P4HB cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	P4HB
Entrez Gene ID	374091
Full Name	prolyl 4-hydroxylase subunit beta
Synonyms	PDI
General protein information	Preferred Names prolyl 4-hydroxylase subunit beta Names protein disulfide-isomerase R-cognin cellular thyroid hormone-binding protein procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase-associated 1) procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide (protein disulfide isomerase; thyroid hormone binding protein p55) prolyl 4-hydroxylase beta-subunit prolyl 4-hydroxylase, beta polypeptide retina cognin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001198710.2	NP_001185639.2	protein disulfide-isomerase precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	P4HB	XP_540488.2
Mus musculus (house mouse)	P4hb	NP_035162.1
Rattus norvegicus (Norway rat)	P4hb	NP_037130.1
Gallus gallus (chicken)	P4HB	NP_001185639.1
Danio rerio (zebrafish)	p4hb	NP_998529.3
Eremothecium gossypii	AGOS_AFR718W	NP_986266.2
Arabidopsis thaliana (thale cress)	PDIL1-4	NP_851234.1
Xenopus tropicalis (tropical clawed frog)	p4hb	NP_001034820.1
Homo sapiens (human)	P4HB	NP_000909.2
Pan troglodytes (chimpanzee)	P4HB	NP_001233358.1
Drosophila melanogaster (fruit fly)	Pdi	NP_524079.1
Saccharomyces cerevisiae (baker's yeast)	PDI1	NP_009887.1
Neurospora crassa	NCU09223	XP_959167.1
Oryza sativa (rice)	Os02g0100100	NP_001045579.1
Caenorhabditis elegans (roundworm)	pdi-2	NP_508778.1
Arabidopsis thaliana (thale cress)	PDIL1-3	NP_191056.2
	LOC714702	XP_002800695.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012407	XP_551775.3
Bos taurus (cattle)	P4HB	NP_776560.1
Kluyveromyces lactis	KLLA0C01111g	XP_452244.1
Schizosaccharomyces pombe (fission yeast)	SPAC1F5.02	NP_592871.1
Magnaporthe oryzae (rice blast fungus)	MGG_05753	XP_003710672.1

Gene Ontology
Bibliography

Related articles in PubMed

cDNA for R-cognin: homology with a multifunctional protein.
Krishna Rao AS, Hausman RE
Proceedings of the National Academy of Sciences of the United States of America90(7)2950-4(1993 Apr)

Molecular cloning of a multifunctional chicken protein acting as the prolyl 4-hydroxylase beta-subunit, protein disulphide-isomerase and a cellular thyroid-hormone-binding protein. Comparison of cDNA-deduced amino acid sequences with those in other species.
Parkkonen T, Kivirikko KI, Pihlajaniemi T
The Biochemical journal256(3)1005-11(1988 Dec)

Structure of the gene encoding the beta-subunit of chicken prolyl 4-hydroxylase.
Nakazawa M, Aida T, Everson WV, Gonda MA, Hughes SH, Kao WW
Gene71(2)451-60(1988 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following P4HB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P4HB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28307	NM_001198710.2 Latest version!	Gallus gallus prolyl 4-hydroxylase subunit beta (P4HB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P4HB ( NM_001198710.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001185639.2 Gallus gallus prolyl 4-hydroxylase subunit beta (P4HB), mRNA.

Price & Availability↑

CloneID	OGa28307
Clone ID Related Accession (Same CDS sequence)	NM_001198710.2
Accession Version	NM_001198710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Gallus gallus(chicken)
Product	protein disulfide-isomerase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR524423.1 and CR353567.1. On Dec 4, 2014 this sequence version replaced NM_001198710.1.

ATGGCCGTGG TGCGGCTGCG CGCGATCGTA GCGCTGCTCT GCCTCGTGCC GCCGCTCGGC 
CTCGCGGAGC CCCTGGAGGA GGAGGACGGC GTCCTGGTGC TGCGCGCCGC CAACTTCGAG 
CAGGCCCTGG CGGCGCACCG CCACCTGCTC GTGGAGTTCT ACGCGCCGTG GTGCGGGCAC 
TGCAAGGCGT TGGCCCCGGA GTACGCGAAG GCGGCGGCGC AGCTGAAGGC GGAGGGTTCC 
GAGATCCGCC TGGCCAAGGT GGACGCCACG GAGGAGGCGG AGCTGGCGCA GCAGTTCGGC 
GTGCGCGGGT ACCCCACCAT CAAGTTCTTC CGCAACGGGG ACAAGGCCGC GCCCCGCGAG 
TACACAGCTG GCAGGGAAGC AGATGATATT GTCAGCTGGC TGAAGAAGCG CACGGGACCG 
GCTGCCACTA CCCTGACAGA TGCTGCTGCA GCAGAGACGT TGGTGGATTC CAGTGAAGTG 
GTTGTGATTG GTTTCTTTAA GGATGTGACA TCAGATGCTG CGAAGGAGTT TTTGTTGGCA 
GCAGAATCCG TGGATGACAT TCCTTTTGGG ATTTCTTCCA GCGCTGACGT CTTCTCCAAG 
TACCAGCTTA GCCAAGATGG AGTGGTCCTT TTCAAGAAGT TTGATGAAGG ACGTAACAAC 
TTTGAAGGGG ATCTTACCAA AGATAACTTG CTGAACTTCA TCAAGTCTAA TCAGCTGCCT 
CTTGTCATAG AGTTTACTGA GCAGACTGCT CCTAAAATCT TTGGAGGAGA GATCAAGACT 
CACATTCTGC TGTTCTTACC AAAAAGTGTG TCTGATTATG AAGGGAAATT GGACAACTTC 
AAGACTGCAG CTGGAAACTT CAAAGGGAAG ATCCTGTTCA TCTTCATAGA CAGTGACCAC 
AGTGATAATC AAAGGATTCT GGAGTTTTTT GGTCTAAAGA AAGAAGAATG TCCAGCTGTA 
CGTTTGATAA CATTAGAAGA AGAAATGACC AAATACAAAC CTGAGTCAGA TGACCTCACA 
GCAGACAAGA TCAAAGAATT CTGTAATAAA TTCCTGGAGG GCAAGATCAA GCCTCACCTG 
ATGAGCCAGG ATCTCCCTGA AGACTGGGAC AAGCAGCCTG TCAAAGTTCT TGTTGGGAAG 
AATTTTGAGG AAGTTGCTTT TGATGAGAAT AAGAATGTCT TTGTGGAGTT CTATGCCCCC 
TGGTGTGGTC ACTGTAAGCA GCTGGCTCCG ATCTGGGACA AGCTGGGAGA GACCTACAGA 
GACCATGAGA ACATTGTCAT TGCCAAGATG GATTCAACAG CCAATGAAGT AGAGGCAGTG 
AAAATTCACA GCTTCCCCAC GCTCAAGTTC TTCCCTGCAG GCTCTGGCAG AAACGTAATT 
GACTATAATG GAGAGAGGAC ACTAGAAGGC TTCAAAAAAT TCTTGGAGAG TGGAGGCCAG 
GATGGAGCAG CAGCTGATGA TGATCTGGAA GATCTGGAGA CAGATGAAGA AACGGACCTT 
GAGGAAGGTG ATGATGATGA GCAAAAAATC CAAAAGGATG AATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185639.2
CDS	58..1605
Translation	MAVVRLRAIVALLCLVPPLGLAEPLEEEDGVLVLRAANFEQALAAHRHLLVEFYAPWCGHCKALAPEYAKAAAQLKAEGSEIRLAKVDATEEAELAQQFGVRGYPTIKFFRNGDKAAPREYTAGREADDIVSWLKKRTGPAATTLTDAAAAETLVDSSEVVVIGFFKDVTSDAAKEFLLAAESVDDIPFGISSSADVFSKYQLSQDGVVLFKKFDEGRNNFEGDLTKDNLLNFIKSNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYEGKLDNFKTAAGNFKGKILFIFIDSDHSDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDDLTADKIKEFCNKFLEGKIKPHLMSQDLPEDWDKQPVKVLVGKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYRDHENIVIAKMDSTANEVEAVKIHSFPTLKFFPAGSGRNVIDYNGERTLEGFKKFLESGGQDGAAADDDLEDLETDEETDLEEGDDDEQKIQKDEL

Target ORF information:

RefSeq Version	NM_001198710.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus prolyl 4-hydroxylase subunit beta (P4HB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198710.2

ORF Insert Sequence:

ATGGCCGTGG TGCGGCTGCG CGCGATCGTA GCGCTGCTCT GCCTCGTGCC GCCGCTCGGC 
CTCGCGGAGC CCCTGGAGGA GGAGGACGGC GTCCTGGTGC TGCGCGCCGC CAACTTCGAG 
CAGGCCCTGG CGGCGCACCG CCACCTGCTC GTGGAGTTCT ACGCGCCGTG GTGCGGGCAC 
TGCAAGGCGT TGGCCCCGGA GTACGCGAAG GCGGCGGCGC AGCTGAAGGC GGAGGGTTCC 
GAGATCCGCC TGGCCAAGGT GGACGCCACG GAGGAGGCGG AGCTGGCGCA GCAGTTCGGC 
GTGCGCGGGT ACCCCACCAT CAAGTTCTTC CGCAACGGGG ACAAGGCCGC GCCCCGCGAG 
TACACAGCTG GCAGGGAAGC AGATGATATT GTCAGCTGGC TGAAGAAGCG CACGGGACCG 
GCTGCCACTA CCCTGACAGA TGCTGCTGCA GCAGAGACGT TGGTGGATTC CAGTGAAGTG 
GTTGTGATTG GTTTCTTTAA GGATGTGACA TCAGATGCTG CGAAGGAGTT TTTGTTGGCA 
GCAGAATCCG TGGATGACAT TCCTTTTGGG ATTTCTTCCA GCGCTGACGT CTTCTCCAAG 
TACCAGCTTA GCCAAGATGG AGTGGTCCTT TTCAAGAAGT TTGATGAAGG ACGTAACAAC 
TTTGAAGGGG ATCTTACCAA AGATAACTTG CTGAACTTCA TCAAGTCTAA TCAGCTGCCT 
CTTGTCATAG AGTTTACTGA GCAGACTGCT CCTAAAATCT TTGGAGGAGA GATCAAGACT 
CACATTCTGC TGTTCTTACC AAAAAGTGTG TCTGATTATG AAGGGAAATT GGACAACTTC 
AAGACTGCAG CTGGAAACTT CAAAGGGAAG ATCCTGTTCA TCTTCATAGA CAGTGACCAC 
AGTGATAATC AAAGGATTCT GGAGTTTTTT GGTCTAAAGA AAGAAGAATG TCCAGCTGTA 
CGTTTGATAA CATTAGAAGA AGAAATGACC AAATACAAAC CTGAGTCAGA TGACCTCACA 
GCAGACAAGA TCAAAGAATT CTGTAATAAA TTCCTGGAGG GCAAGATCAA GCCTCACCTG 
ATGAGCCAGG ATCTCCCTGA AGACTGGGAC AAGCAGCCTG TCAAAGTTCT TGTTGGGAAG 
AATTTTGAGG AAGTTGCTTT TGATGAGAAT AAGAATGTCT TTGTGGAGTT CTATGCCCCC 
TGGTGTGGTC ACTGTAAGCA GCTGGCTCCG ATCTGGGACA AGCTGGGAGA GACCTACAGA 
GACCATGAGA ACATTGTCAT TGCCAAGATG GATTCAACAG CCAATGAAGT AGAGGCAGTG 
AAAATTCACA GCTTCCCCAC GCTCAAGTTC TTCCCTGCAG GCTCTGGCAG AAACGTAATT 
GACTATAATG GAGAGAGGAC ACTAGAAGGC TTCAAAAAAT TCTTGGAGAG TGGAGGCCAG 
GATGGAGCAG CAGCTGATGA TGATCTGGAA GATCTGGAGA CAGATGAAGA AACGGACCTT 
GAGGAAGGTG ATGATGATGA GCAAAAAATC CAAAAGGATG AATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

cDNA for R-cognin: homology with a multifunctional protein.
Proceedings of the National Academy of Sciences of the United States of America90(7)2950-4(1993 Apr)
Krishna Rao AS,Hausman RE

Molecular cloning of a multifunctional chicken protein acting as the prolyl 4-hydroxylase beta-subunit, protein disulphide-isomerase and a cellular thyroid-hormone-binding protein. Comparison of cDNA-deduced amino acid sequences with those in other species.
The Biochemical journal256(3)1005-11(1988 Dec)
Parkkonen T,Kivirikko KI,Pihlajaniemi T

Structure of the gene encoding the beta-subunit of chicken prolyl 4-hydroxylase.
Gene71(2)451-60(1988 Nov)
Nakazawa M,Aida T,Everson WV,Gonda MA,Hughes SH,Kao WW