PLD2 cDNA ORF clone, Fundulus heteroclitus(Mummichog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLD2
Entrez Gene ID	105929401
Full Name	phospholipase D1
Synonyms	pld2
Gene Type	protein-coding
Organism	Fundulus heteroclitus(Mummichog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021318794.1	XP_021174469.1	phospholipase D1
XM_012867166.1	XP_012722620.1	phospholipase D2

Pathways from BioSystems
Gene Ontology

The following PLD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFu113160	XM_012867166.1 Latest version!	Fundulus heteroclitus phospholipase D2 (pld2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OFu138388	XM_021318794.1 Latest version!	Fundulus heteroclitus phospholipase D1 (LOC105929401), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLD2 ( XM_012867166.1 ) cDNA ORF clone, Fundulus heteroclitus(Mummichog) -> XP_012722620.1 Fundulus heteroclitus phospholipase D2 (pld2), mRNA.

Price & Availability↑

CloneID	OFu113160
Clone ID Related Accession (Same CDS sequence)	XM_012867166.1
Accession Version	XM_012867166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	phospholipase D2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234309.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAG CTGGTGTATC TTACTTCATA CCGGGTACTC CTATCACATG CAAGGTGGAC 
AACACAGAGA AACACACCAC TCGCTCAAAG GTGCGTGTGG GAACCCTGTA CACAGTGCGG 
TTAACTCATG GACACTTCCA CTGGGTAGTG AAGCGGAAGT ACAAGCACTT TCAGGAGCTT 
CACCGGGACC TTTACAAACA CAAGATGATG CTTCACTATC TGCCTTTCCA AAGGTTCGCA 
AAGGACAGGC AGCAGCTGAT GATGACGTCA GAGGAAATGC CCAGCCTACA CGGAACAGAT 
CGCACCAGAA GAACCTCAAG CAAAATGAAA TACCTTGAGG AGTACCTGAA TGGGCTGCTG 
GAGAATGCAT TCTGCAGATC TATCCACAGC ATGCTTGAGT TCCTCTCTGT CAGTGCCCTC 
TCTTTTGTCA ATGATCTGGG ACCCAAAGGC CTGGAGGGGC CGATCATGAA AAGGTCTGGC 
GGTCATCGGG TTCTGGGTCT GAATTGCATC GGTCACCATC GGGTCTGTTT TCGCTGGTCG 
CACCGCTGGC TAGTGGTCAA AGAGTCCTTC CTCTTGTACA TGAGCCGAGA CGACTCAGAA 
ATCGACTTTG TGCTGCTTTA TGACCCGGAG TTTGCCGTAA AAGTGGGCAA TGCTCATACA 
GGGACAGAGT ATGGAGTTTG CATTGAGAAC TACACACGGA GCTTAGTAAT CAAGTGCATC 
AGCTACAGAC AAAGTCAGTG GTGGAGCCAT GAAATTGACC GGCTGGCTCA CTTGTGTGAC 
TTCCTAAAAG TGAGCCGCTT TGGAGGATTC GCTCCACCAC GAGAAGATAC TCTTACTAAA 
TGGTATGTGA ACGGGAAAGA CTACTTTGCA GACCTGGCTG ATGCCCTTGA ACAAGCTAAG 
GAGGAGATTT TCATCACGGA TTGGTGGCTC AGTCCTGAAA TTTTTCTAAA GAGACCCGCC 
ACTGACAACC ACTGGAGACT GGACGAGATA CTTAAACGGA AAGCAGAACA AGGAGTCAAA 
GTATGTGTCC TGCTTTACAA GGAGGTGGAG ATGGCGCTTG GTATCAATAG TGACTACAGC 
AAGAGGACTC TGATGGACAT GCACCCAAAC ATCAAGGTGA TGCGACATCC AGACCACGTC 
TCATCTGCGG TGGTCTTGTG GGCTCACCAT GAAAAGATGG TGGCTATTGA CCAAACCGTG 
GCGTTTGTAG GGGGACTTGA CCTGGCGTTT GGCAGGTGGG ATGACAGAGA GTATCGGCTG 
GCTGACCTGG GTCAGGCAGA GTCGTTAAAC GGGGAAACGG CGGAGCATCC AAACACAGAT 
GCAACTGACA ATGGTGTGGG TGATGGCCAA AACGCAGGTA ACCAAGCAGA AAAGGACCAA 
CCTGATCTGA CTGGCAACAC TAAGCTTTGG CTGGGGAAAG ACTACAGCAA CTTCATCAAC 
AAAGACTGGA CCCTACTGGA CAAGCCGTTT GAAGATAACA TCGACCGCAC TAAAGTTCCT 
CGCATGCCTT GGCGGGACCT GTCTTCAGCT ATGCACGGCA AAGCGGCCAG GGATGTTGCC 
CGACACTTCA TCCAGCGCTG GAACTTCACA AAGATCTTCA AGACCAAGTA CAAGGACAAC 
TTCTACCCCT ACCTTCTCCC CAAGTCTAAC TCGACGGCAG ATTTGCTGCC ATTCACTATC 
CCTAGGTCCC AGAAAGCCAA AGTTCAGGTA CTGCGCTCTG CTGATCGGTG GTCAACTGGA 
ACAGCAGAGA ACTCAATCCT TAATGCCTAC ATACACACAA TTGAGAACAG CGAGCACTAC 
ATCTACATCG AGAACCAGTT TTTCATCAGC TGTGCCGACG ACAAGGTCGT CCAAAATAGA 
ATCGGGGATG CAATTGTGAA ACGAATCCTG CGAGCACACA GAGAGCAGAA GAAGTACAGG 
GTGTTTGTGG TGATTCCTTT ACTTCCTGGG TTTGAGGGAG ACATTACCAC GGGAGGTGGA 
AATGCAATTC AGGCGATTAT GCACTTCACT TACAGGACTA TGTGCCGTGG AGATCACTCA 
ATTCTGTCGA GGCTTAGTGA AGTAAAGGAC CAGTGGACTG AGTACATCAG CTTTTGTGGC 
CTCAGAACGC ATTCCCGTCT CTCTGGGTCG CTCGTCACAG AGCTGATCTA TGTCCACAGC 
AAGGCGCTCA TTGCTGATGA CCGCTGCTAT ATAATTGGAT CAGCCAACAT CAATGATCGC 
AGCATGCTGG GCACCCGAGA TAGTGAGCTG GCTGTGTTTG TGGAAGATGA AGAGAGGGTC 
CCATCCATCA TGGGAGGACA GGAATACGAG GCAGGACCGC TCGCGCTTGC TCTACGCAAA 
GAGTGCTTCA GTGTCCTGGT TGGAGCCGTA TCAGACCCCA GCATCAACAT TGACGATCCG 
ATCAGTGACG ACTTCTTCTT CCTGACCTGG AATGAAACGG CTAAACTCAA CACGGAGATC 
TACGCTAAGG TCTTCCGATG CCTCCCCTGC AACAGTGTTC ACAACATGCA GCGCCTGAGT 
GAGCACAACT GCGAAGAACG CCTCTGCGAC ACCGACCCCG AGCGGGCGGC GGAGGAGCTG 
AAGGCCGTCA GAGGGGTGCT GGTCCACTTT CCGCTGAAAT ACATGTGCGA GGAAAACCTG 
CTGCCGCCTC TGAACTGTAA AGAAGGCATG GCTCCTACAG ACCTTTGGAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012722620.1
CDS	34..2727
Translation	MKEAGVSYFIPGTPITCKVDNTEKHTTRSKVRVGTLYTVRLTHGHFHWVVKRKYKHFQELHRDLYKHKMMLHYLPFQRFAKDRQQLMMTSEEMPSLHGTDRTRRTSSKMKYLEEYLNGLLENAFCRSIHSMLEFLSVSALSFVNDLGPKGLEGPIMKRSGGHRVLGLNCIGHHRVCFRWSHRWLVVKESFLLYMSRDDSEIDFVLLYDPEFAVKVGNAHTGTEYGVCIENYTRSLVIKCISYRQSQWWSHEIDRLAHLCDFLKVSRFGGFAPPREDTLTKWYVNGKDYFADLADALEQAKEEIFITDWWLSPEIFLKRPATDNHWRLDEILKRKAEQGVKVCVLLYKEVEMALGINSDYSKRTLMDMHPNIKVMRHPDHVSSAVVLWAHHEKMVAIDQTVAFVGGLDLAFGRWDDREYRLADLGQAESLNGETAEHPNTDATDNGVGDGQNAGNQAEKDQPDLTGNTKLWLGKDYSNFINKDWTLLDKPFEDNIDRTKVPRMPWRDLSSAMHGKAARDVARHFIQRWNFTKIFKTKYKDNFYPYLLPKSNSTADLLPFTIPRSQKAKVQVLRSADRWSTGTAENSILNAYIHTIENSEHYIYIENQFFISCADDKVVQNRIGDAIVKRILRAHREQKKYRVFVVIPLLPGFEGDITTGGGNAIQAIMHFTYRTMCRGDHSILSRLSEVKDQWTEYISFCGLRTHSRLSGSLVTELIYVHSKALIADDRCYIIGSANINDRSMLGTRDSELAVFVEDEERVPSIMGGQEYEAGPLALALRKECFSVLVGAVSDPSINIDDPISDDFFFLTWNETAKLNTEIYAKVFRCLPCNSVHNMQRLSEHNCEERLCDTDPERAAEELKAVRGVLVHFPLKYMCEENLLPPLNCKEGMAPTDLWT

Target ORF information:

RefSeq Version	XM_012867166.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus phospholipase D2 (pld2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012867166.1

ORF Insert Sequence:

ATGAAGGAAG CTGGTGTATC TTACTTCATA CCGGGTACTC CTATCACATG CAAGGTGGAC 
AACACAGAGA AACACACCAC TCGCTCAAAG GTGCGTGTGG GAACCCTGTA CACAGTGCGG 
TTAACTCATG GACACTTCCA CTGGGTAGTG AAGCGGAAGT ACAAGCACTT TCAGGAGCTT 
CACCGGGACC TTTACAAACA CAAGATGATG CTTCACTATC TGCCTTTCCA AAGGTTCGCA 
AAGGACAGGC AGCAGCTGAT GATGACGTCA GAGGAAATGC CCAGCCTACA CGGAACAGAT 
CGCACCAGAA GAACCTCAAG CAAAATGAAA TACCTTGAGG AGTACCTGAA TGGGCTGCTG 
GAGAATGCAT TCTGCAGATC TATCCACAGC ATGCTTGAGT TCCTCTCTGT CAGTGCCCTC 
TCTTTTGTCA ATGATCTGGG ACCCAAAGGC CTGGAGGGGC CGATCATGAA AAGGTCTGGC 
GGTCATCGGG TTCTGGGTCT GAATTGCATC GGTCACCATC GGGTCTGTTT TCGCTGGTCG 
CACCGCTGGC TAGTGGTCAA AGAGTCCTTC CTCTTGTACA TGAGCCGAGA CGACTCAGAA 
ATCGACTTTG TGCTGCTTTA TGACCCGGAG TTTGCCGTAA AAGTGGGCAA TGCTCATACA 
GGGACAGAGT ATGGAGTTTG CATTGAGAAC TACACACGGA GCTTAGTAAT CAAGTGCATC 
AGCTACAGAC AAAGTCAGTG GTGGAGCCAT GAAATTGACC GGCTGGCTCA CTTGTGTGAC 
TTCCTAAAAG TGAGCCGCTT TGGAGGATTC GCTCCACCAC GAGAAGATAC TCTTACTAAA 
TGGTATGTGA ACGGGAAAGA CTACTTTGCA GACCTGGCTG ATGCCCTTGA ACAAGCTAAG 
GAGGAGATTT TCATCACGGA TTGGTGGCTC AGTCCTGAAA TTTTTCTAAA GAGACCCGCC 
ACTGACAACC ACTGGAGACT GGACGAGATA CTTAAACGGA AAGCAGAACA AGGAGTCAAA 
GTATGTGTCC TGCTTTACAA GGAGGTGGAG ATGGCGCTTG GTATCAATAG TGACTACAGC 
AAGAGGACTC TGATGGACAT GCACCCAAAC ATCAAGGTGA TGCGACATCC AGACCACGTC 
TCATCTGCGG TGGTCTTGTG GGCTCACCAT GAAAAGATGG TGGCTATTGA CCAAACCGTG 
GCGTTTGTAG GGGGACTTGA CCTGGCGTTT GGCAGGTGGG ATGACAGAGA GTATCGGCTG 
GCTGACCTGG GTCAGGCAGA GTCGTTAAAC GGGGAAACGG CGGAGCATCC AAACACAGAT 
GCAACTGACA ATGGTGTGGG TGATGGCCAA AACGCAGGTA ACCAAGCAGA AAAGGACCAA 
CCTGATCTGA CTGGCAACAC TAAGCTTTGG CTGGGGAAAG ACTACAGCAA CTTCATCAAC 
AAAGACTGGA CCCTACTGGA CAAGCCGTTT GAAGATAACA TCGACCGCAC TAAAGTTCCT 
CGCATGCCTT GGCGGGACCT GTCTTCAGCT ATGCACGGCA AAGCGGCCAG GGATGTTGCC 
CGACACTTCA TCCAGCGCTG GAACTTCACA AAGATCTTCA AGACCAAGTA CAAGGACAAC 
TTCTACCCCT ACCTTCTCCC CAAGTCTAAC TCGACGGCAG ATTTGCTGCC ATTCACTATC 
CCTAGGTCCC AGAAAGCCAA AGTTCAGGTA CTGCGCTCTG CTGATCGGTG GTCAACTGGA 
ACAGCAGAGA ACTCAATCCT TAATGCCTAC ATACACACAA TTGAGAACAG CGAGCACTAC 
ATCTACATCG AGAACCAGTT TTTCATCAGC TGTGCCGACG ACAAGGTCGT CCAAAATAGA 
ATCGGGGATG CAATTGTGAA ACGAATCCTG CGAGCACACA GAGAGCAGAA GAAGTACAGG 
GTGTTTGTGG TGATTCCTTT ACTTCCTGGG TTTGAGGGAG ACATTACCAC GGGAGGTGGA 
AATGCAATTC AGGCGATTAT GCACTTCACT TACAGGACTA TGTGCCGTGG AGATCACTCA 
ATTCTGTCGA GGCTTAGTGA AGTAAAGGAC CAGTGGACTG AGTACATCAG CTTTTGTGGC 
CTCAGAACGC ATTCCCGTCT CTCTGGGTCG CTCGTCACAG AGCTGATCTA TGTCCACAGC 
AAGGCGCTCA TTGCTGATGA CCGCTGCTAT ATAATTGGAT CAGCCAACAT CAATGATCGC 
AGCATGCTGG GCACCCGAGA TAGTGAGCTG GCTGTGTTTG TGGAAGATGA AGAGAGGGTC 
CCATCCATCA TGGGAGGACA GGAATACGAG GCAGGACCGC TCGCGCTTGC TCTACGCAAA 
GAGTGCTTCA GTGTCCTGGT TGGAGCCGTA TCAGACCCCA GCATCAACAT TGACGATCCG 
ATCAGTGACG ACTTCTTCTT CCTGACCTGG AATGAAACGG CTAAACTCAA CACGGAGATC 
TACGCTAAGG TCTTCCGATG CCTCCCCTGC AACAGTGTTC ACAACATGCA GCGCCTGAGT 
GAGCACAACT GCGAAGAACG CCTCTGCGAC ACCGACCCCG AGCGGGCGGC GGAGGAGCTG 
AAGGCCGTCA GAGGGGTGCT GGTCCACTTT CCGCTGAAAT ACATGTGCGA GGAAAACCTG 
CTGCCGCCTC TGAACTGTAA AGAAGGCATG GCTCCTACAG ACCTTTGGAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLD2 ( XM_021318794.1 ) cDNA ORF clone, Fundulus heteroclitus(Mummichog) -> XP_021174469.1 Fundulus heteroclitus phospholipase D1 (LOC105929401), mRNA.

Price & Availability↑

CloneID	OFu138388
Clone ID Related Accession (Same CDS sequence)	XM_021318794.1
Accession Version	XM_021318794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	phospholipase D1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012234309.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGTC CAGAGGAAGT GATCGAGCCA GCGGTTGGGA GTTCAGCCGT ACCGCACCTC 
AGCAGGAGGC GCTTCTCGCA TGACATCAAT GATCTCAGTC CAGATGAATT AGACAGTCTC 
ATGAAAAACC GTGACGGCTG GGCTTTCACA GTAGTTCATC ATCTGCCTGA AATGAAGGAA 
GCTGGTGTAT CTTACTTCAT ACCGGGTACT CCTATCACAT GCAAGGTGGA CAACACAGAG 
AAACACACCA CTCGCTCAAA GGTGCGTGTG GGAACCCTGT ACACAGTGCG GTTAACTCAT 
GGACACTTCC ACTGGGTAGT GAAGCGGAAG TACAAGCACT TTCAGGAGCT TCACCGGGAC 
CTTTACAAAC ACAAGATGAT GCTTCACTAT CTGCCTCGAA GGTTCGCAAA GGACAGGCAG 
CAGCTGATGA TGACGTCAGA GGAAATGCCC AGCCTACACG GAACAGATCG CACCAGAAGA 
ACCTCAAGCA AAATGAAATA CCTTGAGGAG TACCTGAATG GGCTGCTGGA GAATGCATTC 
TGCAGATCTA TCCACAGCAT GCTTGAGTTC CTCTCTGTCA GTGCCCTCTC TTTTGTCAAT 
GATCTGGGAC CCAAAGGCCT GGAGGGGCCG ATCATGAAAA GGTCTGGCGG TCATCGGGTT 
CTGGGTCTGA ATTGCATCGG TCACCATCGG GTCTGTTTTC GCTGGTCGCA CCGCTGGCTA 
GTGGTCAAAG AGTCCTTCCT CTTGTACATG AGCCGAGACG ACTCAGAAAT CGACTTTGTG 
CTGCTTTATG ACCCGGAGTT TGCCGTAAAA GTGGGCAATG CTCATACAGG GACAGAGTAT 
GGAGTTTGCA TTGAGAACTA CACACGGAGC TTAGTAATCA AGTGCATCAG CTACAGACAA 
AGTCAGTGGT GGAGCCATGA AATTGACCGG CTGGCTCACT TGTGTGACTT CCTAAAAGTG 
AGCCGCTTTG GAGGATTCGC TCCACCACGA GAAGATACTC TTACTAAATG GTATGTGAAC 
GGGAAAGACT ACTTTGCAGA CCTGGCTGAT GCCCTTGAAC AAGCTAAGGA GGAGATTTTC 
ATCACGGATT GGTGGCTCAG TCCTGAAATT TTTCTAAAGA GACCCGCCAC TGACAACCAC 
TGGAGACTGG ACGAGATACT TAAACGGAAA GCAGAACAAG GAGTCAAAGT ATGTGTCCTG 
CTTTACAAGG AGGTGGAGAT GGCGCTTGGT ATCAATAGTG ACTACAGCAA GAGGACTCTG 
ATGGACATGC ACCCAAACAT CAAGGTGATG CGACATCCAG ACCACGTCTC ATCTGCGGTG 
GTCTTGTGGG CTCACCATGA AAAGATGGTG GCTATTGACC AAACCGTGGC GTTTGTAGGG 
GGACTTGACC TGGCGTTTGG CAGGTGGGAT GACAGAGAGT ATCGGCTGGC TGACCTGGGT 
CAGGCAGAGT CGTTAAACGG GGAAACGGCG GAGCATCCAA ACACAGATGC AACTGACAAT 
GGTGTGGGTG ATGGCCAAAA CGCAGGTAAC CAAGCAGAAA AGGACCAACC TGATCTGACT 
GGCAACACTA AGCTTTGGCT GGGGAAAGAC TACAGCAACT TCATCAACAA AGACTGGACC 
CTACTGGACA AGCCGTTTGA AGATAACATC GACCGCACTA AAGTTCCTCG CATGCCTTGG 
CGGGACCTGT CTTCAGCTAT GCACGGCAAA GCGGCCAGGG ATGTTGCCCG ACACTTCATC 
CAGCGCTGGA ACTTCACAAA GATCTTCAAG ACCAAGTACA AGGACAACTT CTACCCCTAC 
CTTCTCCCCA AGTCTAACTC GACGGCAGAT TTGCTGCCAT TCACTATCCC TAGGTCCCAG 
AAAGCCAAAG TTCAGGTACT GCGCTCTGCT GATCGGTGGT CAACTGGAAC AGCAGAGAAC 
TCAATCCTTA ATGCCTACAT ACACACAATT GAGAACAGCG AGCACTACAT CTACATCGAG 
AACCAGTTTT TCATCAGCTG TGCCGACGAC AAGGTCGTCC AAAATAGAAT CGGGGATGCA 
ATTGTGAAAC GAATCCTGCG AGCACACAGA GAGCAGAAGA AGTACAGGGT GTTTGTGGTG 
ATTCCTTTAC TTCCTGGGTT TGAGGGAGAC ATTACCACGG GAGGTGGAAA TGCAATTCAG 
GCGATTATGC ACTTCACTTA CAGGACTATG TGCCGTGGAG ATCACTCAAT TCTGTCGAGG 
CTTAGTGAAG TAAAGGACCA GTGGACTGAG TACATCAGCT TTTGTGGCCT CAGAACGCAT 
TCCCGTCTCT CTGGGTCGCT CGTCACAGAG CTGATCTATG TCCACAGCAA GGCGCTCATT 
GCTGATGACC GCTGCTATAT AATTGGATCA GCCAACATCA ATGATCGCAG CATGCTGGGC 
ACCCGAGATA GTGAGCTGGC TGTGTTTGTG GAAGATGAAG AGAGGGTCCC ATCCATCATG 
GGAGGACAGG AATACGAGGC AGGACCGCTC GCGCTTGCTC TACGCAAAGA GTGCTTCAGT 
GTCCTGGTTG GAGCCGTATC AGACCCCAGC ATCAACATTG ACGATCCGAT CAGTGACGAC 
TTCTTCTTCC TGACCTGGAA TGAAACGGCT AAACTCAACA CGGAGATCTA CGCTAAGGTC 
TTCCGATGCC TCCCCTGCAA CAGTGTTCAC AACATGCAGC GCCTGAGTGA GCACAACTGC 
GAAGAACGCC TCTGCGACAC CGACCCCGAG CGGGCGGCGG AGGAGCTGAA GGCCGTCAGA 
GGGGTGCTGG TCCACTTTCC GCTGAAATAC ATGTGCGAGG AAAACCTGCT GCCGCCTCTG 
AACTGTAAAG AAGGCATGGC TCCTACAGAC CTTTGGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021174469.1
CDS	204..3065
Translation	MASPEEVIEPAVGSSAVPHLSRRRFSHDINDLSPDELDSLMKNRDGWAFTVVHHLPEMKEAGVSYFIPGTPITCKVDNTEKHTTRSKVRVGTLYTVRLTHGHFHWVVKRKYKHFQELHRDLYKHKMMLHYLPRRFAKDRQQLMMTSEEMPSLHGTDRTRRTSSKMKYLEEYLNGLLENAFCRSIHSMLEFLSVSALSFVNDLGPKGLEGPIMKRSGGHRVLGLNCIGHHRVCFRWSHRWLVVKESFLLYMSRDDSEIDFVLLYDPEFAVKVGNAHTGTEYGVCIENYTRSLVIKCISYRQSQWWSHEIDRLAHLCDFLKVSRFGGFAPPREDTLTKWYVNGKDYFADLADALEQAKEEIFITDWWLSPEIFLKRPATDNHWRLDEILKRKAEQGVKVCVLLYKEVEMALGINSDYSKRTLMDMHPNIKVMRHPDHVSSAVVLWAHHEKMVAIDQTVAFVGGLDLAFGRWDDREYRLADLGQAESLNGETAEHPNTDATDNGVGDGQNAGNQAEKDQPDLTGNTKLWLGKDYSNFINKDWTLLDKPFEDNIDRTKVPRMPWRDLSSAMHGKAARDVARHFIQRWNFTKIFKTKYKDNFYPYLLPKSNSTADLLPFTIPRSQKAKVQVLRSADRWSTGTAENSILNAYIHTIENSEHYIYIENQFFISCADDKVVQNRIGDAIVKRILRAHREQKKYRVFVVIPLLPGFEGDITTGGGNAIQAIMHFTYRTMCRGDHSILSRLSEVKDQWTEYISFCGLRTHSRLSGSLVTELIYVHSKALIADDRCYIIGSANINDRSMLGTRDSELAVFVEDEERVPSIMGGQEYEAGPLALALRKECFSVLVGAVSDPSINIDDPISDDFFFLTWNETAKLNTEIYAKVFRCLPCNSVHNMQRLSEHNCEERLCDTDPERAAEELKAVRGVLVHFPLKYMCEENLLPPLNCKEGMAPTDLWT

Target ORF information:

RefSeq Version	XM_021318794.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus phospholipase D1 (LOC105929401), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021318794.1

ORF Insert Sequence:

ATGGCCAGTC CAGAGGAAGT GATCGAGCCA GCGGTTGGGA GTTCAGCCGT ACCGCACCTC 
AGCAGGAGGC GCTTCTCGCA TGACATCAAT GATCTCAGTC CAGATGAATT AGACAGTCTC 
ATGAAAAACC GTGACGGCTG GGCTTTCACA GTAGTTCATC ATCTGCCTGA AATGAAGGAA 
GCTGGTGTAT CTTACTTCAT ACCGGGTACT CCTATCACAT GCAAGGTGGA CAACACAGAG 
AAACACACCA CTCGCTCAAA GGTGCGTGTG GGAACCCTGT ACACAGTGCG GTTAACTCAT 
GGACACTTCC ACTGGGTAGT GAAGCGGAAG TACAAGCACT TTCAGGAGCT TCACCGGGAC 
CTTTACAAAC ACAAGATGAT GCTTCACTAT CTGCCTCGAA GGTTCGCAAA GGACAGGCAG 
CAGCTGATGA TGACGTCAGA GGAAATGCCC AGCCTACACG GAACAGATCG CACCAGAAGA 
ACCTCAAGCA AAATGAAATA CCTTGAGGAG TACCTGAATG GGCTGCTGGA GAATGCATTC 
TGCAGATCTA TCCACAGCAT GCTTGAGTTC CTCTCTGTCA GTGCCCTCTC TTTTGTCAAT 
GATCTGGGAC CCAAAGGCCT GGAGGGGCCG ATCATGAAAA GGTCTGGCGG TCATCGGGTT 
CTGGGTCTGA ATTGCATCGG TCACCATCGG GTCTGTTTTC GCTGGTCGCA CCGCTGGCTA 
GTGGTCAAAG AGTCCTTCCT CTTGTACATG AGCCGAGACG ACTCAGAAAT CGACTTTGTG 
CTGCTTTATG ACCCGGAGTT TGCCGTAAAA GTGGGCAATG CTCATACAGG GACAGAGTAT 
GGAGTTTGCA TTGAGAACTA CACACGGAGC TTAGTAATCA AGTGCATCAG CTACAGACAA 
AGTCAGTGGT GGAGCCATGA AATTGACCGG CTGGCTCACT TGTGTGACTT CCTAAAAGTG 
AGCCGCTTTG GAGGATTCGC TCCACCACGA GAAGATACTC TTACTAAATG GTATGTGAAC 
GGGAAAGACT ACTTTGCAGA CCTGGCTGAT GCCCTTGAAC AAGCTAAGGA GGAGATTTTC 
ATCACGGATT GGTGGCTCAG TCCTGAAATT TTTCTAAAGA GACCCGCCAC TGACAACCAC 
TGGAGACTGG ACGAGATACT TAAACGGAAA GCAGAACAAG GAGTCAAAGT ATGTGTCCTG 
CTTTACAAGG AGGTGGAGAT GGCGCTTGGT ATCAATAGTG ACTACAGCAA GAGGACTCTG 
ATGGACATGC ACCCAAACAT CAAGGTGATG CGACATCCAG ACCACGTCTC ATCTGCGGTG 
GTCTTGTGGG CTCACCATGA AAAGATGGTG GCTATTGACC AAACCGTGGC GTTTGTAGGG 
GGACTTGACC TGGCGTTTGG CAGGTGGGAT GACAGAGAGT ATCGGCTGGC TGACCTGGGT 
CAGGCAGAGT CGTTAAACGG GGAAACGGCG GAGCATCCAA ACACAGATGC AACTGACAAT 
GGTGTGGGTG ATGGCCAAAA CGCAGGTAAC CAAGCAGAAA AGGACCAACC TGATCTGACT 
GGCAACACTA AGCTTTGGCT GGGGAAAGAC TACAGCAACT TCATCAACAA AGACTGGACC 
CTACTGGACA AGCCGTTTGA AGATAACATC GACCGCACTA AAGTTCCTCG CATGCCTTGG 
CGGGACCTGT CTTCAGCTAT GCACGGCAAA GCGGCCAGGG ATGTTGCCCG ACACTTCATC 
CAGCGCTGGA ACTTCACAAA GATCTTCAAG ACCAAGTACA AGGACAACTT CTACCCCTAC 
CTTCTCCCCA AGTCTAACTC GACGGCAGAT TTGCTGCCAT TCACTATCCC TAGGTCCCAG 
AAAGCCAAAG TTCAGGTACT GCGCTCTGCT GATCGGTGGT CAACTGGAAC AGCAGAGAAC 
TCAATCCTTA ATGCCTACAT ACACACAATT GAGAACAGCG AGCACTACAT CTACATCGAG 
AACCAGTTTT TCATCAGCTG TGCCGACGAC AAGGTCGTCC AAAATAGAAT CGGGGATGCA 
ATTGTGAAAC GAATCCTGCG AGCACACAGA GAGCAGAAGA AGTACAGGGT GTTTGTGGTG 
ATTCCTTTAC TTCCTGGGTT TGAGGGAGAC ATTACCACGG GAGGTGGAAA TGCAATTCAG 
GCGATTATGC ACTTCACTTA CAGGACTATG TGCCGTGGAG ATCACTCAAT TCTGTCGAGG 
CTTAGTGAAG TAAAGGACCA GTGGACTGAG TACATCAGCT TTTGTGGCCT CAGAACGCAT 
TCCCGTCTCT CTGGGTCGCT CGTCACAGAG CTGATCTATG TCCACAGCAA GGCGCTCATT 
GCTGATGACC GCTGCTATAT AATTGGATCA GCCAACATCA ATGATCGCAG CATGCTGGGC 
ACCCGAGATA GTGAGCTGGC TGTGTTTGTG GAAGATGAAG AGAGGGTCCC ATCCATCATG 
GGAGGACAGG AATACGAGGC AGGACCGCTC GCGCTTGCTC TACGCAAAGA GTGCTTCAGT 
GTCCTGGTTG GAGCCGTATC AGACCCCAGC ATCAACATTG ACGATCCGAT CAGTGACGAC 
TTCTTCTTCC TGACCTGGAA TGAAACGGCT AAACTCAACA CGGAGATCTA CGCTAAGGTC 
TTCCGATGCC TCCCCTGCAA CAGTGTTCAC AACATGCAGC GCCTGAGTGA GCACAACTGC 
GAAGAACGCC TCTGCGACAC CGACCCCGAG CGGGCGGCGG AGGAGCTGAA GGCCGTCAGA 
GGGGTGCTGG TCCACTTTCC GCTGAAATAC ATGTGCGAGG AAAACCTGCT GCCGCCTCTG 
AACTGTAAAG AAGGCATGGC TCCTACAGAC CTTTGGACAT AA