LOC105938657 cDNA ORF clone, Fundulus heteroclitus(mummichog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105938657
Entrez Gene ID	105938657
Full Name	slit homolog 1 protein
Synonyms	slit1
Gene Type	protein-coding
Organism	Fundulus heteroclitus(mummichog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012880473.1	XP_012735927.1	slit homolog 1 protein isoform X3
XM_012880474.1	XP_012735928.1	slit homolog 1 protein isoform X4
XM_012880473.2	XP_012735927.1	slit homolog 1 protein isoform X2
XM_012880471.1	XP_012735925.1	slit homolog 1 protein isoform X1
XM_012880472.1	XP_012735926.1	slit homolog 1 protein isoform X2
XM_012880471.2	XP_012735925.1	slit homolog 1 protein isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC105938657 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105938657 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFu124012	XM_012880471.2 Latest version!	Fundulus heteroclitus slit homolog 1 protein (LOC105938657), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OFu124013	XM_012880472.1 Latest version!	Fundulus heteroclitus slit homolog 1 (Drosophila) (slit1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OFu124014	XM_012880473.2 Latest version!	Fundulus heteroclitus slit homolog 1 protein (LOC105938657), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OFu124015	XM_012880474.1 Latest version!	Fundulus heteroclitus slit homolog 1 (Drosophila) (slit1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OFu124014	XM_012880473.1	Fundulus heteroclitus slit homolog 1 (Drosophila) (slit1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OFu124012	XM_012880471.1	Fundulus heteroclitus slit homolog 1 (Drosophila) (slit1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105938657 ( XM_012880471.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012735925.1 Fundulus heteroclitus slit homolog 1 (Drosophila) (slit1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFu124012
Clone ID Related Accession (Same CDS sequence)	XM_012880471.1 , XM_012880471.2
Accession Version	XM_012880471.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	slit homolog 1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234518.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA GAGCAAACAC CAACTCCACA AAGACGAGAC AAGAGAGGAG TTTTCAAGAA 
GGAAAGAAAG AAAGAAAGAA AGAAACCATC CCTTTTTTTT TTACATGGAC AAATTATAGT 
GGATATCAAA GCTGCATCAT CAGATCTGTA GGATTTGTGT GGAAGATCTT TTTTTTTTTT 
CTCCCTTCTA TTTATACTCT CCAGTTAAAT AAAATTGTTC CTGCAGGACT TGTGAGGTGT 
GGCAGGACTT TTTTGGAAGC CAAAAGGAAG GAGAACATAA AGAGAGGTGG ACAGAAACAA 
GTAAGGAGGG GAAAAAAAAA GAAGAAAAGG CTTAGGAACA TGCCCGCTGA AGACAGCAGT 
TTTGAATCAA GCATGGGCAG GCTGGCTGGG CAGTGGATGT GGCTGCTGAT GCTCTCTGCC 
TTGATGCTGG GCGATGCCAG CGCCTGCCCG GCGCTTTGTA CCTGCAGCGG CACCACAGTG 
GACTGCCACG GACTGGGGCT CAAAACTATG CCAAGGAGTA TCCCCCGCAA CACTGAGAGA 
CTGGAGCTGA ACGGAAACAA CCTCACACGC ATCACCAAGA GTGACTTTGC TGGCCTGAAG 
TACCTCAGAG TGCTACAGCT GATGGAGAAT CAGATTACAG TGATTGAGCG AGGAGCTTTC 
GATGACATGA AGGAGCTGGA GCGGCTGCGG CTGAACCGTA ATCAACTGCA GCAGCTTCCG 
GAGCTGCTGT TTCAGAAGAA CCCCGGCCTG TCTCGGCTCG ATCTGAGTGA GAACTTCATC 
CAATCCATCC CCAGAAAAGC CTTCAGAGGA GCGACAGACA TCAAAAACCT GCAGCTGGAT 
AAAAACCACA TCGGCTGCAT CGAGGATGGA GCCTTCCGAG CTCTAAGAGG CCTGGAAGTA 
CTAACGCTGA ACAACAACAA CATCAGCAGC ATCCCCGTCT CCAGCTTCAA CCACATGCCC 
AAACTCCGAA CTTTCCGTCT TCACTCCAAC AACTTAAACT GTGATTGTCA CCTGGCCTGG 
CTGTCCCAGT GGCTCAGACA GAGGCCTACG ATTGGTCTGT TCACCCAGTG TGCTTCGCCG 
CCAGAGCTAA GAGGACTCAA TGTGGCGGAG GTCCAGAAAC ACGAATTCTC CTGTTCAGGG 
CATCAGGAGT CTTCCAGCCT GCAGCCGTGC AGCATTGGCG GAGGCTCATG TCCCGCCATG 
TGCACCTGCA GTAACAACAT CGTGGACTGC AGAGGGAAAG GTCTCACTGC CATTCCAGCC 
AATCTGCCGG ACAGCATGTC AGAAATACGT CTGGAGCAGA ACGGGATTAA GTCTGTGCCT 
CCCGGAGCCT TCTCCCCCTA CAAGAAACTG CGCAGGATAG ACCTGAGCAA CAACCAGATC 
TCAGAGATAG CGCCTGACGC CTTCCAGGGA CTGCGATCTC TTAACTCTCT CGTGCTTTAC 
GGAAACAAGA TCACCGACCT GCCGAAGGGT GTGTTTGACG GCCTCTACTC TTTGCAACTG 
CTGCTGCTGA ATGCCAATAA GATTCACTGC GTCCGTGCCA ATGCCTTCCA GGATCTGCAG 
AATCTCTCCC TGCTATCGCT GTACGATAAC AAGATCCAAA CCCTCGCCAA GGGCACCTTC 
ACTTCCCTGC GAGCAATACA AACCCTGCAC CTGGCGCAGA ACCCGTTTAT TTGTGACTGT 
AATCTGAAGT GGCTGGCAGA CTACCTGCGC TCCAACCCAA TCGAGACCAG CGGCGCCCGC 
TGCGCCAGCC CACGGCGCCT CGCAAACAAA CGCATCGGAC AAATCAAGAG CAAGAAGTTC 
CGCTGCTCCG CCAAAGAGCA GTACGTCATC CCAGGTACAG AAGACACCCG TCTCAATAAC 
GCCTGCAACA CTGATCCCGT GTGTCCTCCC AAGTGTCGCT GTGAGTCTAA CGTAGTGGAC 
TGCTCCAACC TGAAACTTAC CAAGATCCCC GAGCACATCC CTGCATCCAC AGCTGAACTC 
CGCCTTAACA ATAACGAGAT CACCAGCCTT GAGGCAACTG GAGTCTTTAA GAACCTTTCC 
CATCTGAAGA AAATTAACCT GAGTAACAAC AAGATCACAG AGATCGAGGA CGGGGCTTTT 
GAAGGTGCAT CCTCTGTCAT TGAGCTCCAC CTCACAGCCA ATCAAATCGA CTCGGTTCGC 
AGCGGGATGT TCCGAGGCCT CGAGGGACTG CGCATGATGA TGCTGCGGAA CAACAAAATC 
AGCTGTGTCC ACAACGACAG CTTCACGGGG CTTCACAACG TCCGTCTGCT GTCTCTGTAC 
GACAACCACC TGACCACCAT TACTCCTGGA GCATTCGACA CGCTGCAGAC TCTCTCTACT 
CTAAACCTCT TGGCCAACTC CTTCAACTGC GACTGCCGGC TGGCGTGGCT CGGCGACTGG 
CTGAGGAGCA GGAAGATTGT GACGGGTAAC CCTCGCTGCC AGCGCCCGGC CTTCCTGAAG 
GAGATCCCCC TGCAGGATGT AGCGCTGCCG GATTTTCGCT GCGAGGAAGG CCAAGAGGAG 
TCAAGCTGTG TTCCTCGACC CCAGTGTCCC AGCGAGTGTA CTTGCCTAGA GACGGTGGTG 
CGCTGCAGCA ACAAGCATCT CCACACACTA CCCAAAGGCA TCCCCAGGAA TGTGACTGAA 
CTGTACCTTG ATGGCAACCA GTTCAGTGTT GTTCCCAAGG AACTGTCTTC CTTCAAATTC 
CTACAGCTGG TTGACCTGAG CAACAACAAA ATCAACTCTC TGACCAATTC ATCCTTTGCT 
AACATGAGCC AACTGACCAC TCTAATCCTG AGCTACAACT CCCTGCGCTG CATACCCAAA 
ATGGCTTTCA GTGGCCTGCA CTCACTGCGA CTGCTGTCAC TTCATGGTAA TGAAATCTCA 
GAGCTTCCAG ATGGCATCTT CAATGATGTG ACCTCCCTCT CCCATTTGGC GATCGGAGCC 
AACCCTCTGC ACTGTGACTG CCACCTGCGC TGGCTCTCCG ACTGGGTGAA GACCGGATAC 
AAAGAACCTG GGATCGCGCG CTGTGCCGGC CCACAGGGGA TGGAGAGCAA GCTGCTCCTC 
ACCACGCCTG CCAAGAAATT TGAATGCACA GGCGATGTGG ACACGTCGGT GCTGGCCAAG 
TGTAACCCCT GCCTGTCCAG TCCCTGCCTG AATCACGGCA TCTGTCACAA TGACCCATTG 
GAGATCTACA GGTGCAGCTG TCCTCCTGGG TTCAAGGGCA AGAACTGTGA GACGGCTGCG 
AATGCCTGTG TGAGTAGCCC ATGCGCCAAT GGAGGAACCT GCAAAGTGAA TGAGGAGGAC 
GAGGGAGAGT ACCGCTGCTC CTGTCTGCCG GGCTTCGAGG GCCCGACTTG CCAAATCAAT 
GTTGACGACT GTGAAGACAA CGACTGTGAG AACGGGGCGA CCTGCATTGA TGGAGTCAAC 
AACTACACTT GTTTTTGTCC CCCCTACTAC ACAGGGGAGA TGTGTGAAGA AATGGACGAT 
GTGTGTGCTC CAGGGCGAAG CCCCTGCCAA CATCAGTCTA CCTGCCTCAT CAGCACCTCT 
GGACCCAAGT GTGTGTGTTC TCCTGGGTAT GTTGGTGATG ACTGCAGTGT CAACTACGAT 
GACTGCAGGG AGCATCGCTG TCAGAACGGA GCACAGTGCG TGGATGAGCT GAACGGATAT 
TCCTGCATCT GCCCCGAAGG ATATAGCGGC CAGCTGTGTG AAGTTCCCCC ATCTCCCCTG 
TCACTGTGTG AGCTGGCCGA CTGCCAAAAC AACGCCCCCT GTGTGGAGCG AGGTGGCCGC 
GCCCTCTGCC AGTGTCCTCC AGAGTTCGGG GGTCCGCGCT GCGAAAAACT GGTCAGCGTC 
AACTTTGTCG ACAGAGACTC CTACTTATTG CTGTCCGACC TGGAGAACTG GCCACAAGCT 
AACATCACCC TGCAGGTTTC CACAGCTGAG GACAACGGGA TCCTGCTGTA CAATGGAGAC 
AACGATCACA TTGCAGTGGA GCTCTTCCAA GGCCACGTGA AAGTGAGCTA CGACCCGGGC 
AGCCAGCCAG GACACGCCAT ATACAGCACC GAGACCATCA ACGATGGTCA GTTCCACACA 
GTGGAGCTGG TGACCTTCGA CCAGATGGTG AACCTGTCCA TTGATGGGGG TCTCCCTACC 
ACAATGGACA GCTTTGGGGC TGTGCGGCCC CTCAACGGAG AGGCGCCACT ATACGTGGGG 
GGTAGGGGCC CTCTCCAGAA CACACCTCCC TCCTCTGCAG GCATGCCTGT GGATGTCCAC 
TCCGGCACGT TCCGTCTCTG GCAGATCCAG AACGGCTCCA GCTTCCACGG CTGCATCCAG 
AACCTGTACA TCAACAATGA GCTCCAGGAC TTCACCAAGA CCCAAATGAA GCCCGGTGTG 
GTGCCAGGCT GCGAGCCCTG CAAGAAGCTC TACTGTGTCC ATGGCATCTG CCAGCCTGAC 
GGGTTACAGG GACCCGTGTG TCACTGCCAG CCAGGCTGGA GCGGGGCGCA CTGTGATCAG 
CCGGCCCTCA ACCCCTGCCA GGGCAGCAAA TGCGTCCATG GAAAGTGCAT TCCTCTGGAC 
TCTCAGTCTT ACCGCTGCGA GTGCGAAGAG GGCTACAGAG GCGCGCTTTG TAACCAGCAA 
GGAGAGCTGT TCAACCCCTG CCGCCGCCTC TCCTGCAAAC ACGGCCGCTG CCAGATCTCC 
GACACCGGAG ACGCATACTG TCACTGCGAA AGCGGCTACA CGGGGGAACT CTGTGATGCA 
GAGTCTGAAT GTCGAGGCGA GCCGGTGAGA GACTTCTACC AGGTACAGCG GGGTTACGCC 
ATCTGCCAAA CCACCCGCAT GGTCTCCTGG GTGGAGTGCA CCGGAGCTTG TGACACAGGG 
AGCTGCTGCA TCAGCCAGAG GATGAAGCGC CGGAAATACA CCTTTGAGTG CAGCGACGGA 
ACGTCCTTCA CCGAGGAGGT GGAAAAGACC ATCAAGTGCG GCTGCGTGGG CTGCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012735925.1
CDS	109..5088
Translation	MQTRANTNSTKTRQERSFQEGKKERKKETIPFFFTWTNYSGYQSCIIRSVGFVWKIFFFFLPSIYTLQLNKIVPAGLVRCGRTFLEAKRKENIKRGGQKQVRRGKKKKKRLRNMPAEDSSFESSMGRLAGQWMWLLMLSALMLGDASACPALCTCSGTTVDCHGLGLKTMPRSIPRNTERLELNGNNLTRITKSDFAGLKYLRVLQLMENQITVIERGAFDDMKELERLRLNRNQLQQLPELLFQKNPGLSRLDLSENFIQSIPRKAFRGATDIKNLQLDKNHIGCIEDGAFRALRGLEVLTLNNNNISSIPVSSFNHMPKLRTFRLHSNNLNCDCHLAWLSQWLRQRPTIGLFTQCASPPELRGLNVAEVQKHEFSCSGHQESSSLQPCSIGGGSCPAMCTCSNNIVDCRGKGLTAIPANLPDSMSEIRLEQNGIKSVPPGAFSPYKKLRRIDLSNNQISEIAPDAFQGLRSLNSLVLYGNKITDLPKGVFDGLYSLQLLLLNANKIHCVRANAFQDLQNLSLLSLYDNKIQTLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADYLRSNPIETSGARCASPRRLANKRIGQIKSKKFRCSAKEQYVIPGTEDTRLNNACNTDPVCPPKCRCESNVVDCSNLKLTKIPEHIPASTAELRLNNNEITSLEATGVFKNLSHLKKINLSNNKITEIEDGAFEGASSVIELHLTANQIDSVRSGMFRGLEGLRMMMLRNNKISCVHNDSFTGLHNVRLLSLYDNHLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTGNPRCQRPAFLKEIPLQDVALPDFRCEEGQEESSCVPRPQCPSECTCLETVVRCSNKHLHTLPKGIPRNVTELYLDGNQFSVVPKELSSFKFLQLVDLSNNKINSLTNSSFANMSQLTTLILSYNSLRCIPKMAFSGLHSLRLLSLHGNEISELPDGIFNDVTSLSHLAIGANPLHCDCHLRWLSDWVKTGYKEPGIARCAGPQGMESKLLLTTPAKKFECTGDVDTSVLAKCNPCLSSPCLNHGICHNDPLEIYRCSCPPGFKGKNCETAANACVSSPCANGGTCKVNEEDEGEYRCSCLPGFEGPTCQINVDDCEDNDCENGATCIDGVNNYTCFCPPYYTGEMCEEMDDVCAPGRSPCQHQSTCLISTSGPKCVCSPGYVGDDCSVNYDDCREHRCQNGAQCVDELNGYSCICPEGYSGQLCEVPPSPLSLCELADCQNNAPCVERGGRALCQCPPEFGGPRCEKLVSVNFVDRDSYLLLSDLENWPQANITLQVSTAEDNGILLYNGDNDHIAVELFQGHVKVSYDPGSQPGHAIYSTETINDGQFHTVELVTFDQMVNLSIDGGLPTTMDSFGAVRPLNGEAPLYVGGRGPLQNTPPSSAGMPVDVHSGTFRLWQIQNGSSFHGCIQNLYINNELQDFTKTQMKPGVVPGCEPCKKLYCVHGICQPDGLQGPVCHCQPGWSGAHCDQPALNPCQGSKCVHGKCIPLDSQSYRCECEEGYRGALCNQQGELFNPCRRLSCKHGRCQISDTGDAYCHCESGYTGELCDAESECRGEPVRDFYQVQRGYAICQTTRMVSWVECTGACDTGSCCISQRMKRRKYTFECSDGTSFTEEVEKTIKCGCVGCM

Target ORF information:

RefSeq Version	XM_012880471.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus slit homolog 1 (Drosophila) (slit1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012880471.1

ORF Insert Sequence:

ATGCAGACCA GAGCAAACAC CAACTCCACA AAGACGAGAC AAGAGAGGAG TTTTCAAGAA 
GGAAAGAAAG AAAGAAAGAA AGAAACCATC CCTTTTTTTT TTACATGGAC AAATTATAGT 
GGATATCAAA GCTGCATCAT CAGATCTGTA GGATTTGTGT GGAAGATCTT TTTTTTTTTT 
CTCCCTTCTA TTTATACTCT CCAGTTAAAT AAAATTGTTC CTGCAGGACT TGTGAGGTGT 
GGCAGGACTT TTTTGGAAGC CAAAAGGAAG GAGAACATAA AGAGAGGTGG ACAGAAACAA 
GTAAGGAGGG GAAAAAAAAA GAAGAAAAGG CTTAGGAACA TGCCCGCTGA AGACAGCAGT 
TTTGAATCAA GCATGGGCAG GCTGGCTGGG CAGTGGATGT GGCTGCTGAT GCTCTCTGCC 
TTGATGCTGG GCGATGCCAG CGCCTGCCCG GCGCTTTGTA CCTGCAGCGG CACCACAGTG 
GACTGCCACG GACTGGGGCT CAAAACTATG CCAAGGAGTA TCCCCCGCAA CACTGAGAGA 
CTGGAGCTGA ACGGAAACAA CCTCACACGC ATCACCAAGA GTGACTTTGC TGGCCTGAAG 
TACCTCAGAG TGCTACAGCT GATGGAGAAT CAGATTACAG TGATTGAGCG AGGAGCTTTC 
GATGACATGA AGGAGCTGGA GCGGCTGCGG CTGAACCGTA ATCAACTGCA GCAGCTTCCG 
GAGCTGCTGT TTCAGAAGAA CCCCGGCCTG TCTCGGCTCG ATCTGAGTGA GAACTTCATC 
CAATCCATCC CCAGAAAAGC CTTCAGAGGA GCGACAGACA TCAAAAACCT GCAGCTGGAT 
AAAAACCACA TCGGCTGCAT CGAGGATGGA GCCTTCCGAG CTCTAAGAGG CCTGGAAGTA 
CTAACGCTGA ACAACAACAA CATCAGCAGC ATCCCCGTCT CCAGCTTCAA CCACATGCCC 
AAACTCCGAA CTTTCCGTCT TCACTCCAAC AACTTAAACT GTGATTGTCA CCTGGCCTGG 
CTGTCCCAGT GGCTCAGACA GAGGCCTACG ATTGGTCTGT TCACCCAGTG TGCTTCGCCG 
CCAGAGCTAA GAGGACTCAA TGTGGCGGAG GTCCAGAAAC ACGAATTCTC CTGTTCAGGG 
CATCAGGAGT CTTCCAGCCT GCAGCCGTGC AGCATTGGCG GAGGCTCATG TCCCGCCATG 
TGCACCTGCA GTAACAACAT CGTGGACTGC AGAGGGAAAG GTCTCACTGC CATTCCAGCC 
AATCTGCCGG ACAGCATGTC AGAAATACGT CTGGAGCAGA ACGGGATTAA GTCTGTGCCT 
CCCGGAGCCT TCTCCCCCTA CAAGAAACTG CGCAGGATAG ACCTGAGCAA CAACCAGATC 
TCAGAGATAG CGCCTGACGC CTTCCAGGGA CTGCGATCTC TTAACTCTCT CGTGCTTTAC 
GGAAACAAGA TCACCGACCT GCCGAAGGGT GTGTTTGACG GCCTCTACTC TTTGCAACTG 
CTGCTGCTGA ATGCCAATAA GATTCACTGC GTCCGTGCCA ATGCCTTCCA GGATCTGCAG 
AATCTCTCCC TGCTATCGCT GTACGATAAC AAGATCCAAA CCCTCGCCAA GGGCACCTTC 
ACTTCCCTGC GAGCAATACA AACCCTGCAC CTGGCGCAGA ACCCGTTTAT TTGTGACTGT 
AATCTGAAGT GGCTGGCAGA CTACCTGCGC TCCAACCCAA TCGAGACCAG CGGCGCCCGC 
TGCGCCAGCC CACGGCGCCT CGCAAACAAA CGCATCGGAC AAATCAAGAG CAAGAAGTTC 
CGCTGCTCCG CCAAAGAGCA GTACGTCATC CCAGGTACAG AAGACACCCG TCTCAATAAC 
GCCTGCAACA CTGATCCCGT GTGTCCTCCC AAGTGTCGCT GTGAGTCTAA CGTAGTGGAC 
TGCTCCAACC TGAAACTTAC CAAGATCCCC GAGCACATCC CTGCATCCAC AGCTGAACTC 
CGCCTTAACA ATAACGAGAT CACCAGCCTT GAGGCAACTG GAGTCTTTAA GAACCTTTCC 
CATCTGAAGA AAATTAACCT GAGTAACAAC AAGATCACAG AGATCGAGGA CGGGGCTTTT 
GAAGGTGCAT CCTCTGTCAT TGAGCTCCAC CTCACAGCCA ATCAAATCGA CTCGGTTCGC 
AGCGGGATGT TCCGAGGCCT CGAGGGACTG CGCATGATGA TGCTGCGGAA CAACAAAATC 
AGCTGTGTCC ACAACGACAG CTTCACGGGG CTTCACAACG TCCGTCTGCT GTCTCTGTAC 
GACAACCACC TGACCACCAT TACTCCTGGA GCATTCGACA CGCTGCAGAC TCTCTCTACT 
CTAAACCTCT TGGCCAACTC CTTCAACTGC GACTGCCGGC TGGCGTGGCT CGGCGACTGG 
CTGAGGAGCA GGAAGATTGT GACGGGTAAC CCTCGCTGCC AGCGCCCGGC CTTCCTGAAG 
GAGATCCCCC TGCAGGATGT AGCGCTGCCG GATTTTCGCT GCGAGGAAGG CCAAGAGGAG 
TCAAGCTGTG TTCCTCGACC CCAGTGTCCC AGCGAGTGTA CTTGCCTAGA GACGGTGGTG 
CGCTGCAGCA ACAAGCATCT CCACACACTA CCCAAAGGCA TCCCCAGGAA TGTGACTGAA 
CTGTACCTTG ATGGCAACCA GTTCAGTGTT GTTCCCAAGG AACTGTCTTC CTTCAAATTC 
CTACAGCTGG TTGACCTGAG CAACAACAAA ATCAACTCTC TGACCAATTC ATCCTTTGCT 
AACATGAGCC AACTGACCAC TCTAATCCTG AGCTACAACT CCCTGCGCTG CATACCCAAA 
ATGGCTTTCA GTGGCCTGCA CTCACTGCGA CTGCTGTCAC TTCATGGTAA TGAAATCTCA 
GAGCTTCCAG ATGGCATCTT CAATGATGTG ACCTCCCTCT CCCATTTGGC GATCGGAGCC 
AACCCTCTGC ACTGTGACTG CCACCTGCGC TGGCTCTCCG ACTGGGTGAA GACCGGATAC 
AAAGAACCTG GGATCGCGCG CTGTGCCGGC CCACAGGGGA TGGAGAGCAA GCTGCTCCTC 
ACCACGCCTG CCAAGAAATT TGAATGCACA GGCGATGTGG ACACGTCGGT GCTGGCCAAG 
TGTAACCCCT GCCTGTCCAG TCCCTGCCTG AATCACGGCA TCTGTCACAA TGACCCATTG 
GAGATCTACA GGTGCAGCTG TCCTCCTGGG TTCAAGGGCA AGAACTGTGA GACGGCTGCG 
AATGCCTGTG TGAGTAGCCC ATGCGCCAAT GGAGGAACCT GCAAAGTGAA TGAGGAGGAC 
GAGGGAGAGT ACCGCTGCTC CTGTCTGCCG GGCTTCGAGG GCCCGACTTG CCAAATCAAT 
GTTGACGACT GTGAAGACAA CGACTGTGAG AACGGGGCGA CCTGCATTGA TGGAGTCAAC 
AACTACACTT GTTTTTGTCC CCCCTACTAC ACAGGGGAGA TGTGTGAAGA AATGGACGAT 
GTGTGTGCTC CAGGGCGAAG CCCCTGCCAA CATCAGTCTA CCTGCCTCAT CAGCACCTCT 
GGACCCAAGT GTGTGTGTTC TCCTGGGTAT GTTGGTGATG ACTGCAGTGT CAACTACGAT 
GACTGCAGGG AGCATCGCTG TCAGAACGGA GCACAGTGCG TGGATGAGCT GAACGGATAT 
TCCTGCATCT GCCCCGAAGG ATATAGCGGC CAGCTGTGTG AAGTTCCCCC ATCTCCCCTG 
TCACTGTGTG AGCTGGCCGA CTGCCAAAAC AACGCCCCCT GTGTGGAGCG AGGTGGCCGC 
GCCCTCTGCC AGTGTCCTCC AGAGTTCGGG GGTCCGCGCT GCGAAAAACT GGTCAGCGTC 
AACTTTGTCG ACAGAGACTC CTACTTATTG CTGTCCGACC TGGAGAACTG GCCACAAGCT 
AACATCACCC TGCAGGTTTC CACAGCTGAG GACAACGGGA TCCTGCTGTA CAATGGAGAC 
AACGATCACA TTGCAGTGGA GCTCTTCCAA GGCCACGTGA AAGTGAGCTA CGACCCGGGC 
AGCCAGCCAG GACACGCCAT ATACAGCACC GAGACCATCA ACGATGGTCA GTTCCACACA 
GTGGAGCTGG TGACCTTCGA CCAGATGGTG AACCTGTCCA TTGATGGGGG TCTCCCTACC 
ACAATGGACA GCTTTGGGGC TGTGCGGCCC CTCAACGGAG AGGCGCCACT ATACGTGGGG 
GGTAGGGGCC CTCTCCAGAA CACACCTCCC TCCTCTGCAG GCATGCCTGT GGATGTCCAC 
TCCGGCACGT TCCGTCTCTG GCAGATCCAG AACGGCTCCA GCTTCCACGG CTGCATCCAG 
AACCTGTACA TCAACAATGA GCTCCAGGAC TTCACCAAGA CCCAAATGAA GCCCGGTGTG 
GTGCCAGGCT GCGAGCCCTG CAAGAAGCTC TACTGTGTCC ATGGCATCTG CCAGCCTGAC 
GGGTTACAGG GACCCGTGTG TCACTGCCAG CCAGGCTGGA GCGGGGCGCA CTGTGATCAG 
CCGGCCCTCA ACCCCTGCCA GGGCAGCAAA TGCGTCCATG GAAAGTGCAT TCCTCTGGAC 
TCTCAGTCTT ACCGCTGCGA GTGCGAAGAG GGCTACAGAG GCGCGCTTTG TAACCAGCAA 
GGAGAGCTGT TCAACCCCTG CCGCCGCCTC TCCTGCAAAC ACGGCCGCTG CCAGATCTCC 
GACACCGGAG ACGCATACTG TCACTGCGAA AGCGGCTACA CGGGGGAACT CTGTGATGCA 
GAGTCTGAAT GTCGAGGCGA GCCGGTGAGA GACTTCTACC AGGTACAGCG GGGTTACGCC 
ATCTGCCAAA CCACCCGCAT GGTCTCCTGG GTGGAGTGCA CCGGAGCTTG TGACACAGGG 
AGCTGCTGCA TCAGCCAGAG GATGAAGCGC CGGAAATACA CCTTTGAGTG CAGCGACGGA 
ACGTCCTTCA CCGAGGAGGT GGAAAAGACC ATCAAGTGCG GCTGCGTGGG CTGCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105938657 ( XM_012880471.2 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012735925.1 Fundulus heteroclitus slit homolog 1 protein (LOC105938657), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFu124012
Clone ID Related Accession (Same CDS sequence)	XM_012880471.1 , XM_012880471.2
Accession Version	XM_012880471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	slit homolog 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234518.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 1, 2017 this sequence version replaced XM_012880471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA GAGCAAACAC CAACTCCACA AAGACGAGAC AAGAGAGGAG TTTTCAAGAA 
GGAAAGAAAG AAAGAAAGAA AGAAACCATC CCTTTTTTTT TTACATGGAC AAATTATAGT 
GGATATCAAA GCTGCATCAT CAGATCTGTA GGATTTGTGT GGAAGATCTT TTTTTTTTTT 
CTCCCTTCTA TTTATACTCT CCAGTTAAAT AAAATTGTTC CTGCAGGACT TGTGAGGTGT 
GGCAGGACTT TTTTGGAAGC CAAAAGGAAG GAGAACATAA AGAGAGGTGG ACAGAAACAA 
GTAAGGAGGG GAAAAAAAAA GAAGAAAAGG CTTAGGAACA TGCCCGCTGA AGACAGCAGT 
TTTGAATCAA GCATGGGCAG GCTGGCTGGG CAGTGGATGT GGCTGCTGAT GCTCTCTGCC 
TTGATGCTGG GCGATGCCAG CGCCTGCCCG GCGCTTTGTA CCTGCAGCGG CACCACAGTG 
GACTGCCACG GACTGGGGCT CAAAACTATG CCAAGGAGTA TCCCCCGCAA CACTGAGAGA 
CTGGAGCTGA ACGGAAACAA CCTCACACGC ATCACCAAGA GTGACTTTGC TGGCCTGAAG 
TACCTCAGAG TGCTACAGCT GATGGAGAAT CAGATTACAG TGATTGAGCG AGGAGCTTTC 
GATGACATGA AGGAGCTGGA GCGGCTGCGG CTGAACCGTA ATCAACTGCA GCAGCTTCCG 
GAGCTGCTGT TTCAGAAGAA CCCCGGCCTG TCTCGGCTCG ATCTGAGTGA GAACTTCATC 
CAATCCATCC CCAGAAAAGC CTTCAGAGGA GCGACAGACA TCAAAAACCT GCAGCTGGAT 
AAAAACCACA TCGGCTGCAT CGAGGATGGA GCCTTCCGAG CTCTAAGAGG CCTGGAAGTA 
CTAACGCTGA ACAACAACAA CATCAGCAGC ATCCCCGTCT CCAGCTTCAA CCACATGCCC 
AAACTCCGAA CTTTCCGTCT TCACTCCAAC AACTTAAACT GTGATTGTCA CCTGGCCTGG 
CTGTCCCAGT GGCTCAGACA GAGGCCTACG ATTGGTCTGT TCACCCAGTG TGCTTCGCCG 
CCAGAGCTAA GAGGACTCAA TGTGGCGGAG GTCCAGAAAC ACGAATTCTC CTGTTCAGGG 
CATCAGGAGT CTTCCAGCCT GCAGCCGTGC AGCATTGGCG GAGGCTCATG TCCCGCCATG 
TGCACCTGCA GTAACAACAT CGTGGACTGC AGAGGGAAAG GTCTCACTGC CATTCCAGCC 
AATCTGCCGG ACAGCATGTC AGAAATACGT CTGGAGCAGA ACGGGATTAA GTCTGTGCCT 
CCCGGAGCCT TCTCCCCCTA CAAGAAACTG CGCAGGATAG ACCTGAGCAA CAACCAGATC 
TCAGAGATAG CGCCTGACGC CTTCCAGGGA CTGCGATCTC TTAACTCTCT CGTGCTTTAC 
GGAAACAAGA TCACCGACCT GCCGAAGGGT GTGTTTGACG GCCTCTACTC TTTGCAACTG 
CTGCTGCTGA ATGCCAATAA GATTCACTGC GTCCGTGCCA ATGCCTTCCA GGATCTGCAG 
AATCTCTCCC TGCTATCGCT GTACGATAAC AAGATCCAAA CCCTCGCCAA GGGCACCTTC 
ACTTCCCTGC GAGCAATACA AACCCTGCAC CTGGCGCAGA ACCCGTTTAT TTGTGACTGT 
AATCTGAAGT GGCTGGCAGA CTACCTGCGC TCCAACCCAA TCGAGACCAG CGGCGCCCGC 
TGCGCCAGCC CACGGCGCCT CGCAAACAAA CGCATCGGAC AAATCAAGAG CAAGAAGTTC 
CGCTGCTCCG CCAAAGAGCA GTACGTCATC CCAGGTACAG AAGACACCCG TCTCAATAAC 
GCCTGCAACA CTGATCCCGT GTGTCCTCCC AAGTGTCGCT GTGAGTCTAA CGTAGTGGAC 
TGCTCCAACC TGAAACTTAC CAAGATCCCC GAGCACATCC CTGCATCCAC AGCTGAACTC 
CGCCTTAACA ATAACGAGAT CACCAGCCTT GAGGCAACTG GAGTCTTTAA GAACCTTTCC 
CATCTGAAGA AAATTAACCT GAGTAACAAC AAGATCACAG AGATCGAGGA CGGGGCTTTT 
GAAGGTGCAT CCTCTGTCAT TGAGCTCCAC CTCACAGCCA ATCAAATCGA CTCGGTTCGC 
AGCGGGATGT TCCGAGGCCT CGAGGGACTG CGCATGATGA TGCTGCGGAA CAACAAAATC 
AGCTGTGTCC ACAACGACAG CTTCACGGGG CTTCACAACG TCCGTCTGCT GTCTCTGTAC 
GACAACCACC TGACCACCAT TACTCCTGGA GCATTCGACA CGCTGCAGAC TCTCTCTACT 
CTAAACCTCT TGGCCAACTC CTTCAACTGC GACTGCCGGC TGGCGTGGCT CGGCGACTGG 
CTGAGGAGCA GGAAGATTGT GACGGGTAAC CCTCGCTGCC AGCGCCCGGC CTTCCTGAAG 
GAGATCCCCC TGCAGGATGT AGCGCTGCCG GATTTTCGCT GCGAGGAAGG CCAAGAGGAG 
TCAAGCTGTG TTCCTCGACC CCAGTGTCCC AGCGAGTGTA CTTGCCTAGA GACGGTGGTG 
CGCTGCAGCA ACAAGCATCT CCACACACTA CCCAAAGGCA TCCCCAGGAA TGTGACTGAA 
CTGTACCTTG ATGGCAACCA GTTCAGTGTT GTTCCCAAGG AACTGTCTTC CTTCAAATTC 
CTACAGCTGG TTGACCTGAG CAACAACAAA ATCAACTCTC TGACCAATTC ATCCTTTGCT 
AACATGAGCC AACTGACCAC TCTAATCCTG AGCTACAACT CCCTGCGCTG CATACCCAAA 
ATGGCTTTCA GTGGCCTGCA CTCACTGCGA CTGCTGTCAC TTCATGGTAA TGAAATCTCA 
GAGCTTCCAG ATGGCATCTT CAATGATGTG ACCTCCCTCT CCCATTTGGC GATCGGAGCC 
AACCCTCTGC ACTGTGACTG CCACCTGCGC TGGCTCTCCG ACTGGGTGAA GACCGGATAC 
AAAGAACCTG GGATCGCGCG CTGTGCCGGC CCACAGGGGA TGGAGAGCAA GCTGCTCCTC 
ACCACGCCTG CCAAGAAATT TGAATGCACA GGCGATGTGG ACACGTCGGT GCTGGCCAAG 
TGTAACCCCT GCCTGTCCAG TCCCTGCCTG AATCACGGCA TCTGTCACAA TGACCCATTG 
GAGATCTACA GGTGCAGCTG TCCTCCTGGG TTCAAGGGCA AGAACTGTGA GACGGCTGCG 
AATGCCTGTG TGAGTAGCCC ATGCGCCAAT GGAGGAACCT GCAAAGTGAA TGAGGAGGAC 
GAGGGAGAGT ACCGCTGCTC CTGTCTGCCG GGCTTCGAGG GCCCGACTTG CCAAATCAAT 
GTTGACGACT GTGAAGACAA CGACTGTGAG AACGGGGCGA CCTGCATTGA TGGAGTCAAC 
AACTACACTT GTTTTTGTCC CCCCTACTAC ACAGGGGAGA TGTGTGAAGA AATGGACGAT 
GTGTGTGCTC CAGGGCGAAG CCCCTGCCAA CATCAGTCTA CCTGCCTCAT CAGCACCTCT 
GGACCCAAGT GTGTGTGTTC TCCTGGGTAT GTTGGTGATG ACTGCAGTGT CAACTACGAT 
GACTGCAGGG AGCATCGCTG TCAGAACGGA GCACAGTGCG TGGATGAGCT GAACGGATAT 
TCCTGCATCT GCCCCGAAGG ATATAGCGGC CAGCTGTGTG AAGTTCCCCC ATCTCCCCTG 
TCACTGTGTG AGCTGGCCGA CTGCCAAAAC AACGCCCCCT GTGTGGAGCG AGGTGGCCGC 
GCCCTCTGCC AGTGTCCTCC AGAGTTCGGG GGTCCGCGCT GCGAAAAACT GGTCAGCGTC 
AACTTTGTCG ACAGAGACTC CTACTTATTG CTGTCCGACC TGGAGAACTG GCCACAAGCT 
AACATCACCC TGCAGGTTTC CACAGCTGAG GACAACGGGA TCCTGCTGTA CAATGGAGAC 
AACGATCACA TTGCAGTGGA GCTCTTCCAA GGCCACGTGA AAGTGAGCTA CGACCCGGGC 
AGCCAGCCAG GACACGCCAT ATACAGCACC GAGACCATCA ACGATGGTCA GTTCCACACA 
GTGGAGCTGG TGACCTTCGA CCAGATGGTG AACCTGTCCA TTGATGGGGG TCTCCCTACC 
ACAATGGACA GCTTTGGGGC TGTGCGGCCC CTCAACGGAG AGGCGCCACT ATACGTGGGG 
GGTAGGGGCC CTCTCCAGAA CACACCTCCC TCCTCTGCAG GCATGCCTGT GGATGTCCAC 
TCCGGCACGT TCCGTCTCTG GCAGATCCAG AACGGCTCCA GCTTCCACGG CTGCATCCAG 
AACCTGTACA TCAACAATGA GCTCCAGGAC TTCACCAAGA CCCAAATGAA GCCCGGTGTG 
GTGCCAGGCT GCGAGCCCTG CAAGAAGCTC TACTGTGTCC ATGGCATCTG CCAGCCTGAC 
GGGTTACAGG GACCCGTGTG TCACTGCCAG CCAGGCTGGA GCGGGGCGCA CTGTGATCAG 
CCGGCCCTCA ACCCCTGCCA GGGCAGCAAA TGCGTCCATG GAAAGTGCAT TCCTCTGGAC 
TCTCAGTCTT ACCGCTGCGA GTGCGAAGAG GGCTACAGAG GCGCGCTTTG TAACCAGCAA 
GGAGAGCTGT TCAACCCCTG CCGCCGCCTC TCCTGCAAAC ACGGCCGCTG CCAGATCTCC 
GACACCGGAG ACGCATACTG TCACTGCGAA AGCGGCTACA CGGGGGAACT CTGTGATGCA 
GAGTCTGAAT GTCGAGGCGA GCCGGTGAGA GACTTCTACC AGGTACAGCG GGGTTACGCC 
ATCTGCCAAA CCACCCGCAT GGTCTCCTGG GTGGAGTGCA CCGGAGCTTG TGACACAGGG 
AGCTGCTGCA TCAGCCAGAG GATGAAGCGC CGGAAATACA CCTTTGAGTG CAGCGACGGA 
ACGTCCTTCA CCGAGGAGGT GGAAAAGACC ATCAAGTGCG GCTGCGTGGG CTGCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012735925.1
CDS	30..5009
Translation	MQTRANTNSTKTRQERSFQEGKKERKKETIPFFFTWTNYSGYQSCIIRSVGFVWKIFFFFLPSIYTLQLNKIVPAGLVRCGRTFLEAKRKENIKRGGQKQVRRGKKKKKRLRNMPAEDSSFESSMGRLAGQWMWLLMLSALMLGDASACPALCTCSGTTVDCHGLGLKTMPRSIPRNTERLELNGNNLTRITKSDFAGLKYLRVLQLMENQITVIERGAFDDMKELERLRLNRNQLQQLPELLFQKNPGLSRLDLSENFIQSIPRKAFRGATDIKNLQLDKNHIGCIEDGAFRALRGLEVLTLNNNNISSIPVSSFNHMPKLRTFRLHSNNLNCDCHLAWLSQWLRQRPTIGLFTQCASPPELRGLNVAEVQKHEFSCSGHQESSSLQPCSIGGGSCPAMCTCSNNIVDCRGKGLTAIPANLPDSMSEIRLEQNGIKSVPPGAFSPYKKLRRIDLSNNQISEIAPDAFQGLRSLNSLVLYGNKITDLPKGVFDGLYSLQLLLLNANKIHCVRANAFQDLQNLSLLSLYDNKIQTLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADYLRSNPIETSGARCASPRRLANKRIGQIKSKKFRCSAKEQYVIPGTEDTRLNNACNTDPVCPPKCRCESNVVDCSNLKLTKIPEHIPASTAELRLNNNEITSLEATGVFKNLSHLKKINLSNNKITEIEDGAFEGASSVIELHLTANQIDSVRSGMFRGLEGLRMMMLRNNKISCVHNDSFTGLHNVRLLSLYDNHLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTGNPRCQRPAFLKEIPLQDVALPDFRCEEGQEESSCVPRPQCPSECTCLETVVRCSNKHLHTLPKGIPRNVTELYLDGNQFSVVPKELSSFKFLQLVDLSNNKINSLTNSSFANMSQLTTLILSYNSLRCIPKMAFSGLHSLRLLSLHGNEISELPDGIFNDVTSLSHLAIGANPLHCDCHLRWLSDWVKTGYKEPGIARCAGPQGMESKLLLTTPAKKFECTGDVDTSVLAKCNPCLSSPCLNHGICHNDPLEIYRCSCPPGFKGKNCETAANACVSSPCANGGTCKVNEEDEGEYRCSCLPGFEGPTCQINVDDCEDNDCENGATCIDGVNNYTCFCPPYYTGEMCEEMDDVCAPGRSPCQHQSTCLISTSGPKCVCSPGYVGDDCSVNYDDCREHRCQNGAQCVDELNGYSCICPEGYSGQLCEVPPSPLSLCELADCQNNAPCVERGGRALCQCPPEFGGPRCEKLVSVNFVDRDSYLLLSDLENWPQANITLQVSTAEDNGILLYNGDNDHIAVELFQGHVKVSYDPGSQPGHAIYSTETINDGQFHTVELVTFDQMVNLSIDGGLPTTMDSFGAVRPLNGEAPLYVGGRGPLQNTPPSSAGMPVDVHSGTFRLWQIQNGSSFHGCIQNLYINNELQDFTKTQMKPGVVPGCEPCKKLYCVHGICQPDGLQGPVCHCQPGWSGAHCDQPALNPCQGSKCVHGKCIPLDSQSYRCECEEGYRGALCNQQGELFNPCRRLSCKHGRCQISDTGDAYCHCESGYTGELCDAESECRGEPVRDFYQVQRGYAICQTTRMVSWVECTGACDTGSCCISQRMKRRKYTFECSDGTSFTEEVEKTIKCGCVGCM

Target ORF information:

RefSeq Version	XM_012880471.2
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus slit homolog 1 protein (LOC105938657), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012880471.2

ORF Insert Sequence:

ATGCAGACCA GAGCAAACAC CAACTCCACA AAGACGAGAC AAGAGAGGAG TTTTCAAGAA 
GGAAAGAAAG AAAGAAAGAA AGAAACCATC CCTTTTTTTT TTACATGGAC AAATTATAGT 
GGATATCAAA GCTGCATCAT CAGATCTGTA GGATTTGTGT GGAAGATCTT TTTTTTTTTT 
CTCCCTTCTA TTTATACTCT CCAGTTAAAT AAAATTGTTC CTGCAGGACT TGTGAGGTGT 
GGCAGGACTT TTTTGGAAGC CAAAAGGAAG GAGAACATAA AGAGAGGTGG ACAGAAACAA 
GTAAGGAGGG GAAAAAAAAA GAAGAAAAGG CTTAGGAACA TGCCCGCTGA AGACAGCAGT 
TTTGAATCAA GCATGGGCAG GCTGGCTGGG CAGTGGATGT GGCTGCTGAT GCTCTCTGCC 
TTGATGCTGG GCGATGCCAG CGCCTGCCCG GCGCTTTGTA CCTGCAGCGG CACCACAGTG 
GACTGCCACG GACTGGGGCT CAAAACTATG CCAAGGAGTA TCCCCCGCAA CACTGAGAGA 
CTGGAGCTGA ACGGAAACAA CCTCACACGC ATCACCAAGA GTGACTTTGC TGGCCTGAAG 
TACCTCAGAG TGCTACAGCT GATGGAGAAT CAGATTACAG TGATTGAGCG AGGAGCTTTC 
GATGACATGA AGGAGCTGGA GCGGCTGCGG CTGAACCGTA ATCAACTGCA GCAGCTTCCG 
GAGCTGCTGT TTCAGAAGAA CCCCGGCCTG TCTCGGCTCG ATCTGAGTGA GAACTTCATC 
CAATCCATCC CCAGAAAAGC CTTCAGAGGA GCGACAGACA TCAAAAACCT GCAGCTGGAT 
AAAAACCACA TCGGCTGCAT CGAGGATGGA GCCTTCCGAG CTCTAAGAGG CCTGGAAGTA 
CTAACGCTGA ACAACAACAA CATCAGCAGC ATCCCCGTCT CCAGCTTCAA CCACATGCCC 
AAACTCCGAA CTTTCCGTCT TCACTCCAAC AACTTAAACT GTGATTGTCA CCTGGCCTGG 
CTGTCCCAGT GGCTCAGACA GAGGCCTACG ATTGGTCTGT TCACCCAGTG TGCTTCGCCG 
CCAGAGCTAA GAGGACTCAA TGTGGCGGAG GTCCAGAAAC ACGAATTCTC CTGTTCAGGG 
CATCAGGAGT CTTCCAGCCT GCAGCCGTGC AGCATTGGCG GAGGCTCATG TCCCGCCATG 
TGCACCTGCA GTAACAACAT CGTGGACTGC AGAGGGAAAG GTCTCACTGC CATTCCAGCC 
AATCTGCCGG ACAGCATGTC AGAAATACGT CTGGAGCAGA ACGGGATTAA GTCTGTGCCT 
CCCGGAGCCT TCTCCCCCTA CAAGAAACTG CGCAGGATAG ACCTGAGCAA CAACCAGATC 
TCAGAGATAG CGCCTGACGC CTTCCAGGGA CTGCGATCTC TTAACTCTCT CGTGCTTTAC 
GGAAACAAGA TCACCGACCT GCCGAAGGGT GTGTTTGACG GCCTCTACTC TTTGCAACTG 
CTGCTGCTGA ATGCCAATAA GATTCACTGC GTCCGTGCCA ATGCCTTCCA GGATCTGCAG 
AATCTCTCCC TGCTATCGCT GTACGATAAC AAGATCCAAA CCCTCGCCAA GGGCACCTTC 
ACTTCCCTGC GAGCAATACA AACCCTGCAC CTGGCGCAGA ACCCGTTTAT TTGTGACTGT 
AATCTGAAGT GGCTGGCAGA CTACCTGCGC TCCAACCCAA TCGAGACCAG CGGCGCCCGC 
TGCGCCAGCC CACGGCGCCT CGCAAACAAA CGCATCGGAC AAATCAAGAG CAAGAAGTTC 
CGCTGCTCCG CCAAAGAGCA GTACGTCATC CCAGGTACAG AAGACACCCG TCTCAATAAC 
GCCTGCAACA CTGATCCCGT GTGTCCTCCC AAGTGTCGCT GTGAGTCTAA CGTAGTGGAC 
TGCTCCAACC TGAAACTTAC CAAGATCCCC GAGCACATCC CTGCATCCAC AGCTGAACTC 
CGCCTTAACA ATAACGAGAT CACCAGCCTT GAGGCAACTG GAGTCTTTAA GAACCTTTCC 
CATCTGAAGA AAATTAACCT GAGTAACAAC AAGATCACAG AGATCGAGGA CGGGGCTTTT 
GAAGGTGCAT CCTCTGTCAT TGAGCTCCAC CTCACAGCCA ATCAAATCGA CTCGGTTCGC 
AGCGGGATGT TCCGAGGCCT CGAGGGACTG CGCATGATGA TGCTGCGGAA CAACAAAATC 
AGCTGTGTCC ACAACGACAG CTTCACGGGG CTTCACAACG TCCGTCTGCT GTCTCTGTAC 
GACAACCACC TGACCACCAT TACTCCTGGA GCATTCGACA CGCTGCAGAC TCTCTCTACT 
CTAAACCTCT TGGCCAACTC CTTCAACTGC GACTGCCGGC TGGCGTGGCT CGGCGACTGG 
CTGAGGAGCA GGAAGATTGT GACGGGTAAC CCTCGCTGCC AGCGCCCGGC CTTCCTGAAG 
GAGATCCCCC TGCAGGATGT AGCGCTGCCG GATTTTCGCT GCGAGGAAGG CCAAGAGGAG 
TCAAGCTGTG TTCCTCGACC CCAGTGTCCC AGCGAGTGTA CTTGCCTAGA GACGGTGGTG 
CGCTGCAGCA ACAAGCATCT CCACACACTA CCCAAAGGCA TCCCCAGGAA TGTGACTGAA 
CTGTACCTTG ATGGCAACCA GTTCAGTGTT GTTCCCAAGG AACTGTCTTC CTTCAAATTC 
CTACAGCTGG TTGACCTGAG CAACAACAAA ATCAACTCTC TGACCAATTC ATCCTTTGCT 
AACATGAGCC AACTGACCAC TCTAATCCTG AGCTACAACT CCCTGCGCTG CATACCCAAA 
ATGGCTTTCA GTGGCCTGCA CTCACTGCGA CTGCTGTCAC TTCATGGTAA TGAAATCTCA 
GAGCTTCCAG ATGGCATCTT CAATGATGTG ACCTCCCTCT CCCATTTGGC GATCGGAGCC 
AACCCTCTGC ACTGTGACTG CCACCTGCGC TGGCTCTCCG ACTGGGTGAA GACCGGATAC 
AAAGAACCTG GGATCGCGCG CTGTGCCGGC CCACAGGGGA TGGAGAGCAA GCTGCTCCTC 
ACCACGCCTG CCAAGAAATT TGAATGCACA GGCGATGTGG ACACGTCGGT GCTGGCCAAG 
TGTAACCCCT GCCTGTCCAG TCCCTGCCTG AATCACGGCA TCTGTCACAA TGACCCATTG 
GAGATCTACA GGTGCAGCTG TCCTCCTGGG TTCAAGGGCA AGAACTGTGA GACGGCTGCG 
AATGCCTGTG TGAGTAGCCC ATGCGCCAAT GGAGGAACCT GCAAAGTGAA TGAGGAGGAC 
GAGGGAGAGT ACCGCTGCTC CTGTCTGCCG GGCTTCGAGG GCCCGACTTG CCAAATCAAT 
GTTGACGACT GTGAAGACAA CGACTGTGAG AACGGGGCGA CCTGCATTGA TGGAGTCAAC 
AACTACACTT GTTTTTGTCC CCCCTACTAC ACAGGGGAGA TGTGTGAAGA AATGGACGAT 
GTGTGTGCTC CAGGGCGAAG CCCCTGCCAA CATCAGTCTA CCTGCCTCAT CAGCACCTCT 
GGACCCAAGT GTGTGTGTTC TCCTGGGTAT GTTGGTGATG ACTGCAGTGT CAACTACGAT 
GACTGCAGGG AGCATCGCTG TCAGAACGGA GCACAGTGCG TGGATGAGCT GAACGGATAT 
TCCTGCATCT GCCCCGAAGG ATATAGCGGC CAGCTGTGTG AAGTTCCCCC ATCTCCCCTG 
TCACTGTGTG AGCTGGCCGA CTGCCAAAAC AACGCCCCCT GTGTGGAGCG AGGTGGCCGC 
GCCCTCTGCC AGTGTCCTCC AGAGTTCGGG GGTCCGCGCT GCGAAAAACT GGTCAGCGTC 
AACTTTGTCG ACAGAGACTC CTACTTATTG CTGTCCGACC TGGAGAACTG GCCACAAGCT 
AACATCACCC TGCAGGTTTC CACAGCTGAG GACAACGGGA TCCTGCTGTA CAATGGAGAC 
AACGATCACA TTGCAGTGGA GCTCTTCCAA GGCCACGTGA AAGTGAGCTA CGACCCGGGC 
AGCCAGCCAG GACACGCCAT ATACAGCACC GAGACCATCA ACGATGGTCA GTTCCACACA 
GTGGAGCTGG TGACCTTCGA CCAGATGGTG AACCTGTCCA TTGATGGGGG TCTCCCTACC 
ACAATGGACA GCTTTGGGGC TGTGCGGCCC CTCAACGGAG AGGCGCCACT ATACGTGGGG 
GGTAGGGGCC CTCTCCAGAA CACACCTCCC TCCTCTGCAG GCATGCCTGT GGATGTCCAC 
TCCGGCACGT TCCGTCTCTG GCAGATCCAG AACGGCTCCA GCTTCCACGG CTGCATCCAG 
AACCTGTACA TCAACAATGA GCTCCAGGAC TTCACCAAGA CCCAAATGAA GCCCGGTGTG 
GTGCCAGGCT GCGAGCCCTG CAAGAAGCTC TACTGTGTCC ATGGCATCTG CCAGCCTGAC 
GGGTTACAGG GACCCGTGTG TCACTGCCAG CCAGGCTGGA GCGGGGCGCA CTGTGATCAG 
CCGGCCCTCA ACCCCTGCCA GGGCAGCAAA TGCGTCCATG GAAAGTGCAT TCCTCTGGAC 
TCTCAGTCTT ACCGCTGCGA GTGCGAAGAG GGCTACAGAG GCGCGCTTTG TAACCAGCAA 
GGAGAGCTGT TCAACCCCTG CCGCCGCCTC TCCTGCAAAC ACGGCCGCTG CCAGATCTCC 
GACACCGGAG ACGCATACTG TCACTGCGAA AGCGGCTACA CGGGGGAACT CTGTGATGCA 
GAGTCTGAAT GTCGAGGCGA GCCGGTGAGA GACTTCTACC AGGTACAGCG GGGTTACGCC 
ATCTGCCAAA CCACCCGCAT GGTCTCCTGG GTGGAGTGCA CCGGAGCTTG TGACACAGGG 
AGCTGCTGCA TCAGCCAGAG GATGAAGCGC CGGAAATACA CCTTTGAGTG CAGCGACGGA 
ACGTCCTTCA CCGAGGAGGT GGAAAAGACC ATCAAGTGCG GCTGCGTGGG CTGCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105938657 ( XM_012880472.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012735926.1 Fundulus heteroclitus slit homolog 1 (Drosophila) (slit1), transcript variant X2, mRNA.

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CloneID	OFu124013
Clone ID Related Accession (Same CDS sequence)	XM_012880472.1
Accession Version	XM_012880472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	slit homolog 1 protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234518.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA GAGCAAACAC CAACTCCACA AAGACGAGAC AAGAGAGGAG TTTTCAAGAA 
GGAAAGAAAG AAAGAAAGAA AGAAACCATC CCTTTTTTTT TTACATGGAC AAATTATAGT 
GGATATCAAA GCTGCATCAT CAGATCTGTA GGATTTGTGT GGAAGATCTT TTTTTTTTTT 
CTCCCTTCTA TTTATACTCT CCAGTTAAAT AAAATTGTTC CTGCAGGACT TGTGAGGTGT 
GGCAGGACTT TTTTGGAAGC CAAAAGGAAG GAGAACATAA AGAGAGGTGG ACAGAAACAA 
GTAAGGAGGG GAAAAAAAAA GAAGAAAAGG CTTAGGAACA TGCCCGCTGA AGACAGCAGT 
TTTGAATCAA GCATGGGCAG GCTGGCTGGG CAGTGGATGT GGCTGCTGAT GCTCTCTGCC 
TTGATGCTGG GCGATGCCAG CGCCTGCCCG GCGCTTTGTA CCTGCAGCGG CACCACAGTG 
GACTGCCACG GACTGGGGCT CAAAACTATG CCAAGGAGTA TCCCCCGCAA CACTGAGAGA 
CTGGAGCTGA ACGGAAACAA CCTCACACGC ATCACCAAGA GTGACTTTGC TGGCCTGAAG 
TACCTCAGAG TGCTACAGCT GATGGAGAAT CAGATTACAG TGATTGAGCG AGGAGCTTTC 
GATGACATGA AGGAGCTGGA GCGGCTGCGG CTGAACCGTA ATCAACTGCA GCAGCTTCCG 
GAGCTGCTGT TTCAGAAGAA CCCCGGCCTG TCTCGGCTCG ATCTGAGTGA GAACTTCATC 
CAATCCATCC CCAGAAAAGC CTTCAGAGGA GCGACAGACA TCAAAAACCT GCAGCTGGAT 
AAAAACCACA TCGGCTGCAT CGAGGATGGA GCCTTCCGAG CTCTAAGAGG CCTGGAAGTA 
CTAACGCTGA ACAACAACAA CATCAGCAGC ATCCCCGTCT CCAGCTTCAA CCACATGCCC 
AAACTCCGAA CTTTCCGTCT TCACTCCAAC AACTTAAACT GTGATTGTCA CCTGGCCTGG 
CTGTCCCAGT GGCTCAGACA GAGGCCTACG ATTGGTCTGT TCACCCAGTG TGCTTCGCCG 
CCAGAGCTAA GAGGACTCAA TGTGGCGGAG GTCCAGAAAC ACGAATTCTC CTGTTCAGGG 
CATCAGGAGT CTTCCAGCCT GCAGCCGTGC AGCATTGGCG GAGGCTCATG TCCCGCCATG 
TGCACCTGCA GTAACAACAT CGTGGACTGC AGAGGGAAAG GTCTCACTGC CATTCCAGCC 
AATCTGCCGG ACAGCATGTC AGAAATACGT CTGGAGCAGA ACGGGATTAA GTCTGTGCCT 
CCCGGAGCCT TCTCCCCCTA CAAGAAACTG CGCAGGATAG ACCTGAGCAA CAACCAGATC 
TCAGAGATAG CGCCTGACGC CTTCCAGGGA CTGCGATCTC TTAACTCTCT CGTGCTTTAC 
GGAAACAAGA TCACCGACCT GCCGAAGGGT GTGTTTGACG GCCTCTACTC TTTGCAACTG 
CTGCTGCTGA ATGCCAATAA GATTCACTGC GTCCGTGCCA ATGCCTTCCA GGATCTGCAG 
AATCTCTCCC TGCTATCGCT GTACGATAAC AAGATCCAAA CCCTCGCCAA GGGCACCTTC 
ACTTCCCTGC GAGCAATACA AACCCTGCAC CTGGCGCAGA ACCCGTTTAT TTGTGACTGT 
AATCTGAAGT GGCTGGCAGA CTACCTGCGC TCCAACCCAA TCGAGACCAG CGGCGCCCGC 
TGCGCCAGCC CACGGCGCCT CGCAAACAAA CGCATCGGAC AAATCAAGAG CAAGAAGTTC 
CGCTGCTCCG GTACAGAAGA CACCCGTCTC AATAACGCCT GCAACACTGA TCCCGTGTGT 
CCTCCCAAGT GTCGCTGTGA GTCTAACGTA GTGGACTGCT CCAACCTGAA ACTTACCAAG 
ATCCCCGAGC ACATCCCTGC ATCCACAGCT GAACTCCGCC TTAACAATAA CGAGATCACC 
AGCCTTGAGG CAACTGGAGT CTTTAAGAAC CTTTCCCATC TGAAGAAAAT TAACCTGAGT 
AACAACAAGA TCACAGAGAT CGAGGACGGG GCTTTTGAAG GTGCATCCTC TGTCATTGAG 
CTCCACCTCA CAGCCAATCA AATCGACTCG GTTCGCAGCG GGATGTTCCG AGGCCTCGAG 
GGACTGCGCA TGATGATGCT GCGGAACAAC AAAATCAGCT GTGTCCACAA CGACAGCTTC 
ACGGGGCTTC ACAACGTCCG TCTGCTGTCT CTGTACGACA ACCACCTGAC CACCATTACT 
CCTGGAGCAT TCGACACGCT GCAGACTCTC TCTACTCTAA ACCTCTTGGC CAACTCCTTC 
AACTGCGACT GCCGGCTGGC GTGGCTCGGC GACTGGCTGA GGAGCAGGAA GATTGTGACG 
GGTAACCCTC GCTGCCAGCG CCCGGCCTTC CTGAAGGAGA TCCCCCTGCA GGATGTAGCG 
CTGCCGGATT TTCGCTGCGA GGAAGGCCAA GAGGAGTCAA GCTGTGTTCC TCGACCCCAG 
TGTCCCAGCG AGTGTACTTG CCTAGAGACG GTGGTGCGCT GCAGCAACAA GCATCTCCAC 
ACACTACCCA AAGGCATCCC CAGGAATGTG ACTGAACTGT ACCTTGATGG CAACCAGTTC 
AGTGTTGTTC CCAAGGAACT GTCTTCCTTC AAATTCCTAC AGCTGGTTGA CCTGAGCAAC 
AACAAAATCA ACTCTCTGAC CAATTCATCC TTTGCTAACA TGAGCCAACT GACCACTCTA 
ATCCTGAGCT ACAACTCCCT GCGCTGCATA CCCAAAATGG CTTTCAGTGG CCTGCACTCA 
CTGCGACTGC TGTCACTTCA TGGTAATGAA ATCTCAGAGC TTCCAGATGG CATCTTCAAT 
GATGTGACCT CCCTCTCCCA TTTGGCGATC GGAGCCAACC CTCTGCACTG TGACTGCCAC 
CTGCGCTGGC TCTCCGACTG GGTGAAGACC GGATACAAAG AACCTGGGAT CGCGCGCTGT 
GCCGGCCCAC AGGGGATGGA GAGCAAGCTG CTCCTCACCA CGCCTGCCAA GAAATTTGAA 
TGCACAGGCG ATGTGGACAC GTCGGTGCTG GCCAAGTGTA ACCCCTGCCT GTCCAGTCCC 
TGCCTGAATC ACGGCATCTG TCACAATGAC CCATTGGAGA TCTACAGGTG CAGCTGTCCT 
CCTGGGTTCA AGGGCAAGAA CTGTGAGACG GCTGCGAATG CCTGTGTGAG TAGCCCATGC 
GCCAATGGAG GAACCTGCAA AGTGAATGAG GAGGACGAGG GAGAGTACCG CTGCTCCTGT 
CTGCCGGGCT TCGAGGGCCC GACTTGCCAA ATCAATGTTG ACGACTGTGA AGACAACGAC 
TGTGAGAACG GGGCGACCTG CATTGATGGA GTCAACAACT ACACTTGTTT TTGTCCCCCC 
TACTACACAG GGGAGATGTG TGAAGAAATG GACGATGTGT GTGCTCCAGG GCGAAGCCCC 
TGCCAACATC AGTCTACCTG CCTCATCAGC ACCTCTGGAC CCAAGTGTGT GTGTTCTCCT 
GGGTATGTTG GTGATGACTG CAGTGTCAAC TACGATGACT GCAGGGAGCA TCGCTGTCAG 
AACGGAGCAC AGTGCGTGGA TGAGCTGAAC GGATATTCCT GCATCTGCCC CGAAGGATAT 
AGCGGCCAGC TGTGTGAAGT TCCCCCATCT CCCCTGTCAC TGTGTGAGCT GGCCGACTGC 
CAAAACAACG CCCCCTGTGT GGAGCGAGGT GGCCGCGCCC TCTGCCAGTG TCCTCCAGAG 
TTCGGGGGTC CGCGCTGCGA AAAACTGGTC AGCGTCAACT TTGTCGACAG AGACTCCTAC 
TTATTGCTGT CCGACCTGGA GAACTGGCCA CAAGCTAACA TCACCCTGCA GGTTTCCACA 
GCTGAGGACA ACGGGATCCT GCTGTACAAT GGAGACAACG ATCACATTGC AGTGGAGCTC 
TTCCAAGGCC ACGTGAAAGT GAGCTACGAC CCGGGCAGCC AGCCAGGACA CGCCATATAC 
AGCACCGAGA CCATCAACGA TGGTCAGTTC CACACAGTGG AGCTGGTGAC CTTCGACCAG 
ATGGTGAACC TGTCCATTGA TGGGGGTCTC CCTACCACAA TGGACAGCTT TGGGGCTGTG 
CGGCCCCTCA ACGGAGAGGC GCCACTATAC GTGGGGGGTA GGGGCCCTCT CCAGAACACA 
CCTCCCTCCT CTGCAGGCAT GCCTGTGGAT GTCCACTCCG GCACGTTCCG TCTCTGGCAG 
ATCCAGAACG GCTCCAGCTT CCACGGCTGC ATCCAGAACC TGTACATCAA CAATGAGCTC 
CAGGACTTCA CCAAGACCCA AATGAAGCCC GGTGTGGTGC CAGGCTGCGA GCCCTGCAAG 
AAGCTCTACT GTGTCCATGG CATCTGCCAG CCTGACGGGT TACAGGGACC CGTGTGTCAC 
TGCCAGCCAG GCTGGAGCGG GGCGCACTGT GATCAGCCGG CCCTCAACCC CTGCCAGGGC 
AGCAAATGCG TCCATGGAAA GTGCATTCCT CTGGACTCTC AGTCTTACCG CTGCGAGTGC 
GAAGAGGGCT ACAGAGGCGC GCTTTGTAAC CAGCAAGGAG AGCTGTTCAA CCCCTGCCGC 
CGCCTCTCCT GCAAACACGG CCGCTGCCAG ATCTCCGACA CCGGAGACGC ATACTGTCAC 
TGCGAAAGCG GCTACACGGG GGAACTCTGT GATGCAGAGT CTGAATGTCG AGGCGAGCCG 
GTGAGAGACT TCTACCAGGT ACAGCGGGGT TACGCCATCT GCCAAACCAC CCGCATGGTC 
TCCTGGGTGG AGTGCACCGG AGCTTGTGAC ACAGGGAGCT GCTGCATCAG CCAGAGGATG 
AAGCGCCGGA AATACACCTT TGAGTGCAGC GACGGAACGT CCTTCACCGA GGAGGTGGAA 
AAGACCATCA AGTGCGGCTG CGTGGGCTGC ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012735926.1
CDS	109..5064
Translation	MQTRANTNSTKTRQERSFQEGKKERKKETIPFFFTWTNYSGYQSCIIRSVGFVWKIFFFFLPSIYTLQLNKIVPAGLVRCGRTFLEAKRKENIKRGGQKQVRRGKKKKKRLRNMPAEDSSFESSMGRLAGQWMWLLMLSALMLGDASACPALCTCSGTTVDCHGLGLKTMPRSIPRNTERLELNGNNLTRITKSDFAGLKYLRVLQLMENQITVIERGAFDDMKELERLRLNRNQLQQLPELLFQKNPGLSRLDLSENFIQSIPRKAFRGATDIKNLQLDKNHIGCIEDGAFRALRGLEVLTLNNNNISSIPVSSFNHMPKLRTFRLHSNNLNCDCHLAWLSQWLRQRPTIGLFTQCASPPELRGLNVAEVQKHEFSCSGHQESSSLQPCSIGGGSCPAMCTCSNNIVDCRGKGLTAIPANLPDSMSEIRLEQNGIKSVPPGAFSPYKKLRRIDLSNNQISEIAPDAFQGLRSLNSLVLYGNKITDLPKGVFDGLYSLQLLLLNANKIHCVRANAFQDLQNLSLLSLYDNKIQTLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADYLRSNPIETSGARCASPRRLANKRIGQIKSKKFRCSGTEDTRLNNACNTDPVCPPKCRCESNVVDCSNLKLTKIPEHIPASTAELRLNNNEITSLEATGVFKNLSHLKKINLSNNKITEIEDGAFEGASSVIELHLTANQIDSVRSGMFRGLEGLRMMMLRNNKISCVHNDSFTGLHNVRLLSLYDNHLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTGNPRCQRPAFLKEIPLQDVALPDFRCEEGQEESSCVPRPQCPSECTCLETVVRCSNKHLHTLPKGIPRNVTELYLDGNQFSVVPKELSSFKFLQLVDLSNNKINSLTNSSFANMSQLTTLILSYNSLRCIPKMAFSGLHSLRLLSLHGNEISELPDGIFNDVTSLSHLAIGANPLHCDCHLRWLSDWVKTGYKEPGIARCAGPQGMESKLLLTTPAKKFECTGDVDTSVLAKCNPCLSSPCLNHGICHNDPLEIYRCSCPPGFKGKNCETAANACVSSPCANGGTCKVNEEDEGEYRCSCLPGFEGPTCQINVDDCEDNDCENGATCIDGVNNYTCFCPPYYTGEMCEEMDDVCAPGRSPCQHQSTCLISTSGPKCVCSPGYVGDDCSVNYDDCREHRCQNGAQCVDELNGYSCICPEGYSGQLCEVPPSPLSLCELADCQNNAPCVERGGRALCQCPPEFGGPRCEKLVSVNFVDRDSYLLLSDLENWPQANITLQVSTAEDNGILLYNGDNDHIAVELFQGHVKVSYDPGSQPGHAIYSTETINDGQFHTVELVTFDQMVNLSIDGGLPTTMDSFGAVRPLNGEAPLYVGGRGPLQNTPPSSAGMPVDVHSGTFRLWQIQNGSSFHGCIQNLYINNELQDFTKTQMKPGVVPGCEPCKKLYCVHGICQPDGLQGPVCHCQPGWSGAHCDQPALNPCQGSKCVHGKCIPLDSQSYRCECEEGYRGALCNQQGELFNPCRRLSCKHGRCQISDTGDAYCHCESGYTGELCDAESECRGEPVRDFYQVQRGYAICQTTRMVSWVECTGACDTGSCCISQRMKRRKYTFECSDGTSFTEEVEKTIKCGCVGCM

Target ORF information:

RefSeq Version	XM_012880472.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus slit homolog 1 (Drosophila) (slit1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012880472.1

ORF Insert Sequence:

ATGCAGACCA GAGCAAACAC CAACTCCACA AAGACGAGAC AAGAGAGGAG TTTTCAAGAA 
GGAAAGAAAG AAAGAAAGAA AGAAACCATC CCTTTTTTTT TTACATGGAC AAATTATAGT 
GGATATCAAA GCTGCATCAT CAGATCTGTA GGATTTGTGT GGAAGATCTT TTTTTTTTTT 
CTCCCTTCTA TTTATACTCT CCAGTTAAAT AAAATTGTTC CTGCAGGACT TGTGAGGTGT 
GGCAGGACTT TTTTGGAAGC CAAAAGGAAG GAGAACATAA AGAGAGGTGG ACAGAAACAA 
GTAAGGAGGG GAAAAAAAAA GAAGAAAAGG CTTAGGAACA TGCCCGCTGA AGACAGCAGT 
TTTGAATCAA GCATGGGCAG GCTGGCTGGG CAGTGGATGT GGCTGCTGAT GCTCTCTGCC 
TTGATGCTGG GCGATGCCAG CGCCTGCCCG GCGCTTTGTA CCTGCAGCGG CACCACAGTG 
GACTGCCACG GACTGGGGCT CAAAACTATG CCAAGGAGTA TCCCCCGCAA CACTGAGAGA 
CTGGAGCTGA ACGGAAACAA CCTCACACGC ATCACCAAGA GTGACTTTGC TGGCCTGAAG 
TACCTCAGAG TGCTACAGCT GATGGAGAAT CAGATTACAG TGATTGAGCG AGGAGCTTTC 
GATGACATGA AGGAGCTGGA GCGGCTGCGG CTGAACCGTA ATCAACTGCA GCAGCTTCCG 
GAGCTGCTGT TTCAGAAGAA CCCCGGCCTG TCTCGGCTCG ATCTGAGTGA GAACTTCATC 
CAATCCATCC CCAGAAAAGC CTTCAGAGGA GCGACAGACA TCAAAAACCT GCAGCTGGAT 
AAAAACCACA TCGGCTGCAT CGAGGATGGA GCCTTCCGAG CTCTAAGAGG CCTGGAAGTA 
CTAACGCTGA ACAACAACAA CATCAGCAGC ATCCCCGTCT CCAGCTTCAA CCACATGCCC 
AAACTCCGAA CTTTCCGTCT TCACTCCAAC AACTTAAACT GTGATTGTCA CCTGGCCTGG 
CTGTCCCAGT GGCTCAGACA GAGGCCTACG ATTGGTCTGT TCACCCAGTG TGCTTCGCCG 
CCAGAGCTAA GAGGACTCAA TGTGGCGGAG GTCCAGAAAC ACGAATTCTC CTGTTCAGGG 
CATCAGGAGT CTTCCAGCCT GCAGCCGTGC AGCATTGGCG GAGGCTCATG TCCCGCCATG 
TGCACCTGCA GTAACAACAT CGTGGACTGC AGAGGGAAAG GTCTCACTGC CATTCCAGCC 
AATCTGCCGG ACAGCATGTC AGAAATACGT CTGGAGCAGA ACGGGATTAA GTCTGTGCCT 
CCCGGAGCCT TCTCCCCCTA CAAGAAACTG CGCAGGATAG ACCTGAGCAA CAACCAGATC 
TCAGAGATAG CGCCTGACGC CTTCCAGGGA CTGCGATCTC TTAACTCTCT CGTGCTTTAC 
GGAAACAAGA TCACCGACCT GCCGAAGGGT GTGTTTGACG GCCTCTACTC TTTGCAACTG 
CTGCTGCTGA ATGCCAATAA GATTCACTGC GTCCGTGCCA ATGCCTTCCA GGATCTGCAG 
AATCTCTCCC TGCTATCGCT GTACGATAAC AAGATCCAAA CCCTCGCCAA GGGCACCTTC 
ACTTCCCTGC GAGCAATACA AACCCTGCAC CTGGCGCAGA ACCCGTTTAT TTGTGACTGT 
AATCTGAAGT GGCTGGCAGA CTACCTGCGC TCCAACCCAA TCGAGACCAG CGGCGCCCGC 
TGCGCCAGCC CACGGCGCCT CGCAAACAAA CGCATCGGAC AAATCAAGAG CAAGAAGTTC 
CGCTGCTCCG GTACAGAAGA CACCCGTCTC AATAACGCCT GCAACACTGA TCCCGTGTGT 
CCTCCCAAGT GTCGCTGTGA GTCTAACGTA GTGGACTGCT CCAACCTGAA ACTTACCAAG 
ATCCCCGAGC ACATCCCTGC ATCCACAGCT GAACTCCGCC TTAACAATAA CGAGATCACC 
AGCCTTGAGG CAACTGGAGT CTTTAAGAAC CTTTCCCATC TGAAGAAAAT TAACCTGAGT 
AACAACAAGA TCACAGAGAT CGAGGACGGG GCTTTTGAAG GTGCATCCTC TGTCATTGAG 
CTCCACCTCA CAGCCAATCA AATCGACTCG GTTCGCAGCG GGATGTTCCG AGGCCTCGAG 
GGACTGCGCA TGATGATGCT GCGGAACAAC AAAATCAGCT GTGTCCACAA CGACAGCTTC 
ACGGGGCTTC ACAACGTCCG TCTGCTGTCT CTGTACGACA ACCACCTGAC CACCATTACT 
CCTGGAGCAT TCGACACGCT GCAGACTCTC TCTACTCTAA ACCTCTTGGC CAACTCCTTC 
AACTGCGACT GCCGGCTGGC GTGGCTCGGC GACTGGCTGA GGAGCAGGAA GATTGTGACG 
GGTAACCCTC GCTGCCAGCG CCCGGCCTTC CTGAAGGAGA TCCCCCTGCA GGATGTAGCG 
CTGCCGGATT TTCGCTGCGA GGAAGGCCAA GAGGAGTCAA GCTGTGTTCC TCGACCCCAG 
TGTCCCAGCG AGTGTACTTG CCTAGAGACG GTGGTGCGCT GCAGCAACAA GCATCTCCAC 
ACACTACCCA AAGGCATCCC CAGGAATGTG ACTGAACTGT ACCTTGATGG CAACCAGTTC 
AGTGTTGTTC CCAAGGAACT GTCTTCCTTC AAATTCCTAC AGCTGGTTGA CCTGAGCAAC 
AACAAAATCA ACTCTCTGAC CAATTCATCC TTTGCTAACA TGAGCCAACT GACCACTCTA 
ATCCTGAGCT ACAACTCCCT GCGCTGCATA CCCAAAATGG CTTTCAGTGG CCTGCACTCA 
CTGCGACTGC TGTCACTTCA TGGTAATGAA ATCTCAGAGC TTCCAGATGG CATCTTCAAT 
GATGTGACCT CCCTCTCCCA TTTGGCGATC GGAGCCAACC CTCTGCACTG TGACTGCCAC 
CTGCGCTGGC TCTCCGACTG GGTGAAGACC GGATACAAAG AACCTGGGAT CGCGCGCTGT 
GCCGGCCCAC AGGGGATGGA GAGCAAGCTG CTCCTCACCA CGCCTGCCAA GAAATTTGAA 
TGCACAGGCG ATGTGGACAC GTCGGTGCTG GCCAAGTGTA ACCCCTGCCT GTCCAGTCCC 
TGCCTGAATC ACGGCATCTG TCACAATGAC CCATTGGAGA TCTACAGGTG CAGCTGTCCT 
CCTGGGTTCA AGGGCAAGAA CTGTGAGACG GCTGCGAATG CCTGTGTGAG TAGCCCATGC 
GCCAATGGAG GAACCTGCAA AGTGAATGAG GAGGACGAGG GAGAGTACCG CTGCTCCTGT 
CTGCCGGGCT TCGAGGGCCC GACTTGCCAA ATCAATGTTG ACGACTGTGA AGACAACGAC 
TGTGAGAACG GGGCGACCTG CATTGATGGA GTCAACAACT ACACTTGTTT TTGTCCCCCC 
TACTACACAG GGGAGATGTG TGAAGAAATG GACGATGTGT GTGCTCCAGG GCGAAGCCCC 
TGCCAACATC AGTCTACCTG CCTCATCAGC ACCTCTGGAC CCAAGTGTGT GTGTTCTCCT 
GGGTATGTTG GTGATGACTG CAGTGTCAAC TACGATGACT GCAGGGAGCA TCGCTGTCAG 
AACGGAGCAC AGTGCGTGGA TGAGCTGAAC GGATATTCCT GCATCTGCCC CGAAGGATAT 
AGCGGCCAGC TGTGTGAAGT TCCCCCATCT CCCCTGTCAC TGTGTGAGCT GGCCGACTGC 
CAAAACAACG CCCCCTGTGT GGAGCGAGGT GGCCGCGCCC TCTGCCAGTG TCCTCCAGAG 
TTCGGGGGTC CGCGCTGCGA AAAACTGGTC AGCGTCAACT TTGTCGACAG AGACTCCTAC 
TTATTGCTGT CCGACCTGGA GAACTGGCCA CAAGCTAACA TCACCCTGCA GGTTTCCACA 
GCTGAGGACA ACGGGATCCT GCTGTACAAT GGAGACAACG ATCACATTGC AGTGGAGCTC 
TTCCAAGGCC ACGTGAAAGT GAGCTACGAC CCGGGCAGCC AGCCAGGACA CGCCATATAC 
AGCACCGAGA CCATCAACGA TGGTCAGTTC CACACAGTGG AGCTGGTGAC CTTCGACCAG 
ATGGTGAACC TGTCCATTGA TGGGGGTCTC CCTACCACAA TGGACAGCTT TGGGGCTGTG 
CGGCCCCTCA ACGGAGAGGC GCCACTATAC GTGGGGGGTA GGGGCCCTCT CCAGAACACA 
CCTCCCTCCT CTGCAGGCAT GCCTGTGGAT GTCCACTCCG GCACGTTCCG TCTCTGGCAG 
ATCCAGAACG GCTCCAGCTT CCACGGCTGC ATCCAGAACC TGTACATCAA CAATGAGCTC 
CAGGACTTCA CCAAGACCCA AATGAAGCCC GGTGTGGTGC CAGGCTGCGA GCCCTGCAAG 
AAGCTCTACT GTGTCCATGG CATCTGCCAG CCTGACGGGT TACAGGGACC CGTGTGTCAC 
TGCCAGCCAG GCTGGAGCGG GGCGCACTGT GATCAGCCGG CCCTCAACCC CTGCCAGGGC 
AGCAAATGCG TCCATGGAAA GTGCATTCCT CTGGACTCTC AGTCTTACCG CTGCGAGTGC 
GAAGAGGGCT ACAGAGGCGC GCTTTGTAAC CAGCAAGGAG AGCTGTTCAA CCCCTGCCGC 
CGCCTCTCCT GCAAACACGG CCGCTGCCAG ATCTCCGACA CCGGAGACGC ATACTGTCAC 
TGCGAAAGCG GCTACACGGG GGAACTCTGT GATGCAGAGT CTGAATGTCG AGGCGAGCCG 
GTGAGAGACT TCTACCAGGT ACAGCGGGGT TACGCCATCT GCCAAACCAC CCGCATGGTC 
TCCTGGGTGG AGTGCACCGG AGCTTGTGAC ACAGGGAGCT GCTGCATCAG CCAGAGGATG 
AAGCGCCGGA AATACACCTT TGAGTGCAGC GACGGAACGT CCTTCACCGA GGAGGTGGAA 
AAGACCATCA AGTGCGGCTG CGTGGGCTGC ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105938657 ( XM_012880473.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012735927.1 Fundulus heteroclitus slit homolog 1 (Drosophila) (slit1), transcript variant X3, mRNA.

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CloneID	OFu124014
Clone ID Related Accession (Same CDS sequence)	XM_012880473.1 , XM_012880473.2
Accession Version	XM_012880473.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	slit homolog 1 protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234518.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA GAGCAAACAC CAACTCCACA AAGACGAGAC AAGAGAGGAG TTTTCAAGAA 
GGAAAGAAAG AAAGAAAGAA AGAAACCATC CCTTTTTTTT TTACATGGAC AAATTATAGT 
GGATATCAAA GCTGCATCAT CAGATCTGTA GGATTTGTGT GGAAGATCTT TTTTTTTTTT 
CTCCCTTCTA TTTATACTCT CCAGTTAAAT AAAATTGTTC CTGCAGGACT TGTGAGGTGT 
GGCAGGACTT TTTTGGAAGC CAAAAGGAAG GAGAACATAA AGAGAGGTGG ACAGAAACAA 
GTAAGGAGGG GAAAAAAAAA GAAGAAAAGG CTTAGGAACA TGCCCGCTGA AGACAGCAGT 
TTTGAATCAA GCATGGGCAG GCTGGCTGGG CAGTGGATGT GGCTGCTGAT GCTCTCTGCC 
TTGATGCTGG GCGATGCCAG CGCCTGCCCG GCGCTTTGTA CCTGCAGCGG CACCACAGTG 
GACTGCCACG GACTGGGGCT CAAAACTATG CCAAGGAGTA TCCCCCGCAA CACTGAGAGA 
CTGGAGCTGA ACGGAAACAA CCTCACACGC ATCACCAAGA GTGACTTTGC TGGCCTGAAG 
TACCTCAGAG TGCTACAGCT GATGGAGAAT CAGATTACAG TGATTGAGCG AGGAGCTTTC 
GATGACATGA AGGAGCTGGA GCGGCTGCGG CTGAACCGTA ATCAACTGCA GCAGCTTCCG 
GAGCTGCTGT TTCAGAAGAA CCCCGGCCTG TCTCGGCTCG ATCTGAGTGA GAACTTCATC 
CAATCCATCC CCAGAAAAGC CTTCAGAGGA GCGACAGACA TCAAAAACCT GCAGCTGGAT 
AAAAACCACA TCGGCTGCAT CGAGGATGGA GCCTTCCGAG CTCTAAGAGG CCTGGAAGTA 
CTAACGCTGA ACAACAACAA CATCAGCAGC ATCCCCGTCT CCAGCTTCAA CCACATGCCC 
AAACTCCGAA CTTTCCGTCT TCACTCCAAC AACTTAAACT GTGATTGTCA CCTGGCCTGG 
CTGTCCCAGT GGCTCAGACA GAGGCCTACG ATTGGTCTGT TCACCCAGTG TGCTTCGCCG 
CCAGAGCTAA GAGGACTCAA TGTGGCGGAG GTCCAGAAAC ACGAATTCTC CTGTTCAGGG 
CATCAGGAGT CTTCCAGCCT GCAGCCGTGC AGCATTGGCG GAGGCTCATG TCCCGCCATG 
TGCACCTGCA GTAACAACAT CGTGGACTGC AGAGGGAAAG GTCTCACTGC CATTCCAGCC 
AATCTGCCGG ACAGCATGTC AGAAATACGT CTGGAGCAGA ACGGGATTAA GTCTGTGCCT 
CCCGGAGCCT TCTCCCCCTA CAAGAAACTG CGCAGGATAG ACCTGAGCAA CAACCAGATC 
TCAGAGATAG CGCCTGACGC CTTCCAGGGA CTGCGATCTC TTAACTCTCT CGTGCTTTAC 
GGAAACAAGA TCACCGACCT GCCGAAGGGT GTGTTTGACG GCCTCTACTC TTTGCAACTG 
CTGCTGCTGA ATGCCAATAA GATTCACTGC GTCCGTGCCA ATGCCTTCCA GGATCTGCAG 
AATCTCTCCC TGCTATCGCT GTACGATAAC AAGATCCAAA CCCTCGCCAA GGGCACCTTC 
ACTTCCCTGC GAGCAATACA AACCCTGCAC CTGGCGCAGA ACCCGTTTAT TTGTGACTGT 
AATCTGAAGT GGCTGGCAGA CTACCTGCGC TCCAACCCAA TCGAGACCAG CGGCGCCCGC 
TGCGCCAGCC CACGGCGCCT CGCAAACAAA CGCATCGGAC AAATCAAGAG CAAGAAGTTC 
CGCTGCTCCG CCAAAGAGCA GTACGTCATC CCAGGTACAG AAGACACCCG TCTCAATAAC 
GCCTGCAACA CTGATCCCGT GTGTCCTCCC AAGTGTCGCT GTGAGTCTAA CGTAGTGGAC 
TGCTCCAACC TGAAACTTAC CAAGATCCCC GAGCACATCC CTGCATCCAC AGCTGAACTC 
CGCCTTAACA ATAACGAGAT CACCAGCCTT GAGGCAACTG GAGTCTTTAA GAACCTTTCC 
CATCTGAAGA AAATTAACCT GAGTAACAAC AAGATCACAG AGATCGAGGA CGGGGCTTTT 
GAAGGTGCAT CCTCTGTCAT TGAGCTCCAC CTCACAGCCA ATCAAATCGA CTCGGTTCGC 
AGCGGGATGT TCCGAGGCCT CGAGGGACTG CGCATGATGA TGCTGCGGAA CAACAAAATC 
AGCTGTGTCC ACAACGACAG CTTCACGGGG CTTCACAACG TCCGTCTGCT GTCTCTGTAC 
GACAACCACC TGACCACCAT TACTCCTGGA GCATTCGACA CGCTGCAGAC TCTCTCTACT 
CTAAACCTCT TGGCCAACTC CTTCAACTGC GACTGCCGGC TGGCGTGGCT CGGCGACTGG 
CTGAGGAGCA GGAAGATTGT GACGGGTAAC CCTCGCTGCC AGCGCCCGGC CTTCCTGAAG 
GAGATCCCCC TGCAGGATGT AGCGCTGCCG GATTTTCGCT GCGAGGAAGG CCAAGAGGAG 
TCAAGCTGTG TTCCTCGACC CCAGTGTCCC AGCGAGTGTA CTTGCCTAGA GACGGTGGTG 
CGCTGCAGCA ACAAGCATCT CCACACACTA CCCAAAGGCA TCCCCAGGAA TGTGACTGAA 
CTGTACCTTG ATGGCAACCA GTTCAGTGTT GTTCCCAAGG AACTGTCTTC CTTCAAATTC 
CTACAGCTGG TTGACCTGAG CAACAACAAA ATCAACTCTC TGACCAATTC ATCCTTTGCT 
AACATGAGCC AACTGACCAC TCTAATCCTG AGCTACAACT CCCTGCGCTG CATACCCAAA 
ATGGCTTTCA GTGGCCTGCA CTCACTGCGA CTGCTGTCAC TTCATGGTAA TGAAATCTCA 
GAGCTTCCAG ATGGCATCTT CAATGATGTG ACCTCCCTCT CCCATTTGGC GATCGGAGCC 
AACCCTCTGC ACTGTGACTG CCACCTGCGC TGGCTCTCCG ACTGGGTGAA GACCGGATAC 
AAAGAACCTG GGATCGCGCG CTGTGCCGGC CCACAGGGGA TGGAGAGCAA GCTGCTCCTC 
ACCACGCCTG CCAAGAAATT TGAATGCACA GGCGATGTGG ACACGTCGGT GCTGGCCAAG 
TGTAACCCCT GCCTGTCCAG TCCCTGCCTG AATCACGGCA TCTGTCACAA TGACCCATTG 
GAGATCTACA GGTGCAGCTG TCCTCCTGGG TTCAAGGGCA AGAACTGTGA GACGGCTGCG 
AATGCCTGTG TGAGTAGCCC ATGCGCCAAT GGAGGAACCT GCAAAGTGAA TGAGGAGGAC 
GAGGGAGAGT ACCGCTGCTC CTGTCTGCCG GGCTTCGAGG GCCCGACTTG CCAAATCAAT 
GTTGACGACT GTGAAGACAA CGACTGTGAG AACGGGGCGA CCTGCATTGA TGGAGTCAAC 
AACTACACTT GTTTTTGTCC CCCCTACTAC ACAGGGGAGA TGTGTGAAGA AATGGACGAT 
GTGTGTGCTC CAGGGCGAAG CCCCTGCCAA CATCAGTCTA CCTGCCTCAT CAGCACCTCT 
GGACCCAAGT GTGTGTGTTC TCCTGGGTAT GTTGGTGATG ACTGCAGTGT CAACTACGAT 
GACTGCAGGG AGCATCGCTG TCAGAACGGA GCACAGTGCG TGGATGAGCT GAACGGATAT 
TCCTGCATCT GCCCCGAAGG ATATAGCGGC CAGCTGTGTG AAGTTCCCCC ATCTCCCCTG 
TCACTGTGTG AGCTGGCCGA CTGCCAAAAC AACGCCCCCT GTGTGGAGCG AGGTGGCCGC 
GCCCTCTGCC AGTGTCCTCC AGAGTTCGGG GGTCCGCGCT GCGAAAAACT GGTCAGCGTC 
AACTTTGTCG ACAGAGACTC CTACTTATTG CTGTCCGACC TGGAGAACTG GCCACAAGCT 
AACATCACCC TGCAGGTTTC CACAGCTGAG GACAACGGGA TCCTGCTGTA CAATGGAGAC 
AACGATCACA TTGCAGTGGA GCTCTTCCAA GGCCACGTGA AAGTGAGCTA CGACCCGGGC 
AGCCAGCCAG GACACGCCAT ATACAGCACC GAGACCATCA ACGATGGTCA GTTCCACACA 
GTGGAGCTGG TGACCTTCGA CCAGATGGTG AACCTGTCCA TTGATGGGGG TCTCCCTACC 
ACAATGGACA GCTTTGGGGC TGTGCGGCCC CTCAACGGAG AGGCGCCACT ATACGTGGGG 
GGCATGCCTG TGGATGTCCA CTCCGGCACG TTCCGTCTCT GGCAGATCCA GAACGGCTCC 
AGCTTCCACG GCTGCATCCA GAACCTGTAC ATCAACAATG AGCTCCAGGA CTTCACCAAG 
ACCCAAATGA AGCCCGGTGT GGTGCCAGGC TGCGAGCCCT GCAAGAAGCT CTACTGTGTC 
CATGGCATCT GCCAGCCTGA CGGGTTACAG GGACCCGTGT GTCACTGCCA GCCAGGCTGG 
AGCGGGGCGC ACTGTGATCA GCCGGCCCTC AACCCCTGCC AGGGCAGCAA ATGCGTCCAT 
GGAAAGTGCA TTCCTCTGGA CTCTCAGTCT TACCGCTGCG AGTGCGAAGA GGGCTACAGA 
GGCGCGCTTT GTAACCAGCA AGGAGAGCTG TTCAACCCCT GCCGCCGCCT CTCCTGCAAA 
CACGGCCGCT GCCAGATCTC CGACACCGGA GACGCATACT GTCACTGCGA AAGCGGCTAC 
ACGGGGGAAC TCTGTGATGC AGAGTCTGAA TGTCGAGGCG AGCCGGTGAG AGACTTCTAC 
CAGGTACAGC GGGGTTACGC CATCTGCCAA ACCACCCGCA TGGTCTCCTG GGTGGAGTGC 
ACCGGAGCTT GTGACACAGG GAGCTGCTGC ATCAGCCAGA GGATGAAGCG CCGGAAATAC 
ACCTTTGAGT GCAGCGACGG AACGTCCTTC ACCGAGGAGG TGGAAAAGAC CATCAAGTGC 
GGCTGCGTGG GCTGCATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012735927.1
CDS	108..5048
Translation	MQTRANTNSTKTRQERSFQEGKKERKKETIPFFFTWTNYSGYQSCIIRSVGFVWKIFFFFLPSIYTLQLNKIVPAGLVRCGRTFLEAKRKENIKRGGQKQVRRGKKKKKRLRNMPAEDSSFESSMGRLAGQWMWLLMLSALMLGDASACPALCTCSGTTVDCHGLGLKTMPRSIPRNTERLELNGNNLTRITKSDFAGLKYLRVLQLMENQITVIERGAFDDMKELERLRLNRNQLQQLPELLFQKNPGLSRLDLSENFIQSIPRKAFRGATDIKNLQLDKNHIGCIEDGAFRALRGLEVLTLNNNNISSIPVSSFNHMPKLRTFRLHSNNLNCDCHLAWLSQWLRQRPTIGLFTQCASPPELRGLNVAEVQKHEFSCSGHQESSSLQPCSIGGGSCPAMCTCSNNIVDCRGKGLTAIPANLPDSMSEIRLEQNGIKSVPPGAFSPYKKLRRIDLSNNQISEIAPDAFQGLRSLNSLVLYGNKITDLPKGVFDGLYSLQLLLLNANKIHCVRANAFQDLQNLSLLSLYDNKIQTLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADYLRSNPIETSGARCASPRRLANKRIGQIKSKKFRCSAKEQYVIPGTEDTRLNNACNTDPVCPPKCRCESNVVDCSNLKLTKIPEHIPASTAELRLNNNEITSLEATGVFKNLSHLKKINLSNNKITEIEDGAFEGASSVIELHLTANQIDSVRSGMFRGLEGLRMMMLRNNKISCVHNDSFTGLHNVRLLSLYDNHLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTGNPRCQRPAFLKEIPLQDVALPDFRCEEGQEESSCVPRPQCPSECTCLETVVRCSNKHLHTLPKGIPRNVTELYLDGNQFSVVPKELSSFKFLQLVDLSNNKINSLTNSSFANMSQLTTLILSYNSLRCIPKMAFSGLHSLRLLSLHGNEISELPDGIFNDVTSLSHLAIGANPLHCDCHLRWLSDWVKTGYKEPGIARCAGPQGMESKLLLTTPAKKFECTGDVDTSVLAKCNPCLSSPCLNHGICHNDPLEIYRCSCPPGFKGKNCETAANACVSSPCANGGTCKVNEEDEGEYRCSCLPGFEGPTCQINVDDCEDNDCENGATCIDGVNNYTCFCPPYYTGEMCEEMDDVCAPGRSPCQHQSTCLISTSGPKCVCSPGYVGDDCSVNYDDCREHRCQNGAQCVDELNGYSCICPEGYSGQLCEVPPSPLSLCELADCQNNAPCVERGGRALCQCPPEFGGPRCEKLVSVNFVDRDSYLLLSDLENWPQANITLQVSTAEDNGILLYNGDNDHIAVELFQGHVKVSYDPGSQPGHAIYSTETINDGQFHTVELVTFDQMVNLSIDGGLPTTMDSFGAVRPLNGEAPLYVGGMPVDVHSGTFRLWQIQNGSSFHGCIQNLYINNELQDFTKTQMKPGVVPGCEPCKKLYCVHGICQPDGLQGPVCHCQPGWSGAHCDQPALNPCQGSKCVHGKCIPLDSQSYRCECEEGYRGALCNQQGELFNPCRRLSCKHGRCQISDTGDAYCHCESGYTGELCDAESECRGEPVRDFYQVQRGYAICQTTRMVSWVECTGACDTGSCCISQRMKRRKYTFECSDGTSFTEEVEKTIKCGCVGCM

Target ORF information:

RefSeq Version	XM_012880473.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus slit homolog 1 (Drosophila) (slit1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012880473.1

ORF Insert Sequence:

ATGCAGACCA GAGCAAACAC CAACTCCACA AAGACGAGAC AAGAGAGGAG TTTTCAAGAA 
GGAAAGAAAG AAAGAAAGAA AGAAACCATC CCTTTTTTTT TTACATGGAC AAATTATAGT 
GGATATCAAA GCTGCATCAT CAGATCTGTA GGATTTGTGT GGAAGATCTT TTTTTTTTTT 
CTCCCTTCTA TTTATACTCT CCAGTTAAAT AAAATTGTTC CTGCAGGACT TGTGAGGTGT 
GGCAGGACTT TTTTGGAAGC CAAAAGGAAG GAGAACATAA AGAGAGGTGG ACAGAAACAA 
GTAAGGAGGG GAAAAAAAAA GAAGAAAAGG CTTAGGAACA TGCCCGCTGA AGACAGCAGT 
TTTGAATCAA GCATGGGCAG GCTGGCTGGG CAGTGGATGT GGCTGCTGAT GCTCTCTGCC 
TTGATGCTGG GCGATGCCAG CGCCTGCCCG GCGCTTTGTA CCTGCAGCGG CACCACAGTG 
GACTGCCACG GACTGGGGCT CAAAACTATG CCAAGGAGTA TCCCCCGCAA CACTGAGAGA 
CTGGAGCTGA ACGGAAACAA CCTCACACGC ATCACCAAGA GTGACTTTGC TGGCCTGAAG 
TACCTCAGAG TGCTACAGCT GATGGAGAAT CAGATTACAG TGATTGAGCG AGGAGCTTTC 
GATGACATGA AGGAGCTGGA GCGGCTGCGG CTGAACCGTA ATCAACTGCA GCAGCTTCCG 
GAGCTGCTGT TTCAGAAGAA CCCCGGCCTG TCTCGGCTCG ATCTGAGTGA GAACTTCATC 
CAATCCATCC CCAGAAAAGC CTTCAGAGGA GCGACAGACA TCAAAAACCT GCAGCTGGAT 
AAAAACCACA TCGGCTGCAT CGAGGATGGA GCCTTCCGAG CTCTAAGAGG CCTGGAAGTA 
CTAACGCTGA ACAACAACAA CATCAGCAGC ATCCCCGTCT CCAGCTTCAA CCACATGCCC 
AAACTCCGAA CTTTCCGTCT TCACTCCAAC AACTTAAACT GTGATTGTCA CCTGGCCTGG 
CTGTCCCAGT GGCTCAGACA GAGGCCTACG ATTGGTCTGT TCACCCAGTG TGCTTCGCCG 
CCAGAGCTAA GAGGACTCAA TGTGGCGGAG GTCCAGAAAC ACGAATTCTC CTGTTCAGGG 
CATCAGGAGT CTTCCAGCCT GCAGCCGTGC AGCATTGGCG GAGGCTCATG TCCCGCCATG 
TGCACCTGCA GTAACAACAT CGTGGACTGC AGAGGGAAAG GTCTCACTGC CATTCCAGCC 
AATCTGCCGG ACAGCATGTC AGAAATACGT CTGGAGCAGA ACGGGATTAA GTCTGTGCCT 
CCCGGAGCCT TCTCCCCCTA CAAGAAACTG CGCAGGATAG ACCTGAGCAA CAACCAGATC 
TCAGAGATAG CGCCTGACGC CTTCCAGGGA CTGCGATCTC TTAACTCTCT CGTGCTTTAC 
GGAAACAAGA TCACCGACCT GCCGAAGGGT GTGTTTGACG GCCTCTACTC TTTGCAACTG 
CTGCTGCTGA ATGCCAATAA GATTCACTGC GTCCGTGCCA ATGCCTTCCA GGATCTGCAG 
AATCTCTCCC TGCTATCGCT GTACGATAAC AAGATCCAAA CCCTCGCCAA GGGCACCTTC 
ACTTCCCTGC GAGCAATACA AACCCTGCAC CTGGCGCAGA ACCCGTTTAT TTGTGACTGT 
AATCTGAAGT GGCTGGCAGA CTACCTGCGC TCCAACCCAA TCGAGACCAG CGGCGCCCGC 
TGCGCCAGCC CACGGCGCCT CGCAAACAAA CGCATCGGAC AAATCAAGAG CAAGAAGTTC 
CGCTGCTCCG CCAAAGAGCA GTACGTCATC CCAGGTACAG AAGACACCCG TCTCAATAAC 
GCCTGCAACA CTGATCCCGT GTGTCCTCCC AAGTGTCGCT GTGAGTCTAA CGTAGTGGAC 
TGCTCCAACC TGAAACTTAC CAAGATCCCC GAGCACATCC CTGCATCCAC AGCTGAACTC 
CGCCTTAACA ATAACGAGAT CACCAGCCTT GAGGCAACTG GAGTCTTTAA GAACCTTTCC 
CATCTGAAGA AAATTAACCT GAGTAACAAC AAGATCACAG AGATCGAGGA CGGGGCTTTT 
GAAGGTGCAT CCTCTGTCAT TGAGCTCCAC CTCACAGCCA ATCAAATCGA CTCGGTTCGC 
AGCGGGATGT TCCGAGGCCT CGAGGGACTG CGCATGATGA TGCTGCGGAA CAACAAAATC 
AGCTGTGTCC ACAACGACAG CTTCACGGGG CTTCACAACG TCCGTCTGCT GTCTCTGTAC 
GACAACCACC TGACCACCAT TACTCCTGGA GCATTCGACA CGCTGCAGAC TCTCTCTACT 
CTAAACCTCT TGGCCAACTC CTTCAACTGC GACTGCCGGC TGGCGTGGCT CGGCGACTGG 
CTGAGGAGCA GGAAGATTGT GACGGGTAAC CCTCGCTGCC AGCGCCCGGC CTTCCTGAAG 
GAGATCCCCC TGCAGGATGT AGCGCTGCCG GATTTTCGCT GCGAGGAAGG CCAAGAGGAG 
TCAAGCTGTG TTCCTCGACC CCAGTGTCCC AGCGAGTGTA CTTGCCTAGA GACGGTGGTG 
CGCTGCAGCA ACAAGCATCT CCACACACTA CCCAAAGGCA TCCCCAGGAA TGTGACTGAA 
CTGTACCTTG ATGGCAACCA GTTCAGTGTT GTTCCCAAGG AACTGTCTTC CTTCAAATTC 
CTACAGCTGG TTGACCTGAG CAACAACAAA ATCAACTCTC TGACCAATTC ATCCTTTGCT 
AACATGAGCC AACTGACCAC TCTAATCCTG AGCTACAACT CCCTGCGCTG CATACCCAAA 
ATGGCTTTCA GTGGCCTGCA CTCACTGCGA CTGCTGTCAC TTCATGGTAA TGAAATCTCA 
GAGCTTCCAG ATGGCATCTT CAATGATGTG ACCTCCCTCT CCCATTTGGC GATCGGAGCC 
AACCCTCTGC ACTGTGACTG CCACCTGCGC TGGCTCTCCG ACTGGGTGAA GACCGGATAC 
AAAGAACCTG GGATCGCGCG CTGTGCCGGC CCACAGGGGA TGGAGAGCAA GCTGCTCCTC 
ACCACGCCTG CCAAGAAATT TGAATGCACA GGCGATGTGG ACACGTCGGT GCTGGCCAAG 
TGTAACCCCT GCCTGTCCAG TCCCTGCCTG AATCACGGCA TCTGTCACAA TGACCCATTG 
GAGATCTACA GGTGCAGCTG TCCTCCTGGG TTCAAGGGCA AGAACTGTGA GACGGCTGCG 
AATGCCTGTG TGAGTAGCCC ATGCGCCAAT GGAGGAACCT GCAAAGTGAA TGAGGAGGAC 
GAGGGAGAGT ACCGCTGCTC CTGTCTGCCG GGCTTCGAGG GCCCGACTTG CCAAATCAAT 
GTTGACGACT GTGAAGACAA CGACTGTGAG AACGGGGCGA CCTGCATTGA TGGAGTCAAC 
AACTACACTT GTTTTTGTCC CCCCTACTAC ACAGGGGAGA TGTGTGAAGA AATGGACGAT 
GTGTGTGCTC CAGGGCGAAG CCCCTGCCAA CATCAGTCTA CCTGCCTCAT CAGCACCTCT 
GGACCCAAGT GTGTGTGTTC TCCTGGGTAT GTTGGTGATG ACTGCAGTGT CAACTACGAT 
GACTGCAGGG AGCATCGCTG TCAGAACGGA GCACAGTGCG TGGATGAGCT GAACGGATAT 
TCCTGCATCT GCCCCGAAGG ATATAGCGGC CAGCTGTGTG AAGTTCCCCC ATCTCCCCTG 
TCACTGTGTG AGCTGGCCGA CTGCCAAAAC AACGCCCCCT GTGTGGAGCG AGGTGGCCGC 
GCCCTCTGCC AGTGTCCTCC AGAGTTCGGG GGTCCGCGCT GCGAAAAACT GGTCAGCGTC 
AACTTTGTCG ACAGAGACTC CTACTTATTG CTGTCCGACC TGGAGAACTG GCCACAAGCT 
AACATCACCC TGCAGGTTTC CACAGCTGAG GACAACGGGA TCCTGCTGTA CAATGGAGAC 
AACGATCACA TTGCAGTGGA GCTCTTCCAA GGCCACGTGA AAGTGAGCTA CGACCCGGGC 
AGCCAGCCAG GACACGCCAT ATACAGCACC GAGACCATCA ACGATGGTCA GTTCCACACA 
GTGGAGCTGG TGACCTTCGA CCAGATGGTG AACCTGTCCA TTGATGGGGG TCTCCCTACC 
ACAATGGACA GCTTTGGGGC TGTGCGGCCC CTCAACGGAG AGGCGCCACT ATACGTGGGG 
GGCATGCCTG TGGATGTCCA CTCCGGCACG TTCCGTCTCT GGCAGATCCA GAACGGCTCC 
AGCTTCCACG GCTGCATCCA GAACCTGTAC ATCAACAATG AGCTCCAGGA CTTCACCAAG 
ACCCAAATGA AGCCCGGTGT GGTGCCAGGC TGCGAGCCCT GCAAGAAGCT CTACTGTGTC 
CATGGCATCT GCCAGCCTGA CGGGTTACAG GGACCCGTGT GTCACTGCCA GCCAGGCTGG 
AGCGGGGCGC ACTGTGATCA GCCGGCCCTC AACCCCTGCC AGGGCAGCAA ATGCGTCCAT 
GGAAAGTGCA TTCCTCTGGA CTCTCAGTCT TACCGCTGCG AGTGCGAAGA GGGCTACAGA 
GGCGCGCTTT GTAACCAGCA AGGAGAGCTG TTCAACCCCT GCCGCCGCCT CTCCTGCAAA 
CACGGCCGCT GCCAGATCTC CGACACCGGA GACGCATACT GTCACTGCGA AAGCGGCTAC 
ACGGGGGAAC TCTGTGATGC AGAGTCTGAA TGTCGAGGCG AGCCGGTGAG AGACTTCTAC 
CAGGTACAGC GGGGTTACGC CATCTGCCAA ACCACCCGCA TGGTCTCCTG GGTGGAGTGC 
ACCGGAGCTT GTGACACAGG GAGCTGCTGC ATCAGCCAGA GGATGAAGCG CCGGAAATAC 
ACCTTTGAGT GCAGCGACGG AACGTCCTTC ACCGAGGAGG TGGAAAAGAC CATCAAGTGC 
GGCTGCGTGG GCTGCATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105938657 ( XM_012880473.2 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012735927.1 Fundulus heteroclitus slit homolog 1 protein (LOC105938657), transcript variant X2, mRNA.

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CloneID	OFu124014
Clone ID Related Accession (Same CDS sequence)	XM_012880473.1 , XM_012880473.2
Accession Version	XM_012880473.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	slit homolog 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234518.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 1, 2017 this sequence version replaced XM_012880473.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA GAGCAAACAC CAACTCCACA AAGACGAGAC AAGAGAGGAG TTTTCAAGAA 
GGAAAGAAAG AAAGAAAGAA AGAAACCATC CCTTTTTTTT TTACATGGAC AAATTATAGT 
GGATATCAAA GCTGCATCAT CAGATCTGTA GGATTTGTGT GGAAGATCTT TTTTTTTTTT 
CTCCCTTCTA TTTATACTCT CCAGTTAAAT AAAATTGTTC CTGCAGGACT TGTGAGGTGT 
GGCAGGACTT TTTTGGAAGC CAAAAGGAAG GAGAACATAA AGAGAGGTGG ACAGAAACAA 
GTAAGGAGGG GAAAAAAAAA GAAGAAAAGG CTTAGGAACA TGCCCGCTGA AGACAGCAGT 
TTTGAATCAA GCATGGGCAG GCTGGCTGGG CAGTGGATGT GGCTGCTGAT GCTCTCTGCC 
TTGATGCTGG GCGATGCCAG CGCCTGCCCG GCGCTTTGTA CCTGCAGCGG CACCACAGTG 
GACTGCCACG GACTGGGGCT CAAAACTATG CCAAGGAGTA TCCCCCGCAA CACTGAGAGA 
CTGGAGCTGA ACGGAAACAA CCTCACACGC ATCACCAAGA GTGACTTTGC TGGCCTGAAG 
TACCTCAGAG TGCTACAGCT GATGGAGAAT CAGATTACAG TGATTGAGCG AGGAGCTTTC 
GATGACATGA AGGAGCTGGA GCGGCTGCGG CTGAACCGTA ATCAACTGCA GCAGCTTCCG 
GAGCTGCTGT TTCAGAAGAA CCCCGGCCTG TCTCGGCTCG ATCTGAGTGA GAACTTCATC 
CAATCCATCC CCAGAAAAGC CTTCAGAGGA GCGACAGACA TCAAAAACCT GCAGCTGGAT 
AAAAACCACA TCGGCTGCAT CGAGGATGGA GCCTTCCGAG CTCTAAGAGG CCTGGAAGTA 
CTAACGCTGA ACAACAACAA CATCAGCAGC ATCCCCGTCT CCAGCTTCAA CCACATGCCC 
AAACTCCGAA CTTTCCGTCT TCACTCCAAC AACTTAAACT GTGATTGTCA CCTGGCCTGG 
CTGTCCCAGT GGCTCAGACA GAGGCCTACG ATTGGTCTGT TCACCCAGTG TGCTTCGCCG 
CCAGAGCTAA GAGGACTCAA TGTGGCGGAG GTCCAGAAAC ACGAATTCTC CTGTTCAGGG 
CATCAGGAGT CTTCCAGCCT GCAGCCGTGC AGCATTGGCG GAGGCTCATG TCCCGCCATG 
TGCACCTGCA GTAACAACAT CGTGGACTGC AGAGGGAAAG GTCTCACTGC CATTCCAGCC 
AATCTGCCGG ACAGCATGTC AGAAATACGT CTGGAGCAGA ACGGGATTAA GTCTGTGCCT 
CCCGGAGCCT TCTCCCCCTA CAAGAAACTG CGCAGGATAG ACCTGAGCAA CAACCAGATC 
TCAGAGATAG CGCCTGACGC CTTCCAGGGA CTGCGATCTC TTAACTCTCT CGTGCTTTAC 
GGAAACAAGA TCACCGACCT GCCGAAGGGT GTGTTTGACG GCCTCTACTC TTTGCAACTG 
CTGCTGCTGA ATGCCAATAA GATTCACTGC GTCCGTGCCA ATGCCTTCCA GGATCTGCAG 
AATCTCTCCC TGCTATCGCT GTACGATAAC AAGATCCAAA CCCTCGCCAA GGGCACCTTC 
ACTTCCCTGC GAGCAATACA AACCCTGCAC CTGGCGCAGA ACCCGTTTAT TTGTGACTGT 
AATCTGAAGT GGCTGGCAGA CTACCTGCGC TCCAACCCAA TCGAGACCAG CGGCGCCCGC 
TGCGCCAGCC CACGGCGCCT CGCAAACAAA CGCATCGGAC AAATCAAGAG CAAGAAGTTC 
CGCTGCTCCG CCAAAGAGCA GTACGTCATC CCAGGTACAG AAGACACCCG TCTCAATAAC 
GCCTGCAACA CTGATCCCGT GTGTCCTCCC AAGTGTCGCT GTGAGTCTAA CGTAGTGGAC 
TGCTCCAACC TGAAACTTAC CAAGATCCCC GAGCACATCC CTGCATCCAC AGCTGAACTC 
CGCCTTAACA ATAACGAGAT CACCAGCCTT GAGGCAACTG GAGTCTTTAA GAACCTTTCC 
CATCTGAAGA AAATTAACCT GAGTAACAAC AAGATCACAG AGATCGAGGA CGGGGCTTTT 
GAAGGTGCAT CCTCTGTCAT TGAGCTCCAC CTCACAGCCA ATCAAATCGA CTCGGTTCGC 
AGCGGGATGT TCCGAGGCCT CGAGGGACTG CGCATGATGA TGCTGCGGAA CAACAAAATC 
AGCTGTGTCC ACAACGACAG CTTCACGGGG CTTCACAACG TCCGTCTGCT GTCTCTGTAC 
GACAACCACC TGACCACCAT TACTCCTGGA GCATTCGACA CGCTGCAGAC TCTCTCTACT 
CTAAACCTCT TGGCCAACTC CTTCAACTGC GACTGCCGGC TGGCGTGGCT CGGCGACTGG 
CTGAGGAGCA GGAAGATTGT GACGGGTAAC CCTCGCTGCC AGCGCCCGGC CTTCCTGAAG 
GAGATCCCCC TGCAGGATGT AGCGCTGCCG GATTTTCGCT GCGAGGAAGG CCAAGAGGAG 
TCAAGCTGTG TTCCTCGACC CCAGTGTCCC AGCGAGTGTA CTTGCCTAGA GACGGTGGTG 
CGCTGCAGCA ACAAGCATCT CCACACACTA CCCAAAGGCA TCCCCAGGAA TGTGACTGAA 
CTGTACCTTG ATGGCAACCA GTTCAGTGTT GTTCCCAAGG AACTGTCTTC CTTCAAATTC 
CTACAGCTGG TTGACCTGAG CAACAACAAA ATCAACTCTC TGACCAATTC ATCCTTTGCT 
AACATGAGCC AACTGACCAC TCTAATCCTG AGCTACAACT CCCTGCGCTG CATACCCAAA 
ATGGCTTTCA GTGGCCTGCA CTCACTGCGA CTGCTGTCAC TTCATGGTAA TGAAATCTCA 
GAGCTTCCAG ATGGCATCTT CAATGATGTG ACCTCCCTCT CCCATTTGGC GATCGGAGCC 
AACCCTCTGC ACTGTGACTG CCACCTGCGC TGGCTCTCCG ACTGGGTGAA GACCGGATAC 
AAAGAACCTG GGATCGCGCG CTGTGCCGGC CCACAGGGGA TGGAGAGCAA GCTGCTCCTC 
ACCACGCCTG CCAAGAAATT TGAATGCACA GGCGATGTGG ACACGTCGGT GCTGGCCAAG 
TGTAACCCCT GCCTGTCCAG TCCCTGCCTG AATCACGGCA TCTGTCACAA TGACCCATTG 
GAGATCTACA GGTGCAGCTG TCCTCCTGGG TTCAAGGGCA AGAACTGTGA GACGGCTGCG 
AATGCCTGTG TGAGTAGCCC ATGCGCCAAT GGAGGAACCT GCAAAGTGAA TGAGGAGGAC 
GAGGGAGAGT ACCGCTGCTC CTGTCTGCCG GGCTTCGAGG GCCCGACTTG CCAAATCAAT 
GTTGACGACT GTGAAGACAA CGACTGTGAG AACGGGGCGA CCTGCATTGA TGGAGTCAAC 
AACTACACTT GTTTTTGTCC CCCCTACTAC ACAGGGGAGA TGTGTGAAGA AATGGACGAT 
GTGTGTGCTC CAGGGCGAAG CCCCTGCCAA CATCAGTCTA CCTGCCTCAT CAGCACCTCT 
GGACCCAAGT GTGTGTGTTC TCCTGGGTAT GTTGGTGATG ACTGCAGTGT CAACTACGAT 
GACTGCAGGG AGCATCGCTG TCAGAACGGA GCACAGTGCG TGGATGAGCT GAACGGATAT 
TCCTGCATCT GCCCCGAAGG ATATAGCGGC CAGCTGTGTG AAGTTCCCCC ATCTCCCCTG 
TCACTGTGTG AGCTGGCCGA CTGCCAAAAC AACGCCCCCT GTGTGGAGCG AGGTGGCCGC 
GCCCTCTGCC AGTGTCCTCC AGAGTTCGGG GGTCCGCGCT GCGAAAAACT GGTCAGCGTC 
AACTTTGTCG ACAGAGACTC CTACTTATTG CTGTCCGACC TGGAGAACTG GCCACAAGCT 
AACATCACCC TGCAGGTTTC CACAGCTGAG GACAACGGGA TCCTGCTGTA CAATGGAGAC 
AACGATCACA TTGCAGTGGA GCTCTTCCAA GGCCACGTGA AAGTGAGCTA CGACCCGGGC 
AGCCAGCCAG GACACGCCAT ATACAGCACC GAGACCATCA ACGATGGTCA GTTCCACACA 
GTGGAGCTGG TGACCTTCGA CCAGATGGTG AACCTGTCCA TTGATGGGGG TCTCCCTACC 
ACAATGGACA GCTTTGGGGC TGTGCGGCCC CTCAACGGAG AGGCGCCACT ATACGTGGGG 
GGCATGCCTG TGGATGTCCA CTCCGGCACG TTCCGTCTCT GGCAGATCCA GAACGGCTCC 
AGCTTCCACG GCTGCATCCA GAACCTGTAC ATCAACAATG AGCTCCAGGA CTTCACCAAG 
ACCCAAATGA AGCCCGGTGT GGTGCCAGGC TGCGAGCCCT GCAAGAAGCT CTACTGTGTC 
CATGGCATCT GCCAGCCTGA CGGGTTACAG GGACCCGTGT GTCACTGCCA GCCAGGCTGG 
AGCGGGGCGC ACTGTGATCA GCCGGCCCTC AACCCCTGCC AGGGCAGCAA ATGCGTCCAT 
GGAAAGTGCA TTCCTCTGGA CTCTCAGTCT TACCGCTGCG AGTGCGAAGA GGGCTACAGA 
GGCGCGCTTT GTAACCAGCA AGGAGAGCTG TTCAACCCCT GCCGCCGCCT CTCCTGCAAA 
CACGGCCGCT GCCAGATCTC CGACACCGGA GACGCATACT GTCACTGCGA AAGCGGCTAC 
ACGGGGGAAC TCTGTGATGC AGAGTCTGAA TGTCGAGGCG AGCCGGTGAG AGACTTCTAC 
CAGGTACAGC GGGGTTACGC CATCTGCCAA ACCACCCGCA TGGTCTCCTG GGTGGAGTGC 
ACCGGAGCTT GTGACACAGG GAGCTGCTGC ATCAGCCAGA GGATGAAGCG CCGGAAATAC 
ACCTTTGAGT GCAGCGACGG AACGTCCTTC ACCGAGGAGG TGGAAAAGAC CATCAAGTGC 
GGCTGCGTGG GCTGCATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012735927.1
CDS	30..4970
Translation	MQTRANTNSTKTRQERSFQEGKKERKKETIPFFFTWTNYSGYQSCIIRSVGFVWKIFFFFLPSIYTLQLNKIVPAGLVRCGRTFLEAKRKENIKRGGQKQVRRGKKKKKRLRNMPAEDSSFESSMGRLAGQWMWLLMLSALMLGDASACPALCTCSGTTVDCHGLGLKTMPRSIPRNTERLELNGNNLTRITKSDFAGLKYLRVLQLMENQITVIERGAFDDMKELERLRLNRNQLQQLPELLFQKNPGLSRLDLSENFIQSIPRKAFRGATDIKNLQLDKNHIGCIEDGAFRALRGLEVLTLNNNNISSIPVSSFNHMPKLRTFRLHSNNLNCDCHLAWLSQWLRQRPTIGLFTQCASPPELRGLNVAEVQKHEFSCSGHQESSSLQPCSIGGGSCPAMCTCSNNIVDCRGKGLTAIPANLPDSMSEIRLEQNGIKSVPPGAFSPYKKLRRIDLSNNQISEIAPDAFQGLRSLNSLVLYGNKITDLPKGVFDGLYSLQLLLLNANKIHCVRANAFQDLQNLSLLSLYDNKIQTLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADYLRSNPIETSGARCASPRRLANKRIGQIKSKKFRCSAKEQYVIPGTEDTRLNNACNTDPVCPPKCRCESNVVDCSNLKLTKIPEHIPASTAELRLNNNEITSLEATGVFKNLSHLKKINLSNNKITEIEDGAFEGASSVIELHLTANQIDSVRSGMFRGLEGLRMMMLRNNKISCVHNDSFTGLHNVRLLSLYDNHLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTGNPRCQRPAFLKEIPLQDVALPDFRCEEGQEESSCVPRPQCPSECTCLETVVRCSNKHLHTLPKGIPRNVTELYLDGNQFSVVPKELSSFKFLQLVDLSNNKINSLTNSSFANMSQLTTLILSYNSLRCIPKMAFSGLHSLRLLSLHGNEISELPDGIFNDVTSLSHLAIGANPLHCDCHLRWLSDWVKTGYKEPGIARCAGPQGMESKLLLTTPAKKFECTGDVDTSVLAKCNPCLSSPCLNHGICHNDPLEIYRCSCPPGFKGKNCETAANACVSSPCANGGTCKVNEEDEGEYRCSCLPGFEGPTCQINVDDCEDNDCENGATCIDGVNNYTCFCPPYYTGEMCEEMDDVCAPGRSPCQHQSTCLISTSGPKCVCSPGYVGDDCSVNYDDCREHRCQNGAQCVDELNGYSCICPEGYSGQLCEVPPSPLSLCELADCQNNAPCVERGGRALCQCPPEFGGPRCEKLVSVNFVDRDSYLLLSDLENWPQANITLQVSTAEDNGILLYNGDNDHIAVELFQGHVKVSYDPGSQPGHAIYSTETINDGQFHTVELVTFDQMVNLSIDGGLPTTMDSFGAVRPLNGEAPLYVGGMPVDVHSGTFRLWQIQNGSSFHGCIQNLYINNELQDFTKTQMKPGVVPGCEPCKKLYCVHGICQPDGLQGPVCHCQPGWSGAHCDQPALNPCQGSKCVHGKCIPLDSQSYRCECEEGYRGALCNQQGELFNPCRRLSCKHGRCQISDTGDAYCHCESGYTGELCDAESECRGEPVRDFYQVQRGYAICQTTRMVSWVECTGACDTGSCCISQRMKRRKYTFECSDGTSFTEEVEKTIKCGCVGCM

Target ORF information:

RefSeq Version	XM_012880473.2
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus slit homolog 1 protein (LOC105938657), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012880473.2

ORF Insert Sequence:

ATGCAGACCA GAGCAAACAC CAACTCCACA AAGACGAGAC AAGAGAGGAG TTTTCAAGAA 
GGAAAGAAAG AAAGAAAGAA AGAAACCATC CCTTTTTTTT TTACATGGAC AAATTATAGT 
GGATATCAAA GCTGCATCAT CAGATCTGTA GGATTTGTGT GGAAGATCTT TTTTTTTTTT 
CTCCCTTCTA TTTATACTCT CCAGTTAAAT AAAATTGTTC CTGCAGGACT TGTGAGGTGT 
GGCAGGACTT TTTTGGAAGC CAAAAGGAAG GAGAACATAA AGAGAGGTGG ACAGAAACAA 
GTAAGGAGGG GAAAAAAAAA GAAGAAAAGG CTTAGGAACA TGCCCGCTGA AGACAGCAGT 
TTTGAATCAA GCATGGGCAG GCTGGCTGGG CAGTGGATGT GGCTGCTGAT GCTCTCTGCC 
TTGATGCTGG GCGATGCCAG CGCCTGCCCG GCGCTTTGTA CCTGCAGCGG CACCACAGTG 
GACTGCCACG GACTGGGGCT CAAAACTATG CCAAGGAGTA TCCCCCGCAA CACTGAGAGA 
CTGGAGCTGA ACGGAAACAA CCTCACACGC ATCACCAAGA GTGACTTTGC TGGCCTGAAG 
TACCTCAGAG TGCTACAGCT GATGGAGAAT CAGATTACAG TGATTGAGCG AGGAGCTTTC 
GATGACATGA AGGAGCTGGA GCGGCTGCGG CTGAACCGTA ATCAACTGCA GCAGCTTCCG 
GAGCTGCTGT TTCAGAAGAA CCCCGGCCTG TCTCGGCTCG ATCTGAGTGA GAACTTCATC 
CAATCCATCC CCAGAAAAGC CTTCAGAGGA GCGACAGACA TCAAAAACCT GCAGCTGGAT 
AAAAACCACA TCGGCTGCAT CGAGGATGGA GCCTTCCGAG CTCTAAGAGG CCTGGAAGTA 
CTAACGCTGA ACAACAACAA CATCAGCAGC ATCCCCGTCT CCAGCTTCAA CCACATGCCC 
AAACTCCGAA CTTTCCGTCT TCACTCCAAC AACTTAAACT GTGATTGTCA CCTGGCCTGG 
CTGTCCCAGT GGCTCAGACA GAGGCCTACG ATTGGTCTGT TCACCCAGTG TGCTTCGCCG 
CCAGAGCTAA GAGGACTCAA TGTGGCGGAG GTCCAGAAAC ACGAATTCTC CTGTTCAGGG 
CATCAGGAGT CTTCCAGCCT GCAGCCGTGC AGCATTGGCG GAGGCTCATG TCCCGCCATG 
TGCACCTGCA GTAACAACAT CGTGGACTGC AGAGGGAAAG GTCTCACTGC CATTCCAGCC 
AATCTGCCGG ACAGCATGTC AGAAATACGT CTGGAGCAGA ACGGGATTAA GTCTGTGCCT 
CCCGGAGCCT TCTCCCCCTA CAAGAAACTG CGCAGGATAG ACCTGAGCAA CAACCAGATC 
TCAGAGATAG CGCCTGACGC CTTCCAGGGA CTGCGATCTC TTAACTCTCT CGTGCTTTAC 
GGAAACAAGA TCACCGACCT GCCGAAGGGT GTGTTTGACG GCCTCTACTC TTTGCAACTG 
CTGCTGCTGA ATGCCAATAA GATTCACTGC GTCCGTGCCA ATGCCTTCCA GGATCTGCAG 
AATCTCTCCC TGCTATCGCT GTACGATAAC AAGATCCAAA CCCTCGCCAA GGGCACCTTC 
ACTTCCCTGC GAGCAATACA AACCCTGCAC CTGGCGCAGA ACCCGTTTAT TTGTGACTGT 
AATCTGAAGT GGCTGGCAGA CTACCTGCGC TCCAACCCAA TCGAGACCAG CGGCGCCCGC 
TGCGCCAGCC CACGGCGCCT CGCAAACAAA CGCATCGGAC AAATCAAGAG CAAGAAGTTC 
CGCTGCTCCG CCAAAGAGCA GTACGTCATC CCAGGTACAG AAGACACCCG TCTCAATAAC 
GCCTGCAACA CTGATCCCGT GTGTCCTCCC AAGTGTCGCT GTGAGTCTAA CGTAGTGGAC 
TGCTCCAACC TGAAACTTAC CAAGATCCCC GAGCACATCC CTGCATCCAC AGCTGAACTC 
CGCCTTAACA ATAACGAGAT CACCAGCCTT GAGGCAACTG GAGTCTTTAA GAACCTTTCC 
CATCTGAAGA AAATTAACCT GAGTAACAAC AAGATCACAG AGATCGAGGA CGGGGCTTTT 
GAAGGTGCAT CCTCTGTCAT TGAGCTCCAC CTCACAGCCA ATCAAATCGA CTCGGTTCGC 
AGCGGGATGT TCCGAGGCCT CGAGGGACTG CGCATGATGA TGCTGCGGAA CAACAAAATC 
AGCTGTGTCC ACAACGACAG CTTCACGGGG CTTCACAACG TCCGTCTGCT GTCTCTGTAC 
GACAACCACC TGACCACCAT TACTCCTGGA GCATTCGACA CGCTGCAGAC TCTCTCTACT 
CTAAACCTCT TGGCCAACTC CTTCAACTGC GACTGCCGGC TGGCGTGGCT CGGCGACTGG 
CTGAGGAGCA GGAAGATTGT GACGGGTAAC CCTCGCTGCC AGCGCCCGGC CTTCCTGAAG 
GAGATCCCCC TGCAGGATGT AGCGCTGCCG GATTTTCGCT GCGAGGAAGG CCAAGAGGAG 
TCAAGCTGTG TTCCTCGACC CCAGTGTCCC AGCGAGTGTA CTTGCCTAGA GACGGTGGTG 
CGCTGCAGCA ACAAGCATCT CCACACACTA CCCAAAGGCA TCCCCAGGAA TGTGACTGAA 
CTGTACCTTG ATGGCAACCA GTTCAGTGTT GTTCCCAAGG AACTGTCTTC CTTCAAATTC 
CTACAGCTGG TTGACCTGAG CAACAACAAA ATCAACTCTC TGACCAATTC ATCCTTTGCT 
AACATGAGCC AACTGACCAC TCTAATCCTG AGCTACAACT CCCTGCGCTG CATACCCAAA 
ATGGCTTTCA GTGGCCTGCA CTCACTGCGA CTGCTGTCAC TTCATGGTAA TGAAATCTCA 
GAGCTTCCAG ATGGCATCTT CAATGATGTG ACCTCCCTCT CCCATTTGGC GATCGGAGCC 
AACCCTCTGC ACTGTGACTG CCACCTGCGC TGGCTCTCCG ACTGGGTGAA GACCGGATAC 
AAAGAACCTG GGATCGCGCG CTGTGCCGGC CCACAGGGGA TGGAGAGCAA GCTGCTCCTC 
ACCACGCCTG CCAAGAAATT TGAATGCACA GGCGATGTGG ACACGTCGGT GCTGGCCAAG 
TGTAACCCCT GCCTGTCCAG TCCCTGCCTG AATCACGGCA TCTGTCACAA TGACCCATTG 
GAGATCTACA GGTGCAGCTG TCCTCCTGGG TTCAAGGGCA AGAACTGTGA GACGGCTGCG 
AATGCCTGTG TGAGTAGCCC ATGCGCCAAT GGAGGAACCT GCAAAGTGAA TGAGGAGGAC 
GAGGGAGAGT ACCGCTGCTC CTGTCTGCCG GGCTTCGAGG GCCCGACTTG CCAAATCAAT 
GTTGACGACT GTGAAGACAA CGACTGTGAG AACGGGGCGA CCTGCATTGA TGGAGTCAAC 
AACTACACTT GTTTTTGTCC CCCCTACTAC ACAGGGGAGA TGTGTGAAGA AATGGACGAT 
GTGTGTGCTC CAGGGCGAAG CCCCTGCCAA CATCAGTCTA CCTGCCTCAT CAGCACCTCT 
GGACCCAAGT GTGTGTGTTC TCCTGGGTAT GTTGGTGATG ACTGCAGTGT CAACTACGAT 
GACTGCAGGG AGCATCGCTG TCAGAACGGA GCACAGTGCG TGGATGAGCT GAACGGATAT 
TCCTGCATCT GCCCCGAAGG ATATAGCGGC CAGCTGTGTG AAGTTCCCCC ATCTCCCCTG 
TCACTGTGTG AGCTGGCCGA CTGCCAAAAC AACGCCCCCT GTGTGGAGCG AGGTGGCCGC 
GCCCTCTGCC AGTGTCCTCC AGAGTTCGGG GGTCCGCGCT GCGAAAAACT GGTCAGCGTC 
AACTTTGTCG ACAGAGACTC CTACTTATTG CTGTCCGACC TGGAGAACTG GCCACAAGCT 
AACATCACCC TGCAGGTTTC CACAGCTGAG GACAACGGGA TCCTGCTGTA CAATGGAGAC 
AACGATCACA TTGCAGTGGA GCTCTTCCAA GGCCACGTGA AAGTGAGCTA CGACCCGGGC 
AGCCAGCCAG GACACGCCAT ATACAGCACC GAGACCATCA ACGATGGTCA GTTCCACACA 
GTGGAGCTGG TGACCTTCGA CCAGATGGTG AACCTGTCCA TTGATGGGGG TCTCCCTACC 
ACAATGGACA GCTTTGGGGC TGTGCGGCCC CTCAACGGAG AGGCGCCACT ATACGTGGGG 
GGCATGCCTG TGGATGTCCA CTCCGGCACG TTCCGTCTCT GGCAGATCCA GAACGGCTCC 
AGCTTCCACG GCTGCATCCA GAACCTGTAC ATCAACAATG AGCTCCAGGA CTTCACCAAG 
ACCCAAATGA AGCCCGGTGT GGTGCCAGGC TGCGAGCCCT GCAAGAAGCT CTACTGTGTC 
CATGGCATCT GCCAGCCTGA CGGGTTACAG GGACCCGTGT GTCACTGCCA GCCAGGCTGG 
AGCGGGGCGC ACTGTGATCA GCCGGCCCTC AACCCCTGCC AGGGCAGCAA ATGCGTCCAT 
GGAAAGTGCA TTCCTCTGGA CTCTCAGTCT TACCGCTGCG AGTGCGAAGA GGGCTACAGA 
GGCGCGCTTT GTAACCAGCA AGGAGAGCTG TTCAACCCCT GCCGCCGCCT CTCCTGCAAA 
CACGGCCGCT GCCAGATCTC CGACACCGGA GACGCATACT GTCACTGCGA AAGCGGCTAC 
ACGGGGGAAC TCTGTGATGC AGAGTCTGAA TGTCGAGGCG AGCCGGTGAG AGACTTCTAC 
CAGGTACAGC GGGGTTACGC CATCTGCCAA ACCACCCGCA TGGTCTCCTG GGTGGAGTGC 
ACCGGAGCTT GTGACACAGG GAGCTGCTGC ATCAGCCAGA GGATGAAGCG CCGGAAATAC 
ACCTTTGAGT GCAGCGACGG AACGTCCTTC ACCGAGGAGG TGGAAAAGAC CATCAAGTGC 
GGCTGCGTGG GCTGCATGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105938657 ( XM_012880474.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012735928.1 Fundulus heteroclitus slit homolog 1 (Drosophila) (slit1), transcript variant X4, mRNA.

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CloneID	OFu124015
Clone ID Related Accession (Same CDS sequence)	XM_012880474.1
Accession Version	XM_012880474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	slit homolog 1 protein isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234518.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCA GAGCAAACAC CAACTCCACA AAGACGAGAC AAGAGAGGAG TTTTCAAGAA 
GGAAAGAAAG AAAGAAAGAA AGAAACCATC CCTTTTTTTT TTACATGGAC AAATTATAGT 
GGATATCAAA GCTGCATCAT CAGATCTGTA GGATTTGTGT GGAAGATCTT TTTTTTTTTT 
CTCCCTTCTA TTTATACTCT CCAGTTAAAT AAAATTGTTC CTGCAGGACT TGTGAGGTGT 
GGCAGGACTT TTTTGGAAGC CAAAAGGAAG GAGAACATAA AGAGAGGTGG ACAGAAACAA 
GTAAGGAGGG GAAAAAAAAA GAAGAAAAGG CTTAGGAACA TGCCCGCTGA AGACAGCAGT 
TTTGAATCAA GCATGGGCAG GCTGGCTGGG CAGTGGATGT GGCTGCTGAT GCTCTCTGCC 
TTGATGCTGG GCGATGCCAG CGCCTGCCCG GCGCTTTGTA CCTGCAGCGG CACCACAGTG 
GACTGCCACG GACTGGGGCT CAAAACTATG CCAAGGAGTA TCCCCCGCAA CACTGAGAGA 
CTGGAGCTGA ACGGAAACAA CCTCACACGC ATCACCAAGA GTGACTTTGC TGGCCTGAAG 
TACCTCAGAG TGCTACAGCT GATGGAGAAT CAGATTACAG TGATTGAGCG AGGAGCTTTC 
GATGACATGA AGGAGCTGGA GCGGCTGCGG CTGAACCGTA ATCAACTGCA GCAGCTTCCG 
GAGCTGCTGT TTCAGAAGAA CCCCGGCCTG TCTCGGCTCG ATCTGAGTGA GAACTTCATC 
CAATCCATCC CCAGAAAAGC CTTCAGAGGA GCGACAGACA TCAAAAACCT GCAGCTGGAT 
AAAAACCACA TCGGCTGCAT CGAGGATGGA GCCTTCCGAG CTCTAAGAGG CCTGGAAGTA 
CTAACGCTGA ACAACAACAA CATCAGCAGC ATCCCCGTCT CCAGCTTCAA CCACATGCCC 
AAACTCCGAA CTTTCCGTCT TCACTCCAAC AACTTAAACT GTGATTGTCA CCTGGCCTGG 
CTGTCCCAGT GGCTCAGACA GAGGCCTACG ATTGGTCTGT TCACCCAGTG TGCTTCGCCG 
CCAGAGCTAA GAGGACTCAA TGTGGCGGAG GTCCAGAAAC ACGAATTCTC CTGTTCAGGG 
CATCAGGAGT CTTCCAGCCT GCAGCCGTGC AGCATTGGCG GAGGCTCATG TCCCGCCATG 
TGCACCTGCA GTAACAACAT CGTGGACTGC AGAGGGAAAG GTCTCACTGC CATTCCAGCC 
AATCTGCCGG ACAGCATGTC AGAAATACGT CTGGAGCAGA ACGGGATTAA GTCTGTGCCT 
CCCGGAGCCT TCTCCCCCTA CAAGAAACTG CGCAGGATAG ACCTGAGCAA CAACCAGATC 
TCAGAGATAG CGCCTGACGC CTTCCAGGGA CTGCGATCTC TTAACTCTCT CGTGCTTTAC 
GGAAACAAGA TCACCGACCT GCCGAAGGGT GTGTTTGACG GCCTCTACTC TTTGCAACTG 
CTGCTGCTGA ATGCCAATAA GATTCACTGC GTCCGTGCCA ATGCCTTCCA GGATCTGCAG 
AATCTCTCCC TGCTATCGCT GTACGATAAC AAGATCCAAA CCCTCGCCAA GGGCACCTTC 
ACTTCCCTGC GAGCAATACA AACCCTGCAC CTGGCGCAGA ACCCGTTTAT TTGTGACTGT 
AATCTGAAGT GGCTGGCAGA CTACCTGCGC TCCAACCCAA TCGAGACCAG CGGCGCCCGC 
TGCGCCAGCC CACGGCGCCT CGCAAACAAA CGCATCGGAC AAATCAAGAG CAAGAAGTTC 
CGCTGCTCCG GTACAGAAGA CACCCGTCTC AATAACGCCT GCAACACTGA TCCCGTGTGT 
CCTCCCAAGT GTCGCTGTGA GTCTAACGTA GTGGACTGCT CCAACCTGAA ACTTACCAAG 
ATCCCCGAGC ACATCCCTGC ATCCACAGCT GAACTCCGCC TTAACAATAA CGAGATCACC 
AGCCTTGAGG CAACTGGAGT CTTTAAGAAC CTTTCCCATC TGAAGAAAAT TAACCTGAGT 
AACAACAAGA TCACAGAGAT CGAGGACGGG GCTTTTGAAG GTGCATCCTC TGTCATTGAG 
CTCCACCTCA CAGCCAATCA AATCGACTCG GTTCGCAGCG GGATGTTCCG AGGCCTCGAG 
GGACTGCGCA TGATGATGCT GCGGAACAAC AAAATCAGCT GTGTCCACAA CGACAGCTTC 
ACGGGGCTTC ACAACGTCCG TCTGCTGTCT CTGTACGACA ACCACCTGAC CACCATTACT 
CCTGGAGCAT TCGACACGCT GCAGACTCTC TCTACTCTAA ACCTCTTGGC CAACTCCTTC 
AACTGCGACT GCCGGCTGGC GTGGCTCGGC GACTGGCTGA GGAGCAGGAA GATTGTGACG 
GGTAACCCTC GCTGCCAGCG CCCGGCCTTC CTGAAGGAGA TCCCCCTGCA GGATGTAGCG 
CTGCCGGATT TTCGCTGCGA GGAAGGCCAA GAGGAGTCAA GCTGTGTTCC TCGACCCCAG 
TGTCCCAGCG AGTGTACTTG CCTAGAGACG GTGGTGCGCT GCAGCAACAA GCATCTCCAC 
ACACTACCCA AAGGCATCCC CAGGAATGTG ACTGAACTGT ACCTTGATGG CAACCAGTTC 
AGTGTTGTTC CCAAGGAACT GTCTTCCTTC AAATTCCTAC AGCTGGTTGA CCTGAGCAAC 
AACAAAATCA ACTCTCTGAC CAATTCATCC TTTGCTAACA TGAGCCAACT GACCACTCTA 
ATCCTGAGCT ACAACTCCCT GCGCTGCATA CCCAAAATGG CTTTCAGTGG CCTGCACTCA 
CTGCGACTGC TGTCACTTCA TGGTAATGAA ATCTCAGAGC TTCCAGATGG CATCTTCAAT 
GATGTGACCT CCCTCTCCCA TTTGGCGATC GGAGCCAACC CTCTGCACTG TGACTGCCAC 
CTGCGCTGGC TCTCCGACTG GGTGAAGACC GGATACAAAG AACCTGGGAT CGCGCGCTGT 
GCCGGCCCAC AGGGGATGGA GAGCAAGCTG CTCCTCACCA CGCCTGCCAA GAAATTTGAA 
TGCACAGGCG ATGTGGACAC GTCGGTGCTG GCCAAGTGTA ACCCCTGCCT GTCCAGTCCC 
TGCCTGAATC ACGGCATCTG TCACAATGAC CCATTGGAGA TCTACAGGTG CAGCTGTCCT 
CCTGGGTTCA AGGGCAAGAA CTGTGAGACG GCTGCGAATG CCTGTGTGAG TAGCCCATGC 
GCCAATGGAG GAACCTGCAA AGTGAATGAG GAGGACGAGG GAGAGTACCG CTGCTCCTGT 
CTGCCGGGCT TCGAGGGCCC GACTTGCCAA ATCAATGTTG ACGACTGTGA AGACAACGAC 
TGTGAGAACG GGGCGACCTG CATTGATGGA GTCAACAACT ACACTTGTTT TTGTCCCCCC 
TACTACACAG GGGAGATGTG TGAAGAAATG GACGATGTGT GTGCTCCAGG GCGAAGCCCC 
TGCCAACATC AGTCTACCTG CCTCATCAGC ACCTCTGGAC CCAAGTGTGT GTGTTCTCCT 
GGGTATGTTG GTGATGACTG CAGTGTCAAC TACGATGACT GCAGGGAGCA TCGCTGTCAG 
AACGGAGCAC AGTGCGTGGA TGAGCTGAAC GGATATTCCT GCATCTGCCC CGAAGGATAT 
AGCGGCCAGC TGTGTGAAGT TCCCCCATCT CCCCTGTCAC TGTGTGAGCT GGCCGACTGC 
CAAAACAACG CCCCCTGTGT GGAGCGAGGT GGCCGCGCCC TCTGCCAGTG TCCTCCAGAG 
TTCGGGGGTC CGCGCTGCGA AAAACTGGTC AGCGTCAACT TTGTCGACAG AGACTCCTAC 
TTATTGCTGT CCGACCTGGA GAACTGGCCA CAAGCTAACA TCACCCTGCA GGTTTCCACA 
GCTGAGGACA ACGGGATCCT GCTGTACAAT GGAGACAACG ATCACATTGC AGTGGAGCTC 
TTCCAAGGCC ACGTGAAAGT GAGCTACGAC CCGGGCAGCC AGCCAGGACA CGCCATATAC 
AGCACCGAGA CCATCAACGA TGGTCAGTTC CACACAGTGG AGCTGGTGAC CTTCGACCAG 
ATGGTGAACC TGTCCATTGA TGGGGGTCTC CCTACCACAA TGGACAGCTT TGGGGCTGTG 
CGGCCCCTCA ACGGAGAGGC GCCACTATAC GTGGGGGGCA TGCCTGTGGA TGTCCACTCC 
GGCACGTTCC GTCTCTGGCA GATCCAGAAC GGCTCCAGCT TCCACGGCTG CATCCAGAAC 
CTGTACATCA ACAATGAGCT CCAGGACTTC ACCAAGACCC AAATGAAGCC CGGTGTGGTG 
CCAGGCTGCG AGCCCTGCAA GAAGCTCTAC TGTGTCCATG GCATCTGCCA GCCTGACGGG 
TTACAGGGAC CCGTGTGTCA CTGCCAGCCA GGCTGGAGCG GGGCGCACTG TGATCAGCCG 
GCCCTCAACC CCTGCCAGGG CAGCAAATGC GTCCATGGAA AGTGCATTCC TCTGGACTCT 
CAGTCTTACC GCTGCGAGTG CGAAGAGGGC TACAGAGGCG CGCTTTGTAA CCAGCAAGGA 
GAGCTGTTCA ACCCCTGCCG CCGCCTCTCC TGCAAACACG GCCGCTGCCA GATCTCCGAC 
ACCGGAGACG CATACTGTCA CTGCGAAAGC GGCTACACGG GGGAACTCTG TGATGCAGAG 
TCTGAATGTC GAGGCGAGCC GGTGAGAGAC TTCTACCAGG TACAGCGGGG TTACGCCATC 
TGCCAAACCA CCCGCATGGT CTCCTGGGTG GAGTGCACCG GAGCTTGTGA CACAGGGAGC 
TGCTGCATCA GCCAGAGGAT GAAGCGCCGG AAATACACCT TTGAGTGCAG CGACGGAACG 
TCCTTCACCG AGGAGGTGGA AAAGACCATC AAGTGCGGCT GCGTGGGCTG CATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012735928.1
CDS	109..5025
Translation	MQTRANTNSTKTRQERSFQEGKKERKKETIPFFFTWTNYSGYQSCIIRSVGFVWKIFFFFLPSIYTLQLNKIVPAGLVRCGRTFLEAKRKENIKRGGQKQVRRGKKKKKRLRNMPAEDSSFESSMGRLAGQWMWLLMLSALMLGDASACPALCTCSGTTVDCHGLGLKTMPRSIPRNTERLELNGNNLTRITKSDFAGLKYLRVLQLMENQITVIERGAFDDMKELERLRLNRNQLQQLPELLFQKNPGLSRLDLSENFIQSIPRKAFRGATDIKNLQLDKNHIGCIEDGAFRALRGLEVLTLNNNNISSIPVSSFNHMPKLRTFRLHSNNLNCDCHLAWLSQWLRQRPTIGLFTQCASPPELRGLNVAEVQKHEFSCSGHQESSSLQPCSIGGGSCPAMCTCSNNIVDCRGKGLTAIPANLPDSMSEIRLEQNGIKSVPPGAFSPYKKLRRIDLSNNQISEIAPDAFQGLRSLNSLVLYGNKITDLPKGVFDGLYSLQLLLLNANKIHCVRANAFQDLQNLSLLSLYDNKIQTLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADYLRSNPIETSGARCASPRRLANKRIGQIKSKKFRCSGTEDTRLNNACNTDPVCPPKCRCESNVVDCSNLKLTKIPEHIPASTAELRLNNNEITSLEATGVFKNLSHLKKINLSNNKITEIEDGAFEGASSVIELHLTANQIDSVRSGMFRGLEGLRMMMLRNNKISCVHNDSFTGLHNVRLLSLYDNHLTTITPGAFDTLQTLSTLNLLANSFNCDCRLAWLGDWLRSRKIVTGNPRCQRPAFLKEIPLQDVALPDFRCEEGQEESSCVPRPQCPSECTCLETVVRCSNKHLHTLPKGIPRNVTELYLDGNQFSVVPKELSSFKFLQLVDLSNNKINSLTNSSFANMSQLTTLILSYNSLRCIPKMAFSGLHSLRLLSLHGNEISELPDGIFNDVTSLSHLAIGANPLHCDCHLRWLSDWVKTGYKEPGIARCAGPQGMESKLLLTTPAKKFECTGDVDTSVLAKCNPCLSSPCLNHGICHNDPLEIYRCSCPPGFKGKNCETAANACVSSPCANGGTCKVNEEDEGEYRCSCLPGFEGPTCQINVDDCEDNDCENGATCIDGVNNYTCFCPPYYTGEMCEEMDDVCAPGRSPCQHQSTCLISTSGPKCVCSPGYVGDDCSVNYDDCREHRCQNGAQCVDELNGYSCICPEGYSGQLCEVPPSPLSLCELADCQNNAPCVERGGRALCQCPPEFGGPRCEKLVSVNFVDRDSYLLLSDLENWPQANITLQVSTAEDNGILLYNGDNDHIAVELFQGHVKVSYDPGSQPGHAIYSTETINDGQFHTVELVTFDQMVNLSIDGGLPTTMDSFGAVRPLNGEAPLYVGGMPVDVHSGTFRLWQIQNGSSFHGCIQNLYINNELQDFTKTQMKPGVVPGCEPCKKLYCVHGICQPDGLQGPVCHCQPGWSGAHCDQPALNPCQGSKCVHGKCIPLDSQSYRCECEEGYRGALCNQQGELFNPCRRLSCKHGRCQISDTGDAYCHCESGYTGELCDAESECRGEPVRDFYQVQRGYAICQTTRMVSWVECTGACDTGSCCISQRMKRRKYTFECSDGTSFTEEVEKTIKCGCVGCM

Target ORF information:

RefSeq Version	XM_012880474.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus slit homolog 1 (Drosophila) (slit1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012880474.1

ORF Insert Sequence:

ATGCAGACCA GAGCAAACAC CAACTCCACA AAGACGAGAC AAGAGAGGAG TTTTCAAGAA 
GGAAAGAAAG AAAGAAAGAA AGAAACCATC CCTTTTTTTT TTACATGGAC AAATTATAGT 
GGATATCAAA GCTGCATCAT CAGATCTGTA GGATTTGTGT GGAAGATCTT TTTTTTTTTT 
CTCCCTTCTA TTTATACTCT CCAGTTAAAT AAAATTGTTC CTGCAGGACT TGTGAGGTGT 
GGCAGGACTT TTTTGGAAGC CAAAAGGAAG GAGAACATAA AGAGAGGTGG ACAGAAACAA 
GTAAGGAGGG GAAAAAAAAA GAAGAAAAGG CTTAGGAACA TGCCCGCTGA AGACAGCAGT 
TTTGAATCAA GCATGGGCAG GCTGGCTGGG CAGTGGATGT GGCTGCTGAT GCTCTCTGCC 
TTGATGCTGG GCGATGCCAG CGCCTGCCCG GCGCTTTGTA CCTGCAGCGG CACCACAGTG 
GACTGCCACG GACTGGGGCT CAAAACTATG CCAAGGAGTA TCCCCCGCAA CACTGAGAGA 
CTGGAGCTGA ACGGAAACAA CCTCACACGC ATCACCAAGA GTGACTTTGC TGGCCTGAAG 
TACCTCAGAG TGCTACAGCT GATGGAGAAT CAGATTACAG TGATTGAGCG AGGAGCTTTC 
GATGACATGA AGGAGCTGGA GCGGCTGCGG CTGAACCGTA ATCAACTGCA GCAGCTTCCG 
GAGCTGCTGT TTCAGAAGAA CCCCGGCCTG TCTCGGCTCG ATCTGAGTGA GAACTTCATC 
CAATCCATCC CCAGAAAAGC CTTCAGAGGA GCGACAGACA TCAAAAACCT GCAGCTGGAT 
AAAAACCACA TCGGCTGCAT CGAGGATGGA GCCTTCCGAG CTCTAAGAGG CCTGGAAGTA 
CTAACGCTGA ACAACAACAA CATCAGCAGC ATCCCCGTCT CCAGCTTCAA CCACATGCCC 
AAACTCCGAA CTTTCCGTCT TCACTCCAAC AACTTAAACT GTGATTGTCA CCTGGCCTGG 
CTGTCCCAGT GGCTCAGACA GAGGCCTACG ATTGGTCTGT TCACCCAGTG TGCTTCGCCG 
CCAGAGCTAA GAGGACTCAA TGTGGCGGAG GTCCAGAAAC ACGAATTCTC CTGTTCAGGG 
CATCAGGAGT CTTCCAGCCT GCAGCCGTGC AGCATTGGCG GAGGCTCATG TCCCGCCATG 
TGCACCTGCA GTAACAACAT CGTGGACTGC AGAGGGAAAG GTCTCACTGC CATTCCAGCC 
AATCTGCCGG ACAGCATGTC AGAAATACGT CTGGAGCAGA ACGGGATTAA GTCTGTGCCT 
CCCGGAGCCT TCTCCCCCTA CAAGAAACTG CGCAGGATAG ACCTGAGCAA CAACCAGATC 
TCAGAGATAG CGCCTGACGC CTTCCAGGGA CTGCGATCTC TTAACTCTCT CGTGCTTTAC 
GGAAACAAGA TCACCGACCT GCCGAAGGGT GTGTTTGACG GCCTCTACTC TTTGCAACTG 
CTGCTGCTGA ATGCCAATAA GATTCACTGC GTCCGTGCCA ATGCCTTCCA GGATCTGCAG 
AATCTCTCCC TGCTATCGCT GTACGATAAC AAGATCCAAA CCCTCGCCAA GGGCACCTTC 
ACTTCCCTGC GAGCAATACA AACCCTGCAC CTGGCGCAGA ACCCGTTTAT TTGTGACTGT 
AATCTGAAGT GGCTGGCAGA CTACCTGCGC TCCAACCCAA TCGAGACCAG CGGCGCCCGC 
TGCGCCAGCC CACGGCGCCT CGCAAACAAA CGCATCGGAC AAATCAAGAG CAAGAAGTTC 
CGCTGCTCCG GTACAGAAGA CACCCGTCTC AATAACGCCT GCAACACTGA TCCCGTGTGT 
CCTCCCAAGT GTCGCTGTGA GTCTAACGTA GTGGACTGCT CCAACCTGAA ACTTACCAAG 
ATCCCCGAGC ACATCCCTGC ATCCACAGCT GAACTCCGCC TTAACAATAA CGAGATCACC 
AGCCTTGAGG CAACTGGAGT CTTTAAGAAC CTTTCCCATC TGAAGAAAAT TAACCTGAGT 
AACAACAAGA TCACAGAGAT CGAGGACGGG GCTTTTGAAG GTGCATCCTC TGTCATTGAG 
CTCCACCTCA CAGCCAATCA AATCGACTCG GTTCGCAGCG GGATGTTCCG AGGCCTCGAG 
GGACTGCGCA TGATGATGCT GCGGAACAAC AAAATCAGCT GTGTCCACAA CGACAGCTTC 
ACGGGGCTTC ACAACGTCCG TCTGCTGTCT CTGTACGACA ACCACCTGAC CACCATTACT 
CCTGGAGCAT TCGACACGCT GCAGACTCTC TCTACTCTAA ACCTCTTGGC CAACTCCTTC 
AACTGCGACT GCCGGCTGGC GTGGCTCGGC GACTGGCTGA GGAGCAGGAA GATTGTGACG 
GGTAACCCTC GCTGCCAGCG CCCGGCCTTC CTGAAGGAGA TCCCCCTGCA GGATGTAGCG 
CTGCCGGATT TTCGCTGCGA GGAAGGCCAA GAGGAGTCAA GCTGTGTTCC TCGACCCCAG 
TGTCCCAGCG AGTGTACTTG CCTAGAGACG GTGGTGCGCT GCAGCAACAA GCATCTCCAC 
ACACTACCCA AAGGCATCCC CAGGAATGTG ACTGAACTGT ACCTTGATGG CAACCAGTTC 
AGTGTTGTTC CCAAGGAACT GTCTTCCTTC AAATTCCTAC AGCTGGTTGA CCTGAGCAAC 
AACAAAATCA ACTCTCTGAC CAATTCATCC TTTGCTAACA TGAGCCAACT GACCACTCTA 
ATCCTGAGCT ACAACTCCCT GCGCTGCATA CCCAAAATGG CTTTCAGTGG CCTGCACTCA 
CTGCGACTGC TGTCACTTCA TGGTAATGAA ATCTCAGAGC TTCCAGATGG CATCTTCAAT 
GATGTGACCT CCCTCTCCCA TTTGGCGATC GGAGCCAACC CTCTGCACTG TGACTGCCAC 
CTGCGCTGGC TCTCCGACTG GGTGAAGACC GGATACAAAG AACCTGGGAT CGCGCGCTGT 
GCCGGCCCAC AGGGGATGGA GAGCAAGCTG CTCCTCACCA CGCCTGCCAA GAAATTTGAA 
TGCACAGGCG ATGTGGACAC GTCGGTGCTG GCCAAGTGTA ACCCCTGCCT GTCCAGTCCC 
TGCCTGAATC ACGGCATCTG TCACAATGAC CCATTGGAGA TCTACAGGTG CAGCTGTCCT 
CCTGGGTTCA AGGGCAAGAA CTGTGAGACG GCTGCGAATG CCTGTGTGAG TAGCCCATGC 
GCCAATGGAG GAACCTGCAA AGTGAATGAG GAGGACGAGG GAGAGTACCG CTGCTCCTGT 
CTGCCGGGCT TCGAGGGCCC GACTTGCCAA ATCAATGTTG ACGACTGTGA AGACAACGAC 
TGTGAGAACG GGGCGACCTG CATTGATGGA GTCAACAACT ACACTTGTTT TTGTCCCCCC 
TACTACACAG GGGAGATGTG TGAAGAAATG GACGATGTGT GTGCTCCAGG GCGAAGCCCC 
TGCCAACATC AGTCTACCTG CCTCATCAGC ACCTCTGGAC CCAAGTGTGT GTGTTCTCCT 
GGGTATGTTG GTGATGACTG CAGTGTCAAC TACGATGACT GCAGGGAGCA TCGCTGTCAG 
AACGGAGCAC AGTGCGTGGA TGAGCTGAAC GGATATTCCT GCATCTGCCC CGAAGGATAT 
AGCGGCCAGC TGTGTGAAGT TCCCCCATCT CCCCTGTCAC TGTGTGAGCT GGCCGACTGC 
CAAAACAACG CCCCCTGTGT GGAGCGAGGT GGCCGCGCCC TCTGCCAGTG TCCTCCAGAG 
TTCGGGGGTC CGCGCTGCGA AAAACTGGTC AGCGTCAACT TTGTCGACAG AGACTCCTAC 
TTATTGCTGT CCGACCTGGA GAACTGGCCA CAAGCTAACA TCACCCTGCA GGTTTCCACA 
GCTGAGGACA ACGGGATCCT GCTGTACAAT GGAGACAACG ATCACATTGC AGTGGAGCTC 
TTCCAAGGCC ACGTGAAAGT GAGCTACGAC CCGGGCAGCC AGCCAGGACA CGCCATATAC 
AGCACCGAGA CCATCAACGA TGGTCAGTTC CACACAGTGG AGCTGGTGAC CTTCGACCAG 
ATGGTGAACC TGTCCATTGA TGGGGGTCTC CCTACCACAA TGGACAGCTT TGGGGCTGTG 
CGGCCCCTCA ACGGAGAGGC GCCACTATAC GTGGGGGGCA TGCCTGTGGA TGTCCACTCC 
GGCACGTTCC GTCTCTGGCA GATCCAGAAC GGCTCCAGCT TCCACGGCTG CATCCAGAAC 
CTGTACATCA ACAATGAGCT CCAGGACTTC ACCAAGACCC AAATGAAGCC CGGTGTGGTG 
CCAGGCTGCG AGCCCTGCAA GAAGCTCTAC TGTGTCCATG GCATCTGCCA GCCTGACGGG 
TTACAGGGAC CCGTGTGTCA CTGCCAGCCA GGCTGGAGCG GGGCGCACTG TGATCAGCCG 
GCCCTCAACC CCTGCCAGGG CAGCAAATGC GTCCATGGAA AGTGCATTCC TCTGGACTCT 
CAGTCTTACC GCTGCGAGTG CGAAGAGGGC TACAGAGGCG CGCTTTGTAA CCAGCAAGGA 
GAGCTGTTCA ACCCCTGCCG CCGCCTCTCC TGCAAACACG GCCGCTGCCA GATCTCCGAC 
ACCGGAGACG CATACTGTCA CTGCGAAAGC GGCTACACGG GGGAACTCTG TGATGCAGAG 
TCTGAATGTC GAGGCGAGCC GGTGAGAGAC TTCTACCAGG TACAGCGGGG TTACGCCATC 
TGCCAAACCA CCCGCATGGT CTCCTGGGTG GAGTGCACCG GAGCTTGTGA CACAGGGAGC 
TGCTGCATCA GCCAGAGGAT GAAGCGCCGG AAATACACCT TTGAGTGCAG CGACGGAACG 
TCCTTCACCG AGGAGGTGGA AAAGACCATC AAGTGCGGCT GCGTGGGCTG CATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

LOC105938657 cDNA ORF clone, Fundulus heteroclitus(mummichog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム