AYL99_04901 cDNA ORF clone, Fonsecaea erecta

Gene
Clones
gRNAs

Summary

Gene Symbol	AYL99_04901
Entrez Gene ID	30009069
Full Name	Amidase
Gene Type	protein-coding
Organism	Fonsecaea erecta

mRNA and Protein(s)

mRNA	Protein	Name
XM_018836413.1	XP_018693266.1	Amidase

Pathways from BioSystems
Gene Ontology

The following AYL99_04901 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AYL99_04901 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFo17241	XM_018836413.1 Latest version!	Fonsecaea erecta Amidase (AYL99_04901), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AYL99_04901 ( XM_018836413.1 ) cDNA ORF clone, Fonsecaea erecta -> XP_018693266.1 Fonsecaea erecta Amidase (AYL99_04901), partial mRNA.

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CloneID	OFo17241
Clone ID Related Accession (Same CDS sequence)	XM_018836413.1
Accession Version	XM_018836413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-27
Organism	Fonsecaea erecta
Product	Amidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_017387247). COMPLETENESS: incomplete on both ends.

ATGTCTGTTT TTTCACTCGA TGTGTCCAAG GATAATCCAG TGACCACGGA CGACCTTGAT 
CGGGCATGCG AAATACTTGG AGTCTCCATC AAAAATGAGG AAAAGGCAGA GTATGTCAAT 
CTCTTGGCCG TATACCACGA TTCTATGGCC AAGCTGATGG CACTCGAAGA CTATATCCCA 
CAAGTTGACC ATGAGAGGTT CGGAAGGAGG AATGTCCATT TTCCTCAGCC TGCTGACAAC 
CCCTACGGAG CGTGGGCTTG GAGGTGTGAG ATCTCCGACA GGACTCCCAA TCCAAATGCA 
CTCCTTCATG GCAAGACGGT AGCACTGAAG GACTGTATTG CGGTCGAGGG CGTGCCAATG 
CTGCTTGGCA CAAACTTCTT TTCAGACTAT ACTCCGAGCA CTGATGCGGC TATTGTCACT 
CGTATGTTGA AGCATGGCGC GACTATCTTG GGTAAGTCAG TCTGTGAAAA CCTGTGTCAT 
TCGGCGACGT CACATACATC ATCGACGGGA CCCGTGCATA ATCCATACGC AAAGGGGTAT 
GCTGCAGGAG GTTCTTCGTC AGGTTCCGCA GTCTTGGTCT CTCTTGGCCC TGAGAATGGA 
GGCGTGGATT ACGCTATAGG GGCAGATCAA GGCGGGAGCA TCAGGGTCCC TGCGGCGTGG 
TGCGGTATCG TCGGATTCAA ACCAACCTTT GGTCTCATTC CCTGGACGGG CGCGGGCAGC 
AATGAGCCTA CAAATGATCA CATGGGACCG ATGACACGCG ACGTCCTGAG CAATGCCCGC 
CTGCTGAAGG CTATAGCAGG GACAGACTTT GTGGATGATC GGTCATACGG TGCTCCGCCG 
CCAGAGAAGA TTCCAGCCTA TCACGAAATT CTCATCGGGC AGCAAAAGCC TCAAGACCTT 
ACAGGTATGA AGGTCGGTAT TCTGGTCGAG GGTCTCCGCA CCCCGGCGCT CGATCCGAGA 
GTAAAGGAGT TGTTTCTCGC TGCTGCGGAC AAGTTTCGAA GCCTAGGTGC AGAAGTGGAA 
GAAGTATCTG TCCCGACACA TGCCACGGCA AGTGCAGTTT GGACTGGCAT CTCGAAGTTG 
GGAGGCTATG TGAATAAGAC ACTGGCGCTG GGAGGCCGCA GAGGCTACCA ACTCAACGAT 
CTCAATCAGA AGCTATGGCC CATGACTCAG GACAAATGGG ACAATGCCAT CCCATCGACC 
AAAAACATCT ACCTGAACGG CGTCTATGCT CAGGACATGC AGAAATTCCC AGGCTTGTTT 
GGTAAAGCAA CAAATCTCTC ACTCAAGGCG CGTCATGGCT ACGATGCAGC GCTCGGCGAC 
TGGTCGTCAA TATCAACCTT TGTCACCGAC TCCAAACTCG AAGAAATGAA GCAGCAGAAG 
ACATTCGACG TCTTGATCTT ACCAACATTG CCATTCGTGG CCAAATCACA CCCTGAGCTC 
GGCGAATCAG GGGCAATAGG GAATCCCATA TTGCAGCTGC AGAAGCAAGT CGGTTTGACC 
GGAAATACAG CTCCTTTCAA TCAGACCGGC CACCCTGCTC TGGCCATGCC TGTGGGGATG 
CTTGAGCCTA CCGAGGGACC ACTGGCGGGA ACTCCAACCA AACTGCCCGT CAGCATGCAA 
ATCGTAGGCA AATGGTGGGA CGAGTTGACG GTGCTCCGAG TAGCGTACGC CTGGGAGACT 
GCTGCTGGAG GTCCAAACGC CTGGAAGGAG TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018693266.1
CDS	1..1716
Translation	MSVFSLDVSKDNPVTTDDLDRACEILGVSIKNEEKAEYVNLLAVYHDSMAKLMALEDYIPQVDHERFGRRNVHFPQPADNPYGAWAWRCEISDRTPNPNALLHGKTVALKDCIAVEGVPMLLGTNFFSDYTPSTDAAIVTRMLKHGATILGKSVCENLCHSATSHTSSTGPVHNPYAKGYAAGGSSSGSAVLVSLGPENGGVDYAIGADQGGSIRVPAAWCGIVGFKPTFGLIPWTGAGSNEPTNDHMGPMTRDVLSNARLLKAIAGTDFVDDRSYGAPPPEKIPAYHEILIGQQKPQDLTGMKVGILVEGLRTPALDPRVKELFLAAADKFRSLGAEVEEVSVPTHATASAVWTGISKLGGYVNKTLALGGRRGYQLNDLNQKLWPMTQDKWDNAIPSTKNIYLNGVYAQDMQKFPGLFGKATNLSLKARHGYDAALGDWSSISTFVTDSKLEEMKQQKTFDVLILPTLPFVAKSHPELGESGAIGNPILQLQKQVGLTGNTAPFNQTGHPALAMPVGMLEPTEGPLAGTPTKLPVSMQIVGKWWDELTVLRVAYAWETAAGGPNAWKEF

Target ORF information:

RefSeq Version	XM_018836413.1
Organism	Fonsecaea erecta
Definition	Fonsecaea erecta Amidase (AYL99_04901), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018836413.1

ORF Insert Sequence:

ATGTCTGTTT TTTCACTCGA TGTGTCCAAG GATAATCCAG TGACCACGGA CGACCTTGAT 
CGGGCATGCG AAATACTTGG AGTCTCCATC AAAAATGAGG AAAAGGCAGA GTATGTCAAT 
CTCTTGGCCG TATACCACGA TTCTATGGCC AAGCTGATGG CACTCGAAGA CTATATCCCA 
CAAGTTGACC ATGAGAGGTT CGGAAGGAGG AATGTCCATT TTCCTCAGCC TGCTGACAAC 
CCCTACGGAG CGTGGGCTTG GAGGTGTGAG ATCTCCGACA GGACTCCCAA TCCAAATGCA 
CTCCTTCATG GCAAGACGGT AGCACTGAAG GACTGTATTG CGGTCGAGGG CGTGCCAATG 
CTGCTTGGCA CAAACTTCTT TTCAGACTAT ACTCCGAGCA CTGATGCGGC TATTGTCACT 
CGTATGTTGA AGCATGGCGC GACTATCTTG GGTAAGTCAG TCTGTGAAAA CCTGTGTCAT 
TCGGCGACGT CACATACATC ATCGACGGGA CCCGTGCATA ATCCATACGC AAAGGGGTAT 
GCTGCAGGAG GTTCTTCGTC AGGTTCCGCA GTCTTGGTCT CTCTTGGCCC TGAGAATGGA 
GGCGTGGATT ACGCTATAGG GGCAGATCAA GGCGGGAGCA TCAGGGTCCC TGCGGCGTGG 
TGCGGTATCG TCGGATTCAA ACCAACCTTT GGTCTCATTC CCTGGACGGG CGCGGGCAGC 
AATGAGCCTA CAAATGATCA CATGGGACCG ATGACACGCG ACGTCCTGAG CAATGCCCGC 
CTGCTGAAGG CTATAGCAGG GACAGACTTT GTGGATGATC GGTCATACGG TGCTCCGCCG 
CCAGAGAAGA TTCCAGCCTA TCACGAAATT CTCATCGGGC AGCAAAAGCC TCAAGACCTT 
ACAGGTATGA AGGTCGGTAT TCTGGTCGAG GGTCTCCGCA CCCCGGCGCT CGATCCGAGA 
GTAAAGGAGT TGTTTCTCGC TGCTGCGGAC AAGTTTCGAA GCCTAGGTGC AGAAGTGGAA 
GAAGTATCTG TCCCGACACA TGCCACGGCA AGTGCAGTTT GGACTGGCAT CTCGAAGTTG 
GGAGGCTATG TGAATAAGAC ACTGGCGCTG GGAGGCCGCA GAGGCTACCA ACTCAACGAT 
CTCAATCAGA AGCTATGGCC CATGACTCAG GACAAATGGG ACAATGCCAT CCCATCGACC 
AAAAACATCT ACCTGAACGG CGTCTATGCT CAGGACATGC AGAAATTCCC AGGCTTGTTT 
GGTAAAGCAA CAAATCTCTC ACTCAAGGCG CGTCATGGCT ACGATGCAGC GCTCGGCGAC 
TGGTCGTCAA TATCAACCTT TGTCACCGAC TCCAAACTCG AAGAAATGAA GCAGCAGAAG 
ACATTCGACG TCTTGATCTT ACCAACATTG CCATTCGTGG CCAAATCACA CCCTGAGCTC 
GGCGAATCAG GGGCAATAGG GAATCCCATA TTGCAGCTGC AGAAGCAAGT CGGTTTGACC 
GGAAATACAG CTCCTTTCAA TCAGACCGGC CACCCTGCTC TGGCCATGCC TGTGGGGATG 
CTTGAGCCTA CCGAGGGACC ACTGGCGGGA ACTCCAACCA AACTGCCCGT CAGCATGCAA 
ATCGTAGGCA AATGGTGGGA CGAGTTGACG GTGCTCCGAG TAGCGTACGC CTGGGAGACT 
GCTGCTGGAG GTCCAAACGC CTGGAAGGAG TTCTAG