RNF139 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF139
Entrez Gene ID	11236
Full Name	ring finger protein 139
Synonyms	HRCA1,RCA1,TRC8
General protein information	Preferred Names ring finger protein 139 Names E3 ubiquitin-protein ligase RNF139 RING-type E3 ubiquitin transferase RNF139 multiple membrane spanning receptor TRC8 patched related protein translocated in renal cancer translocation in renal carcinoma on chromosome 8 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q24.13
Summary	The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP. [provided by RefSeq, Jul 2008].
Disorder MIM:	603046
Disorder Html:	Renal cell carcinoma, 144700 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_007218.3	NP_009149.2	E3 ubiquitin-protein ligase RNF139
NM_007218.4	NP_009149.2	E3 ubiquitin-protein ligase RNF139
XM_017013002.1	XP_016868491.1	E3 ubiquitin-protein ligase RNF139 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	RNF139	NP_001026126.1
Xenopus tropicalis (tropical clawed frog)	rnf139	NP_001001242.1
Pan troglodytes (chimpanzee)	RNF139	XP_519946.2
Mus musculus (house mouse)	Rnf139	NP_780435.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003666	XP_313450.5
Homo sapiens (human)	RNF139	NP_009149.2
Bos taurus (cattle)	RNF139	XP_002692671.1
Rattus norvegicus (Norway rat)	Rnf139	NP_001121017.1
Drosophila melanogaster (fruit fly)	Trc8	NP_996303.1
	RNF139	XP_001102164.1
Canis lupus familiaris (dog)	RNF139	XP_851772.1
Danio rerio (zebrafish)	rnf139	NP_001116520.1

Gene Ontology
Bibliography

Related articles in PubMed

E3 ubiquitin ligase, RNF139, inhibits the progression of tongue cancer.
Wang L, Yin W, Shi C
BMC cancer17(1)452(2017 Jun)

Bag1 Co-chaperone Promotes TRC8 E3 Ligase-dependent Degradation of Misfolded Human Ether a Go-Go-related Gene (hERG) Potassium Channels.
Hantouche C, Williamson B, Valinsky WC, Solomon J, Shrier A, Young JC
The Journal of biological chemistry292(6)2287-2300(2017 02)

Identifying the ERAD ubiquitin E3 ligases for viral and cellular targeting of MHC class I.
van den Boomen DJ, Lehner PJ
Molecular immunology68(2 Pt A)106-11(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

interaction with Bag1 then shifts hERG degradation to the membrane-anchored E3 ligase TRC8 and its E2-conjugating enzyme Ube2g2, as determined by siRNA screening
Title: Bag1 Co-chaperone Promotes TRC8 E3 Ligase-dependent Degradation of Misfolded Human Ether a Go-Go-related Gene (hERG) Potassium Channels.

TRC8 and TMEM129 are endoplasmic reticulum-associated degradation ubiquitin E3 ligases for viral and cellular targeting of MHC class I. (Review)
Title: Identifying the ERAD ubiquitin E3 ligases for viral and cellular targeting of MHC class I.

TRC8 suppresses tumorigenesis through targeting heme oxygenase-1 for ubiquitination and degradation.
Title: TRC8 suppresses tumorigenesis through targeting heme oxygenase-1 for ubiquitination and degradation.

TRC8 function may provide a regulatory link between the lipid and protein biosynthetic pathways.
Title: The TRC8 ubiquitin ligase is sterol regulated and interacts with lipid and protein biosynthetic pathways.

Data show that the TRC8 E3 ligase is required for MHC I dislocation from the ER and identify a new complex associated with mammalian ERAD.
Title: The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.

The following RNF139 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF139 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18432	NM_007218.4 Latest version!	Homo sapiens ring finger protein 139 (RNF139), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu97718	XM_017013002.1 Latest version!	Homo sapiens ring finger protein 139 (RNF139), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu18432	NM_007218.3	Homo sapiens ring finger protein 139 (RNF139), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF139 ( NM_007218.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_009149.2 Homo sapiens ring finger protein 139 (RNF139), mRNA.

Price & Availability↑

CloneID	OHu18432
Clone ID Related Accession (Same CDS sequence)	NM_007218.3 , NM_007218.4
Accession Version	NM_007218.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF139
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI562637.1, CD171728.1, BC021571.1, BM804626.1, AA455970.1 and BM677460.1. This sequence is a reference standard in the RefSeqGene project. On Oct 30, 2003 this sequence version replaced NM_007218.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA381454.1, AF064801.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG TGGGGCCCCC GCAGCAGCAG GTGCGGATGG CCCATCAGCA GGTCTGGGCG 
GCGCTCGAAG TGGCGCTCCG GGTGCCCTGC CTTTACATCA TCGACGCCAT CTTCAACTCC 
TACCCGGATT CCAGCCAAAG CCGGTTCTGC ATCGTGCTCC AGATCTTCCT CCGGCTCTTT 
GGTGTATTTG CATCCAGTAT TGTTCTGATC TTGTCACAAC GATCACTTTT CAAGTTTTAC 
ACGTACAGCT CAGCCTTTCT GTTAGCTGCA ACTTCAGTGT TGGTGAATTA TTATGCTTCT 
TTGCACATTG ACTTCTATGG TGCCTACAAC ACGTCAGCTT TTGGAATTGA GCTGCTTCCT 
CGAAAAGGTC CCTCGCTGTG GATGGCACTT ATCGTTCTAC AGCTAACATT TGGAATTGGA 
TACGTTACAC TACTCCAGAT TCATTCCATC TATTCACAAT TAATTATTTT GGATCTCTTG 
GTTCCTGTAA TAGGCTTAAT CACAGAGCTA CCATTACACA TCAGAGAGAC TTTACTGTTT 
ACTTCTTCCT TGATTCTCAC ATTAAATACA GTGTTTGTCC TGGCAGTGAA ACTGAAGTGG 
TTTTATTATT CCACACGATA TGTTTATCTT TTGGTGAGGC ACATGTATCG AATTTACGGA 
TTACAGTTAT TGATGGAGGA CACATGGAAG AGGATTCGTT TCCCAGACAT ACTACGAGTC 
TTTTGGCTAA CAAGAGTTAC AGCTCAGGCT ACAGTGTTAA TGTACATCTT AAGGATGGCA 
AATGAAACTG ATTCCTTCTT TATTTCTTGG GATGATTTTT GGGACCTCAT TTGCAATCTT 
ATAATTAGTG GGTGCGATTC TACACTAACT GTACTGGGCA TGAGTGCTGT AATTTCCTCA 
GTAGCCCATT ATTTGGGGCT TGGAATATTG GCCTTTATTG GATCAACTGA GGAAGATGAC 
AGGCGTCTTG GCTTTGTTGC ACCTGTTTTA TTTTTTATTT TGGCTCTTCA GACTGGGTTA 
AGTGGGCTAA GACCAGAAGA GAGACTTATT CGCTTAAGTA GAAACATGTG CCTTTTATTA 
ACTGCAGTCC TGCATTTTAT CCATGGAATG ACAGACCCTG TATTAATGTC TCTCAGTGCC 
TCTCATGTGT CATCTTTTCG TAGACATTTT CCTGTGCTGT TTGTCTCTGC TTGCCTGTTT 
ATTCTTCCTG TCTTACTCAG TTATGTTCTT TGGCATCACT ATGCACTAAA TACATGGTTG 
TTTGCAGTTA CAGCATTTTG TGTGGAACTG TGCTTAAAAG TAATTGTTTC TCTCACTGTT 
TATACGTTAT TCATGATTGA TGGCTACTAT AATGTCCTCT GGGAAAAGCT TGACGATTAT 
GTCTACTACG TTCGTTCAAC AGGCAGTATT ATTGAATTTA TATTTGGAGT TGTAATGTTT 
GGAAATGGGG CTTACACTAT GATGTTTGAG TCGGGAAGTA AAATTCGGGC TTTTATGATG 
TGCCTACATG CATATTTTAA CATCTACTTA CAAGCCAAAA ATGGCTGGAA GACATTTATG 
AATCGTAGGA CTGCTGTGAA GAAAATTAAT TCACTTCCTG AAATAAAAGG GAGCCGCTTA 
CAAGAAATAA ATGATGTATG TGCAATCTGC TATCATGAGT TTACAACATC TGCTCGTATT 
ACACCGTGTA ATCATTATTT CCATGCACTT TGCCTTCGGA AATGGCTGTA CATTCAAGAT 
ACTTGTCCAA TGTGCCATCA GAAAGTATAC ATCGAAGATG ATATCAAGGA TAATTCAAAT 
GTATCTAACA ACAATGGATT TATTCCACCC AATGAAACTC CAGAGGAAGC TGTAAGAGAA 
GCTGCTGCTG AATCTGACAG GGAATTGAAC GAAGATGACA GTACAGATTG TGATGATGAT 
GTTCAAAGAG AAAGAAATGG AGTGATTCAG CACACAGGCG CAGCAGCTGA AGAATTTAAT 
GATGATACTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009149.2
CDS	344..2338
Translation	MAAVGPPQQQVRMAHQQVWAALEVALRVPCLYIIDAIFNSYPDSSQSRFCIVLQIFLRLFGVFASSIVLILSQRSLFKFYTYSSAFLLAATSVLVNYYASLHIDFYGAYNTSAFGIELLPRKGPSLWMALIVLQLTFGIGYVTLLQIHSIYSQLIILDLLVPVIGLITELPLHIRETLLFTSSLILTLNTVFVLAVKLKWFYYSTRYVYLLVRHMYRIYGLQLLMEDTWKRIRFPDILRVFWLTRVTAQATVLMYILRMANETDSFFISWDDFWDLICNLIISGCDSTLTVLGMSAVISSVAHYLGLGILAFIGSTEEDDRRLGFVAPVLFFILALQTGLSGLRPEERLIRLSRNMCLLLTAVLHFIHGMTDPVLMSLSASHVSSFRRHFPVLFVSACLFILPVLLSYVLWHHYALNTWLFAVTAFCVELCLKVIVSLTVYTLFMIDGYYNVLWEKLDDYVYYVRSTGSIIEFIFGVVMFGNGAYTMMFESGSKIRAFMMCLHAYFNIYLQAKNGWKTFMNRRTAVKKINSLPEIKGSRLQEINDVCAICYHEFTTSARITPCNHYFHALCLRKWLYIQDTCPMCHQKVYIEDDIKDNSNVSNNNGFIPPNETPEEAVREAAAESDRELNEDDSTDCDDDVQRERNGVIQHTGAAAEEFNDDTD

Target ORF information:

RefSeq Version	NM_007218.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 139 (RNF139), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007218.3

ORF Insert Sequence:

ATGGCGGCCG TGGGGCCCCC GCAGCAGCAG GTGCGGATGG CCCATCAGCA GGTCTGGGCG 
GCGCTCGAAG TGGCGCTCCG GGTGCCCTGC CTTTACATCA TCGACGCCAT CTTCAACTCC 
TACCCGGATT CCAGCCAAAG CCGGTTCTGC ATCGTGCTCC AGATCTTCCT CCGGCTCTTT 
GGTGTATTTG CATCCAGTAT TGTTCTGATC TTGTCACAAC GATCACTTTT CAAGTTTTAC 
ACGTACAGCT CAGCCTTTCT GTTAGCTGCA ACTTCAGTGT TGGTGAATTA TTATGCTTCT 
TTGCACATTG ACTTCTATGG TGCCTACAAC ACGTCAGCTT TTGGAATTGA GCTGCTTCCT 
CGAAAAGGTC CCTCGCTGTG GATGGCACTT ATCGTTCTAC AGCTAACATT TGGAATTGGA 
TACGTTACAC TACTCCAGAT TCATTCCATC TATTCACAAT TAATTATTTT GGATCTCTTG 
GTTCCTGTAA TAGGCTTAAT CACAGAGCTA CCATTACACA TCAGAGAGAC TTTACTGTTT 
ACTTCTTCCT TGATTCTCAC ATTAAATACA GTGTTTGTCC TGGCAGTGAA ACTGAAGTGG 
TTTTATTATT CCACACGATA TGTTTATCTT TTGGTGAGGC ACATGTATCG AATTTACGGA 
TTACAGTTAT TGATGGAGGA CACATGGAAG AGGATTCGTT TCCCAGACAT ACTACGAGTC 
TTTTGGCTAA CAAGAGTTAC AGCTCAGGCT ACAGTGTTAA TGTACATCTT AAGGATGGCA 
AATGAAACTG ATTCCTTCTT TATTTCTTGG GATGATTTTT GGGACCTCAT TTGCAATCTT 
ATAATTAGTG GGTGCGATTC TACACTAACT GTACTGGGCA TGAGTGCTGT AATTTCCTCA 
GTAGCCCATT ATTTGGGGCT TGGAATATTG GCCTTTATTG GATCAACTGA GGAAGATGAC 
AGGCGTCTTG GCTTTGTTGC ACCTGTTTTA TTTTTTATTT TGGCTCTTCA GACTGGGTTA 
AGTGGGCTAA GACCAGAAGA GAGACTTATT CGCTTAAGTA GAAACATGTG CCTTTTATTA 
ACTGCAGTCC TGCATTTTAT CCATGGAATG ACAGACCCTG TATTAATGTC TCTCAGTGCC 
TCTCATGTGT CATCTTTTCG TAGACATTTT CCTGTGCTGT TTGTCTCTGC TTGCCTGTTT 
ATTCTTCCTG TCTTACTCAG TTATGTTCTT TGGCATCACT ATGCACTAAA TACATGGTTG 
TTTGCAGTTA CAGCATTTTG TGTGGAACTG TGCTTAAAAG TAATTGTTTC TCTCACTGTT 
TATACGTTAT TCATGATTGA TGGCTACTAT AATGTCCTCT GGGAAAAGCT TGACGATTAT 
GTCTACTACG TTCGTTCAAC AGGCAGTATT ATTGAATTTA TATTTGGAGT TGTAATGTTT 
GGAAATGGGG CTTACACTAT GATGTTTGAG TCGGGAAGTA AAATTCGGGC TTTTATGATG 
TGCCTACATG CATATTTTAA CATCTACTTA CAAGCCAAAA ATGGCTGGAA GACATTTATG 
AATCGTAGGA CTGCTGTGAA GAAAATTAAT TCACTTCCTG AAATAAAAGG GAGCCGCTTA 
CAAGAAATAA ATGATGTATG TGCAATCTGC TATCATGAGT TTACAACATC TGCTCGTATT 
ACACCGTGTA ATCATTATTT CCATGCACTT TGCCTTCGGA AATGGCTGTA CATTCAAGAT 
ACTTGTCCAA TGTGCCATCA GAAAGTATAC ATCGAAGATG ATATCAAGGA TAATTCAAAT 
GTATCTAACA ACAATGGATT TATTCCACCC AATGAAACTC CAGAGGAAGC TGTAAGAGAA 
GCTGCTGCTG AATCTGACAG GGAATTGAAC GAAGATGACA GTACAGATTG TGATGATGAT 
GTTCAAAGAG AAAGAAATGG AGTGATTCAG CACACAGGCG CAGCAGCTGA AGAATTTAAT 
GATGATACTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF139 ( NM_007218.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_009149.2 Homo sapiens ring finger protein 139 (RNF139), mRNA.

Price & Availability↑

CloneID	OHu18432
Clone ID Related Accession (Same CDS sequence)	NM_007218.3 , NM_007218.4
Accession Version	NM_007218.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF139
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD171728.1, BC021571.1, BM804626.1, AA455970.1 and BM677460.1. On Nov 30, 2019 this sequence version replaced NM_007218.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064636.1, AF064801.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG TGGGGCCCCC GCAGCAGCAG GTGCGGATGG CCCATCAGCA GGTCTGGGCG 
GCGCTCGAAG TGGCGCTCCG GGTGCCCTGC CTTTACATCA TCGACGCCAT CTTCAACTCC 
TACCCGGATT CCAGCCAAAG CCGGTTCTGC ATCGTGCTCC AGATCTTCCT CCGGCTCTTT 
GGTGTATTTG CATCCAGTAT TGTTCTGATC TTGTCACAAC GATCACTTTT CAAGTTTTAC 
ACGTACAGCT CAGCCTTTCT GTTAGCTGCA ACTTCAGTGT TGGTGAATTA TTATGCTTCT 
TTGCACATTG ACTTCTATGG TGCCTACAAC ACGTCAGCTT TTGGAATTGA GCTGCTTCCT 
CGAAAAGGTC CCTCGCTGTG GATGGCACTT ATCGTTCTAC AGCTAACATT TGGAATTGGA 
TACGTTACAC TACTCCAGAT TCATTCCATC TATTCACAAT TAATTATTTT GGATCTCTTG 
GTTCCTGTAA TAGGCTTAAT CACAGAGCTA CCATTACACA TCAGAGAGAC TTTACTGTTT 
ACTTCTTCCT TGATTCTCAC ATTAAATACA GTGTTTGTCC TGGCAGTGAA ACTGAAGTGG 
TTTTATTATT CCACACGATA TGTTTATCTT TTGGTGAGGC ACATGTATCG AATTTACGGA 
TTACAGTTAT TGATGGAGGA CACATGGAAG AGGATTCGTT TCCCAGACAT ACTACGAGTC 
TTTTGGCTAA CAAGAGTTAC AGCTCAGGCT ACAGTGTTAA TGTACATCTT AAGGATGGCA 
AATGAAACTG ATTCCTTCTT TATTTCTTGG GATGATTTTT GGGACCTCAT TTGCAATCTT 
ATAATTAGTG GGTGCGATTC TACACTAACT GTACTGGGCA TGAGTGCTGT AATTTCCTCA 
GTAGCCCATT ATTTGGGGCT TGGAATATTG GCCTTTATTG GATCAACTGA GGAAGATGAC 
AGGCGTCTTG GCTTTGTTGC ACCTGTTTTA TTTTTTATTT TGGCTCTTCA GACTGGGTTA 
AGTGGGCTAA GACCAGAAGA GAGACTTATT CGCTTAAGTA GAAACATGTG CCTTTTATTA 
ACTGCAGTCC TGCATTTTAT CCATGGAATG ACAGACCCTG TATTAATGTC TCTCAGTGCC 
TCTCATGTGT CATCTTTTCG TAGACATTTT CCTGTGCTGT TTGTCTCTGC TTGCCTGTTT 
ATTCTTCCTG TCTTACTCAG TTATGTTCTT TGGCATCACT ATGCACTAAA TACATGGTTG 
TTTGCAGTTA CAGCATTTTG TGTGGAACTG TGCTTAAAAG TAATTGTTTC TCTCACTGTT 
TATACGTTAT TCATGATTGA TGGCTACTAT AATGTCCTCT GGGAAAAGCT TGACGATTAT 
GTCTACTACG TTCGTTCAAC AGGCAGTATT ATTGAATTTA TATTTGGAGT TGTAATGTTT 
GGAAATGGGG CTTACACTAT GATGTTTGAG TCGGGAAGTA AAATTCGGGC TTTTATGATG 
TGCCTACATG CATATTTTAA CATCTACTTA CAAGCCAAAA ATGGCTGGAA GACATTTATG 
AATCGTAGGA CTGCTGTGAA GAAAATTAAT TCACTTCCTG AAATAAAAGG GAGCCGCTTA 
CAAGAAATAA ATGATGTATG TGCAATCTGC TATCATGAGT TTACAACATC TGCTCGTATT 
ACACCGTGTA ATCATTATTT CCATGCACTT TGCCTTCGGA AATGGCTGTA CATTCAAGAT 
ACTTGTCCAA TGTGCCATCA GAAAGTATAC ATCGAAGATG ATATCAAGGA TAATTCAAAT 
GTATCTAACA ACAATGGATT TATTCCACCC AATGAAACTC CAGAGGAAGC TGTAAGAGAA 
GCTGCTGCTG AATCTGACAG GGAATTGAAC GAAGATGACA GTACAGATTG TGATGATGAT 
GTTCAAAGAG AAAGAAATGG AGTGATTCAG CACACAGGCG CAGCAGCTGA AGAATTTAAT 
GATGATACTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009149.2
CDS	231..2225
Translation	MAAVGPPQQQVRMAHQQVWAALEVALRVPCLYIIDAIFNSYPDSSQSRFCIVLQIFLRLFGVFASSIVLILSQRSLFKFYTYSSAFLLAATSVLVNYYASLHIDFYGAYNTSAFGIELLPRKGPSLWMALIVLQLTFGIGYVTLLQIHSIYSQLIILDLLVPVIGLITELPLHIRETLLFTSSLILTLNTVFVLAVKLKWFYYSTRYVYLLVRHMYRIYGLQLLMEDTWKRIRFPDILRVFWLTRVTAQATVLMYILRMANETDSFFISWDDFWDLICNLIISGCDSTLTVLGMSAVISSVAHYLGLGILAFIGSTEEDDRRLGFVAPVLFFILALQTGLSGLRPEERLIRLSRNMCLLLTAVLHFIHGMTDPVLMSLSASHVSSFRRHFPVLFVSACLFILPVLLSYVLWHHYALNTWLFAVTAFCVELCLKVIVSLTVYTLFMIDGYYNVLWEKLDDYVYYVRSTGSIIEFIFGVVMFGNGAYTMMFESGSKIRAFMMCLHAYFNIYLQAKNGWKTFMNRRTAVKKINSLPEIKGSRLQEINDVCAICYHEFTTSARITPCNHYFHALCLRKWLYIQDTCPMCHQKVYIEDDIKDNSNVSNNNGFIPPNETPEEAVREAAAESDRELNEDDSTDCDDDVQRERNGVIQHTGAAAEEFNDDTD

Target ORF information:

RefSeq Version	NM_007218.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 139 (RNF139), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007218.4

ORF Insert Sequence:

ATGGCGGCCG TGGGGCCCCC GCAGCAGCAG GTGCGGATGG CCCATCAGCA GGTCTGGGCG 
GCGCTCGAAG TGGCGCTCCG GGTGCCCTGC CTTTACATCA TCGACGCCAT CTTCAACTCC 
TACCCGGATT CCAGCCAAAG CCGGTTCTGC ATCGTGCTCC AGATCTTCCT CCGGCTCTTT 
GGTGTATTTG CATCCAGTAT TGTTCTGATC TTGTCACAAC GATCACTTTT CAAGTTTTAC 
ACGTACAGCT CAGCCTTTCT GTTAGCTGCA ACTTCAGTGT TGGTGAATTA TTATGCTTCT 
TTGCACATTG ACTTCTATGG TGCCTACAAC ACGTCAGCTT TTGGAATTGA GCTGCTTCCT 
CGAAAAGGTC CCTCGCTGTG GATGGCACTT ATCGTTCTAC AGCTAACATT TGGAATTGGA 
TACGTTACAC TACTCCAGAT TCATTCCATC TATTCACAAT TAATTATTTT GGATCTCTTG 
GTTCCTGTAA TAGGCTTAAT CACAGAGCTA CCATTACACA TCAGAGAGAC TTTACTGTTT 
ACTTCTTCCT TGATTCTCAC ATTAAATACA GTGTTTGTCC TGGCAGTGAA ACTGAAGTGG 
TTTTATTATT CCACACGATA TGTTTATCTT TTGGTGAGGC ACATGTATCG AATTTACGGA 
TTACAGTTAT TGATGGAGGA CACATGGAAG AGGATTCGTT TCCCAGACAT ACTACGAGTC 
TTTTGGCTAA CAAGAGTTAC AGCTCAGGCT ACAGTGTTAA TGTACATCTT AAGGATGGCA 
AATGAAACTG ATTCCTTCTT TATTTCTTGG GATGATTTTT GGGACCTCAT TTGCAATCTT 
ATAATTAGTG GGTGCGATTC TACACTAACT GTACTGGGCA TGAGTGCTGT AATTTCCTCA 
GTAGCCCATT ATTTGGGGCT TGGAATATTG GCCTTTATTG GATCAACTGA GGAAGATGAC 
AGGCGTCTTG GCTTTGTTGC ACCTGTTTTA TTTTTTATTT TGGCTCTTCA GACTGGGTTA 
AGTGGGCTAA GACCAGAAGA GAGACTTATT CGCTTAAGTA GAAACATGTG CCTTTTATTA 
ACTGCAGTCC TGCATTTTAT CCATGGAATG ACAGACCCTG TATTAATGTC TCTCAGTGCC 
TCTCATGTGT CATCTTTTCG TAGACATTTT CCTGTGCTGT TTGTCTCTGC TTGCCTGTTT 
ATTCTTCCTG TCTTACTCAG TTATGTTCTT TGGCATCACT ATGCACTAAA TACATGGTTG 
TTTGCAGTTA CAGCATTTTG TGTGGAACTG TGCTTAAAAG TAATTGTTTC TCTCACTGTT 
TATACGTTAT TCATGATTGA TGGCTACTAT AATGTCCTCT GGGAAAAGCT TGACGATTAT 
GTCTACTACG TTCGTTCAAC AGGCAGTATT ATTGAATTTA TATTTGGAGT TGTAATGTTT 
GGAAATGGGG CTTACACTAT GATGTTTGAG TCGGGAAGTA AAATTCGGGC TTTTATGATG 
TGCCTACATG CATATTTTAA CATCTACTTA CAAGCCAAAA ATGGCTGGAA GACATTTATG 
AATCGTAGGA CTGCTGTGAA GAAAATTAAT TCACTTCCTG AAATAAAAGG GAGCCGCTTA 
CAAGAAATAA ATGATGTATG TGCAATCTGC TATCATGAGT TTACAACATC TGCTCGTATT 
ACACCGTGTA ATCATTATTT CCATGCACTT TGCCTTCGGA AATGGCTGTA CATTCAAGAT 
ACTTGTCCAA TGTGCCATCA GAAAGTATAC ATCGAAGATG ATATCAAGGA TAATTCAAAT 
GTATCTAACA ACAATGGATT TATTCCACCC AATGAAACTC CAGAGGAAGC TGTAAGAGAA 
GCTGCTGCTG AATCTGACAG GGAATTGAAC GAAGATGACA GTACAGATTG TGATGATGAT 
GTTCAAAGAG AAAGAAATGG AGTGATTCAG CACACAGGCG CAGCAGCTGA AGAATTTAAT 
GATGATACTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF139 ( XM_017013002.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868491.1 Homo sapiens ring finger protein 139 (RNF139), transcript variant X1, mRNA.

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CloneID	OHu97718
Clone ID Related Accession (Same CDS sequence)	XM_017013002.1
Accession Version	XM_017013002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF139 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTTA TCGTTCTACA GCTAACATTT GGAATTGGAT ACGTTACACT ACTCCAGATT 
CATTCCATCT ATTCACAATT AATTATTTTG GATCTCTTGG TTCCTGTAAT AGGCTTAATC 
ACAGAGCTAC CATTACACAT CAGAGAGACT TTACTGTTTA CTTCTTCCTT GATTCTCACA 
TTAAATACAG TGTTTGTCCT GGCAGTGAAA CTGAAGTGGT TTTATTATTC CACACGATAT 
GTTTATCTTT TGGTGAGGCA CATGTATCGA ATTTACGGAT TACAGTTATT GATGGAGGAC 
ACATGGAAGA GGATTCGTTT CCCAGACATA CTACGAGTCT TTTGGCTAAC AAGAGTTACA 
GCTCAGGCTA CAGTGTTAAT GTACATCTTA AGGATGGCAA ATGAAACTGA TTCCTTCTTT 
ATTTCTTGGG ATGATTTTTG GGACCTCATT TGCAATCTTA TAATTAGTGG GTGCGATTCT 
ACACTAACTG TACTGGGCAT GAGTGCTGTA ATTTCCTCAG TAGCCCATTA TTTGGGGCTT 
GGAATATTGG CCTTTATTGG ATCAACTGAG GAAGATGACA GGCGTCTTGG CTTTGTTGCA 
CCTGTTTTAT TTTTTATTTT GGCTCTTCAG ACTGGGTTAA GTGGGCTAAG ACCAGAAGAG 
AGACTTATTC GCTTAAGTAG AAACATGTGC CTTTTATTAA CTGCAGTCCT GCATTTTATC 
CATGGAATGA CAGACCCTGT ATTAATGTCT CTCAGTGCCT CTCATGTGTC ATCTTTTCGT 
AGACATTTTC CTGTGCTGTT TGTCTCTGCT TGCCTGTTTA TTCTTCCTGT CTTACTCAGT 
TATGTTCTTT GGCATCACTA TGCACTAAAT ACATGGTTGT TTGCAGTTAC AGCATTTTGT 
GTGGAACTGT GCTTAAAAGT AATTGTTTCT CTCACTGTTT ATACGTTATT CATGATTGAT 
GGCTACTATA ATGTCCTCTG GGAAAAGCTT GACGATTATG TCTACTACGT TCGTTCAACA 
GGCAGTATTA TTGAATTTAT ATTTGGAGTT GTAATGTTTG GAAATGGGGC TTACACTATG 
ATGTTTGAGT CGGGAAGTAA AATTCGGGCT TTTATGATGT GCCTACATGC ATATTTTAAC 
ATCTACTTAC AAGCCAAAAA TGGCTGGAAG ACATTTATGA ATCGTAGGAC TGCTGTGAAG 
AAAATTAATT CACTTCCTGA AATAAAAGGG AGCCGCTTAC AAGAAATAAA TGATGTATGT 
GCAATCTGCT ATCATGAGTT TACAACATCT GCTCGTATTA CACCGTGTAA TCATTATTTC 
CATGCACTTT GCCTTCGGAA ATGGCTGTAC ATTCAAGATA CTTGTCCAAT GTGCCATCAG 
AAAGTATACA TCGAAGATGA TATCAAGGAT AATTCAAATG TATCTAACAA CAATGGATTT 
ATTCCACCCA ATGAAACTCC AGAGGAAGCT GTAAGAGAAG CTGCTGCTGA ATCTGACAGG 
GAATTGAACG AAGATGACAG TACAGATTGT GATGATGATG TTCAAAGAGA AAGAAATGGA 
GTGATTCAGC ACACAGGCGC AGCAGCTGAA GAATTTAATG ATGATACTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868491.1
CDS	415..2028
Translation	MALIVLQLTFGIGYVTLLQIHSIYSQLIILDLLVPVIGLITELPLHIRETLLFTSSLILTLNTVFVLAVKLKWFYYSTRYVYLLVRHMYRIYGLQLLMEDTWKRIRFPDILRVFWLTRVTAQATVLMYILRMANETDSFFISWDDFWDLICNLIISGCDSTLTVLGMSAVISSVAHYLGLGILAFIGSTEEDDRRLGFVAPVLFFILALQTGLSGLRPEERLIRLSRNMCLLLTAVLHFIHGMTDPVLMSLSASHVSSFRRHFPVLFVSACLFILPVLLSYVLWHHYALNTWLFAVTAFCVELCLKVIVSLTVYTLFMIDGYYNVLWEKLDDYVYYVRSTGSIIEFIFGVVMFGNGAYTMMFESGSKIRAFMMCLHAYFNIYLQAKNGWKTFMNRRTAVKKINSLPEIKGSRLQEINDVCAICYHEFTTSARITPCNHYFHALCLRKWLYIQDTCPMCHQKVYIEDDIKDNSNVSNNNGFIPPNETPEEAVREAAAESDRELNEDDSTDCDDDVQRERNGVIQHTGAAAEEFNDDTD

Target ORF information:

RefSeq Version	XM_017013002.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 139 (RNF139), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013002.1

ORF Insert Sequence:

ATGGCACTTA TCGTTCTACA GCTAACATTT GGAATTGGAT ACGTTACACT ACTCCAGATT 
CATTCCATCT ATTCACAATT AATTATTTTG GATCTCTTGG TTCCTGTAAT AGGCTTAATC 
ACAGAGCTAC CATTACACAT CAGAGAGACT TTACTGTTTA CTTCTTCCTT GATTCTCACA 
TTAAATACAG TGTTTGTCCT GGCAGTGAAA CTGAAGTGGT TTTATTATTC CACACGATAT 
GTTTATCTTT TGGTGAGGCA CATGTATCGA ATTTACGGAT TACAGTTATT GATGGAGGAC 
ACATGGAAGA GGATTCGTTT CCCAGACATA CTACGAGTCT TTTGGCTAAC AAGAGTTACA 
GCTCAGGCTA CAGTGTTAAT GTACATCTTA AGGATGGCAA ATGAAACTGA TTCCTTCTTT 
ATTTCTTGGG ATGATTTTTG GGACCTCATT TGCAATCTTA TAATTAGTGG GTGCGATTCT 
ACACTAACTG TACTGGGCAT GAGTGCTGTA ATTTCCTCAG TAGCCCATTA TTTGGGGCTT 
GGAATATTGG CCTTTATTGG ATCAACTGAG GAAGATGACA GGCGTCTTGG CTTTGTTGCA 
CCTGTTTTAT TTTTTATTTT GGCTCTTCAG ACTGGGTTAA GTGGGCTAAG ACCAGAAGAG 
AGACTTATTC GCTTAAGTAG AAACATGTGC CTTTTATTAA CTGCAGTCCT GCATTTTATC 
CATGGAATGA CAGACCCTGT ATTAATGTCT CTCAGTGCCT CTCATGTGTC ATCTTTTCGT 
AGACATTTTC CTGTGCTGTT TGTCTCTGCT TGCCTGTTTA TTCTTCCTGT CTTACTCAGT 
TATGTTCTTT GGCATCACTA TGCACTAAAT ACATGGTTGT TTGCAGTTAC AGCATTTTGT 
GTGGAACTGT GCTTAAAAGT AATTGTTTCT CTCACTGTTT ATACGTTATT CATGATTGAT 
GGCTACTATA ATGTCCTCTG GGAAAAGCTT GACGATTATG TCTACTACGT TCGTTCAACA 
GGCAGTATTA TTGAATTTAT ATTTGGAGTT GTAATGTTTG GAAATGGGGC TTACACTATG 
ATGTTTGAGT CGGGAAGTAA AATTCGGGCT TTTATGATGT GCCTACATGC ATATTTTAAC 
ATCTACTTAC AAGCCAAAAA TGGCTGGAAG ACATTTATGA ATCGTAGGAC TGCTGTGAAG 
AAAATTAATT CACTTCCTGA AATAAAAGGG AGCCGCTTAC AAGAAATAAA TGATGTATGT 
GCAATCTGCT ATCATGAGTT TACAACATCT GCTCGTATTA CACCGTGTAA TCATTATTTC 
CATGCACTTT GCCTTCGGAA ATGGCTGTAC ATTCAAGATA CTTGTCCAAT GTGCCATCAG 
AAAGTATACA TCGAAGATGA TATCAAGGAT AATTCAAATG TATCTAACAA CAATGGATTT 
ATTCCACCCA ATGAAACTCC AGAGGAAGCT GTAAGAGAAG CTGCTGCTGA ATCTGACAGG 
GAATTGAACG AAGATGACAG TACAGATTGT GATGATGATG TTCAAAGAGA AAGAAATGGA 
GTGATTCAGC ACACAGGCGC AGCAGCTGAA GAATTTAATG ATGATACTGA CTGA