC CAGCCAGTAA AACAAGTGGC 
CTTTACACAG GGCAGGAGTA TGTCCAGAGA GTGCTGCTGG TTGTCACAGC ATTGTCTGTC 
CCTGTCCTCT TCTTGGGAAA GCCACTGTTT TTGTTGTGGC TTCACAATGG GCGTAGTTGC 
TTCGGGGTGA ACCGGAGTGG CTACACACTT ATAAGGAAAG ATAGTGAGGA AGAAGTTTCA 
TTGCTGGGAA GCCAAGATAT AGAAGAGGGA AATCACCAGG TGGAAGATGG ATGTAGAGAA 
ATGGCGTGTG AAGAGTTTAA TTTTGGAGAA ATATTAATGA CCCAAGTAAT CCATTCCATC 
GAGTACTGTC TGGGATGCAT CTCCAACACC GCCTCCTACC TGAGGCTCTG GGCGCTTAGC 
CTGGCTCACG CACAGTTGTC TGATGTCCTG TGGGCCATGC TGATGCGCGT GGGCCTCCGC 
GTTGACACCA CCTATGGCGT CTTGCTACTG CTCCCGGTTA TCGCGCTCTT TGCAGTTTTG 
ACCATTTTCA TCCTTCTGAT CATGGAAGGG CTTTCTGCGT TTCTTCACGC CATACGCCTC 
CACTGGGTAG AATTTCAGAA CAAATTCTAC GTTGGTGCAG GCACCAAATT TGTTCCTTTC 
TCATTCAGTC TACTTTCATC AAAGTTCAAT AACGACGACA GTGTGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V0A2 ( NM_012463.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036595.2 Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), mRNA.

Price & Availability↑

CloneID	OHu22501
Clone ID Related Accession (Same CDS sequence)	NM_012463.4 , NM_012463.3
Accession Version	NM_012463.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Homo sapiens(human)
Product	V-type proton ATPase 116 kDa subunit a isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC068531.1, DA737084.1, BI562012.1, AC117503.8 and R51339.1. This sequence is a reference standard in the RefSeqGene project. On Jul 24, 2009 this sequence version replaced NM_012463.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC068531.1, SRR1803617.270118.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTCCC TGTTCCGGAG CGAGACCATG TGCCTGGCGC AGCTCTTCCT GCAGTCGGGC 
ACGGCCTACG AGTGCCTCAG CGCCCTGGGC GAGAAAGGCC TGGTCCAGTT CCGAGACCTC 
AACCAGAACG TAAGTTCTTT CCAAAGAAAA TTTGTTGGTG AGGTGAAGAG GTGTGAAGAG 
CTAGAGCGAA TATTGGTGTA TTTGGTACAG GAAATTAATA GAGCTGATAT TCCCCTTCCT 
GAAGGAGAGG CCAGCCCTCC TGCGCCACCC CTGAAACAGG TTCTAGAAAT GCAGGAGCAG 
TTGCAGAAGC TCGAGGTTGA ACTGAGAGAA GTCACTAAGA ACAAGGAGAA ACTGAGGAAA 
AACTTGCTGG AACTGATAGA GTACACTCAC ATGCTGAGAG TGACAAAGAC CTTTGTGAAA 
CGCAATGTTG AGTTTGAACC CACTTATGAA GAATTCCCTT CCTTAGAGAG TGATTCTTTG 
TTGGATTACA GCTGTATGCA GAGGCTGGGA GCAAAACTGG GATTTGTGTC TGGCCTAATT 
AACCAAGGAA AAGTGGAAGC ATTTGAAAAA ATGTTGTGGA GAGTCTGCAA AGGGTACACC 
ATCGTGTCCT ATGCAGAACT GGATGAATCC CTTGAAGACC CTGAAACAGG GGAAGTCATA 
AAATGGTATG TCTTTTTAAT ATCCTTTTGG GGAGAGCAGA TTGGCCACAA GGTTAAGAAG 
ATATGTGATT GCTACCACTG CCACGTGTAC CCCTATCCAA ACACAGCCGA GGAGCGGAGG 
GAGATCCAGG AGGGGCTGAA CACCCGCATC CAGGATCTCT ACACTGTACT GCACAAAACC 
GAGGACTATT TGAGGCAAGT GCTATGTAAA GCCGCCGAGT CTGTCTACAG CCGTGTGATC 
CAGGTGAAGA AAATGAAGGC CATCTATCAC ATGCTGAACA TGTGCAGCTT TGACGTGACC 
AACAAGTGCC TCATTGCTGA GGTCTGGTGT CCCGAGGCGG ATCTGCAGGA CCTGCGCCGG 
GCACTGGAGG AGGGCTCGAG AGAGAGTGGT GCTACAATCC CCTCATTCAT GAATATAATC 
CCCACAAAAG AAACACCCCC CACTCGGATC CGCACCAACA AATTCACCGA GGGATTTCAG 
AACATCGTGG ATGCTTATGG AGTCGGAAGC TACAGAGAAG TCAATCCAGC TCTCTTTACC 
ATCATCACCT TCCCGTTTTT ATTTGCTGTG ATGTTTGGAG ACTTCGGACA TGGCTTTGTG 
ATGTTTTTAT TTGCCCTCTT GTTGGTGTTA AATGAAAATC ATCCCAGACT AAATCAGTCA 
CAAGAGATCA TGAGGATGTT TTTTAATGGC CGGTACATCC TCCTGCTGAT GGGGCTGTTC 
TCAGTGTACA CTGGCCTCAT CTACAACGAC TGCTTTTCAA AGTCAGTCAA CCTGTTCGGC 
TCTGGGTGGA ACGTGTCGGC CATGTACAGC TCCAGCCACC CACCCGCAGA GCATAAGAAG 
ATGGTGCTTT GGAATGACAG CGTCGTTAGA CACAACAGCA TTTTGCAGCT GGATCCAAGC 
ATTCCTGGAG TGTTCCGAGG CCCTTATCCC CTTGGCATTG ATCCTATTTG GAACTTGGCC 
ACAAATCGCC TCACTTTTCT AAACTCTTTC AAAATGAAAA TGTCCGTGAT TTTAGGAATC 
ATTCATATGA CTTTTGGAGT CATTCTGGGA ATATTTAACC ACTTGCACTT CAGGAAGAAG 
TTCAACATTT ACCTGGTTTC CATCCCGGAA CTTCTCTTCA TGCTCTGTAT CTTTGGATAC 
CTTATATTTA TGATTTTCTA CAAGTGGCTG GTTTTTTCAG CAGAAACCTC CAGAGTTGCT 
CCCAGCATTC TGATTGAATT TATTAACATG TTTTTATTCC CAGCCAGTAA AACAAGTGGC 
CTTTACACAG GGCAGGAGTA TGTCCAGAGA GTGCTGCTGG TTGTCACAGC ATTGTCTGTC 
CCTGTCCTCT TCTTGGGAAA GCCACTGTTT TTGTTGTGGC TTCACAATGG GCGTAGTTGC 
TTCGGGGTGA ACCGGAGTGG CTACACACTT ATAAGGAAAG ATAGTGAGGA AGAAGTTTCA 
TTGCTGGGAA GCCAAGATAT AGAAGAGGGA AATCACCAGG TGGAAGATGG ATGTAGAGAA 
ATGGCGTGTG AAGAGTTTAA TTTTGGAGAA ATATTAATGA CCCAAGTAAT CCATTCCATC 
GAGTACTGTC TGGGATGCAT CTCCAACACC GCCTCCTACC TGAGGCTCTG GGCGCTTAGC 
CTGGCTCACG CACAGTTGTC TGATGTCCTG TGGGCCATGC TGATGCGCGT GGGCCTCCGC 
GTTGACACCA CCTATGGCGT CTTGCTACTG CTCCCGGTTA TCGCGCTCTT TGCAGTTTTG 
ACCATTTTCA TCCTTCTGAT CATGGAAGGG CTTTCTGCGT TTCTTCACGC CATACGCCTC 
CACTGGGTAG AATTTCAGAA CAAATTCTAC GTTGGTGCAG GCACCAAATT TGTTCCTTTC 
TCATTCAGTC TACTTTCATC AAAGTTCAAT AACGACGACA GTGTGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036595.2
CDS	249..2819
Translation	MGSLFRSETMCLAQLFLQSGTAYECLSALGEKGLVQFRDLNQNVSSFQRKFVGEVKRCEELERILVYLVQEINRADIPLPEGEASPPAPPLKQVLEMQEQLQKLEVELREVTKNKEKLRKNLLELIEYTHMLRVTKTFVKRNVEFEPTYEEFPSLESDSLLDYSCMQRLGAKLGFVSGLINQGKVEAFEKMLWRVCKGYTIVSYAELDESLEDPETGEVIKWYVFLISFWGEQIGHKVKKICDCYHCHVYPYPNTAEERREIQEGLNTRIQDLYTVLHKTEDYLRQVLCKAAESVYSRVIQVKKMKAIYHMLNMCSFDVTNKCLIAEVWCPEADLQDLRRALEEGSRESGATIPSFMNIIPTKETPPTRIRTNKFTEGFQNIVDAYGVGSYREVNPALFTIITFPFLFAVMFGDFGHGFVMFLFALLLVLNENHPRLNQSQEIMRMFFNGRYILLLMGLFSVYTGLIYNDCFSKSVNLFGSGWNVSAMYSSSHPPAEHKKMVLWNDSVVRHNSILQLDPSIPGVFRGPYPLGIDPIWNLATNRLTFLNSFKMKMSVILGIIHMTFGVILGIFNHLHFRKKFNIYLVSIPELLFMLCIFGYLIFMIFYKWLVFSAETSRVAPSILIEFINMFLFPASKTSGLYTGQEYVQRVLLVVTALSVPVLFLGKPLFLLWLHNGRSCFGVNRSGYTLIRKDSEEEVSLLGSQDIEEGNHQVEDGCREMACEEFNFGEILMTQVIHSIEYCLGCISNTASYLRLWALSLAHAQLSDVLWAMLMRVGLRVDTTYGVLLLLPVIALFAVLTIFILLIMEGLSAFLHAIRLHWVEFQNKFYVGAGTKFVPFSFSLLSSKFNNDDSVA

Target ORF information:

RefSeq Version	NM_012463.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012463.3

ORF Insert Sequence:

ATGGGGTCCC TGTTCCGGAG CGAGACCATG TGCCTGGCGC AGCTCTTCCT GCAGTCGGGC 
ACGGCCTACG AGTGCCTCAG CGCCCTGGGC GAGAAAGGCC TGGTCCAGTT CCGAGACCTC 
AACCAGAACG TAAGTTCTTT CCAAAGAAAA TTTGTTGGTG AGGTGAAGAG GTGTGAAGAG 
CTAGAGCGAA TATTGGTGTA TTTGGTACAG GAAATTAATA GAGCTGATAT TCCCCTTCCT 
GAAGGAGAGG CCAGCCCTCC TGCGCCACCC CTGAAACAGG TTCTAGAAAT GCAGGAGCAG 
TTGCAGAAGC TCGAGGTTGA ACTGAGAGAA GTCACTAAGA ACAAGGAGAA ACTGAGGAAA 
AACTTGCTGG AACTGATAGA GTACACTCAC ATGCTGAGAG TGACAAAGAC CTTTGTGAAA 
CGCAATGTTG AGTTTGAACC CACTTATGAA GAATTCCCTT CCTTAGAGAG TGATTCTTTG 
TTGGATTACA GCTGTATGCA GAGGCTGGGA GCAAAACTGG GATTTGTGTC TGGCCTAATT 
AACCAAGGAA AAGTGGAAGC ATTTGAAAAA ATGTTGTGGA GAGTCTGCAA AGGGTACACC 
ATCGTGTCCT ATGCAGAACT GGATGAATCC CTTGAAGACC CTGAAACAGG GGAAGTCATA 
AAATGGTATG TCTTTTTAAT ATCCTTTTGG GGAGAGCAGA TTGGCCACAA GGTTAAGAAG 
ATATGTGATT GCTACCACTG CCACGTGTAC CCCTATCCAA ACACAGCCGA GGAGCGGAGG 
GAGATCCAGG AGGGGCTGAA CACCCGCATC CAGGATCTCT ACACTGTACT GCACAAAACC 
GAGGACTATT TGAGGCAAGT GCTATGTAAA GCCGCCGAGT CTGTCTACAG CCGTGTGATC 
CAGGTGAAGA AAATGAAGGC CATCTATCAC ATGCTGAACA TGTGCAGCTT TGACGTGACC 
AACAAGTGCC TCATTGCTGA GGTCTGGTGT CCCGAGGCGG ATCTGCAGGA CCTGCGCCGG 
GCACTGGAGG AGGGCTCGAG AGAGAGTGGT GCTACAATCC CCTCATTCAT GAATATAATC 
CCCACAAAAG AAACACCCCC CACTCGGATC CGCACCAACA AATTCACCGA GGGATTTCAG 
AACATCGTGG ATGCTTATGG AGTCGGAAGC TACAGAGAAG TCAATCCAGC TCTCTTTACC 
ATCATCACCT TCCCGTTTTT ATTTGCTGTG ATGTTTGGAG ACTTCGGACA TGGCTTTGTG 
ATGTTTTTAT TTGCCCTCTT GTTGGTGTTA AATGAAAATC ATCCCAGACT AAATCAGTCA 
CAAGAGATCA TGAGGATGTT TTTTAATGGC CGGTACATCC TCCTGCTGAT GGGGCTGTTC 
TCAGTGTACA CTGGCCTCAT CTACAACGAC TGCTTTTCAA AGTCAGTCAA CCTGTTCGGC 
TCTGGGTGGA ACGTGTCGGC CATGTACAGC TCCAGCCACC CACCCGCAGA GCATAAGAAG 
ATGGTGCTTT GGAATGACAG CGTCGTTAGA CACAACAGCA TTTTGCAGCT GGATCCAAGC 
ATTCCTGGAG TGTTCCGAGG CCCTTATCCC CTTGGCATTG ATCCTATTTG GAACTTGGCC 
ACAAATCGCC TCACTTTTCT AAACTCTTTC AAAATGAAAA TGTCCGTGAT TTTAGGAATC 
ATTCATATGA CTTTTGGAGT CATTCTGGGA ATATTTAACC ACTTGCACTT CAGGAAGAAG 
TTCAACATTT ACCTGGTTTC CATCCCGGAA CTTCTCTTCA TGCTCTGTAT CTTTGGATAC 
CTTATATTTA TGATTTTCTA CAAGTGGCTG GTTTTTTCAG CAGAAACCTC CAGAGTTGCT 
CCCAGCATTC TGATTGAATT TATTAACATG TTTTTATTCC CAGCCAGTAA AACAAGTGGC 
CTTTACACAG GGCAGGAGTA TGTCCAGAGA GTGCTGCTGG TTGTCACAGC ATTGTCTGTC 
CCTGTCCTCT TCTTGGGAAA GCCACTGTTT TTGTTGTGGC TTCACAATGG GCGTAGTTGC 
TTCGGGGTGA ACCGGAGTGG CTACACACTT ATAAGGAAAG ATAGTGAGGA AGAAGTTTCA 
TTGCTGGGAA GCCAAGATAT AGAAGAGGGA AATCACCAGG TGGAAGATGG ATGTAGAGAA 
ATGGCGTGTG AAGAGTTTAA TTTTGGAGAA ATATTAATGA CCCAAGTAAT CCATTCCATC 
GAGTACTGTC TGGGATGCAT CTCCAACACC GCCTCCTACC TGAGGCTCTG GGCGCTTAGC 
CTGGCTCACG CACAGTTGTC TGATGTCCTG TGGGCCATGC TGATGCGCGT GGGCCTCCGC 
GTTGACACCA CCTATGGCGT CTTGCTACTG CTCCCGGTTA TCGCGCTCTT TGCAGTTTTG 
ACCATTTTCA TCCTTCTGAT CATGGAAGGG CTTTCTGCGT TTCTTCACGC CATACGCCTC 
CACTGGGTAG AATTTCAGAA CAAATTCTAC GTTGGTGCAG GCACCAAATT TGTTCCTTTC 
TCATTCAGTC TACTTTCATC AAAGTTCAAT AACGACGACA GTGTGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V0A2 ( XM_024448910.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304678.1 Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu46448
Clone ID Related Accession (Same CDS sequence)	XM_024448910.1
Accession Version	XM_024448910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	V-type proton ATPase 116 kDa subunit a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCCC TGTTCCGGAG CGAGACCATG TGCCTGGCGC AGCTCTTCCT GCAGTCGGGC 
ACGGCCTACG AGTGCCTCAG CGCCCTGGGC GAGAAAGGCC TGGTCCAGTT CCGAGACCTC 
AACCAGAACG TAAGTTCTTT CCAAAGAAAA TTTGTTGGTG AGGTGAAGAG GTGTGAAGAG 
CTAGAGCGAA TATTGGTGTA TTTGGTACAG GAAATTAATA GAGCTGATAT TCCCCTTCCT 
GAAGGAGAGG CCAGCCCTCC TGCGCCACCC CTGAAACAGG TTCTAGAAAT GCAGGAGCAG 
TTGCAGAAGC TCGAGGTTGA ACTGAGAGAA GTCACTAAGA ACAAGGAGAA ACTGAGGAAA 
AACTTGCTGG AACTGATAGA GTACACTCAC ATGCTGAGAG TGACAAAGAC CTTTGTGAAA 
CGCAATGTTG AGTTTGAACC CACTTATGAA GAATTCCCTT CCTTAGAGAG TGATTCTTTG 
TTGGATTACA GCTGTATGCA GAGGCTGGGA GCAAAACTGG GATTTGTGTC TGGCCTAATT 
AACCAAGGAA AAGTGGAAGC ATTTGAAAAA ATGTTGTGGA GAGTCTGCAA AGGGTACACC 
ATCGTGTCCT ATGCAGAACT GGATGAATCC CTTGAAGACC CTGAAACAGG GGAAGTCATA 
AAATGGTATG TCTTTTTAAT ATCCTTTTGG GGAGAGCAGA TTGGCCACAA GGTTAAGAAG 
ATATGTGATT GCTACCACTG CCACGTGTAC CCCTATCCAA ACACAGCCGA GGAGCGGAGG 
GAGATCCAGG AGGGGCTGAA CACCCGCATC CAGGATCTCT ACACTGTACT GCACAAAACC 
GAGGACTATT TGAGGCAAGT GCTATGTAAA GCCGCCGAGT CTGTCTACAG CCGTGTGATC 
CAGGTGAAGA AAATGAAGGC CATCTATCAC ATGCTGAACA TGTGCAGCTT TGACGTGACC 
AACAAGTGCC TCATTGCTGA GGTCTGGTGT CCCGAGGCGG ATCTGCAGGA CCTGCGCCGG 
GCACTGGAGG AGGGCTCGAG AGAGAGTGGT GCTACAATCC CCTCATTCAT GAATATAATC 
CCCACAAAAG AAACACCCCC CACTCGGATC CGCACCAACA AATTCACCGA GGGATTTCAG 
AACATCGTGG ATGCTTATGG AGTCGGAAGC TACAGAGAAG TCAATCCAGC TCTCTTTACC 
ATCATCACCT TCCCGTTTTT ATTTGCTGTG ATGTTTGGAG ACTTCGGACA TGGCTTTGTG 
ATGTTTTTAT TTGCCCTCTT GTTGGTGTTA AATGAAAATC ATCCCAGACT AAATCAGTCA 
CAAGAGATCA TGAGGATGTT TTTTAATGGC CGGTACATCC TCCTGCTGAT GGGGCTGTTC 
TCAGTGTACA CTGGCCTCAT CTACAACGAC TGCTTTTCAA AGTCAGTCAA CCTGTTCGGC 
TCTGGGTGGA ACGTGTCGGC CATGTACAGC TCCAGCCACC CACCCGCAGA GCATAAGAAG 
ATGGTGCTTT GGAATGACAG CGTCGTTAGA CACAACAGCA TTTTGCAGCT GGATCCAAGC 
ATTCCTGGAG TGTTCCGAGG CCCTTATCCC CTTGGCATTG ATCCTATTTG GAACTTGGCC 
ACAAATCGCC TCACTTTTCT AAACTCTTTC AAAATGAAAA TGTCCGTGAT TTTAGGAATC 
ATTCATATGA CTTTTGGAGT CATTCTGGGA ATATTTAACC ACTTGCACTT CAGGAAGAAG 
TTCAACATTT ACCTGGTTTC CATCCCGGAA CTTCTCTTCA TGCTCTGTAT CTTTGGATAC 
CTTATATTTA TGATTTTCTA CAAGTGGCTG GTTTTTTCAG CAGAAACCTC CAGAGTTGCT 
CCCAGCATTC TGATTGAATT TATTAACATG TTTTTATTCC CAGCCAGTAA AACAAGTGGC 
CTTTACACAG GGCAGAGTGG CTACACACTT ATAAGGAAAG ATAGTGAGGA AGAAGTTTCA 
TTGCTGGGAA GCCAAGATAT AGAAGAGGGA AATCACCAGG TGGAAGATGG ATGTAGAGAA 
ATGGCGTGTG AAGAGTTTAA TTTTGGAGAA ATATTAATGA CCCAAGTAAT CCATTCCATC 
GAGTACTGTC TGGGATGCAT CTCCAACACC GCCTCCTACC TGAGGCTCTG GGCGCTTAGC 
CTGGCTCACG CACAGTTGTC TGATGTCCTG TGGGCCATGC TGATGCGCGT GGGCCTCCGC 
GTTGACACCA CCTATGGCGT CTTGCTACTG CTCCCGGTTA TCGCGCTCTT TGCAGTTTTG 
ACCATTTTCA TCCTTCTGAT CATGGAAGGG CTTTCTGCGT TTCTTCACGC CATACGCCTC 
CACTGGGTAG AATTTCAGAA CAAATTCTAC GTTGGTGCAG GCACCAAATT TGTTCCTTTC 
TCATTCAGTC TACTTTCATC AAAGTTCAAT AACGACGACA GTGTGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304678.1
CDS	164..2614
Translation	MGSLFRSETMCLAQLFLQSGTAYECLSALGEKGLVQFRDLNQNVSSFQRKFVGEVKRCEELERILVYLVQEINRADIPLPEGEASPPAPPLKQVLEMQEQLQKLEVELREVTKNKEKLRKNLLELIEYTHMLRVTKTFVKRNVEFEPTYEEFPSLESDSLLDYSCMQRLGAKLGFVSGLINQGKVEAFEKMLWRVCKGYTIVSYAELDESLEDPETGEVIKWYVFLISFWGEQIGHKVKKICDCYHCHVYPYPNTAEERREIQEGLNTRIQDLYTVLHKTEDYLRQVLCKAAESVYSRVIQVKKMKAIYHMLNMCSFDVTNKCLIAEVWCPEADLQDLRRALEEGSRESGATIPSFMNIIPTKETPPTRIRTNKFTEGFQNIVDAYGVGSYREVNPALFTIITFPFLFAVMFGDFGHGFVMFLFALLLVLNENHPRLNQSQEIMRMFFNGRYILLLMGLFSVYTGLIYNDCFSKSVNLFGSGWNVSAMYSSSHPPAEHKKMVLWNDSVVRHNSILQLDPSIPGVFRGPYPLGIDPIWNLATNRLTFLNSFKMKMSVILGIIHMTFGVILGIFNHLHFRKKFNIYLVSIPELLFMLCIFGYLIFMIFYKWLVFSAETSRVAPSILIEFINMFLFPASKTSGLYTGQSGYTLIRKDSEEEVSLLGSQDIEEGNHQVEDGCREMACEEFNFGEILMTQVIHSIEYCLGCISNTASYLRLWALSLAHAQLSDVLWAMLMRVGLRVDTTYGVLLLLPVIALFAVLTIFILLIMEGLSAFLHAIRLHWVEFQNKFYVGAGTKFVPFSFSLLSSKFNNDDSVA

Target ORF information:

RefSeq Version	XM_024448910.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448910.1

ORF Insert Sequence:

ATGGGGTCCC TGTTCCGGAG CGAGACCATG TGCCTGGCGC AGCTCTTCCT GCAGTCGGGC 
ACGGCCTACG AGTGCCTCAG CGCCCTGGGC GAGAAAGGCC TGGTCCAGTT CCGAGACCTC 
AACCAGAACG TAAGTTCTTT CCAAAGAAAA TTTGTTGGTG AGGTGAAGAG GTGTGAAGAG 
CTAGAGCGAA TATTGGTGTA TTTGGTACAG GAAATTAATA GAGCTGATAT TCCCCTTCCT 
GAAGGAGAGG CCAGCCCTCC TGCGCCACCC CTGAAACAGG TTCTAGAAAT GCAGGAGCAG 
TTGCAGAAGC TCGAGGTTGA ACTGAGAGAA GTCACTAAGA ACAAGGAGAA ACTGAGGAAA 
AACTTGCTGG AACTGATAGA GTACACTCAC ATGCTGAGAG TGACAAAGAC CTTTGTGAAA 
CGCAATGTTG AGTTTGAACC CACTTATGAA GAATTCCCTT CCTTAGAGAG TGATTCTTTG 
TTGGATTACA GCTGTATGCA GAGGCTGGGA GCAAAACTGG GATTTGTGTC TGGCCTAATT 
AACCAAGGAA AAGTGGAAGC ATTTGAAAAA ATGTTGTGGA GAGTCTGCAA AGGGTACACC 
ATCGTGTCCT ATGCAGAACT GGATGAATCC CTTGAAGACC CTGAAACAGG GGAAGTCATA 
AAATGGTATG TCTTTTTAAT ATCCTTTTGG GGAGAGCAGA TTGGCCACAA GGTTAAGAAG 
ATATGTGATT GCTACCACTG CCACGTGTAC CCCTATCCAA ACACAGCCGA GGAGCGGAGG 
GAGATCCAGG AGGGGCTGAA CACCCGCATC CAGGATCTCT ACACTGTACT GCACAAAACC 
GAGGACTATT TGAGGCAAGT GCTATGTAAA GCCGCCGAGT CTGTCTACAG CCGTGTGATC 
CAGGTGAAGA AAATGAAGGC CATCTATCAC ATGCTGAACA TGTGCAGCTT TGACGTGACC 
AACAAGTGCC TCATTGCTGA GGTCTGGTGT CCCGAGGCGG ATCTGCAGGA CCTGCGCCGG 
GCACTGGAGG AGGGCTCGAG AGAGAGTGGT GCTACAATCC CCTCATTCAT GAATATAATC 
CCCACAAAAG AAACACCCCC CACTCGGATC CGCACCAACA AATTCACCGA GGGATTTCAG 
AACATCGTGG ATGCTTATGG AGTCGGAAGC TACAGAGAAG TCAATCCAGC TCTCTTTACC 
ATCATCACCT TCCCGTTTTT ATTTGCTGTG ATGTTTGGAG ACTTCGGACA TGGCTTTGTG 
ATGTTTTTAT TTGCCCTCTT GTTGGTGTTA AATGAAAATC ATCCCAGACT AAATCAGTCA 
CAAGAGATCA TGAGGATGTT TTTTAATGGC CGGTACATCC TCCTGCTGAT GGGGCTGTTC 
TCAGTGTACA CTGGCCTCAT CTACAACGAC TGCTTTTCAA AGTCAGTCAA CCTGTTCGGC 
TCTGGGTGGA ACGTGTCGGC CATGTACAGC TCCAGCCACC CACCCGCAGA GCATAAGAAG 
ATGGTGCTTT GGAATGACAG CGTCGTTAGA CACAACAGCA TTTTGCAGCT GGATCCAAGC 
ATTCCTGGAG TGTTCCGAGG CCCTTATCCC CTTGGCATTG ATCCTATTTG GAACTTGGCC 
ACAAATCGCC TCACTTTTCT AAACTCTTTC AAAATGAAAA TGTCCGTGAT TTTAGGAATC 
ATTCATATGA CTTTTGGAGT CATTCTGGGA ATATTTAACC ACTTGCACTT CAGGAAGAAG 
TTCAACATTT ACCTGGTTTC CATCCCGGAA CTTCTCTTCA TGCTCTGTAT CTTTGGATAC 
CTTATATTTA TGATTTTCTA CAAGTGGCTG GTTTTTTCAG CAGAAACCTC CAGAGTTGCT 
CCCAGCATTC TGATTGAATT TATTAACATG TTTTTATTCC CAGCCAGTAA AACAAGTGGC 
CTTTACACAG GGCAGAGTGG CTACACACTT ATAAGGAAAG ATAGTGAGGA AGAAGTTTCA 
TTGCTGGGAA GCCAAGATAT AGAAGAGGGA AATCACCAGG TGGAAGATGG ATGTAGAGAA 
ATGGCGTGTG AAGAGTTTAA TTTTGGAGAA ATATTAATGA CCCAAGTAAT CCATTCCATC 
GAGTACTGTC TGGGATGCAT CTCCAACACC GCCTCCTACC TGAGGCTCTG GGCGCTTAGC 
CTGGCTCACG CACAGTTGTC TGATGTCCTG TGGGCCATGC TGATGCGCGT GGGCCTCCGC 
GTTGACACCA CCTATGGCGT CTTGCTACTG CTCCCGGTTA TCGCGCTCTT TGCAGTTTTG 
ACCATTTTCA TCCTTCTGAT CATGGAAGGG CTTTCTGCGT TTCTTCACGC CATACGCCTC 
CACTGGGTAG AATTTCAGAA CAAATTCTAC GTTGGTGCAG GCACCAAATT TGTTCCTTTC 
TCATTCAGTC TACTTTCATC AAAGTTCAAT AACGACGACA GTGTGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V0A2 ( XM_024448911.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304679.1 Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu46449
Clone ID Related Accession (Same CDS sequence)	XM_024448911.1
Accession Version	XM_024448911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	V-type proton ATPase 116 kDa subunit a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAT TTGTGTCTGG CCTAATTAAC CAAGGAAAAG TGGAAGCATT TGAAAAAATG 
TTGTGGAGAG TCTGCAAAGG GTACACCATC GTGTCCTATG CAGAACTGGA TGAATCCCTT 
GAAGACCCTG AAACAGGGGA AGTCATAAAA TGGTATGTCT TTTTAATATC CTTTTGGGGA 
GAGCAGATTG GCCACAAGGT TAAGAAGATA TGTGATTGCT ACCACTGCCA CGTGTACCCC 
TATCCAAACA CAGCCGAGGA GCGGAGGGAG ATCCAGGAGG GGCTGAACAC CCGCATCCAG 
GATCTCTACA CTGTACTGCA CAAAACCGAG GACTATTTGA GGCAAGTGCT ATGTAAAGCC 
GCCGAGTCTG TCTACAGCCG TGTGATCCAG GTGAAGAAAA TGAAGGCCAT CTATCACATG 
CTGAACATGT GCAGCTTTGA CGTGACCAAC AAGTGCCTCA TTGCTGAGGT CTGGTGTCCC 
GAGGCGGATC TGCAGGACCT GCGCCGGGCA CTGGAGGAGG GCTCGAGAGA GAGTGGTGCT 
ACAATCCCCT CATTCATGAA TATAATCCCC ACAAAAGAAA CACCCCCCAC TCGGATCCGC 
ACCAACAAAT TCACCGAGGG ATTTCAGAAC ATCGTGGATG CTTATGGAGT CGGAAGCTAC 
AGAGAAGTCA ATCCAGCTCT CTTTACCATC ATCACCTTCC CGTTTTTATT TGCTGTGATG 
TTTGGAGACT TCGGACATGG CTTTGTGATG TTTTTATTTG CCCTCTTGTT GGTGTTAAAT 
GAAAATCATC CCAGACTAAA TCAGTCACAA GAGATCATGA GGATGTTTTT TAATGGCCGG 
TACATCCTCC TGCTGATGGG GCTGTTCTCA GTGTACACTG GCCTCATCTA CAACGACTGC 
TTTTCAAAGT CAGTCAACCT GTTCGGCTCT GGGTGGAACG TGTCGGCCAT GTACAGCTCC 
AGCCACCCAC CCGCAGAGCA TAAGAAGATG GTGCTTTGGA ATGACAGCGT CGTTAGACAC 
AACAGCATTT TGCAGCTGGA TCCAAGCATT CCTGGAGTGT TCCGAGGCCC TTATCCCCTT 
GGCATTGATC CTATTTGGAA CTTGGCCACA AATCGCCTCA CTTTTCTAAA CTCTTTCAAA 
ATGAAAATGT CCGTGATTTT AGGAATCATT CATATGACTT TTGGAGTCAT TCTGGGAATA 
TTTAACCACT TGCACTTCAG GAAGAAGTTC AACATTTACC TGGTTTCCAT CCCGGAACTT 
CTCTTCATGC TCTGTATCTT TGGATACCTT ATATTTATGA TTTTCTACAA GTGGCTGGTT 
TTTTCAGCAG AAACCTCCAG AGTTGCTCCC AGCATTCTGA TTGAATTTAT TAACATGTTT 
TTATTCCCAG CCAGTAAAAC AAGTGGCCTT TACACAGGGC AGGAGTATGT CCAGAGAGTG 
CTGCTGGTTG TCACAGCATT GTCTGTCCCT GTCCTCTTCT TGGGAAAGCC ACTGTTTTTG 
TTGTGGCTTC ACAATGGGCG TAGTTGCTTC GGGGTGAACC GGAGTGGCTA CACACTTATA 
AGGAAAGATA GTGAGGAAGA AGTTTCATTG CTGGGAAGCC AAGATATAGA AGAGGGAAAT 
CACCAGGTGG AAGATGGATG TAGAGAAATG GCGTGTGAAG AGTTTAATTT TGGAGAAATA 
TTAATGACCC AAGTAATCCA TTCCATCGAG TACTGTCTGG GATGCATCTC CAACACCGCC 
TCCTACCTGA GGCTCTGGGC GCTTAGCCTG GCTCACGCAC AGTTGTCTGA TGTCCTGTGG 
GCCATGCTGA TGCGCGTGGG CCTCCGCGTT GACACCACCT ATGGCGTCTT GCTACTGCTC 
CCGGTTATCG CGCTCTTTGC AGTTTTGACC ATTTTCATCC TTCTGATCAT GGAAGGGCTT 
TCTGCGTTTC TTCACGCCAT ACGCCTCCAC TGGGTAGAAT TTCAGAACAA ATTCTACGTT 
GGTGCAGGCA CCAAATTTGT TCCTTTCTCA TTCAGTCTAC TTTCATCAAA GTTCAATAAC 
GACGACAGTG TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304679.1
CDS	102..2159
Translation	MLRFVSGLINQGKVEAFEKMLWRVCKGYTIVSYAELDESLEDPETGEVIKWYVFLISFWGEQIGHKVKKICDCYHCHVYPYPNTAEERREIQEGLNTRIQDLYTVLHKTEDYLRQVLCKAAESVYSRVIQVKKMKAIYHMLNMCSFDVTNKCLIAEVWCPEADLQDLRRALEEGSRESGATIPSFMNIIPTKETPPTRIRTNKFTEGFQNIVDAYGVGSYREVNPALFTIITFPFLFAVMFGDFGHGFVMFLFALLLVLNENHPRLNQSQEIMRMFFNGRYILLLMGLFSVYTGLIYNDCFSKSVNLFGSGWNVSAMYSSSHPPAEHKKMVLWNDSVVRHNSILQLDPSIPGVFRGPYPLGIDPIWNLATNRLTFLNSFKMKMSVILGIIHMTFGVILGIFNHLHFRKKFNIYLVSIPELLFMLCIFGYLIFMIFYKWLVFSAETSRVAPSILIEFINMFLFPASKTSGLYTGQEYVQRVLLVVTALSVPVLFLGKPLFLLWLHNGRSCFGVNRSGYTLIRKDSEEEVSLLGSQDIEEGNHQVEDGCREMACEEFNFGEILMTQVIHSIEYCLGCISNTASYLRLWALSLAHAQLSDVLWAMLMRVGLRVDTTYGVLLLLPVIALFAVLTIFILLIMEGLSAFLHAIRLHWVEFQNKFYVGAGTKFVPFSFSLLSSKFNNDDSVA

Target ORF information:

RefSeq Version	XM_024448911.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448911.1

ORF Insert Sequence:

ATGTTGAGAT TTGTGTCTGG CCTAATTAAC CAAGGAAAAG TGGAAGCATT TGAAAAAATG 
TTGTGGAGAG TCTGCAAAGG GTACACCATC GTGTCCTATG CAGAACTGGA TGAATCCCTT 
GAAGACCCTG AAACAGGGGA AGTCATAAAA TGGTATGTCT TTTTAATATC CTTTTGGGGA 
GAGCAGATTG GCCACAAGGT TAAGAAGATA TGTGATTGCT ACCACTGCCA CGTGTACCCC 
TATCCAAACA CAGCCGAGGA GCGGAGGGAG ATCCAGGAGG GGCTGAACAC CCGCATCCAG 
GATCTCTACA CTGTACTGCA CAAAACCGAG GACTATTTGA GGCAAGTGCT ATGTAAAGCC 
GCCGAGTCTG TCTACAGCCG TGTGATCCAG GTGAAGAAAA TGAAGGCCAT CTATCACATG 
CTGAACATGT GCAGCTTTGA CGTGACCAAC AAGTGCCTCA TTGCTGAGGT CTGGTGTCCC 
GAGGCGGATC TGCAGGACCT GCGCCGGGCA CTGGAGGAGG GCTCGAGAGA GAGTGGTGCT 
ACAATCCCCT CATTCATGAA TATAATCCCC ACAAAAGAAA CACCCCCCAC TCGGATCCGC 
ACCAACAAAT TCACCGAGGG ATTTCAGAAC ATCGTGGATG CTTATGGAGT CGGAAGCTAC 
AGAGAAGTCA ATCCAGCTCT CTTTACCATC ATCACCTTCC CGTTTTTATT TGCTGTGATG 
TTTGGAGACT TCGGACATGG CTTTGTGATG TTTTTATTTG CCCTCTTGTT GGTGTTAAAT 
GAAAATCATC CCAGACTAAA TCAGTCACAA GAGATCATGA GGATGTTTTT TAATGGCCGG 
TACATCCTCC TGCTGATGGG GCTGTTCTCA GTGTACACTG GCCTCATCTA CAACGACTGC 
TTTTCAAAGT CAGTCAACCT GTTCGGCTCT GGGTGGAACG TGTCGGCCAT GTACAGCTCC 
AGCCACCCAC CCGCAGAGCA TAAGAAGATG GTGCTTTGGA ATGACAGCGT CGTTAGACAC 
AACAGCATTT TGCAGCTGGA TCCAAGCATT CCTGGAGTGT TCCGAGGCCC TTATCCCCTT 
GGCATTGATC CTATTTGGAA CTTGGCCACA AATCGCCTCA CTTTTCTAAA CTCTTTCAAA 
ATGAAAATGT CCGTGATTTT AGGAATCATT CATATGACTT TTGGAGTCAT TCTGGGAATA 
TTTAACCACT TGCACTTCAG GAAGAAGTTC AACATTTACC TGGTTTCCAT CCCGGAACTT 
CTCTTCATGC TCTGTATCTT TGGATACCTT ATATTTATGA TTTTCTACAA GTGGCTGGTT 
TTTTCAGCAG AAACCTCCAG AGTTGCTCCC AGCATTCTGA TTGAATTTAT TAACATGTTT 
TTATTCCCAG CCAGTAAAAC AAGTGGCCTT TACACAGGGC AGGAGTATGT CCAGAGAGTG 
CTGCTGGTTG TCACAGCATT GTCTGTCCCT GTCCTCTTCT TGGGAAAGCC ACTGTTTTTG 
TTGTGGCTTC ACAATGGGCG TAGTTGCTTC GGGGTGAACC GGAGTGGCTA CACACTTATA 
AGGAAAGATA GTGAGGAAGA AGTTTCATTG CTGGGAAGCC AAGATATAGA AGAGGGAAAT 
CACCAGGTGG AAGATGGATG TAGAGAAATG GCGTGTGAAG AGTTTAATTT TGGAGAAATA 
TTAATGACCC AAGTAATCCA TTCCATCGAG TACTGTCTGG GATGCATCTC CAACACCGCC 
TCCTACCTGA GGCTCTGGGC GCTTAGCCTG GCTCACGCAC AGTTGTCTGA TGTCCTGTGG 
GCCATGCTGA TGCGCGTGGG CCTCCGCGTT GACACCACCT ATGGCGTCTT GCTACTGCTC 
CCGGTTATCG CGCTCTTTGC AGTTTTGACC ATTTTCATCC TTCTGATCAT GGAAGGGCTT 
TCTGCGTTTC TTCACGCCAT ACGCCTCCAC TGGGTAGAAT TTCAGAACAA ATTCTACGTT 
GGTGCAGGCA CCAAATTTGT TCCTTTCTCA TTCAGTCTAC TTTCATCAAA GTTCAATAAC 
GACGACAGTG TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V0A2 ( XM_024448912.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304680.1 Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), transcript variant X3, mRNA.

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CloneID	OHu46450
Clone ID Related Accession (Same CDS sequence)	XM_024448912.1
Accession Version	XM_024448912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	V-type proton ATPase 116 kDa subunit a isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTACTGC ACAAAACCGA GGACTATTTG AGGCAAGTGC TATGTAAAGC CGCCGAGTCT 
GTCTACAGCC GTGTGATCCA GGTGAAGAAA ATGAAGGCCA TCTATCACAT GCTGAACATG 
TGCAGCTTTG ACGTGACCAA CAAGTGCCTC ATTGCTGAGG TCTGGTGTCC CGAGGCGGAT 
CTGCAGGACC TGCGCCGGGC ACTGGAGGAG GGCTCGAGAG AGAGTGGTGC TACAATCCCC 
TCATTCATGA ATATAATCCC CACAAAAGAA ACACCCCCCA CTCGGATCCG CACCAACAAA 
TTCACCGAGG GATTTCAGAA CATCGTGGAT GCTTATGGAG TCGGAAGCTA CAGAGAAGTC 
AATCCAGCTC TCTTTACCAT CATCACCTTC CCGTTTTTAT TTGCTGTGAT GTTTGGAGAC 
TTCGGACATG GCTTTGTGAT GTTTTTATTT GCCCTCTTGT TGGTGTTAAA TGAAAATCAT 
CCCAGACTAA ATCAGTCACA AGAGATCATG AGGATGTTTT TTAATGGCCG GTACATCCTC 
CTGCTGATGG GGCTGTTCTC AGTGTACACT GGCCTCATCT ACAACGACTG CTTTTCAAAG 
TCAGTCAACC TGTTCGGCTC TGGGTGGAAC GTGTCGGCCA TGTACAGCTC CAGCCACCCA 
CCCGCAGAGC ATAAGAAGAT GGTGCTTTGG AATGACAGCG TCGTTAGACA CAACAGCATT 
TTGCAGCTGG ATCCAAGCAT TCCTGGAGTG TTCCGAGGCC CTTATCCCCT TGGCATTGAT 
CCTATTTGGA ACTTGGCCAC AAATCGCCTC ACTTTTCTAA ACTCTTTCAA AATGAAAATG 
TCCGTGATTT TAGGAATCAT TCATATGACT TTTGGAGTCA TTCTGGGAAT ATTTAACCAC 
TTGCACTTCA GGAAGAAGTT CAACATTTAC CTGGTTTCCA TCCCGGAACT TCTCTTCATG 
CTCTGTATCT TTGGATACCT TATATTTATG ATTTTCTACA AGTGGCTGGT TTTTTCAGCA 
GAAACCTCCA GAGTTGCTCC CAGCATTCTG ATTGAATTTA TTAACATGTT TTTATTCCCA 
GCCAGTAAAA CAAGTGGCCT TTACACAGGG CAGGAGTATG TCCAGAGAGT GCTGCTGGTT 
GTCACAGCAT TGTCTGTCCC TGTCCTCTTC TTGGGAAAGC CACTGTTTTT GTTGTGGCTT 
CACAATGGGC GTAGTTGCTT CGGGGTGAAC CGGAGTGGCT ACACACTTAT AAGGAAAGAT 
AGTGAGGAAG AAGTTTCATT GCTGGGAAGC CAAGATATAG AAGAGGGAAA TCACCAGGTG 
GAAGATGGAT GTAGAGAAAT GGCGTGTGAA GAGTTTAATT TTGGAGAAAT ATTAATGACC 
CAAGTAATCC ATTCCATCGA GTACTGTCTG GGATGCATCT CCAACACCGC CTCCTACCTG 
AGGCTCTGGG CGCTTAGCCT GGCTCACGCA CAGTTGTCTG ATGTCCTGTG GGCCATGCTG 
ATGCGCGTGG GCCTCCGCGT TGACACCACC TATGGCGTCT TGCTACTGCT CCCGGTTATC 
GCGCTCTTTG CAGTTTTGAC CATTTTCATC CTTCTGATCA TGGAAGGGCT TTCTGCGTTT 
CTTCACGCCA TACGCCTCCA CTGGGTAGAA TTTCAGAACA AATTCTACGT TGGTGCAGGC 
ACCAAATTTG TTCCTTTCTC ATTCAGTCTA CTTTCATCAA AGTTCAATAA CGACGACAGT 
GTGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304680.1
CDS	119..1867
Translation	MVLHKTEDYLRQVLCKAAESVYSRVIQVKKMKAIYHMLNMCSFDVTNKCLIAEVWCPEADLQDLRRALEEGSRESGATIPSFMNIIPTKETPPTRIRTNKFTEGFQNIVDAYGVGSYREVNPALFTIITFPFLFAVMFGDFGHGFVMFLFALLLVLNENHPRLNQSQEIMRMFFNGRYILLLMGLFSVYTGLIYNDCFSKSVNLFGSGWNVSAMYSSSHPPAEHKKMVLWNDSVVRHNSILQLDPSIPGVFRGPYPLGIDPIWNLATNRLTFLNSFKMKMSVILGIIHMTFGVILGIFNHLHFRKKFNIYLVSIPELLFMLCIFGYLIFMIFYKWLVFSAETSRVAPSILIEFINMFLFPASKTSGLYTGQEYVQRVLLVVTALSVPVLFLGKPLFLLWLHNGRSCFGVNRSGYTLIRKDSEEEVSLLGSQDIEEGNHQVEDGCREMACEEFNFGEILMTQVIHSIEYCLGCISNTASYLRLWALSLAHAQLSDVLWAMLMRVGLRVDTTYGVLLLLPVIALFAVLTIFILLIMEGLSAFLHAIRLHWVEFQNKFYVGAGTKFVPFSFSLLSSKFNNDDSVA

Target ORF information:

RefSeq Version	XM_024448912.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448912.1

ORF Insert Sequence:

ATGGTACTGC ACAAAACCGA GGACTATTTG AGGCAAGTGC TATGTAAAGC CGCCGAGTCT 
GTCTACAGCC GTGTGATCCA GGTGAAGAAA ATGAAGGCCA TCTATCACAT GCTGAACATG 
TGCAGCTTTG ACGTGACCAA CAAGTGCCTC ATTGCTGAGG TCTGGTGTCC CGAGGCGGAT 
CTGCAGGACC TGCGCCGGGC ACTGGAGGAG GGCTCGAGAG AGAGTGGTGC TACAATCCCC 
TCATTCATGA ATATAATCCC CACAAAAGAA ACACCCCCCA CTCGGATCCG CACCAACAAA 
TTCACCGAGG GATTTCAGAA CATCGTGGAT GCTTATGGAG TCGGAAGCTA CAGAGAAGTC 
AATCCAGCTC TCTTTACCAT CATCACCTTC CCGTTTTTAT TTGCTGTGAT GTTTGGAGAC 
TTCGGACATG GCTTTGTGAT GTTTTTATTT GCCCTCTTGT TGGTGTTAAA TGAAAATCAT 
CCCAGACTAA ATCAGTCACA AGAGATCATG AGGATGTTTT TTAATGGCCG GTACATCCTC 
CTGCTGATGG GGCTGTTCTC AGTGTACACT GGCCTCATCT ACAACGACTG CTTTTCAAAG 
TCAGTCAACC TGTTCGGCTC TGGGTGGAAC GTGTCGGCCA TGTACAGCTC CAGCCACCCA 
CCCGCAGAGC ATAAGAAGAT GGTGCTTTGG AATGACAGCG TCGTTAGACA CAACAGCATT 
TTGCAGCTGG ATCCAAGCAT TCCTGGAGTG TTCCGAGGCC CTTATCCCCT TGGCATTGAT 
CCTATTTGGA ACTTGGCCAC AAATCGCCTC ACTTTTCTAA ACTCTTTCAA AATGAAAATG 
TCCGTGATTT TAGGAATCAT TCATATGACT TTTGGAGTCA TTCTGGGAAT ATTTAACCAC 
TTGCACTTCA GGAAGAAGTT CAACATTTAC CTGGTTTCCA TCCCGGAACT TCTCTTCATG 
CTCTGTATCT TTGGATACCT TATATTTATG ATTTTCTACA AGTGGCTGGT TTTTTCAGCA 
GAAACCTCCA GAGTTGCTCC CAGCATTCTG ATTGAATTTA TTAACATGTT TTTATTCCCA 
GCCAGTAAAA CAAGTGGCCT TTACACAGGG CAGGAGTATG TCCAGAGAGT GCTGCTGGTT 
GTCACAGCAT TGTCTGTCCC TGTCCTCTTC TTGGGAAAGC CACTGTTTTT GTTGTGGCTT 
CACAATGGGC GTAGTTGCTT CGGGGTGAAC CGGAGTGGCT ACACACTTAT AAGGAAAGAT 
AGTGAGGAAG AAGTTTCATT GCTGGGAAGC CAAGATATAG AAGAGGGAAA TCACCAGGTG 
GAAGATGGAT GTAGAGAAAT GGCGTGTGAA GAGTTTAATT TTGGAGAAAT ATTAATGACC 
CAAGTAATCC ATTCCATCGA GTACTGTCTG GGATGCATCT CCAACACCGC CTCCTACCTG 
AGGCTCTGGG CGCTTAGCCT GGCTCACGCA CAGTTGTCTG ATGTCCTGTG GGCCATGCTG 
ATGCGCGTGG GCCTCCGCGT TGACACCACC TATGGCGTCT TGCTACTGCT CCCGGTTATC 
GCGCTCTTTG CAGTTTTGAC CATTTTCATC CTTCTGATCA TGGAAGGGCT TTCTGCGTTT 
CTTCACGCCA TACGCCTCCA CTGGGTAGAA TTTCAGAACA AATTCTACGT TGGTGCAGGC 
ACCAAATTTG TTCCTTTCTC ATTCAGTCTA CTTTCATCAA AGTTCAATAA CGACGACAGT 
GTGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular mechanisms of cutis laxa and distal renal tubular acidosis-causing mutations in V-ATPase
The Journal of biological chemistry(2018 Jan)
Esmail S,Kartner N,Yao Y,Kim JW,Reithmeier RAF,Manolson MF

The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels.
eLife6(2017 03)
Miles AL,Burr SP,Grice GL,Nathan JA

Cancer derived peptide of vacuolar ATPase 'a2' isoform promotes neutrophil migration by autocrine secretion of IL-8.
Scientific reports636865(2016 Nov)
Ibrahim SA,Kulshrestha A,Katara GK,Amin MA,Beaman KD

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ATP6V0A2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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