EEF1AKMT4-ECE2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEF1AKMT4-ECE2
Entrez Gene ID	110599583
Full Name	EEF1AKMT4-ECE2 readthrough
General protein information	Preferred Names EEF1AKMT4-ECE2 readthrough Names EEF1AKMT4-ECE2 readthrough
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q27.1
Summary	This gene represents naturally occurring readthrough transcription between adjacent genes eukaryotic translation elongation factor 1 alpha lysine methyltransferase 4 (GeneID: 110599564) and endothelin converting enzyme 2 (GeneID:9718). The readthrough transcript representing this gene encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Jul 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_014693.4	NP_055508.3	EEF1AKMT4-ECE2 readthrough transcript protein
NM_014693.3	NP_055508.3	EEF1AKMT4-ECE2 readthrough transcript protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Methylation of human eukaryotic elongation factor alpha (eEF1A) by a member of a novel protein lysine methyltransferase family modulates mRNA translation.
Jakobsson ME, Malecki J, Nilges BS, Moen A, Leidel SA, Falnes P?
Nucleic acids research45(14)8239-8254(2017 Aug)

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A
Genome research13(10)2265-70(2003 Oct)

Human endothelin converting enzyme-2 (ECE2): characterization of mRNA species and chromosomal localization.
Lorenzo MN, Khan RY, Wang Y, Tai SC, Chan GC, Cheung AH, Marsden PA
Biochimica et biophysica acta1522(1)46-52(2001 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EEF1AKMT4-ECE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF1AKMT4-ECE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18245	NM_014693.4 Latest version!	Homo sapiens EEF1AKMT4-ECE2 readthrough (EEF1AKMT4-ECE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$444.50-$622.30 $889.00
View Old Accession Versions >>
OHu18245	NM_014693.3	Homo sapiens EEF1AKMT4-ECE2 readthrough (EEF1AKMT4-ECE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$444.50-$622.30 $889.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEF1AKMT4-ECE2 ( NM_014693.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055508.3 Homo sapiens EEF1AKMT4-ECE2 readthrough (EEF1AKMT4-ECE2), mRNA.

Price & Availability↑

CloneID	OHu18245
Clone ID Related Accession (Same CDS sequence)	NM_014693.4 , NM_014693.3
Accession Version	NM_014693.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	EEF1AKMT4-ECE2 readthrough transcript protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU568980.1, AF428263.1, AY359003.1 and BC142950.1. On Jun 2, 2019 this sequence version replaced NM_014693.3. ##Evidence-Data-START## Transcript exon combination :: AF428263.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 9718, 110599564 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCTC CAGGGGCAGG TAGGGCGCCT CCGGAGTTAC CGGAGCGGAA CTGCGGGTAC 
CGCGAAGTCG AGTACTGGGA TCAGCGCTAC CAAGGCGCAG CCGATTCTGC CCCCTACGAT 
TGGTTCGGGG ACTTCTCCTC CTTCCGTGCC CTCCTAGAGC CGGAGCTGCG GCCCGAGGAC 
CGTATCCTTG TGCTAGGTTG CGGGAACAGT GCCCTGAGCT ACGAGCTGTT CCTCGGAGGC 
TTCCCTAATG TGACCAGTGT GGACTACTCA TCAGTCGTGG TGGCTGCCAT GCAGGCTCGC 
CATGCCCATG TGCCGCAGCT GCGCTGGGAG ACCATGGATG TGCGGAAGCT GGACTTCCCC 
AGTGCTTCTT TTGATGTGGT GCTCGAGAAG GGCACGCTGG ATGCCCTGCT GGCTGGGGAA 
CGAGATCCCT GGACCGTGTC CTCTGAAGGT GTCCACACTG TGGACCAGGT GTTGAGTGAG 
GTGGGATTCC AGAAGGGGAC AAGACAGCTG TTAGGCTCAC GCACGCAGCT GGAGCTGGTC 
TTAGCAGGTG CCTCTCTACT GCTGGCTGCA CTGCTTCTGG GCTGCCTTGT GGCCCTAGGG 
GTCCAGTACC ACAGAGACCC ATCCCACAGC ACCTGCCTTA CAGAGGCCTG CATTCGAGTG 
GCTGGAAAAA TCCTGGAGTC CCTGGACCGA GGGGTGAGCC CCTGTGAGGA CTTTTACCAG 
TTCTCCTGTG GGGGCTGGAT TCGGAGGAAC CCCCTGCCCG ATGGGCGTTC TCGCTGGAAC 
ACCTTCAACA GCCTCTGGGA CCAAAACCAG GCCATACTGA AGCACCTGCT TGAAAACACC 
ACCTTCAACT CCAGCAGTGA AGCTGAGCAG AAGACACAGC GCTTCTACCT ATCTTGCCTA 
CAGGTGGAGC GCATTGAGGA GCTGGGAGCC CAGCCACTGA GAGACCTCAT TGAGAAGATT 
GGTGGTTGGA ACATTACGGG GCCCTGGGAC CAGGACAACT TTATGGAGGT GTTGAAGGCA 
GTAGCAGGGA CCTACAGGGC CACCCCATTC TTCACCGTCT ACATCAGTGC CGACTCTAAG 
AGTTCCAACA GCAATGTTAT CCAGGTGGAC CAGTCTGGGC TCTTTCTGCC CTCTCGGGAT 
TACTACTTAA ACAGAACTGC CAATGAGAAA GTGCTCACTG CCTATCTGGA TTACATGGAG 
GAACTGGGGA TGCTGCTGGG TGGGCGGCCC ACCTCCACGA GGGAGCAGAT GCAGCAGGTG 
CTGGAGTTGG AGATACAGCT GGCCAACATC ACAGTGCCCC AGGACCAGCG GCGCGACGAG 
GAGAAGATCT ACCACAAGAT GAGCATTTCG GAGCTGCAGG CTCTGGCGCC CTCCATGGAC 
TGGCTTGAGT TCCTGTCTTT CTTGCTGTCA CCATTGGAGT TGAGTGACTC TGAGCCTGTG 
GTGGTGTATG GGATGGATTA TTTGCAGCAG GTGTCAGAGC TCATCAACCG CACGGAACCA 
AGCATCCTGA ACAATTACCT GATCTGGAAC CTGGTGCAAA AGACAACCTC AAGCCTGGAC 
CGACGCTTTG AGTCTGCACA AGAGAAGCTG CTGGAGACCC TCTATGGCAC TAAGAAGTCC 
TGTGTGCCGA GGTGGCAGAC CTGCATCTCC AACACGGATG ACGCCCTTGG CTTTGCTTTG 
GGGTCCCTCT TCGTGAAGGC CACGTTTGAC CGGCAAAGCA AAGAAATTGC AGAGGGGATG 
ATCAGCGAAA TCCGGACCGC ATTTGAGGAG GCCCTGGGAC AGCTGGTTTG GATGGATGAG 
AAGACCCGCC AGGCAGCCAA GGAGAAAGCA GATGCCATCT ATGATATGAT TGGTTTCCCA 
GACTTTATCC TGGAGCCCAA AGAGCTGGAT GATGTTTATG ACGGGTACGA AATTTCTGAA 
GATTCTTTCT TCCAAAACAT GTTGAATTTG TACAACTTCT CTGCCAAGGT TATGGCTGAC 
CAGCTCCGCA AGCCTCCCAG CCGAGACCAG TGGAGCATGA CCCCCCAGAC AGTGAATGCC 
TACTACCTTC CAACTAAGAA TGAGATCGTC TTCCCCGCTG GCATCCTGCA GGCCCCCTTC 
TATGCCCGCA ACCACCCCAA GGCCCTGAAC TTCGGTGGCA TCGGTGTGGT CATGGGCCAT 
GAGTTGACGC ATGCCTTTGA TGACCAAGGG CGCGAGTATG ACAAAGAAGG GAACCTGCGG 
CCCTGGTGGC AGAATGAGTC CCTGGCAGCC TTCCGGAACC ACACGGCCTG CATGGAGGAA 
CAGTACAATC AATACCAGGT CAATGGGGAG AGGCTCAACG GCCGCCAGAC GCTGGGGGAG 
AACATTGCTG ACAACGGGGG GCTGAAGGCT GCCTACAATG CTTACAAAGC ATGGCTGAGA 
AAGCATGGGG AGGAGCAGCA ACTGCCAGCC GTGGGGCTCA CCAACCACCA GCTCTTCTTC 
GTGGGATTTG CCCAGGTGTG GTGCTCGGTC CGCACACCAG AGAGCTCTCA CGAGGGGCTG 
GTGACCGACC CCCACAGCCC TGCCCGCTTC CGCGTGCTGG GCACTCTCTC CAACTCCCGT 
GACTTCCTGC GGCACTTCGG CTGCCCTGTC GGCTCCCCCA TGAACCCAGG GCAGCTGTGT 
GAGGTGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055508.3
CDS	24..2675
Translation	MASPGAGRAPPELPERNCGYREVEYWDQRYQGAADSAPYDWFGDFSSFRALLEPELRPEDRILVLGCGNSALSYELFLGGFPNVTSVDYSSVVVAAMQARHAHVPQLRWETMDVRKLDFPSASFDVVLEKGTLDALLAGERDPWTVSSEGVHTVDQVLSEVGFQKGTRQLLGSRTQLELVLAGASLLLAALLLGCLVALGVQYHRDPSHSTCLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAEQKTQRFYLSCLQVERIEELGAQPLRDLIEKIGGWNITGPWDQDNFMEVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGERLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW

Target ORF information:

RefSeq Version	NM_014693.4
Organism	Homo sapiens(human)
Definition	Homo sapiens EEF1AKMT4-ECE2 readthrough (EEF1AKMT4-ECE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014693.4

ORF Insert Sequence:

ATGGCCTCTC CAGGGGCAGG TAGGGCGCCT CCGGAGTTAC CGGAGCGGAA CTGCGGGTAC 
CGCGAAGTCG AGTACTGGGA TCAGCGCTAC CAAGGCGCAG CCGATTCTGC CCCCTACGAT 
TGGTTCGGGG ACTTCTCCTC CTTCCGTGCC CTCCTAGAGC CGGAGCTGCG GCCCGAGGAC 
CGTATCCTTG TGCTAGGTTG CGGGAACAGT GCCCTGAGCT ACGAGCTGTT CCTCGGAGGC 
TTCCCTAATG TGACCAGTGT GGACTACTCA TCAGTCGTGG TGGCTGCCAT GCAGGCTCGC 
CATGCCCATG TGCCGCAGCT GCGCTGGGAG ACCATGGATG TGCGGAAGCT GGACTTCCCC 
AGTGCTTCTT TTGATGTGGT GCTCGAGAAG GGCACGCTGG ATGCCCTGCT GGCTGGGGAA 
CGAGATCCCT GGACCGTGTC CTCTGAAGGT GTCCACACTG TGGACCAGGT GTTGAGTGAG 
GTGGGATTCC AGAAGGGGAC AAGACAGCTG TTAGGCTCAC GCACGCAGCT GGAGCTGGTC 
TTAGCAGGTG CCTCTCTACT GCTGGCTGCA CTGCTTCTGG GCTGCCTTGT GGCCCTAGGG 
GTCCAGTACC ACAGAGACCC ATCCCACAGC ACCTGCCTTA CAGAGGCCTG CATTCGAGTG 
GCTGGAAAAA TCCTGGAGTC CCTGGACCGA GGGGTGAGCC CCTGTGAGGA CTTTTACCAG 
TTCTCCTGTG GGGGCTGGAT TCGGAGGAAC CCCCTGCCCG ATGGGCGTTC TCGCTGGAAC 
ACCTTCAACA GCCTCTGGGA CCAAAACCAG GCCATACTGA AGCACCTGCT TGAAAACACC 
ACCTTCAACT CCAGCAGTGA AGCTGAGCAG AAGACACAGC GCTTCTACCT ATCTTGCCTA 
CAGGTGGAGC GCATTGAGGA GCTGGGAGCC CAGCCACTGA GAGACCTCAT TGAGAAGATT 
GGTGGTTGGA ACATTACGGG GCCCTGGGAC CAGGACAACT TTATGGAGGT GTTGAAGGCA 
GTAGCAGGGA CCTACAGGGC CACCCCATTC TTCACCGTCT ACATCAGTGC CGACTCTAAG 
AGTTCCAACA GCAATGTTAT CCAGGTGGAC CAGTCTGGGC TCTTTCTGCC CTCTCGGGAT 
TACTACTTAA ACAGAACTGC CAATGAGAAA GTGCTCACTG CCTATCTGGA TTACATGGAG 
GAACTGGGGA TGCTGCTGGG TGGGCGGCCC ACCTCCACGA GGGAGCAGAT GCAGCAGGTG 
CTGGAGTTGG AGATACAGCT GGCCAACATC ACAGTGCCCC AGGACCAGCG GCGCGACGAG 
GAGAAGATCT ACCACAAGAT GAGCATTTCG GAGCTGCAGG CTCTGGCGCC CTCCATGGAC 
TGGCTTGAGT TCCTGTCTTT CTTGCTGTCA CCATTGGAGT TGAGTGACTC TGAGCCTGTG 
GTGGTGTATG GGATGGATTA TTTGCAGCAG GTGTCAGAGC TCATCAACCG CACGGAACCA 
AGCATCCTGA ACAATTACCT GATCTGGAAC CTGGTGCAAA AGACAACCTC AAGCCTGGAC 
CGACGCTTTG AGTCTGCACA AGAGAAGCTG CTGGAGACCC TCTATGGCAC TAAGAAGTCC 
TGTGTGCCGA GGTGGCAGAC CTGCATCTCC AACACGGATG ACGCCCTTGG CTTTGCTTTG 
GGGTCCCTCT TCGTGAAGGC CACGTTTGAC CGGCAAAGCA AAGAAATTGC AGAGGGGATG 
ATCAGCGAAA TCCGGACCGC ATTTGAGGAG GCCCTGGGAC AGCTGGTTTG GATGGATGAG 
AAGACCCGCC AGGCAGCCAA GGAGAAAGCA GATGCCATCT ATGATATGAT TGGTTTCCCA 
GACTTTATCC TGGAGCCCAA AGAGCTGGAT GATGTTTATG ACGGGTACGA AATTTCTGAA 
GATTCTTTCT TCCAAAACAT GTTGAATTTG TACAACTTCT CTGCCAAGGT TATGGCTGAC 
CAGCTCCGCA AGCCTCCCAG CCGAGACCAG TGGAGCATGA CCCCCCAGAC AGTGAATGCC 
TACTACCTTC CAACTAAGAA TGAGATCGTC TTCCCCGCTG GCATCCTGCA GGCCCCCTTC 
TATGCCCGCA ACCACCCCAA GGCCCTGAAC TTCGGTGGCA TCGGTGTGGT CATGGGCCAT 
GAGTTGACGC ATGCCTTTGA TGACCAAGGG CGCGAGTATG ACAAAGAAGG GAACCTGCGG 
CCCTGGTGGC AGAATGAGTC CCTGGCAGCC TTCCGGAACC ACACGGCCTG CATGGAGGAA 
CAGTACAATC AATACCAGGT CAATGGGGAG AGGCTCAACG GCCGCCAGAC GCTGGGGGAG 
AACATTGCTG ACAACGGGGG GCTGAAGGCT GCCTACAATG CTTACAAAGC ATGGCTGAGA 
AAGCATGGGG AGGAGCAGCA ACTGCCAGCC GTGGGGCTCA CCAACCACCA GCTCTTCTTC 
GTGGGATTTG CCCAGGTGTG GTGCTCGGTC CGCACACCAG AGAGCTCTCA CGAGGGGCTG 
GTGACCGACC CCCACAGCCC TGCCCGCTTC CGCGTGCTGG GCACTCTCTC CAACTCCCGT 
GACTTCCTGC GGCACTTCGG CTGCCCTGTC GGCTCCCCCA TGAACCCAGG GCAGCTGTGT 
GAGGTGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF1AKMT4-ECE2 ( NM_014693.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055508.3 Homo sapiens EEF1AKMT4-ECE2 readthrough (EEF1AKMT4-ECE2), mRNA.

Price & Availability↑

CloneID	OHu18245
Clone ID Related Accession (Same CDS sequence)	NM_014693.4 , NM_014693.3
Accession Version	NM_014693.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	EEF1AKMT4-ECE2 readthrough transcript protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU568980.1, AF428263.1, AY359003.1 and BC142950.1. On Jul 26, 2007 this sequence version replaced NM_014693.2. ##Evidence-Data-START## Transcript exon combination :: AF428263.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 9718, 110599564 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCTC CAGGGGCAGG TAGGGCGCCT CCGGAGTTAC CGGAGCGGAA CTGCGGGTAC 
CGCGAAGTCG AGTACTGGGA TCAGCGCTAC CAAGGCGCAG CCGATTCTGC CCCCTACGAT 
TGGTTCGGGG ACTTCTCCTC CTTCCGTGCC CTCCTAGAGC CGGAGCTGCG GCCCGAGGAC 
CGTATCCTTG TGCTAGGTTG CGGGAACAGT GCCCTGAGCT ACGAGCTGTT CCTCGGAGGC 
TTCCCTAATG TGACCAGTGT GGACTACTCA TCAGTCGTGG TGGCTGCCAT GCAGGCTCGC 
CATGCCCATG TGCCGCAGCT GCGCTGGGAG ACCATGGATG TGCGGAAGCT GGACTTCCCC 
AGTGCTTCTT TTGATGTGGT GCTCGAGAAG GGCACGCTGG ATGCCCTGCT GGCTGGGGAA 
CGAGATCCCT GGACCGTGTC CTCTGAAGGT GTCCACACTG TGGACCAGGT GTTGAGTGAG 
GTGGGATTCC AGAAGGGGAC AAGACAGCTG TTAGGCTCAC GCACGCAGCT GGAGCTGGTC 
TTAGCAGGTG CCTCTCTACT GCTGGCTGCA CTGCTTCTGG GCTGCCTTGT GGCCCTAGGG 
GTCCAGTACC ACAGAGACCC ATCCCACAGC ACCTGCCTTA CAGAGGCCTG CATTCGAGTG 
GCTGGAAAAA TCCTGGAGTC CCTGGACCGA GGGGTGAGCC CCTGTGAGGA CTTTTACCAG 
TTCTCCTGTG GGGGCTGGAT TCGGAGGAAC CCCCTGCCCG ATGGGCGTTC TCGCTGGAAC 
ACCTTCAACA GCCTCTGGGA CCAAAACCAG GCCATACTGA AGCACCTGCT TGAAAACACC 
ACCTTCAACT CCAGCAGTGA AGCTGAGCAG AAGACACAGC GCTTCTACCT ATCTTGCCTA 
CAGGTGGAGC GCATTGAGGA GCTGGGAGCC CAGCCACTGA GAGACCTCAT TGAGAAGATT 
GGTGGTTGGA ACATTACGGG GCCCTGGGAC CAGGACAACT TTATGGAGGT GTTGAAGGCA 
GTAGCAGGGA CCTACAGGGC CACCCCATTC TTCACCGTCT ACATCAGTGC CGACTCTAAG 
AGTTCCAACA GCAATGTTAT CCAGGTGGAC CAGTCTGGGC TCTTTCTGCC CTCTCGGGAT 
TACTACTTAA ACAGAACTGC CAATGAGAAA GTGCTCACTG CCTATCTGGA TTACATGGAG 
GAACTGGGGA TGCTGCTGGG TGGGCGGCCC ACCTCCACGA GGGAGCAGAT GCAGCAGGTG 
CTGGAGTTGG AGATACAGCT GGCCAACATC ACAGTGCCCC AGGACCAGCG GCGCGACGAG 
GAGAAGATCT ACCACAAGAT GAGCATTTCG GAGCTGCAGG CTCTGGCGCC CTCCATGGAC 
TGGCTTGAGT TCCTGTCTTT CTTGCTGTCA CCATTGGAGT TGAGTGACTC TGAGCCTGTG 
GTGGTGTATG GGATGGATTA TTTGCAGCAG GTGTCAGAGC TCATCAACCG CACGGAACCA 
AGCATCCTGA ACAATTACCT GATCTGGAAC CTGGTGCAAA AGACAACCTC AAGCCTGGAC 
CGACGCTTTG AGTCTGCACA AGAGAAGCTG CTGGAGACCC TCTATGGCAC TAAGAAGTCC 
TGTGTGCCGA GGTGGCAGAC CTGCATCTCC AACACGGATG ACGCCCTTGG CTTTGCTTTG 
GGGTCCCTCT TCGTGAAGGC CACGTTTGAC CGGCAAAGCA AAGAAATTGC AGAGGGGATG 
ATCAGCGAAA TCCGGACCGC ATTTGAGGAG GCCCTGGGAC AGCTGGTTTG GATGGATGAG 
AAGACCCGCC AGGCAGCCAA GGAGAAAGCA GATGCCATCT ATGATATGAT TGGTTTCCCA 
GACTTTATCC TGGAGCCCAA AGAGCTGGAT GATGTTTATG ACGGGTACGA AATTTCTGAA 
GATTCTTTCT TCCAAAACAT GTTGAATTTG TACAACTTCT CTGCCAAGGT TATGGCTGAC 
CAGCTCCGCA AGCCTCCCAG CCGAGACCAG TGGAGCATGA CCCCCCAGAC AGTGAATGCC 
TACTACCTTC CAACTAAGAA TGAGATCGTC TTCCCCGCTG GCATCCTGCA GGCCCCCTTC 
TATGCCCGCA ACCACCCCAA GGCCCTGAAC TTCGGTGGCA TCGGTGTGGT CATGGGCCAT 
GAGTTGACGC ATGCCTTTGA TGACCAAGGG CGCGAGTATG ACAAAGAAGG GAACCTGCGG 
CCCTGGTGGC AGAATGAGTC CCTGGCAGCC TTCCGGAACC ACACGGCCTG CATGGAGGAA 
CAGTACAATC AATACCAGGT CAATGGGGAG AGGCTCAACG GCCGCCAGAC GCTGGGGGAG 
AACATTGCTG ACAACGGGGG GCTGAAGGCT GCCTACAATG CTTACAAAGC ATGGCTGAGA 
AAGCATGGGG AGGAGCAGCA ACTGCCAGCC GTGGGGCTCA CCAACCACCA GCTCTTCTTC 
GTGGGATTTG CCCAGGTGTG GTGCTCGGTC CGCACACCAG AGAGCTCTCA CGAGGGGCTG 
GTGACCGACC CCCACAGCCC TGCCCGCTTC CGCGTGCTGG GCACTCTCTC CAACTCCCGT 
GACTTCCTGC GGCACTTCGG CTGCCCTGTC GGCTCCCCCA TGAACCCAGG GCAGCTGTGT 
GAGGTGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055508.3
CDS	39..2690
Translation	MASPGAGRAPPELPERNCGYREVEYWDQRYQGAADSAPYDWFGDFSSFRALLEPELRPEDRILVLGCGNSALSYELFLGGFPNVTSVDYSSVVVAAMQARHAHVPQLRWETMDVRKLDFPSASFDVVLEKGTLDALLAGERDPWTVSSEGVHTVDQVLSEVGFQKGTRQLLGSRTQLELVLAGASLLLAALLLGCLVALGVQYHRDPSHSTCLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAEQKTQRFYLSCLQVERIEELGAQPLRDLIEKIGGWNITGPWDQDNFMEVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGERLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW

Target ORF information:

RefSeq Version	NM_014693.3
Organism	Homo sapiens(human)
Definition	Homo sapiens EEF1AKMT4-ECE2 readthrough (EEF1AKMT4-ECE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014693.3

ORF Insert Sequence:

ATGGCCTCTC CAGGGGCAGG TAGGGCGCCT CCGGAGTTAC CGGAGCGGAA CTGCGGGTAC 
CGCGAAGTCG AGTACTGGGA TCAGCGCTAC CAAGGCGCAG CCGATTCTGC CCCCTACGAT 
TGGTTCGGGG ACTTCTCCTC CTTCCGTGCC CTCCTAGAGC CGGAGCTGCG GCCCGAGGAC 
CGTATCCTTG TGCTAGGTTG CGGGAACAGT GCCCTGAGCT ACGAGCTGTT CCTCGGAGGC 
TTCCCTAATG TGACCAGTGT GGACTACTCA TCAGTCGTGG TGGCTGCCAT GCAGGCTCGC 
CATGCCCATG TGCCGCAGCT GCGCTGGGAG ACCATGGATG TGCGGAAGCT GGACTTCCCC 
AGTGCTTCTT TTGATGTGGT GCTCGAGAAG GGCACGCTGG ATGCCCTGCT GGCTGGGGAA 
CGAGATCCCT GGACCGTGTC CTCTGAAGGT GTCCACACTG TGGACCAGGT GTTGAGTGAG 
GTGGGATTCC AGAAGGGGAC AAGACAGCTG TTAGGCTCAC GCACGCAGCT GGAGCTGGTC 
TTAGCAGGTG CCTCTCTACT GCTGGCTGCA CTGCTTCTGG GCTGCCTTGT GGCCCTAGGG 
GTCCAGTACC ACAGAGACCC ATCCCACAGC ACCTGCCTTA CAGAGGCCTG CATTCGAGTG 
GCTGGAAAAA TCCTGGAGTC CCTGGACCGA GGGGTGAGCC CCTGTGAGGA CTTTTACCAG 
TTCTCCTGTG GGGGCTGGAT TCGGAGGAAC CCCCTGCCCG ATGGGCGTTC TCGCTGGAAC 
ACCTTCAACA GCCTCTGGGA CCAAAACCAG GCCATACTGA AGCACCTGCT TGAAAACACC 
ACCTTCAACT CCAGCAGTGA AGCTGAGCAG AAGACACAGC GCTTCTACCT ATCTTGCCTA 
CAGGTGGAGC GCATTGAGGA GCTGGGAGCC CAGCCACTGA GAGACCTCAT TGAGAAGATT 
GGTGGTTGGA ACATTACGGG GCCCTGGGAC CAGGACAACT TTATGGAGGT GTTGAAGGCA 
GTAGCAGGGA CCTACAGGGC CACCCCATTC TTCACCGTCT ACATCAGTGC CGACTCTAAG 
AGTTCCAACA GCAATGTTAT CCAGGTGGAC CAGTCTGGGC TCTTTCTGCC CTCTCGGGAT 
TACTACTTAA ACAGAACTGC CAATGAGAAA GTGCTCACTG CCTATCTGGA TTACATGGAG 
GAACTGGGGA TGCTGCTGGG TGGGCGGCCC ACCTCCACGA GGGAGCAGAT GCAGCAGGTG 
CTGGAGTTGG AGATACAGCT GGCCAACATC ACAGTGCCCC AGGACCAGCG GCGCGACGAG 
GAGAAGATCT ACCACAAGAT GAGCATTTCG GAGCTGCAGG CTCTGGCGCC CTCCATGGAC 
TGGCTTGAGT TCCTGTCTTT CTTGCTGTCA CCATTGGAGT TGAGTGACTC TGAGCCTGTG 
GTGGTGTATG GGATGGATTA TTTGCAGCAG GTGTCAGAGC TCATCAACCG CACGGAACCA 
AGCATCCTGA ACAATTACCT GATCTGGAAC CTGGTGCAAA AGACAACCTC AAGCCTGGAC 
CGACGCTTTG AGTCTGCACA AGAGAAGCTG CTGGAGACCC TCTATGGCAC TAAGAAGTCC 
TGTGTGCCGA GGTGGCAGAC CTGCATCTCC AACACGGATG ACGCCCTTGG CTTTGCTTTG 
GGGTCCCTCT TCGTGAAGGC CACGTTTGAC CGGCAAAGCA AAGAAATTGC AGAGGGGATG 
ATCAGCGAAA TCCGGACCGC ATTTGAGGAG GCCCTGGGAC AGCTGGTTTG GATGGATGAG 
AAGACCCGCC AGGCAGCCAA GGAGAAAGCA GATGCCATCT ATGATATGAT TGGTTTCCCA 
GACTTTATCC TGGAGCCCAA AGAGCTGGAT GATGTTTATG ACGGGTACGA AATTTCTGAA 
GATTCTTTCT TCCAAAACAT GTTGAATTTG TACAACTTCT CTGCCAAGGT TATGGCTGAC 
CAGCTCCGCA AGCCTCCCAG CCGAGACCAG TGGAGCATGA CCCCCCAGAC AGTGAATGCC 
TACTACCTTC CAACTAAGAA TGAGATCGTC TTCCCCGCTG GCATCCTGCA GGCCCCCTTC 
TATGCCCGCA ACCACCCCAA GGCCCTGAAC TTCGGTGGCA TCGGTGTGGT CATGGGCCAT 
GAGTTGACGC ATGCCTTTGA TGACCAAGGG CGCGAGTATG ACAAAGAAGG GAACCTGCGG 
CCCTGGTGGC AGAATGAGTC CCTGGCAGCC TTCCGGAACC ACACGGCCTG CATGGAGGAA 
CAGTACAATC AATACCAGGT CAATGGGGAG AGGCTCAACG GCCGCCAGAC GCTGGGGGAG 
AACATTGCTG ACAACGGGGG GCTGAAGGCT GCCTACAATG CTTACAAAGC ATGGCTGAGA 
AAGCATGGGG AGGAGCAGCA ACTGCCAGCC GTGGGGCTCA CCAACCACCA GCTCTTCTTC 
GTGGGATTTG CCCAGGTGTG GTGCTCGGTC CGCACACCAG AGAGCTCTCA CGAGGGGCTG 
GTGACCGACC CCCACAGCCC TGCCCGCTTC CGCGTGCTGG GCACTCTCTC CAACTCCCGT 
GACTTCCTGC GGCACTTCGG CTGCCCTGTC GGCTCCCCCA TGAACCCAGG GCAGCTGTGT 
GAGGTGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Methylation of human eukaryotic elongation factor alpha (eEF1A) by a member of a novel protein lysine methyltransferase family modulates mRNA translation.
Nucleic acids research45(14)8239-8254(2017 Aug)
Jakobsson ME,Malecki J,Nilges BS,Moen A,Leidel SA,Falnes P?

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Genome research13(10)2265-70(2003 Oct)
Clark HF,Gurney AL,Abaya E,Baker K,Baldwin D,Brush J,Chen J,Chow B,Chui C,Crowley C,Currell B,Deuel B,Dowd P,Eaton D,Foster J,Grimaldi C,Gu Q,Hass PE,Heldens S,Huang A,Kim HS,Klimowski L,Jin Y,Johnson S,Lee J,Lewis L,Liao D,Mark M,Robbie E,Sanchez C,Schoenfeld J,Seshagiri S,Simmons L,Singh J,Smith V,Stinson J,Vagts A,Vandlen R,Watanabe C,Wieand D,Woods K,Xie MH,Yansura D,Yi S,Yu G,Yuan J,Zhang M,Zhang Z,Goddard A,Wood WI,Godowski P,Gray A

Human endothelin converting enzyme-2 (ECE2): characterization of mRNA species and chromosomal localization.
Biochimica et biophysica acta1522(1)46-52(2001 Nov)
Lorenzo MN,Khan RY,Wang Y,Tai SC,Chan GC,Cheung AH,Marsden PA

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EEF1AKMT4-ECE2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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