LOC105920143 cDNA ORF clone, Fundulus heteroclitus(mummichog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105920143
Entrez Gene ID	105920143
Full Name	E3 ubiquitin-protein ligase BRE1A
Synonyms	rnf20
Gene Type	protein-coding
Organism	Fundulus heteroclitus(mummichog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012855652.1	XP_012711106.1	E3 ubiquitin-protein ligase BRE1A
XM_012855652.2	XP_012711106.1	E3 ubiquitin-protein ligase BRE1A

Pathways from BioSystems
Gene Ontology

The following LOC105920143 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105920143 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFu104603	XM_012855652.2 Latest version!	Fundulus heteroclitus E3 ubiquitin-protein ligase BRE1A (LOC105920143), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OFu104603	XM_012855652.1	Fundulus heteroclitus ring finger protein 20, E3 ubiquitin protein ligase (rnf20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105920143 ( XM_012855652.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012711106.1 Fundulus heteroclitus ring finger protein 20, E3 ubiquitin protein ligase (rnf20), mRNA.

Price & Availability↑

CloneID	OFu104603
Clone ID Related Accession (Same CDS sequence)	XM_012855652.1 , XM_012855652.2
Accession Version	XM_012855652.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	E3 ubiquitin-protein ligase BRE1A
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGC AGAAGCGTCC AGCTGACCCC AGCGGGCCCT CTTCCTCCGG TGCCCCCGCA 
GAGAAGCGGA GGGAGCGGGA TGGAGAGGAC GGAGGTCCCG GTGTGAGCGC CGGAGCCGGA 
GGGAGCACCG CGGTGGAGAC GGTCATCAAA CTGGGAGGAG TGTCCAACTC GGAGGAACAA 
GACATAAAAG CTCTCCAAGC TAAGAACCGA AAGTTGGGAG AATCTCTCGA TCAAAGACAG 
GTGATCGAGG ACGAGCTGCG AGAGCGAATT GAGAGACTGG AGACCCGACA GGCCACGGAC 
GACGCCAGTT TGCTGATTCT GAACAGATAC TGGAACCAGT TTGATGAGAA CATCCGGCTC 
CTCATCCGGC GCTATGACCA AGCGAGCAGC GAACCGGAGA AATCTCCAAC AGGCGAGGGA 
CGCAGCCTGA AGCCGGAAAC TCCAGAACCC GATGGAGATT CGAACCAGGA GCGGGCCAAA 
GACAGAGGAC ATCAAGGGGA GACGACAAAC TCCTTTTTGG CCACGCTGGC GAGCAGCACC 
AGCGAGGAGA TGGAGGCCGA GCTCCAGGAG AGGGTGGAGT CCAGTCAGAA GCAGGCCAAT 
CGTGTGGTGG AGATCTACAA GAACCTGAAG AGCACCTTGG ACCAGCTGAA AGCAGAGCTT 
GACTCAGGGA CTGAGGGTGG TCTGAAGCAG GTCGCAGCCA AGCTAAACTC CCTCCTGAGC 
CGGGAAAACG AGCTGCTTCA ACAGCTGACC GAGGACCTCA AACAGAAACA CAGCCACATG 
ACCAACGAGT CCCGGTCGTT GGGCCGGGCG GCTAACAAAG CGGACCAGCG CGTCAGCGAG 
CTGCAGGCTC GGATCGAGGA GCTCCAGTGG GACATGGAGA AGATCCGCCG TCGAGAGAAC 
AGGCTGAATG CCCACCTGAG TGAAATACTG GAGAGGGTGA ACAGCAAAGG CTACAAAGTG 
TGTGGCGAGG CCAGCAGTGT GTGTGGAACC ATCACCATTA ACAAGAGAAA GTTTGAGGAG 
ATGAACAGCG AGTTGGAGGA GAACACGGAA CTGGCAGAAA ATCGGCTGGT TGAGCTGCAG 
AAGCTCCAGC AGGACCTGCA AAACGTCCAG CAGGAGAACA ACAGCATGAA GACGGAGCTC 
GTGAGTCGAG CTGAGGGCCT GGTCAAAGAA TCCTCAGAGT ATCGCTGCCT CCAGTCCCAG 
TTTTCCGTGC TCTACAACGA GTCCCTGACC CTCAAGGCCC AGCTAGACGA AACCCGGGCT 
CGCCTCAACA CTACCCGCAC GGCTCGCCTC AGACAGCTGG AGCACATGGA GAACGACGAG 
GTGGCTCTGC AGAGGAAGGT CCGCACCGAA GTGTTTCAGC TGGAGGACAC GTTGGCTCAG 
GTCAAGAAGG AGTACGAGAT GTTGCGCATT GAATTTGAAC AGACTCTTGC CGCCAACGAG 
CAAGCAGGTC CCATCAACAG GGAGATGCGT CACCTTATAA GCACGCTGCA GACACACAAC 
CAGCAGCTGA AAGGAGAGGT GGTGAAGTAC AAGCTGAGAC TGAGAGAGAC CCAAGCTGAG 
CTCCACCAGA TCCGTGCCTC GAAGGGCAGC GCCGTCCTCC AGTCGCAGTC GAGCACGGAG 
ATGGACGTGA AGGACGAGAT GACCTCTCCT TCGACCCCGG CCGTTTCTGG AGAACCCGCG 
GTCAAAGCGG AGCCGGACAA CGGCTCCTCT ACACCCAGCA GCACCGGGAT TTCAGTGAAA 
ACAGAACCTG GAACTGAACC GGACGCAAGC ATAAAAGAGG AGGAAAAAGA GGAGAGAAAA 
GAGAAGGAAG AGAAGAAAGA GAAAGACTTT ATAAAGAAAG AGGAGAAAGA CAGGGAGAGA 
GAACGGGAGA AGGAGAAGGA GAGAGAGAGG CCCACCCGGA GCAGCAGCAG CAGCAGCGGG 
ATGAAGGAGG AGAGGGAGAA GCCGGGCGTC AGCAGCCAGC AGGAGGAGAC GGCCGGGGAT 
CGGCTCGCGA TGCTCGGAGG CTCAAAGAGG AAAGAGATGG AGCAGCTGAA AATCGTCCGA 
GCAGAACTAA AGAAAGCCCA AGAGTCCCAG AGAGAGATGA AGCTTCTGCT GGACATGTAC 
CGATCAGCAC CAAAGGAGCA GAGGGACAAA GTCCAGCTTA TGGCTGCAGA GAAAAAGACC 
AAGTCAGAGG CAGAGGAGCT GCGGCAGAGG CTGCGGGAGC TGGAGGAGAG GGAGAGAAGG 
GAGGGCAAGA AAATGGCGGA CGAAGAAGCT CTGAGGAAGA TCCGCTCAGT CGAAGAACAG 
ATCGACATCC TCAACAAGAA GCTGTCCATC GCCAAACAGG AGGAAGACGC GCTGCTGAGC 
GAGATGGACG TGACGGGCCA GGCCTTCGAG GACATGCAGG AACAGAATAT CCGCCTCATG 
CAGCAGCTCC GAGAAAAGGA CGATGCCAAC TTCAAGCTGA TGAGCGAGCG GATCAAGTCC 
AACCAGATCC ACAAGCTCCT GAAGGAGGAA AAGGAAGAGC TAGCCGACCA ACTGCTAACT 
TTAAAAACAC AGGTAGATGC ACAGCTGCAG GTAGTCAGGA AGCTGGAGGA GAAGGAGCGC 
CTCCTGCAGG GGACTATTAG CACTGCGGAG AGAGAGCTGG GACTTCGAAC ACAAGCTTTG 
GACATGAACA AGCGAAAGGC TCAGGAGTCG GCGCTGCTGT CGGAGGAGCT GCGCGCTCAG 
CTGGAGCAGG TCCAGCAGAA GCTGAAGCTG GTCAGAGAGG AGGTCGTTGA GAACAGCATC 
TCCAGAGAGA AGGAGTCTTT CAATGCCCGG CGGGCGCAGG AGGACATCTC CAAGCTCAGG 
AGAAAGATTG AGAAGGCCAA GAAACCGGCT GAGAAGATAA GCAACGGAGA TGAGATCCTG 
AACGAAGAAA TAAATGAGTA CAAGGCTCGG CTGACGTGCC CGTGCTGCAA CTCCCGGGTG 
AAGGATGCCG TCCTGACAAA ATGCTTCCAC GTCTTCTGCT TCGAGTGCGT CAAGACGCGC 
TACGACACCC GGCAGAGGAA GTGCCCCAAG TGCAATGCCG CCTTCGGGGC GAACGACTTC 
CATCGCATTT ACATCGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012711106.1
CDS	131..3211
Translation	MSGQKRPADPSGPSSSGAPAEKRRERDGEDGGPGVSAGAGGSTAVETVIKLGGVSNSEEQDIKALQAKNRKLGESLDQRQVIEDELRERIERLETRQATDDASLLILNRYWNQFDENIRLLIRRYDQASSEPEKSPTGEGRSLKPETPEPDGDSNQERAKDRGHQGETTNSFLATLASSTSEEMEAELQERVESSQKQANRVVEIYKNLKSTLDQLKAELDSGTEGGLKQVAAKLNSLLSRENELLQQLTEDLKQKHSHMTNESRSLGRAANKADQRVSELQARIEELQWDMEKIRRRENRLNAHLSEILERVNSKGYKVCGEASSVCGTITINKRKFEEMNSELEENTELAENRLVELQKLQQDLQNVQQENNSMKTELVSRAEGLVKESSEYRCLQSQFSVLYNESLTLKAQLDETRARLNTTRTARLRQLEHMENDEVALQRKVRTEVFQLEDTLAQVKKEYEMLRIEFEQTLAANEQAGPINREMRHLISTLQTHNQQLKGEVVKYKLRLRETQAELHQIRASKGSAVLQSQSSTEMDVKDEMTSPSTPAVSGEPAVKAEPDNGSSTPSSTGISVKTEPGTEPDASIKEEEKEERKEKEEKKEKDFIKKEEKDREREREKEKERERPTRSSSSSSGMKEEREKPGVSSQQEETAGDRLAMLGGSKRKEMEQLKIVRAELKKAQESQREMKLLLDMYRSAPKEQRDKVQLMAAEKKTKSEAEELRQRLRELEERERREGKKMADEEALRKIRSVEEQIDILNKKLSIAKQEEDALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQLLTLKTQVDAQLQVVRKLEEKERLLQGTISTAERELGLRTQALDMNKRKAQESALLSEELRAQLEQVQQKLKLVREEVVENSISREKESFNARRAQEDISKLRRKIEKAKKPAEKISNGDEILNEEINEYKARLTCPCCNSRVKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	XM_012855652.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus ring finger protein 20, E3 ubiquitin protein ligase (rnf20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012855652.1

ORF Insert Sequence:

ATGTCGGGGC AGAAGCGTCC AGCTGACCCC AGCGGGCCCT CTTCCTCCGG TGCCCCCGCA 
GAGAAGCGGA GGGAGCGGGA TGGAGAGGAC GGAGGTCCCG GTGTGAGCGC CGGAGCCGGA 
GGGAGCACCG CGGTGGAGAC GGTCATCAAA CTGGGAGGAG TGTCCAACTC GGAGGAACAA 
GACATAAAAG CTCTCCAAGC TAAGAACCGA AAGTTGGGAG AATCTCTCGA TCAAAGACAG 
GTGATCGAGG ACGAGCTGCG AGAGCGAATT GAGAGACTGG AGACCCGACA GGCCACGGAC 
GACGCCAGTT TGCTGATTCT GAACAGATAC TGGAACCAGT TTGATGAGAA CATCCGGCTC 
CTCATCCGGC GCTATGACCA AGCGAGCAGC GAACCGGAGA AATCTCCAAC AGGCGAGGGA 
CGCAGCCTGA AGCCGGAAAC TCCAGAACCC GATGGAGATT CGAACCAGGA GCGGGCCAAA 
GACAGAGGAC ATCAAGGGGA GACGACAAAC TCCTTTTTGG CCACGCTGGC GAGCAGCACC 
AGCGAGGAGA TGGAGGCCGA GCTCCAGGAG AGGGTGGAGT CCAGTCAGAA GCAGGCCAAT 
CGTGTGGTGG AGATCTACAA GAACCTGAAG AGCACCTTGG ACCAGCTGAA AGCAGAGCTT 
GACTCAGGGA CTGAGGGTGG TCTGAAGCAG GTCGCAGCCA AGCTAAACTC CCTCCTGAGC 
CGGGAAAACG AGCTGCTTCA ACAGCTGACC GAGGACCTCA AACAGAAACA CAGCCACATG 
ACCAACGAGT CCCGGTCGTT GGGCCGGGCG GCTAACAAAG CGGACCAGCG CGTCAGCGAG 
CTGCAGGCTC GGATCGAGGA GCTCCAGTGG GACATGGAGA AGATCCGCCG TCGAGAGAAC 
AGGCTGAATG CCCACCTGAG TGAAATACTG GAGAGGGTGA ACAGCAAAGG CTACAAAGTG 
TGTGGCGAGG CCAGCAGTGT GTGTGGAACC ATCACCATTA ACAAGAGAAA GTTTGAGGAG 
ATGAACAGCG AGTTGGAGGA GAACACGGAA CTGGCAGAAA ATCGGCTGGT TGAGCTGCAG 
AAGCTCCAGC AGGACCTGCA AAACGTCCAG CAGGAGAACA ACAGCATGAA GACGGAGCTC 
GTGAGTCGAG CTGAGGGCCT GGTCAAAGAA TCCTCAGAGT ATCGCTGCCT CCAGTCCCAG 
TTTTCCGTGC TCTACAACGA GTCCCTGACC CTCAAGGCCC AGCTAGACGA AACCCGGGCT 
CGCCTCAACA CTACCCGCAC GGCTCGCCTC AGACAGCTGG AGCACATGGA GAACGACGAG 
GTGGCTCTGC AGAGGAAGGT CCGCACCGAA GTGTTTCAGC TGGAGGACAC GTTGGCTCAG 
GTCAAGAAGG AGTACGAGAT GTTGCGCATT GAATTTGAAC AGACTCTTGC CGCCAACGAG 
CAAGCAGGTC CCATCAACAG GGAGATGCGT CACCTTATAA GCACGCTGCA GACACACAAC 
CAGCAGCTGA AAGGAGAGGT GGTGAAGTAC AAGCTGAGAC TGAGAGAGAC CCAAGCTGAG 
CTCCACCAGA TCCGTGCCTC GAAGGGCAGC GCCGTCCTCC AGTCGCAGTC GAGCACGGAG 
ATGGACGTGA AGGACGAGAT GACCTCTCCT TCGACCCCGG CCGTTTCTGG AGAACCCGCG 
GTCAAAGCGG AGCCGGACAA CGGCTCCTCT ACACCCAGCA GCACCGGGAT TTCAGTGAAA 
ACAGAACCTG GAACTGAACC GGACGCAAGC ATAAAAGAGG AGGAAAAAGA GGAGAGAAAA 
GAGAAGGAAG AGAAGAAAGA GAAAGACTTT ATAAAGAAAG AGGAGAAAGA CAGGGAGAGA 
GAACGGGAGA AGGAGAAGGA GAGAGAGAGG CCCACCCGGA GCAGCAGCAG CAGCAGCGGG 
ATGAAGGAGG AGAGGGAGAA GCCGGGCGTC AGCAGCCAGC AGGAGGAGAC GGCCGGGGAT 
CGGCTCGCGA TGCTCGGAGG CTCAAAGAGG AAAGAGATGG AGCAGCTGAA AATCGTCCGA 
GCAGAACTAA AGAAAGCCCA AGAGTCCCAG AGAGAGATGA AGCTTCTGCT GGACATGTAC 
CGATCAGCAC CAAAGGAGCA GAGGGACAAA GTCCAGCTTA TGGCTGCAGA GAAAAAGACC 
AAGTCAGAGG CAGAGGAGCT GCGGCAGAGG CTGCGGGAGC TGGAGGAGAG GGAGAGAAGG 
GAGGGCAAGA AAATGGCGGA CGAAGAAGCT CTGAGGAAGA TCCGCTCAGT CGAAGAACAG 
ATCGACATCC TCAACAAGAA GCTGTCCATC GCCAAACAGG AGGAAGACGC GCTGCTGAGC 
GAGATGGACG TGACGGGCCA GGCCTTCGAG GACATGCAGG AACAGAATAT CCGCCTCATG 
CAGCAGCTCC GAGAAAAGGA CGATGCCAAC TTCAAGCTGA TGAGCGAGCG GATCAAGTCC 
AACCAGATCC ACAAGCTCCT GAAGGAGGAA AAGGAAGAGC TAGCCGACCA ACTGCTAACT 
TTAAAAACAC AGGTAGATGC ACAGCTGCAG GTAGTCAGGA AGCTGGAGGA GAAGGAGCGC 
CTCCTGCAGG GGACTATTAG CACTGCGGAG AGAGAGCTGG GACTTCGAAC ACAAGCTTTG 
GACATGAACA AGCGAAAGGC TCAGGAGTCG GCGCTGCTGT CGGAGGAGCT GCGCGCTCAG 
CTGGAGCAGG TCCAGCAGAA GCTGAAGCTG GTCAGAGAGG AGGTCGTTGA GAACAGCATC 
TCCAGAGAGA AGGAGTCTTT CAATGCCCGG CGGGCGCAGG AGGACATCTC CAAGCTCAGG 
AGAAAGATTG AGAAGGCCAA GAAACCGGCT GAGAAGATAA GCAACGGAGA TGAGATCCTG 
AACGAAGAAA TAAATGAGTA CAAGGCTCGG CTGACGTGCC CGTGCTGCAA CTCCCGGGTG 
AAGGATGCCG TCCTGACAAA ATGCTTCCAC GTCTTCTGCT TCGAGTGCGT CAAGACGCGC 
TACGACACCC GGCAGAGGAA GTGCCCCAAG TGCAATGCCG CCTTCGGGGC GAACGACTTC 
CATCGCATTT ACATCGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105920143 ( XM_012855652.2 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012711106.1 Fundulus heteroclitus E3 ubiquitin-protein ligase BRE1A (LOC105920143), mRNA.

Price & Availability↑

CloneID	OFu104603
Clone ID Related Accession (Same CDS sequence)	XM_012855652.1 , XM_012855652.2
Accession Version	XM_012855652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	E3 ubiquitin-protein ligase BRE1A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 1, 2017 this sequence version replaced XM_012855652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGC AGAAGCGTCC AGCTGACCCC AGCGGGCCCT CTTCCTCCGG TGCCCCCGCA 
GAGAAGCGGA GGGAGCGGGA TGGAGAGGAC GGAGGTCCCG GTGTGAGCGC CGGAGCCGGA 
GGGAGCACCG CGGTGGAGAC GGTCATCAAA CTGGGAGGAG TGTCCAACTC GGAGGAACAA 
GACATAAAAG CTCTCCAAGC TAAGAACCGA AAGTTGGGAG AATCTCTCGA TCAAAGACAG 
GTGATCGAGG ACGAGCTGCG AGAGCGAATT GAGAGACTGG AGACCCGACA GGCCACGGAC 
GACGCCAGTT TGCTGATTCT GAACAGATAC TGGAACCAGT TTGATGAGAA CATCCGGCTC 
CTCATCCGGC GCTATGACCA AGCGAGCAGC GAACCGGAGA AATCTCCAAC AGGCGAGGGA 
CGCAGCCTGA AGCCGGAAAC TCCAGAACCC GATGGAGATT CGAACCAGGA GCGGGCCAAA 
GACAGAGGAC ATCAAGGGGA GACGACAAAC TCCTTTTTGG CCACGCTGGC GAGCAGCACC 
AGCGAGGAGA TGGAGGCCGA GCTCCAGGAG AGGGTGGAGT CCAGTCAGAA GCAGGCCAAT 
CGTGTGGTGG AGATCTACAA GAACCTGAAG AGCACCTTGG ACCAGCTGAA AGCAGAGCTT 
GACTCAGGGA CTGAGGGTGG TCTGAAGCAG GTCGCAGCCA AGCTAAACTC CCTCCTGAGC 
CGGGAAAACG AGCTGCTTCA ACAGCTGACC GAGGACCTCA AACAGAAACA CAGCCACATG 
ACCAACGAGT CCCGGTCGTT GGGCCGGGCG GCTAACAAAG CGGACCAGCG CGTCAGCGAG 
CTGCAGGCTC GGATCGAGGA GCTCCAGTGG GACATGGAGA AGATCCGCCG TCGAGAGAAC 
AGGCTGAATG CCCACCTGAG TGAAATACTG GAGAGGGTGA ACAGCAAAGG CTACAAAGTG 
TGTGGCGAGG CCAGCAGTGT GTGTGGAACC ATCACCATTA ACAAGAGAAA GTTTGAGGAG 
ATGAACAGCG AGTTGGAGGA GAACACGGAA CTGGCAGAAA ATCGGCTGGT TGAGCTGCAG 
AAGCTCCAGC AGGACCTGCA AAACGTCCAG CAGGAGAACA ACAGCATGAA GACGGAGCTC 
GTGAGTCGAG CTGAGGGCCT GGTCAAAGAA TCCTCAGAGT ATCGCTGCCT CCAGTCCCAG 
TTTTCCGTGC TCTACAACGA GTCCCTGACC CTCAAGGCCC AGCTAGACGA AACCCGGGCT 
CGCCTCAACA CTACCCGCAC GGCTCGCCTC AGACAGCTGG AGCACATGGA GAACGACGAG 
GTGGCTCTGC AGAGGAAGGT CCGCACCGAA GTGTTTCAGC TGGAGGACAC GTTGGCTCAG 
GTCAAGAAGG AGTACGAGAT GTTGCGCATT GAATTTGAAC AGACTCTTGC CGCCAACGAG 
CAAGCAGGTC CCATCAACAG GGAGATGCGT CACCTTATAA GCACGCTGCA GACACACAAC 
CAGCAGCTGA AAGGAGAGGT GGTGAAGTAC AAGCTGAGAC TGAGAGAGAC CCAAGCTGAG 
CTCCACCAGA TCCGTGCCTC GAAGGGCAGC GCCGTCCTCC AGTCGCAGTC GAGCACGGAG 
ATGGACGTGA AGGACGAGAT GACCTCTCCT TCGACCCCGG CCGTTTCTGG AGAACCCGCG 
GTCAAAGCGG AGCCGGACAA CGGCTCCTCT ACACCCAGCA GCACCGGGAT TTCAGTGAAA 
ACAGAACCTG GAACTGAACC GGACGCAAGC ATAAAAGAGG AGGAAAAAGA GGAGAGAAAA 
GAGAAGGAAG AGAAGAAAGA GAAAGACTTT ATAAAGAAAG AGGAGAAAGA CAGGGAGAGA 
GAACGGGAGA AGGAGAAGGA GAGAGAGAGG CCCACCCGGA GCAGCAGCAG CAGCAGCGGG 
ATGAAGGAGG AGAGGGAGAA GCCGGGCGTC AGCAGCCAGC AGGAGGAGAC GGCCGGGGAT 
CGGCTCGCGA TGCTCGGAGG CTCAAAGAGG AAAGAGATGG AGCAGCTGAA AATCGTCCGA 
GCAGAACTAA AGAAAGCCCA AGAGTCCCAG AGAGAGATGA AGCTTCTGCT GGACATGTAC 
CGATCAGCAC CAAAGGAGCA GAGGGACAAA GTCCAGCTTA TGGCTGCAGA GAAAAAGACC 
AAGTCAGAGG CAGAGGAGCT GCGGCAGAGG CTGCGGGAGC TGGAGGAGAG GGAGAGAAGG 
GAGGGCAAGA AAATGGCGGA CGAAGAAGCT CTGAGGAAGA TCCGCTCAGT CGAAGAACAG 
ATCGACATCC TCAACAAGAA GCTGTCCATC GCCAAACAGG AGGAAGACGC GCTGCTGAGC 
GAGATGGACG TGACGGGCCA GGCCTTCGAG GACATGCAGG AACAGAATAT CCGCCTCATG 
CAGCAGCTCC GAGAAAAGGA CGATGCCAAC TTCAAGCTGA TGAGCGAGCG GATCAAGTCC 
AACCAGATCC ACAAGCTCCT GAAGGAGGAA AAGGAAGAGC TAGCCGACCA ACTGCTAACT 
TTAAAAACAC AGGTAGATGC ACAGCTGCAG GTAGTCAGGA AGCTGGAGGA GAAGGAGCGC 
CTCCTGCAGG GGACTATTAG CACTGCGGAG AGAGAGCTGG GACTTCGAAC ACAAGCTTTG 
GACATGAACA AGCGAAAGGC TCAGGAGTCG GCGCTGCTGT CGGAGGAGCT GCGCGCTCAG 
CTGGAGCAGG TCCAGCAGAA GCTGAAGCTG GTCAGAGAGG AGGTCGTTGA GAACAGCATC 
TCCAGAGAGA AGGAGTCTTT CAATGCCCGG CGGGCGCAGG AGGACATCTC CAAGCTCAGG 
AGAAAGATTG AGAAGGCCAA GAAACCGGCT GAGAAGATAA GCAACGGAGA TGAGATCCTG 
AACGAAGAAA TAAATGAGTA CAAGGCTCGG CTGACGTGCC CGTGCTGCAA CTCCCGGGTG 
AAGGATGCCG TCCTGACAAA ATGCTTCCAC GTCTTCTGCT TCGAGTGCGT CAAGACGCGC 
TACGACACCC GGCAGAGGAA GTGCCCCAAG TGCAATGCCG CCTTCGGGGC GAACGACTTC 
CATCGCATTT ACATCGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012711106.1
CDS	102..3182
Translation	MSGQKRPADPSGPSSSGAPAEKRRERDGEDGGPGVSAGAGGSTAVETVIKLGGVSNSEEQDIKALQAKNRKLGESLDQRQVIEDELRERIERLETRQATDDASLLILNRYWNQFDENIRLLIRRYDQASSEPEKSPTGEGRSLKPETPEPDGDSNQERAKDRGHQGETTNSFLATLASSTSEEMEAELQERVESSQKQANRVVEIYKNLKSTLDQLKAELDSGTEGGLKQVAAKLNSLLSRENELLQQLTEDLKQKHSHMTNESRSLGRAANKADQRVSELQARIEELQWDMEKIRRRENRLNAHLSEILERVNSKGYKVCGEASSVCGTITINKRKFEEMNSELEENTELAENRLVELQKLQQDLQNVQQENNSMKTELVSRAEGLVKESSEYRCLQSQFSVLYNESLTLKAQLDETRARLNTTRTARLRQLEHMENDEVALQRKVRTEVFQLEDTLAQVKKEYEMLRIEFEQTLAANEQAGPINREMRHLISTLQTHNQQLKGEVVKYKLRLRETQAELHQIRASKGSAVLQSQSSTEMDVKDEMTSPSTPAVSGEPAVKAEPDNGSSTPSSTGISVKTEPGTEPDASIKEEEKEERKEKEEKKEKDFIKKEEKDREREREKEKERERPTRSSSSSSGMKEEREKPGVSSQQEETAGDRLAMLGGSKRKEMEQLKIVRAELKKAQESQREMKLLLDMYRSAPKEQRDKVQLMAAEKKTKSEAEELRQRLRELEERERREGKKMADEEALRKIRSVEEQIDILNKKLSIAKQEEDALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQLLTLKTQVDAQLQVVRKLEEKERLLQGTISTAERELGLRTQALDMNKRKAQESALLSEELRAQLEQVQQKLKLVREEVVENSISREKESFNARRAQEDISKLRRKIEKAKKPAEKISNGDEILNEEINEYKARLTCPCCNSRVKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	XM_012855652.2
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus E3 ubiquitin-protein ligase BRE1A (LOC105920143), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012855652.2

ORF Insert Sequence:

ATGTCGGGGC AGAAGCGTCC AGCTGACCCC AGCGGGCCCT CTTCCTCCGG TGCCCCCGCA 
GAGAAGCGGA GGGAGCGGGA TGGAGAGGAC GGAGGTCCCG GTGTGAGCGC CGGAGCCGGA 
GGGAGCACCG CGGTGGAGAC GGTCATCAAA CTGGGAGGAG TGTCCAACTC GGAGGAACAA 
GACATAAAAG CTCTCCAAGC TAAGAACCGA AAGTTGGGAG AATCTCTCGA TCAAAGACAG 
GTGATCGAGG ACGAGCTGCG AGAGCGAATT GAGAGACTGG AGACCCGACA GGCCACGGAC 
GACGCCAGTT TGCTGATTCT GAACAGATAC TGGAACCAGT TTGATGAGAA CATCCGGCTC 
CTCATCCGGC GCTATGACCA AGCGAGCAGC GAACCGGAGA AATCTCCAAC AGGCGAGGGA 
CGCAGCCTGA AGCCGGAAAC TCCAGAACCC GATGGAGATT CGAACCAGGA GCGGGCCAAA 
GACAGAGGAC ATCAAGGGGA GACGACAAAC TCCTTTTTGG CCACGCTGGC GAGCAGCACC 
AGCGAGGAGA TGGAGGCCGA GCTCCAGGAG AGGGTGGAGT CCAGTCAGAA GCAGGCCAAT 
CGTGTGGTGG AGATCTACAA GAACCTGAAG AGCACCTTGG ACCAGCTGAA AGCAGAGCTT 
GACTCAGGGA CTGAGGGTGG TCTGAAGCAG GTCGCAGCCA AGCTAAACTC CCTCCTGAGC 
CGGGAAAACG AGCTGCTTCA ACAGCTGACC GAGGACCTCA AACAGAAACA CAGCCACATG 
ACCAACGAGT CCCGGTCGTT GGGCCGGGCG GCTAACAAAG CGGACCAGCG CGTCAGCGAG 
CTGCAGGCTC GGATCGAGGA GCTCCAGTGG GACATGGAGA AGATCCGCCG TCGAGAGAAC 
AGGCTGAATG CCCACCTGAG TGAAATACTG GAGAGGGTGA ACAGCAAAGG CTACAAAGTG 
TGTGGCGAGG CCAGCAGTGT GTGTGGAACC ATCACCATTA ACAAGAGAAA GTTTGAGGAG 
ATGAACAGCG AGTTGGAGGA GAACACGGAA CTGGCAGAAA ATCGGCTGGT TGAGCTGCAG 
AAGCTCCAGC AGGACCTGCA AAACGTCCAG CAGGAGAACA ACAGCATGAA GACGGAGCTC 
GTGAGTCGAG CTGAGGGCCT GGTCAAAGAA TCCTCAGAGT ATCGCTGCCT CCAGTCCCAG 
TTTTCCGTGC TCTACAACGA GTCCCTGACC CTCAAGGCCC AGCTAGACGA AACCCGGGCT 
CGCCTCAACA CTACCCGCAC GGCTCGCCTC AGACAGCTGG AGCACATGGA GAACGACGAG 
GTGGCTCTGC AGAGGAAGGT CCGCACCGAA GTGTTTCAGC TGGAGGACAC GTTGGCTCAG 
GTCAAGAAGG AGTACGAGAT GTTGCGCATT GAATTTGAAC AGACTCTTGC CGCCAACGAG 
CAAGCAGGTC CCATCAACAG GGAGATGCGT CACCTTATAA GCACGCTGCA GACACACAAC 
CAGCAGCTGA AAGGAGAGGT GGTGAAGTAC AAGCTGAGAC TGAGAGAGAC CCAAGCTGAG 
CTCCACCAGA TCCGTGCCTC GAAGGGCAGC GCCGTCCTCC AGTCGCAGTC GAGCACGGAG 
ATGGACGTGA AGGACGAGAT GACCTCTCCT TCGACCCCGG CCGTTTCTGG AGAACCCGCG 
GTCAAAGCGG AGCCGGACAA CGGCTCCTCT ACACCCAGCA GCACCGGGAT TTCAGTGAAA 
ACAGAACCTG GAACTGAACC GGACGCAAGC ATAAAAGAGG AGGAAAAAGA GGAGAGAAAA 
GAGAAGGAAG AGAAGAAAGA GAAAGACTTT ATAAAGAAAG AGGAGAAAGA CAGGGAGAGA 
GAACGGGAGA AGGAGAAGGA GAGAGAGAGG CCCACCCGGA GCAGCAGCAG CAGCAGCGGG 
ATGAAGGAGG AGAGGGAGAA GCCGGGCGTC AGCAGCCAGC AGGAGGAGAC GGCCGGGGAT 
CGGCTCGCGA TGCTCGGAGG CTCAAAGAGG AAAGAGATGG AGCAGCTGAA AATCGTCCGA 
GCAGAACTAA AGAAAGCCCA AGAGTCCCAG AGAGAGATGA AGCTTCTGCT GGACATGTAC 
CGATCAGCAC CAAAGGAGCA GAGGGACAAA GTCCAGCTTA TGGCTGCAGA GAAAAAGACC 
AAGTCAGAGG CAGAGGAGCT GCGGCAGAGG CTGCGGGAGC TGGAGGAGAG GGAGAGAAGG 
GAGGGCAAGA AAATGGCGGA CGAAGAAGCT CTGAGGAAGA TCCGCTCAGT CGAAGAACAG 
ATCGACATCC TCAACAAGAA GCTGTCCATC GCCAAACAGG AGGAAGACGC GCTGCTGAGC 
GAGATGGACG TGACGGGCCA GGCCTTCGAG GACATGCAGG AACAGAATAT CCGCCTCATG 
CAGCAGCTCC GAGAAAAGGA CGATGCCAAC TTCAAGCTGA TGAGCGAGCG GATCAAGTCC 
AACCAGATCC ACAAGCTCCT GAAGGAGGAA AAGGAAGAGC TAGCCGACCA ACTGCTAACT 
TTAAAAACAC AGGTAGATGC ACAGCTGCAG GTAGTCAGGA AGCTGGAGGA GAAGGAGCGC 
CTCCTGCAGG GGACTATTAG CACTGCGGAG AGAGAGCTGG GACTTCGAAC ACAAGCTTTG 
GACATGAACA AGCGAAAGGC TCAGGAGTCG GCGCTGCTGT CGGAGGAGCT GCGCGCTCAG 
CTGGAGCAGG TCCAGCAGAA GCTGAAGCTG GTCAGAGAGG AGGTCGTTGA GAACAGCATC 
TCCAGAGAGA AGGAGTCTTT CAATGCCCGG CGGGCGCAGG AGGACATCTC CAAGCTCAGG 
AGAAAGATTG AGAAGGCCAA GAAACCGGCT GAGAAGATAA GCAACGGAGA TGAGATCCTG 
AACGAAGAAA TAAATGAGTA CAAGGCTCGG CTGACGTGCC CGTGCTGCAA CTCCCGGGTG 
AAGGATGCCG TCCTGACAAA ATGCTTCCAC GTCTTCTGCT TCGAGTGCGT CAAGACGCGC 
TACGACACCC GGCAGAGGAA GTGCCCCAAG TGCAATGCCG CCTTCGGGGC GAACGACTTC 
CATCGCATTT ACATCGGCTG A