Bub1 cDNA ORF clone, Fukomys damarensis(Damara mole-rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bub1
Entrez Gene ID	104872008
Full Name	BUB1 mitotic checkpoint serine/threonine kinase
Gene Type	protein-coding
Organism	Fukomys damarensis(Damara mole-rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010638170.1	XP_010636472.1	mitotic checkpoint serine/threonine-protein kinase BUB1

Pathways from BioSystems
Gene Ontology

The following Bub1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bub1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFu72246	XM_010638170.1 Latest version!	Fukomys damarensis BUB1 mitotic checkpoint serine/threonine kinase (Bub1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bub1 ( XM_010638170.1 ) cDNA ORF clone, Fukomys damarensis(Damara mole-rat) -> XP_010636472.1 Fukomys damarensis BUB1 mitotic checkpoint serine/threonine kinase (Bub1), mRNA.

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CloneID	OFu72246
Clone ID Related Accession (Same CDS sequence)	XM_010638170.1
Accession Version	XM_010638170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-23
Organism	Fukomys damarensis(Damara mole-rat)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011045538.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fukomys damarensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAACC CGGAAAATGT CTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
AATGACCCGC TTGGAGAATG GGAAAGTTAC ATGCAATGGG TAGAAGAGAA TTTTCCTAAT 
AATAAAGAAT ACTTGGTAAT GTTATTAGAA CATCTAATGA AGGAATTTTT AGATAAGAAG 
AAATACCACA GTGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT 
GATCTTCATC AGTTTTTTGA GTTTTTGTAC AACCAGGGGA TTGGAACCCA GTCATCTCCT 
CTGTACATAT CCTGGGCTGG GCATCTGGAA GCCCAAGGAC AACTGCAGCA CGCGAGTGCC 
ATTTTTCGGA GAGGAATTCA AAACCAGGCT GAACCTAGAG AGCTATTGCA ACAGCAGTAC 
AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTGAAGCTAG AACCTCAGAA 
CCTCTGCATA ATGCTCAAAT TTTAAACCAA ATGGCATCAT CAAAATTAAG TCCAGTAAAG 
AACTCAGCCT GTGTTTCTAA GAATCAGGGC TCAGAAATTT CTAGAATGAT ACCTTCAGTT 
CCTGATAAAG AATCAAATAT GGAACAAAGG GTGATCATGA TTTCTAAATC AGAATACTCC 
ACACACTCAT CATTGGCAGC AAAAGTTGAT GTTCAGCAAG TTGTTATGTA CTGCAAGGAG 
AAGCTTATTT GTGGAGACTC AGAATTTTCC TTTGAAGAAC TGAGAGCCCA GAAATACAAC 
CAGCGGAGGA AGCATGAACA ATGGGGAAAT GAAGACAGAC ATTATATAAA AAGGAAAGAA 
GCAAATGCGT TTGAAGAACA ACTGTTAAAA CAGAAAATGG ATGCACTCCA TAGGAAATTG 
CATCAAGTGG TGGAGTTATC CCAGGAGGAC CTGCCCATTC CCCAGGAAAG CTCTGAGATT 
AATCCAGTAC TTACAAGACC AAGTGTAAGC TCCCAGCAAG AACAGAGAGC TCCGTGTCTT 
CCAGCCATCA ATCAGCACAC CTCTAAAAAC TTGGGAGAGA AACTCGGAGA GGTGCCTCCT 
ACCATTCCTC TTTTGTCACA TGCTGCTTTG ACGTCCCCAG CTATTGACCA GAGTGTAGCT 
CCTCCTGTTC CTTTGATGGC CCAGCCAGTG ACAAACTCCC GATGTATGAG TCTAAATTCT 
CATGAACTCA AGCTACAGAG TGGATCTGAA ACAAAGGAAG AATGTGAAAC ATATAAGGTC 
ACTAGCACAA GTTCTTTTCA CACTACTCCA AACACATCAC TGGGAATGAT GGTTCAGGCA 
ACACCATCCA AAGTGCAGCC ATCACCCACT GTGCACACGA AGGAAGCATT AGGTTTCATC 
ATGAATGTGT TTCAGGCACC TACGCTTCCT GATATTTCTG ATAACAAAGA TGAATGGCCA 
TCTCTAGATC AAAATGAAGA TGCATTTGAG GCCCAGTTTC AAAAAAATGC AGATTCCACT 
GGGACTTGGG GAGTCAATAA GATTATTTCT TTGCCATCTG GTTTTCCTAT TTTTGAAGAT 
GGAAACAAAG AAAATTATGG ATTACCACAG CCTAAAAATA AGCCGATAGG AGCCAGAACC 
TTTGGAGAAC GCTCTATCAG TGCATTTCCT TCAAAACCAA AAGAAGTGCC TCAGCCTCAC 
TCTGAAGAGT TTTTGGATGA TTCAACCGTC TGGGGTATTC GCTACAACAA ATCCCTGGCA 
CCCAGTCCCA AGAGCATAGG AGACTTCACA TCTGCTGCCC GGCTTGCATC TACACCATTC 
CACAAACTTC CAGCAGATCC AGTGCACATT CTAGAAGATA AAGAAAATGT GGTAGCAACA 
CAGTGCACCC CTATACTGGT GGATTCCTGT AAAGAAAACA TGGTGCAGCC TTCAAAAGGG 
AAGAAGTTCA GCCCAATTCA AGAGGAAAGC CCAGAACAAG CACTCCCAAC AGACATATCT 
ACAGCATCCT TACACCATCC TAGCCAGTCT GCTGCAGGTG GTGTGCTCAC CCAGGAAGCA 
GTGAGGGGCC TTGAGGCTTG TAAACTCACA GACACTAACC TTGTCGTCAT GGAGGCCCCA 
CTGGCAATCA GTGCTGAACT CCACACAGAA TGGATGCAGA CCAATTTGCT TAGGAATGTC 
AAATCTCCAG ACTTCACTAT TGAAAACCCA TGGGATGAAG AATTGATTCT CAAACTTTTA 
TCTGGGCTTT CTAAACCAGT GAGTTCCTAC CCAAATACTT TTGAATGGCA ATGTAAACTT 
CCAGCTATCA AGCCCAAGAC TGAATTTCAG TTAGGTTCTT TGCTAGTCTA CGTCAATCAC 
CTTCTTGGAA CAGGTGCCTT TGCTCAAGTC TACGAAGCTA CCCATGGAGA TGTGAATGAT 
ATCAAAAATA AACAGAAGTG TGTTTTAAAG GTCCAGAAGC CTGCCAGCCC GTGGGAATTC 
TACATTGGGA CTCAGCTGAT GGAGAGATTA AAGCCAACTG CACGACACAT GTTTATCAAA 
TTCTATTCTG CCCATTTATT TCAGAATGGT AGCATTTTAG TTGGCGATCT CTATAGCTAT 
GGAACATTAT TAAATGCCAT TAACCTCTAT AAAAATACCC CTGAAAAAGT GATGCCTCAA 
GCTCTCGTCA TTGCTTTTGC TATCAGAATG CTCTACATGA TTGAACAAGT CCATAACTGT 
GAAATCATTC ATGGAGACAT TAAGCCCGAC AATTTCATAC TGGGAAATAG ATTTTTGGAG 
CAGGACGATG AAGATGAAGA TTTAGCTGCT GGCTTGGCAC TGATTGACTT GGGTCAGAGT 
ATAGATATGA AACTTTTTCC CAAAGGAACT ACATTTACAG CAAGGTGTGA AACATCTGGT 
TTTCAGTGTA TTGAGATGCT CAGTAATAAA CCATGGAACT ACCAGGTTGA TTACTTTGGT 
GTTGCTGCAA CAGTATATTG CATGCTTTTT GGCACTTATA TGAAAGTAAA AAATGAGGGA 
GGAGTCTGGA AGCCTGATGG TATTTTTAGA AGGCTTCCGC ATCTGGATAT GTGGAATGAA 
TTTTTTCACA TCATGCTGAA TATACCAGAT TGTCATCATC TTCCATCTTT GGATTTGTTA 
AGGCAAAAGC TGAAAAAACT GTTTGAGGAA CAGTATGCAA ACAAGATTAA AACCCTGCGT 
AACAGGCTAA TTGTACTTCT CTTGGAATAT AAGCGTTTAA GAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010636472.1
CDS	72..3299
Translation	MDNPENVFQMFEAHMQSYKGNDPLGEWESYMQWVEENFPNNKEYLVMLLEHLMKEFLDKKKYHSDPRFISYCLKFAEYNSDLHQFFEFLYNQGIGTQSSPLYISWAGHLEAQGQLQHASAIFRRGIQNQAEPRELLQQQYRLFQTRLTETHLPAEARTSEPLHNAQILNQMASSKLSPVKNSACVSKNQGSEISRMIPSVPDKESNMEQRVIMISKSEYSTHSSLAAKVDVQQVVMYCKEKLICGDSEFSFEELRAQKYNQRRKHEQWGNEDRHYIKRKEANAFEEQLLKQKMDALHRKLHQVVELSQEDLPIPQESSEINPVLTRPSVSSQQEQRAPCLPAINQHTSKNLGEKLGEVPPTIPLLSHAALTSPAIDQSVAPPVPLMAQPVTNSRCMSLNSHELKLQSGSETKEECETYKVTSTSSFHTTPNTSLGMMVQATPSKVQPSPTVHTKEALGFIMNVFQAPTLPDISDNKDEWPSLDQNEDAFEAQFQKNADSTGTWGVNKIISLPSGFPIFEDGNKENYGLPQPKNKPIGARTFGERSISAFPSKPKEVPQPHSEEFLDDSTVWGIRYNKSLAPSPKSIGDFTSAARLASTPFHKLPADPVHILEDKENVVATQCTPILVDSCKENMVQPSKGKKFSPIQEESPEQALPTDISTASLHHPSQSAAGGVLTQEAVRGLEACKLTDTNLVVMEAPLAISAELHTEWMQTNLLRNVKSPDFTIENPWDEELILKLLSGLSKPVSSYPNTFEWQCKLPAIKPKTEFQLGSLLVYVNHLLGTGAFAQVYEATHGDVNDIKNKQKCVLKVQKPASPWEFYIGTQLMERLKPTARHMFIKFYSAHLFQNGSILVGDLYSYGTLLNAINLYKNTPEKVMPQALVIAFAIRMLYMIEQVHNCEIIHGDIKPDNFILGNRFLEQDDEDEDLAAGLALIDLGQSIDMKLFPKGTTFTARCETSGFQCIEMLSNKPWNYQVDYFGVAATVYCMLFGTYMKVKNEGGVWKPDGIFRRLPHLDMWNEFFHIMLNIPDCHHLPSLDLLRQKLKKLFEEQYANKIKTLRNRLIVLLLEYKRLRK

Target ORF information:

RefSeq Version	XM_010638170.1
Organism	Fukomys damarensis(Damara mole-rat)
Definition	Fukomys damarensis BUB1 mitotic checkpoint serine/threonine kinase (Bub1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010638170.1

ORF Insert Sequence:

ATGGATAACC CGGAAAATGT CTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
AATGACCCGC TTGGAGAATG GGAAAGTTAC ATGCAATGGG TAGAAGAGAA TTTTCCTAAT 
AATAAAGAAT ACTTGGTAAT GTTATTAGAA CATCTAATGA AGGAATTTTT AGATAAGAAG 
AAATACCACA GTGACCCAAG ATTCATCAGT TATTGTTTAA AATTTGCTGA GTACAACAGT 
GATCTTCATC AGTTTTTTGA GTTTTTGTAC AACCAGGGGA TTGGAACCCA GTCATCTCCT 
CTGTACATAT CCTGGGCTGG GCATCTGGAA GCCCAAGGAC AACTGCAGCA CGCGAGTGCC 
ATTTTTCGGA GAGGAATTCA AAACCAGGCT GAACCTAGAG AGCTATTGCA ACAGCAGTAC 
AGGTTATTTC AGACACGCCT CACTGAAACC CATTTGCCAG CTGAAGCTAG AACCTCAGAA 
CCTCTGCATA ATGCTCAAAT TTTAAACCAA ATGGCATCAT CAAAATTAAG TCCAGTAAAG 
AACTCAGCCT GTGTTTCTAA GAATCAGGGC TCAGAAATTT CTAGAATGAT ACCTTCAGTT 
CCTGATAAAG AATCAAATAT GGAACAAAGG GTGATCATGA TTTCTAAATC AGAATACTCC 
ACACACTCAT CATTGGCAGC AAAAGTTGAT GTTCAGCAAG TTGTTATGTA CTGCAAGGAG 
AAGCTTATTT GTGGAGACTC AGAATTTTCC TTTGAAGAAC TGAGAGCCCA GAAATACAAC 
CAGCGGAGGA AGCATGAACA ATGGGGAAAT GAAGACAGAC ATTATATAAA AAGGAAAGAA 
GCAAATGCGT TTGAAGAACA ACTGTTAAAA CAGAAAATGG ATGCACTCCA TAGGAAATTG 
CATCAAGTGG TGGAGTTATC CCAGGAGGAC CTGCCCATTC CCCAGGAAAG CTCTGAGATT 
AATCCAGTAC TTACAAGACC AAGTGTAAGC TCCCAGCAAG AACAGAGAGC TCCGTGTCTT 
CCAGCCATCA ATCAGCACAC CTCTAAAAAC TTGGGAGAGA AACTCGGAGA GGTGCCTCCT 
ACCATTCCTC TTTTGTCACA TGCTGCTTTG ACGTCCCCAG CTATTGACCA GAGTGTAGCT 
CCTCCTGTTC CTTTGATGGC CCAGCCAGTG ACAAACTCCC GATGTATGAG TCTAAATTCT 
CATGAACTCA AGCTACAGAG TGGATCTGAA ACAAAGGAAG AATGTGAAAC ATATAAGGTC 
ACTAGCACAA GTTCTTTTCA CACTACTCCA AACACATCAC TGGGAATGAT GGTTCAGGCA 
ACACCATCCA AAGTGCAGCC ATCACCCACT GTGCACACGA AGGAAGCATT AGGTTTCATC 
ATGAATGTGT TTCAGGCACC TACGCTTCCT GATATTTCTG ATAACAAAGA TGAATGGCCA 
TCTCTAGATC AAAATGAAGA TGCATTTGAG GCCCAGTTTC AAAAAAATGC AGATTCCACT 
GGGACTTGGG GAGTCAATAA GATTATTTCT TTGCCATCTG GTTTTCCTAT TTTTGAAGAT 
GGAAACAAAG AAAATTATGG ATTACCACAG CCTAAAAATA AGCCGATAGG AGCCAGAACC 
TTTGGAGAAC GCTCTATCAG TGCATTTCCT TCAAAACCAA AAGAAGTGCC TCAGCCTCAC 
TCTGAAGAGT TTTTGGATGA TTCAACCGTC TGGGGTATTC GCTACAACAA ATCCCTGGCA 
CCCAGTCCCA AGAGCATAGG AGACTTCACA TCTGCTGCCC GGCTTGCATC TACACCATTC 
CACAAACTTC CAGCAGATCC AGTGCACATT CTAGAAGATA AAGAAAATGT GGTAGCAACA 
CAGTGCACCC CTATACTGGT GGATTCCTGT AAAGAAAACA TGGTGCAGCC TTCAAAAGGG 
AAGAAGTTCA GCCCAATTCA AGAGGAAAGC CCAGAACAAG CACTCCCAAC AGACATATCT 
ACAGCATCCT TACACCATCC TAGCCAGTCT GCTGCAGGTG GTGTGCTCAC CCAGGAAGCA 
GTGAGGGGCC TTGAGGCTTG TAAACTCACA GACACTAACC TTGTCGTCAT GGAGGCCCCA 
CTGGCAATCA GTGCTGAACT CCACACAGAA TGGATGCAGA CCAATTTGCT TAGGAATGTC 
AAATCTCCAG ACTTCACTAT TGAAAACCCA TGGGATGAAG AATTGATTCT CAAACTTTTA 
TCTGGGCTTT CTAAACCAGT GAGTTCCTAC CCAAATACTT TTGAATGGCA ATGTAAACTT 
CCAGCTATCA AGCCCAAGAC TGAATTTCAG TTAGGTTCTT TGCTAGTCTA CGTCAATCAC 
CTTCTTGGAA CAGGTGCCTT TGCTCAAGTC TACGAAGCTA CCCATGGAGA TGTGAATGAT 
ATCAAAAATA AACAGAAGTG TGTTTTAAAG GTCCAGAAGC CTGCCAGCCC GTGGGAATTC 
TACATTGGGA CTCAGCTGAT GGAGAGATTA AAGCCAACTG CACGACACAT GTTTATCAAA 
TTCTATTCTG CCCATTTATT TCAGAATGGT AGCATTTTAG TTGGCGATCT CTATAGCTAT 
GGAACATTAT TAAATGCCAT TAACCTCTAT AAAAATACCC CTGAAAAAGT GATGCCTCAA 
GCTCTCGTCA TTGCTTTTGC TATCAGAATG CTCTACATGA TTGAACAAGT CCATAACTGT 
GAAATCATTC ATGGAGACAT TAAGCCCGAC AATTTCATAC TGGGAAATAG ATTTTTGGAG 
CAGGACGATG AAGATGAAGA TTTAGCTGCT GGCTTGGCAC TGATTGACTT GGGTCAGAGT 
ATAGATATGA AACTTTTTCC CAAAGGAACT ACATTTACAG CAAGGTGTGA AACATCTGGT 
TTTCAGTGTA TTGAGATGCT CAGTAATAAA CCATGGAACT ACCAGGTTGA TTACTTTGGT 
GTTGCTGCAA CAGTATATTG CATGCTTTTT GGCACTTATA TGAAAGTAAA AAATGAGGGA 
GGAGTCTGGA AGCCTGATGG TATTTTTAGA AGGCTTCCGC ATCTGGATAT GTGGAATGAA 
TTTTTTCACA TCATGCTGAA TATACCAGAT TGTCATCATC TTCCATCTTT GGATTTGTTA 
AGGCAAAAGC TGAAAAAACT GTTTGAGGAA CAGTATGCAA ACAAGATTAA AACCCTGCGT 
AACAGGCTAA TTGTACTTCT CTTGGAATAT AAGCGTTTAA GAAAATAA