VEGFA cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	VEGFA
Entrez Gene ID	395909
Full Name	vascular endothelial growth factor A
Synonyms	VEGF
General protein information	Preferred Names vascular endothelial growth factor A Names vascular endothelial growth factor A VEGF-A VPF vascular permeability factor
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205042.2	NP_990373.1	vascular endothelial growth factor A isoform 1 precursor
NM_001110355.1	NP_001103825.1	vascular endothelial growth factor A isoform 2 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Vegfa	NP_114024.2
Gallus gallus (chicken)	VEGFA	NP_990373.1
Canis lupus familiaris (dog)	VEGFA	NP_001003175.2
Bos taurus (cattle)	VEGFA	NP_776641.1
Mus musculus (house mouse)	Vegfa	NP_001020421.2
Homo sapiens (human)	VEGFA	NP_003367.4
Danio rerio (zebrafish)	vegfab	NP_001038320.2

Gene Ontology
Bibliography

Related articles in PubMed

Avian leukosis virus subgroup J induces VEGF expression via NF-?B/PI3K-dependent IL-6 production.
Gao Y, Zhang Y, Yao Y, Guan X, Liu Y, Qi X, Wang Y, Liu C, Zhang Y, Gao H, Nair V, Wang X, Gao Y
Oncotarget7(49)80275-80287(2016 Dec)

Basic FGF or VEGF gene therapy corrects insufficiency in the intrinsic healing capacity of tendons.
Tang JB, Wu YF, Cao Y, Chen CH, Zhou YL, Avanessian B, Shimada M, Wang XT, Liu PY
Scientific reports620643(2016 Feb)

Chronic hypoxia and hyperoxia modifies morphology and VEGF concentration of the lungs of the developing chicken (Gallus gallus variant domesticus).
Lewallen MA, Burggren WW
Respiratory physiology & neurobiology21985-94(2015 Dec)

Exposure of chick embryos to cadmium changes the extra-embryonic vascular branching pattern and alters expression of VEGF-A and VEGF-R2.
Gheorghescu AK, Tywoniuk B, Duess J, Buchete NV, Thompson J
Toxicology and applied pharmacology289(1)79-88(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BFGF or VEGF gene therapy corrects insufficiency in the intrinsic healing capacity of tendons.
Title: Basic FGF or VEGF gene therapy corrects insufficiency in the intrinsic healing capacity of tendons.

VEGF expression in hyperoxia did not differ from normoxia on D16, yet, was actually approximately 75% higher in hyperoxia than in normoxia on day 18
Title: Chronic hypoxia and hyperoxia modifies morphology and VEGF concentration of the lungs of the developing chicken (Gallus gallus variant domesticus).

VEGF-A protein was decreased in cadmium treated extraembryonic membranes.
Title: Exposure of chick embryos to cadmium changes the extra-embryonic vascular branching pattern and alters expression of VEGF-A and VEGF-R2.

The results demonstrate that Vegf signaling could provide important insights into the complex relationship between bone and vasculature during intramembranous bone development.
Title: Vascular endothelial growth factor signaling affects both angiogenesis and osteogenesis during the development of scleral ossicles.

These results suggest that TRAIL inhibits VEGF-induced angiogenesis by increasing caspase-8 activity and subsequently decreasing non-apoptotic signaling functions of procaspase-8, without inducing caspase-3 activation and endothelial cell cytotoxicity.
Title: TRAIL negatively regulates VEGF-induced angiogenesis via caspase-8-mediated enzymatic and non-enzymatic functions.

The following VEGFA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VEGFA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00065	NM_205042.2 Latest version!	Gallus gallus vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OGa00187	NM_001110355.1 Latest version!	Gallus gallus vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VEGFA ( NM_205042.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990373.1 Gallus gallus vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OGa00065
Clone ID Related Accession (Same CDS sequence)	NM_205042.2
Accession Version	NM_205042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Gallus gallus(chicken)
Product	vascular endothelial growth factor A isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AM071319.1, AADN05000005.1 and AB011078.1. On Nov 16, 2007 this sequence version replaced NM_205042.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB011078.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGAACTTTC TGCTCACTTG GATCCACTGG GGGCTGGCGG CGCTGCTCTA TCTGCAGAGC 
GCGGAGTTGT CGAAGGCTGC TCCGGCCCTG GGGGATGGGG AGCGGAAGCC CAACGAAGTT 
ATCAAATTCC TGGAAGTCTA CGAACGCAGC TTCTGCAGGA CAATTGAGAC CCTGGTGGAC 
ATTTTCCAGG AGTACCCTGA TGAGGTGGAG TACATATTCA GGCCATCCTG TGTGCCTCTG 
ATGAGATGTG CGGGTTGCTG CGGCGATGAG GGCCTAGAAT GTGTCCCTGT GGATGTGTAC 
AACGTCACGA TGGAGATCGC AAGAATTAAA CCCCATCAGA GTCAGCACAT AGCGCACATG 
AGCTTCTTAC AGCACAGTAA ATGTGACTGC AGACCAAAGA AAGATGTCAA AAATAAACAA 
GAAAAAAAAT CAAAGCGAGG AAAGGGGAAG GGTCAAAAGA GAAAGCGCAA GAAAGGCCGG 
TACAAACCAC CCAGCTTTCA CTGTGAGCCT TGCTCAGAGA GGAGAAAGCA CTTGTTTGTA 
CAAGATCCCC AGACCTGTAA ATGTTCCTGC AAATTCACAG ACTCACGTTG CAAGTCGAGG 
CAGCTTGAGT TAAACGAGCG CACTTGCAGA TGTGAAAAAC CGAGACGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990373.1
CDS	530..1180
Translation	MNFLLTWIHWGLAALLYLQSAELSKAAPALGDGERKPNEVIKFLEVYERSFCRTIETLVDIFQEYPDEVEYIFRPSCVPLMRCAGCCGDEGLECVPVDVYNVTMEIARIKPHQSQHIAHMSFLQHSKCDCRPKKDVKNKQEKKSKRGKGKGQKRKRKKGRYKPPSFHCEPCSERRKHLFVQDPQTCKCSCKFTDSRCKSRQLELNERTCRCEKPRR

Target ORF information:

RefSeq Version	NM_205042.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus vascular endothelial growth factor A (VEGFA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205042.2

ORF Insert Sequence:

ATGAACTTTC TGCTCACTTG GATCCACTGG GGGCTGGCGG CGCTGCTCTA TCTGCAGAGC 
GCGGAGTTGT CGAAGGCTGC TCCGGCCCTG GGGGATGGGG AGCGGAAGCC CAACGAAGTT 
ATCAAATTCC TGGAAGTCTA CGAACGCAGC TTCTGCAGGA CAATTGAGAC CCTGGTGGAC 
ATTTTCCAGG AGTACCCTGA TGAGGTGGAG TACATATTCA GGCCATCCTG TGTGCCTCTG 
ATGAGATGTG CGGGTTGCTG CGGCGATGAG GGCCTAGAAT GTGTCCCTGT GGATGTGTAC 
AACGTCACGA TGGAGATCGC AAGAATTAAA CCCCATCAGA GTCAGCACAT AGCGCACATG 
AGCTTCTTAC AGCACAGTAA ATGTGACTGC AGACCAAAGA AAGATGTCAA AAATAAACAA 
GAAAAAAAAT CAAAGCGAGG AAAGGGGAAG GGTCAAAAGA GAAAGCGCAA GAAAGGCCGG 
TACAAACCAC CCAGCTTTCA CTGTGAGCCT TGCTCAGAGA GGAGAAAGCA CTTGTTTGTA 
CAAGATCCCC AGACCTGTAA ATGTTCCTGC AAATTCACAG ACTCACGTTG CAAGTCGAGG 
CAGCTTGAGT TAAACGAGCG CACTTGCAGA TGTGAAAAAC CGAGACGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VEGFA ( NM_001110355.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001103825.1 Gallus gallus vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OGa00187
Clone ID Related Accession (Same CDS sequence)	NM_001110355.1
Accession Version	NM_001110355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Gallus gallus(chicken)
Product	vascular endothelial growth factor A isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AM071319.1, AADN05000005.1, AY168004.1 and AB011078.1. Transcript Variant: This variant (2) is lacking an in-frame, coding exon compared to transcript variant 1, resulting in a shorter isoform (2) that is missing a 24 aa segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY168004.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGAACTTTC TGCTCACTTG GATCCACTGG GGGCTGGCGG CGCTGCTCTA TCTGCAGAGC 
GCGGAGTTGT CGAAGGCTGC TCCGGCCCTG GGGGATGGGG AGCGGAAGCC CAACGAAGTT 
ATCAAATTCC TGGAAGTCTA CGAACGCAGC TTCTGCAGGA CAATTGAGAC CCTGGTGGAC 
ATTTTCCAGG AGTACCCTGA TGAGGTGGAG TACATATTCA GGCCATCCTG TGTGCCTCTG 
ATGAGATGTG CGGGTTGCTG CGGCGATGAG GGCCTAGAAT GTGTCCCTGT GGATGTGTAC 
AACGTCACGA TGGAGATCGC AAGAATTAAA CCCCATCAGA GTCAGCACAT AGCGCACATG 
AGCTTCTTAC AGCACAGTAA ATGTGACTGC AGACCAAAGA AAGATGTCAA AAATAAACAA 
GAAAATCACT GTGAGCCTTG CTCAGAGAGG AGAAAGCACT TGTTTGTACA AGATCCCCAG 
ACCTGTAAAT GTTCCTGCAA ATTCACAGAC TCACGTTGCA AGTCGAGGCA GCTTGAGTTA 
AACGAGCGCA CTTGCAGATG TGAAAAACCG AGACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103825.1
CDS	530..1108
Translation	MNFLLTWIHWGLAALLYLQSAELSKAAPALGDGERKPNEVIKFLEVYERSFCRTIETLVDIFQEYPDEVEYIFRPSCVPLMRCAGCCGDEGLECVPVDVYNVTMEIARIKPHQSQHIAHMSFLQHSKCDCRPKKDVKNKQENHCEPCSERRKHLFVQDPQTCKCSCKFTDSRCKSRQLELNERTCRCEKPRR

Target ORF information:

RefSeq Version	NM_001110355.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus vascular endothelial growth factor A (VEGFA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110355.1

ORF Insert Sequence:

ATGAACTTTC TGCTCACTTG GATCCACTGG GGGCTGGCGG CGCTGCTCTA TCTGCAGAGC 
GCGGAGTTGT CGAAGGCTGC TCCGGCCCTG GGGGATGGGG AGCGGAAGCC CAACGAAGTT 
ATCAAATTCC TGGAAGTCTA CGAACGCAGC TTCTGCAGGA CAATTGAGAC CCTGGTGGAC 
ATTTTCCAGG AGTACCCTGA TGAGGTGGAG TACATATTCA GGCCATCCTG TGTGCCTCTG 
ATGAGATGTG CGGGTTGCTG CGGCGATGAG GGCCTAGAAT GTGTCCCTGT GGATGTGTAC 
AACGTCACGA TGGAGATCGC AAGAATTAAA CCCCATCAGA GTCAGCACAT AGCGCACATG 
AGCTTCTTAC AGCACAGTAA ATGTGACTGC AGACCAAAGA AAGATGTCAA AAATAAACAA 
GAAAATCACT GTGAGCCTTG CTCAGAGAGG AGAAAGCACT TGTTTGTACA AGATCCCCAG 
ACCTGTAAAT GTTCCTGCAA ATTCACAGAC TCACGTTGCA AGTCGAGGCA GCTTGAGTTA 
AACGAGCGCA CTTGCAGATG TGAAAAACCG AGACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Avian leukosis virus subgroup J induces VEGF expression via NF-?B/PI3K-dependent IL-6 production.
Oncotarget7(49)80275-80287(2016 Dec)
Gao Y,Zhang Y,Yao Y,Guan X,Liu Y,Qi X,Wang Y,Liu C,Zhang Y,Gao H,Nair V,Wang X,Gao Y

Basic FGF or VEGF gene therapy corrects insufficiency in the intrinsic healing capacity of tendons.
Scientific reports620643(2016 Feb)
Tang JB,Wu YF,Cao Y,Chen CH,Zhou YL,Avanessian B,Shimada M,Wang XT,Liu PY

Chronic hypoxia and hyperoxia modifies morphology and VEGF concentration of the lungs of the developing chicken (Gallus gallus variant domesticus).
Respiratory physiology & neurobiology21985-94(2015 Dec)
Lewallen MA,Burggren WW

Exposure of chick embryos to cadmium changes the extra-embryonic vascular branching pattern and alters expression of VEGF-A and VEGF-R2.
Toxicology and applied pharmacology289(1)79-88(2015 Nov)
Gheorghescu AK,Tywoniuk B,Duess J,Buchete NV,Thompson J