VCAN cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VCAN
Entrez Gene ID	1462
Full Name	versican
Synonyms	CSPG2,ERVR,GHAP,PG-M,WGN,WGN1
General protein information	Preferred Names versican Names versican core protein chondroitin sulfate proteoglycan core protein 2 glial hyaluronate-binding protein large fibroblast proteoglycan versican proteoglycan
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q14.2-q14.3
Summary	This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009].
Disorder MIM:	118661
Disorder Html:	Wagner syndrome 1, 143200 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001126336.2	NP_001119808.1	versican core protein isoform 2 precursor
NM_001164097.1	NP_001157569.1	versican core protein isoform 3 precursor
NM_001164098.1	NP_001157570.1	versican core protein isoform 4 precursor
NM_004385.5	NP_004376.2	versican core protein isoform 1 precursor
NM_004385.4	NP_004376.2	versican core protein isoform 1 precursor
NM_001126336.3	NP_001119808.1	versican core protein isoform 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	VCAN	NP_004376.2
Mus musculus (house mouse)	Vcan	NP_001074718.1
Bos taurus (cattle)	VCAN	NP_851378.1
	VCAN	XP_001112269.1
Canis lupus familiaris (dog)	VCAN	XP_003434465.1
Rattus norvegicus (Norway rat)	Vcan	NP_001164029.1
Pan troglodytes (chimpanzee)	VCAN	XP_517667.2

Gene Ontology
Bibliography

Related articles in PubMed

WAGNER syndrome: anatomic, functional and genetic characterization of a Portuguese family.
Ara?jo JR, Tavares-Ferreira J, Estrela-Silva S, Rocha P, Brand?o E, Faria PA, Falc?o-Reis F, Rocha-Sousa A
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie256(1)163-171(2018 Jan)

MiR-135a-5p modulates biological functions of thyroid carcinoma cells via targeting VCAN 3'-UTR.
Zhao X, Sun Z, Li H, Jiang F, Zhou J, Zhang L
Cancer biomarkers : section A of Disease markers20(2)207-216(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The macular dysfunction on mfERG was profound and of early onset. A heterozygous mutation in intron 7 of the VCAN gene (c.4004-1G > A) was found.
Title: WAGNER syndrome: anatomic, functional and genetic characterization of a Portuguese family.

Versican V0 and V1 isoforms were upregulated in the uterine leiomyomas of symptomatic versus asymptomatic women. Abundant cleaved versican was detected in leiomyoma and myometrium, as well as in myometrial and leiomyoma cell lines. VCAN siRNA did not effect cell proliferation, apoptosis, or smooth muscle markers, but reduced ESR1 and PR-A expression.
Title: Versican Proteolysis by ADAMTS Proteases and Its Influence on Sex Steroid Receptor Expression in Uterine Leiomyoma.

Data indicate that serum versican levels were significantly decreased in polycystic ovary syndrome (PCOS) patients, and that serum ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin motif-1) and versican levels were significantly and positively correlated with each other.
Title: Role of Versican and ADAMTS-1 in Polycystic Ovary Syndrome.

Removal or knockdown of versican may be a possible therapeutic strategy for increasing deposition of insoluble elastin and stimulating repair of elastic fibers in COPD lung.
Title: Deposition of insoluble elastin by pulmonary fibroblasts from patients with COPD is increased by treatment with versican siRNA.

Lumican and versican protein expression are associated with colorectal adenoma-to-carcinoma progression
Title: Lumican and versican protein expression are associated with colorectal adenoma-to-carcinoma progression.

The following VCAN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCAN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22613	NM_001126336.3 Latest version!	Homo sapiens versican (VCAN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu22135	NM_001164098.1 Latest version!	Homo sapiens versican (VCAN), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu22133	NM_001164097.1 Latest version!	Homo sapiens versican (VCAN), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu22418	NM_004385.5 Latest version!	Homo sapiens versican (VCAN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu22418	NM_004385.4	Homo sapiens versican (VCAN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu22613	NM_001126336.2	Homo sapiens versican (VCAN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VCAN ( NM_001126336.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001119808.1 Homo sapiens versican (VCAN), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22613
Clone ID Related Accession (Same CDS sequence)	NM_001126336.2 , NM_001126336.3
Accession Version	NM_001126336.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	versican core protein isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA361670.1, D32039.1, BC050524.1, DT217515.1 and AC026696.5. On Aug 13, 2009 this sequence version replaced NM_001126336.1. Transcript Variant: This variant (2), also known as PG-M(V3), omits two coding exons, compared to variant 1. This results in a shorter protein (isoform 2) with no chondroitin sulfate attachment domain. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.71064.1, D32039.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGTTCATAA ATATAAAGAG CATCTTATGG ATGTGTTCAA CCTTAATAGT AACCCATGCG 
CTACATAAAG TCAAAGTGGG AAAAAGCCCA CCGGTGAGGG GCTCCCTCTC TGGAAAAGTC 
AGCCTACCTT GTCATTTTTC AACGATGCCT ACTTTGCCAC CCAGTTACAA CACCAGTGAA 
TTTCTCCGCA TCAAATGGTC TAAGATTGAA GTGGACAAAA ATGGAAAAGA TTTGAAAGAG 
ACTACTGTCC TTGTGGCCCA AAATGGAAAT ATCAAGATTG GTCAGGACTA CAAAGGGAGA 
GTGTCTGTGC CCACACATCC CGAGGCTGTG GGCGATGCCT CCCTCACTGT GGTCAAGCTG 
CTGGCAAGTG ATGCGGGTCT TTACCGCTGT GACGTCATGT ACGGGATTGA AGACACACAA 
GACACGGTGT CACTGACTGT GGATGGGGTT GTGTTTCACT ACAGGGCGGC AACCAGCAGG 
TACACACTGA ATTTTGAGGC TGCTCAGAAG GCTTGTTTGG ACGTTGGGGC AGTCATAGCA 
ACTCCAGAGC AGCTCTTTGC TGCCTATGAA GATGGATTTG AGCAGTGTGA CGCAGGCTGG 
CTGGCTGATC AGACTGTCAG ATATCCCATC CGGGCTCCCA GAGTAGGCTG TTATGGAGAT 
AAGATGGGAA AGGCAGGAGT CAGGACTTAT GGATTCCGTT CTCCCCAGGA AACTTACGAT 
GTGTATTGTT ATGTGGATCA TCTGGATGGT GATGTGTTCC ACCTCACTGT CCCCAGTAAA 
TTCACCTTCG AGGAGGCTGC AAAAGAGTGT GAAAACCAGG ATGCCAGGCT GGCAACAGTG 
GGGGAACTCC AGGCGGCATG GAGGAACGGC TTTGACCAGT GCGATTACGG GTGGCTGTCG 
GATGCCAGCG TGCGCCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACAGGCT TCCCTCCCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACGACCTGAT CGCTGCAAAA TGAACCCGTG CCTTAACGGA 
GGCACCTGTT ATCCTACTGA AACTTCCTAC GTATGCACCT GTGTGCCAGG ATACAGCGGA 
GACCAGTGTG AACTTGATTT TGATGAATGT CACTCTAATC CCTGTCGTAA TGGAGCCACT 
TGTGTTGATG GTTTTAACAC ATTCAGGTGC CTCTGCCTTC CAAGTTATGT TGGTGCACTT 
TGTGAGCAAG ATACCGAGAC ATGTGACTAT GGCTGGCACA AATTCCAAGG GCAGTGCTAC 
AAATACTTTG CCCATCGACG CACATGGGAT GCAGCTGAAC GGGAATGCCG TCTGCAGGGT 
GCCCATCTCA CAAGCATCCT GTCTCACGAA GAACAAATGT TTGTTAATCG TGTGGGCCAT 
GATTATCAGT GGATAGGCCT CAATGACAAG ATGTTTGAGC ATGACTTCCG TTGGACTGAT 
GGCAGCACAC TGCAATACGA GAATTGGAGA CCCAACCAGC CAGACAGCTT CTTTTCTGCT 
GGAGAAGACT GTGTTGTAAT CATTTGGCAT GAGAATGGCC AGTGGAATGA TGTTCCCTGC 
AATTACCATC TCACCTATAC GTGCAAGAAA GGAACAGTCG CTTGCGGCCA GCCCCCTGTT 
GTAGAAAATG CCAAGACCTT TGGAAAGATG AAACCTCGTT ATGAAATCAA CTCCCTGATT 
AGATACCACT GCAAAGATGG TTTCATTCAA CGTCACCTTC CAACTATCCG GTGCTTAGGA 
AATGGAAGAT GGGCTATACC TAAAATTACC TGCATGAACC CATCTGCATA CCAAAGGACT 
TATTCTATGA AATACTTTAA AAATTCCTCA TCAGCAAAGG ACAATTCAAT AAATACATCC 
AAACATGATC ATCGTTGGAG CCGGAGGTGG CAGGAGTCGA GGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119808.1
CDS	357..2324
Translation	MFINIKSILWMCSTLIVTHALHKVKVGKSPPVRGSLSGKVSLPCHFSTMPTLPPSYNTSEFLRIKWSKIEVDKNGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEAVGDASLTVVKLLASDAGLYRCDVMYGIEDTQDTVSLTVDGVVFHYRAATSRYTLNFEAAQKACLDVGAVIATPEQLFAAYEDGFEQCDAGWLADQTVRYPIRAPRVGCYGDKMGKAGVRTYGFRSPQETYDVYCYVDHLDGDVFHLTVPSKFTFEEAAKECENQDARLATVGELQAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPPPDSRFDAYCFKRPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDQCELDFDECHSNPCRNGATCVDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAIPKITCMNPSAYQRTYSMKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Target ORF information:

RefSeq Version	NM_001126336.2
Organism	Homo sapiens(human)
Definition	Homo sapiens versican (VCAN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126336.2

ORF Insert Sequence:

ATGTTCATAA ATATAAAGAG CATCTTATGG ATGTGTTCAA CCTTAATAGT AACCCATGCG 
CTACATAAAG TCAAAGTGGG AAAAAGCCCA CCGGTGAGGG GCTCCCTCTC TGGAAAAGTC 
AGCCTACCTT GTCATTTTTC AACGATGCCT ACTTTGCCAC CCAGTTACAA CACCAGTGAA 
TTTCTCCGCA TCAAATGGTC TAAGATTGAA GTGGACAAAA ATGGAAAAGA TTTGAAAGAG 
ACTACTGTCC TTGTGGCCCA AAATGGAAAT ATCAAGATTG GTCAGGACTA CAAAGGGAGA 
GTGTCTGTGC CCACACATCC CGAGGCTGTG GGCGATGCCT CCCTCACTGT GGTCAAGCTG 
CTGGCAAGTG ATGCGGGTCT TTACCGCTGT GACGTCATGT ACGGGATTGA AGACACACAA 
GACACGGTGT CACTGACTGT GGATGGGGTT GTGTTTCACT ACAGGGCGGC AACCAGCAGG 
TACACACTGA ATTTTGAGGC TGCTCAGAAG GCTTGTTTGG ACGTTGGGGC AGTCATAGCA 
ACTCCAGAGC AGCTCTTTGC TGCCTATGAA GATGGATTTG AGCAGTGTGA CGCAGGCTGG 
CTGGCTGATC AGACTGTCAG ATATCCCATC CGGGCTCCCA GAGTAGGCTG TTATGGAGAT 
AAGATGGGAA AGGCAGGAGT CAGGACTTAT GGATTCCGTT CTCCCCAGGA AACTTACGAT 
GTGTATTGTT ATGTGGATCA TCTGGATGGT GATGTGTTCC ACCTCACTGT CCCCAGTAAA 
TTCACCTTCG AGGAGGCTGC AAAAGAGTGT GAAAACCAGG ATGCCAGGCT GGCAACAGTG 
GGGGAACTCC AGGCGGCATG GAGGAACGGC TTTGACCAGT GCGATTACGG GTGGCTGTCG 
GATGCCAGCG TGCGCCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACAGGCT TCCCTCCCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACGACCTGAT CGCTGCAAAA TGAACCCGTG CCTTAACGGA 
GGCACCTGTT ATCCTACTGA AACTTCCTAC GTATGCACCT GTGTGCCAGG ATACAGCGGA 
GACCAGTGTG AACTTGATTT TGATGAATGT CACTCTAATC CCTGTCGTAA TGGAGCCACT 
TGTGTTGATG GTTTTAACAC ATTCAGGTGC CTCTGCCTTC CAAGTTATGT TGGTGCACTT 
TGTGAGCAAG ATACCGAGAC ATGTGACTAT GGCTGGCACA AATTCCAAGG GCAGTGCTAC 
AAATACTTTG CCCATCGACG CACATGGGAT GCAGCTGAAC GGGAATGCCG TCTGCAGGGT 
GCCCATCTCA CAAGCATCCT GTCTCACGAA GAACAAATGT TTGTTAATCG TGTGGGCCAT 
GATTATCAGT GGATAGGCCT CAATGACAAG ATGTTTGAGC ATGACTTCCG TTGGACTGAT 
GGCAGCACAC TGCAATACGA GAATTGGAGA CCCAACCAGC CAGACAGCTT CTTTTCTGCT 
GGAGAAGACT GTGTTGTAAT CATTTGGCAT GAGAATGGCC AGTGGAATGA TGTTCCCTGC 
AATTACCATC TCACCTATAC GTGCAAGAAA GGAACAGTCG CTTGCGGCCA GCCCCCTGTT 
GTAGAAAATG CCAAGACCTT TGGAAAGATG AAACCTCGTT ATGAAATCAA CTCCCTGATT 
AGATACCACT GCAAAGATGG TTTCATTCAA CGTCACCTTC CAACTATCCG GTGCTTAGGA 
AATGGAAGAT GGGCTATACC TAAAATTACC TGCATGAACC CATCTGCATA CCAAAGGACT 
TATTCTATGA AATACTTTAA AAATTCCTCA TCAGCAAAGG ACAATTCAAT AAATACATCC 
AAACATGATC ATCGTTGGAG CCGGAGGTGG CAGGAGTCGA GGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCAN ( NM_001126336.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001119808.1 Homo sapiens versican (VCAN), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22613
Clone ID Related Accession (Same CDS sequence)	NM_001126336.2 , NM_001126336.3
Accession Version	NM_001126336.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	versican core protein isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA361670.1, D32039.1, BC050524.1, DT217515.1 and AC026696.5. On May 31, 2019 this sequence version replaced NM_001126336.2. Transcript Variant: This variant (2), also known as PG-M(V3), omits two coding exons, compared to variant 1. This results in a shorter protein (isoform 2) with no chondroitin sulfate attachment domain. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.71064.1, D32039.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGTTCATAA ATATAAAGAG CATCTTATGG ATGTGTTCAA CCTTAATAGT AACCCATGCG 
CTACATAAAG TCAAAGTGGG AAAAAGCCCA CCGGTGAGGG GCTCCCTCTC TGGAAAAGTC 
AGCCTACCTT GTCATTTTTC AACGATGCCT ACTTTGCCAC CCAGTTACAA CACCAGTGAA 
TTTCTCCGCA TCAAATGGTC TAAGATTGAA GTGGACAAAA ATGGAAAAGA TTTGAAAGAG 
ACTACTGTCC TTGTGGCCCA AAATGGAAAT ATCAAGATTG GTCAGGACTA CAAAGGGAGA 
GTGTCTGTGC CCACACATCC CGAGGCTGTG GGCGATGCCT CCCTCACTGT GGTCAAGCTG 
CTGGCAAGTG ATGCGGGTCT TTACCGCTGT GACGTCATGT ACGGGATTGA AGACACACAA 
GACACGGTGT CACTGACTGT GGATGGGGTT GTGTTTCACT ACAGGGCGGC AACCAGCAGG 
TACACACTGA ATTTTGAGGC TGCTCAGAAG GCTTGTTTGG ACGTTGGGGC AGTCATAGCA 
ACTCCAGAGC AGCTCTTTGC TGCCTATGAA GATGGATTTG AGCAGTGTGA CGCAGGCTGG 
CTGGCTGATC AGACTGTCAG ATATCCCATC CGGGCTCCCA GAGTAGGCTG TTATGGAGAT 
AAGATGGGAA AGGCAGGAGT CAGGACTTAT GGATTCCGTT CTCCCCAGGA AACTTACGAT 
GTGTATTGTT ATGTGGATCA TCTGGATGGT GATGTGTTCC ACCTCACTGT CCCCAGTAAA 
TTCACCTTCG AGGAGGCTGC AAAAGAGTGT GAAAACCAGG ATGCCAGGCT GGCAACAGTG 
GGGGAACTCC AGGCGGCATG GAGGAACGGC TTTGACCAGT GCGATTACGG GTGGCTGTCG 
GATGCCAGCG TGCGCCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACAGGCT TCCCTCCCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACGACCTGAT CGCTGCAAAA TGAACCCGTG CCTTAACGGA 
GGCACCTGTT ATCCTACTGA AACTTCCTAC GTATGCACCT GTGTGCCAGG ATACAGCGGA 
GACCAGTGTG AACTTGATTT TGATGAATGT CACTCTAATC CCTGTCGTAA TGGAGCCACT 
TGTGTTGATG GTTTTAACAC ATTCAGGTGC CTCTGCCTTC CAAGTTATGT TGGTGCACTT 
TGTGAGCAAG ATACCGAGAC ATGTGACTAT GGCTGGCACA AATTCCAAGG GCAGTGCTAC 
AAATACTTTG CCCATCGACG CACATGGGAT GCAGCTGAAC GGGAATGCCG TCTGCAGGGT 
GCCCATCTCA CAAGCATCCT GTCTCACGAA GAACAAATGT TTGTTAATCG TGTGGGCCAT 
GATTATCAGT GGATAGGCCT CAATGACAAG ATGTTTGAGC ATGACTTCCG TTGGACTGAT 
GGCAGCACAC TGCAATACGA GAATTGGAGA CCCAACCAGC CAGACAGCTT CTTTTCTGCT 
GGAGAAGACT GTGTTGTAAT CATTTGGCAT GAGAATGGCC AGTGGAATGA TGTTCCCTGC 
AATTACCATC TCACCTATAC GTGCAAGAAA GGAACAGTCG CTTGCGGCCA GCCCCCTGTT 
GTAGAAAATG CCAAGACCTT TGGAAAGATG AAACCTCGTT ATGAAATCAA CTCCCTGATT 
AGATACCACT GCAAAGATGG TTTCATTCAA CGTCACCTTC CAACTATCCG GTGCTTAGGA 
AATGGAAGAT GGGCTATACC TAAAATTACC TGCATGAACC CATCTGCATA CCAAAGGACT 
TATTCTATGA AATACTTTAA AAATTCCTCA TCAGCAAAGG ACAATTCAAT AAATACATCC 
AAACATGATC ATCGTTGGAG CCGGAGGTGG CAGGAGTCGA GGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119808.1
CDS	287..2254
Translation	MFINIKSILWMCSTLIVTHALHKVKVGKSPPVRGSLSGKVSLPCHFSTMPTLPPSYNTSEFLRIKWSKIEVDKNGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEAVGDASLTVVKLLASDAGLYRCDVMYGIEDTQDTVSLTVDGVVFHYRAATSRYTLNFEAAQKACLDVGAVIATPEQLFAAYEDGFEQCDAGWLADQTVRYPIRAPRVGCYGDKMGKAGVRTYGFRSPQETYDVYCYVDHLDGDVFHLTVPSKFTFEEAAKECENQDARLATVGELQAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPPPDSRFDAYCFKRPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDQCELDFDECHSNPCRNGATCVDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAIPKITCMNPSAYQRTYSMKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Target ORF information:

RefSeq Version	NM_001126336.3
Organism	Homo sapiens(human)
Definition	Homo sapiens versican (VCAN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126336.3

ORF Insert Sequence:

ATGTTCATAA ATATAAAGAG CATCTTATGG ATGTGTTCAA CCTTAATAGT AACCCATGCG 
CTACATAAAG TCAAAGTGGG AAAAAGCCCA CCGGTGAGGG GCTCCCTCTC TGGAAAAGTC 
AGCCTACCTT GTCATTTTTC AACGATGCCT ACTTTGCCAC CCAGTTACAA CACCAGTGAA 
TTTCTCCGCA TCAAATGGTC TAAGATTGAA GTGGACAAAA ATGGAAAAGA TTTGAAAGAG 
ACTACTGTCC TTGTGGCCCA AAATGGAAAT ATCAAGATTG GTCAGGACTA CAAAGGGAGA 
GTGTCTGTGC CCACACATCC CGAGGCTGTG GGCGATGCCT CCCTCACTGT GGTCAAGCTG 
CTGGCAAGTG ATGCGGGTCT TTACCGCTGT GACGTCATGT ACGGGATTGA AGACACACAA 
GACACGGTGT CACTGACTGT GGATGGGGTT GTGTTTCACT ACAGGGCGGC AACCAGCAGG 
TACACACTGA ATTTTGAGGC TGCTCAGAAG GCTTGTTTGG ACGTTGGGGC AGTCATAGCA 
ACTCCAGAGC AGCTCTTTGC TGCCTATGAA GATGGATTTG AGCAGTGTGA CGCAGGCTGG 
CTGGCTGATC AGACTGTCAG ATATCCCATC CGGGCTCCCA GAGTAGGCTG TTATGGAGAT 
AAGATGGGAA AGGCAGGAGT CAGGACTTAT GGATTCCGTT CTCCCCAGGA AACTTACGAT 
GTGTATTGTT ATGTGGATCA TCTGGATGGT GATGTGTTCC ACCTCACTGT CCCCAGTAAA 
TTCACCTTCG AGGAGGCTGC AAAAGAGTGT GAAAACCAGG ATGCCAGGCT GGCAACAGTG 
GGGGAACTCC AGGCGGCATG GAGGAACGGC TTTGACCAGT GCGATTACGG GTGGCTGTCG 
GATGCCAGCG TGCGCCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACAGGCT TCCCTCCCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACGACCTGAT CGCTGCAAAA TGAACCCGTG CCTTAACGGA 
GGCACCTGTT ATCCTACTGA AACTTCCTAC GTATGCACCT GTGTGCCAGG ATACAGCGGA 
GACCAGTGTG AACTTGATTT TGATGAATGT CACTCTAATC CCTGTCGTAA TGGAGCCACT 
TGTGTTGATG GTTTTAACAC ATTCAGGTGC CTCTGCCTTC CAAGTTATGT TGGTGCACTT 
TGTGAGCAAG ATACCGAGAC ATGTGACTAT GGCTGGCACA AATTCCAAGG GCAGTGCTAC 
AAATACTTTG CCCATCGACG CACATGGGAT GCAGCTGAAC GGGAATGCCG TCTGCAGGGT 
GCCCATCTCA CAAGCATCCT GTCTCACGAA GAACAAATGT TTGTTAATCG TGTGGGCCAT 
GATTATCAGT GGATAGGCCT CAATGACAAG ATGTTTGAGC ATGACTTCCG TTGGACTGAT 
GGCAGCACAC TGCAATACGA GAATTGGAGA CCCAACCAGC CAGACAGCTT CTTTTCTGCT 
GGAGAAGACT GTGTTGTAAT CATTTGGCAT GAGAATGGCC AGTGGAATGA TGTTCCCTGC 
AATTACCATC TCACCTATAC GTGCAAGAAA GGAACAGTCG CTTGCGGCCA GCCCCCTGTT 
GTAGAAAATG CCAAGACCTT TGGAAAGATG AAACCTCGTT ATGAAATCAA CTCCCTGATT 
AGATACCACT GCAAAGATGG TTTCATTCAA CGTCACCTTC CAACTATCCG GTGCTTAGGA 
AATGGAAGAT GGGCTATACC TAAAATTACC TGCATGAACC CATCTGCATA CCAAAGGACT 
TATTCTATGA AATACTTTAA AAATTCCTCA TCAGCAAAGG ACAATTCAAT AAATACATCC 
AAACATGATC ATCGTTGGAG CCGGAGGTGG CAGGAGTCGA GGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCAN ( NM_001164098.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157570.1 Homo sapiens versican (VCAN), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22135
Clone ID Related Accession (Same CDS sequence)	NM_001164098.1
Accession Version	NM_001164098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	versican core protein isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA361670.1, U26555.1, AA320958.1 and AC026696.5. Transcript Variant: This variant (4), also known as PG-M(V2), lacks an exon in the coding region, compared to variant 1. This results in a shorter protein (isoform 4), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.35659.1, SRR1803615.42023.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END##

ATGTTCATAA ATATAAAGAG CATCTTATGG ATGTGTTCAA CCTTAATAGT AACCCATGCG 
CTACATAAAG TCAAAGTGGG AAAAAGCCCA CCGGTGAGGG GCTCCCTCTC TGGAAAAGTC 
AGCCTACCTT GTCATTTTTC AACGATGCCT ACTTTGCCAC CCAGTTACAA CACCAGTGAA 
TTTCTCCGCA TCAAATGGTC TAAGATTGAA GTGGACAAAA ATGGAAAAGA TTTGAAAGAG 
ACTACTGTCC TTGTGGCCCA AAATGGAAAT ATCAAGATTG GTCAGGACTA CAAAGGGAGA 
GTGTCTGTGC CCACACATCC CGAGGCTGTG GGCGATGCCT CCCTCACTGT GGTCAAGCTG 
CTGGCAAGTG ATGCGGGTCT TTACCGCTGT GACGTCATGT ACGGGATTGA AGACACACAA 
GACACGGTGT CACTGACTGT GGATGGGGTT GTGTTTCACT ACAGGGCGGC AACCAGCAGG 
TACACACTGA ATTTTGAGGC TGCTCAGAAG GCTTGTTTGG ACGTTGGGGC AGTCATAGCA 
ACTCCAGAGC AGCTCTTTGC TGCCTATGAA GATGGATTTG AGCAGTGTGA CGCAGGCTGG 
CTGGCTGATC AGACTGTCAG ATATCCCATC CGGGCTCCCA GAGTAGGCTG TTATGGAGAT 
AAGATGGGAA AGGCAGGAGT CAGGACTTAT GGATTCCGTT CTCCCCAGGA AACTTACGAT 
GTGTATTGTT ATGTGGATCA TCTGGATGGT GATGTGTTCC ACCTCACTGT CCCCAGTAAA 
TTCACCTTCG AGGAGGCTGC AAAAGAGTGT GAAAACCAGG ATGCCAGGCT GGCAACAGTG 
GGGGAACTCC AGGCGGCATG GAGGAACGGC TTTGACCAGT GCGATTACGG GTGGCTGTCG 
GATGCCAGCG TGCGCCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACAGGCT TCCCTCCCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACCTAAAGAG GCTACAACCA TCGATTTGAG TATCCTCGCA 
GAAACTGCAT CACCCAGTTT ATCCAAAGAA CCACAAATGG TTTCTGATAG AACTACACCA 
ATCATCCCTT TAGTTGATGA ATTACCTGTC ATTCCAACAG AGTTCCCTCC CGTGGGAAAT 
ATTGTCAGTT TTGAACAGAA AGCCACAGTC CAACCTCAGG CTATCACAGA TAGTTTAGCC 
ACCAAATTAC CCACACCTAC TGGCAGTACC AAGAAGCCCT GGGATATGGA TGACTACTCA 
CCTTCTGCTT CAGGACCTCT TGGAAAGCTA GACATATCAG AAATTAAGGA AGAAGTGCTC 
CAGAGTACAA CTGGCGTCTC TCATTATGCT ACGGATTCAT GGGATGGTGT CGTGGAAGAT 
AAACAAACAC AAGAATCGGT TACACAGATT GAACAAATAG AAGTGGGTCC TTTGGTAACA 
TCTATGGAAA TCTTAAAGCA CATTCCTTCC AAGGAATTCC CTGTAACTGA AACACCATTG 
GTAACTGCAA GAATGATCCT GGAATCCAAA ACTGAAAAGA AAATGGTAAG CACTGTTTCT 
GAATTGGTAA CCACAGGTCA CTATGGATTC ACCTTGGGAG AAGAGGATGA TGAAGACAGA 
ACACTTACAG TTGGATCTGA TGAGAGCACC TTGATCTTTG ACCAAATTCC TGAAGTCATT 
ACGGTGTCAA AGACTTCAGA AGACACCATC CACACTCATT TAGAAGACTT GGAGTCAGTC 
TCAGCATCCA CAACTGTTTC CCCTTTAATT ATGCCTGATA ATAATGGATC ATCCATGGAT 
GACTGGGAAG AGAGACAAAC TAGTGGTAGG ATAACGGAAG AGTTTCTTGG CAAATATCTG 
TCTACTACAC CTTTTCCATC ACAGCATCGT ACAGAAATAG AATTGTTTCC TTATTCTGGT 
GATAAAATAT TAGTAGAGGG AATTTCCACA GTTATTTATC CTTCTCTACA AACAGAAATG 
ACACATAGAA GAGAAAGAAC AGAAACACTA ATACCAGAGA TGAGAACAGA TACTTATACA 
GATGAAATAC AAGAAGAGAT CACTAAAAGT CCATTTATGG GAAAAACAGA AGAAGAAGTC 
TTCTCTGGGA TGAAACTCTC TACATCTCTC TCAGAGCCAA TTCATGTTAC AGAGTCTTCT 
GTGGAAATGA CCAAGTCTTT TGATTTCCCA ACATTGATAA CAAAGTTAAG TGCAGAGCCA 
ACAGAAGTAA GAGATATGGA GGAAGACTTT ACAGCAACTC CAGGTACTAC AAAATATGAT 
GAAAATATTA CAACAGTGCT TTTGGCCCAT GGTACTTTAA GTGTTGAAGC AGCCACTGTA 
TCAAAATGGT CATGGGATGA AGATAATACA ACATCCAAGC CTTTAGAGTC TACAGAACCT 
TCAGCCTCTT CAAAATTGCC CCCTGCCTTA CTCACAACTG TGGGGATGAA TGGAAAGGAT 
AAAGACATCC CAAGTTTCAC TGAAGATGGA GCAGATGAAT TTACTCTTAT TCCAGATAGT 
ACTCAAAAGC AGTTAGAGGA GGTTACTGAT GAAGACATAG CAGCCCATGG AAAATTCACA 
ATTAGATTTC AGCCAACTAC ATCAACTGGT ATTGCAGAAA AGTCAACTTT GAGAGATTCT 
ACAACTGAAG AAAAAGTTCC ACCTATCACA AGCACTGAAG GCCAAGTTTA TGCAACCATG 
GAAGGAAGTG CTTTGGGTGA AGTAGAAGAT GTGGACCTCT CTAAGCCAGT ATCTACTGTT 
CCCCAATTTG CACACACTTC AGAGGTGGAA GGATTAGCAT TTGTTAGTTA TAGTAGCACC 
CAAGAGCCTA CTACTTATGT AGACTCTTCC CATACCATTC CTCTTTCTGT AATTCCCAAG 
ACAGACTGGG GAGTGTTAGT ACCTTCTGTT CCATCAGAAG ATGAAGTTCT AGGTGAACCC 
TCTCAAGACA TACTTGTCAT TGATCAGACT CGCCTTGAAG CGACTATTTC TCCAGAAACT 
ATGAGAACAA CAAAAATCAC AGAGGGAACA ACTCAGGAAG AATTCCCTTG GAAAGAACAG 
ACTGCAGAGA AACCAGTTCC TGCTCTCAGT TCTACAGCTT GGACTCCCAA GGAGGCAGTA 
ACACCACTGG ATGAACAAGA GGGCGATGGA TCAGCATATA CAGTCTCTGA AGATGAATTG 
TTGACAGGTT CTGAGAGGGT CCCAGTTTTA GAAACAACTC CAGTTGGAAA AATTGATCAC 
AGTGTGTCTT ATCCACCAGG TGCTGTAACT GAGCACAAAG TGAAAACAGA TGAAGTGGTA 
ACACTAACAC CACGCATTGG GCCAAAAGTA TCTTTAAGTC CAGGGCCTGA ACAAAAATAT 
GAAACAGAAG GTAGTAGTAC AACAGGATTT ACATCATCTT TGAGTCCTTT TAGTACCCAC 
ATTACCCAGC TTATGGAAGA AACCACTACT GAGAAAACAT CCCTAGAGGA TATTGATTTA 
GGCTCAGGAT TATTTGAAAA GCCCAAAGCC ACAGAACTCA TAGAATTTTC AACAATCAAA 
GTCACAGTTC CAAGTGATAT TACCACTGCC TTCAGTTCAG TAGACAGACT TCACACAACT 
TCAGCATTCA AGCCATCTTC CGCGATCACT AAGAAACCAC CTCTCATCGA CAGGGAACCT 
GGTGAAGAAA CAACCAGTGA CATGGTAATC ATTGGAGAAT CAACATCTCA TGTTCCTCCC 
ACTACCCTTG AAGATATTGT AGCCAAGGAA ACAGAAACCG ATATTGATAG AGAGTATTTC 
ACGACTTCAA GTCCTCCTGC TACACAGCCA ACAAGACCAC CCACTGTGGA AGACAAAGAG 
GCCTTTGGAC CTCAGGCGCT TTCTACGCCA CAGCCCCCAG CAAGCACAAA ATTTCACCCT 
GACATTAATG TTTATATTAT TGAGGTCAGA GAAAATAAGA CAGGACCTGA TCGCTGCAAA 
ATGAACCCGT GCCTTAACGG AGGCACCTGT TATCCTACTG AAACTTCCTA CGTATGCACC 
TGTGTGCCAG GATACAGCGG AGACCAGTGT GAACTTGATT TTGATGAATG TCACTCTAAT 
CCCTGTCGTA ATGGAGCCAC TTGTGTTGAT GGTTTTAACA CATTCAGGTG CCTCTGCCTT 
CCAAGTTATG TTGGTGCACT TTGTGAGCAA GATACCGAGA CATGTGACTA TGGCTGGCAC 
AAATTCCAAG GGCAGTGCTA CAAATACTTT GCCCATCGAC GCACATGGGA TGCAGCTGAA 
CGGGAATGCC GTCTGCAGGG TGCCCATCTC ACAAGCATCC TGTCTCACGA AGAACAAATG 
TTTGTTAATC GTGTGGGCCA TGATTATCAG TGGATAGGCC TCAATGACAA GATGTTTGAG 
CATGACTTCC GTTGGACTGA TGGCAGCACA CTGCAATACG AGAATTGGAG ACCCAACCAG 
CCAGACAGCT TCTTTTCTGC TGGAGAAGAC TGTGTTGTAA TCATTTGGCA TGAGAATGGC 
CAGTGGAATG ATGTTCCCTG CAATTACCAT CTCACCTATA CGTGCAAGAA AGGAACAGTC 
GCTTGCGGCC AGCCCCCTGT TGTAGAAAAT GCCAAGACCT TTGGAAAGAT GAAACCTCGT 
TATGAAATCA ACTCCCTGAT TAGATACCAC TGCAAAGATG GTTTCATTCA ACGTCACCTT 
CCAACTATCC GGTGCTTAGG AAATGGAAGA TGGGCTATAC CTAAAATTAC CTGCATGAAC 
CCATCTGCAT ACCAAAGGAC TTATTCTATG AAATACTTTA AAAATTCCTC ATCAGCAAAG 
GACAATTCAA TAAATACATC CAAACATGAT CATCGTTGGA GCCGGAGGTG GCAGGAGTCG 
AGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157570.1
CDS	357..5285
Translation	MFINIKSILWMCSTLIVTHALHKVKVGKSPPVRGSLSGKVSLPCHFSTMPTLPPSYNTSEFLRIKWSKIEVDKNGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEAVGDASLTVVKLLASDAGLYRCDVMYGIEDTQDTVSLTVDGVVFHYRAATSRYTLNFEAAQKACLDVGAVIATPEQLFAAYEDGFEQCDAGWLADQTVRYPIRAPRVGCYGDKMGKAGVRTYGFRSPQETYDVYCYVDHLDGDVFHLTVPSKFTFEEAAKECENQDARLATVGELQAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPPPDSRFDAYCFKPKEATTIDLSILAETASPSLSKEPQMVSDRTTPIIPLVDELPVIPTEFPPVGNIVSFEQKATVQPQAITDSLATKLPTPTGSTKKPWDMDDYSPSASGPLGKLDISEIKEEVLQSTTGVSHYATDSWDGVVEDKQTQESVTQIEQIEVGPLVTSMEILKHIPSKEFPVTETPLVTARMILESKTEKKMVSTVSELVTTGHYGFTLGEEDDEDRTLTVGSDESTLIFDQIPEVITVSKTSEDTIHTHLEDLESVSASTTVSPLIMPDNNGSSMDDWEERQTSGRITEEFLGKYLSTTPFPSQHRTEIELFPYSGDKILVEGISTVIYPSLQTEMTHRRERTETLIPEMRTDTYTDEIQEEITKSPFMGKTEEEVFSGMKLSTSLSEPIHVTESSVEMTKSFDFPTLITKLSAEPTEVRDMEEDFTATPGTTKYDENITTVLLAHGTLSVEAATVSKWSWDEDNTTSKPLESTEPSASSKLPPALLTTVGMNGKDKDIPSFTEDGADEFTLIPDSTQKQLEEVTDEDIAAHGKFTIRFQPTTSTGIAEKSTLRDSTTEEKVPPITSTEGQVYATMEGSALGEVEDVDLSKPVSTVPQFAHTSEVEGLAFVSYSSTQEPTTYVDSSHTIPLSVIPKTDWGVLVPSVPSEDEVLGEPSQDILVIDQTRLEATISPETMRTTKITEGTTQEEFPWKEQTAEKPVPALSSTAWTPKEAVTPLDEQEGDGSAYTVSEDELLTGSERVPVLETTPVGKIDHSVSYPPGAVTEHKVKTDEVVTLTPRIGPKVSLSPGPEQKYETEGSSTTGFTSSLSPFSTHITQLMEETTTEKTSLEDIDLGSGLFEKPKATELIEFSTIKVTVPSDITTAFSSVDRLHTTSAFKPSSAITKKPPLIDREPGEETTSDMVIIGESTSHVPPTTLEDIVAKETETDIDREYFTTSSPPATQPTRPPTVEDKEAFGPQALSTPQPPASTKFHPDINVYIIEVRENKTGPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDQCELDFDECHSNPCRNGATCVDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAIPKITCMNPSAYQRTYSMKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Target ORF information:

RefSeq Version	NM_001164098.1
Organism	Homo sapiens(human)
Definition	Homo sapiens versican (VCAN), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164098.1

ORF Insert Sequence:

ATGTTCATAA ATATAAAGAG CATCTTATGG ATGTGTTCAA CCTTAATAGT AACCCATGCG 
CTACATAAAG TCAAAGTGGG AAAAAGCCCA CCGGTGAGGG GCTCCCTCTC TGGAAAAGTC 
AGCCTACCTT GTCATTTTTC AACGATGCCT ACTTTGCCAC CCAGTTACAA CACCAGTGAA 
TTTCTCCGCA TCAAATGGTC TAAGATTGAA GTGGACAAAA ATGGAAAAGA TTTGAAAGAG 
ACTACTGTCC TTGTGGCCCA AAATGGAAAT ATCAAGATTG GTCAGGACTA CAAAGGGAGA 
GTGTCTGTGC CCACACATCC CGAGGCTGTG GGCGATGCCT CCCTCACTGT GGTCAAGCTG 
CTGGCAAGTG ATGCGGGTCT TTACCGCTGT GACGTCATGT ACGGGATTGA AGACACACAA 
GACACGGTGT CACTGACTGT GGATGGGGTT GTGTTTCACT ACAGGGCGGC AACCAGCAGG 
TACACACTGA ATTTTGAGGC TGCTCAGAAG GCTTGTTTGG ACGTTGGGGC AGTCATAGCA 
ACTCCAGAGC AGCTCTTTGC TGCCTATGAA GATGGATTTG AGCAGTGTGA CGCAGGCTGG 
CTGGCTGATC AGACTGTCAG ATATCCCATC CGGGCTCCCA GAGTAGGCTG TTATGGAGAT 
AAGATGGGAA AGGCAGGAGT CAGGACTTAT GGATTCCGTT CTCCCCAGGA AACTTACGAT 
GTGTATTGTT ATGTGGATCA TCTGGATGGT GATGTGTTCC ACCTCACTGT CCCCAGTAAA 
TTCACCTTCG AGGAGGCTGC AAAAGAGTGT GAAAACCAGG ATGCCAGGCT GGCAACAGTG 
GGGGAACTCC AGGCGGCATG GAGGAACGGC TTTGACCAGT GCGATTACGG GTGGCTGTCG 
GATGCCAGCG TGCGCCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACAGGCT TCCCTCCCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACCTAAAGAG GCTACAACCA TCGATTTGAG TATCCTCGCA 
GAAACTGCAT CACCCAGTTT ATCCAAAGAA CCACAAATGG TTTCTGATAG AACTACACCA 
ATCATCCCTT TAGTTGATGA ATTACCTGTC ATTCCAACAG AGTTCCCTCC CGTGGGAAAT 
ATTGTCAGTT TTGAACAGAA AGCCACAGTC CAACCTCAGG CTATCACAGA TAGTTTAGCC 
ACCAAATTAC CCACACCTAC TGGCAGTACC AAGAAGCCCT GGGATATGGA TGACTACTCA 
CCTTCTGCTT CAGGACCTCT TGGAAAGCTA GACATATCAG AAATTAAGGA AGAAGTGCTC 
CAGAGTACAA CTGGCGTCTC TCATTATGCT ACGGATTCAT GGGATGGTGT CGTGGAAGAT 
AAACAAACAC AAGAATCGGT TACACAGATT GAACAAATAG AAGTGGGTCC TTTGGTAACA 
TCTATGGAAA TCTTAAAGCA CATTCCTTCC AAGGAATTCC CTGTAACTGA AACACCATTG 
GTAACTGCAA GAATGATCCT GGAATCCAAA ACTGAAAAGA AAATGGTAAG CACTGTTTCT 
GAATTGGTAA CCACAGGTCA CTATGGATTC ACCTTGGGAG AAGAGGATGA TGAAGACAGA 
ACACTTACAG TTGGATCTGA TGAGAGCACC TTGATCTTTG ACCAAATTCC TGAAGTCATT 
ACGGTGTCAA AGACTTCAGA AGACACCATC CACACTCATT TAGAAGACTT GGAGTCAGTC 
TCAGCATCCA CAACTGTTTC CCCTTTAATT ATGCCTGATA ATAATGGATC ATCCATGGAT 
GACTGGGAAG AGAGACAAAC TAGTGGTAGG ATAACGGAAG AGTTTCTTGG CAAATATCTG 
TCTACTACAC CTTTTCCATC ACAGCATCGT ACAGAAATAG AATTGTTTCC TTATTCTGGT 
GATAAAATAT TAGTAGAGGG AATTTCCACA GTTATTTATC CTTCTCTACA AACAGAAATG 
ACACATAGAA GAGAAAGAAC AGAAACACTA ATACCAGAGA TGAGAACAGA TACTTATACA 
GATGAAATAC AAGAAGAGAT CACTAAAAGT CCATTTATGG GAAAAACAGA AGAAGAAGTC 
TTCTCTGGGA TGAAACTCTC TACATCTCTC TCAGAGCCAA TTCATGTTAC AGAGTCTTCT 
GTGGAAATGA CCAAGTCTTT TGATTTCCCA ACATTGATAA CAAAGTTAAG TGCAGAGCCA 
ACAGAAGTAA GAGATATGGA GGAAGACTTT ACAGCAACTC CAGGTACTAC AAAATATGAT 
GAAAATATTA CAACAGTGCT TTTGGCCCAT GGTACTTTAA GTGTTGAAGC AGCCACTGTA 
TCAAAATGGT CATGGGATGA AGATAATACA ACATCCAAGC CTTTAGAGTC TACAGAACCT 
TCAGCCTCTT CAAAATTGCC CCCTGCCTTA CTCACAACTG TGGGGATGAA TGGAAAGGAT 
AAAGACATCC CAAGTTTCAC TGAAGATGGA GCAGATGAAT TTACTCTTAT TCCAGATAGT 
ACTCAAAAGC AGTTAGAGGA GGTTACTGAT GAAGACATAG CAGCCCATGG AAAATTCACA 
ATTAGATTTC AGCCAACTAC ATCAACTGGT ATTGCAGAAA AGTCAACTTT GAGAGATTCT 
ACAACTGAAG AAAAAGTTCC ACCTATCACA AGCACTGAAG GCCAAGTTTA TGCAACCATG 
GAAGGAAGTG CTTTGGGTGA AGTAGAAGAT GTGGACCTCT CTAAGCCAGT ATCTACTGTT 
CCCCAATTTG CACACACTTC AGAGGTGGAA GGATTAGCAT TTGTTAGTTA TAGTAGCACC 
CAAGAGCCTA CTACTTATGT AGACTCTTCC CATACCATTC CTCTTTCTGT AATTCCCAAG 
ACAGACTGGG GAGTGTTAGT ACCTTCTGTT CCATCAGAAG ATGAAGTTCT AGGTGAACCC 
TCTCAAGACA TACTTGTCAT TGATCAGACT CGCCTTGAAG CGACTATTTC TCCAGAAACT 
ATGAGAACAA CAAAAATCAC AGAGGGAACA ACTCAGGAAG AATTCCCTTG GAAAGAACAG 
ACTGCAGAGA AACCAGTTCC TGCTCTCAGT TCTACAGCTT GGACTCCCAA GGAGGCAGTA 
ACACCACTGG ATGAACAAGA GGGCGATGGA TCAGCATATA CAGTCTCTGA AGATGAATTG 
TTGACAGGTT CTGAGAGGGT CCCAGTTTTA GAAACAACTC CAGTTGGAAA AATTGATCAC 
AGTGTGTCTT ATCCACCAGG TGCTGTAACT GAGCACAAAG TGAAAACAGA TGAAGTGGTA 
ACACTAACAC CACGCATTGG GCCAAAAGTA TCTTTAAGTC CAGGGCCTGA ACAAAAATAT 
GAAACAGAAG GTAGTAGTAC AACAGGATTT ACATCATCTT TGAGTCCTTT TAGTACCCAC 
ATTACCCAGC TTATGGAAGA AACCACTACT GAGAAAACAT CCCTAGAGGA TATTGATTTA 
GGCTCAGGAT TATTTGAAAA GCCCAAAGCC ACAGAACTCA TAGAATTTTC AACAATCAAA 
GTCACAGTTC CAAGTGATAT TACCACTGCC TTCAGTTCAG TAGACAGACT TCACACAACT 
TCAGCATTCA AGCCATCTTC CGCGATCACT AAGAAACCAC CTCTCATCGA CAGGGAACCT 
GGTGAAGAAA CAACCAGTGA CATGGTAATC ATTGGAGAAT CAACATCTCA TGTTCCTCCC 
ACTACCCTTG AAGATATTGT AGCCAAGGAA ACAGAAACCG ATATTGATAG AGAGTATTTC 
ACGACTTCAA GTCCTCCTGC TACACAGCCA ACAAGACCAC CCACTGTGGA AGACAAAGAG 
GCCTTTGGAC CTCAGGCGCT TTCTACGCCA CAGCCCCCAG CAAGCACAAA ATTTCACCCT 
GACATTAATG TTTATATTAT TGAGGTCAGA GAAAATAAGA CAGGACCTGA TCGCTGCAAA 
ATGAACCCGT GCCTTAACGG AGGCACCTGT TATCCTACTG AAACTTCCTA CGTATGCACC 
TGTGTGCCAG GATACAGCGG AGACCAGTGT GAACTTGATT TTGATGAATG TCACTCTAAT 
CCCTGTCGTA ATGGAGCCAC TTGTGTTGAT GGTTTTAACA CATTCAGGTG CCTCTGCCTT 
CCAAGTTATG TTGGTGCACT TTGTGAGCAA GATACCGAGA CATGTGACTA TGGCTGGCAC 
AAATTCCAAG GGCAGTGCTA CAAATACTTT GCCCATCGAC GCACATGGGA TGCAGCTGAA 
CGGGAATGCC GTCTGCAGGG TGCCCATCTC ACAAGCATCC TGTCTCACGA AGAACAAATG 
TTTGTTAATC GTGTGGGCCA TGATTATCAG TGGATAGGCC TCAATGACAA GATGTTTGAG 
CATGACTTCC GTTGGACTGA TGGCAGCACA CTGCAATACG AGAATTGGAG ACCCAACCAG 
CCAGACAGCT TCTTTTCTGC TGGAGAAGAC TGTGTTGTAA TCATTTGGCA TGAGAATGGC 
CAGTGGAATG ATGTTCCCTG CAATTACCAT CTCACCTATA CGTGCAAGAA AGGAACAGTC 
GCTTGCGGCC AGCCCCCTGT TGTAGAAAAT GCCAAGACCT TTGGAAAGAT GAAACCTCGT 
TATGAAATCA ACTCCCTGAT TAGATACCAC TGCAAAGATG GTTTCATTCA ACGTCACCTT 
CCAACTATCC GGTGCTTAGG AAATGGAAGA TGGGCTATAC CTAAAATTAC CTGCATGAAC 
CCATCTGCAT ACCAAAGGAC TTATTCTATG AAATACTTTA AAAATTCCTC ATCAGCAAAG 
GACAATTCAA TAAATACATC CAAACATGAT CATCGTTGGA GCCGGAGGTG GCAGGAGTCG 
AGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCAN ( NM_001164097.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157569.1 Homo sapiens versican (VCAN), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22133
Clone ID Related Accession (Same CDS sequence)	NM_001164097.1
Accession Version	NM_001164097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	versican core protein isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA361670.1, X15998.1, BX641036.1, AA320958.1 and AC026696.5. Transcript Variant: This variant (3), also known as PG-M(V1), lacks an exon in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.40058.1, X15998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGTTCATAA ATATAAAGAG CATCTTATGG ATGTGTTCAA CCTTAATAGT AACCCATGCG 
CTACATAAAG TCAAAGTGGG AAAAAGCCCA CCGGTGAGGG GCTCCCTCTC TGGAAAAGTC 
AGCCTACCTT GTCATTTTTC AACGATGCCT ACTTTGCCAC CCAGTTACAA CACCAGTGAA 
TTTCTCCGCA TCAAATGGTC TAAGATTGAA GTGGACAAAA ATGGAAAAGA TTTGAAAGAG 
ACTACTGTCC TTGTGGCCCA AAATGGAAAT ATCAAGATTG GTCAGGACTA CAAAGGGAGA 
GTGTCTGTGC CCACACATCC CGAGGCTGTG GGCGATGCCT CCCTCACTGT GGTCAAGCTG 
CTGGCAAGTG ATGCGGGTCT TTACCGCTGT GACGTCATGT ACGGGATTGA AGACACACAA 
GACACGGTGT CACTGACTGT GGATGGGGTT GTGTTTCACT ACAGGGCGGC AACCAGCAGG 
TACACACTGA ATTTTGAGGC TGCTCAGAAG GCTTGTTTGG ACGTTGGGGC AGTCATAGCA 
ACTCCAGAGC AGCTCTTTGC TGCCTATGAA GATGGATTTG AGCAGTGTGA CGCAGGCTGG 
CTGGCTGATC AGACTGTCAG ATATCCCATC CGGGCTCCCA GAGTAGGCTG TTATGGAGAT 
AAGATGGGAA AGGCAGGAGT CAGGACTTAT GGATTCCGTT CTCCCCAGGA AACTTACGAT 
GTGTATTGTT ATGTGGATCA TCTGGATGGT GATGTGTTCC ACCTCACTGT CCCCAGTAAA 
TTCACCTTCG AGGAGGCTGC AAAAGAGTGT GAAAACCAGG ATGCCAGGCT GGCAACAGTG 
GGGGAACTCC AGGCGGCATG GAGGAACGGC TTTGACCAGT GCGATTACGG GTGGCTGTCG 
GATGCCAGCG TGCGCCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACAGGCT TCCCTCCCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACGTCGAATG AGTGATTTGA GTGTAATTGG TCATCCAATA 
GATTCAGAAT CTAAAGAAGA TGAACCTTGT AGTGAAGAAA CAGATCCAGT GCATGATCTA 
ATGGCTGAAA TTTTACCTGA ATTCCCTGAC ATAATTGAAA TAGACCTATA CCACAGTGAA 
GAAAATGAAG AAGAAGAAGA AGAGTGTGCA AATGCTACTG ATGTGACAAC CACCCCATCT 
GTGCAGTACA TAAATGGGAA GCATCTCGTT ACCACTGTGC CCAAGGACCC AGAAGCTGCA 
GAAGCTAGGC GTGGCCAGTT TGAAAGTGTT GCACCTTCTC AGAATTTCTC GGACAGCTCT 
GAAAGTGATA CTCATCCATT TGTAATAGCC AAAACGGAAT TGTCTACTGC TGTGCAACCT 
AATGAATCTA CAGAAACAAC TGAGTCTCTT GAAGTTACAT GGAAGCCTGA GACTTACCCT 
GAAACATCAG AACATTTTTC AGGTGGTGAG CCTGATGTTT TCCCCACAGT CCCATTCCAT 
GAGGAATTTG AAAGTGGAAC AGCCAAAAAA GGGGCAGAAT CAGTCACAGA GAGAGATACT 
GAAGTTGGTC ATCAGGCACA TGAACATACT GAACCTGTAT CTCTGTTTCC TGAAGAGTCT 
TCAGGAGAGA TTGCCATTGA CCAAGAATCT CAGAAAATAG CCTTTGCAAG GGCTACAGAA 
GTAACATTTG GTGAAGAGGT AGAAAAAAGT ACTTCTGTCA CATACACTCC CACTATAGTT 
CCAAGTTCTG CATCAGCATA TGTTTCAGAG GAAGAAGCAG TTACCCTAAT AGGAAATCCT 
TGGCCAGATG ACCTGTTGTC TACCAAAGAA AGCTGGGTAG AAGCAACTCC TAGACAAGTT 
GTAGAGCTCT CAGGGAGTTC TTCGATTCCA ATTACAGAAG GCTCTGGAGA AGCAGAAGAA 
GATGAAGATA CAATGTTCAC CATGGTAACT GATTTATCAC AGAGAAATAC TACTGATACA 
CTCATTACTT TAGACACTAG CAGGATAATC ACAGAAAGCT TTTTTGAGGT TCCTGCAACC 
ACCATTTATC CAGTTTCTGA ACAACCTTCT GCAAAAGTGG TGCCTACCAA GTTTGTAAGT 
GAAACAGACA CTTCTGAGTG GATTTCCAGT ACCACTGTTG AGGAAAAGAA AAGGAAGGAG 
GAGGAGGGAA CTACAGGTAC GGCTTCTACA TTTGAGGTAT ATTCATCTAC ACAGAGATCG 
GATCAATTAA TTTTACCCTT TGAATTAGAA AGTCCAAATG TAGCTACATC TAGTGATTCA 
GGTACCAGGA AAAGTTTTAT GTCCTTGACA ACACCAACAC AGTCTGAAAG GGAAATGACA 
GATTCTACTC CTGTCTTTAC AGAAACAAAT ACATTAGAAA ATTTGGGGGC ACAGACCACT 
GAGCACAGCA GTATCCATCA ACCTGGGGTT CAGGAAGGGC TGACCACTCT CCCACGTAGT 
CCTGCCTCTG TCTTTATGGA GCAGGGCTCT GGAGAAGCTG CTGCCGACCC AGAAACCACC 
ACTGTTTCTT CATTTTCATT AAACGTAGAG TATGCAATTC AAGCCGAAAA GGAAGTAGCT 
GGCACTTTGT CTCCGCATGT GGAAACTACA TTCTCCACTG AGCCAACAGG ACTGGTTTTG 
AGTACAGTAA TGGACAGAGT AGTTGCTGAA AATATAACCC AAACATCCAG GGAAATAGTG 
ATTTCAGAGC GATTAGGAGA ACCAAATTAT GGGGCAGAAA TAAGGGGCTT TTCCACAGGT 
TTTCCTTTGG AGGAAGATTT CAGTGGTGAC TTTAGAGAAT ACTCAACAGT GTCTCATCCC 
ATAGCAAAAG AAGAAACGGT AATGATGGAA GGCTCTGGAG ATGCAGCATT TAGGGACACC 
CAGACTTCAC CATCTACAGT ACCTACTTCA GTTCACATCA GTCACATATC TGACTCAGAA 
GGACCCAGTA GCACCATGGT CAGCACTTCA GCCTTCCCCT GGGAAGAGTT TACATCCTCA 
GCTGAGGGCT CAGGTGAGCA ACTGGTCACA GTCAGCAGCT CTGTTGTTCC AGTGCTTCCC 
AGTGCTGTGC AAAAGTTTTC TGGTACAGCT TCCTCCATTA TCGACGAAGG ATTGGGAGAA 
GTGGGTACTG TCAATGAAAT TGATAGAAGA TCCACCATTT TACCAACAGC AGAAGTGGAA 
GGTACGAAAG CTCCAGTAGA GAAGGAGGAA GTAAAGGTCA GTGGCACAGT TTCAACAAAC 
TTTCCCCAAA CTATAGAGCC AGCCAAATTA TGGTCTAGGC AAGAAGTCAA CCCTGTAAGA 
CAAGAAATTG AAAGTGAAAC AACATCAGAG GAACAAATTC AAGAAGAAAA GTCATTTGAA 
TCCCCTCAAA ACTCTCCTGC AACAGAACAA ACAATCTTTG ATTCACAGAC ATTTACTGAA 
ACTGAACTCA AAACCACAGA TTATTCTGTA CTAACAACAA AGAAAACTTA CAGTGATGAT 
AAAGAAATGA AGGAGGAAGA CACTTCTTTA GTTAACATGT CTACTCCAGA TCCAGATGCA 
AATGGCTTGG AATCTTACAC AACTCTCCCT GAAGCTACTG AAAAGTCACA TTTTTTCTTA 
GCTACTGCAT TAGTAACTGA ATCTATACCA GCTGAACATG TAGTCACAGA TTCACCAATC 
AAAAAGGAAG AAAGTACAAA ACATTTTCCG AAAGGCATGA GACCAACAAT TCAAGAGTCA 
GATACTGAGC TCTTATTCTC TGGACTGGGA TCAGGAGAAG AAGTTTTACC TACTCTACCA 
ACAGAGTCAG TGAATTTTAC TGAAGTGGAA CAAATCAATA ACACATTATA TCCCCACACT 
TCTCAAGTGG AAAGTACCTC AAGTGACAAA ATTGAAGACT TTAACAGAAT GGAAAATGTG 
GCAAAAGAAG TTGGACCACT CGTATCTCAA ACAGACATCT TTGAAGGTAG TGGGTCAGTA 
ACCAGCACAA CATTAATAGA AATTTTAAGT GACACTGGAG CAGAAGGACC CACGGTGGCA 
CCTCTCCCTT TCTCCACGGA CATCGGACAT CCTCAAAATC AGACTGTCAG GTGGGCAGAA 
GAAATCCAGA CTAGTAGACC ACAAACCATA ACTGAACAAG ACTCTAACAA GAATTCTTCA 
ACAGCAGAAA TTAACGAAAC AACAACCTCA TCTACTGATT TTCTGGCTAG AGCTTATGGT 
TTTGAAATGG CCAAAGAATT TGTTACATCA GCACCAAAAC CATCTGACTT GTATTATGAA 
CCTTCTGGAG AAGGATCTGG AGAAGTGGAT ATTGTTGATT CATTTCACAC TTCTGCAACT 
ACTCAGGCAA CCAGACAAGA AAGCAGCACC ACATTTGTTT CTGATGGGTC CCTGGAAAAA 
CATCCTGAGG TGCCAAGCGC TAAAGCTGTT ACTGCTGATG GATTCCCAAC AGTTTCAGTG 
ATGCTGCCTC TTCATTCAGA GCAGAACAAA AGCTCCCCTG ATCCAACTAG CACACTGTCA 
AATACAGTGT CATATGAGAG GTCCACAGAC GGTAGTTTCC AAGACCGTTT CAGGGAATTC 
GAGGATTCCA CCTTAAAACC TAACAGAAAA AAACCCACTG AAAATATTAT CATAGACCTG 
GACAAAGAGG ACAAGGATTT AATATTGACA ATTACAGAGA GTACCATCCT TGAAATTCTA 
CCTGAGCTGA CATCGGATAA AAATACTATC ATAGATATTG ATCATACTAA ACCTGTGTAT 
GAAGACATTC TTGGAATGCA AACAGATATA GATACAGAGG TACCATCAGA ACCACATGAC 
AGTAATGATG AAAGTAATGA TGACAGCACT CAAGTTCAAG AGATCTATGA GGCAGCTGTC 
AACCTTTCTT TAACTGAGGA AACATTTGAG GGCTCTGCTG ATGTTCTGGC TAGCTACACT 
CAGGCAACAC ATGATGAATC AATGACTTAT GAAGATAGAA GCCAACTAGA TCACATGGGC 
TTTCACTTCA CAACTGGGAT CCCTGCTCCT AGCACAGAAA CAGAATTAGA CGTTTTACTT 
CCCACGGCAA CATCCCTGCC AATTCCTCGT AAGTCTGCCA CAGTTATTCC AGAGATTGAA 
GGAATAAAAG CTGAAGCAAA AGCCCTGGAT GACATGTTTG AATCAAGCAC TTTGTCTGAT 
GGTCAAGCTA TTGCAGACCA AAGTGAAATA ATACCAACAT TGGGCCAATT TGAAAGGACT 
CAGGAGGAGT ATGAAGACAA AAAACATGCT GGTCCTTCTT TTCAGCCAGA ATTCTCTTCA 
GGAGCTGAGG AGGCATTAGT AGACCATACT CCCTATCTAA GTATTGCTAC TACCCACCTT 
ATGGATCAGA GTGTAACAGA GGTGCCTGAT GTGATGGAAG GATCCAATCC CCCATATTAC 
ACTGATACAA CATTAGCAGT TTCAACATTT GCGAAGTTGT CTTCTCAGAC ACCATCATCT 
CCCCTCACTA TCTACTCAGG CAGTGAAGCC TCTGGACACA CAGAGATCCC CCAGCCCAGT 
GCTCTGCCAG GAATAGACGT CGGCTCATCT GTAATGTCCC CACAGGATTC TTTTAAGGAA 
ATTCATGTAA ATATTGAAGC GACTTTCAAA CCATCAAGTG AGGAATACCT TCACATAACT 
GAGCCTCCCT CTTTATCTCC TGACACAAAA TTAGAACCTT CAGAAGATGA TGGTAAACCT 
GAGTTATTAG AAGAAATGGA AGCTTCTCCC ACAGAACTTA TTGCTGTGGA AGGAACTGAG 
ATTCTCCAAG ATTTCCAAAA CAAAACCGAT GGTCAAGTTT CTGGAGAAGC AATCAAGATG 
TTTCCCACCA TTAAAACACC TGAGGCTGGA ACTGTTATTA CAACTGCCGA TGAAATTGAA 
TTAGAAGGTG CTACACAGTG GCCACACTCT ACTTCTGCTT CTGCCACCTA TGGGGTCGAG 
GCAGGTGTGG TGCCTTGGCT AAGTCCACAG ACTTCTGAGA GGCCCACGCT TTCTTCTTCT 
CCAGAAATAA ACCCTGAAAC TCAAGCAGCT TTAATCAGAG GGCAGGATTC CACGATAGCA 
GCATCAGAAC AGCAAGTGGC AGCGAGAATT CTTGATTCCA ATGATCAGGC AACAGTAAAC 
CCTGTGGAAT TTAATACTGA GGTTGCAACA CCACCATTTT CCCTTCTGGA GACTTCTAAT 
GAAACAGATT TCCTGATTGG CATTAATGAA GAGTCAGTGG AAGGCACGGC AATCTATTTA 
CCAGGACCTG ATCGCTGCAA AATGAACCCG TGCCTTAACG GAGGCACCTG TTATCCTACT 
GAAACTTCCT ACGTATGCAC CTGTGTGCCA GGATACAGCG GAGACCAGTG TGAACTTGAT 
TTTGATGAAT GTCACTCTAA TCCCTGTCGT AATGGAGCCA CTTGTGTTGA TGGTTTTAAC 
ACATTCAGGT GCCTCTGCCT TCCAAGTTAT GTTGGTGCAC TTTGTGAGCA AGATACCGAG 
ACATGTGACT ATGGCTGGCA CAAATTCCAA GGGCAGTGCT ACAAATACTT TGCCCATCGA 
CGCACATGGG ATGCAGCTGA ACGGGAATGC CGTCTGCAGG GTGCCCATCT CACAAGCATC 
CTGTCTCACG AAGAACAAAT GTTTGTTAAT CGTGTGGGCC ATGATTATCA GTGGATAGGC 
CTCAATGACA AGATGTTTGA GCATGACTTC CGTTGGACTG ATGGCAGCAC ACTGCAATAC 
GAGAATTGGA GACCCAACCA GCCAGACAGC TTCTTTTCTG CTGGAGAAGA CTGTGTTGTA 
ATCATTTGGC ATGAGAATGG CCAGTGGAAT GATGTTCCCT GCAATTACCA TCTCACCTAT 
ACGTGCAAGA AAGGAACAGT CGCTTGCGGC CAGCCCCCTG TTGTAGAAAA TGCCAAGACC 
TTTGGAAAGA TGAAACCTCG TTATGAAATC AACTCCCTGA TTAGATACCA CTGCAAAGAT 
GGTTTCATTC AACGTCACCT TCCAACTATC CGGTGCTTAG GAAATGGAAG ATGGGCTATA 
CCTAAAATTA CCTGCATGAA CCCATCTGCA TACCAAAGGA CTTATTCTAT GAAATACTTT 
AAAAATTCCT CATCAGCAAA GGACAATTCA ATAAATACAT CCAAACATGA TCATCGTTGG 
AGCCGGAGGT GGCAGGAGTC GAGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157569.1
CDS	357..7586
Translation	MFINIKSILWMCSTLIVTHALHKVKVGKSPPVRGSLSGKVSLPCHFSTMPTLPPSYNTSEFLRIKWSKIEVDKNGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEAVGDASLTVVKLLASDAGLYRCDVMYGIEDTQDTVSLTVDGVVFHYRAATSRYTLNFEAAQKACLDVGAVIATPEQLFAAYEDGFEQCDAGWLADQTVRYPIRAPRVGCYGDKMGKAGVRTYGFRSPQETYDVYCYVDHLDGDVFHLTVPSKFTFEEAAKECENQDARLATVGELQAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPPPDSRFDAYCFKRRMSDLSVIGHPIDSESKEDEPCSEETDPVHDLMAEILPEFPDIIEIDLYHSEENEEEEEECANATDVTTTPSVQYINGKHLVTTVPKDPEAAEARRGQFESVAPSQNFSDSSESDTHPFVIAKTELSTAVQPNESTETTESLEVTWKPETYPETSEHFSGGEPDVFPTVPFHEEFESGTAKKGAESVTERDTEVGHQAHEHTEPVSLFPEESSGEIAIDQESQKIAFARATEVTFGEEVEKSTSVTYTPTIVPSSASAYVSEEEAVTLIGNPWPDDLLSTKESWVEATPRQVVELSGSSSIPITEGSGEAEEDEDTMFTMVTDLSQRNTTDTLITLDTSRIITESFFEVPATTIYPVSEQPSAKVVPTKFVSETDTSEWISSTTVEEKKRKEEEGTTGTASTFEVYSSTQRSDQLILPFELESPNVATSSDSGTRKSFMSLTTPTQSEREMTDSTPVFTETNTLENLGAQTTEHSSIHQPGVQEGLTTLPRSPASVFMEQGSGEAAADPETTTVSSFSLNVEYAIQAEKEVAGTLSPHVETTFSTEPTGLVLSTVMDRVVAENITQTSREIVISERLGEPNYGAEIRGFSTGFPLEEDFSGDFREYSTVSHPIAKEETVMMEGSGDAAFRDTQTSPSTVPTSVHISHISDSEGPSSTMVSTSAFPWEEFTSSAEGSGEQLVTVSSSVVPVLPSAVQKFSGTASSIIDEGLGEVGTVNEIDRRSTILPTAEVEGTKAPVEKEEVKVSGTVSTNFPQTIEPAKLWSRQEVNPVRQEIESETTSEEQIQEEKSFESPQNSPATEQTIFDSQTFTETELKTTDYSVLTTKKTYSDDKEMKEEDTSLVNMSTPDPDANGLESYTTLPEATEKSHFFLATALVTESIPAEHVVTDSPIKKEESTKHFPKGMRPTIQESDTELLFSGLGSGEEVLPTLPTESVNFTEVEQINNTLYPHTSQVESTSSDKIEDFNRMENVAKEVGPLVSQTDIFEGSGSVTSTTLIEILSDTGAEGPTVAPLPFSTDIGHPQNQTVRWAEEIQTSRPQTITEQDSNKNSSTAEINETTTSSTDFLARAYGFEMAKEFVTSAPKPSDLYYEPSGEGSGEVDIVDSFHTSATTQATRQESSTTFVSDGSLEKHPEVPSAKAVTADGFPTVSVMLPLHSEQNKSSPDPTSTLSNTVSYERSTDGSFQDRFREFEDSTLKPNRKKPTENIIIDLDKEDKDLILTITESTILEILPELTSDKNTIIDIDHTKPVYEDILGMQTDIDTEVPSEPHDSNDESNDDSTQVQEIYEAAVNLSLTEETFEGSADVLASYTQATHDESMTYEDRSQLDHMGFHFTTGIPAPSTETELDVLLPTATSLPIPRKSATVIPEIEGIKAEAKALDDMFESSTLSDGQAIADQSEIIPTLGQFERTQEEYEDKKHAGPSFQPEFSSGAEEALVDHTPYLSIATTHLMDQSVTEVPDVMEGSNPPYYTDTTLAVSTFAKLSSQTPSSPLTIYSGSEASGHTEIPQPSALPGIDVGSSVMSPQDSFKEIHVNIEATFKPSSEEYLHITEPPSLSPDTKLEPSEDDGKPELLEEMEASPTELIAVEGTEILQDFQNKTDGQVSGEAIKMFPTIKTPEAGTVITTADEIELEGATQWPHSTSASATYGVEAGVVPWLSPQTSERPTLSSSPEINPETQAALIRGQDSTIAASEQQVAARILDSNDQATVNPVEFNTEVATPPFSLLETSNETDFLIGINEESVEGTAIYLPGPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDQCELDFDECHSNPCRNGATCVDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAIPKITCMNPSAYQRTYSMKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Target ORF information:

RefSeq Version	NM_001164097.1
Organism	Homo sapiens(human)
Definition	Homo sapiens versican (VCAN), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164097.1

ORF Insert Sequence:

ATGTTCATAA ATATAAAGAG CATCTTATGG ATGTGTTCAA CCTTAATAGT AACCCATGCG 
CTACATAAAG TCAAAGTGGG AAAAAGCCCA CCGGTGAGGG GCTCCCTCTC TGGAAAAGTC 
AGCCTACCTT GTCATTTTTC AACGATGCCT ACTTTGCCAC CCAGTTACAA CACCAGTGAA 
TTTCTCCGCA TCAAATGGTC TAAGATTGAA GTGGACAAAA ATGGAAAAGA TTTGAAAGAG 
ACTACTGTCC TTGTGGCCCA AAATGGAAAT ATCAAGATTG GTCAGGACTA CAAAGGGAGA 
GTGTCTGTGC CCACACATCC CGAGGCTGTG GGCGATGCCT CCCTCACTGT GGTCAAGCTG 
CTGGCAAGTG ATGCGGGTCT TTACCGCTGT GACGTCATGT ACGGGATTGA AGACACACAA 
GACACGGTGT CACTGACTGT GGATGGGGTT GTGTTTCACT ACAGGGCGGC AACCAGCAGG 
TACACACTGA ATTTTGAGGC TGCTCAGAAG GCTTGTTTGG ACGTTGGGGC AGTCATAGCA 
ACTCCAGAGC AGCTCTTTGC TGCCTATGAA GATGGATTTG AGCAGTGTGA CGCAGGCTGG 
CTGGCTGATC AGACTGTCAG ATATCCCATC CGGGCTCCCA GAGTAGGCTG TTATGGAGAT 
AAGATGGGAA AGGCAGGAGT CAGGACTTAT GGATTCCGTT CTCCCCAGGA AACTTACGAT 
GTGTATTGTT ATGTGGATCA TCTGGATGGT GATGTGTTCC ACCTCACTGT CCCCAGTAAA 
TTCACCTTCG AGGAGGCTGC AAAAGAGTGT GAAAACCAGG ATGCCAGGCT GGCAACAGTG 
GGGGAACTCC AGGCGGCATG GAGGAACGGC TTTGACCAGT GCGATTACGG GTGGCTGTCG 
GATGCCAGCG TGCGCCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACAGGCT TCCCTCCCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACGTCGAATG AGTGATTTGA GTGTAATTGG TCATCCAATA 
GATTCAGAAT CTAAAGAAGA TGAACCTTGT AGTGAAGAAA CAGATCCAGT GCATGATCTA 
ATGGCTGAAA TTTTACCTGA ATTCCCTGAC ATAATTGAAA TAGACCTATA CCACAGTGAA 
GAAAATGAAG AAGAAGAAGA AGAGTGTGCA AATGCTACTG ATGTGACAAC CACCCCATCT 
GTGCAGTACA TAAATGGGAA GCATCTCGTT ACCACTGTGC CCAAGGACCC AGAAGCTGCA 
GAAGCTAGGC GTGGCCAGTT TGAAAGTGTT GCACCTTCTC AGAATTTCTC GGACAGCTCT 
GAAAGTGATA CTCATCCATT TGTAATAGCC AAAACGGAAT TGTCTACTGC TGTGCAACCT 
AATGAATCTA CAGAAACAAC TGAGTCTCTT GAAGTTACAT GGAAGCCTGA GACTTACCCT 
GAAACATCAG AACATTTTTC AGGTGGTGAG CCTGATGTTT TCCCCACAGT CCCATTCCAT 
GAGGAATTTG AAAGTGGAAC AGCCAAAAAA GGGGCAGAAT CAGTCACAGA GAGAGATACT 
GAAGTTGGTC ATCAGGCACA TGAACATACT GAACCTGTAT CTCTGTTTCC TGAAGAGTCT 
TCAGGAGAGA TTGCCATTGA CCAAGAATCT CAGAAAATAG CCTTTGCAAG GGCTACAGAA 
GTAACATTTG GTGAAGAGGT AGAAAAAAGT ACTTCTGTCA CATACACTCC CACTATAGTT 
CCAAGTTCTG CATCAGCATA TGTTTCAGAG GAAGAAGCAG TTACCCTAAT AGGAAATCCT 
TGGCCAGATG ACCTGTTGTC TACCAAAGAA AGCTGGGTAG AAGCAACTCC TAGACAAGTT 
GTAGAGCTCT CAGGGAGTTC TTCGATTCCA ATTACAGAAG GCTCTGGAGA AGCAGAAGAA 
GATGAAGATA CAATGTTCAC CATGGTAACT GATTTATCAC AGAGAAATAC TACTGATACA 
CTCATTACTT TAGACACTAG CAGGATAATC ACAGAAAGCT TTTTTGAGGT TCCTGCAACC 
ACCATTTATC CAGTTTCTGA ACAACCTTCT GCAAAAGTGG TGCCTACCAA GTTTGTAAGT 
GAAACAGACA CTTCTGAGTG GATTTCCAGT ACCACTGTTG AGGAAAAGAA AAGGAAGGAG 
GAGGAGGGAA CTACAGGTAC GGCTTCTACA TTTGAGGTAT ATTCATCTAC ACAGAGATCG 
GATCAATTAA TTTTACCCTT TGAATTAGAA AGTCCAAATG TAGCTACATC TAGTGATTCA 
GGTACCAGGA AAAGTTTTAT GTCCTTGACA ACACCAACAC AGTCTGAAAG GGAAATGACA 
GATTCTACTC CTGTCTTTAC AGAAACAAAT ACATTAGAAA ATTTGGGGGC ACAGACCACT 
GAGCACAGCA GTATCCATCA ACCTGGGGTT CAGGAAGGGC TGACCACTCT CCCACGTAGT 
CCTGCCTCTG TCTTTATGGA GCAGGGCTCT GGAGAAGCTG CTGCCGACCC AGAAACCACC 
ACTGTTTCTT CATTTTCATT AAACGTAGAG TATGCAATTC AAGCCGAAAA GGAAGTAGCT 
GGCACTTTGT CTCCGCATGT GGAAACTACA TTCTCCACTG AGCCAACAGG ACTGGTTTTG 
AGTACAGTAA TGGACAGAGT AGTTGCTGAA AATATAACCC AAACATCCAG GGAAATAGTG 
ATTTCAGAGC GATTAGGAGA ACCAAATTAT GGGGCAGAAA TAAGGGGCTT TTCCACAGGT 
TTTCCTTTGG AGGAAGATTT CAGTGGTGAC TTTAGAGAAT ACTCAACAGT GTCTCATCCC 
ATAGCAAAAG AAGAAACGGT AATGATGGAA GGCTCTGGAG ATGCAGCATT TAGGGACACC 
CAGACTTCAC CATCTACAGT ACCTACTTCA GTTCACATCA GTCACATATC TGACTCAGAA 
GGACCCAGTA GCACCATGGT CAGCACTTCA GCCTTCCCCT GGGAAGAGTT TACATCCTCA 
GCTGAGGGCT CAGGTGAGCA ACTGGTCACA GTCAGCAGCT CTGTTGTTCC AGTGCTTCCC 
AGTGCTGTGC AAAAGTTTTC TGGTACAGCT TCCTCCATTA TCGACGAAGG ATTGGGAGAA 
GTGGGTACTG TCAATGAAAT TGATAGAAGA TCCACCATTT TACCAACAGC AGAAGTGGAA 
GGTACGAAAG CTCCAGTAGA GAAGGAGGAA GTAAAGGTCA GTGGCACAGT TTCAACAAAC 
TTTCCCCAAA CTATAGAGCC AGCCAAATTA TGGTCTAGGC AAGAAGTCAA CCCTGTAAGA 
CAAGAAATTG AAAGTGAAAC AACATCAGAG GAACAAATTC AAGAAGAAAA GTCATTTGAA 
TCCCCTCAAA ACTCTCCTGC AACAGAACAA ACAATCTTTG ATTCACAGAC ATTTACTGAA 
ACTGAACTCA AAACCACAGA TTATTCTGTA CTAACAACAA AGAAAACTTA CAGTGATGAT 
AAAGAAATGA AGGAGGAAGA CACTTCTTTA GTTAACATGT CTACTCCAGA TCCAGATGCA 
AATGGCTTGG AATCTTACAC AACTCTCCCT GAAGCTACTG AAAAGTCACA TTTTTTCTTA 
GCTACTGCAT TAGTAACTGA ATCTATACCA GCTGAACATG TAGTCACAGA TTCACCAATC 
AAAAAGGAAG AAAGTACAAA ACATTTTCCG AAAGGCATGA GACCAACAAT TCAAGAGTCA 
GATACTGAGC TCTTATTCTC TGGACTGGGA TCAGGAGAAG AAGTTTTACC TACTCTACCA 
ACAGAGTCAG TGAATTTTAC TGAAGTGGAA CAAATCAATA ACACATTATA TCCCCACACT 
TCTCAAGTGG AAAGTACCTC AAGTGACAAA ATTGAAGACT TTAACAGAAT GGAAAATGTG 
GCAAAAGAAG TTGGACCACT CGTATCTCAA ACAGACATCT TTGAAGGTAG TGGGTCAGTA 
ACCAGCACAA CATTAATAGA AATTTTAAGT GACACTGGAG CAGAAGGACC CACGGTGGCA 
CCTCTCCCTT TCTCCACGGA CATCGGACAT CCTCAAAATC AGACTGTCAG GTGGGCAGAA 
GAAATCCAGA CTAGTAGACC ACAAACCATA ACTGAACAAG ACTCTAACAA GAATTCTTCA 
ACAGCAGAAA TTAACGAAAC AACAACCTCA TCTACTGATT TTCTGGCTAG AGCTTATGGT 
TTTGAAATGG CCAAAGAATT TGTTACATCA GCACCAAAAC CATCTGACTT GTATTATGAA 
CCTTCTGGAG AAGGATCTGG AGAAGTGGAT ATTGTTGATT CATTTCACAC TTCTGCAACT 
ACTCAGGCAA CCAGACAAGA AAGCAGCACC ACATTTGTTT CTGATGGGTC CCTGGAAAAA 
CATCCTGAGG TGCCAAGCGC TAAAGCTGTT ACTGCTGATG GATTCCCAAC AGTTTCAGTG 
ATGCTGCCTC TTCATTCAGA GCAGAACAAA AGCTCCCCTG ATCCAACTAG CACACTGTCA 
AATACAGTGT CATATGAGAG GTCCACAGAC GGTAGTTTCC AAGACCGTTT CAGGGAATTC 
GAGGATTCCA CCTTAAAACC TAACAGAAAA AAACCCACTG AAAATATTAT CATAGACCTG 
GACAAAGAGG ACAAGGATTT AATATTGACA ATTACAGAGA GTACCATCCT TGAAATTCTA 
CCTGAGCTGA CATCGGATAA AAATACTATC ATAGATATTG ATCATACTAA ACCTGTGTAT 
GAAGACATTC TTGGAATGCA AACAGATATA GATACAGAGG TACCATCAGA ACCACATGAC 
AGTAATGATG AAAGTAATGA TGACAGCACT CAAGTTCAAG AGATCTATGA GGCAGCTGTC 
AACCTTTCTT TAACTGAGGA AACATTTGAG GGCTCTGCTG ATGTTCTGGC TAGCTACACT 
CAGGCAACAC ATGATGAATC AATGACTTAT GAAGATAGAA GCCAACTAGA TCACATGGGC 
TTTCACTTCA CAACTGGGAT CCCTGCTCCT AGCACAGAAA CAGAATTAGA CGTTTTACTT 
CCCACGGCAA CATCCCTGCC AATTCCTCGT AAGTCTGCCA CAGTTATTCC AGAGATTGAA 
GGAATAAAAG CTGAAGCAAA AGCCCTGGAT GACATGTTTG AATCAAGCAC TTTGTCTGAT 
GGTCAAGCTA TTGCAGACCA AAGTGAAATA ATACCAACAT TGGGCCAATT TGAAAGGACT 
CAGGAGGAGT ATGAAGACAA AAAACATGCT GGTCCTTCTT TTCAGCCAGA ATTCTCTTCA 
GGAGCTGAGG AGGCATTAGT AGACCATACT CCCTATCTAA GTATTGCTAC TACCCACCTT 
ATGGATCAGA GTGTAACAGA GGTGCCTGAT GTGATGGAAG GATCCAATCC CCCATATTAC 
ACTGATACAA CATTAGCAGT TTCAACATTT GCGAAGTTGT CTTCTCAGAC ACCATCATCT 
CCCCTCACTA TCTACTCAGG CAGTGAAGCC TCTGGACACA CAGAGATCCC CCAGCCCAGT 
GCTCTGCCAG GAATAGACGT CGGCTCATCT GTAATGTCCC CACAGGATTC TTTTAAGGAA 
ATTCATGTAA ATATTGAAGC GACTTTCAAA CCATCAAGTG AGGAATACCT TCACATAACT 
GAGCCTCCCT CTTTATCTCC TGACACAAAA TTAGAACCTT CAGAAGATGA TGGTAAACCT 
GAGTTATTAG AAGAAATGGA AGCTTCTCCC ACAGAACTTA TTGCTGTGGA AGGAACTGAG 
ATTCTCCAAG ATTTCCAAAA CAAAACCGAT GGTCAAGTTT CTGGAGAAGC AATCAAGATG 
TTTCCCACCA TTAAAACACC TGAGGCTGGA ACTGTTATTA CAACTGCCGA TGAAATTGAA 
TTAGAAGGTG CTACACAGTG GCCACACTCT ACTTCTGCTT CTGCCACCTA TGGGGTCGAG 
GCAGGTGTGG TGCCTTGGCT AAGTCCACAG ACTTCTGAGA GGCCCACGCT TTCTTCTTCT 
CCAGAAATAA ACCCTGAAAC TCAAGCAGCT TTAATCAGAG GGCAGGATTC CACGATAGCA 
GCATCAGAAC AGCAAGTGGC AGCGAGAATT CTTGATTCCA ATGATCAGGC AACAGTAAAC 
CCTGTGGAAT TTAATACTGA GGTTGCAACA CCACCATTTT CCCTTCTGGA GACTTCTAAT 
GAAACAGATT TCCTGATTGG CATTAATGAA GAGTCAGTGG AAGGCACGGC AATCTATTTA 
CCAGGACCTG ATCGCTGCAA AATGAACCCG TGCCTTAACG GAGGCACCTG TTATCCTACT 
GAAACTTCCT ACGTATGCAC CTGTGTGCCA GGATACAGCG GAGACCAGTG TGAACTTGAT 
TTTGATGAAT GTCACTCTAA TCCCTGTCGT AATGGAGCCA CTTGTGTTGA TGGTTTTAAC 
ACATTCAGGT GCCTCTGCCT TCCAAGTTAT GTTGGTGCAC TTTGTGAGCA AGATACCGAG 
ACATGTGACT ATGGCTGGCA CAAATTCCAA GGGCAGTGCT ACAAATACTT TGCCCATCGA 
CGCACATGGG ATGCAGCTGA ACGGGAATGC CGTCTGCAGG GTGCCCATCT CACAAGCATC 
CTGTCTCACG AAGAACAAAT GTTTGTTAAT CGTGTGGGCC ATGATTATCA GTGGATAGGC 
CTCAATGACA AGATGTTTGA GCATGACTTC CGTTGGACTG ATGGCAGCAC ACTGCAATAC 
GAGAATTGGA GACCCAACCA GCCAGACAGC TTCTTTTCTG CTGGAGAAGA CTGTGTTGTA 
ATCATTTGGC ATGAGAATGG CCAGTGGAAT GATGTTCCCT GCAATTACCA TCTCACCTAT 
ACGTGCAAGA AAGGAACAGT CGCTTGCGGC CAGCCCCCTG TTGTAGAAAA TGCCAAGACC 
TTTGGAAAGA TGAAACCTCG TTATGAAATC AACTCCCTGA TTAGATACCA CTGCAAAGAT 
GGTTTCATTC AACGTCACCT TCCAACTATC CGGTGCTTAG GAAATGGAAG ATGGGCTATA 
CCTAAAATTA CCTGCATGAA CCCATCTGCA TACCAAAGGA CTTATTCTAT GAAATACTTT 
AAAAATTCCT CATCAGCAAA GGACAATTCA ATAAATACAT CCAAACATGA TCATCGTTGG 
AGCCGGAGGT GGCAGGAGTC GAGGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCAN ( NM_004385.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004376.2 Homo sapiens versican (VCAN), transcript variant 1, mRNA.

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CloneID	OHu22418
Clone ID Related Accession (Same CDS sequence)	NM_004385.5 , NM_004385.4
Accession Version	NM_004385.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	versican core protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA361670.1, U16306.1, AB209491.1, DT217515.1 and AC026696.5. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004385.4. Transcript Variant: This variant (1), also known as PG-M(V0), encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U16306.1, AB209491.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265077.8/ ENSP00000265077.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCATAA ATATAAAGAG CATCTTATGG ATGTGTTCAA CCTTAATAGT AACCCATGCG 
CTACATAAAG TCAAAGTGGG AAAAAGCCCA CCGGTGAGGG GCTCCCTCTC TGGAAAAGTC 
AGCCTACCTT GTCATTTTTC AACGATGCCT ACTTTGCCAC CCAGTTACAA CACCAGTGAA 
TTTCTCCGCA TCAAATGGTC TAAGATTGAA GTGGACAAAA ATGGAAAAGA TTTGAAAGAG 
ACTACTGTCC TTGTGGCCCA AAATGGAAAT ATCAAGATTG GTCAGGACTA CAAAGGGAGA 
GTGTCTGTGC CCACACATCC CGAGGCTGTG GGCGATGCCT CCCTCACTGT GGTCAAGCTG 
CTGGCAAGTG ATGCGGGTCT TTACCGCTGT GACGTCATGT ACGGGATTGA AGACACACAA 
GACACGGTGT CACTGACTGT GGATGGGGTT GTGTTTCACT ACAGGGCGGC AACCAGCAGG 
TACACACTGA ATTTTGAGGC TGCTCAGAAG GCTTGTTTGG ACGTTGGGGC AGTCATAGCA 
ACTCCAGAGC AGCTCTTTGC TGCCTATGAA GATGGATTTG AGCAGTGTGA CGCAGGCTGG 
CTGGCTGATC AGACTGTCAG ATATCCCATC CGGGCTCCCA GAGTAGGCTG TTATGGAGAT 
AAGATGGGAA AGGCAGGAGT CAGGACTTAT GGATTCCGTT CTCCCCAGGA AACTTACGAT 
GTGTATTGTT ATGTGGATCA TCTGGATGGT GATGTGTTCC ACCTCACTGT CCCCAGTAAA 
TTCACCTTCG AGGAGGCTGC AAAAGAGTGT GAAAACCAGG ATGCCAGGCT GGCAACAGTG 
GGGGAACTCC AGGCGGCATG GAGGAACGGC TTTGACCAGT GCGATTACGG GTGGCTGTCG 
GATGCCAGCG TGCGCCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACAGGCT TCCCTCCCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACCTAAAGAG GCTACAACCA TCGATTTGAG TATCCTCGCA 
GAAACTGCAT CACCCAGTTT ATCCAAAGAA CCACAAATGG TTTCTGATAG AACTACACCA 
ATCATCCCTT TAGTTGATGA ATTACCTGTC ATTCCAACAG AGTTCCCTCC CGTGGGAAAT 
ATTGTCAGTT TTGAACAGAA AGCCACAGTC CAACCTCAGG CTATCACAGA TAGTTTAGCC 
ACCAAATTAC CCACACCTAC TGGCAGTACC AAGAAGCCCT GGGATATGGA TGACTACTCA 
CCTTCTGCTT CAGGACCTCT TGGAAAGCTA GACATATCAG AAATTAAGGA AGAAGTGCTC 
CAGAGTACAA CTGGCGTCTC TCATTATGCT ACGGATTCAT GGGATGGTGT CGTGGAAGAT 
AAACAAACAC AAGAATCGGT TACACAGATT GAACAAATAG AAGTGGGTCC TTTGGTAACA 
TCTATGGAAA TCTTAAAGCA CATTCCTTCC AAGGAATTCC CTGTAACTGA AACACCATTG 
GTAACTGCAA GAATGATCCT GGAATCCAAA ACTGAAAAGA AAATGGTAAG CACTGTTTCT 
GAATTGGTAA CCACAGGTCA CTATGGATTC ACCTTGGGAG AAGAGGATGA TGAAGACAGA 
ACACTTACAG TTGGATCTGA TGAGAGCACC TTGATCTTTG ACCAAATTCC TGAAGTCATT 
ACGGTGTCAA AGACTTCAGA AGACACCATC CACACTCATT TAGAAGACTT GGAGTCAGTC 
TCAGCATCCA CAACTGTTTC CCCTTTAATT ATGCCTGATA ATAATGGATC ATCCATGGAT 
GACTGGGAAG AGAGACAAAC TAGTGGTAGG ATAACGGAAG AGTTTCTTGG CAAATATCTG 
TCTACTACAC CTTTTCCATC ACAGCATCGT ACAGAAATAG AATTGTTTCC TTATTCTGGT 
GATAAAATAT TAGTAGAGGG AATTTCCACA GTTATTTATC CTTCTCTACA AACAGAAATG 
ACACATAGAA GAGAAAGAAC AGAAACACTA ATACCAGAGA TGAGAACAGA TACTTATACA 
GATGAAATAC AAGAAGAGAT CACTAAAAGT CCATTTATGG GAAAAACAGA AGAAGAAGTC 
TTCTCTGGGA TGAAACTCTC TACATCTCTC TCAGAGCCAA TTCATGTTAC AGAGTCTTCT 
GTGGAAATGA CCAAGTCTTT TGATTTCCCA ACATTGATAA CAAAGTTAAG TGCAGAGCCA 
ACAGAAGTAA GAGATATGGA GGAAGACTTT ACAGCAACTC CAGGTACTAC AAAATATGAT 
GAAAATATTA CAACAGTGCT TTTGGCCCAT GGTACTTTAA GTGTTGAAGC AGCCACTGTA 
TCAAAATGGT CATGGGATGA AGATAATACA ACATCCAAGC CTTTAGAGTC TACAGAACCT 
TCAGCCTCTT CAAAATTGCC CCCTGCCTTA CTCACAACTG TGGGGATGAA TGGAAAGGAT 
AAAGACATCC CAAGTTTCAC TGAAGATGGA GCAGATGAAT TTACTCTTAT TCCAGATAGT 
ACTCAAAAGC AGTTAGAGGA GGTTACTGAT GAAGACATAG CAGCCCATGG AAAATTCACA 
ATTAGATTTC AGCCAACTAC ATCAACTGGT ATTGCAGAAA AGTCAACTTT GAGAGATTCT 
ACAACTGAAG AAAAAGTTCC ACCTATCACA AGCACTGAAG GCCAAGTTTA TGCAACCATG 
GAAGGAAGTG CTTTGGGTGA AGTAGAAGAT GTGGACCTCT CTAAGCCAGT ATCTACTGTT 
CCCCAATTTG CACACACTTC AGAGGTGGAA GGATTAGCAT TTGTTAGTTA TAGTAGCACC 
CAAGAGCCTA CTACTTATGT AGACTCTTCC CATACCATTC CTCTTTCTGT AATTCCCAAG 
ACAGACTGGG GAGTGTTAGT ACCTTCTGTT CCATCAGAAG ATGAAGTTCT AGGTGAACCC 
TCTCAAGACA TACTTGTCAT TGATCAGACT CGCCTTGAAG CGACTATTTC TCCAGAAACT 
ATGAGAACAA CAAAAATCAC AGAGGGAACA ACTCAGGAAG AATTCCCTTG GAAAGAACAG 
ACTGCAGAGA AACCAGTTCC TGCTCTCAGT TCTACAGCTT GGACTCCCAA GGAGGCAGTA 
ACACCACTGG ATGAACAAGA GGGCGATGGA TCAGCATATA CAGTCTCTGA AGATGAATTG 
TTGACAGGTT CTGAGAGGGT CCCAGTTTTA GAAACAACTC CAGTTGGAAA AATTGATCAC 
AGTGTGTCTT ATCCACCAGG TGCTGTAACT GAGCACAAAG TGAAAACAGA TGAAGTGGTA 
ACACTAACAC CACGCATTGG GCCAAAAGTA TCTTTAAGTC CAGGGCCTGA ACAAAAATAT 
GAAACAGAAG GTAGTAGTAC AACAGGATTT ACATCATCTT TGAGTCCTTT TAGTACCCAC 
ATTACCCAGC TTATGGAAGA AACCACTACT GAGAAAACAT CCCTAGAGGA TATTGATTTA 
GGCTCAGGAT TATTTGAAAA GCCCAAAGCC ACAGAACTCA TAGAATTTTC AACAATCAAA 
GTCACAGTTC CAAGTGATAT TACCACTGCC TTCAGTTCAG TAGACAGACT TCACACAACT 
TCAGCATTCA AGCCATCTTC CGCGATCACT AAGAAACCAC CTCTCATCGA CAGGGAACCT 
GGTGAAGAAA CAACCAGTGA CATGGTAATC ATTGGAGAAT CAACATCTCA TGTTCCTCCC 
ACTACCCTTG AAGATATTGT AGCCAAGGAA ACAGAAACCG ATATTGATAG AGAGTATTTC 
ACGACTTCAA GTCCTCCTGC TACACAGCCA ACAAGACCAC CCACTGTGGA AGACAAAGAG 
GCCTTTGGAC CTCAGGCGCT TTCTACGCCA CAGCCCCCAG CAAGCACAAA ATTTCACCCT 
GACATTAATG TTTATATTAT TGAGGTCAGA GAAAATAAGA CAGGTCGAAT GAGTGATTTG 
AGTGTAATTG GTCATCCAAT AGATTCAGAA TCTAAAGAAG ATGAACCTTG TAGTGAAGAA 
ACAGATCCAG TGCATGATCT AATGGCTGAA ATTTTACCTG AATTCCCTGA CATAATTGAA 
ATAGACCTAT ACCACAGTGA AGAAAATGAA GAAGAAGAAG AAGAGTGTGC AAATGCTACT 
GATGTGACAA CCACCCCATC TGTGCAGTAC ATAAATGGGA AGCATCTCGT TACCACTGTG 
CCCAAGGACC CAGAAGCTGC AGAAGCTAGG CGTGGCCAGT TTGAAAGTGT TGCACCTTCT 
CAGAATTTCT CGGACAGCTC TGAAAGTGAT ACTCATCCAT TTGTAATAGC CAAAACGGAA 
TTGTCTACTG CTGTGCAACC TAATGAATCT ACAGAAACAA CTGAGTCTCT TGAAGTTACA 
TGGAAGCCTG AGACTTACCC TGAAACATCA GAACATTTTT CAGGTGGTGA GCCTGATGTT 
TTCCCCACAG TCCCATTCCA TGAGGAATTT GAAAGTGGAA CAGCCAAAAA AGGGGCAGAA 
TCAGTCACAG AGAGAGATAC TGAAGTTGGT CATCAGGCAC ATGAACATAC TGAACCTGTA 
TCTCTGTTTC CTGAAGAGTC TTCAGGAGAG ATTGCCATTG ACCAAGAATC TCAGAAAATA 
GCCTTTGCAA GGGCTACAGA AGTAACATTT GGTGAAGAGG TAGAAAAAAG TACTTCTGTC 
ACATACACTC CCACTATAGT TCCAAGTTCT GCATCAGCAT ATGTTTCAGA GGAAGAAGCA 
GTTACCCTAA TAGGAAATCC TTGGCCAGAT GACCTGTTGT CTACCAAAGA AAGCTGGGTA 
GAAGCAACTC CTAGACAAGT TGTAGAGCTC TCAGGGAGTT CTTCGATTCC AATTACAGAA 
GGCTCTGGAG AAGCAGAAGA AGATGAAGAT ACAATGTTCA CCATGGTAAC TGATTTATCA 
CAGAGAAATA CTACTGATAC ACTCATTACT TTAGACACTA GCAGGATAAT CACAGAAAGC 
TTTTTTGAGG TTCCTGCAAC CACCATTTAT CCAGTTTCTG AACAACCTTC TGCAAAAGTG 
GTGCCTACCA AGTTTGTAAG TGAAACAGAC ACTTCTGAGT GGATTTCCAG TACCACTGTT 
GAGGAAAAGA AAAGGAAGGA GGAGGAGGGA ACTACAGGTA CGGCTTCTAC ATTTGAGGTA 
TATTCATCTA CACAGAGATC GGATCAATTA ATTTTACCCT TTGAATTAGA AAGTCCAAAT 
GTAGCTACAT CTAGTGATTC AGGTACCAGG AAAAGTTTTA TGTCCTTGAC AACACCAACA 
CAGTCTGAAA GGGAAATGAC AGATTCTACT CCTGTCTTTA CAGAAACAAA TACATTAGAA 
AATTTGGGGG CACAGACCAC TGAGCACAGC AGTATCCATC AACCTGGGGT TCAGGAAGGG 
CTGACCACTC TCCCACGTAG TCCTGCCTCT GTCTTTATGG AGCAGGGCTC TGGAGAAGCT 
GCTGCCGACC CAGAAACCAC CACTGTTTCT TCATTTTCAT TAAACGTAGA GTATGCAATT 
CAAGCCGAAA AGGAAGTAGC TGGCACTTTG TCTCCGCATG TGGAAACTAC ATTCTCCACT 
GAGCCAACAG GACTGGTTTT GAGTACAGTA ATGGACAGAG TAGTTGCTGA AAATATAACC 
CAAACATCCA GGGAAATAGT GATTTCAGAG CGATTAGGAG AACCAAATTA TGGGGCAGAA 
ATAAGGGGCT TTTCCACAGG TTTTCCTTTG GAGGAAGATT TCAGTGGTGA CTTTAGAGAA 
TACTCAACAG TGTCTCATCC CATAGCAAAA GAAGAAACGG TAATGATGGA AGGCTCTGGA 
GATGCAGCAT TTAGGGACAC CCAGACTTCA CCATCTACAG TACCTACTTC AGTTCACATC 
AGTCACATAT CTGACTCAGA AGGACCCAGT AGCACCATGG TCAGCACTTC AGCCTTCCCC 
TGGGAAGAGT TTACATCCTC AGCTGAGGGC TCAGGTGAGC AACTGGTCAC AGTCAGCAGC 
TCTGTTGTTC CAGTGCTTCC CAGTGCTGTG CAAAAGTTTT CTGGTACAGC TTCCTCCATT 
ATCGACGAAG GATTGGGAGA AGTGGGTACT GTCAATGAAA TTGATAGAAG ATCCACCATT 
TTACCAACAG CAGAAGTGGA AGGTACGAAA GCTCCAGTAG AGAAGGAGGA AGTAAAGGTC 
AGTGGCACAG TTTCAACAAA CTTTCCCCAA ACTATAGAGC CAGCCAAATT ATGGTCTAGG 
CAAGAAGTCA ACCCTGTAAG ACAAGAAATT GAAAGTGAAA CAACATCAGA GGAACAAATT 
CAAGAAGAAA AGTCATTTGA ATCCCCTCAA AACTCTCCTG CAACAGAACA AACAATCTTT 
GATTCACAGA CATTTACTGA AACTGAACTC AAAACCACAG ATTATTCTGT ACTAACAACA 
AAGAAAACTT ACAGTGATGA TAAAGAAATG AAGGAGGAAG ACACTTCTTT AGTTAACATG 
TCTACTCCAG ATCCAGATGC AAATGGCTTG GAATCTTACA CAACTCTCCC TGAAGCTACT 
GAAAAGTCAC ATTTTTTCTT AGCTACTGCA TTAGTAACTG AATCTATACC AGCTGAACAT 
GTAGTCACAG ATTCACCAAT CAAAAAGGAA GAAAGTACAA AACATTTTCC GAAAGGCATG 
AGACCAACAA TTCAAGAGTC AGATACTGAG CTCTTATTCT CTGGACTGGG ATCAGGAGAA 
GAAGTTTTAC CTACTCTACC AACAGAGTCA GTGAATTTTA CTGAAGTGGA ACAAATCAAT 
AACACATTAT ATCCCCACAC TTCTCAAGTG GAAAGTACCT CAAGTGACAA AATTGAAGAC 
TTTAACAGAA TGGAAAATGT GGCAAAAGAA GTTGGACCAC TCGTATCTCA AACAGACATC 
TTTGAAGGTA GTGGGTCAGT AACCAGCACA ACATTAATAG AAATTTTAAG TGACACTGGA 
GCAGAAGGAC CCACGGTGGC ACCTCTCCCT TTCTCCACGG ACATCGGACA TCCTCAAAAT 
CAGACTGTCA GGTGGGCAGA AGAAATCCAG ACTAGTAGAC CACAAACCAT AACTGAACAA 
GACTCTAACA AGAATTCTTC AACAGCAGAA ATTAACGAAA CAACAACCTC ATCTACTGAT 
TTTCTGGCTA GAGCTTATGG TTTTGAAATG GCCAAAGAAT TTGTTACATC AGCACCAAAA 
CCATCTGACT TGTATTATGA ACCTTCTGGA GAAGGATCTG GAGAAGTGGA TATTGTTGAT 
TCATTTCACA CTTCTGCAAC TACTCAGGCA ACCAGACAAG AAAGCAGCAC CACATTTGTT 
TCTGATGGGT CCCTGGAAAA ACATCCTGAG GTGCCAAGCG CTAAAGCTGT TACTGCTGAT 
GGATTCCCAA CAGTTTCAGT GATGCTGCCT CTTCATTCAG AGCAGAACAA AAGCTCCCCT 
GATCCAACTA GCACACTGTC AAATACAGTG TCATATGAGA GGTCCACAGA CGGTAGTTTC 
CAAGACCGTT TCAGGGAATT CGAGGATTCC ACCTTAAAAC CTAACAGAAA AAAACCCACT 
GAAAATATTA TCATAGACCT GGACAAAGAG GACAAGGATT TAATATTGAC AATTACAGAG 
AGTACCATCC TTGAAATTCT ACCTGAGCTG ACATCGGATA AAAATACTAT CATAGATATT 
GATCATACTA AACCTGTGTA TGAAGACATT CTTGGAATGC AAACAGATAT AGATACAGAG 
GTACCATCAG AACCACATGA CAGTAATGAT GAAAGTAATG ATGACAGCAC TCAAGTTCAA 
GAGATCTATG AGGCAGCTGT CAACCTTTCT TTAACTGAGG AAACATTTGA GGGCTCTGCT 
GATGTTCTGG CTAGCTACAC TCAGGCAACA CATGATGAAT CAATGACTTA TGAAGATAGA 
AGCCAACTAG ATCACATGGG CTTTCACTTC ACAACTGGGA TCCCTGCTCC TAGCACAGAA 
ACAGAATTAG ACGTTTTACT TCCCACGGCA ACATCCCTGC CAATTCCTCG TAAGTCTGCC 
ACAGTTATTC CAGAGATTGA AGGAATAAAA GCTGAAGCAA AAGCCCTGGA TGACATGTTT 
GAATCAAGCA CTTTGTCTGA TGGTCAAGCT ATTGCAGACC AAAGTGAAAT AATACCAACA 
TTGGGCCAAT TTGAAAGGAC TCAGGAGGAG TATGAAGACA AAAAACATGC TGGTCCTTCT 
TTTCAGCCAG AATTCTCTTC AGGAGCTGAG GAGGCATTAG TAGACCATAC TCCCTATCTA 
AGTATTGCTA CTACCCACCT TATGGATCAG AGTGTAACAG AGGTGCCTGA TGTGATGGAA 
GGATCCAATC CCCCATATTA CACTGATACA ACATTAGCAG TTTCAACATT TGCGAAGTTG 
TCTTCTCAGA CACCATCATC TCCCCTCACT ATCTACTCAG GCAGTGAAGC CTCTGGACAC 
ACAGAGATCC CCCAGCCCAG TGCTCTGCCA GGAATAGACG TCGGCTCATC TGTAATGTCC 
CCACAGGATT CTTTTAAGGA AATTCATGTA AATATTGAAG CGACTTTCAA ACCATCAAGT 
GAGGAATACC TTCACATAAC TGAGCCTCCC TCTTTATCTC CTGACACAAA ATTAGAACCT 
TCAGAAGATG ATGGTAAACC TGAGTTATTA GAAGAAATGG AAGCTTCTCC CACAGAACTT 
ATTGCTGTGG AAGGAACTGA GATTCTCCAA GATTTCCAAA ACAAAACCGA TGGTCAAGTT 
TCTGGAGAAG CAATCAAGAT GTTTCCCACC ATTAAAACAC CTGAGGCTGG AACTGTTATT 
ACAACTGCCG ATGAAATTGA ATTAGAAGGT GCTACACAGT GGCCACACTC TACTTCTGCT 
TCTGCCACCT ATGGGGTCGA GGCAGGTGTG GTGCCTTGGC TAAGTCCACA GACTTCTGAG 
AGGCCCACGC TTTCTTCTTC TCCAGAAATA AACCCTGAAA CTCAAGCAGC TTTAATCAGA 
GGGCAGGATT CCACGATAGC AGCATCAGAA CAGCAAGTGG CAGCGAGAAT TCTTGATTCC 
AATGATCAGG CAACAGTAAA CCCTGTGGAA TTTAATACTG AGGTTGCAAC ACCACCATTT 
TCCCTTCTGG AGACTTCTAA TGAAACAGAT TTCCTGATTG GCATTAATGA AGAGTCAGTG 
GAAGGCACGG CAATCTATTT ACCAGGACCT GATCGCTGCA AAATGAACCC GTGCCTTAAC 
GGAGGCACCT GTTATCCTAC TGAAACTTCC TACGTATGCA CCTGTGTGCC AGGATACAGC 
GGAGACCAGT GTGAACTTGA TTTTGATGAA TGTCACTCTA ATCCCTGTCG TAATGGAGCC 
ACTTGTGTTG ATGGTTTTAA CACATTCAGG TGCCTCTGCC TTCCAAGTTA TGTTGGTGCA 
CTTTGTGAGC AAGATACCGA GACATGTGAC TATGGCTGGC ACAAATTCCA AGGGCAGTGC 
TACAAATACT TTGCCCATCG ACGCACATGG GATGCAGCTG AACGGGAATG CCGTCTGCAG 
GGTGCCCATC TCACAAGCAT CCTGTCTCAC GAAGAACAAA TGTTTGTTAA TCGTGTGGGC 
CATGATTATC AGTGGATAGG CCTCAATGAC AAGATGTTTG AGCATGACTT CCGTTGGACT 
GATGGCAGCA CACTGCAATA CGAGAATTGG AGACCCAACC AGCCAGACAG CTTCTTTTCT 
GCTGGAGAAG ACTGTGTTGT AATCATTTGG CATGAGAATG GCCAGTGGAA TGATGTTCCC 
TGCAATTACC ATCTCACCTA TACGTGCAAG AAAGGAACAG TCGCTTGCGG CCAGCCCCCT 
GTTGTAGAAA ATGCCAAGAC CTTTGGAAAG ATGAAACCTC GTTATGAAAT CAACTCCCTG 
ATTAGATACC ACTGCAAAGA TGGTTTCATT CAACGTCACC TTCCAACTAT CCGGTGCTTA 
GGAAATGGAA GATGGGCTAT ACCTAAAATT ACCTGCATGA ACCCATCTGC ATACCAAAGG 
ACTTATTCTA TGAAATACTT TAAAAATTCC TCATCAGCAA AGGACAATTC AATAAATACA 
TCCAAACATG ATCATCGTTG GAGCCGGAGG TGGCAGGAGT CGAGGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004376.2
CDS	287..10477
Translation	MFINIKSILWMCSTLIVTHALHKVKVGKSPPVRGSLSGKVSLPCHFSTMPTLPPSYNTSEFLRIKWSKIEVDKNGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEAVGDASLTVVKLLASDAGLYRCDVMYGIEDTQDTVSLTVDGVVFHYRAATSRYTLNFEAAQKACLDVGAVIATPEQLFAAYEDGFEQCDAGWLADQTVRYPIRAPRVGCYGDKMGKAGVRTYGFRSPQETYDVYCYVDHLDGDVFHLTVPSKFTFEEAAKECENQDARLATVGELQAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPPPDSRFDAYCFKPKEATTIDLSILAETASPSLSKEPQMVSDRTTPIIPLVDELPVIPTEFPPVGNIVSFEQKATVQPQAITDSLATKLPTPTGSTKKPWDMDDYSPSASGPLGKLDISEIKEEVLQSTTGVSHYATDSWDGVVEDKQTQESVTQIEQIEVGPLVTSMEILKHIPSKEFPVTETPLVTARMILESKTEKKMVSTVSELVTTGHYGFTLGEEDDEDRTLTVGSDESTLIFDQIPEVITVSKTSEDTIHTHLEDLESVSASTTVSPLIMPDNNGSSMDDWEERQTSGRITEEFLGKYLSTTPFPSQHRTEIELFPYSGDKILVEGISTVIYPSLQTEMTHRRERTETLIPEMRTDTYTDEIQEEITKSPFMGKTEEEVFSGMKLSTSLSEPIHVTESSVEMTKSFDFPTLITKLSAEPTEVRDMEEDFTATPGTTKYDENITTVLLAHGTLSVEAATVSKWSWDEDNTTSKPLESTEPSASSKLPPALLTTVGMNGKDKDIPSFTEDGADEFTLIPDSTQKQLEEVTDEDIAAHGKFTIRFQPTTSTGIAEKSTLRDSTTEEKVPPITSTEGQVYATMEGSALGEVEDVDLSKPVSTVPQFAHTSEVEGLAFVSYSSTQEPTTYVDSSHTIPLSVIPKTDWGVLVPSVPSEDEVLGEPSQDILVIDQTRLEATISPETMRTTKITEGTTQEEFPWKEQTAEKPVPALSSTAWTPKEAVTPLDEQEGDGSAYTVSEDELLTGSERVPVLETTPVGKIDHSVSYPPGAVTEHKVKTDEVVTLTPRIGPKVSLSPGPEQKYETEGSSTTGFTSSLSPFSTHITQLMEETTTEKTSLEDIDLGSGLFEKPKATELIEFSTIKVTVPSDITTAFSSVDRLHTTSAFKPSSAITKKPPLIDREPGEETTSDMVIIGESTSHVPPTTLEDIVAKETETDIDREYFTTSSPPATQPTRPPTVEDKEAFGPQALSTPQPPASTKFHPDINVYIIEVRENKTGRMSDLSVIGHPIDSESKEDEPCSEETDPVHDLMAEILPEFPDIIEIDLYHSEENEEEEEECANATDVTTTPSVQYINGKHLVTTVPKDPEAAEARRGQFESVAPSQNFSDSSESDTHPFVIAKTELSTAVQPNESTETTESLEVTWKPETYPETSEHFSGGEPDVFPTVPFHEEFESGTAKKGAESVTERDTEVGHQAHEHTEPVSLFPEESSGEIAIDQESQKIAFARATEVTFGEEVEKSTSVTYTPTIVPSSASAYVSEEEAVTLIGNPWPDDLLSTKESWVEATPRQVVELSGSSSIPITEGSGEAEEDEDTMFTMVTDLSQRNTTDTLITLDTSRIITESFFEVPATTIYPVSEQPSAKVVPTKFVSETDTSEWISSTTVEEKKRKEEEGTTGTASTFEVYSSTQRSDQLILPFELESPNVATSSDSGTRKSFMSLTTPTQSEREMTDSTPVFTETNTLENLGAQTTEHSSIHQPGVQEGLTTLPRSPASVFMEQGSGEAAADPETTTVSSFSLNVEYAIQAEKEVAGTLSPHVETTFSTEPTGLVLSTVMDRVVAENITQTSREIVISERLGEPNYGAEIRGFSTGFPLEEDFSGDFREYSTVSHPIAKEETVMMEGSGDAAFRDTQTSPSTVPTSVHISHISDSEGPSSTMVSTSAFPWEEFTSSAEGSGEQLVTVSSSVVPVLPSAVQKFSGTASSIIDEGLGEVGTVNEIDRRSTILPTAEVEGTKAPVEKEEVKVSGTVSTNFPQTIEPAKLWSRQEVNPVRQEIESETTSEEQIQEEKSFESPQNSPATEQTIFDSQTFTETELKTTDYSVLTTKKTYSDDKEMKEEDTSLVNMSTPDPDANGLESYTTLPEATEKSHFFLATALVTESIPAEHVVTDSPIKKEESTKHFPKGMRPTIQESDTELLFSGLGSGEEVLPTLPTESVNFTEVEQINNTLYPHTSQVESTSSDKIEDFNRMENVAKEVGPLVSQTDIFEGSGSVTSTTLIEILSDTGAEGPTVAPLPFSTDIGHPQNQTVRWAEEIQTSRPQTITEQDSNKNSSTAEINETTTSSTDFLARAYGFEMAKEFVTSAPKPSDLYYEPSGEGSGEVDIVDSFHTSATTQATRQESSTTFVSDGSLEKHPEVPSAKAVTADGFPTVSVMLPLHSEQNKSSPDPTSTLSNTVSYERSTDGSFQDRFREFEDSTLKPNRKKPTENIIIDLDKEDKDLILTITESTILEILPELTSDKNTIIDIDHTKPVYEDILGMQTDIDTEVPSEPHDSNDESNDDSTQVQEIYEAAVNLSLTEETFEGSADVLASYTQATHDESMTYEDRSQLDHMGFHFTTGIPAPSTETELDVLLPTATSLPIPRKSATVIPEIEGIKAEAKALDDMFESSTLSDGQAIADQSEIIPTLGQFERTQEEYEDKKHAGPSFQPEFSSGAEEALVDHTPYLSIATTHLMDQSVTEVPDVMEGSNPPYYTDTTLAVSTFAKLSSQTPSSPLTIYSGSEASGHTEIPQPSALPGIDVGSSVMSPQDSFKEIHVNIEATFKPSSEEYLHITEPPSLSPDTKLEPSEDDGKPELLEEMEASPTELIAVEGTEILQDFQNKTDGQVSGEAIKMFPTIKTPEAGTVITTADEIELEGATQWPHSTSASATYGVEAGVVPWLSPQTSERPTLSSSPEINPETQAALIRGQDSTIAASEQQVAARILDSNDQATVNPVEFNTEVATPPFSLLETSNETDFLIGINEESVEGTAIYLPGPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDQCELDFDECHSNPCRNGATCVDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAIPKITCMNPSAYQRTYSMKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Target ORF information:

RefSeq Version	NM_004385.5
Organism	Homo sapiens(human)
Definition	Homo sapiens versican (VCAN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004385.5

ORF Insert Sequence:

ATGTTCATAA ATATAAAGAG CATCTTATGG ATGTGTTCAA CCTTAATAGT AACCCATGCG 
CTACATAAAG TCAAAGTGGG AAAAAGCCCA CCGGTGAGGG GCTCCCTCTC TGGAAAAGTC 
AGCCTACCTT GTCATTTTTC AACGATGCCT ACTTTGCCAC CCAGTTACAA CACCAGTGAA 
TTTCTCCGCA TCAAATGGTC TAAGATTGAA GTGGACAAAA ATGGAAAAGA TTTGAAAGAG 
ACTACTGTCC TTGTGGCCCA AAATGGAAAT ATCAAGATTG GTCAGGACTA CAAAGGGAGA 
GTGTCTGTGC CCACACATCC CGAGGCTGTG GGCGATGCCT CCCTCACTGT GGTCAAGCTG 
CTGGCAAGTG ATGCGGGTCT TTACCGCTGT GACGTCATGT ACGGGATTGA AGACACACAA 
GACACGGTGT CACTGACTGT GGATGGGGTT GTGTTTCACT ACAGGGCGGC AACCAGCAGG 
TACACACTGA ATTTTGAGGC TGCTCAGAAG GCTTGTTTGG ACGTTGGGGC AGTCATAGCA 
ACTCCAGAGC AGCTCTTTGC TGCCTATGAA GATGGATTTG AGCAGTGTGA CGCAGGCTGG 
CTGGCTGATC AGACTGTCAG ATATCCCATC CGGGCTCCCA GAGTAGGCTG TTATGGAGAT 
AAGATGGGAA AGGCAGGAGT CAGGACTTAT GGATTCCGTT CTCCCCAGGA AACTTACGAT 
GTGTATTGTT ATGTGGATCA TCTGGATGGT GATGTGTTCC ACCTCACTGT CCCCAGTAAA 
TTCACCTTCG AGGAGGCTGC AAAAGAGTGT GAAAACCAGG ATGCCAGGCT GGCAACAGTG 
GGGGAACTCC AGGCGGCATG GAGGAACGGC TTTGACCAGT GCGATTACGG GTGGCTGTCG 
GATGCCAGCG TGCGCCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACAGGCT TCCCTCCCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACCTAAAGAG GCTACAACCA TCGATTTGAG TATCCTCGCA 
GAAACTGCAT CACCCAGTTT ATCCAAAGAA CCACAAATGG TTTCTGATAG AACTACACCA 
ATCATCCCTT TAGTTGATGA ATTACCTGTC ATTCCAACAG AGTTCCCTCC CGTGGGAAAT 
ATTGTCAGTT TTGAACAGAA AGCCACAGTC CAACCTCAGG CTATCACAGA TAGTTTAGCC 
ACCAAATTAC CCACACCTAC TGGCAGTACC AAGAAGCCCT GGGATATGGA TGACTACTCA 
CCTTCTGCTT CAGGACCTCT TGGAAAGCTA GACATATCAG AAATTAAGGA AGAAGTGCTC 
CAGAGTACAA CTGGCGTCTC TCATTATGCT ACGGATTCAT GGGATGGTGT CGTGGAAGAT 
AAACAAACAC AAGAATCGGT TACACAGATT GAACAAATAG AAGTGGGTCC TTTGGTAACA 
TCTATGGAAA TCTTAAAGCA CATTCCTTCC AAGGAATTCC CTGTAACTGA AACACCATTG 
GTAACTGCAA GAATGATCCT GGAATCCAAA ACTGAAAAGA AAATGGTAAG CACTGTTTCT 
GAATTGGTAA CCACAGGTCA CTATGGATTC ACCTTGGGAG AAGAGGATGA TGAAGACAGA 
ACACTTACAG TTGGATCTGA TGAGAGCACC TTGATCTTTG ACCAAATTCC TGAAGTCATT 
ACGGTGTCAA AGACTTCAGA AGACACCATC CACACTCATT TAGAAGACTT GGAGTCAGTC 
TCAGCATCCA CAACTGTTTC CCCTTTAATT ATGCCTGATA ATAATGGATC ATCCATGGAT 
GACTGGGAAG AGAGACAAAC TAGTGGTAGG ATAACGGAAG AGTTTCTTGG CAAATATCTG 
TCTACTACAC CTTTTCCATC ACAGCATCGT ACAGAAATAG AATTGTTTCC TTATTCTGGT 
GATAAAATAT TAGTAGAGGG AATTTCCACA GTTATTTATC CTTCTCTACA AACAGAAATG 
ACACATAGAA GAGAAAGAAC AGAAACACTA ATACCAGAGA TGAGAACAGA TACTTATACA 
GATGAAATAC AAGAAGAGAT CACTAAAAGT CCATTTATGG GAAAAACAGA AGAAGAAGTC 
TTCTCTGGGA TGAAACTCTC TACATCTCTC TCAGAGCCAA TTCATGTTAC AGAGTCTTCT 
GTGGAAATGA CCAAGTCTTT TGATTTCCCA ACATTGATAA CAAAGTTAAG TGCAGAGCCA 
ACAGAAGTAA GAGATATGGA GGAAGACTTT ACAGCAACTC CAGGTACTAC AAAATATGAT 
GAAAATATTA CAACAGTGCT TTTGGCCCAT GGTACTTTAA GTGTTGAAGC AGCCACTGTA 
TCAAAATGGT CATGGGATGA AGATAATACA ACATCCAAGC CTTTAGAGTC TACAGAACCT 
TCAGCCTCTT CAAAATTGCC CCCTGCCTTA CTCACAACTG TGGGGATGAA TGGAAAGGAT 
AAAGACATCC CAAGTTTCAC TGAAGATGGA GCAGATGAAT TTACTCTTAT TCCAGATAGT 
ACTCAAAAGC AGTTAGAGGA GGTTACTGAT GAAGACATAG CAGCCCATGG AAAATTCACA 
ATTAGATTTC AGCCAACTAC ATCAACTGGT ATTGCAGAAA AGTCAACTTT GAGAGATTCT 
ACAACTGAAG AAAAAGTTCC ACCTATCACA AGCACTGAAG GCCAAGTTTA TGCAACCATG 
GAAGGAAGTG CTTTGGGTGA AGTAGAAGAT GTGGACCTCT CTAAGCCAGT ATCTACTGTT 
CCCCAATTTG CACACACTTC AGAGGTGGAA GGATTAGCAT TTGTTAGTTA TAGTAGCACC 
CAAGAGCCTA CTACTTATGT AGACTCTTCC CATACCATTC CTCTTTCTGT AATTCCCAAG 
ACAGACTGGG GAGTGTTAGT ACCTTCTGTT CCATCAGAAG ATGAAGTTCT AGGTGAACCC 
TCTCAAGACA TACTTGTCAT TGATCAGACT CGCCTTGAAG CGACTATTTC TCCAGAAACT 
ATGAGAACAA CAAAAATCAC AGAGGGAACA ACTCAGGAAG AATTCCCTTG GAAAGAACAG 
ACTGCAGAGA AACCAGTTCC TGCTCTCAGT TCTACAGCTT GGACTCCCAA GGAGGCAGTA 
ACACCACTGG ATGAACAAGA GGGCGATGGA TCAGCATATA CAGTCTCTGA AGATGAATTG 
TTGACAGGTT CTGAGAGGGT CCCAGTTTTA GAAACAACTC CAGTTGGAAA AATTGATCAC 
AGTGTGTCTT ATCCACCAGG TGCTGTAACT GAGCACAAAG TGAAAACAGA TGAAGTGGTA 
ACACTAACAC CACGCATTGG GCCAAAAGTA TCTTTAAGTC CAGGGCCTGA ACAAAAATAT 
GAAACAGAAG GTAGTAGTAC AACAGGATTT ACATCATCTT TGAGTCCTTT TAGTACCCAC 
ATTACCCAGC TTATGGAAGA AACCACTACT GAGAAAACAT CCCTAGAGGA TATTGATTTA 
GGCTCAGGAT TATTTGAAAA GCCCAAAGCC ACAGAACTCA TAGAATTTTC AACAATCAAA 
GTCACAGTTC CAAGTGATAT TACCACTGCC TTCAGTTCAG TAGACAGACT TCACACAACT 
TCAGCATTCA AGCCATCTTC CGCGATCACT AAGAAACCAC CTCTCATCGA CAGGGAACCT 
GGTGAAGAAA CAACCAGTGA CATGGTAATC ATTGGAGAAT CAACATCTCA TGTTCCTCCC 
ACTACCCTTG AAGATATTGT AGCCAAGGAA ACAGAAACCG ATATTGATAG AGAGTATTTC 
ACGACTTCAA GTCCTCCTGC TACACAGCCA ACAAGACCAC CCACTGTGGA AGACAAAGAG 
GCCTTTGGAC CTCAGGCGCT TTCTACGCCA CAGCCCCCAG CAAGCACAAA ATTTCACCCT 
GACATTAATG TTTATATTAT TGAGGTCAGA GAAAATAAGA CAGGTCGAAT GAGTGATTTG 
AGTGTAATTG GTCATCCAAT AGATTCAGAA TCTAAAGAAG ATGAACCTTG TAGTGAAGAA 
ACAGATCCAG TGCATGATCT AATGGCTGAA ATTTTACCTG AATTCCCTGA CATAATTGAA 
ATAGACCTAT ACCACAGTGA AGAAAATGAA GAAGAAGAAG AAGAGTGTGC AAATGCTACT 
GATGTGACAA CCACCCCATC TGTGCAGTAC ATAAATGGGA AGCATCTCGT TACCACTGTG 
CCCAAGGACC CAGAAGCTGC AGAAGCTAGG CGTGGCCAGT TTGAAAGTGT TGCACCTTCT 
CAGAATTTCT CGGACAGCTC TGAAAGTGAT ACTCATCCAT TTGTAATAGC CAAAACGGAA 
TTGTCTACTG CTGTGCAACC TAATGAATCT ACAGAAACAA CTGAGTCTCT TGAAGTTACA 
TGGAAGCCTG AGACTTACCC TGAAACATCA GAACATTTTT CAGGTGGTGA GCCTGATGTT 
TTCCCCACAG TCCCATTCCA TGAGGAATTT GAAAGTGGAA CAGCCAAAAA AGGGGCAGAA 
TCAGTCACAG AGAGAGATAC TGAAGTTGGT CATCAGGCAC ATGAACATAC TGAACCTGTA 
TCTCTGTTTC CTGAAGAGTC TTCAGGAGAG ATTGCCATTG ACCAAGAATC TCAGAAAATA 
GCCTTTGCAA GGGCTACAGA AGTAACATTT GGTGAAGAGG TAGAAAAAAG TACTTCTGTC 
ACATACACTC CCACTATAGT TCCAAGTTCT GCATCAGCAT ATGTTTCAGA GGAAGAAGCA 
GTTACCCTAA TAGGAAATCC TTGGCCAGAT GACCTGTTGT CTACCAAAGA AAGCTGGGTA 
GAAGCAACTC CTAGACAAGT TGTAGAGCTC TCAGGGAGTT CTTCGATTCC AATTACAGAA 
GGCTCTGGAG AAGCAGAAGA AGATGAAGAT ACAATGTTCA CCATGGTAAC TGATTTATCA 
CAGAGAAATA CTACTGATAC ACTCATTACT TTAGACACTA GCAGGATAAT CACAGAAAGC 
TTTTTTGAGG TTCCTGCAAC CACCATTTAT CCAGTTTCTG AACAACCTTC TGCAAAAGTG 
GTGCCTACCA AGTTTGTAAG TGAAACAGAC ACTTCTGAGT GGATTTCCAG TACCACTGTT 
GAGGAAAAGA AAAGGAAGGA GGAGGAGGGA ACTACAGGTA CGGCTTCTAC ATTTGAGGTA 
TATTCATCTA CACAGAGATC GGATCAATTA ATTTTACCCT TTGAATTAGA AAGTCCAAAT 
GTAGCTACAT CTAGTGATTC AGGTACCAGG AAAAGTTTTA TGTCCTTGAC AACACCAACA 
CAGTCTGAAA GGGAAATGAC AGATTCTACT CCTGTCTTTA CAGAAACAAA TACATTAGAA 
AATTTGGGGG CACAGACCAC TGAGCACAGC AGTATCCATC AACCTGGGGT TCAGGAAGGG 
CTGACCACTC TCCCACGTAG TCCTGCCTCT GTCTTTATGG AGCAGGGCTC TGGAGAAGCT 
GCTGCCGACC CAGAAACCAC CACTGTTTCT TCATTTTCAT TAAACGTAGA GTATGCAATT 
CAAGCCGAAA AGGAAGTAGC TGGCACTTTG TCTCCGCATG TGGAAACTAC ATTCTCCACT 
GAGCCAACAG GACTGGTTTT GAGTACAGTA ATGGACAGAG TAGTTGCTGA AAATATAACC 
CAAACATCCA GGGAAATAGT GATTTCAGAG CGATTAGGAG AACCAAATTA TGGGGCAGAA 
ATAAGGGGCT TTTCCACAGG TTTTCCTTTG GAGGAAGATT TCAGTGGTGA CTTTAGAGAA 
TACTCAACAG TGTCTCATCC CATAGCAAAA GAAGAAACGG TAATGATGGA AGGCTCTGGA 
GATGCAGCAT TTAGGGACAC CCAGACTTCA CCATCTACAG TACCTACTTC AGTTCACATC 
AGTCACATAT CTGACTCAGA AGGACCCAGT AGCACCATGG TCAGCACTTC AGCCTTCCCC 
TGGGAAGAGT TTACATCCTC AGCTGAGGGC TCAGGTGAGC AACTGGTCAC AGTCAGCAGC 
TCTGTTGTTC CAGTGCTTCC CAGTGCTGTG CAAAAGTTTT CTGGTACAGC TTCCTCCATT 
ATCGACGAAG GATTGGGAGA AGTGGGTACT GTCAATGAAA TTGATAGAAG ATCCACCATT 
TTACCAACAG CAGAAGTGGA AGGTACGAAA GCTCCAGTAG AGAAGGAGGA AGTAAAGGTC 
AGTGGCACAG TTTCAACAAA CTTTCCCCAA ACTATAGAGC CAGCCAAATT ATGGTCTAGG 
CAAGAAGTCA ACCCTGTAAG ACAAGAAATT GAAAGTGAAA CAACATCAGA GGAACAAATT 
CAAGAAGAAA AGTCATTTGA ATCCCCTCAA AACTCTCCTG CAACAGAACA AACAATCTTT 
GATTCACAGA CATTTACTGA AACTGAACTC AAAACCACAG ATTATTCTGT ACTAACAACA 
AAGAAAACTT ACAGTGATGA TAAAGAAATG AAGGAGGAAG ACACTTCTTT AGTTAACATG 
TCTACTCCAG ATCCAGATGC AAATGGCTTG GAATCTTACA CAACTCTCCC TGAAGCTACT 
GAAAAGTCAC ATTTTTTCTT AGCTACTGCA TTAGTAACTG AATCTATACC AGCTGAACAT 
GTAGTCACAG ATTCACCAAT CAAAAAGGAA GAAAGTACAA AACATTTTCC GAAAGGCATG 
AGACCAACAA TTCAAGAGTC AGATACTGAG CTCTTATTCT CTGGACTGGG ATCAGGAGAA 
GAAGTTTTAC CTACTCTACC AACAGAGTCA GTGAATTTTA CTGAAGTGGA ACAAATCAAT 
AACACATTAT ATCCCCACAC TTCTCAAGTG GAAAGTACCT CAAGTGACAA AATTGAAGAC 
TTTAACAGAA TGGAAAATGT GGCAAAAGAA GTTGGACCAC TCGTATCTCA AACAGACATC 
TTTGAAGGTA GTGGGTCAGT AACCAGCACA ACATTAATAG AAATTTTAAG TGACACTGGA 
GCAGAAGGAC CCACGGTGGC ACCTCTCCCT TTCTCCACGG ACATCGGACA TCCTCAAAAT 
CAGACTGTCA GGTGGGCAGA AGAAATCCAG ACTAGTAGAC CACAAACCAT AACTGAACAA 
GACTCTAACA AGAATTCTTC AACAGCAGAA ATTAACGAAA CAACAACCTC ATCTACTGAT 
TTTCTGGCTA GAGCTTATGG TTTTGAAATG GCCAAAGAAT TTGTTACATC AGCACCAAAA 
CCATCTGACT TGTATTATGA ACCTTCTGGA GAAGGATCTG GAGAAGTGGA TATTGTTGAT 
TCATTTCACA CTTCTGCAAC TACTCAGGCA ACCAGACAAG AAAGCAGCAC CACATTTGTT 
TCTGATGGGT CCCTGGAAAA ACATCCTGAG GTGCCAAGCG CTAAAGCTGT TACTGCTGAT 
GGATTCCCAA CAGTTTCAGT GATGCTGCCT CTTCATTCAG AGCAGAACAA AAGCTCCCCT 
GATCCAACTA GCACACTGTC AAATACAGTG TCATATGAGA GGTCCACAGA CGGTAGTTTC 
CAAGACCGTT TCAGGGAATT CGAGGATTCC ACCTTAAAAC CTAACAGAAA AAAACCCACT 
GAAAATATTA TCATAGACCT GGACAAAGAG GACAAGGATT TAATATTGAC AATTACAGAG 
AGTACCATCC TTGAAATTCT ACCTGAGCTG ACATCGGATA AAAATACTAT CATAGATATT 
GATCATACTA AACCTGTGTA TGAAGACATT CTTGGAATGC AAACAGATAT AGATACAGAG 
GTACCATCAG AACCACATGA CAGTAATGAT GAAAGTAATG ATGACAGCAC TCAAGTTCAA 
GAGATCTATG AGGCAGCTGT CAACCTTTCT TTAACTGAGG AAACATTTGA GGGCTCTGCT 
GATGTTCTGG CTAGCTACAC TCAGGCAACA CATGATGAAT CAATGACTTA TGAAGATAGA 
AGCCAACTAG ATCACATGGG CTTTCACTTC ACAACTGGGA TCCCTGCTCC TAGCACAGAA 
ACAGAATTAG ACGTTTTACT TCCCACGGCA ACATCCCTGC CAATTCCTCG TAAGTCTGCC 
ACAGTTATTC CAGAGATTGA AGGAATAAAA GCTGAAGCAA AAGCCCTGGA TGACATGTTT 
GAATCAAGCA CTTTGTCTGA TGGTCAAGCT ATTGCAGACC AAAGTGAAAT AATACCAACA 
TTGGGCCAAT TTGAAAGGAC TCAGGAGGAG TATGAAGACA AAAAACATGC TGGTCCTTCT 
TTTCAGCCAG AATTCTCTTC AGGAGCTGAG GAGGCATTAG TAGACCATAC TCCCTATCTA 
AGTATTGCTA CTACCCACCT TATGGATCAG AGTGTAACAG AGGTGCCTGA TGTGATGGAA 
GGATCCAATC CCCCATATTA CACTGATACA ACATTAGCAG TTTCAACATT TGCGAAGTTG 
TCTTCTCAGA CACCATCATC TCCCCTCACT ATCTACTCAG GCAGTGAAGC CTCTGGACAC 
ACAGAGATCC CCCAGCCCAG TGCTCTGCCA GGAATAGACG TCGGCTCATC TGTAATGTCC 
CCACAGGATT CTTTTAAGGA AATTCATGTA AATATTGAAG CGACTTTCAA ACCATCAAGT 
GAGGAATACC TTCACATAAC TGAGCCTCCC TCTTTATCTC CTGACACAAA ATTAGAACCT 
TCAGAAGATG ATGGTAAACC TGAGTTATTA GAAGAAATGG AAGCTTCTCC CACAGAACTT 
ATTGCTGTGG AAGGAACTGA GATTCTCCAA GATTTCCAAA ACAAAACCGA TGGTCAAGTT 
TCTGGAGAAG CAATCAAGAT GTTTCCCACC ATTAAAACAC CTGAGGCTGG AACTGTTATT 
ACAACTGCCG ATGAAATTGA ATTAGAAGGT GCTACACAGT GGCCACACTC TACTTCTGCT 
TCTGCCACCT ATGGGGTCGA GGCAGGTGTG GTGCCTTGGC TAAGTCCACA GACTTCTGAG 
AGGCCCACGC TTTCTTCTTC TCCAGAAATA AACCCTGAAA CTCAAGCAGC TTTAATCAGA 
GGGCAGGATT CCACGATAGC AGCATCAGAA CAGCAAGTGG CAGCGAGAAT TCTTGATTCC 
AATGATCAGG CAACAGTAAA CCCTGTGGAA TTTAATACTG AGGTTGCAAC ACCACCATTT 
TCCCTTCTGG AGACTTCTAA TGAAACAGAT TTCCTGATTG GCATTAATGA AGAGTCAGTG 
GAAGGCACGG CAATCTATTT ACCAGGACCT GATCGCTGCA AAATGAACCC GTGCCTTAAC 
GGAGGCACCT GTTATCCTAC TGAAACTTCC TACGTATGCA CCTGTGTGCC AGGATACAGC 
GGAGACCAGT GTGAACTTGA TTTTGATGAA TGTCACTCTA ATCCCTGTCG TAATGGAGCC 
ACTTGTGTTG ATGGTTTTAA CACATTCAGG TGCCTCTGCC TTCCAAGTTA TGTTGGTGCA 
CTTTGTGAGC AAGATACCGA GACATGTGAC TATGGCTGGC ACAAATTCCA AGGGCAGTGC 
TACAAATACT TTGCCCATCG ACGCACATGG GATGCAGCTG AACGGGAATG CCGTCTGCAG 
GGTGCCCATC TCACAAGCAT CCTGTCTCAC GAAGAACAAA TGTTTGTTAA TCGTGTGGGC 
CATGATTATC AGTGGATAGG CCTCAATGAC AAGATGTTTG AGCATGACTT CCGTTGGACT 
GATGGCAGCA CACTGCAATA CGAGAATTGG AGACCCAACC AGCCAGACAG CTTCTTTTCT 
GCTGGAGAAG ACTGTGTTGT AATCATTTGG CATGAGAATG GCCAGTGGAA TGATGTTCCC 
TGCAATTACC ATCTCACCTA TACGTGCAAG AAAGGAACAG TCGCTTGCGG CCAGCCCCCT 
GTTGTAGAAA ATGCCAAGAC CTTTGGAAAG ATGAAACCTC GTTATGAAAT CAACTCCCTG 
ATTAGATACC ACTGCAAAGA TGGTTTCATT CAACGTCACC TTCCAACTAT CCGGTGCTTA 
GGAAATGGAA GATGGGCTAT ACCTAAAATT ACCTGCATGA ACCCATCTGC ATACCAAAGG 
ACTTATTCTA TGAAATACTT TAAAAATTCC TCATCAGCAA AGGACAATTC AATAAATACA 
TCCAAACATG ATCATCGTTG GAGCCGGAGG TGGCAGGAGT CGAGGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCAN ( NM_004385.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004376.2 Homo sapiens versican (VCAN), transcript variant 1, mRNA.

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CloneID	OHu22418
Clone ID Related Accession (Same CDS sequence)	NM_004385.5 , NM_004385.4
Accession Version	NM_004385.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	versican core protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA361670.1, U16306.1, AB209491.1, DT217515.1 and AC026696.5. This sequence is a reference standard in the RefSeqGene project. On Aug 13, 2009 this sequence version replaced NM_004385.3. Transcript Variant: This variant (1), also known as PG-M(V0), encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U16306.1, AB209491.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGTTCATAA ATATAAAGAG CATCTTATGG ATGTGTTCAA CCTTAATAGT AACCCATGCG 
CTACATAAAG TCAAAGTGGG AAAAAGCCCA CCGGTGAGGG GCTCCCTCTC TGGAAAAGTC 
AGCCTACCTT GTCATTTTTC AACGATGCCT ACTTTGCCAC CCAGTTACAA CACCAGTGAA 
TTTCTCCGCA TCAAATGGTC TAAGATTGAA GTGGACAAAA ATGGAAAAGA TTTGAAAGAG 
ACTACTGTCC TTGTGGCCCA AAATGGAAAT ATCAAGATTG GTCAGGACTA CAAAGGGAGA 
GTGTCTGTGC CCACACATCC CGAGGCTGTG GGCGATGCCT CCCTCACTGT GGTCAAGCTG 
CTGGCAAGTG ATGCGGGTCT TTACCGCTGT GACGTCATGT ACGGGATTGA AGACACACAA 
GACACGGTGT CACTGACTGT GGATGGGGTT GTGTTTCACT ACAGGGCGGC AACCAGCAGG 
TACACACTGA ATTTTGAGGC TGCTCAGAAG GCTTGTTTGG ACGTTGGGGC AGTCATAGCA 
ACTCCAGAGC AGCTCTTTGC TGCCTATGAA GATGGATTTG AGCAGTGTGA CGCAGGCTGG 
CTGGCTGATC AGACTGTCAG ATATCCCATC CGGGCTCCCA GAGTAGGCTG TTATGGAGAT 
AAGATGGGAA AGGCAGGAGT CAGGACTTAT GGATTCCGTT CTCCCCAGGA AACTTACGAT 
GTGTATTGTT ATGTGGATCA TCTGGATGGT GATGTGTTCC ACCTCACTGT CCCCAGTAAA 
TTCACCTTCG AGGAGGCTGC AAAAGAGTGT GAAAACCAGG ATGCCAGGCT GGCAACAGTG 
GGGGAACTCC AGGCGGCATG GAGGAACGGC TTTGACCAGT GCGATTACGG GTGGCTGTCG 
GATGCCAGCG TGCGCCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACAGGCT TCCCTCCCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACCTAAAGAG GCTACAACCA TCGATTTGAG TATCCTCGCA 
GAAACTGCAT CACCCAGTTT ATCCAAAGAA CCACAAATGG TTTCTGATAG AACTACACCA 
ATCATCCCTT TAGTTGATGA ATTACCTGTC ATTCCAACAG AGTTCCCTCC CGTGGGAAAT 
ATTGTCAGTT TTGAACAGAA AGCCACAGTC CAACCTCAGG CTATCACAGA TAGTTTAGCC 
ACCAAATTAC CCACACCTAC TGGCAGTACC AAGAAGCCCT GGGATATGGA TGACTACTCA 
CCTTCTGCTT CAGGACCTCT TGGAAAGCTA GACATATCAG AAATTAAGGA AGAAGTGCTC 
CAGAGTACAA CTGGCGTCTC TCATTATGCT ACGGATTCAT GGGATGGTGT CGTGGAAGAT 
AAACAAACAC AAGAATCGGT TACACAGATT GAACAAATAG AAGTGGGTCC TTTGGTAACA 
TCTATGGAAA TCTTAAAGCA CATTCCTTCC AAGGAATTCC CTGTAACTGA AACACCATTG 
GTAACTGCAA GAATGATCCT GGAATCCAAA ACTGAAAAGA AAATGGTAAG CACTGTTTCT 
GAATTGGTAA CCACAGGTCA CTATGGATTC ACCTTGGGAG AAGAGGATGA TGAAGACAGA 
ACACTTACAG TTGGATCTGA TGAGAGCACC TTGATCTTTG ACCAAATTCC TGAAGTCATT 
ACGGTGTCAA AGACTTCAGA AGACACCATC CACACTCATT TAGAAGACTT GGAGTCAGTC 
TCAGCATCCA CAACTGTTTC CCCTTTAATT ATGCCTGATA ATAATGGATC ATCCATGGAT 
GACTGGGAAG AGAGACAAAC TAGTGGTAGG ATAACGGAAG AGTTTCTTGG CAAATATCTG 
TCTACTACAC CTTTTCCATC ACAGCATCGT ACAGAAATAG AATTGTTTCC TTATTCTGGT 
GATAAAATAT TAGTAGAGGG AATTTCCACA GTTATTTATC CTTCTCTACA AACAGAAATG 
ACACATAGAA GAGAAAGAAC AGAAACACTA ATACCAGAGA TGAGAACAGA TACTTATACA 
GATGAAATAC AAGAAGAGAT CACTAAAAGT CCATTTATGG GAAAAACAGA AGAAGAAGTC 
TTCTCTGGGA TGAAACTCTC TACATCTCTC TCAGAGCCAA TTCATGTTAC AGAGTCTTCT 
GTGGAAATGA CCAAGTCTTT TGATTTCCCA ACATTGATAA CAAAGTTAAG TGCAGAGCCA 
ACAGAAGTAA GAGATATGGA GGAAGACTTT ACAGCAACTC CAGGTACTAC AAAATATGAT 
GAAAATATTA CAACAGTGCT TTTGGCCCAT GGTACTTTAA GTGTTGAAGC AGCCACTGTA 
TCAAAATGGT CATGGGATGA AGATAATACA ACATCCAAGC CTTTAGAGTC TACAGAACCT 
TCAGCCTCTT CAAAATTGCC CCCTGCCTTA CTCACAACTG TGGGGATGAA TGGAAAGGAT 
AAAGACATCC CAAGTTTCAC TGAAGATGGA GCAGATGAAT TTACTCTTAT TCCAGATAGT 
ACTCAAAAGC AGTTAGAGGA GGTTACTGAT GAAGACATAG CAGCCCATGG AAAATTCACA 
ATTAGATTTC AGCCAACTAC ATCAACTGGT ATTGCAGAAA AGTCAACTTT GAGAGATTCT 
ACAACTGAAG AAAAAGTTCC ACCTATCACA AGCACTGAAG GCCAAGTTTA TGCAACCATG 
GAAGGAAGTG CTTTGGGTGA AGTAGAAGAT GTGGACCTCT CTAAGCCAGT ATCTACTGTT 
CCCCAATTTG CACACACTTC AGAGGTGGAA GGATTAGCAT TTGTTAGTTA TAGTAGCACC 
CAAGAGCCTA CTACTTATGT AGACTCTTCC CATACCATTC CTCTTTCTGT AATTCCCAAG 
ACAGACTGGG GAGTGTTAGT ACCTTCTGTT CCATCAGAAG ATGAAGTTCT AGGTGAACCC 
TCTCAAGACA TACTTGTCAT TGATCAGACT CGCCTTGAAG CGACTATTTC TCCAGAAACT 
ATGAGAACAA CAAAAATCAC AGAGGGAACA ACTCAGGAAG AATTCCCTTG GAAAGAACAG 
ACTGCAGAGA AACCAGTTCC TGCTCTCAGT TCTACAGCTT GGACTCCCAA GGAGGCAGTA 
ACACCACTGG ATGAACAAGA GGGCGATGGA TCAGCATATA CAGTCTCTGA AGATGAATTG 
TTGACAGGTT CTGAGAGGGT CCCAGTTTTA GAAACAACTC CAGTTGGAAA AATTGATCAC 
AGTGTGTCTT ATCCACCAGG TGCTGTAACT GAGCACAAAG TGAAAACAGA TGAAGTGGTA 
ACACTAACAC CACGCATTGG GCCAAAAGTA TCTTTAAGTC CAGGGCCTGA ACAAAAATAT 
GAAACAGAAG GTAGTAGTAC AACAGGATTT ACATCATCTT TGAGTCCTTT TAGTACCCAC 
ATTACCCAGC TTATGGAAGA AACCACTACT GAGAAAACAT CCCTAGAGGA TATTGATTTA 
GGCTCAGGAT TATTTGAAAA GCCCAAAGCC ACAGAACTCA TAGAATTTTC AACAATCAAA 
GTCACAGTTC CAAGTGATAT TACCACTGCC TTCAGTTCAG TAGACAGACT TCACACAACT 
TCAGCATTCA AGCCATCTTC CGCGATCACT AAGAAACCAC CTCTCATCGA CAGGGAACCT 
GGTGAAGAAA CAACCAGTGA CATGGTAATC ATTGGAGAAT CAACATCTCA TGTTCCTCCC 
ACTACCCTTG AAGATATTGT AGCCAAGGAA ACAGAAACCG ATATTGATAG AGAGTATTTC 
ACGACTTCAA GTCCTCCTGC TACACAGCCA ACAAGACCAC CCACTGTGGA AGACAAAGAG 
GCCTTTGGAC CTCAGGCGCT TTCTACGCCA CAGCCCCCAG CAAGCACAAA ATTTCACCCT 
GACATTAATG TTTATATTAT TGAGGTCAGA GAAAATAAGA CAGGTCGAAT GAGTGATTTG 
AGTGTAATTG GTCATCCAAT AGATTCAGAA TCTAAAGAAG ATGAACCTTG TAGTGAAGAA 
ACAGATCCAG TGCATGATCT AATGGCTGAA ATTTTACCTG AATTCCCTGA CATAATTGAA 
ATAGACCTAT ACCACAGTGA AGAAAATGAA GAAGAAGAAG AAGAGTGTGC AAATGCTACT 
GATGTGACAA CCACCCCATC TGTGCAGTAC ATAAATGGGA AGCATCTCGT TACCACTGTG 
CCCAAGGACC CAGAAGCTGC AGAAGCTAGG CGTGGCCAGT TTGAAAGTGT TGCACCTTCT 
CAGAATTTCT CGGACAGCTC TGAAAGTGAT ACTCATCCAT TTGTAATAGC CAAAACGGAA 
TTGTCTACTG CTGTGCAACC TAATGAATCT ACAGAAACAA CTGAGTCTCT TGAAGTTACA 
TGGAAGCCTG AGACTTACCC TGAAACATCA GAACATTTTT CAGGTGGTGA GCCTGATGTT 
TTCCCCACAG TCCCATTCCA TGAGGAATTT GAAAGTGGAA CAGCCAAAAA AGGGGCAGAA 
TCAGTCACAG AGAGAGATAC TGAAGTTGGT CATCAGGCAC ATGAACATAC TGAACCTGTA 
TCTCTGTTTC CTGAAGAGTC TTCAGGAGAG ATTGCCATTG ACCAAGAATC TCAGAAAATA 
GCCTTTGCAA GGGCTACAGA AGTAACATTT GGTGAAGAGG TAGAAAAAAG TACTTCTGTC 
ACATACACTC CCACTATAGT TCCAAGTTCT GCATCAGCAT ATGTTTCAGA GGAAGAAGCA 
GTTACCCTAA TAGGAAATCC TTGGCCAGAT GACCTGTTGT CTACCAAAGA AAGCTGGGTA 
GAAGCAACTC CTAGACAAGT TGTAGAGCTC TCAGGGAGTT CTTCGATTCC AATTACAGAA 
GGCTCTGGAG AAGCAGAAGA AGATGAAGAT ACAATGTTCA CCATGGTAAC TGATTTATCA 
CAGAGAAATA CTACTGATAC ACTCATTACT TTAGACACTA GCAGGATAAT CACAGAAAGC 
TTTTTTGAGG TTCCTGCAAC CACCATTTAT CCAGTTTCTG AACAACCTTC TGCAAAAGTG 
GTGCCTACCA AGTTTGTAAG TGAAACAGAC ACTTCTGAGT GGATTTCCAG TACCACTGTT 
GAGGAAAAGA AAAGGAAGGA GGAGGAGGGA ACTACAGGTA CGGCTTCTAC ATTTGAGGTA 
TATTCATCTA CACAGAGATC GGATCAATTA ATTTTACCCT TTGAATTAGA AAGTCCAAAT 
GTAGCTACAT CTAGTGATTC AGGTACCAGG AAAAGTTTTA TGTCCTTGAC AACACCAACA 
CAGTCTGAAA GGGAAATGAC AGATTCTACT CCTGTCTTTA CAGAAACAAA TACATTAGAA 
AATTTGGGGG CACAGACCAC TGAGCACAGC AGTATCCATC AACCTGGGGT TCAGGAAGGG 
CTGACCACTC TCCCACGTAG TCCTGCCTCT GTCTTTATGG AGCAGGGCTC TGGAGAAGCT 
GCTGCCGACC CAGAAACCAC CACTGTTTCT TCATTTTCAT TAAACGTAGA GTATGCAATT 
CAAGCCGAAA AGGAAGTAGC TGGCACTTTG TCTCCGCATG TGGAAACTAC ATTCTCCACT 
GAGCCAACAG GACTGGTTTT GAGTACAGTA ATGGACAGAG TAGTTGCTGA AAATATAACC 
CAAACATCCA GGGAAATAGT GATTTCAGAG CGATTAGGAG AACCAAATTA TGGGGCAGAA 
ATAAGGGGCT TTTCCACAGG TTTTCCTTTG GAGGAAGATT TCAGTGGTGA CTTTAGAGAA 
TACTCAACAG TGTCTCATCC CATAGCAAAA GAAGAAACGG TAATGATGGA AGGCTCTGGA 
GATGCAGCAT TTAGGGACAC CCAGACTTCA CCATCTACAG TACCTACTTC AGTTCACATC 
AGTCACATAT CTGACTCAGA AGGACCCAGT AGCACCATGG TCAGCACTTC AGCCTTCCCC 
TGGGAAGAGT TTACATCCTC AGCTGAGGGC TCAGGTGAGC AACTGGTCAC AGTCAGCAGC 
TCTGTTGTTC CAGTGCTTCC CAGTGCTGTG CAAAAGTTTT CTGGTACAGC TTCCTCCATT 
ATCGACGAAG GATTGGGAGA AGTGGGTACT GTCAATGAAA TTGATAGAAG ATCCACCATT 
TTACCAACAG CAGAAGTGGA AGGTACGAAA GCTCCAGTAG AGAAGGAGGA AGTAAAGGTC 
AGTGGCACAG TTTCAACAAA CTTTCCCCAA ACTATAGAGC CAGCCAAATT ATGGTCTAGG 
CAAGAAGTCA ACCCTGTAAG ACAAGAAATT GAAAGTGAAA CAACATCAGA GGAACAAATT 
CAAGAAGAAA AGTCATTTGA ATCCCCTCAA AACTCTCCTG CAACAGAACA AACAATCTTT 
GATTCACAGA CATTTACTGA AACTGAACTC AAAACCACAG ATTATTCTGT ACTAACAACA 
AAGAAAACTT ACAGTGATGA TAAAGAAATG AAGGAGGAAG ACACTTCTTT AGTTAACATG 
TCTACTCCAG ATCCAGATGC AAATGGCTTG GAATCTTACA CAACTCTCCC TGAAGCTACT 
GAAAAGTCAC ATTTTTTCTT AGCTACTGCA TTAGTAACTG AATCTATACC AGCTGAACAT 
GTAGTCACAG ATTCACCAAT CAAAAAGGAA GAAAGTACAA AACATTTTCC GAAAGGCATG 
AGACCAACAA TTCAAGAGTC AGATACTGAG CTCTTATTCT CTGGACTGGG ATCAGGAGAA 
GAAGTTTTAC CTACTCTACC AACAGAGTCA GTGAATTTTA CTGAAGTGGA ACAAATCAAT 
AACACATTAT ATCCCCACAC TTCTCAAGTG GAAAGTACCT CAAGTGACAA AATTGAAGAC 
TTTAACAGAA TGGAAAATGT GGCAAAAGAA GTTGGACCAC TCGTATCTCA AACAGACATC 
TTTGAAGGTA GTGGGTCAGT AACCAGCACA ACATTAATAG AAATTTTAAG TGACACTGGA 
GCAGAAGGAC CCACGGTGGC ACCTCTCCCT TTCTCCACGG ACATCGGACA TCCTCAAAAT 
CAGACTGTCA GGTGGGCAGA AGAAATCCAG ACTAGTAGAC CACAAACCAT AACTGAACAA 
GACTCTAACA AGAATTCTTC AACAGCAGAA ATTAACGAAA CAACAACCTC ATCTACTGAT 
TTTCTGGCTA GAGCTTATGG TTTTGAAATG GCCAAAGAAT TTGTTACATC AGCACCAAAA 
CCATCTGACT TGTATTATGA ACCTTCTGGA GAAGGATCTG GAGAAGTGGA TATTGTTGAT 
TCATTTCACA CTTCTGCAAC TACTCAGGCA ACCAGACAAG AAAGCAGCAC CACATTTGTT 
TCTGATGGGT CCCTGGAAAA ACATCCTGAG GTGCCAAGCG CTAAAGCTGT TACTGCTGAT 
GGATTCCCAA CAGTTTCAGT GATGCTGCCT CTTCATTCAG AGCAGAACAA AAGCTCCCCT 
GATCCAACTA GCACACTGTC AAATACAGTG TCATATGAGA GGTCCACAGA CGGTAGTTTC 
CAAGACCGTT TCAGGGAATT CGAGGATTCC ACCTTAAAAC CTAACAGAAA AAAACCCACT 
GAAAATATTA TCATAGACCT GGACAAAGAG GACAAGGATT TAATATTGAC AATTACAGAG 
AGTACCATCC TTGAAATTCT ACCTGAGCTG ACATCGGATA AAAATACTAT CATAGATATT 
GATCATACTA AACCTGTGTA TGAAGACATT CTTGGAATGC AAACAGATAT AGATACAGAG 
GTACCATCAG AACCACATGA CAGTAATGAT GAAAGTAATG ATGACAGCAC TCAAGTTCAA 
GAGATCTATG AGGCAGCTGT CAACCTTTCT TTAACTGAGG AAACATTTGA GGGCTCTGCT 
GATGTTCTGG CTAGCTACAC TCAGGCAACA CATGATGAAT CAATGACTTA TGAAGATAGA 
AGCCAACTAG ATCACATGGG CTTTCACTTC ACAACTGGGA TCCCTGCTCC TAGCACAGAA 
ACAGAATTAG ACGTTTTACT TCCCACGGCA ACATCCCTGC CAATTCCTCG TAAGTCTGCC 
ACAGTTATTC CAGAGATTGA AGGAATAAAA GCTGAAGCAA AAGCCCTGGA TGACATGTTT 
GAATCAAGCA CTTTGTCTGA TGGTCAAGCT ATTGCAGACC AAAGTGAAAT AATACCAACA 
TTGGGCCAAT TTGAAAGGAC TCAGGAGGAG TATGAAGACA AAAAACATGC TGGTCCTTCT 
TTTCAGCCAG AATTCTCTTC AGGAGCTGAG GAGGCATTAG TAGACCATAC TCCCTATCTA 
AGTATTGCTA CTACCCACCT TATGGATCAG AGTGTAACAG AGGTGCCTGA TGTGATGGAA 
GGATCCAATC CCCCATATTA CACTGATACA ACATTAGCAG TTTCAACATT TGCGAAGTTG 
TCTTCTCAGA CACCATCATC TCCCCTCACT ATCTACTCAG GCAGTGAAGC CTCTGGACAC 
ACAGAGATCC CCCAGCCCAG TGCTCTGCCA GGAATAGACG TCGGCTCATC TGTAATGTCC 
CCACAGGATT CTTTTAAGGA AATTCATGTA AATATTGAAG CGACTTTCAA ACCATCAAGT 
GAGGAATACC TTCACATAAC TGAGCCTCCC TCTTTATCTC CTGACACAAA ATTAGAACCT 
TCAGAAGATG ATGGTAAACC TGAGTTATTA GAAGAAATGG AAGCTTCTCC CACAGAACTT 
ATTGCTGTGG AAGGAACTGA GATTCTCCAA GATTTCCAAA ACAAAACCGA TGGTCAAGTT 
TCTGGAGAAG CAATCAAGAT GTTTCCCACC ATTAAAACAC CTGAGGCTGG AACTGTTATT 
ACAACTGCCG ATGAAATTGA ATTAGAAGGT GCTACACAGT GGCCACACTC TACTTCTGCT 
TCTGCCACCT ATGGGGTCGA GGCAGGTGTG GTGCCTTGGC TAAGTCCACA GACTTCTGAG 
AGGCCCACGC TTTCTTCTTC TCCAGAAATA AACCCTGAAA CTCAAGCAGC TTTAATCAGA 
GGGCAGGATT CCACGATAGC AGCATCAGAA CAGCAAGTGG CAGCGAGAAT TCTTGATTCC 
AATGATCAGG CAACAGTAAA CCCTGTGGAA TTTAATACTG AGGTTGCAAC ACCACCATTT 
TCCCTTCTGG AGACTTCTAA TGAAACAGAT TTCCTGATTG GCATTAATGA AGAGTCAGTG 
GAAGGCACGG CAATCTATTT ACCAGGACCT GATCGCTGCA AAATGAACCC GTGCCTTAAC 
GGAGGCACCT GTTATCCTAC TGAAACTTCC TACGTATGCA CCTGTGTGCC AGGATACAGC 
GGAGACCAGT GTGAACTTGA TTTTGATGAA TGTCACTCTA ATCCCTGTCG TAATGGAGCC 
ACTTGTGTTG ATGGTTTTAA CACATTCAGG TGCCTCTGCC TTCCAAGTTA TGTTGGTGCA 
CTTTGTGAGC AAGATACCGA GACATGTGAC TATGGCTGGC ACAAATTCCA AGGGCAGTGC 
TACAAATACT TTGCCCATCG ACGCACATGG GATGCAGCTG AACGGGAATG CCGTCTGCAG 
GGTGCCCATC TCACAAGCAT CCTGTCTCAC GAAGAACAAA TGTTTGTTAA TCGTGTGGGC 
CATGATTATC AGTGGATAGG CCTCAATGAC AAGATGTTTG AGCATGACTT CCGTTGGACT 
GATGGCAGCA CACTGCAATA CGAGAATTGG AGACCCAACC AGCCAGACAG CTTCTTTTCT 
GCTGGAGAAG ACTGTGTTGT AATCATTTGG CATGAGAATG GCCAGTGGAA TGATGTTCCC 
TGCAATTACC ATCTCACCTA TACGTGCAAG AAAGGAACAG TCGCTTGCGG CCAGCCCCCT 
GTTGTAGAAA ATGCCAAGAC CTTTGGAAAG ATGAAACCTC GTTATGAAAT CAACTCCCTG 
ATTAGATACC ACTGCAAAGA TGGTTTCATT CAACGTCACC TTCCAACTAT CCGGTGCTTA 
GGAAATGGAA GATGGGCTAT ACCTAAAATT ACCTGCATGA ACCCATCTGC ATACCAAAGG 
ACTTATTCTA TGAAATACTT TAAAAATTCC TCATCAGCAA AGGACAATTC AATAAATACA 
TCCAAACATG ATCATCGTTG GAGCCGGAGG TGGCAGGAGT CGAGGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004376.2
CDS	357..10547
Translation	MFINIKSILWMCSTLIVTHALHKVKVGKSPPVRGSLSGKVSLPCHFSTMPTLPPSYNTSEFLRIKWSKIEVDKNGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEAVGDASLTVVKLLASDAGLYRCDVMYGIEDTQDTVSLTVDGVVFHYRAATSRYTLNFEAAQKACLDVGAVIATPEQLFAAYEDGFEQCDAGWLADQTVRYPIRAPRVGCYGDKMGKAGVRTYGFRSPQETYDVYCYVDHLDGDVFHLTVPSKFTFEEAAKECENQDARLATVGELQAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPPPDSRFDAYCFKPKEATTIDLSILAETASPSLSKEPQMVSDRTTPIIPLVDELPVIPTEFPPVGNIVSFEQKATVQPQAITDSLATKLPTPTGSTKKPWDMDDYSPSASGPLGKLDISEIKEEVLQSTTGVSHYATDSWDGVVEDKQTQESVTQIEQIEVGPLVTSMEILKHIPSKEFPVTETPLVTARMILESKTEKKMVSTVSELVTTGHYGFTLGEEDDEDRTLTVGSDESTLIFDQIPEVITVSKTSEDTIHTHLEDLESVSASTTVSPLIMPDNNGSSMDDWEERQTSGRITEEFLGKYLSTTPFPSQHRTEIELFPYSGDKILVEGISTVIYPSLQTEMTHRRERTETLIPEMRTDTYTDEIQEEITKSPFMGKTEEEVFSGMKLSTSLSEPIHVTESSVEMTKSFDFPTLITKLSAEPTEVRDMEEDFTATPGTTKYDENITTVLLAHGTLSVEAATVSKWSWDEDNTTSKPLESTEPSASSKLPPALLTTVGMNGKDKDIPSFTEDGADEFTLIPDSTQKQLEEVTDEDIAAHGKFTIRFQPTTSTGIAEKSTLRDSTTEEKVPPITSTEGQVYATMEGSALGEVEDVDLSKPVSTVPQFAHTSEVEGLAFVSYSSTQEPTTYVDSSHTIPLSVIPKTDWGVLVPSVPSEDEVLGEPSQDILVIDQTRLEATISPETMRTTKITEGTTQEEFPWKEQTAEKPVPALSSTAWTPKEAVTPLDEQEGDGSAYTVSEDELLTGSERVPVLETTPVGKIDHSVSYPPGAVTEHKVKTDEVVTLTPRIGPKVSLSPGPEQKYETEGSSTTGFTSSLSPFSTHITQLMEETTTEKTSLEDIDLGSGLFEKPKATELIEFSTIKVTVPSDITTAFSSVDRLHTTSAFKPSSAITKKPPLIDREPGEETTSDMVIIGESTSHVPPTTLEDIVAKETETDIDREYFTTSSPPATQPTRPPTVEDKEAFGPQALSTPQPPASTKFHPDINVYIIEVRENKTGRMSDLSVIGHPIDSESKEDEPCSEETDPVHDLMAEILPEFPDIIEIDLYHSEENEEEEEECANATDVTTTPSVQYINGKHLVTTVPKDPEAAEARRGQFESVAPSQNFSDSSESDTHPFVIAKTELSTAVQPNESTETTESLEVTWKPETYPETSEHFSGGEPDVFPTVPFHEEFESGTAKKGAESVTERDTEVGHQAHEHTEPVSLFPEESSGEIAIDQESQKIAFARATEVTFGEEVEKSTSVTYTPTIVPSSASAYVSEEEAVTLIGNPWPDDLLSTKESWVEATPRQVVELSGSSSIPITEGSGEAEEDEDTMFTMVTDLSQRNTTDTLITLDTSRIITESFFEVPATTIYPVSEQPSAKVVPTKFVSETDTSEWISSTTVEEKKRKEEEGTTGTASTFEVYSSTQRSDQLILPFELESPNVATSSDSGTRKSFMSLTTPTQSEREMTDSTPVFTETNTLENLGAQTTEHSSIHQPGVQEGLTTLPRSPASVFMEQGSGEAAADPETTTVSSFSLNVEYAIQAEKEVAGTLSPHVETTFSTEPTGLVLSTVMDRVVAENITQTSREIVISERLGEPNYGAEIRGFSTGFPLEEDFSGDFREYSTVSHPIAKEETVMMEGSGDAAFRDTQTSPSTVPTSVHISHISDSEGPSSTMVSTSAFPWEEFTSSAEGSGEQLVTVSSSVVPVLPSAVQKFSGTASSIIDEGLGEVGTVNEIDRRSTILPTAEVEGTKAPVEKEEVKVSGTVSTNFPQTIEPAKLWSRQEVNPVRQEIESETTSEEQIQEEKSFESPQNSPATEQTIFDSQTFTETELKTTDYSVLTTKKTYSDDKEMKEEDTSLVNMSTPDPDANGLESYTTLPEATEKSHFFLATALVTESIPAEHVVTDSPIKKEESTKHFPKGMRPTIQESDTELLFSGLGSGEEVLPTLPTESVNFTEVEQINNTLYPHTSQVESTSSDKIEDFNRMENVAKEVGPLVSQTDIFEGSGSVTSTTLIEILSDTGAEGPTVAPLPFSTDIGHPQNQTVRWAEEIQTSRPQTITEQDSNKNSSTAEINETTTSSTDFLARAYGFEMAKEFVTSAPKPSDLYYEPSGEGSGEVDIVDSFHTSATTQATRQESSTTFVSDGSLEKHPEVPSAKAVTADGFPTVSVMLPLHSEQNKSSPDPTSTLSNTVSYERSTDGSFQDRFREFEDSTLKPNRKKPTENIIIDLDKEDKDLILTITESTILEILPELTSDKNTIIDIDHTKPVYEDILGMQTDIDTEVPSEPHDSNDESNDDSTQVQEIYEAAVNLSLTEETFEGSADVLASYTQATHDESMTYEDRSQLDHMGFHFTTGIPAPSTETELDVLLPTATSLPIPRKSATVIPEIEGIKAEAKALDDMFESSTLSDGQAIADQSEIIPTLGQFERTQEEYEDKKHAGPSFQPEFSSGAEEALVDHTPYLSIATTHLMDQSVTEVPDVMEGSNPPYYTDTTLAVSTFAKLSSQTPSSPLTIYSGSEASGHTEIPQPSALPGIDVGSSVMSPQDSFKEIHVNIEATFKPSSEEYLHITEPPSLSPDTKLEPSEDDGKPELLEEMEASPTELIAVEGTEILQDFQNKTDGQVSGEAIKMFPTIKTPEAGTVITTADEIELEGATQWPHSTSASATYGVEAGVVPWLSPQTSERPTLSSSPEINPETQAALIRGQDSTIAASEQQVAARILDSNDQATVNPVEFNTEVATPPFSLLETSNETDFLIGINEESVEGTAIYLPGPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDQCELDFDECHSNPCRNGATCVDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAIPKITCMNPSAYQRTYSMKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR

Target ORF information:

RefSeq Version	NM_004385.4
Organism	Homo sapiens(human)
Definition	Homo sapiens versican (VCAN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004385.4

ORF Insert Sequence:

ATGTTCATAA ATATAAAGAG CATCTTATGG ATGTGTTCAA CCTTAATAGT AACCCATGCG 
CTACATAAAG TCAAAGTGGG AAAAAGCCCA CCGGTGAGGG GCTCCCTCTC TGGAAAAGTC 
AGCCTACCTT GTCATTTTTC AACGATGCCT ACTTTGCCAC CCAGTTACAA CACCAGTGAA 
TTTCTCCGCA TCAAATGGTC TAAGATTGAA GTGGACAAAA ATGGAAAAGA TTTGAAAGAG 
ACTACTGTCC TTGTGGCCCA AAATGGAAAT ATCAAGATTG GTCAGGACTA CAAAGGGAGA 
GTGTCTGTGC CCACACATCC CGAGGCTGTG GGCGATGCCT CCCTCACTGT GGTCAAGCTG 
CTGGCAAGTG ATGCGGGTCT TTACCGCTGT GACGTCATGT ACGGGATTGA AGACACACAA 
GACACGGTGT CACTGACTGT GGATGGGGTT GTGTTTCACT ACAGGGCGGC AACCAGCAGG 
TACACACTGA ATTTTGAGGC TGCTCAGAAG GCTTGTTTGG ACGTTGGGGC AGTCATAGCA 
ACTCCAGAGC AGCTCTTTGC TGCCTATGAA GATGGATTTG AGCAGTGTGA CGCAGGCTGG 
CTGGCTGATC AGACTGTCAG ATATCCCATC CGGGCTCCCA GAGTAGGCTG TTATGGAGAT 
AAGATGGGAA AGGCAGGAGT CAGGACTTAT GGATTCCGTT CTCCCCAGGA AACTTACGAT 
GTGTATTGTT ATGTGGATCA TCTGGATGGT GATGTGTTCC ACCTCACTGT CCCCAGTAAA 
TTCACCTTCG AGGAGGCTGC AAAAGAGTGT GAAAACCAGG ATGCCAGGCT GGCAACAGTG 
GGGGAACTCC AGGCGGCATG GAGGAACGGC TTTGACCAGT GCGATTACGG GTGGCTGTCG 
GATGCCAGCG TGCGCCACCC TGTGACTGTG GCCAGGGCCC AGTGTGGAGG TGGTCTACTT 
GGGGTGAGAA CCCTGTATCG TTTTGAGAAC CAGACAGGCT TCCCTCCCCC TGATAGCAGA 
TTTGATGCCT ACTGCTTTAA ACCTAAAGAG GCTACAACCA TCGATTTGAG TATCCTCGCA 
GAAACTGCAT CACCCAGTTT ATCCAAAGAA CCACAAATGG TTTCTGATAG AACTACACCA 
ATCATCCCTT TAGTTGATGA ATTACCTGTC ATTCCAACAG AGTTCCCTCC CGTGGGAAAT 
ATTGTCAGTT TTGAACAGAA AGCCACAGTC CAACCTCAGG CTATCACAGA TAGTTTAGCC 
ACCAAATTAC CCACACCTAC TGGCAGTACC AAGAAGCCCT GGGATATGGA TGACTACTCA 
CCTTCTGCTT CAGGACCTCT TGGAAAGCTA GACATATCAG AAATTAAGGA AGAAGTGCTC 
CAGAGTACAA CTGGCGTCTC TCATTATGCT ACGGATTCAT GGGATGGTGT CGTGGAAGAT 
AAACAAACAC AAGAATCGGT TACACAGATT GAACAAATAG AAGTGGGTCC TTTGGTAACA 
TCTATGGAAA TCTTAAAGCA CATTCCTTCC AAGGAATTCC CTGTAACTGA AACACCATTG 
GTAACTGCAA GAATGATCCT GGAATCCAAA ACTGAAAAGA AAATGGTAAG CACTGTTTCT 
GAATTGGTAA CCACAGGTCA CTATGGATTC ACCTTGGGAG AAGAGGATGA TGAAGACAGA 
ACACTTACAG TTGGATCTGA TGAGAGCACC TTGATCTTTG ACCAAATTCC TGAAGTCATT 
ACGGTGTCAA AGACTTCAGA AGACACCATC CACACTCATT TAGAAGACTT GGAGTCAGTC 
TCAGCATCCA CAACTGTTTC CCCTTTAATT ATGCCTGATA ATAATGGATC ATCCATGGAT 
GACTGGGAAG AGAGACAAAC TAGTGGTAGG ATAACGGAAG AGTTTCTTGG CAAATATCTG 
TCTACTACAC CTTTTCCATC ACAGCATCGT ACAGAAATAG AATTGTTTCC TTATTCTGGT 
GATAAAATAT TAGTAGAGGG AATTTCCACA GTTATTTATC CTTCTCTACA AACAGAAATG 
ACACATAGAA GAGAAAGAAC AGAAACACTA ATACCAGAGA TGAGAACAGA TACTTATACA 
GATGAAATAC AAGAAGAGAT CACTAAAAGT CCATTTATGG GAAAAACAGA AGAAGAAGTC 
TTCTCTGGGA TGAAACTCTC TACATCTCTC TCAGAGCCAA TTCATGTTAC AGAGTCTTCT 
GTGGAAATGA CCAAGTCTTT TGATTTCCCA ACATTGATAA CAAAGTTAAG TGCAGAGCCA 
ACAGAAGTAA GAGATATGGA GGAAGACTTT ACAGCAACTC CAGGTACTAC AAAATATGAT 
GAAAATATTA CAACAGTGCT TTTGGCCCAT GGTACTTTAA GTGTTGAAGC AGCCACTGTA 
TCAAAATGGT CATGGGATGA AGATAATACA ACATCCAAGC CTTTAGAGTC TACAGAACCT 
TCAGCCTCTT CAAAATTGCC CCCTGCCTTA CTCACAACTG TGGGGATGAA TGGAAAGGAT 
AAAGACATCC CAAGTTTCAC TGAAGATGGA GCAGATGAAT TTACTCTTAT TCCAGATAGT 
ACTCAAAAGC AGTTAGAGGA GGTTACTGAT GAAGACATAG CAGCCCATGG AAAATTCACA 
ATTAGATTTC AGCCAACTAC ATCAACTGGT ATTGCAGAAA AGTCAACTTT GAGAGATTCT 
ACAACTGAAG AAAAAGTTCC ACCTATCACA AGCACTGAAG GCCAAGTTTA TGCAACCATG 
GAAGGAAGTG CTTTGGGTGA AGTAGAAGAT GTGGACCTCT CTAAGCCAGT ATCTACTGTT 
CCCCAATTTG CACACACTTC AGAGGTGGAA GGATTAGCAT TTGTTAGTTA TAGTAGCACC 
CAAGAGCCTA CTACTTATGT AGACTCTTCC CATACCATTC CTCTTTCTGT AATTCCCAAG 
ACAGACTGGG GAGTGTTAGT ACCTTCTGTT CCATCAGAAG ATGAAGTTCT AGGTGAACCC 
TCTCAAGACA TACTTGTCAT TGATCAGACT CGCCTTGAAG CGACTATTTC TCCAGAAACT 
ATGAGAACAA CAAAAATCAC AGAGGGAACA ACTCAGGAAG AATTCCCTTG GAAAGAACAG 
ACTGCAGAGA AACCAGTTCC TGCTCTCAGT TCTACAGCTT GGACTCCCAA GGAGGCAGTA 
ACACCACTGG ATGAACAAGA GGGCGATGGA TCAGCATATA CAGTCTCTGA AGATGAATTG 
TTGACAGGTT CTGAGAGGGT CCCAGTTTTA GAAACAACTC CAGTTGGAAA AATTGATCAC 
AGTGTGTCTT ATCCACCAGG TGCTGTAACT GAGCACAAAG TGAAAACAGA TGAAGTGGTA 
ACACTAACAC CACGCATTGG GCCAAAAGTA TCTTTAAGTC CAGGGCCTGA ACAAAAATAT 
GAAACAGAAG GTAGTAGTAC AACAGGATTT ACATCATCTT TGAGTCCTTT TAGTACCCAC 
ATTACCCAGC TTATGGAAGA AACCACTACT GAGAAAACAT CCCTAGAGGA TATTGATTTA 
GGCTCAGGAT TATTTGAAAA GCCCAAAGCC ACAGAACTCA TAGAATTTTC AACAATCAAA 
GTCACAGTTC CAAGTGATAT TACCACTGCC TTCAGTTCAG TAGACAGACT TCACACAACT 
TCAGCATTCA AGCCATCTTC CGCGATCACT AAGAAACCAC CTCTCATCGA CAGGGAACCT 
GGTGAAGAAA CAACCAGTGA CATGGTAATC ATTGGAGAAT CAACATCTCA TGTTCCTCCC 
ACTACCCTTG AAGATATTGT AGCCAAGGAA ACAGAAACCG ATATTGATAG AGAGTATTTC 
ACGACTTCAA GTCCTCCTGC TACACAGCCA ACAAGACCAC CCACTGTGGA AGACAAAGAG 
GCCTTTGGAC CTCAGGCGCT TTCTACGCCA CAGCCCCCAG CAAGCACAAA ATTTCACCCT 
GACATTAATG TTTATATTAT TGAGGTCAGA GAAAATAAGA CAGGTCGAAT GAGTGATTTG 
AGTGTAATTG GTCATCCAAT AGATTCAGAA TCTAAAGAAG ATGAACCTTG TAGTGAAGAA 
ACAGATCCAG TGCATGATCT AATGGCTGAA ATTTTACCTG AATTCCCTGA CATAATTGAA 
ATAGACCTAT ACCACAGTGA AGAAAATGAA GAAGAAGAAG AAGAGTGTGC AAATGCTACT 
GATGTGACAA CCACCCCATC TGTGCAGTAC ATAAATGGGA AGCATCTCGT TACCACTGTG 
CCCAAGGACC CAGAAGCTGC AGAAGCTAGG CGTGGCCAGT TTGAAAGTGT TGCACCTTCT 
CAGAATTTCT CGGACAGCTC TGAAAGTGAT ACTCATCCAT TTGTAATAGC CAAAACGGAA 
TTGTCTACTG CTGTGCAACC TAATGAATCT ACAGAAACAA CTGAGTCTCT TGAAGTTACA 
TGGAAGCCTG AGACTTACCC TGAAACATCA GAACATTTTT CAGGTGGTGA GCCTGATGTT 
TTCCCCACAG TCCCATTCCA TGAGGAATTT GAAAGTGGAA CAGCCAAAAA AGGGGCAGAA 
TCAGTCACAG AGAGAGATAC TGAAGTTGGT CATCAGGCAC ATGAACATAC TGAACCTGTA 
TCTCTGTTTC CTGAAGAGTC TTCAGGAGAG ATTGCCATTG ACCAAGAATC TCAGAAAATA 
GCCTTTGCAA GGGCTACAGA AGTAACATTT GGTGAAGAGG TAGAAAAAAG TACTTCTGTC 
ACATACACTC CCACTATAGT TCCAAGTTCT GCATCAGCAT ATGTTTCAGA GGAAGAAGCA 
GTTACCCTAA TAGGAAATCC TTGGCCAGAT GACCTGTTGT CTACCAAAGA AAGCTGGGTA 
GAAGCAACTC CTAGACAAGT TGTAGAGCTC TCAGGGAGTT CTTCGATTCC AATTACAGAA 
GGCTCTGGAG AAGCAGAAGA AGATGAAGAT ACAATGTTCA CCATGGTAAC TGATTTATCA 
CAGAGAAATA CTACTGATAC ACTCATTACT TTAGACACTA GCAGGATAAT CACAGAAAGC 
TTTTTTGAGG TTCCTGCAAC CACCATTTAT CCAGTTTCTG AACAACCTTC TGCAAAAGTG 
GTGCCTACCA AGTTTGTAAG TGAAACAGAC ACTTCTGAGT GGATTTCCAG TACCACTGTT 
GAGGAAAAGA AAAGGAAGGA GGAGGAGGGA ACTACAGGTA CGGCTTCTAC ATTTGAGGTA 
TATTCATCTA CACAGAGATC GGATCAATTA ATTTTACCCT TTGAATTAGA AAGTCCAAAT 
GTAGCTACAT CTAGTGATTC AGGTACCAGG AAAAGTTTTA TGTCCTTGAC AACACCAACA 
CAGTCTGAAA GGGAAATGAC AGATTCTACT CCTGTCTTTA CAGAAACAAA TACATTAGAA 
AATTTGGGGG CACAGACCAC TGAGCACAGC AGTATCCATC AACCTGGGGT TCAGGAAGGG 
CTGACCACTC TCCCACGTAG TCCTGCCTCT GTCTTTATGG AGCAGGGCTC TGGAGAAGCT 
GCTGCCGACC CAGAAACCAC CACTGTTTCT TCATTTTCAT TAAACGTAGA GTATGCAATT 
CAAGCCGAAA AGGAAGTAGC TGGCACTTTG TCTCCGCATG TGGAAACTAC ATTCTCCACT 
GAGCCAACAG GACTGGTTTT GAGTACAGTA ATGGACAGAG TAGTTGCTGA AAATATAACC 
CAAACATCCA GGGAAATAGT GATTTCAGAG CGATTAGGAG AACCAAATTA TGGGGCAGAA 
ATAAGGGGCT TTTCCACAGG TTTTCCTTTG GAGGAAGATT TCAGTGGTGA CTTTAGAGAA 
TACTCAACAG TGTCTCATCC CATAGCAAAA GAAGAAACGG TAATGATGGA AGGCTCTGGA 
GATGCAGCAT TTAGGGACAC CCAGACTTCA CCATCTACAG TACCTACTTC AGTTCACATC 
AGTCACATAT CTGACTCAGA AGGACCCAGT AGCACCATGG TCAGCACTTC AGCCTTCCCC 
TGGGAAGAGT TTACATCCTC AGCTGAGGGC TCAGGTGAGC AACTGGTCAC AGTCAGCAGC 
TCTGTTGTTC CAGTGCTTCC CAGTGCTGTG CAAAAGTTTT CTGGTACAGC TTCCTCCATT 
ATCGACGAAG GATTGGGAGA AGTGGGTACT GTCAATGAAA TTGATAGAAG ATCCACCATT 
TTACCAACAG CAGAAGTGGA AGGTACGAAA GCTCCAGTAG AGAAGGAGGA AGTAAAGGTC 
AGTGGCACAG TTTCAACAAA CTTTCCCCAA ACTATAGAGC CAGCCAAATT ATGGTCTAGG 
CAAGAAGTCA ACCCTGTAAG ACAAGAAATT GAAAGTGAAA CAACATCAGA GGAACAAATT 
CAAGAAGAAA AGTCATTTGA ATCCCCTCAA AACTCTCCTG CAACAGAACA AACAATCTTT 
GATTCACAGA CATTTACTGA AACTGAACTC AAAACCACAG ATTATTCTGT ACTAACAACA 
AAGAAAACTT ACAGTGATGA TAAAGAAATG AAGGAGGAAG ACACTTCTTT AGTTAACATG 
TCTACTCCAG ATCCAGATGC AAATGGCTTG GAATCTTACA CAACTCTCCC TGAAGCTACT 
GAAAAGTCAC ATTTTTTCTT AGCTACTGCA TTAGTAACTG AATCTATACC AGCTGAACAT 
GTAGTCACAG ATTCACCAAT CAAAAAGGAA GAAAGTACAA AACATTTTCC GAAAGGCATG 
AGACCAACAA TTCAAGAGTC AGATACTGAG CTCTTATTCT CTGGACTGGG ATCAGGAGAA 
GAAGTTTTAC CTACTCTACC AACAGAGTCA GTGAATTTTA CTGAAGTGGA ACAAATCAAT 
AACACATTAT ATCCCCACAC TTCTCAAGTG GAAAGTACCT CAAGTGACAA AATTGAAGAC 
TTTAACAGAA TGGAAAATGT GGCAAAAGAA GTTGGACCAC TCGTATCTCA AACAGACATC 
TTTGAAGGTA GTGGGTCAGT AACCAGCACA ACATTAATAG AAATTTTAAG TGACACTGGA 
GCAGAAGGAC CCACGGTGGC ACCTCTCCCT TTCTCCACGG ACATCGGACA TCCTCAAAAT 
CAGACTGTCA GGTGGGCAGA AGAAATCCAG ACTAGTAGAC CACAAACCAT AACTGAACAA 
GACTCTAACA AGAATTCTTC AACAGCAGAA ATTAACGAAA CAACAACCTC ATCTACTGAT 
TTTCTGGCTA GAGCTTATGG TTTTGAAATG GCCAAAGAAT TTGTTACATC AGCACCAAAA 
CCATCTGACT TGTATTATGA ACCTTCTGGA GAAGGATCTG GAGAAGTGGA TATTGTTGAT 
TCATTTCACA CTTCTGCAAC TACTCAGGCA ACCAGACAAG AAAGCAGCAC CACATTTGTT 
TCTGATGGGT CCCTGGAAAA ACATCCTGAG GTGCCAAGCG CTAAAGCTGT TACTGCTGAT 
GGATTCCCAA CAGTTTCAGT GATGCTGCCT CTTCATTCAG AGCAGAACAA AAGCTCCCCT 
GATCCAACTA GCACACTGTC AAATACAGTG TCATATGAGA GGTCCACAGA CGGTAGTTTC 
CAAGACCGTT TCAGGGAATT CGAGGATTCC ACCTTAAAAC CTAACAGAAA AAAACCCACT 
GAAAATATTA TCATAGACCT GGACAAAGAG GACAAGGATT TAATATTGAC AATTACAGAG 
AGTACCATCC TTGAAATTCT ACCTGAGCTG ACATCGGATA AAAATACTAT CATAGATATT 
GATCATACTA AACCTGTGTA TGAAGACATT CTTGGAATGC AAACAGATAT AGATACAGAG 
GTACCATCAG AACCACATGA CAGTAATGAT GAAAGTAATG ATGACAGCAC TCAAGTTCAA 
GAGATCTATG AGGCAGCTGT CAACCTTTCT TTAACTGAGG AAACATTTGA GGGCTCTGCT 
GATGTTCTGG CTAGCTACAC TCAGGCAACA CATGATGAAT CAATGACTTA TGAAGATAGA 
AGCCAACTAG ATCACATGGG CTTTCACTTC ACAACTGGGA TCCCTGCTCC TAGCACAGAA 
ACAGAATTAG ACGTTTTACT TCCCACGGCA ACATCCCTGC CAATTCCTCG TAAGTCTGCC 
ACAGTTATTC CAGAGATTGA AGGAATAAAA GCTGAAGCAA AAGCCCTGGA TGACATGTTT 
GAATCAAGCA CTTTGTCTGA TGGTCAAGCT ATTGCAGACC AAAGTGAAAT AATACCAACA 
TTGGGCCAAT TTGAAAGGAC TCAGGAGGAG TATGAAGACA AAAAACATGC TGGTCCTTCT 
TTTCAGCCAG AATTCTCTTC AGGAGCTGAG GAGGCATTAG TAGACCATAC TCCCTATCTA 
AGTATTGCTA CTACCCACCT TATGGATCAG AGTGTAACAG AGGTGCCTGA TGTGATGGAA 
GGATCCAATC CCCCATATTA CACTGATACA ACATTAGCAG TTTCAACATT TGCGAAGTTG 
TCTTCTCAGA CACCATCATC TCCCCTCACT ATCTACTCAG GCAGTGAAGC CTCTGGACAC 
ACAGAGATCC CCCAGCCCAG TGCTCTGCCA GGAATAGACG TCGGCTCATC TGTAATGTCC 
CCACAGGATT CTTTTAAGGA AATTCATGTA AATATTGAAG CGACTTTCAA ACCATCAAGT 
GAGGAATACC TTCACATAAC TGAGCCTCCC TCTTTATCTC CTGACACAAA ATTAGAACCT 
TCAGAAGATG ATGGTAAACC TGAGTTATTA GAAGAAATGG AAGCTTCTCC CACAGAACTT 
ATTGCTGTGG AAGGAACTGA GATTCTCCAA GATTTCCAAA ACAAAACCGA TGGTCAAGTT 
TCTGGAGAAG CAATCAAGAT GTTTCCCACC ATTAAAACAC CTGAGGCTGG AACTGTTATT 
ACAACTGCCG ATGAAATTGA ATTAGAAGGT GCTACACAGT GGCCACACTC TACTTCTGCT 
TCTGCCACCT ATGGGGTCGA GGCAGGTGTG GTGCCTTGGC TAAGTCCACA GACTTCTGAG 
AGGCCCACGC TTTCTTCTTC TCCAGAAATA AACCCTGAAA CTCAAGCAGC TTTAATCAGA 
GGGCAGGATT CCACGATAGC AGCATCAGAA CAGCAAGTGG CAGCGAGAAT TCTTGATTCC 
AATGATCAGG CAACAGTAAA CCCTGTGGAA TTTAATACTG AGGTTGCAAC ACCACCATTT 
TCCCTTCTGG AGACTTCTAA TGAAACAGAT TTCCTGATTG GCATTAATGA AGAGTCAGTG 
GAAGGCACGG CAATCTATTT ACCAGGACCT GATCGCTGCA AAATGAACCC GTGCCTTAAC 
GGAGGCACCT GTTATCCTAC TGAAACTTCC TACGTATGCA CCTGTGTGCC AGGATACAGC 
GGAGACCAGT GTGAACTTGA TTTTGATGAA TGTCACTCTA ATCCCTGTCG TAATGGAGCC 
ACTTGTGTTG ATGGTTTTAA CACATTCAGG TGCCTCTGCC TTCCAAGTTA TGTTGGTGCA 
CTTTGTGAGC AAGATACCGA GACATGTGAC TATGGCTGGC ACAAATTCCA AGGGCAGTGC 
TACAAATACT TTGCCCATCG ACGCACATGG GATGCAGCTG AACGGGAATG CCGTCTGCAG 
GGTGCCCATC TCACAAGCAT CCTGTCTCAC GAAGAACAAA TGTTTGTTAA TCGTGTGGGC 
CATGATTATC AGTGGATAGG CCTCAATGAC AAGATGTTTG AGCATGACTT CCGTTGGACT 
GATGGCAGCA CACTGCAATA CGAGAATTGG AGACCCAACC AGCCAGACAG CTTCTTTTCT 
GCTGGAGAAG ACTGTGTTGT AATCATTTGG CATGAGAATG GCCAGTGGAA TGATGTTCCC 
TGCAATTACC ATCTCACCTA TACGTGCAAG AAAGGAACAG TCGCTTGCGG CCAGCCCCCT 
GTTGTAGAAA ATGCCAAGAC CTTTGGAAAG ATGAAACCTC GTTATGAAAT CAACTCCCTG 
ATTAGATACC ACTGCAAAGA TGGTTTCATT CAACGTCACC TTCCAACTAT CCGGTGCTTA 
GGAAATGGAA GATGGGCTAT ACCTAAAATT ACCTGCATGA ACCCATCTGC ATACCAAAGG 
ACTTATTCTA TGAAATACTT TAAAAATTCC TCATCAGCAA AGGACAATTC AATAAATACA 
TCCAAACATG ATCATCGTTG GAGCCGGAGG TGGCAGGAGT CGAGGCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

WAGNER syndrome: anatomic, functional and genetic characterization of a Portuguese family.
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie256(1)163-171(2018 Jan)
Ara?jo JR,Tavares-Ferreira J,Estrela-Silva S,Rocha P,Brand?o E,Faria PA,Falc?o-Reis F,Rocha-Sousa A

MiR-135a-5p modulates biological functions of thyroid carcinoma cells via targeting VCAN 3'-UTR.
Cancer biomarkers : section A of Disease markers20(2)207-216(2017 Aug)
Zhao X,Sun Z,Li H,Jiang F,Zhou J,Zhang L

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VCAN cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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