HCRT cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	HCRT
Entrez Gene ID	374005
Full Name	hypocretin neuropeptide precursor
General protein information	Preferred Names hypocretin neuropeptide precursor Names orexin hypocretin (orexin) neuropeptide
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204185.2	NP_989516.1	orexin precursor
XM_015299567.1	XP_015155053.1	orexin isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Orexin in the chicken hypothalamus: immunocytochemical localisation and comparison of mRNA concentrations during the day and night, and after chronic food restriction.
Miranda B, Esposito V, de Girolamo P, Sharp PJ, Wilson PW, Dunn IC
Brain research151334-40(2013 Jun)

Molecular cloning of chicken prepro-orexin cDNA and preferential expression in the chicken hypothalamus.
Ohkubo T, Boswell T, Lumineau S
Biochimica et biophysica acta1577(3)476-80(2002 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study found evidence that hypothalamic orexin plays a role in the sleep-wake cycle and it may be involved in aspects of energy balance.
Title: Orexin in the chicken hypothalamus: immunocytochemical localisation and comparison of mRNA concentrations during the day and night, and after chronic food restriction.

The following HCRT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCRT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00946	NM_204185.2 Latest version!	Gallus gallus hypocretin neuropeptide precursor (HCRT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	Quote Price
OGa46959	XM_015299567.1 Latest version!	Gallus gallus hypocretin (orexin) neuropeptide precursor (HCRT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HCRT ( NM_204185.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989516.1 Gallus gallus hypocretin neuropeptide precursor (HCRT), mRNA.

Price & Availability↑

CloneID	OGa00946
Clone ID Related Accession (Same CDS sequence)	NM_204185.2
Accession Version	NM_204185.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	orexin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB056748.1. On Aug 30, 2012 this sequence version replaced NM_204185.1. ##Evidence-Data-START## Transcript exon combination :: AB056748.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAGGTGC CCAACGCCAA GCTGCAGCGG TCCGCCTGCC TTCTGCTGCT GCTGCTGCTG 
CTCTGCTCCC TGGCCGGGGG GCGGCAGAGC CTGCCCGAGT GCTGCCGGCA GAAAACCTGC 
TCCTGCCGTA TCTACGACCT CCTGCACGGC ATGGGCAACC ACGCCGCCGG CATCCTCACG 
CTGGGCAAGA GGAAGAGCAT CCCGCCAGCC TTCCAGAGCC GGCTGTACCG CCTGCTGCAC 
GGCTCCGGCA ACCACGCAGC GGGCATCCTC ACCATAGGCA AACGTGAGGA GCGCCCCGGC 
ACCGCCTGCC GCGACGCGCT GAGCTGTGCT GCGGGCACTC AGCCTACGGT GACCCCGAGG 
GGCACTGCTG CCAGCCCCAG GGAGTGCCAG GAGCACGCTG AGAAGGACCT GACCAAGGGC 
TGGGCAGCGG CAAAGAGCTT TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989516.1
CDS	49..495
Translation	MEVPNAKLQRSACLLLLLLLLCSLAGGRQSLPECCRQKTCSCRIYDLLHGMGNHAAGILTLGKRKSIPPAFQSRLYRLLHGSGNHAAGILTIGKREERPGTACRDALSCAAGTQPTVTPRGTAASPRECQEHAEKDLTKGWAAAKSFY

Target ORF information:

RefSeq Version	NM_204185.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus hypocretin neuropeptide precursor (HCRT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204185.2

ORF Insert Sequence:

ATGGAGGTGC CCAACGCCAA GCTGCAGCGG TCCGCCTGCC TTCTGCTGCT GCTGCTGCTG 
CTCTGCTCCC TGGCCGGGGG GCGGCAGAGC CTGCCCGAGT GCTGCCGGCA GAAAACCTGC 
TCCTGCCGTA TCTACGACCT CCTGCACGGC ATGGGCAACC ACGCCGCCGG CATCCTCACG 
CTGGGCAAGA GGAAGAGCAT CCCGCCAGCC TTCCAGAGCC GGCTGTACCG CCTGCTGCAC 
GGCTCCGGCA ACCACGCAGC GGGCATCCTC ACCATAGGCA AACGTGAGGA GCGCCCCGGC 
ACCGCCTGCC GCGACGCGCT GAGCTGTGCT GCGGGCACTC AGCCTACGGT GACCCCGAGG 
GGCACTGCTG CCAGCCCCAG GGAGTGCCAG GAGCACGCTG AGAAGGACCT GACCAAGGGC 
TGGGCAGCGG CAAAGAGCTT TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCRT ( XM_015299567.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015155053.1 Gallus gallus hypocretin (orexin) neuropeptide precursor (HCRT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa46959
Clone ID Related Accession (Same CDS sequence)	XM_015299567.1
Accession Version	XM_015299567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	orexin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456049.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGC CCAACGCCAA GCGGTCCGCC TGCCTTCTGC TGCTGCTGCT GCTGCTCTGC 
TCCCTGGCCG GGGGGCGGCA GAGCCTGCCC GAGTGCTGCC GGCAGAAAAC CTGCTCCTGC 
CGTATCTACG ACCTCCTGCA CGGCATGGGC AACCACGCCG CCGGCATCCT CACGCTGGGC 
AAGAGGAAGA GCATCCCGCC AGCCTTCCAG AGCCGGCTGT ACCGCCTGCT GCACGGCTCC 
GGCAACCACG CAGCGGGCAT CCTCACCATG GGCAAACGTG AGGAGCGCCC CGGCACCGCC 
TGCCGCGACG CGCTGAGCTG TGCTGCGGGC ACTCAGCCTA CGGTGACCCC GAGGGGCACT 
GCTGCCAGCC CCAGGGAGTG CCAGGAGCAC GCTGAGAAGG ACCTGACCAA GGGCTGGGCA 
GCGGCAAAGA GCTTTTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015155053.1
CDS	468..908
Translation	MEVPNAKRSACLLLLLLLLCSLAGGRQSLPECCRQKTCSCRIYDLLHGMGNHAAGILTLGKRKSIPPAFQSRLYRLLHGSGNHAAGILTMGKREERPGTACRDALSCAAGTQPTVTPRGTAASPRECQEHAEKDLTKGWAAAKSFY

Target ORF information:

RefSeq Version	XM_015299567.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus hypocretin (orexin) neuropeptide precursor (HCRT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015299567.1

ORF Insert Sequence:

ATGGAGGTGC CCAACGCCAA GCGGTCCGCC TGCCTTCTGC TGCTGCTGCT GCTGCTCTGC 
TCCCTGGCCG GGGGGCGGCA GAGCCTGCCC GAGTGCTGCC GGCAGAAAAC CTGCTCCTGC 
CGTATCTACG ACCTCCTGCA CGGCATGGGC AACCACGCCG CCGGCATCCT CACGCTGGGC 
AAGAGGAAGA GCATCCCGCC AGCCTTCCAG AGCCGGCTGT ACCGCCTGCT GCACGGCTCC 
GGCAACCACG CAGCGGGCAT CCTCACCATG GGCAAACGTG AGGAGCGCCC CGGCACCGCC 
TGCCGCGACG CGCTGAGCTG TGCTGCGGGC ACTCAGCCTA CGGTGACCCC GAGGGGCACT 
GCTGCCAGCC CCAGGGAGTG CCAGGAGCAC GCTGAGAAGG ACCTGACCAA GGGCTGGGCA 
GCGGCAAAGA GCTTTTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Orexin in the chicken hypothalamus: immunocytochemical localisation and comparison of mRNA concentrations during the day and night, and after chronic food restriction.
Brain research151334-40(2013 Jun)
Miranda B,Esposito V,de Girolamo P,Sharp PJ,Wilson PW,Dunn IC

Molecular cloning of chicken prepro-orexin cDNA and preferential expression in the chicken hypothalamus.
Biochimica et biophysica acta1577(3)476-80(2002 Sep)
Ohkubo T,Boswell T,Lumineau S