LIG3 cDNA ORF clone, Geospiza fortis(medium ground-finch)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIG3
Entrez Gene ID	102037223
Full Name	DNA ligase 3
Gene Type	protein-coding
Organism	Geospiza fortis(medium ground-finch)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031060676.1	XP_030916536.1	LOW QUALITY PROTEIN: DNA ligase 3
XM_005425401.1	XP_005425458.1	LOW QUALITY PROTEIN: DNA ligase 3

Pathways from BioSystems
Gene Ontology

The following LIG3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIG3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe09588	XM_005425401.1 Latest version!	Geospiza fortis ligase III, DNA, ATP-dependent (LIG3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OGe94207	XM_031060676.1 Latest version!	Geospiza fortis DNA ligase 3 (LIG3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIG3 ( XM_005425401.1 ) cDNA ORF clone, Geospiza fortis(medium ground-finch) -> XP_005425458.1 Geospiza fortis ligase III, DNA, ATP-dependent (LIG3), mRNA.

Price & Availability↑

CloneID	OGe09588
Clone ID Related Accession (Same CDS sequence)	XM_005425401.1
Accession Version	XM_005425401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-23
Organism	Geospiza fortis(medium ground-finch)
Product	LOW QUALITY PROTEIN: DNA ligase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005054387.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Geospiza fortis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 4 indels ##RefSeq-Attributes-END##

ATGGCGGAGC AGCGGTACTG CGTGGACTAT GCCAAGCGGG GCACCGCGGG CTGCAAGAAG 
TGCAAGGAGA AGATCGTGAA GGGGATGGTG CGCATTGGGA AGATTGTTCC CAATCCTTTC 
ACCGAGTCTG GAGGGGACAT GAAGGAGTGG TACCATGTCA AGTGCATCTT TGAGAAGCTG 
GACAAGGCCC GGGCCACCAC TAAGAAAATT GAAGACATCA CAGACTTGGA GGGGTGGGAA 
GAGCTGCAGG ATGAGGAGAA GGAAACAATC AACAAGTACA TCTCAGAAGC CAATTCCAAG 
ACTGGAGGTA CCCCAAAGAA AAAGGTGATA GTGCAGGCAA AACTCACTGC CACAGGACAG 
CTGACCACAA AAGATCCACC GAAGAAATTC TCTGGATTCT CTGCCAAGCC AAAGAATTCA 
GAAGATTCTC CTGCAAAGTG TGCCCAGAAG CCGAGCCTGT CTGCAGCCCG GTGTGACCCC 
CAGCACAAGG ACTGCCTGCT GCGCGAGTTC CGCAAGCTCT GCGCCATGGT GGCTGAGAAA 
CCAAGCTACA ACGTCAAAAC CCAGATCATC CAGGACTTCC TCAGGAAGGG ATCTGCAGGA 
GATGGTTTTC ATGGTGATGT ATACCTGACC ATTAAGATGC TGTTACCAGG TGTCATTAAA 
ATAGTTTACA ACTTGAATGA TAAGCAGATT GTAAAGCTGT TCANNNNNNN NNNTAGCAGG 
ATTTTTAACT GCAACCAGGA AGAAATGATC CGGGACCTGG AGCAGGGAGA TGTTTCTGAG 
ACCATCCGGA TTTTCTTCGA GCAGAGCAAG ACCTGTCCTC CAGCAGCCAA GAGCCTCCTG 
ACCATCCAGG AGGTGGATGA GTTCCTCATG CAGCTGTCCA AGCTCACCAA GGAGGATGAC 
CAGCAGAGTG TCCTGCAGCA GATCAGTCGC AGGTGTACAG GCAATGACCT GAAGTGCATC 
ATCAGGCTAA TTAAGCATGA CTTGAAAATG AATGCTGGAG CAAAGCATGT GTTGGACGCT 
CTGGATCCCA ACGCCTACGA GGCGTTCAAG GCCTCGAGGA ACCTGCAGGA CGTGGTGGAG 
AGGGTGCTGC AGAACCAGCA GGAGGCAGAG AAGGTGCCAG GGCTGAAGAA GACCCTGAGT 
GTGCAGGCCT CACTCATGAC CCCAGTGCAG CCCATGCTGG CTGAGGCCTG CAAGTCCATC 
GAGTACGCCA TGAAGAAGTG TCCCAATGGG ATGTACGCCG AGATCAAGTA CGACGGCGAG 
CGCGTGCAGG TGCACAAGAA CGGGGATCAC TTCAGCTACT TCAGNNNNNN NNNNNGGAGC 
CTCCAGCCTG TGCTCCCCCA CAAAGTAGCC CATTTCAAGG ATTTCATCCC CCAGGCTTTC 
CCTGGTGGGC AAAGTATGAT TCTGGATTCA GAAGTTCTCC TGATTGACAA TAAAACTGGC 
AAGCCACTTC CTTTTGGGAC TCTTGGTGTG CACAAGAAAG CTGCTTTCCA GGATGCCAAC 
GTTTGCCTGT TTGTGTTTGA CTGCATCTAC TTCAATGACA TCAGCCTGAT GGACAGGCCC 
CTCTGTGAAC GGCGCAAGTT CCTCCACGAC AACATGGTGG AGATCCCCAA CNAGGATCCT 
GTTCTCAGAG ATGAAACATG TCACAAAAGC TTCAGATCTG GCAGACATGA TCACCGGGTC 
ATCCGGGAGG GGCTGGAAGG GCTGGTGCTG AAGGACATAA AGGGTAATTA TGAGCCAGGG 
AAACGCCACT GGCTGAAGGT GAAGAAGGAT TATCTCAACG AGGGGGCAAT GGCTGACACT 
GCTGACCTGG TGGTGCTGGG GGCTTTCTAT GGACAAGGCA GTAAAGGTGG GATGATGTCC 
ATCTTCCTCA TGGGCTGCTA CGACCCCAAG AGTGAGAAGT GGTGCACGGT GACCAAGTGC 
TCTGGTGGCC ACGATGACGC CACCCTGGCA CGTCTGCAGA CAGAGCTGGA CATGGTGAAG 
ATCAGCAAGG ATCCTAGTAA AATTCCAAGG TGGCTAAAAA TTAACAAAAT CTACTACCCA 
GACTTCATTG TCCCAGATCC AAAGAAAGCC CCGGTGTGGG AGATCACGGG GGCCGAGTTC 
TCCAAGGCCG AGGCGCACAC GGCTGACGGG ATCTCCATCC GCTTCCCGCG CTGCACCCGC 
ATCCGCGATG ACAAGGACTG GCAGACAGCC ACCAACCTGC AGCAGCTCAA GGAGCTGTAC 
CAGCTCTCCA AGGAGAAGGC TGATTTCAGT GTTACAGCTG GGGAGGAAGA TGAGTCCACA 
GCTGGCAGCA GTGGAGAGAA TGATGGGAAT TCCAGGTCTT CCACACCTCA CAGCAGTATT 
AAGAGTCCCC CAAGCAAGTC ACCTGCAAAA GCCCAGAAGG CAGAAGACAG CAAAGGAATC 
GTTGGATCCC CTCAAAAATC CGAGGAGAGA CGAGGGGAGA AAAGAAAAGC GTCTGAGATG 
GAGGACAATG GAAAAAAGCC CCTGCTGGAC ATTTTCACGG GGGTGAAGCT GTACCTGGCG 
CCCTCGGTGC CGGACTTTGC GCGGATCCGC CGCTACTTCA TCGCCTTCGA CGGGGACCTG 
GTGGAGGAGT TCGACACGGC CTCGGCCACC CACGTGCTTG GGGACATTGA TGACAACCCC 
GGGGCGCAGC GTGTGACTCC CAGGTGGATC TGGGAGTGCA TCCGGAAGCG CAGGCTGGTG 
GCGCCGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005425458.1
CDS	8..2719
Translation	MAEQRYCVDYAKRGTAGCKKCKEKIVKGMVRIGKIVPNPFTESGGDMKEWYHVKCIFEKLDKARATTKKIEDITDLEGWEELQDEEKETINKYISEANSKTGGTPKKKVIVQAKLTATGQLTTKDPPKKFSGFSAKPKNSEDSPAKCAQKPSLSAARCDPQHKDCLLREFRKLCAMVAEKPSYNVKTQIIQDFLRKGSAGDGFHGDVYLTIKMLLPGVIKIVYNLNDKQIVKLFnullnullnullnullSRIFNCNQEEMIRDLEQGDVSETIRIFFEQSKTCPPAAKSLLTIQEVDEFLMQLSKLTKEDDQQSVLQQISRRCTGNDLKCIIRLIKHDLKMNAGAKHVLDALDPNAYEAFKASRNLQDVVERVLQNQQEAEKVPGLKKTLSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMYAEIKYDGERVQVHKNGDHFSYFnullnullnullnullnullSLQPVLPHKVAHFKDFIPQAFPGGQSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDISLMDRPLCERRKFLHDNMVEIPNnullDPVLRDETCHKSFRSGRHDHRVIREGLEGLVLKDIKGNYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPKSEKWCTVTKCSGGHDDATLARLQTELDMVKISKDPSKIPRWLKINKIYYPDFIVPDPKKAPVWEITGAEFSKAEAHTADGISIRFPRCTRIRDDKDWQTATNLQQLKELYQLSKEKADFSVTAGEEDESTAGSSGENDGNSRSSTPHSSIKSPPSKSPAKAQKAEDSKGIVGSPQKSEERRGEKRKASEMEDNGKKPLLDIFTGVKLYLAPSVPDFARIRRYFIAFDGDLVEEFDTASATHVLGDIDDNPGAQRVTPRWIWECIRKRRLVAPC

Target ORF information:

RefSeq Version	XM_005425401.1
Organism	Geospiza fortis(medium ground-finch)
Definition	Geospiza fortis ligase III, DNA, ATP-dependent (LIG3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005425401.1

ORF Insert Sequence:

ATGGCGGAGC AGCGGTACTG CGTGGACTAT GCCAAGCGGG GCACCGCGGG CTGCAAGAAG 
TGCAAGGAGA AGATCGTGAA GGGGATGGTG CGCATTGGGA AGATTGTTCC CAATCCTTTC 
ACCGAGTCTG GAGGGGACAT GAAGGAGTGG TACCATGTCA AGTGCATCTT TGAGAAGCTG 
GACAAGGCCC GGGCCACCAC TAAGAAAATT GAAGACATCA CAGACTTGGA GGGGTGGGAA 
GAGCTGCAGG ATGAGGAGAA GGAAACAATC AACAAGTACA TCTCAGAAGC CAATTCCAAG 
ACTGGAGGTA CCCCAAAGAA AAAGGTGATA GTGCAGGCAA AACTCACTGC CACAGGACAG 
CTGACCACAA AAGATCCACC GAAGAAATTC TCTGGATTCT CTGCCAAGCC AAAGAATTCA 
GAAGATTCTC CTGCAAAGTG TGCCCAGAAG CCGAGCCTGT CTGCAGCCCG GTGTGACCCC 
CAGCACAAGG ACTGCCTGCT GCGCGAGTTC CGCAAGCTCT GCGCCATGGT GGCTGAGAAA 
CCAAGCTACA ACGTCAAAAC CCAGATCATC CAGGACTTCC TCAGGAAGGG ATCTGCAGGA 
GATGGTTTTC ATGGTGATGT ATACCTGACC ATTAAGATGC TGTTACCAGG TGTCATTAAA 
ATAGTTTACA ACTTGAATGA TAAGCAGATT GTAAAGCTGT TCANNNNNNN NNNTAGCAGG 
ATTTTTAACT GCAACCAGGA AGAAATGATC CGGGACCTGG AGCAGGGAGA TGTTTCTGAG 
ACCATCCGGA TTTTCTTCGA GCAGAGCAAG ACCTGTCCTC CAGCAGCCAA GAGCCTCCTG 
ACCATCCAGG AGGTGGATGA GTTCCTCATG CAGCTGTCCA AGCTCACCAA GGAGGATGAC 
CAGCAGAGTG TCCTGCAGCA GATCAGTCGC AGGTGTACAG GCAATGACCT GAAGTGCATC 
ATCAGGCTAA TTAAGCATGA CTTGAAAATG AATGCTGGAG CAAAGCATGT GTTGGACGCT 
CTGGATCCCA ACGCCTACGA GGCGTTCAAG GCCTCGAGGA ACCTGCAGGA CGTGGTGGAG 
AGGGTGCTGC AGAACCAGCA GGAGGCAGAG AAGGTGCCAG GGCTGAAGAA GACCCTGAGT 
GTGCAGGCCT CACTCATGAC CCCAGTGCAG CCCATGCTGG CTGAGGCCTG CAAGTCCATC 
GAGTACGCCA TGAAGAAGTG TCCCAATGGG ATGTACGCCG AGATCAAGTA CGACGGCGAG 
CGCGTGCAGG TGCACAAGAA CGGGGATCAC TTCAGCTACT TCAGNNNNNN NNNNNGGAGC 
CTCCAGCCTG TGCTCCCCCA CAAAGTAGCC CATTTCAAGG ATTTCATCCC CCAGGCTTTC 
CCTGGTGGGC AAAGTATGAT TCTGGATTCA GAAGTTCTCC TGATTGACAA TAAAACTGGC 
AAGCCACTTC CTTTTGGGAC TCTTGGTGTG CACAAGAAAG CTGCTTTCCA GGATGCCAAC 
GTTTGCCTGT TTGTGTTTGA CTGCATCTAC TTCAATGACA TCAGCCTGAT GGACAGGCCC 
CTCTGTGAAC GGCGCAAGTT CCTCCACGAC AACATGGTGG AGATCCCCAA CNAGGATCCT 
GTTCTCAGAG ATGAAACATG TCACAAAAGC TTCAGATCTG GCAGACATGA TCACCGGGTC 
ATCCGGGAGG GGCTGGAAGG GCTGGTGCTG AAGGACATAA AGGGTAATTA TGAGCCAGGG 
AAACGCCACT GGCTGAAGGT GAAGAAGGAT TATCTCAACG AGGGGGCAAT GGCTGACACT 
GCTGACCTGG TGGTGCTGGG GGCTTTCTAT GGACAAGGCA GTAAAGGTGG GATGATGTCC 
ATCTTCCTCA TGGGCTGCTA CGACCCCAAG AGTGAGAAGT GGTGCACGGT GACCAAGTGC 
TCTGGTGGCC ACGATGACGC CACCCTGGCA CGTCTGCAGA CAGAGCTGGA CATGGTGAAG 
ATCAGCAAGG ATCCTAGTAA AATTCCAAGG TGGCTAAAAA TTAACAAAAT CTACTACCCA 
GACTTCATTG TCCCAGATCC AAAGAAAGCC CCGGTGTGGG AGATCACGGG GGCCGAGTTC 
TCCAAGGCCG AGGCGCACAC GGCTGACGGG ATCTCCATCC GCTTCCCGCG CTGCACCCGC 
ATCCGCGATG ACAAGGACTG GCAGACAGCC ACCAACCTGC AGCAGCTCAA GGAGCTGTAC 
CAGCTCTCCA AGGAGAAGGC TGATTTCAGT GTTACAGCTG GGGAGGAAGA TGAGTCCACA 
GCTGGCAGCA GTGGAGAGAA TGATGGGAAT TCCAGGTCTT CCACACCTCA CAGCAGTATT 
AAGAGTCCCC CAAGCAAGTC ACCTGCAAAA GCCCAGAAGG CAGAAGACAG CAAAGGAATC 
GTTGGATCCC CTCAAAAATC CGAGGAGAGA CGAGGGGAGA AAAGAAAAGC GTCTGAGATG 
GAGGACAATG GAAAAAAGCC CCTGCTGGAC ATTTTCACGG GGGTGAAGCT GTACCTGGCG 
CCCTCGGTGC CGGACTTTGC GCGGATCCGC CGCTACTTCA TCGCCTTCGA CGGGGACCTG 
GTGGAGGAGT TCGACACGGC CTCGGCCACC CACGTGCTTG GGGACATTGA TGACAACCCC 
GGGGCGCAGC GTGTGACTCC CAGGTGGATC TGGGAGTGCA TCCGGAAGCG CAGGCTGGTG 
GCGCCGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG3 ( XM_031060676.1 ) cDNA ORF clone, Geospiza fortis(medium ground-finch) -> XP_030916536.1 Geospiza fortis DNA ligase 3 (LIG3), mRNA.

Price & Availability↑

CloneID	OGe94207
Clone ID Related Accession (Same CDS sequence)	XM_031060676.1
Accession Version	XM_031060676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Geospiza fortis(medium ground-finch)
Product	LOW QUALITY PROTEIN: DNA ligase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005054387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Geospiza fortis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGCCCACGG GCGGCAGGAC GCTGTCACAC GCTGCCCGCG CTCTCGGCAG GGGCACAGCG 
CTCTTCTGCC CTCGCCCACA GCTCCCCGTG CCCAGGCCGT TGTCCCGCCG TGCCGGAGCC 
AGCCGCGGGT GCTGGTGCCA GCTGCTGTTC CGGCCTGTCC CTGTGCCATC CCCGCAGCCG 
GCGCTTCCCC TGTCCCTGCT GTTTGGCAGC CTGCCGCGCC CAGCCTGCGC GGCTGCCGGG 
GACATGGCGG AGCAGCGGTA CTGCGTGGAC TATGCCAAGC GGGGCACCGC GGGCTGCAAG 
AAGTGCAAGG AGAAGATCGT GAAGGGGATG GTGCGCATTG GGAAGATTGT TCCCAATCCT 
TTCACCGAGT CTGGAGGGGA CATGAAGGAG TGGTACCATG TCAAGTGCAT CTTTGAGAAG 
CTGGACAAGG CCCGGGCCAC CACTAAGAAA ATTGAAGACA TCACAGACTT GGAGGGGTGG 
GAAGAGCTGC AGGATGAGGA GAAGGAAACA ATCAACAAGT ACATCTCAGA AGCCAATTCC 
AAGACTGGAG GTACCCCAAA GAAAAAGGTG ATAGTGCAGG CAAAACTCAC TGCCACAGGA 
CAGCTGACCA CAAAAGATCC ACCGAAGAAA TTCTCTGGAT TCTCTGCCAA GCCAAAGAAT 
TCAGAAGATT CTCCTGCAAA GTGTGCCCAG AAGCCGAGCC TGTCTGCAGC CCGGTGTGAC 
CCCCAGCACA AGGACTGCCT GCTGCGCGAG TTCCGCAAGC TCTGCGCCAT GGTGGCTGAG 
AAACCAAGCT ACAACGTCAA AACCCAGATC ATCCAGGACT TCCTCAGGAA GGGATCTGCA 
GGAGATGGTT TTCATGGTGA TGTATACCTG ACCATTAAGA TGCTGTTACC AGGTGTCATT 
AAAATAGTTT ACAACTTGAA TGATAAGCAG ATTGTAAAGC TGTTCANNNN NNNNNNTAGC 
AGGATTTTTA ACTGCAACCA GGAAGAAATG ATCCGGGACC TGGAGCAGGG AGATGTTTCT 
GAGACCATCC GGATTTTCTT CGAGCAGAGC AAGACCTGTC CTCCAGCAGC CAAGAGCCTC 
CTGACCATCC AGGAGGTGGA TGAGTTCCTC ATGCAGCTGT CCAAGCTCAC CAAGGAGGAT 
GACCAGCAGA GTGTCCTGCA GCAGATCAGT CGCAGGTGTA CAGGCAATGA CCTGAAGTGC 
ATCATCAGGC TAATTAAGCA TGACTTGAAA ATGAATGCTG GAGCAAAGCA TGTGTTGGAC 
GCTCTGGATC CCAACGCCTA CGAGGCGTTC AAGGCCTCGA GGAACCTGCA GGACGTGGTG 
GAGAGGGTGC TGCAGAACCA GCAGGAGGCA GAGAAGGTGC CAGGGCTGAA GAAGACCCTG 
AGTGTGCAGG CCTCACTCAT GACCCCAGTG CAGCCCATGC TGGCTGAGGC CTGCAAGTCC 
ATCGAGTACG CCATGAAGAA GTGTCCCAAT GGGATGTACG CCGAGATCAA GTACGACGGC 
GAGCGCGTGC AGGTGCACAA GAACGGGGAT CACTTCAGCT ACTTCAGNNN NNNNNNNNGG 
AGCCTCCAGC CTGTGCTCCC CCACAAAGTA GCCCATTTCA AGGATTTCAT CCCCCAGGCT 
TTCCCTGGTG GGCAAAGTAT GATTCTGGAT TCAGAAGTTC TCCTGATTGA CAATAAAACT 
GGCAAGCCAC TTCCTTTTGG GACTCTTGGT GTGCACAAGA AAGCTGCTTT CCAGGATGCC 
AACGTTTGCC TGTTTGTGTT TGACTGCATC TACTTCAATG ACATCAGCCT GATGGACAGG 
CCCCTCTGTG AACGGCGCAA GTTCCTCCAC GACAACATGG TGGAGATCCC CAACAGGATC 
CTGTTCTCAG AGATGAAACA TGTCACAAAA GCTTCAGATC TGGCAGACAT GATCACCCGG 
GTCATCCGGG AGGGGCTGGA AGGGCTGGTG CTGAAGGACA TAAAGGGTAA TTATGAGCCA 
GGGAAACGCC ACTGGCTGAA GGTGAAGAAG GATTATCTCA ACGAGGGGGC AATGGCTGAC 
ACTGCTGACC TGGTGGTGCT GGGGGCTTTC TATGGACAAG GCAGTAAAGG TGGGATGATG 
TCCATCTTCC TCATGGGCTG CTACGACCCC AAGAGTGAGA AGTGGTGCAC GGTGACCAAG 
TGCTCTGGTG GCCACGATGA CGCCACCCTG GCACGTCTGC AGACAGAGCT GGACATGGTG 
AAGATCAGCA AGGATCCTAG TAAAATTCCA AGGTGGCTAA AAATTAACAA AATCTACTAC 
CCAGACTTCA TTGTCCCAGA TCCAAAGAAA GCCCCGGTGT GGGAGATCAC GGGGGCCGAG 
TTCTCCAAGG CCGAGGCGCA CACGGCTGAC GGGATCTCCA TCCGCTTCCC GCGCTGCACC 
CGCATCCGCG ATGACAAGGA CTGGCAGACA GCCACCAACC TGCAGCAGCT CAAGGAGCTG 
TACCAGCTCT CCAAGGAGAA GGCTGATTTC AGTGTTACAG CTGGGGAGGA AGATGAGTCC 
ACAGCTGGCA GCAGTGGAGA GAATGATGGG AATTCCAGGT CTTCCACACC TCACAGCAGT 
ATTAAGAGTC CCCCAAGCAA GTCACCTGCA AAAGCCCAGA AGGCAGAAGA CAGCAAAGGA 
ATCGTTGGAT CCCCTCAAAA ATCCGAGGAG AGACGAGGGG AGAAAAGAAA AGCGTCTGAG 
ATGGAGGACA ATGGAAAAAA GCAGCCCCTG CTGGACATTT TCACGGGGGT GAAGCTGTAC 
CTGGCGCCCT CGGTGCCGGA CTTTGCGCGG ATCCGCCGCT ACTTCATCGC CTTCGACGGG 
GACCTGGTGG AGGAGTTCGA CACGGCCTCG GCCACCCACG TGCTTGGGGA CATTGATGAC 
AACCCCGGGG CGCAGCGTGT GACTCCCAGG TGGATCTGGG AGTGCATCCG GAAGCGCAGG 
CTGGTGGCGC CGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030916536.1
CDS	22..2979
Translation	MPTGGRTLSHAARALGRGTALFCPRPQLPVPRPLSRRAGASRGCWCQLLFRPVPVPSPQPALPLSLLFGSLPRPACAAAGDMAEQRYCVDYAKRGTAGCKKCKEKIVKGMVRIGKIVPNPFTESGGDMKEWYHVKCIFEKLDKARATTKKIEDITDLEGWEELQDEEKETINKYISEANSKTGGTPKKKVIVQAKLTATGQLTTKDPPKKFSGFSAKPKNSEDSPAKCAQKPSLSAARCDPQHKDCLLREFRKLCAMVAEKPSYNVKTQIIQDFLRKGSAGDGFHGDVYLTIKMLLPGVIKIVYNLNDKQIVKLFnullnullnullnullSRIFNCNQEEMIRDLEQGDVSETIRIFFEQSKTCPPAAKSLLTIQEVDEFLMQLSKLTKEDDQQSVLQQISRRCTGNDLKCIIRLIKHDLKMNAGAKHVLDALDPNAYEAFKASRNLQDVVERVLQNQQEAEKVPGLKKTLSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMYAEIKYDGERVQVHKNGDHFSYFnullnullnullnullnullSLQPVLPHKVAHFKDFIPQAFPGGQSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDISLMDRPLCERRKFLHDNMVEIPNRILFSEMKHVTKASDLADMITRVIREGLEGLVLKDIKGNYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPKSEKWCTVTKCSGGHDDATLARLQTELDMVKISKDPSKIPRWLKINKIYYPDFIVPDPKKAPVWEITGAEFSKAEAHTADGISIRFPRCTRIRDDKDWQTATNLQQLKELYQLSKEKADFSVTAGEEDESTAGSSGENDGNSRSSTPHSSIKSPPSKSPAKAQKAEDSKGIVGSPQKSEERRGEKRKASEMEDNGKKQPLLDIFTGVKLYLAPSVPDFARIRRYFIAFDGDLVEEFDTASATHVLGDIDDNPGAQRVTPRWIWECIRKRRLVAPC

Target ORF information:

RefSeq Version	XM_031060676.1
Organism	Geospiza fortis(medium ground-finch)
Definition	Geospiza fortis DNA ligase 3 (LIG3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031060676.1

ORF Insert Sequence:

ATGCCCACGG GCGGCAGGAC GCTGTCACAC GCTGCCCGCG CTCTCGGCAG GGGCACAGCG 
CTCTTCTGCC CTCGCCCACA GCTCCCCGTG CCCAGGCCGT TGTCCCGCCG TGCCGGAGCC 
AGCCGCGGGT GCTGGTGCCA GCTGCTGTTC CGGCCTGTCC CTGTGCCATC CCCGCAGCCG 
GCGCTTCCCC TGTCCCTGCT GTTTGGCAGC CTGCCGCGCC CAGCCTGCGC GGCTGCCGGG 
GACATGGCGG AGCAGCGGTA CTGCGTGGAC TATGCCAAGC GGGGCACCGC GGGCTGCAAG 
AAGTGCAAGG AGAAGATCGT GAAGGGGATG GTGCGCATTG GGAAGATTGT TCCCAATCCT 
TTCACCGAGT CTGGAGGGGA CATGAAGGAG TGGTACCATG TCAAGTGCAT CTTTGAGAAG 
CTGGACAAGG CCCGGGCCAC CACTAAGAAA ATTGAAGACA TCACAGACTT GGAGGGGTGG 
GAAGAGCTGC AGGATGAGGA GAAGGAAACA ATCAACAAGT ACATCTCAGA AGCCAATTCC 
AAGACTGGAG GTACCCCAAA GAAAAAGGTG ATAGTGCAGG CAAAACTCAC TGCCACAGGA 
CAGCTGACCA CAAAAGATCC ACCGAAGAAA TTCTCTGGAT TCTCTGCCAA GCCAAAGAAT 
TCAGAAGATT CTCCTGCAAA GTGTGCCCAG AAGCCGAGCC TGTCTGCAGC CCGGTGTGAC 
CCCCAGCACA AGGACTGCCT GCTGCGCGAG TTCCGCAAGC TCTGCGCCAT GGTGGCTGAG 
AAACCAAGCT ACAACGTCAA AACCCAGATC ATCCAGGACT TCCTCAGGAA GGGATCTGCA 
GGAGATGGTT TTCATGGTGA TGTATACCTG ACCATTAAGA TGCTGTTACC AGGTGTCATT 
AAAATAGTTT ACAACTTGAA TGATAAGCAG ATTGTAAAGC TGTTCANNNN NNNNNNTAGC 
AGGATTTTTA ACTGCAACCA GGAAGAAATG ATCCGGGACC TGGAGCAGGG AGATGTTTCT 
GAGACCATCC GGATTTTCTT CGAGCAGAGC AAGACCTGTC CTCCAGCAGC CAAGAGCCTC 
CTGACCATCC AGGAGGTGGA TGAGTTCCTC ATGCAGCTGT CCAAGCTCAC CAAGGAGGAT 
GACCAGCAGA GTGTCCTGCA GCAGATCAGT CGCAGGTGTA CAGGCAATGA CCTGAAGTGC 
ATCATCAGGC TAATTAAGCA TGACTTGAAA ATGAATGCTG GAGCAAAGCA TGTGTTGGAC 
GCTCTGGATC CCAACGCCTA CGAGGCGTTC AAGGCCTCGA GGAACCTGCA GGACGTGGTG 
GAGAGGGTGC TGCAGAACCA GCAGGAGGCA GAGAAGGTGC CAGGGCTGAA GAAGACCCTG 
AGTGTGCAGG CCTCACTCAT GACCCCAGTG CAGCCCATGC TGGCTGAGGC CTGCAAGTCC 
ATCGAGTACG CCATGAAGAA GTGTCCCAAT GGGATGTACG CCGAGATCAA GTACGACGGC 
GAGCGCGTGC AGGTGCACAA GAACGGGGAT CACTTCAGCT ACTTCAGNNN NNNNNNNNGG 
AGCCTCCAGC CTGTGCTCCC CCACAAAGTA GCCCATTTCA AGGATTTCAT CCCCCAGGCT 
TTCCCTGGTG GGCAAAGTAT GATTCTGGAT TCAGAAGTTC TCCTGATTGA CAATAAAACT 
GGCAAGCCAC TTCCTTTTGG GACTCTTGGT GTGCACAAGA AAGCTGCTTT CCAGGATGCC 
AACGTTTGCC TGTTTGTGTT TGACTGCATC TACTTCAATG ACATCAGCCT GATGGACAGG 
CCCCTCTGTG AACGGCGCAA GTTCCTCCAC GACAACATGG TGGAGATCCC CAACAGGATC 
CTGTTCTCAG AGATGAAACA TGTCACAAAA GCTTCAGATC TGGCAGACAT GATCACCCGG 
GTCATCCGGG AGGGGCTGGA AGGGCTGGTG CTGAAGGACA TAAAGGGTAA TTATGAGCCA 
GGGAAACGCC ACTGGCTGAA GGTGAAGAAG GATTATCTCA ACGAGGGGGC AATGGCTGAC 
ACTGCTGACC TGGTGGTGCT GGGGGCTTTC TATGGACAAG GCAGTAAAGG TGGGATGATG 
TCCATCTTCC TCATGGGCTG CTACGACCCC AAGAGTGAGA AGTGGTGCAC GGTGACCAAG 
TGCTCTGGTG GCCACGATGA CGCCACCCTG GCACGTCTGC AGACAGAGCT GGACATGGTG 
AAGATCAGCA AGGATCCTAG TAAAATTCCA AGGTGGCTAA AAATTAACAA AATCTACTAC 
CCAGACTTCA TTGTCCCAGA TCCAAAGAAA GCCCCGGTGT GGGAGATCAC GGGGGCCGAG 
TTCTCCAAGG CCGAGGCGCA CACGGCTGAC GGGATCTCCA TCCGCTTCCC GCGCTGCACC 
CGCATCCGCG ATGACAAGGA CTGGCAGACA GCCACCAACC TGCAGCAGCT CAAGGAGCTG 
TACCAGCTCT CCAAGGAGAA GGCTGATTTC AGTGTTACAG CTGGGGAGGA AGATGAGTCC 
ACAGCTGGCA GCAGTGGAGA GAATGATGGG AATTCCAGGT CTTCCACACC TCACAGCAGT 
ATTAAGAGTC CCCCAAGCAA GTCACCTGCA AAAGCCCAGA AGGCAGAAGA CAGCAAAGGA 
ATCGTTGGAT CCCCTCAAAA ATCCGAGGAG AGACGAGGGG AGAAAAGAAA AGCGTCTGAG 
ATGGAGGACA ATGGAAAAAA GCAGCCCCTG CTGGACATTT TCACGGGGGT GAAGCTGTAC 
CTGGCGCCCT CGGTGCCGGA CTTTGCGCGG ATCCGCCGCT ACTTCATCGC CTTCGACGGG 
GACCTGGTGG AGGAGTTCGA CACGGCCTCG GCCACCCACG TGCTTGGGGA CATTGATGAC 
AACCCCGGGG CGCAGCGTGT GACTCCCAGG TGGATCTGGG AGTGCATCCG GAAGCGCAGG 
CTGGTGGCGC CGTGCTAG