DDX53 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX53
Entrez Gene ID	168400
Full Name	DEAD-box helicase 53
Synonyms	CAGE,CT26
General protein information	Preferred Names DEAD-box helicase 53 Names DEAD box protein 53 DEAD (Asp-Glu-Ala-Asp) box polypeptide 53 DEAD-box protein CAGE cancer-associated gene protein cancer/testis antigen 26 probable ATP-dependent RNA helicase DDX53
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp22.11
Summary	This intronless gene encodes a protein which contains several domains found in members of the DEAD-box helicase protein family. Other members of this protein family participate in ATP-dependent RNA unwinding. [provided by RefSeq, Sep 2011].

mRNA and Protein(s)

mRNA	Protein	Name
NM_182699.3	NP_874358.2	DEAD box protein 53
NM_182699.4	NP_874358.2	DEAD box protein 53

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDX53	NP_874358.2
Pan troglodytes (chimpanzee)	DDX53	XP_003317435.1
Canis lupus familiaris (dog)	DDX53	XP_005642533.1
	DDX53	XP_001087519.1

Gene Ontology
Bibliography

Related articles in PubMed

DDX53 Regulates Cancer Stem Cell-Like Properties by Binding to SOX-2.
Kim Y, Yeon M, Jeoung D
Molecules and cells40(5)322-330(2017 May)

DDX53 Promotes Cancer Stem Cell-Like Properties and Autophagy.
Kim H, Kim Y, Jeoung D
Molecules and cells40(1)54-65(2017 Jan)

miR-30a Regulates the Expression of CAGE and p53 and Regulates the Response to Anti-Cancer Drugs.
Park D, Kim H, Kim Y, Jeoung D
Molecules and cells39(4)299-309(2016 Apr)

Histone Deacetylase-3/CAGE Axis Targets EGFR Signaling and Regulates the Response to Anti-Cancer Drugs.
Kim H, Kim Y, Goh H, Jeoung D
Molecules and cells39(3)229-41(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DDX53 promotes stem cell-like properties, autophagy, and confers resistance to anti-cancer drugs in breast cancer cells.
Title: DDX53 Promotes Cancer Stem Cell-Like Properties and Autophagy.

Results provide evidence that miR-30a forms a positive feedback loop with CAGE to regulate the expression of p53 confering resistance to anti-cancer drugs.
Title: miR-30a Regulates the Expression of CAGE and p53 and Regulates the Response to Anti-Cancer Drugs.

Results show the direct regulation of CAGE expression by HDAC3 and that HDAC3-CAGE axis regulates the activation of EGFR. HDAC3 targets CAGE to regulate the tumorigenic potential and angiogenic potential of cancer cells.
Title: Histone Deacetylase-3/CAGE Axis Targets EGFR Signaling and Regulates the Response to Anti-Cancer Drugs.

miR-217-CAGE feedback loop serves as a target for overcoming resistance to various anti-cancer drugs, including EGFR and HER2 inhibitors.
Title: miR-217 and CAGE form feedback loop and regulates the response to anti-cancer drugs through EGFR and HER2.

CAGE induced the interaction between histone deacetylase 2 (HDAC2) and Snail, which exerted a negative effect on p53 expression in neoplastic cells; CAGE confers drug resistance by regulating expression of p53 through HDAC2.
Title: Cancer/testis antigen CAGE exerts negative regulation on p53 expression through HDAC2 and confers resistance to anti-cancer drugs.

The following DDX53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18550	NM_182699.4 Latest version!	Homo sapiens DEAD-box helicase 53 (DDX53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
View Old Accession Versions >>
OHu18550	NM_182699.3	Homo sapiens DEAD-box helicase 53 (DDX53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX53 ( NM_182699.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_874358.2 Homo sapiens DEAD-box helicase 53 (DDX53), mRNA.

Price & Availability↑

CloneID	OHu18550
Clone ID Related Accession (Same CDS sequence)	NM_182699.3 , NM_182699.4
Accession Version	NM_182699.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	DEAD box protein 53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY039237.1 and BC067878.1. This sequence is a reference standard in the RefSeqGene project. On Sep 23, 2011 this sequence version replaced NM_182699.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: AY039237.1, BC067878.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCACT GGGCCCCAGA GTGGAAGAGG GCGGAGGCTA ATCCAAGAGA CCTTGGGGCC 
AGCTGGGATG TCAGGGGCAG CAGAGGCAGT GGCTGGAGTG GCCCCTTCGG CCATCAGGGA 
CCGAGAGCAG CAGGCTCCCG TGAACCACCA CTCTGCTTTA AAATAAAGAA CAATATGGTT 
GGTGTGGTCA TTGGTTACAG TGGATCAAAA ATAAAAGATC TACAACATTC GACAAACACT 
AAAATACAGA TCATAAACGG GGAATCTGAA GCAAAAGTCA GAATTTTTGG CAATAGGGAA 
ATGAAAGCAA AGGCCAAAGC GGCTATAGAA ACACTTATTA GAAAACAAGA AAGCTACAAC 
TCAGAATCCA GTGTGGATAA TGCTGCATCC CAAACCCCTA TTGGAAGAAA TCTAGGCAGA 
AATGACATTG TTGGAGAAGC TGAGCCATTG TCAAATTGGG ATCGCATTAG GGCAGCAGTC 
GTGGAGTGCG AAAAGAGAAA ATGGGCAGAT CTACCACCAG TTAAGAAAAA CTTTTACATA 
GAATCCAAAG CAACAAGCTG CATGTCTGAA ATGCAGGTGA TTAACTGGAG AAAGGAAAAT 
TTCAACATAA CGTGTGATGA CTTGAAAAGT GGTGAAAAGC GTCTCATTCC AAAACCAACT 
TGCAGGTTTA AAGACGCTTT TCAGCAATAC CCTGATCTTC TGAAAAGCAT AATAAGGGTA 
GGGATTGTAA AGCCAACGCC AATTCAGTCA CAGGCATGGC CAATTATTCT ACAAGGAATA 
GATCTTATAG TAGTTGCACA AACCGGAACA GGGAAAACAT TGTCCTATCT AATGCCTGGG 
TTTATTCATC TTGATTCTCA ACCAATATCT AGAGAGCAAA GGAATGGGCC TGGGATGCTA 
GTCCTTACAC CCACTAGAGA GTTGGCTCTT CACGTGGAAG CTGAATGTTC AAAGTATTCA 
TATAAAGGTC TCAAAAGCAT TTGCATATAT GGTGGTAGAA ACAGAAATGG ACAAATAGAA 
GACATTAGCA AAGGTGTAGA TATCATTATT GCAACTCCTG GGAGGCTGAA TGACCTACAA 
ATGAATAACT CTGTCAACCT AAGAAGCATA ACCTACTTGG TTATAGATGA GGCAGATAAA 
ATGCTGGATA TGGAATTTGA ACCCCAGATA AGGAAGATTT TATTAGATGT GCGCCCAGAC 
CGACAGACTG TTATGACAAG TGCAACTTGG CCAGATACTG TACGTCAACT AGCACTTTCT 
TATTTGAAAG ATCCTATGAT TGTTTATGTT GGTAATCTGA ATCTAGTGGC TGTAAATACA 
GTGAAGCAAA ATATAATTGT TACCACAGAA AAAGAAAAAC GAGCTCTCAC CCAAGAATTC 
GTAGAGAACA TGTCACCCAA CGACAAAGTC ATCATGTTTG TCAGCCAAAA ACATATTGCT 
GATGACTTGT CAAGCGACTT CAATATCCAA GGCATATCTG CAGAATCATT ACATGGCAAC 
AGTGAACAGA GTGATCAAGA GCGAGCAGTA GAGGACTTTA AAAGCGGAAA CATAAAGATA 
CTGATTACAA CTGATATAGT ATCCCGAGGT CTTGATCTTA ATGATGTCAC ACATGTATAT 
AATTATGATT TCCCAAGGAA TATTGACGTA TATGTACACA GAGTAGGGTA CATTGGACGG 
ACAGGAAAGA CTGGCACATC AGTTACCCTC ATCACTCAGA GAGATTCGAA AATGGCCGGT 
GAATTGATTA AAATTCTGGA CAGAGCAAAT CAGAGTGTTC CGGAAGATCT TGTAGTAATG 
GCTGAGCAAT ACAAGTTAAA TCAACAAAAG AGGCACAGAG AAACACGATC AAGAAAACCT 
GGACAAAGAC GCAAGGAGTT TTATTTTTTA AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_874358.2
CDS	98..1993
Translation	MSHWAPEWKRAEANPRDLGASWDVRGSRGSGWSGPFGHQGPRAAGSREPPLCFKIKNNMVGVVIGYSGSKIKDLQHSTNTKIQIINGESEAKVRIFGNREMKAKAKAAIETLIRKQESYNSESSVDNAASQTPIGRNLGRNDIVGEAEPLSNWDRIRAAVVECEKRKWADLPPVKKNFYIESKATSCMSEMQVINWRKENFNITCDDLKSGEKRLIPKPTCRFKDAFQQYPDLLKSIIRVGIVKPTPIQSQAWPIILQGIDLIVVAQTGTGKTLSYLMPGFIHLDSQPISREQRNGPGMLVLTPTRELALHVEAECSKYSYKGLKSICIYGGRNRNGQIEDISKGVDIIIATPGRLNDLQMNNSVNLRSITYLVIDEADKMLDMEFEPQIRKILLDVRPDRQTVMTSATWPDTVRQLALSYLKDPMIVYVGNLNLVAVNTVKQNIIVTTEKEKRALTQEFVENMSPNDKVIMFVSQKHIADDLSSDFNIQGISAESLHGNSEQSDQERAVEDFKSGNIKILITTDIVSRGLDLNDVTHVYNYDFPRNIDVYVHRVGYIGRTGKTGTSVTLITQRDSKMAGELIKILDRANQSVPEDLVVMAEQYKLNQQKRHRETRSRKPGQRRKEFYFLS

Target ORF information:

RefSeq Version	NM_182699.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 53 (DDX53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182699.3

ORF Insert Sequence:

ATGTCCCACT GGGCCCCAGA GTGGAAGAGG GCGGAGGCTA ATCCAAGAGA CCTTGGGGCC 
AGCTGGGATG TCAGGGGCAG CAGAGGCAGT GGCTGGAGTG GCCCCTTCGG CCATCAGGGA 
CCGAGAGCAG CAGGCTCCCG TGAACCACCA CTCTGCTTTA AAATAAAGAA CAATATGGTT 
GGTGTGGTCA TTGGTTACAG TGGATCAAAA ATAAAAGATC TACAACATTC GACAAACACT 
AAAATACAGA TCATAAACGG GGAATCTGAA GCAAAAGTCA GAATTTTTGG CAATAGGGAA 
ATGAAAGCAA AGGCCAAAGC GGCTATAGAA ACACTTATTA GAAAACAAGA AAGCTACAAC 
TCAGAATCCA GTGTGGATAA TGCTGCATCC CAAACCCCTA TTGGAAGAAA TCTAGGCAGA 
AATGACATTG TTGGAGAAGC TGAGCCATTG TCAAATTGGG ATCGCATTAG GGCAGCAGTC 
GTGGAGTGCG AAAAGAGAAA ATGGGCAGAT CTACCACCAG TTAAGAAAAA CTTTTACATA 
GAATCCAAAG CAACAAGCTG CATGTCTGAA ATGCAGGTGA TTAACTGGAG AAAGGAAAAT 
TTCAACATAA CGTGTGATGA CTTGAAAAGT GGTGAAAAGC GTCTCATTCC AAAACCAACT 
TGCAGGTTTA AAGACGCTTT TCAGCAATAC CCTGATCTTC TGAAAAGCAT AATAAGGGTA 
GGGATTGTAA AGCCAACGCC AATTCAGTCA CAGGCATGGC CAATTATTCT ACAAGGAATA 
GATCTTATAG TAGTTGCACA AACCGGAACA GGGAAAACAT TGTCCTATCT AATGCCTGGG 
TTTATTCATC TTGATTCTCA ACCAATATCT AGAGAGCAAA GGAATGGGCC TGGGATGCTA 
GTCCTTACAC CCACTAGAGA GTTGGCTCTT CACGTGGAAG CTGAATGTTC AAAGTATTCA 
TATAAAGGTC TCAAAAGCAT TTGCATATAT GGTGGTAGAA ACAGAAATGG ACAAATAGAA 
GACATTAGCA AAGGTGTAGA TATCATTATT GCAACTCCTG GGAGGCTGAA TGACCTACAA 
ATGAATAACT CTGTCAACCT AAGAAGCATA ACCTACTTGG TTATAGATGA GGCAGATAAA 
ATGCTGGATA TGGAATTTGA ACCCCAGATA AGGAAGATTT TATTAGATGT GCGCCCAGAC 
CGACAGACTG TTATGACAAG TGCAACTTGG CCAGATACTG TACGTCAACT AGCACTTTCT 
TATTTGAAAG ATCCTATGAT TGTTTATGTT GGTAATCTGA ATCTAGTGGC TGTAAATACA 
GTGAAGCAAA ATATAATTGT TACCACAGAA AAAGAAAAAC GAGCTCTCAC CCAAGAATTC 
GTAGAGAACA TGTCACCCAA CGACAAAGTC ATCATGTTTG TCAGCCAAAA ACATATTGCT 
GATGACTTGT CAAGCGACTT CAATATCCAA GGCATATCTG CAGAATCATT ACATGGCAAC 
AGTGAACAGA GTGATCAAGA GCGAGCAGTA GAGGACTTTA AAAGCGGAAA CATAAAGATA 
CTGATTACAA CTGATATAGT ATCCCGAGGT CTTGATCTTA ATGATGTCAC ACATGTATAT 
AATTATGATT TCCCAAGGAA TATTGACGTA TATGTACACA GAGTAGGGTA CATTGGACGG 
ACAGGAAAGA CTGGCACATC AGTTACCCTC ATCACTCAGA GAGATTCGAA AATGGCCGGT 
GAATTGATTA AAATTCTGGA CAGAGCAAAT CAGAGTGTTC CGGAAGATCT TGTAGTAATG 
GCTGAGCAAT ACAAGTTAAA TCAACAAAAG AGGCACAGAG AAACACGATC AAGAAAACCT 
GGACAAAGAC GCAAGGAGTT TTATTTTTTA AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX53 ( NM_182699.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_874358.2 Homo sapiens DEAD-box helicase 53 (DDX53), mRNA.

Price & Availability↑

CloneID	OHu18550
Clone ID Related Accession (Same CDS sequence)	NM_182699.3 , NM_182699.4
Accession Version	NM_182699.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	DEAD box protein 53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117517.7, AY039237.1 and BC067878.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_182699.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC067878.1, AY039237.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000327968.7/ ENSP00000368667.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCCACT GGGCCCCAGA GTGGAAGAGG GCGGAGGCTA ATCCAAGAGA CCTTGGGGCC 
AGCTGGGATG TCAGGGGCAG CAGAGGCAGT GGCTGGAGTG GCCCCTTCGG CCATCAGGGA 
CCGAGAGCAG CAGGCTCCCG TGAACCACCA CTCTGCTTTA AAATAAAGAA CAATATGGTT 
GGTGTGGTCA TTGGTTACAG TGGATCAAAA ATAAAAGATC TACAACATTC GACAAACACT 
AAAATACAGA TCATAAACGG GGAATCTGAA GCAAAAGTCA GAATTTTTGG CAATAGGGAA 
ATGAAAGCAA AGGCCAAAGC GGCTATAGAA ACACTTATTA GAAAACAAGA AAGCTACAAC 
TCAGAATCCA GTGTGGATAA TGCTGCATCC CAAACCCCTA TTGGAAGAAA TCTAGGCAGA 
AATGACATTG TTGGAGAAGC TGAGCCATTG TCAAATTGGG ATCGCATTAG GGCAGCAGTC 
GTGGAGTGCG AAAAGAGAAA ATGGGCAGAT CTACCACCAG TTAAGAAAAA CTTTTACATA 
GAATCCAAAG CAACAAGCTG CATGTCTGAA ATGCAGGTGA TTAACTGGAG AAAGGAAAAT 
TTCAACATAA CGTGTGATGA CTTGAAAAGT GGTGAAAAGC GTCTCATTCC AAAACCAACT 
TGCAGGTTTA AAGACGCTTT TCAGCAATAC CCTGATCTTC TGAAAAGCAT AATAAGGGTA 
GGGATTGTAA AGCCAACGCC AATTCAGTCA CAGGCATGGC CAATTATTCT ACAAGGAATA 
GATCTTATAG TAGTTGCACA AACCGGAACA GGGAAAACAT TGTCCTATCT AATGCCTGGG 
TTTATTCATC TTGATTCTCA ACCAATATCT AGAGAGCAAA GGAATGGGCC TGGGATGCTA 
GTCCTTACAC CCACTAGAGA GTTGGCTCTT CACGTGGAAG CTGAATGTTC AAAGTATTCA 
TATAAAGGTC TCAAAAGCAT TTGCATATAT GGTGGTAGAA ACAGAAATGG ACAAATAGAA 
GACATTAGCA AAGGTGTAGA TATCATTATT GCAACTCCTG GGAGGCTGAA TGACCTACAA 
ATGAATAACT CTGTCAACCT AAGAAGCATA ACCTACTTGG TTATAGATGA GGCAGATAAA 
ATGCTGGATA TGGAATTTGA ACCCCAGATA AGGAAGATTT TATTAGATGT GCGCCCAGAC 
CGACAGACTG TTATGACAAG TGCAACTTGG CCAGATACTG TACGTCAACT AGCACTTTCT 
TATTTGAAAG ATCCTATGAT TGTTTATGTT GGTAATCTGA ATCTAGTGGC TGTAAATACA 
GTGAAGCAAA ATATAATTGT TACCACAGAA AAAGAAAAAC GAGCTCTCAC CCAAGAATTC 
GTAGAGAACA TGTCACCCAA CGACAAAGTC ATCATGTTTG TCAGCCAAAA ACATATTGCT 
GATGACTTGT CAAGCGACTT CAATATCCAA GGCATATCTG CAGAATCATT ACATGGCAAC 
AGTGAACAGA GTGATCAAGA GCGAGCAGTA GAGGACTTTA AAAGCGGAAA CATAAAGATA 
CTGATTACAA CTGATATAGT ATCCCGAGGT CTTGATCTTA ATGATGTCAC ACATGTATAT 
AATTATGATT TCCCAAGGAA TATTGACGTA TATGTACACA GAGTAGGGTA CATTGGACGG 
ACAGGAAAGA CTGGCACATC AGTTACCCTC ATCACTCAGA GAGATTCGAA AATGGCCGGT 
GAATTGATTA AAATTCTGGA CAGAGCAAAT CAGAGTGTTC CGGAAGATCT TGTAGTAATG 
GCTGAGCAAT ACAAGTTAAA TCAACAAAAG AGGCACAGAG AAACACGATC AAGAAAACCT 
GGACAAAGAC GCAAGGAGTT TTATTTTTTA AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_874358.2
CDS	99..1994
Translation	MSHWAPEWKRAEANPRDLGASWDVRGSRGSGWSGPFGHQGPRAAGSREPPLCFKIKNNMVGVVIGYSGSKIKDLQHSTNTKIQIINGESEAKVRIFGNREMKAKAKAAIETLIRKQESYNSESSVDNAASQTPIGRNLGRNDIVGEAEPLSNWDRIRAAVVECEKRKWADLPPVKKNFYIESKATSCMSEMQVINWRKENFNITCDDLKSGEKRLIPKPTCRFKDAFQQYPDLLKSIIRVGIVKPTPIQSQAWPIILQGIDLIVVAQTGTGKTLSYLMPGFIHLDSQPISREQRNGPGMLVLTPTRELALHVEAECSKYSYKGLKSICIYGGRNRNGQIEDISKGVDIIIATPGRLNDLQMNNSVNLRSITYLVIDEADKMLDMEFEPQIRKILLDVRPDRQTVMTSATWPDTVRQLALSYLKDPMIVYVGNLNLVAVNTVKQNIIVTTEKEKRALTQEFVENMSPNDKVIMFVSQKHIADDLSSDFNIQGISAESLHGNSEQSDQERAVEDFKSGNIKILITTDIVSRGLDLNDVTHVYNYDFPRNIDVYVHRVGYIGRTGKTGTSVTLITQRDSKMAGELIKILDRANQSVPEDLVVMAEQYKLNQQKRHRETRSRKPGQRRKEFYFLS

Target ORF information:

RefSeq Version	NM_182699.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 53 (DDX53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_182699.4

ORF Insert Sequence:

ATGTCCCACT GGGCCCCAGA GTGGAAGAGG GCGGAGGCTA ATCCAAGAGA CCTTGGGGCC 
AGCTGGGATG TCAGGGGCAG CAGAGGCAGT GGCTGGAGTG GCCCCTTCGG CCATCAGGGA 
CCGAGAGCAG CAGGCTCCCG TGAACCACCA CTCTGCTTTA AAATAAAGAA CAATATGGTT 
GGTGTGGTCA TTGGTTACAG TGGATCAAAA ATAAAAGATC TACAACATTC GACAAACACT 
AAAATACAGA TCATAAACGG GGAATCTGAA GCAAAAGTCA GAATTTTTGG CAATAGGGAA 
ATGAAAGCAA AGGCCAAAGC GGCTATAGAA ACACTTATTA GAAAACAAGA AAGCTACAAC 
TCAGAATCCA GTGTGGATAA TGCTGCATCC CAAACCCCTA TTGGAAGAAA TCTAGGCAGA 
AATGACATTG TTGGAGAAGC TGAGCCATTG TCAAATTGGG ATCGCATTAG GGCAGCAGTC 
GTGGAGTGCG AAAAGAGAAA ATGGGCAGAT CTACCACCAG TTAAGAAAAA CTTTTACATA 
GAATCCAAAG CAACAAGCTG CATGTCTGAA ATGCAGGTGA TTAACTGGAG AAAGGAAAAT 
TTCAACATAA CGTGTGATGA CTTGAAAAGT GGTGAAAAGC GTCTCATTCC AAAACCAACT 
TGCAGGTTTA AAGACGCTTT TCAGCAATAC CCTGATCTTC TGAAAAGCAT AATAAGGGTA 
GGGATTGTAA AGCCAACGCC AATTCAGTCA CAGGCATGGC CAATTATTCT ACAAGGAATA 
GATCTTATAG TAGTTGCACA AACCGGAACA GGGAAAACAT TGTCCTATCT AATGCCTGGG 
TTTATTCATC TTGATTCTCA ACCAATATCT AGAGAGCAAA GGAATGGGCC TGGGATGCTA 
GTCCTTACAC CCACTAGAGA GTTGGCTCTT CACGTGGAAG CTGAATGTTC AAAGTATTCA 
TATAAAGGTC TCAAAAGCAT TTGCATATAT GGTGGTAGAA ACAGAAATGG ACAAATAGAA 
GACATTAGCA AAGGTGTAGA TATCATTATT GCAACTCCTG GGAGGCTGAA TGACCTACAA 
ATGAATAACT CTGTCAACCT AAGAAGCATA ACCTACTTGG TTATAGATGA GGCAGATAAA 
ATGCTGGATA TGGAATTTGA ACCCCAGATA AGGAAGATTT TATTAGATGT GCGCCCAGAC 
CGACAGACTG TTATGACAAG TGCAACTTGG CCAGATACTG TACGTCAACT AGCACTTTCT 
TATTTGAAAG ATCCTATGAT TGTTTATGTT GGTAATCTGA ATCTAGTGGC TGTAAATACA 
GTGAAGCAAA ATATAATTGT TACCACAGAA AAAGAAAAAC GAGCTCTCAC CCAAGAATTC 
GTAGAGAACA TGTCACCCAA CGACAAAGTC ATCATGTTTG TCAGCCAAAA ACATATTGCT 
GATGACTTGT CAAGCGACTT CAATATCCAA GGCATATCTG CAGAATCATT ACATGGCAAC 
AGTGAACAGA GTGATCAAGA GCGAGCAGTA GAGGACTTTA AAAGCGGAAA CATAAAGATA 
CTGATTACAA CTGATATAGT ATCCCGAGGT CTTGATCTTA ATGATGTCAC ACATGTATAT 
AATTATGATT TCCCAAGGAA TATTGACGTA TATGTACACA GAGTAGGGTA CATTGGACGG 
ACAGGAAAGA CTGGCACATC AGTTACCCTC ATCACTCAGA GAGATTCGAA AATGGCCGGT 
GAATTGATTA AAATTCTGGA CAGAGCAAAT CAGAGTGTTC CGGAAGATCT TGTAGTAATG 
GCTGAGCAAT ACAAGTTAAA TCAACAAAAG AGGCACAGAG AAACACGATC AAGAAAACCT 
GGACAAAGAC GCAAGGAGTT TTATTTTTTA AGTTGA