LOC110367855 cDNA ORF clone, Fundulus heteroclitus(mummichog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110367855
Entrez Gene ID	110367855
Full Name	E3 ubiquitin/ISG15 ligase TRIM25-like
Gene Type	protein-coding
Organism	Fundulus heteroclitus(mummichog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021314528.1	XP_021170203.1	E3 ubiquitin/ISG15 ligase TRIM25-like

Pathways from BioSystems
Gene Ontology

The following LOC110367855 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110367855 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFu135099	XM_021314528.1 Latest version!	Fundulus heteroclitus E3 ubiquitin/ISG15 ligase TRIM25-like (LOC110367855), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110367855 ( XM_021314528.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021170203.1 Fundulus heteroclitus E3 ubiquitin/ISG15 ligase TRIM25-like (LOC110367855), mRNA.

Price & Availability↑

CloneID	OFu135099
Clone ID Related Accession (Same CDS sequence)	XM_021314528.1
Accession Version	XM_021314528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	E3 ubiquitin/ISG15 ligase TRIM25-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225972.1) and transcript sequence (GCES01151161.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 942 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGATCAGA AAGGCGATGA GCTGGACCGA GAAACTTTTT CCTGTGCGAT CTGTCTGGAT 
CTACTGAAAG ATCCTGTGAC TATTCCGTGT GGACACAGCT ACTGTATGAA GTGTATTAAA 
AACTTCTGGG ATGAAGAGGA TCACAAAGGA ATCCACAGCT GCCCTCAGTG CAGGAAAACT 
TTTCTACCGA GGCCTGAGCT GAGTAAAAAC ACCATGTTAG CAGCTTTAGT GGAGCAGCTG 
AAGAAGACTG GACTCCAAGC TGCTCCTGCT GATCTCTGCT ATGCTGGACC TGAAGATGTG 
GCCTGTGATT TCTGCTCTGG AAGAAAACGA AAAGCCACCA AGTCCTGTTT AGTCTGTCTG 
ACCTCTTTCT GTGAGAAACA CCTTCAGCCT CATTATGATG TGGCTCCACT AAAGAAACAC 
AAGCTGGTGG AGCCCTCCAA GAACCTCCAG GAGAACATCT GCTCTCGTCA TGATGAGGTG 
ATGAAGATGT TCTGTCGTAC TGATCAGAAG TGTATCTGTT ATCTCTGCTC TGTGGATGAA 
TATAAAGGCC ACGACACAGT GTCAGCTGCA GCAGAAAGGA CTGAGAGGCA GAGAGAGCTG 
GAGGTGAGAC GAGAAAACAT CCAGCAGAGA ATCCAGGACA GAGAGAAAGA TGTGAAGCTG 
CTTCAACAGG AGCTGGAGGC CATCAAGCAC TCTGCTGATA AAACAGTGGA GGACAGTGAG 
AAGATCTTCA CTCAGCTGAT CCGTCTCATC CAGAAAAGAA GCTCTGATGT GAAGCAGCAG 
ATCAGATCCC AGCAGGAAAC TGAAGGGAGT CGAGTCAAAG AGCTTGAGGA GAAGCTGGAG 
CAGGAGATCA CTGAGCTGAA GAGGAAAGAC GCTGAGCTGA AGCAGCTCTC AGACACAGAG 
GATCACAACC AGTTTCTCCA CAACTACCCC TCACTGTCAG CACTCAGTGA GTCTACACAC 
TCATCCAGCA TCAAGATCCG TCCTCTGAGC TACTTTGAGG ATGTGACAGC AGCTGTGTCA 
GAGCTCAGAG ATCAACTACA GGACATCCTG AGAGACGCAT GGACAAACAT CTCACTGAGA 
CTCACTGAGG TGGATGTTTT ACTGTCAGAA CCAGAACCAG AGAACAGAGC TGGATTCTTG 
AGATATTCAT GTGAAATCAC ACTGGATCCA AACACAGCAA ACAGTTTTCT GTTACTATCA 
GAAGGGAACA GGAAGGTGAC ATTGATCAAT CAACCTCAGT CTTATTCTAG TCATCCAGAC 
AGATTCACTG GTTGTTGTCA GGTTCTGAGT AGAGAGAGTC TGACTGGACG TTGTTATTGG 
GAGGTGGAGA GGAGAGAAGA AGTTTATGTA GCAGTCGCAT ACAAGAATAT CAGCAGAGCA 
GGAGGTTGGA ATGAATGTGG ATTTGGATAT AATGACAAAT CTTGGGCAAT ATATTGTTAC 
CAAGGAGGTT ATAAATTTGG TCACAACAGC ATCTGGACCT CCATATCAGG TCCTGGTTCC 
TCCAGAGTAG GAGTGTACCT GGATCACAGA GCAGGTATTC TGTCCTTCTA CAGCGTCTCT 
GAAACCATGA CTCTCCTCCA CAGAGTCCAG ACCACCTTCA CTCAGCCGCT ACATGCTGGA 
GTTTGGGTTT ACATCTATGG AGGATCTGCT GAGTTCTGTA AACCCAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021170203.1
CDS	106..1776
Translation	MDQKGDELDRETFSCAICLDLLKDPVTIPCGHSYCMKCIKNFWDEEDHKGIHSCPQCRKTFLPRPELSKNTMLAALVEQLKKTGLQAAPADLCYAGPEDVACDFCSGRKRKATKSCLVCLTSFCEKHLQPHYDVAPLKKHKLVEPSKNLQENICSRHDEVMKMFCRTDQKCICYLCSVDEYKGHDTVSAAAERTERQRELEVRRENIQQRIQDREKDVKLLQQELEAIKHSADKTVEDSEKIFTQLIRLIQKRSSDVKQQIRSQQETEGSRVKELEEKLEQEITELKRKDAELKQLSDTEDHNQFLHNYPSLSALSESTHSSSIKIRPLSYFEDVTAAVSELRDQLQDILRDAWTNISLRLTEVDVLLSEPEPENRAGFLRYSCEITLDPNTANSFLLLSEGNRKVTLINQPQSYSSHPDRFTGCCQVLSRESLTGRCYWEVERREEVYVAVAYKNISRAGGWNECGFGYNDKSWAIYCYQGGYKFGHNSIWTSISGPGSSRVGVYLDHRAGILSFYSVSETMTLLHRVQTTFTQPLHAGVWVYIYGGSAEFCKPK

Target ORF information:

RefSeq Version	XM_021314528.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus E3 ubiquitin/ISG15 ligase TRIM25-like (LOC110367855), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021314528.1

ORF Insert Sequence:

ATGGATCAGA AAGGCGATGA GCTGGACCGA GAAACTTTTT CCTGTGCGAT CTGTCTGGAT 
CTACTGAAAG ATCCTGTGAC TATTCCGTGT GGACACAGCT ACTGTATGAA GTGTATTAAA 
AACTTCTGGG ATGAAGAGGA TCACAAAGGA ATCCACAGCT GCCCTCAGTG CAGGAAAACT 
TTTCTACCGA GGCCTGAGCT GAGTAAAAAC ACCATGTTAG CAGCTTTAGT GGAGCAGCTG 
AAGAAGACTG GACTCCAAGC TGCTCCTGCT GATCTCTGCT ATGCTGGACC TGAAGATGTG 
GCCTGTGATT TCTGCTCTGG AAGAAAACGA AAAGCCACCA AGTCCTGTTT AGTCTGTCTG 
ACCTCTTTCT GTGAGAAACA CCTTCAGCCT CATTATGATG TGGCTCCACT AAAGAAACAC 
AAGCTGGTGG AGCCCTCCAA GAACCTCCAG GAGAACATCT GCTCTCGTCA TGATGAGGTG 
ATGAAGATGT TCTGTCGTAC TGATCAGAAG TGTATCTGTT ATCTCTGCTC TGTGGATGAA 
TATAAAGGCC ACGACACAGT GTCAGCTGCA GCAGAAAGGA CTGAGAGGCA GAGAGAGCTG 
GAGGTGAGAC GAGAAAACAT CCAGCAGAGA ATCCAGGACA GAGAGAAAGA TGTGAAGCTG 
CTTCAACAGG AGCTGGAGGC CATCAAGCAC TCTGCTGATA AAACAGTGGA GGACAGTGAG 
AAGATCTTCA CTCAGCTGAT CCGTCTCATC CAGAAAAGAA GCTCTGATGT GAAGCAGCAG 
ATCAGATCCC AGCAGGAAAC TGAAGGGAGT CGAGTCAAAG AGCTTGAGGA GAAGCTGGAG 
CAGGAGATCA CTGAGCTGAA GAGGAAAGAC GCTGAGCTGA AGCAGCTCTC AGACACAGAG 
GATCACAACC AGTTTCTCCA CAACTACCCC TCACTGTCAG CACTCAGTGA GTCTACACAC 
TCATCCAGCA TCAAGATCCG TCCTCTGAGC TACTTTGAGG ATGTGACAGC AGCTGTGTCA 
GAGCTCAGAG ATCAACTACA GGACATCCTG AGAGACGCAT GGACAAACAT CTCACTGAGA 
CTCACTGAGG TGGATGTTTT ACTGTCAGAA CCAGAACCAG AGAACAGAGC TGGATTCTTG 
AGATATTCAT GTGAAATCAC ACTGGATCCA AACACAGCAA ACAGTTTTCT GTTACTATCA 
GAAGGGAACA GGAAGGTGAC ATTGATCAAT CAACCTCAGT CTTATTCTAG TCATCCAGAC 
AGATTCACTG GTTGTTGTCA GGTTCTGAGT AGAGAGAGTC TGACTGGACG TTGTTATTGG 
GAGGTGGAGA GGAGAGAAGA AGTTTATGTA GCAGTCGCAT ACAAGAATAT CAGCAGAGCA 
GGAGGTTGGA ATGAATGTGG ATTTGGATAT AATGACAAAT CTTGGGCAAT ATATTGTTAC 
CAAGGAGGTT ATAAATTTGG TCACAACAGC ATCTGGACCT CCATATCAGG TCCTGGTTCC 
TCCAGAGTAG GAGTGTACCT GGATCACAGA GCAGGTATTC TGTCCTTCTA CAGCGTCTCT 
GAAACCATGA CTCTCCTCCA CAGAGTCCAG ACCACCTTCA CTCAGCCGCT ACATGCTGGA 
GTTTGGGTTT ACATCTATGG AGGATCTGCT GAGTTCTGTA AACCCAAATA G