AYL99_04856 cDNA ORF clone, Fonsecaea erecta

Gene
Clones
gRNAs

Summary

Gene Symbol	AYL99_04856
Entrez Gene ID	30009024
Full Name	methylenetetrahydrofolate reductase
Gene Type	protein-coding
Organism	Fonsecaea erecta

mRNA and Protein(s)

mRNA	Protein	Name
XM_018836368.1	XP_018693221.1	methylenetetrahydrofolate reductase

Pathways from BioSystems
Gene Ontology

The following AYL99_04856 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AYL99_04856 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFo17196	XM_018836368.1 Latest version!	Fonsecaea erecta methylenetetrahydrofolate reductase (AYL99_04856), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AYL99_04856 ( XM_018836368.1 ) cDNA ORF clone, Fonsecaea erecta -> XP_018693221.1 Fonsecaea erecta methylenetetrahydrofolate reductase (AYL99_04856), partial mRNA.

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CloneID	OFo17196
Clone ID Related Accession (Same CDS sequence)	XM_018836368.1
Accession Version	XM_018836368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-27
Organism	Fonsecaea erecta
Product	methylenetetrahydrofolate reductase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_017387247). COMPLETENESS: incomplete on both ends.

ATGCACGTCA CCAAGAAACT CGAGCTGGCT CACCAGCAGA ACAAGCCCAC CTTCTCTTTC 
GAGTTCTTCC CTCCCAAGAC GGCGCAGGGC GTGCAGAATC TGTACGACCG CATGGACCGC 
ATGCACAATT TGGGCCCGGC ATTCGTCGAC ATCACCTGGG GCGCAGGCGG GAGGCATTCC 
CAGTTGACCT CGGAGATGGT CAACGCCAGT CAGAATTTCT ATGGGTTAGA AACGTGCATG 
CATTTGACCT GCACGGACAT GGAACGACAC CAAGTCGACG AAGCACTTCG TCGTGCATAC 
AAGGCAGGCT GTACAAATAT TCTCGCGCTG CGTGGAGATC CGCCCCGCGA CCAAGAAAAG 
TGGGAGGCAA AGGAAGGTGG TTTCCGGTAT GCAAAAGATC TGGTCAAGTA CATCAGAGCA 
AGATACAAGA ACCATTTCGA CATCGGAGTT GCCGGCTACC CCGAGGGCTG CGACGACCAA 
AGGGATGTGG ACGTGCTGAT CGACCATCTC AAGGAAAAGG TCGATGCTGG AGCCAGTTTC 
ATTATCACAC AGATGTTCTA CGATGTCGAT ATCTTTCTTG GCTGGGTGAA GAAATGTCGT 
GATCGGGGAA TCAGTGTTCC GATACTTCCT GGGATCATGC CTATTCAAAC GTACGCTGCG 
TTCGTGAGAC GAGCTAACTG GACCAAATGT CACGTCCCGC CCGGATGGTT CGAGGCCTTG 
GAGCCGGTCA AGAACGACGA CGCTGCCGTC AGGGCCATTG GAAAAGACCT GGTGGCTGAC 
ATGTGCCGCA AGATTCTCAA TTCCGGAATT CAACATCTTC ACTTCTACAC CATGAATCTC 
GCGCAAGCTA CTCGCATGGT CCTCGAGGAT TTGTCGCTGA CACCTGAAAC CTCCGGAATG 
CCACTCGAGA AACCGCTACC CTGGCGCCAG TCTTTGGGAT TCAACCGCCG GGACGAGACG 
GTCAGGCCAA TCTTCTGGAG AAATCGGAAT GCATCGTACA TCAGCAGGAC AGCCGACTGG 
GACGAGTTTC CCAACGGCCG TTGGGGCGAC AGCAGGTCTC CCGCCTTTGG CGAACTGGAT 
GCTTATAGCA TCGGGCTGAA GGGCACCAAC GAAGCCAACA TCAAGCTATG GGGAAAACCA 
AAGTCCCTTC GCGATGTGAC GGGGCTATTC GTACGCTTTC TGCAAGGTGA CTTGGATCGA 
CTCCCGTGGA GCGAAGGTGC CATCTCTGCC GAAGCCGACG TCATCAAGGA TGATTTGCTA 
CAGTTGAACA AGCACGGATT CTTGACCATC AATTCTCAGC CCGCCGTGGA TGGTGCGTCT 
TCCACCCATC CAGTCTATGG ATGGGGTCCT GCAGGCGGCT GGGTCTACCA GAAAGCATAC 
CTGGAACTGC TTGTCTTCCC CTCCATGTTC AAGGCCGTGA TGGACCGACT CAACGCCGAC 
AAAAACCTCA CCTATTACGC GGTAAATCGG AAGGGCGAAC TGTACACAAA CACCAAGGAC 
GAAGGTCCGA ACGCCGTCAC TTGGGGCATC TTTCCGAACC GCGAGATCGT GCAGCCAACC 
ATCGTGGAAA CAGTTTCCTT CCTGGCCTGG AAAGACGAAG CGTTCCGTCT CGGGCAGGAT 
TGGGCGAACT GCTACCCTGC GGGTTCCGGA AGCAGAAAGC TGATCGAGAA AATCATGGAC 
GAGTGCTACC TGGTCAACAT TGTCAACAAC GATTTCCACC AAAACAAGGA GATCTTCAAG 
GTGTTTGAGG GTCTAGAGAC CAAGGATCTC GATTTAGTCA TCGACGACAA TGCTTCCGGT 
CACATAGAAA ACGGGACCAA AGACGTCCCA AACGGTGACG CTGAGAAAGG CCTTACGAAC 
GGGGCACAAC GCAAAGCCCC TGTCAAGGCC GATGGAGAGG TTCTCTCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018693221.1
CDS	1..1914
Translation	MHVTKKLELAHQQNKPTFSFEFFPPKTAQGVQNLYDRMDRMHNLGPAFVDITWGAGGRHSQLTSEMVNASQNFYGLETCMHLTCTDMERHQVDEALRRAYKAGCTNILALRGDPPRDQEKWEAKEGGFRYAKDLVKYIRARYKNHFDIGVAGYPEGCDDQRDVDVLIDHLKEKVDAGASFIITQMFYDVDIFLGWVKKCRDRGISVPILPGIMPIQTYAAFVRRANWTKCHVPPGWFEALEPVKNDDAAVRAIGKDLVADMCRKILNSGIQHLHFYTMNLAQATRMVLEDLSLTPETSGMPLEKPLPWRQSLGFNRRDETVRPIFWRNRNASYISRTADWDEFPNGRWGDSRSPAFGELDAYSIGLKGTNEANIKLWGKPKSLRDVTGLFVRFLQGDLDRLPWSEGAISAEADVIKDDLLQLNKHGFLTINSQPAVDGASSTHPVYGWGPAGGWVYQKAYLELLVFPSMFKAVMDRLNADKNLTYYAVNRKGELYTNTKDEGPNAVTWGIFPNREIVQPTIVETVSFLAWKDEAFRLGQDWANCYPAGSGSRKLIEKIMDECYLVNIVNNDFHQNKEIFKVFEGLETKDLDLVIDDNASGHIENGTKDVPNGDAEKGLTNGAQRKAPVKADGEVLSN

Target ORF information:

RefSeq Version	XM_018836368.1
Organism	Fonsecaea erecta
Definition	Fonsecaea erecta methylenetetrahydrofolate reductase (AYL99_04856), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018836368.1

ORF Insert Sequence:

ATGCACGTCA CCAAGAAACT CGAGCTGGCT CACCAGCAGA ACAAGCCCAC CTTCTCTTTC 
GAGTTCTTCC CTCCCAAGAC GGCGCAGGGC GTGCAGAATC TGTACGACCG CATGGACCGC 
ATGCACAATT TGGGCCCGGC ATTCGTCGAC ATCACCTGGG GCGCAGGCGG GAGGCATTCC 
CAGTTGACCT CGGAGATGGT CAACGCCAGT CAGAATTTCT ATGGGTTAGA AACGTGCATG 
CATTTGACCT GCACGGACAT GGAACGACAC CAAGTCGACG AAGCACTTCG TCGTGCATAC 
AAGGCAGGCT GTACAAATAT TCTCGCGCTG CGTGGAGATC CGCCCCGCGA CCAAGAAAAG 
TGGGAGGCAA AGGAAGGTGG TTTCCGGTAT GCAAAAGATC TGGTCAAGTA CATCAGAGCA 
AGATACAAGA ACCATTTCGA CATCGGAGTT GCCGGCTACC CCGAGGGCTG CGACGACCAA 
AGGGATGTGG ACGTGCTGAT CGACCATCTC AAGGAAAAGG TCGATGCTGG AGCCAGTTTC 
ATTATCACAC AGATGTTCTA CGATGTCGAT ATCTTTCTTG GCTGGGTGAA GAAATGTCGT 
GATCGGGGAA TCAGTGTTCC GATACTTCCT GGGATCATGC CTATTCAAAC GTACGCTGCG 
TTCGTGAGAC GAGCTAACTG GACCAAATGT CACGTCCCGC CCGGATGGTT CGAGGCCTTG 
GAGCCGGTCA AGAACGACGA CGCTGCCGTC AGGGCCATTG GAAAAGACCT GGTGGCTGAC 
ATGTGCCGCA AGATTCTCAA TTCCGGAATT CAACATCTTC ACTTCTACAC CATGAATCTC 
GCGCAAGCTA CTCGCATGGT CCTCGAGGAT TTGTCGCTGA CACCTGAAAC CTCCGGAATG 
CCACTCGAGA AACCGCTACC CTGGCGCCAG TCTTTGGGAT TCAACCGCCG GGACGAGACG 
GTCAGGCCAA TCTTCTGGAG AAATCGGAAT GCATCGTACA TCAGCAGGAC AGCCGACTGG 
GACGAGTTTC CCAACGGCCG TTGGGGCGAC AGCAGGTCTC CCGCCTTTGG CGAACTGGAT 
GCTTATAGCA TCGGGCTGAA GGGCACCAAC GAAGCCAACA TCAAGCTATG GGGAAAACCA 
AAGTCCCTTC GCGATGTGAC GGGGCTATTC GTACGCTTTC TGCAAGGTGA CTTGGATCGA 
CTCCCGTGGA GCGAAGGTGC CATCTCTGCC GAAGCCGACG TCATCAAGGA TGATTTGCTA 
CAGTTGAACA AGCACGGATT CTTGACCATC AATTCTCAGC CCGCCGTGGA TGGTGCGTCT 
TCCACCCATC CAGTCTATGG ATGGGGTCCT GCAGGCGGCT GGGTCTACCA GAAAGCATAC 
CTGGAACTGC TTGTCTTCCC CTCCATGTTC AAGGCCGTGA TGGACCGACT CAACGCCGAC 
AAAAACCTCA CCTATTACGC GGTAAATCGG AAGGGCGAAC TGTACACAAA CACCAAGGAC 
GAAGGTCCGA ACGCCGTCAC TTGGGGCATC TTTCCGAACC GCGAGATCGT GCAGCCAACC 
ATCGTGGAAA CAGTTTCCTT CCTGGCCTGG AAAGACGAAG CGTTCCGTCT CGGGCAGGAT 
TGGGCGAACT GCTACCCTGC GGGTTCCGGA AGCAGAAAGC TGATCGAGAA AATCATGGAC 
GAGTGCTACC TGGTCAACAT TGTCAACAAC GATTTCCACC AAAACAAGGA GATCTTCAAG 
GTGTTTGAGG GTCTAGAGAC CAAGGATCTC GATTTAGTCA TCGACGACAA TGCTTCCGGT 
CACATAGAAA ACGGGACCAA AGACGTCCCA AACGGTGACG CTGAGAAAGG CCTTACGAAC 
GGGGCACAAC GCAAAGCCCC TGTCAAGGCC GATGGAGAGG TTCTCTCCAA CTGA