pfdn1 cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder)

Gene
Clones
gRNAs

Summary

Gene Symbol	pfdn1
Entrez Gene ID	102289202
Full Name	prefoldin subunit 1
Gene Type	protein-coding
Organism	Haplochromis burtoni(Burton's mouthbrooder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014331167.1	XP_014186653.1	prefoldin subunit 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comparative transcriptomics of Eastern African cichlid fishes shows signs of positive selection and a large contribution of untranslated regions to genetic diversity.
Baldo L, Santos ME, Salzburger W
Genome biology and evolution3443-55(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pfdn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pfdn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHa36169	XM_014331167.1 Latest version!	Haplochromis burtoni prefoldin subunit 1 (pfdn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pfdn1 ( XM_014331167.1 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_014186653.1 Haplochromis burtoni prefoldin subunit 1 (pfdn1), mRNA.

Price & Availability↑

CloneID	OHa36169
Clone ID Related Accession (Same CDS sequence)	XM_014331167.1
Accession Version	XM_014331167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	prefoldin subunit 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005181036.1) and transcript sequence (JL479066.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 94 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCGGCGC CCGTAGATCT GGAGCTGAAA AAGGCCTTCT CCGAGCTGCA GGTGAAGATG 
ATCGACACGC AGCAGAAAGT CAAGATGGCC GATCTGCAGA TCGATCAGCT GACGCGCGTT 
CAGAAGCACG CCAAACTGAC GCACGCCGAG ATCGCCACGC TGCCCGACAA CACGCGACTC 
TATGAGGGTG TGGGGCGCAT GTTCATCCTG CAGTCCAAGG AGGAGATCAA CAACCTGCTG 
ACAGACAAAC AGAAGACGGC TGACGAGAAG ATCAAAGAGT TGGAGCAGAG GAAGGTGTAC 
CTTGAACGCA GCGTGAAGGA AGCTGAGGAC AACATAAGAG AGATGCTGCT GTCCAGGAGA 
GCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014186653.1
CDS	83..451
Translation	MAAPVDLELKKAFSELQVKMIDTQQKVKMADLQIDQLTRVQKHAKLTHAEIATLPDNTRLYEGVGRMFILQSKEEINNLLTDKQKTADEKIKELEQRKVYLERSVKEAEDNIREMLLSRRAH

Target ORF information:

RefSeq Version	XM_014331167.1
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni prefoldin subunit 1 (pfdn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014331167.1

ORF Insert Sequence:

ATGGCGGCGC CCGTAGATCT GGAGCTGAAA AAGGCCTTCT CCGAGCTGCA GGTGAAGATG 
ATCGACACGC AGCAGAAAGT CAAGATGGCC GATCTGCAGA TCGATCAGCT GACGCGCGTT 
CAGAAGCACG CCAAACTGAC GCACGCCGAG ATCGCCACGC TGCCCGACAA CACGCGACTC 
TATGAGGGTG TGGGGCGCAT GTTCATCCTG CAGTCCAAGG AGGAGATCAA CAACCTGCTG 
ACAGACAAAC AGAAGACGGC TGACGAGAAG ATCAAAGAGT TGGAGCAGAG GAAGGTGTAC 
CTTGAACGCA GCGTGAAGGA AGCTGAGGAC AACATAAGAG AGATGCTGCT GTCCAGGAGA 
GCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative transcriptomics of Eastern African cichlid fishes shows signs of positive selection and a large contribution of untranslated regions to genetic diversity.
Genome biology and evolution3443-55(2011)
Baldo L,Santos ME,Salzburger W