GPC4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPC4
Entrez Gene ID	2239
Full Name	glypican 4
Synonyms	K-glypican
General protein information	Preferred Names glypican 4 Names glypican-4 dJ900E8.1 (glypican 4) glypican proteoglycan 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq26.2
Summary	Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. [provided by RefSeq, Jul 2008].
Disorder MIM:	300168

mRNA and Protein(s)

mRNA	Protein	Name
NM_001448.2	NP_001439.2	glypican-4 precursor
NM_001448.3	NP_001439.2	glypican-4 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gpc4	NP_001014130.1
Bos taurus (cattle)	GPC4	NP_001192713.1
Mus musculus (house mouse)	Gpc4	NP_032176.2
Gallus gallus (chicken)	GPC4	XP_420222.3
Danio rerio (zebrafish)	gpc4	NP_571935.2
Xenopus tropicalis (tropical clawed frog)	gpc4	XP_002932634.1
Canis lupus familiaris (dog)	GPC4	XP_549265.2
	GPC4	XP_001097252.1
Drosophila melanogaster (fruit fly)	dlp	NP_996075.2
Caenorhabditis elegans (roundworm)	gpn-1	NP_510582.1
Homo sapiens (human)	GPC4	NP_001439.2
Pan troglodytes (chimpanzee)	GPC4	XP_529161.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003559	XP_313306.5

Gene Ontology
Bibliography

Related articles in PubMed

Expression of Glypican-4 in the brains of epileptic patients and epileptic animals and its effects on epileptic seizures.
Xiong Y, Zhang Y, Zheng F, Yang Y, Xu X, Wang W, Zhu B, Wang X
Biochemical and biophysical research communications478(1)241-246(2016 09)

Glypican-4 gene polymorphism (rs1048369) and susceptibility to Epstein-Barr virus-associated and -negative gastric carcinoma.
Zhao D, Liu S, Sun L, Zhao Z, Liu S, Kuang X, Shu J, Luo B
Virus research22052-6(2016 07)

Association of serum glypican-4 levels with cardiovascular risk predictors in women with polycystic ovary syndrome - a pilot study.
J?drzejuk D, Lwow F, Kuliczkowska-P?aksej J, Hirnle L, Trzmiel-Bira A, Lenarcik-Kabza A, Kolackov K, ?aczma?ski ?, Milewicz A
Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology32(3)223-6(2016)

Wnt signaling in midbrain dopaminergic neuron development and regenerative medicine for Parkinson's disease.
Arenas E
Journal of molecular cell biology6(1)42-53(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

According to behavioral studies, downregulation of Gpc4 by Gpc4 siRNA decreased spontaneous seizure frequency, while upregulation of Gpc4 by recombinant Gpc4 overexpression led to a converse result. These findings support the hypothesis that increased expression of Gpc4 in the brain is associated with epileptic seizures.
Title: Expression of Glypican-4 in the brains of epileptic patients and epileptic animals and its effects on epileptic seizures.

The T allele of GPC4 may represent a risk factor for Epstein-Barr virus-associated gastric carcinoma.
Title: Glypican-4 gene polymorphism (rs1048369) and susceptibility to Epstein-Barr virus-associated and -negative gastric carcinoma.

serum level elevated in polycystic ovary syndrome and correlated with fat distribution and cardiovascular risk
Title: Association of serum glypican-4 levels with cardiovascular risk predictors in women with polycystic ovary syndrome - a pilot study.

Data suggest gender difference in circulating GPC4 levels in nonalcoholic fatty liver disease; GPC4 levels in women appear to correlate with cardiometabolic risk factors (adiposity/body fat distribution, insulin resistance, and arterial stiffness).
Title: Association of glypican-4 with body fat distribution, insulin resistance, and nonalcoholic fatty liver disease.

Findings establish that Gpc4 acts at the interface of extrinsic and intrinsic signal regulation to fine tune stem cell fate.
Title: Modulating Glypican4 suppresses tumorigenicity of embryonic stem cells while preserving self-renewal and pluripotency.

The following GPC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18892	NM_001448.3 Latest version!	Homo sapiens glypican 4 (GPC4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
View Old Accession Versions >>
OHu18892	NM_001448.2	Homo sapiens glypican 4 (GPC4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPC4 ( NM_001448.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001439.2 Homo sapiens glypican 4 (GPC4), mRNA.

Price & Availability↑

CloneID	OHu18892
Clone ID Related Accession (Same CDS sequence)	NM_001448.2 , NM_001448.3
Accession Version	NM_001448.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	glypican-4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF030186.1. This sequence is a reference standard in the RefSeqGene project. On Jun 26, 2002 this sequence version replaced NM_001448.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF030186.1, SRR1660809.223928.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACGGT TCGGCTTGCC CGCGCTTCTC TGCACCCTGG CAGTGCTCAG CGCCGCGCTG 
CTGGCTGCCG AGCTCAAGTC GAAAAGTTGC TCGGAAGTGC GACGTCTTTA CGTGTCCAAA 
GGCTTCAACA AGAACGATGC CCCCCTCCAC GAGATCAACG GTGATCATTT GAAGATCTGT 
CCCCAGGGTT CTACCTGCTG CTCTCAAGAG ATGGAGGAGA AGTACAGCCT GCAAAGTAAA 
GATGATTTCA AAAGTGTGGT CAGCGAACAG TGCAATCATT TGCAAGCTGT CTTTGCTTCA 
CGTTACAAGA AGTTTGATGA ATTCTTCAAA GAACTACTTG AAAATGCAGA GAAATCCCTG 
AATGATATGT TTGTGAAGAC ATATGGCCAT TTATACATGC AAAATTCTGA GCTATTTAAA 
GATCTCTTCG TAGAGTTGAA ACGTTACTAC GTGGTGGGAA ATGTGAACCT GGAAGAAATG 
CTAAATGACT TCTGGGCTCG CCTCCTGGAG CGGATGTTCC GCCTGGTGAA CTCCCAGTAC 
CACTTTACAG ATGAGTATCT GGAATGTGTG AGCAAGTATA CGGAGCAGCT GAAGCCCTTC 
GGAGATGTCC CTCGCAAATT GAAGCTCCAG GTTACTCGTG CTTTTGTAGC AGCCCGTACT 
TTCGCTCAAG GCTTAGCGGT TGCGGGAGAT GTCGTGAGCA AGGTCTCCGT GGTAAACCCC 
ACAGCCCAGT GTACCCATGC CCTGTTGAAG ATGATCTACT GCTCCCACTG CCGGGGTCTC 
GTGACTGTGA AGCCATGTTA CAACTACTGC TCAAACATCA TGAGAGGCTG TTTGGCCAAC 
CAAGGGGATC TCGATTTTGA ATGGAACAAT TTCATAGATG CTATGCTGAT GGTGGCAGAG 
AGGCTAGAGG GTCCTTTCAA CATTGAATCG GTCATGGATC CCATCGATGT GAAGATTTCT 
GATGCTATTA TGAACATGCA GGATAATAGT GTTCAAGTGT CTCAGAAGGT TTTCCAGGGA 
TGTGGACCCC CCAAGCCCCT CCCAGCTGGA CGAATTTCTC GTTCCATCTC TGAAAGTGCC 
TTCAGTGCTC GCTTCAGACC ACATCACCCC GAGGAACGCC CAACCACAGC AGCTGGCACT 
AGTTTGGACC GACTGGTTAC TGATGTCAAG GAGAAACTGA AACAGGCCAA GAAATTCTGG 
TCCTCCCTTC CGAGCAACGT TTGCAACGAT GAGAGGATGG CTGCAGGAAA CGGCAATGAG 
GATGACTGTT GGAATGGGAA AGGCAAAAGC AGGTACCTGT TTGCAGTGAC AGGAAATGGA 
TTAGCCAACC AGGGCAACAA CCCAGAGGTC CAGGTTGACA CCAGCAAACC AGACATACTG 
ATCCTTCGTC AAATCATGGC TCTTCGAGTG ATGACCAGCA AGATGAAGAA TGCATACAAT 
GGGAACGACG TGGACTTCTT TGATATCAGT GATGAAAGTA GTGGAGAAGG AAGTGGAAGT 
GGCTGTGAGT ATCAGCAGTG CCCTTCAGAG TTTGACTACA ATGCCACTGA CCATGCTGGG 
AAGAGTGCCA ATGAGAAAGC CGACAGTGCT GGTGTCCGTC CTGGGGCACA GGCCTACCTC 
CTCACTGTCT TCTGCATCTT GTTCCTGGTT ATGCAGAGAG AGTGGAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001439.2
CDS	213..1883
Translation	MARFGLPALLCTLAVLSAALLAAELKSKSCSEVRRLYVSKGFNKNDAPLHEINGDHLKICPQGSTCCSQEMEEKYSLQSKDDFKSVVSEQCNHLQAVFASRYKKFDEFFKELLENAEKSLNDMFVKTYGHLYMQNSELFKDLFVELKRYYVVGNVNLEEMLNDFWARLLERMFRLVNSQYHFTDEYLECVSKYTEQLKPFGDVPRKLKLQVTRAFVAARTFAQGLAVAGDVVSKVSVVNPTAQCTHALLKMIYCSHCRGLVTVKPCYNYCSNIMRGCLANQGDLDFEWNNFIDAMLMVAERLEGPFNIESVMDPIDVKISDAIMNMQDNSVQVSQKVFQGCGPPKPLPAGRISRSISESAFSARFRPHHPEERPTTAAGTSLDRLVTDVKEKLKQAKKFWSSLPSNVCNDERMAAGNGNEDDCWNGKGKSRYLFAVTGNGLANQGNNPEVQVDTSKPDILILRQIMALRVMTSKMKNAYNGNDVDFFDISDESSGEGSGSGCEYQQCPSEFDYNATDHAGKSANEKADSAGVRPGAQAYLLTVFCILFLVMQREWR

Target ORF information:

RefSeq Version	NM_001448.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glypican 4 (GPC4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001448.2

ORF Insert Sequence:

ATGGCACGGT TCGGCTTGCC CGCGCTTCTC TGCACCCTGG CAGTGCTCAG CGCCGCGCTG 
CTGGCTGCCG AGCTCAAGTC GAAAAGTTGC TCGGAAGTGC GACGTCTTTA CGTGTCCAAA 
GGCTTCAACA AGAACGATGC CCCCCTCCAC GAGATCAACG GTGATCATTT GAAGATCTGT 
CCCCAGGGTT CTACCTGCTG CTCTCAAGAG ATGGAGGAGA AGTACAGCCT GCAAAGTAAA 
GATGATTTCA AAAGTGTGGT CAGCGAACAG TGCAATCATT TGCAAGCTGT CTTTGCTTCA 
CGTTACAAGA AGTTTGATGA ATTCTTCAAA GAACTACTTG AAAATGCAGA GAAATCCCTG 
AATGATATGT TTGTGAAGAC ATATGGCCAT TTATACATGC AAAATTCTGA GCTATTTAAA 
GATCTCTTCG TAGAGTTGAA ACGTTACTAC GTGGTGGGAA ATGTGAACCT GGAAGAAATG 
CTAAATGACT TCTGGGCTCG CCTCCTGGAG CGGATGTTCC GCCTGGTGAA CTCCCAGTAC 
CACTTTACAG ATGAGTATCT GGAATGTGTG AGCAAGTATA CGGAGCAGCT GAAGCCCTTC 
GGAGATGTCC CTCGCAAATT GAAGCTCCAG GTTACTCGTG CTTTTGTAGC AGCCCGTACT 
TTCGCTCAAG GCTTAGCGGT TGCGGGAGAT GTCGTGAGCA AGGTCTCCGT GGTAAACCCC 
ACAGCCCAGT GTACCCATGC CCTGTTGAAG ATGATCTACT GCTCCCACTG CCGGGGTCTC 
GTGACTGTGA AGCCATGTTA CAACTACTGC TCAAACATCA TGAGAGGCTG TTTGGCCAAC 
CAAGGGGATC TCGATTTTGA ATGGAACAAT TTCATAGATG CTATGCTGAT GGTGGCAGAG 
AGGCTAGAGG GTCCTTTCAA CATTGAATCG GTCATGGATC CCATCGATGT GAAGATTTCT 
GATGCTATTA TGAACATGCA GGATAATAGT GTTCAAGTGT CTCAGAAGGT TTTCCAGGGA 
TGTGGACCCC CCAAGCCCCT CCCAGCTGGA CGAATTTCTC GTTCCATCTC TGAAAGTGCC 
TTCAGTGCTC GCTTCAGACC ACATCACCCC GAGGAACGCC CAACCACAGC AGCTGGCACT 
AGTTTGGACC GACTGGTTAC TGATGTCAAG GAGAAACTGA AACAGGCCAA GAAATTCTGG 
TCCTCCCTTC CGAGCAACGT TTGCAACGAT GAGAGGATGG CTGCAGGAAA CGGCAATGAG 
GATGACTGTT GGAATGGGAA AGGCAAAAGC AGGTACCTGT TTGCAGTGAC AGGAAATGGA 
TTAGCCAACC AGGGCAACAA CCCAGAGGTC CAGGTTGACA CCAGCAAACC AGACATACTG 
ATCCTTCGTC AAATCATGGC TCTTCGAGTG ATGACCAGCA AGATGAAGAA TGCATACAAT 
GGGAACGACG TGGACTTCTT TGATATCAGT GATGAAAGTA GTGGAGAAGG AAGTGGAAGT 
GGCTGTGAGT ATCAGCAGTG CCCTTCAGAG TTTGACTACA ATGCCACTGA CCATGCTGGG 
AAGAGTGCCA ATGAGAAAGC CGACAGTGCT GGTGTCCGTC CTGGGGCACA GGCCTACCTC 
CTCACTGTCT TCTGCATCTT GTTCCTGGTT ATGCAGAGAG AGTGGAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPC4 ( NM_001448.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001439.2 Homo sapiens glypican 4 (GPC4), mRNA.

Price & Availability↑

CloneID	OHu18892
Clone ID Related Accession (Same CDS sequence)	NM_001448.2 , NM_001448.3
Accession Version	NM_001448.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	glypican-4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL109623.9 and AL034400.2. This sequence is a reference standard in the RefSeqGene project. On Nov 26, 2018 this sequence version replaced NM_001448.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.223928.1, SRR1660809.149973.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000370828.4/ ENSP00000359864.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCACGGT TCGGCTTGCC CGCGCTTCTC TGCACCCTGG CAGTGCTCAG CGCCGCGCTG 
CTGGCTGCCG AGCTCAAGTC GAAAAGTTGC TCGGAAGTGC GACGTCTTTA CGTGTCCAAA 
GGCTTCAACA AGAACGATGC CCCCCTCCAC GAGATCAACG GTGATCATTT GAAGATCTGT 
CCCCAGGGTT CTACCTGCTG CTCTCAAGAG ATGGAGGAGA AGTACAGCCT GCAAAGTAAA 
GATGATTTCA AAAGTGTGGT CAGCGAACAG TGCAATCATT TGCAAGCTGT CTTTGCTTCA 
CGTTACAAGA AGTTTGATGA ATTCTTCAAA GAACTACTTG AAAATGCAGA GAAATCCCTG 
AATGATATGT TTGTGAAGAC ATATGGCCAT TTATACATGC AAAATTCTGA GCTATTTAAA 
GATCTCTTCG TAGAGTTGAA ACGTTACTAC GTGGTGGGAA ATGTGAACCT GGAAGAAATG 
CTAAATGACT TCTGGGCTCG CCTCCTGGAG CGGATGTTCC GCCTGGTGAA CTCCCAGTAC 
CACTTTACAG ATGAGTATCT GGAATGTGTG AGCAAGTATA CGGAGCAGCT GAAGCCCTTC 
GGAGATGTCC CTCGCAAATT GAAGCTCCAG GTTACTCGTG CTTTTGTAGC AGCCCGTACT 
TTCGCTCAAG GCTTAGCGGT TGCGGGAGAT GTCGTGAGCA AGGTCTCCGT GGTAAACCCC 
ACAGCCCAGT GTACCCATGC CCTGTTGAAG ATGATCTACT GCTCCCACTG CCGGGGTCTC 
GTGACTGTGA AGCCATGTTA CAACTACTGC TCAAACATCA TGAGAGGCTG TTTGGCCAAC 
CAAGGGGATC TCGATTTTGA ATGGAACAAT TTCATAGATG CTATGCTGAT GGTGGCAGAG 
AGGCTAGAGG GTCCTTTCAA CATTGAATCG GTCATGGATC CCATCGATGT GAAGATTTCT 
GATGCTATTA TGAACATGCA GGATAATAGT GTTCAAGTGT CTCAGAAGGT TTTCCAGGGA 
TGTGGACCCC CCAAGCCCCT CCCAGCTGGA CGAATTTCTC GTTCCATCTC TGAAAGTGCC 
TTCAGTGCTC GCTTCAGACC ACATCACCCC GAGGAACGCC CAACCACAGC AGCTGGCACT 
AGTTTGGACC GACTGGTTAC TGATGTCAAG GAGAAACTGA AACAGGCCAA GAAATTCTGG 
TCCTCCCTTC CGAGCAACGT TTGCAACGAT GAGAGGATGG CTGCAGGAAA CGGCAATGAG 
GATGACTGTT GGAATGGGAA AGGCAAAAGC AGGTACCTGT TTGCAGTGAC AGGAAATGGA 
TTAGCCAACC AGGGCAACAA CCCAGAGGTC CAGGTTGACA CCAGCAAACC AGACATACTG 
ATCCTTCGTC AAATCATGGC TCTTCGAGTG ATGACCAGCA AGATGAAGAA TGCATACAAT 
GGGAACGACG TGGACTTCTT TGATATCAGT GATGAAAGTA GTGGAGAAGG AAGTGGAAGT 
GGCTGTGAGT ATCAGCAGTG CCCTTCAGAG TTTGACTACA ATGCCACTGA CCATGCTGGG 
AAGAGTGCCA ATGAGAAAGC CGACAGTGCT GGTGTCCGTC CTGGGGCACA GGCCTACCTC 
CTCACTGTCT TCTGCATCTT GTTCCTGGTT ATGCAGAGAG AGTGGAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001439.2
CDS	525..2195
Translation	MARFGLPALLCTLAVLSAALLAAELKSKSCSEVRRLYVSKGFNKNDAPLHEINGDHLKICPQGSTCCSQEMEEKYSLQSKDDFKSVVSEQCNHLQAVFASRYKKFDEFFKELLENAEKSLNDMFVKTYGHLYMQNSELFKDLFVELKRYYVVGNVNLEEMLNDFWARLLERMFRLVNSQYHFTDEYLECVSKYTEQLKPFGDVPRKLKLQVTRAFVAARTFAQGLAVAGDVVSKVSVVNPTAQCTHALLKMIYCSHCRGLVTVKPCYNYCSNIMRGCLANQGDLDFEWNNFIDAMLMVAERLEGPFNIESVMDPIDVKISDAIMNMQDNSVQVSQKVFQGCGPPKPLPAGRISRSISESAFSARFRPHHPEERPTTAAGTSLDRLVTDVKEKLKQAKKFWSSLPSNVCNDERMAAGNGNEDDCWNGKGKSRYLFAVTGNGLANQGNNPEVQVDTSKPDILILRQIMALRVMTSKMKNAYNGNDVDFFDISDESSGEGSGSGCEYQQCPSEFDYNATDHAGKSANEKADSAGVRPGAQAYLLTVFCILFLVMQREWR

Target ORF information:

RefSeq Version	NM_001448.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glypican 4 (GPC4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001448.3

ORF Insert Sequence:

ATGGCACGGT TCGGCTTGCC CGCGCTTCTC TGCACCCTGG CAGTGCTCAG CGCCGCGCTG 
CTGGCTGCCG AGCTCAAGTC GAAAAGTTGC TCGGAAGTGC GACGTCTTTA CGTGTCCAAA 
GGCTTCAACA AGAACGATGC CCCCCTCCAC GAGATCAACG GTGATCATTT GAAGATCTGT 
CCCCAGGGTT CTACCTGCTG CTCTCAAGAG ATGGAGGAGA AGTACAGCCT GCAAAGTAAA 
GATGATTTCA AAAGTGTGGT CAGCGAACAG TGCAATCATT TGCAAGCTGT CTTTGCTTCA 
CGTTACAAGA AGTTTGATGA ATTCTTCAAA GAACTACTTG AAAATGCAGA GAAATCCCTG 
AATGATATGT TTGTGAAGAC ATATGGCCAT TTATACATGC AAAATTCTGA GCTATTTAAA 
GATCTCTTCG TAGAGTTGAA ACGTTACTAC GTGGTGGGAA ATGTGAACCT GGAAGAAATG 
CTAAATGACT TCTGGGCTCG CCTCCTGGAG CGGATGTTCC GCCTGGTGAA CTCCCAGTAC 
CACTTTACAG ATGAGTATCT GGAATGTGTG AGCAAGTATA CGGAGCAGCT GAAGCCCTTC 
GGAGATGTCC CTCGCAAATT GAAGCTCCAG GTTACTCGTG CTTTTGTAGC AGCCCGTACT 
TTCGCTCAAG GCTTAGCGGT TGCGGGAGAT GTCGTGAGCA AGGTCTCCGT GGTAAACCCC 
ACAGCCCAGT GTACCCATGC CCTGTTGAAG ATGATCTACT GCTCCCACTG CCGGGGTCTC 
GTGACTGTGA AGCCATGTTA CAACTACTGC TCAAACATCA TGAGAGGCTG TTTGGCCAAC 
CAAGGGGATC TCGATTTTGA ATGGAACAAT TTCATAGATG CTATGCTGAT GGTGGCAGAG 
AGGCTAGAGG GTCCTTTCAA CATTGAATCG GTCATGGATC CCATCGATGT GAAGATTTCT 
GATGCTATTA TGAACATGCA GGATAATAGT GTTCAAGTGT CTCAGAAGGT TTTCCAGGGA 
TGTGGACCCC CCAAGCCCCT CCCAGCTGGA CGAATTTCTC GTTCCATCTC TGAAAGTGCC 
TTCAGTGCTC GCTTCAGACC ACATCACCCC GAGGAACGCC CAACCACAGC AGCTGGCACT 
AGTTTGGACC GACTGGTTAC TGATGTCAAG GAGAAACTGA AACAGGCCAA GAAATTCTGG 
TCCTCCCTTC CGAGCAACGT TTGCAACGAT GAGAGGATGG CTGCAGGAAA CGGCAATGAG 
GATGACTGTT GGAATGGGAA AGGCAAAAGC AGGTACCTGT TTGCAGTGAC AGGAAATGGA 
TTAGCCAACC AGGGCAACAA CCCAGAGGTC CAGGTTGACA CCAGCAAACC AGACATACTG 
ATCCTTCGTC AAATCATGGC TCTTCGAGTG ATGACCAGCA AGATGAAGAA TGCATACAAT 
GGGAACGACG TGGACTTCTT TGATATCAGT GATGAAAGTA GTGGAGAAGG AAGTGGAAGT 
GGCTGTGAGT ATCAGCAGTG CCCTTCAGAG TTTGACTACA ATGCCACTGA CCATGCTGGG 
AAGAGTGCCA ATGAGAAAGC CGACAGTGCT GGTGTCCGTC CTGGGGCACA GGCCTACCTC 
CTCACTGTCT TCTGCATCTT GTTCCTGGTT ATGCAGAGAG AGTGGAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of Glypican-4 in the brains of epileptic patients and epileptic animals and its effects on epileptic seizures.
Biochemical and biophysical research communications478(1)241-246(2016 09)
Xiong Y,Zhang Y,Zheng F,Yang Y,Xu X,Wang W,Zhu B,Wang X

Glypican-4 gene polymorphism (rs1048369) and susceptibility to Epstein-Barr virus-associated and -negative gastric carcinoma.
Virus research22052-6(2016 07)
Zhao D,Liu S,Sun L,Zhao Z,Liu S,Kuang X,Shu J,Luo B

Association of serum glypican-4 levels with cardiovascular risk predictors in women with polycystic ovary syndrome - a pilot study.
Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology32(3)223-6(2016)
J?drzejuk D,Lwow F,Kuliczkowska-P?aksej J,Hirnle L,Trzmiel-Bira A,Lenarcik-Kabza A,Kolackov K,?aczma?ski ?,Milewicz A

Wnt signaling in midbrain dopaminergic neuron development and regenerative medicine for Parkinson's disease.
Journal of molecular cell biology6(1)42-53(2014 Feb)
Arenas E